Rroxscaffold_6G00408840

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002694
Physical Location & Seq
Reverse (-)
31340104 .. 31342099
1996 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00408840.1

Sequence Viewer

Length: 1551 bp
ATGCGATTATGTCTACCGGAAACCACAATCGCCTTAATGAACACCACAATCGTAGCGGAAGATGCATTCTACCCTGGCTCAAGAAGCAGAGTTCCTCAAAGCCTCATCAATATCGTCCCAACAGCGATCGCTCCTCAAATGGTCTCCATCGGACCTTTTTACCACGGAAAGGAGTTACAGCTGGAATTTGAAAGCTCCAAATGGTGGTTTCTTAATCGACTCCTTTCTCGAACAAAGAGACATGGCGGACCTGATCGTCTAAGAGATTTGCTTGAAGCCGTACAAAAATTCGAAAAAAGAGCGAGAGCATTCTATGATGAGGACGTCCACATGTCAAGCCCAAAGTTTATCGAAATGATGGTGCTTGATGGATGCTTTATCATTGAGCTGTTTCGACATCTTGGTAGAAATGAAGATGTCTTGGGTTTCGATAATCCCATCTCTACCAAGCCATGGTTGATTCCAATTATCACTAGTGACCTCCTCAAGCTTGAAAACCAACTCCCTTTCTTCGTCCTTGAAGAATTGTTCGAAGTTACTGCCACTGCTGAGGTTGGGGATCCTCTAGCTTTACTTGCCTTGAAGTTCTTCAATCATTCCTTGCCTAGGCATGATGAAGTCCTTAAAAGTCTAGTATTTGATGACTACAGACACTTGCTTGGCTTGTTTCACTACAGCTTCTGTCCCACACTCCCTCCCACTCGATATATAGATAACAAACATCAGCTGTCACATTTCCGGCTGATCCCAGCAGAGCTTCCACCGTTCCTGGCCTTCCCACAGCTCCCTCCGCTCCCAGAACAAAGCGAAGATCACCGAGTCAGTCTACTTAGGTACTGGAGCGTTTCAATCCCCATCTTTTGTTTAAGGTGGAGCTTTTCTACCATTTTTTATTGTTTAGGGTGGAGCATTGCAATTTTAAGACTCCATTATAGTATCCTTTTCTATTGTTTGCGGTGGAGCATTTCAATCCTTGCAAGATTTCTTTCCCTAACTCTACCCAACTCTGAAGAGTATCGCCATCCGGTCCTGTCAGTCCAGTCTACCACACAACTGAGACCCTCTGGGGTCAAGTTTAAGCCACACAAAGCAGACAGCTTCTTAGAGATCAATTTCAGAAATAGGGTTCTACACATCCCACCTATTACCATCAATGACCTCACCATTACCGTTTTTATCAACTGCCTAGCCTATGAACACTTGTACCACTTCCACCGTAACTGCTTCACCCATTATATTGCCTTCTTGAGCTGTCTGATCAATTCACCTAGAGACGTCGCATTCCTTTGTGCTGAAGGGGTTATTGCTAGTTACTCTCACAACGATTATTACATAGCAGGGCTGTTCAACAAATTGGGGGAAAAGTTTATTTTCACAGAAATACTTGTGGCTACCTTGACGGGGAATTCAGAGCCCTGGAGGCCTATTACAACAGCTATTGGGGTACCTTTATGCGAACTTATTTCAGCACTCCATGGTCCTTCATTTCAGTTGTTTCAGCCGTCATCCTTCTTGTCCTCACCGGAGGCCAAACTGTTCTGGCTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

516

Amino Acids

59.57

Weight (kDa)

6.72

Isoelectric Point (pI)

58.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 30 - 458 4.2e-84 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 255
AatII GACGTC 2 cut(s) 327, 1278
Acc65I GGTACC 1 cut(s) 1444
AccB1I GGYRCC 1 cut(s) 1444
AccB7I CCANNNNNTGG 2 cut(s) 204, 453
AccBSI CCGCTC 1 cut(s) 793
AccI GTMKAC 3 cut(s) 13, 826, 1043
AciI CCGC 4 cut(s) 56, 246, 791, 955
AclWI GGATC 3 cut(s) 554, 567, 739
AcsI RAATTY 3 cut(s) 185, 287, 1405
AcuI CTGAAG 2 cut(s) 1029, 1314
AcyI GRCGYC 2 cut(s) 324, 1275
AfaI GTAC 4 cut(s) 282, 836, 1205, 1446
AfiI CCNNNNNNNGG 5 cut(s) 169, 204, 453, 606, 1401
AflIII ACRYGT 1 cut(s) 330
AgsI TTSAA 9 cut(s) 191, 275, 494, 521, 583, 592, 849, 969, 1348
AhlI ACTAGT 1 cut(s) 473
AjnI CCWGG 3 cut(s) 73, 768, 1415
Alw26I GTCTC 4 cut(s) 148, 232, 1051, 1266
AlwI GGATC 3 cut(s) 554, 567, 739
AlwNI CAGNNNCTG 1 cut(s) 681
AoxI GGCC 3 cut(s) 771, 1421, 1527
ApoI RAATTY 3 cut(s) 185, 287, 1405
AsiSI GCGATCGC 1 cut(s) 129
Asp700I GAANNNNTTC 1 cut(s) 587
Asp718I GGTACC 1 cut(s) 1444
AspA2I CCTAGG 1 cut(s) 605
AspS9I GGNCC 4 cut(s) 152, 248, 1027, 1478
AsuHPI GGTGA 5 cut(s) 806, 1153, 1219, 1257, 1512
AsuII TTCGAA 2 cut(s) 291, 531
AvaII GGWCC 4 cut(s) 152, 248, 1027, 1478
AvrII CCTAGG 1 cut(s) 605
BaeI ACNNNNGTAYC 2 cut(s) 1187, 1220
BamHI GGATCC 1 cut(s) 559
BanI GGYRCC 1 cut(s) 1444
BanII GRGCYC 1 cut(s) 1416
BbvCI CCTCAGC 1 cut(s) 549
BccI CCATC 7 cut(s) 155, 352, 362, 446, 863, 1029, 1157
BceAI ACGGC 2 cut(s) 263, 1486
BcgI CGANNNNNNTGC 2 cut(s) 521, 555
BciT130I CCWGG 3 cut(s) 75, 770, 1417
BciVI GTATCC 1 cut(s) 947
BclI TGATCA 1 cut(s) 1257
BcoDI GTCTC 4 cut(s) 148, 232, 1051, 1266
BcuI ACTAGT 1 cut(s) 473
BfaI CTAG 7 cut(s) 474, 566, 606, 632, 1187, 1269, 1308
BfmI CTRYAG 2 cut(s) 646, 673
BfuI GTATCC 1 cut(s) 947
BglI GCCNNNNNGGC 1 cut(s) 1420
BlnI CCTAGG 1 cut(s) 605
Bme1390I CCNGG 3 cut(s) 75, 770, 1417
Bme18I GGWCC 4 cut(s) 152, 248, 1027, 1478
BmgT120I GGNCC 4 cut(s) 152, 248, 1027, 1478
BmiI GGNNCC 2 cut(s) 561, 1446
BmrFI CCNGG 3 cut(s) 75, 770, 1417
BmsI GCATC 2 cut(s) 52, 362
BpmI CTGGAG 2 cut(s) 859, 1438
Bpu10I CCTNAGC 1 cut(s) 549
Bpu14I TTCGAA 2 cut(s) 291, 531
BpuEI CTTGAG 3 cut(s) 64, 470, 1267
BsaHI GRCGYC 2 cut(s) 324, 1275
BsaI GGTCTC 2 cut(s) 148, 1051
BsaJI CCNNGG 6 cut(s) 73, 163, 452, 605, 1415, 1474
BsaWI WCCGGW 3 cut(s) 16, 1024, 1522
BsaXI ACNNNNNCTCC 2 cut(s) 679, 709
Bsc4I CCNNNNNNNGG 5 cut(s) 169, 204, 453, 606, 1401
Bse1I ACTGG 2 cut(s) 842, 1039
Bse3DI GCAATG 1 cut(s) 909
BseBI CCWGG 3 cut(s) 75, 770, 1417
BseDI CCNNGG 6 cut(s) 73, 163, 452, 605, 1415, 1474
BseGI GGATG 4 cut(s) 377, 1021, 1134, 1505
BseLI CCNNNNNNNGG 5 cut(s) 169, 204, 453, 606, 1401
BseMI GCAATG 1 cut(s) 909
BseMII CTCAG 2 cut(s) 540, 1046
BseNI ACTGG 2 cut(s) 842, 1039
BseRI GAGGAG 2 cut(s) 123, 473
BseYI CCCAGC 1 cut(s) 748
Bsh1285I CGRYCG 1 cut(s) 129
BshFI GGCC 3 cut(s) 773, 1423, 1529
BshNI GGYRCC 1 cut(s) 1444
BsiEI CGRYCG 1 cut(s) 129
BsiSI CCGG 4 cut(s) 17, 739, 1025, 1523
BslFI GGGAC 2 cut(s) 101, 669
BslI CCNNNNNNNGG 5 cut(s) 169, 204, 453, 606, 1401
BsmAI GTCTC 4 cut(s) 148, 232, 1051, 1266
BsmBI CGTCTC 1 cut(s) 1266
BsmFI GGGAC 2 cut(s) 101, 669
BsmI GAATGC 3 cut(s) 65, 308, 1280
BsnI GGCC 3 cut(s) 773, 1423, 1529
Bso31I GGTCTC 2 cut(s) 148, 1051
Bsp119I TTCGAA 2 cut(s) 291, 531
Bsp1286I GDGCHC 1 cut(s) 1416
Bsp143I GATC 7 cut(s) 126, 253, 559, 744, 811, 1107, 1257
Bsp19I CCATGG 2 cut(s) 452, 1474
BspACI CCGC 4 cut(s) 56, 246, 791, 955
BspANI GGCC 3 cut(s) 773, 1423, 1529
BspCNI CTCAG 2 cut(s) 541, 1047
BspLI GGNNCC 2 cut(s) 561, 1446
BspPI GGATC 3 cut(s) 554, 567, 739
BspT104I TTCGAA 2 cut(s) 291, 531
BspT107I GGYRCC 1 cut(s) 1444
BspTNI GGTCTC 2 cut(s) 148, 1051
BsrBI CCGCTC 1 cut(s) 793
BsrDI GCAATG 1 cut(s) 909
BsrI ACTGG 2 cut(s) 842, 1039
BssECI CCNNGG 6 cut(s) 73, 163, 452, 605, 1415, 1474
BssMI GATC 7 cut(s) 126, 253, 559, 744, 811, 1107, 1257
BssNI GRCGYC 2 cut(s) 324, 1275
BssT1I CCWWGG 3 cut(s) 452, 605, 1474
Bst2UI CCWGG 3 cut(s) 75, 770, 1417
Bst4CI ACNGT 4 cut(s) 765, 1171, 1217, 1536
Bst6I CTCTTC 1 cut(s) 1005
BstACI GRCGYC 2 cut(s) 324, 1275
BstBI TTCGAA 2 cut(s) 291, 531
BstDEI CTNAG 5 cut(s) 260, 549, 830, 1055, 1102
BstDSI CCRYGG 3 cut(s) 163, 452, 1474
BstENI CCTNNNNNAGG 1 cut(s) 604
BstF5I GGATG 4 cut(s) 377, 1021, 1134, 1505
BstKTI GATC 7 cut(s) 129, 256, 562, 747, 814, 1110, 1260
BstMAI GTCTC 4 cut(s) 148, 232, 1051, 1266
BstMBI GATC 7 cut(s) 126, 253, 559, 744, 811, 1107, 1257
BstMCI CGRYCG 1 cut(s) 129
BstMWI GCNNNNNNNGC 5 cut(s) 62, 84, 575, 790, 1420
BstNI CCWGG 3 cut(s) 75, 770, 1417
BstNSI RCATGY 1 cut(s) 334
BstSCI CCNGG 3 cut(s) 73, 768, 1415
BstSFI CTRYAG 2 cut(s) 646, 673
BstX2I RGATCY 1 cut(s) 559
BstYI RGATCY 1 cut(s) 559
BsuI GTATCC 1 cut(s) 947
BsuRI GGCC 3 cut(s) 773, 1423, 1529
BtgI CCRYGG 3 cut(s) 163, 452, 1474
BtsCI GGATG 4 cut(s) 377, 1021, 1134, 1505
BtsI GCAGTG 1 cut(s) 543
BtsIMutI CAGTG 1 cut(s) 543
CaiI CAGNNNCTG 1 cut(s) 681
Cfr13I GGNCC 4 cut(s) 152, 248, 1027, 1478
Csp6I GTAC 4 cut(s) 281, 835, 1204, 1445
CviAII CATG 5 cut(s) 242, 331, 453, 611, 1475
CviQI GTAC 4 cut(s) 281, 835, 1204, 1445
DdeI CTNAG 5 cut(s) 260, 549, 830, 1055, 1102
DpnI GATC 7 cut(s) 128, 255, 561, 746, 813, 1109, 1259
DpnII GATC 7 cut(s) 126, 253, 559, 744, 811, 1107, 1257
DrdI GACNNNNNNGTC 1 cut(s) 255
DseDI GACNNNNNNGTC 1 cut(s) 255
Eam1104I CTCTTC 1 cut(s) 1005
EarI CTCTTC 1 cut(s) 1005
EciI GGCGGA 1 cut(s) 261
Eco130I CCWWGG 3 cut(s) 452, 605, 1474
Eco147I AGGCCT 1 cut(s) 1423
Eco24I GRGCYC 1 cut(s) 1416
Eco31I GGTCTC 2 cut(s) 148, 1051
Eco47I GGWCC 4 cut(s) 152, 248, 1027, 1478
Eco57I CTGAAG 2 cut(s) 1029, 1314
EcoNI CCTNNNNNAGG 1 cut(s) 604
EcoRI GAATTC 1 cut(s) 1405
EcoRII CCWGG 3 cut(s) 73, 768, 1415
EcoT14I CCWWGG 3 cut(s) 452, 605, 1474
EcoT22I ATGCAT 1 cut(s) 67
EcoT38I GRGCYC 1 cut(s) 1416
ErhI CCWWGG 3 cut(s) 452, 605, 1474
Esp3I CGTCTC 1 cut(s) 1266
FaeI CATG 5 cut(s) 245, 334, 456, 614, 1478
FaqI GGGAC 2 cut(s) 101, 669
FatI CATG 5 cut(s) 241, 330, 452, 610, 1474
FbaI TGATCA 1 cut(s) 1257
FblI GTMKAC 3 cut(s) 13, 826, 1043
FokI GGATG 4 cut(s) 384, 1008, 1121, 1492
FriOI GRGCYC 1 cut(s) 1416
FspBI CTAG 7 cut(s) 474, 566, 606, 632, 1187, 1269, 1308
GsaI CCCAGC 1 cut(s) 752
GsuI CTGGAG 2 cut(s) 859, 1438
HaeIII GGCC 3 cut(s) 773, 1423, 1529
HapII CCGG 4 cut(s) 17, 739, 1025, 1523
Hin1I GRCGYC 2 cut(s) 324, 1275
Hin1II CATG 5 cut(s) 245, 334, 456, 614, 1478
HindIII AAGCTT 1 cut(s) 488
HinfI GANTC 4 cut(s) 219, 460, 819, 924
HpaII CCGG 4 cut(s) 17, 739, 1025, 1523
HphI GGTGA 5 cut(s) 806, 1153, 1219, 1257, 1512
Hpy166II GTNNAC 4 cut(s) 14, 328, 827, 1044
Hpy188I TCNGA 5 cut(s) 152, 1009, 1118, 1257, 1411
Hpy188III TCNNGA 3 cut(s) 81, 228, 1246
Hpy8I GTNNAC 4 cut(s) 14, 328, 827, 1044
Hpy99I CGWCG 1 cut(s) 1280
HpyAV CCTTC 5 cut(s) 784, 1252, 1289, 1491, 1519
HpyCH4III ACNGT 4 cut(s) 765, 1171, 1217, 1536
HpyCH4IV ACGT 2 cut(s) 324, 1275
HpyCH4V TGCA 3 cut(s) 65, 914, 977
HpyF10VI GCNNNNNNNGC 5 cut(s) 62, 84, 575, 790, 1420
HpyF3I CTNAG 5 cut(s) 260, 549, 830, 1055, 1102
HpySE526I ACGT 2 cut(s) 324, 1275
Hsp92I GRCGYC 2 cut(s) 324, 1275
Hsp92II CATG 5 cut(s) 245, 334, 456, 614, 1478
KpnI GGTACC 1 cut(s) 1448
Ksp22I TGATCA 1 cut(s) 1257
Kzo9I GATC 7 cut(s) 126, 253, 559, 744, 811, 1107, 1257
LmnI GCTCC 8 cut(s) 136, 200, 789, 798, 840, 873, 906, 960
LweI GCATC 2 cut(s) 52, 362
MaeI CTAG 7 cut(s) 474, 566, 606, 632, 1187, 1269, 1308
MaeII ACGT 2 cut(s) 324, 1275
MaeIII GTNAC 6 cut(s) 174, 476, 535, 729, 1217, 1310
MalI GATC 7 cut(s) 128, 255, 561, 746, 813, 1109, 1259
MbiI CCGCTC 1 cut(s) 793
MboI GATC 7 cut(s) 126, 253, 559, 744, 811, 1107, 1257
MboII GAAGA 7 cut(s) 71, 425, 502, 533, 580, 821, 1022
MflI RGATCY 1 cut(s) 559
MhlI GDGCHC 1 cut(s) 1416
MluCI AATT 9 cut(s) 185, 287, 465, 524, 915, 1111, 1261, 1352, 1405
MlyI GAGTC 3 cut(s) 213, 828, 918
Mph1103I ATGCAT 1 cut(s) 67
MroXI GAANNNNTTC 1 cut(s) 587
MseI TTAA 6 cut(s) 35, 213, 624, 866, 920, 1077
MspA1I CMGCKG 2 cut(s) 181, 727
MspI CCGG 4 cut(s) 17, 739, 1025, 1523
MspR9I CCNGG 3 cut(s) 75, 770, 1417
Mva1269I GAATGC 3 cut(s) 65, 308, 1280
MvaI CCWGG 3 cut(s) 75, 770, 1417
MwoI GCNNNNNNNGC 5 cut(s) 62, 84, 575, 790, 1420
NcoI CCATGG 2 cut(s) 452, 1474
NdeII GATC 7 cut(s) 126, 253, 559, 744, 811, 1107, 1257
NlaIII CATG 5 cut(s) 245, 334, 456, 614, 1478
NlaIV GGNNCC 2 cut(s) 561, 1446
NmuCI GTSAC 2 cut(s) 476, 729
NsiI ATGCAT 1 cut(s) 67
NspI RCATGY 1 cut(s) 334
NspV TTCGAA 2 cut(s) 291, 531
PceI AGGCCT 1 cut(s) 1423
PciI ACATGT 1 cut(s) 330
PctI GAATGC 3 cut(s) 65, 308, 1280
PdmI GAANNNNTTC 1 cut(s) 587
PfeI GAWTC 1 cut(s) 460
PflMI CCANNNNNTGG 2 cut(s) 204, 453
Ple19I CGATCG 1 cut(s) 129
PleI GAGTC 3 cut(s) 213, 827, 918
PpsI GAGTC 3 cut(s) 213, 827, 918
PscI ACATGT 1 cut(s) 330
Psp6I CCWGG 3 cut(s) 73, 768, 1415
PspFI CCCAGC 1 cut(s) 748
PspGI CCWGG 3 cut(s) 73, 768, 1415
PspN4I GGNNCC 2 cut(s) 561, 1446
PspPI GGNCC 4 cut(s) 152, 248, 1027, 1478
PstNI CAGNNNCTG 1 cut(s) 681
PsuI RGATCY 1 cut(s) 559
PvuI CGATCG 1 cut(s) 129
PvuII CAGCTG 2 cut(s) 181, 727
RgaI GCGATCGC 1 cut(s) 129
RsaI GTAC 4 cut(s) 282, 836, 1205, 1446
RsaNI GTAC 4 cut(s) 281, 835, 1204, 1445
SaqAI TTAA 6 cut(s) 35, 213, 624, 866, 920, 1077
Sau3AI GATC 7 cut(s) 126, 253, 559, 744, 811, 1107, 1257
Sau96I GGNCC 4 cut(s) 152, 248, 1027, 1478
SchI GAGTC 3 cut(s) 213, 828, 918
ScrFI CCNGG 3 cut(s) 75, 770, 1417
SduI GDGCHC 1 cut(s) 1416
SfaAI GCGATCGC 1 cut(s) 129
SfaNI GCATC 2 cut(s) 52, 362
SfcI CTRYAG 2 cut(s) 646, 673
SfuI TTCGAA 2 cut(s) 291, 531
SgfI GCGATCGC 1 cut(s) 129
SinI GGWCC 4 cut(s) 152, 248, 1027, 1478
SmlI CTYRAG 3 cut(s) 79, 485, 1246
SmoI CTYRAG 3 cut(s) 79, 485, 1246
SpeI ACTAGT 1 cut(s) 473
Sse9I AATT 9 cut(s) 185, 287, 465, 524, 915, 1111, 1261, 1352, 1405
SseBI AGGCCT 1 cut(s) 1423
SsiI CCGC 4 cut(s) 56, 246, 791, 955
SspMI CTAG 7 cut(s) 474, 566, 606, 632, 1187, 1269, 1308
StuI AGGCCT 1 cut(s) 1423
StyD4I CCNGG 3 cut(s) 73, 768, 1415
StyI CCWWGG 3 cut(s) 452, 605, 1474
TaaI ACNGT 4 cut(s) 765, 1171, 1217, 1536
TaiI ACGT 2 cut(s) 327, 1278
TaqI TCGA 8 cut(s) 217, 229, 291, 351, 394, 429, 531, 703
TasI AATT 9 cut(s) 185, 287, 465, 524, 915, 1111, 1261, 1352, 1405
TfiI GAWTC 1 cut(s) 460
Tru1I TTAA 6 cut(s) 35, 213, 624, 866, 920, 1077
Tru9I TTAA 6 cut(s) 35, 213, 624, 866, 920, 1077
TscAI CASTG 1 cut(s) 550
TseFI GTSAC 2 cut(s) 476, 729
Tsp45I GTSAC 2 cut(s) 476, 729
TspDTI ATGAA 5 cut(s) 53, 426, 630, 1209, 1473
TspGWI ACGGA 1 cut(s) 180
TspRI CASTG 1 cut(s) 550
Van91I CCANNNNNTGG 2 cut(s) 204, 453
VpaK11BI GGWCC 4 cut(s) 152, 248, 1027, 1478
XagI CCTNNNNNAGG 1 cut(s) 604
XapI RAATTY 3 cut(s) 185, 287, 1405
XceI RCATGY 1 cut(s) 334
XmaJI CCTAGG 1 cut(s) 605
XmiI GTMKAC 3 cut(s) 13, 826, 1043
XmnI GAANNNNTTC 1 cut(s) 587
XspI CTAG 7 cut(s) 474, 566, 606, 632, 1187, 1269, 1308
ZraI GACGTC 2 cut(s) 325, 1276
Zsp2I ATGCAT 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.