pycom12g02510

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002694
Physical Location & Seq
Forward (+)
2185433 .. 2186806
1374 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g02510.1

Sequence Viewer

Length: 1374 bp
ATGGATTATAGTGGGGCTATCCAAGTGGTAGCAGCGCAAAATTTTACTGCAGAAGAAGTCGTGATTGATATCGACGAATCTGCAGTTGATATGCAAAGCCGTCTTGAACAAAACAGACGAGAAATTCCAACAACAAAAAATAGGAGCTCGGTGTGCATATATAGAGTTCCTCCCGGCCTCGCCGGAATCAGTCCAAAAGCCATTCAGCCAGAGAGAGTCTCCATCGGGCCATACCACCACGGCAAAAAGGAGTTATCGGAATTTCAGAGATACAAATGGTGGTTTCTTGATCGCCTCCTTTGGCGAACAAAGCGCGGCCTACAAGACTACGTGAAAGCCATGAGCAGGTTGGAGAAAAGCACGAGAAATTGCTATTCCGACCACCACGAGTTGAGCGTCAGCGACTTTGTTGAAATGATGGTTCTTGATGGGTGCTTTATAATTGAGCTTTTTCGATTTGTTTGTGAGAGCAATGACGATGATCACGTCGTTGATGAAAATGATCGCGAGGGCAACCCGATCTTAGCCACGCCATGGTTGATTCCAATTCTCACGAGGGACCTCCTCAAGCTTGAAAATCAACTCCCTTTCTCTGTCCTCGAAACATTATACGACATCTCGAGGGATCAACGTAAAGCCGCGGTTCCACTAGTCATTCGAGCGGTGAAGTTCTTCAGTAATTCATTACTTAGGCCTACTGAGGTCCTTAAAAACTCAAGAATGTTGGAGCCTGAGCACTTGCTTGACTTGTTTCGCTTAACCTTCCTTCCCCTCAAAGGTACTTCAAATGATCAGTACTCTTCTCAAAAGTTATACCGTCCATCCTCCGAGTCAATCCAGTGTACCACAGAACTACGATCCTCGGGGATCGAGTTCAAGGCACGCAAAGCAGCGGACAGCTTCCTTGACATCAATTTCCAAAACAGGGTTCTATATATCCCACCTTTAACCATCAATGACTTCACCACTACTTTCGTCATCAATTGCTTGGCCTTAGAACAATGCCGCCAATACAGTACAGGTACGTGGTTCAGCACTTACATTACTTTCATGAGCTGTCTCCTCACTTCAACTAGAGACGTCAAGTTACTGTGTTCTAATGGGATCATCAGCACCGGCTTCTCACAGAATGACCAAAACGTAGCTGAGATGTTTAGAAAGTTGGGGGAAAAAGCTGGTTTCAACATAAGAGATTGCTACCTCTCCAAGCAATTCAGAGATGTGGAGGCCTATTACAGCAGCCATTGGGCTACTTTCATGCGCACCTATTTTAGCAAGCAATGGTCCTTCATTACAGTTTTTTACGCCTTCATCCTTCTTGTCGTTACCGGAGGCCAAACTGCTATGGCTATCATGAGCTACATCAACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

458

Amino Acids

52.8

Weight (kDa)

6.68

Isoelectric Point (pI)

38.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 53 - 441 1.3e-108 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 440
AatII GACGTC 1 cut(s) 1083
Acc16I TGCGCA 1 cut(s) 1262
Acc36I ACCTGC 1 cut(s) 336
AccB7I CCANNNNNTGG 1 cut(s) 534
AccBSI CCGCTC 1 cut(s) 662
AccII CGCG 3 cut(s) 315, 507, 641
AciI CCGC 6 cut(s) 315, 639, 641, 662, 893, 1006
AclWI GGATC 4 cut(s) 633, 852, 875, 1112
AcsI RAATTY 3 cut(s) 40, 123, 260
AcuI CTGAAG 1 cut(s) 658
AcyI GRCGYC 1 cut(s) 1080
AfaI GTAC 5 cut(s) 781, 797, 844, 1018, 1024
AfiI CCNNNNNNNGG 4 cut(s) 345, 534, 776, 925
AgsI TTSAA 7 cut(s) 107, 413, 575, 786, 877, 1071, 1183
AhlI ACTAGT 1 cut(s) 649
AjiI CACGTC 1 cut(s) 487
AjuI GAANNNNNNNTTGG 2 cut(s) 912, 944
AluBI AGCT 8 cut(s) 147, 448, 571, 900, 1056, 1145, 1175, 1359
AluI AGCT 8 cut(s) 147, 448, 571, 900, 1056, 1145, 1175, 1359
Alw21I GWGCWC 2 cut(s) 149, 738
Alw26I GTCTC 3 cut(s) 223, 1064, 1071
AlwI GGATC 4 cut(s) 633, 852, 875, 1112
Ama87I CYCGRG 2 cut(s) 619, 862
AoxI GGCC 7 cut(s) 175, 227, 316, 692, 990, 1227, 1333
ApeKI GCWGC 3 cut(s) 32, 890, 1239
ApoI RAATTY 3 cut(s) 40, 123, 260
Asp700I GAANNNNTTC 1 cut(s) 671
AspLEI GCGC 3 cut(s) 37, 315, 1263
AspS9I GGNCC 4 cut(s) 227, 559, 703, 1284
AsuC2I CCSGG 1 cut(s) 174
AsuHPI GGTGA 2 cut(s) 676, 955
AvaI CYCGRG 2 cut(s) 619, 862
AvaII GGWCC 3 cut(s) 559, 703, 1284
BanII GRGCYC 1 cut(s) 149
BauI CACGAG 3 cut(s) 361, 386, 553
Bbv12I GWGCWC 2 cut(s) 149, 738
BbvI GCAGC 3 cut(s) 44, 902, 1251
BccI CCATC 5 cut(s) 230, 412, 422, 829, 959
BceAI ACGGC 2 cut(s) 84, 256
BcgI CGANNNNNNTGC 2 cut(s) 62, 96
BclI TGATCA 2 cut(s) 481, 790
BcnI CCSGG 1 cut(s) 174
BcoDI GTCTC 3 cut(s) 223, 1064, 1071
BcuI ACTAGT 1 cut(s) 649
BfaI CTAG 2 cut(s) 650, 1074
BfmI CTRYAG 2 cut(s) 48, 81
BfuAI ACCTGC 1 cut(s) 336
BisI GCNGC 6 cut(s) 33, 316, 639, 891, 1006, 1240
BlsI GCNGC 6 cut(s) 34, 317, 640, 892, 1007, 1241
BmcAI AGTACT 1 cut(s) 797
Bme1390I CCNGG 1 cut(s) 174
Bme18I GGWCC 3 cut(s) 559, 703, 1284
BmeT110I CYCGRG 2 cut(s) 619, 862
BmgBI CACGTC 1 cut(s) 487
BmgT120I GGNCC 4 cut(s) 227, 559, 703, 1284
BmiI GGNNCC 3 cut(s) 560, 645, 729
BmrFI CCNGG 1 cut(s) 174
BplI GAGNNNNNCTC 2 cut(s) 203, 235
Bpu10I CCTNAGC 1 cut(s) 732
BpuEI CTTGAG 2 cut(s) 551, 700
BpuMI CCSGG 1 cut(s) 174
BsaAI YACGTR 2 cut(s) 331, 1026
BsaBI GATNNNNATC 1 cut(s) 68
BsaHI GRCGYC 1 cut(s) 1080
BsaJI CCNNGG 4 cut(s) 238, 533, 639, 861
BsaWI WCCGGW 1 cut(s) 1328
Bsc4I CCNNNNNNNGG 4 cut(s) 345, 534, 776, 925
Bse118I RCCGGY 1 cut(s) 1115
Bse1I ACTGG 1 cut(s) 838
Bse3DI GCAATG 2 cut(s) 478, 1286
Bse8I GATNNNNATC 1 cut(s) 68
BseDI CCNNGG 4 cut(s) 238, 533, 639, 861
BseGI GGATG 2 cut(s) 821, 1311
BseJI GATNNNNATC 1 cut(s) 68
BseLI CCNNNNNNNGG 4 cut(s) 345, 534, 776, 925
BseMI GCAATG 2 cut(s) 478, 1286
BseMII CTCAG 3 cut(s) 690, 723, 1137
BseNI ACTGG 1 cut(s) 838
BseRI GAGGAG 2 cut(s) 554, 1052
BseXI GCAGC 3 cut(s) 44, 902, 1251
Bsh1236I CGCG 3 cut(s) 315, 507, 641
BshFI GGCC 7 cut(s) 177, 229, 318, 694, 992, 1229, 1335
BsiHKAI GWGCWC 2 cut(s) 149, 738
BsiHKCI CYCGRG 2 cut(s) 619, 862
BsiSI CCGG 4 cut(s) 174, 183, 1116, 1329
BslFI GGGAC 1 cut(s) 572
BslI CCNNNNNNNGG 4 cut(s) 345, 534, 776, 925
BsmAI GTCTC 3 cut(s) 223, 1064, 1071
BsmBI CGTCTC 1 cut(s) 1071
BsmFI GGGAC 1 cut(s) 572
BsnI GGCC 7 cut(s) 177, 229, 318, 694, 992, 1229, 1335
BsoBI CYCGRG 2 cut(s) 619, 862
Bsp1286I GDGCHC 2 cut(s) 149, 738
Bsp143I GATC 9 cut(s) 289, 481, 502, 519, 625, 790, 857, 867, 1104
Bsp19I CCATGG 1 cut(s) 533
Bsp68I TCGCGA 1 cut(s) 507
BspACI CCGC 6 cut(s) 315, 639, 641, 662, 893, 1006
BspANI GGCC 7 cut(s) 177, 229, 318, 694, 992, 1229, 1335
BspCNI CTCAG 3 cut(s) 691, 724, 1138
BspFNI CGCG 3 cut(s) 315, 507, 641
BspHI TCATGA 2 cut(s) 1050, 1353
BspLI GGNNCC 3 cut(s) 560, 645, 729
BspMAI CTGCAG 2 cut(s) 52, 85
BspMI ACCTGC 1 cut(s) 336
BspPI GGATC 4 cut(s) 633, 852, 875, 1112
BsrBI CCGCTC 1 cut(s) 662
BsrDI GCAATG 2 cut(s) 478, 1286
BsrFI RCCGGY 1 cut(s) 1115
BsrI ACTGG 1 cut(s) 838
BssAI RCCGGY 1 cut(s) 1115
BssECI CCNNGG 4 cut(s) 238, 533, 639, 861
BssMI GATC 9 cut(s) 289, 481, 502, 519, 625, 790, 857, 867, 1104
BssNI GRCGYC 1 cut(s) 1080
BssSI CACGAG 3 cut(s) 361, 386, 553
BssT1I CCWWGG 1 cut(s) 533
Bst2BI CACGAG 3 cut(s) 361, 386, 553
Bst4CI ACNGT 4 cut(s) 818, 1016, 1092, 1297
Bst6I CTCTTC 1 cut(s) 805
BstACI GRCGYC 1 cut(s) 1080
BstBAI YACGTR 2 cut(s) 331, 1026
BstC8I GCNNGC 2 cut(s) 883, 1277
BstDEI CTNAG 6 cut(s) 523, 689, 699, 732, 994, 1146
BstDSI CCRYGG 3 cut(s) 238, 533, 639
BstF5I GGATG 2 cut(s) 821, 1311
BstFNI CGCG 3 cut(s) 315, 507, 641
BstHHI GCGC 3 cut(s) 37, 315, 1263
BstKTI GATC 9 cut(s) 292, 484, 505, 522, 628, 793, 860, 870, 1107
BstMAI GTCTC 3 cut(s) 223, 1064, 1071
BstMBI GATC 9 cut(s) 289, 481, 502, 519, 625, 790, 857, 867, 1104
BstMWI GCNNNNNNNGC 3 cut(s) 153, 310, 887
BstSCI CCNGG 1 cut(s) 172
BstSFI CTRYAG 2 cut(s) 48, 81
BstUI CGCG 3 cut(s) 315, 507, 641
BstV1I GCAGC 3 cut(s) 44, 902, 1251
BsuRI GGCC 7 cut(s) 177, 229, 318, 694, 992, 1229, 1335
BtgI CCRYGG 3 cut(s) 238, 533, 639
BtrI CACGTC 1 cut(s) 487
BtsCI GGATG 2 cut(s) 821, 1311
BtsIMutI CAGTG 1 cut(s) 845
BtuMI TCGCGA 1 cut(s) 507
BveI ACCTGC 1 cut(s) 336
Cac8I GCNNGC 2 cut(s) 883, 1277
CciI TCATGA 2 cut(s) 1050, 1353
CfoI GCGC 3 cut(s) 37, 315, 1263
Cfr10I RCCGGY 1 cut(s) 1115
Cfr13I GGNCC 4 cut(s) 227, 559, 703, 1284
Cfr42I CCGCGG 1 cut(s) 642
CseI GACGC 1 cut(s) 385
Csp6I GTAC 5 cut(s) 780, 796, 843, 1017, 1023
CviAII CATG 5 cut(s) 340, 534, 1051, 1258, 1354
CviQI GTAC 5 cut(s) 780, 796, 843, 1017, 1023
DdeI CTNAG 6 cut(s) 523, 689, 699, 732, 994, 1146
DpnI GATC 9 cut(s) 291, 483, 504, 521, 627, 792, 859, 869, 1106
DpnII GATC 9 cut(s) 289, 481, 502, 519, 625, 790, 857, 867, 1104
Eam1104I CTCTTC 1 cut(s) 805
EarI CTCTTC 1 cut(s) 805
Ecl136II GAGCTC 1 cut(s) 147
Eco130I CCWWGG 1 cut(s) 533
Eco147I AGGCCT 2 cut(s) 694, 1229
Eco24I GRGCYC 1 cut(s) 149
Eco32I GATATC 1 cut(s) 70
Eco47I GGWCC 3 cut(s) 559, 703, 1284
Eco53kI GAGCTC 1 cut(s) 147
Eco57I CTGAAG 1 cut(s) 658
Eco88I CYCGRG 2 cut(s) 619, 862
EcoICRI GAGCTC 1 cut(s) 147
EcoO109I RGGNCCY 2 cut(s) 559, 703
EcoRV GATATC 1 cut(s) 70
EcoT14I CCWWGG 1 cut(s) 533
EcoT38I GRGCYC 1 cut(s) 149
ErhI CCWWGG 1 cut(s) 533
Esp3I CGTCTC 1 cut(s) 1071
FaeI CATG 5 cut(s) 343, 537, 1054, 1261, 1357
FaqI GGGAC 1 cut(s) 572
FatI CATG 5 cut(s) 339, 533, 1050, 1257, 1353
FbaI TGATCA 2 cut(s) 481, 790
Fnu4HI GCNGC 6 cut(s) 33, 316, 639, 891, 1006, 1240
FokI GGATG 2 cut(s) 808, 1298
FriOI GRGCYC 1 cut(s) 149
Fsp4HI GCNGC 6 cut(s) 33, 316, 639, 891, 1006, 1240
FspAI RTGCGCAY 1 cut(s) 1262
FspBI CTAG 2 cut(s) 650, 1074
FspI TGCGCA 1 cut(s) 1262
GlaI GCGC 3 cut(s) 36, 314, 1262
GluI GCNGC 6 cut(s) 33, 316, 639, 891, 1006, 1240
HaeIII GGCC 7 cut(s) 177, 229, 318, 694, 992, 1229, 1335
HapII CCGG 4 cut(s) 174, 183, 1116, 1329
HgaI GACGC 1 cut(s) 385
HhaI GCGC 3 cut(s) 37, 315, 1263
Hin1I GRCGYC 1 cut(s) 1080
Hin1II CATG 5 cut(s) 343, 537, 1054, 1261, 1357
Hin6I GCGC 3 cut(s) 35, 313, 1261
HinP1I GCGC 3 cut(s) 35, 313, 1261
HindIII AAGCTT 1 cut(s) 569
HinfI GANTC 5 cut(s) 77, 186, 216, 541, 830
HpaII CCGG 4 cut(s) 174, 183, 1116, 1329
HphI GGTGA 2 cut(s) 676, 955
Hpy166II GTNNAC 1 cut(s) 843
Hpy188I TCNGA 5 cut(s) 259, 267, 379, 829, 1217
Hpy8I GTNNAC 1 cut(s) 843
Hpy99I CGWCG 2 cut(s) 77, 491
HpyAV CCTTC 5 cut(s) 772, 776, 1297, 1318, 1325
HpyCH4III ACNGT 4 cut(s) 818, 1016, 1092, 1297
HpyCH4IV ACGT 6 cut(s) 330, 486, 631, 1025, 1080, 1140
HpyCH4V TGCA 4 cut(s) 50, 83, 94, 156
HpyF10VI GCNNNNNNNGC 3 cut(s) 153, 310, 887
HpyF3I CTNAG 6 cut(s) 523, 689, 699, 732, 994, 1146
HpySE526I ACGT 6 cut(s) 330, 486, 631, 1025, 1080, 1140
Hsp92I GRCGYC 1 cut(s) 1080
Hsp92II CATG 5 cut(s) 343, 537, 1054, 1261, 1357
HspAI GCGC 3 cut(s) 35, 313, 1261
Ksp22I TGATCA 2 cut(s) 481, 790
KspI CCGCGG 1 cut(s) 642
Kzo9I GATC 9 cut(s) 289, 481, 502, 519, 625, 790, 857, 867, 1104
LmnI GCTCC 2 cut(s) 144, 727
Lsp1109I GCAGC 3 cut(s) 44, 902, 1251
MaeI CTAG 2 cut(s) 650, 1074
MaeII ACGT 6 cut(s) 330, 486, 631, 1025, 1080, 1140
MaeIII GTNAC 2 cut(s) 1086, 1324
MalI GATC 9 cut(s) 291, 483, 504, 521, 627, 792, 859, 869, 1106
MbiI CCGCTC 1 cut(s) 662
MboI GATC 9 cut(s) 289, 481, 502, 519, 625, 790, 857, 867, 1104
MboII GAAGA 3 cut(s) 65, 664, 792
MfeI CAATTG 1 cut(s) 982
MhlI GDGCHC 2 cut(s) 149, 738
MlyI GAGTC 2 cut(s) 225, 839
MmeI TCCRAC 4 cut(s) 152, 330, 402, 705
MroXI GAANNNNTTC 1 cut(s) 671
MseI TTAA 4 cut(s) 708, 758, 947, 1372
MspA1I CMGCKG 2 cut(s) 641, 893
MspI CCGG 4 cut(s) 174, 183, 1116, 1329
MspR9I CCNGG 1 cut(s) 174
MunI CAATTG 1 cut(s) 982
MvnI CGCG 3 cut(s) 315, 507, 641
MwoI GCNNNNNNNGC 3 cut(s) 153, 310, 887
NciI CCSGG 1 cut(s) 174
NcoI CCATGG 1 cut(s) 533
NdeII GATC 9 cut(s) 289, 481, 502, 519, 625, 790, 857, 867, 1104
NlaIII CATG 5 cut(s) 343, 537, 1054, 1261, 1357
NlaIV GGNNCC 3 cut(s) 560, 645, 729
NruI TCGCGA 1 cut(s) 507
NsbI TGCGCA 1 cut(s) 1262
PaeR7I CTCGAG 1 cut(s) 619
PagI TCATGA 2 cut(s) 1050, 1353
PceI AGGCCT 2 cut(s) 694, 1229
PcsI WCGNNNNNNNCGW 2 cut(s) 393, 483
PdmI GAANNNNTTC 1 cut(s) 671
PfeI GAWTC 3 cut(s) 77, 186, 541
PflMI CCANNNNNTGG 1 cut(s) 534
PkrI GCNGC 6 cut(s) 34, 317, 640, 892, 1007, 1241
PleI GAGTC 2 cut(s) 224, 838
PpsI GAGTC 2 cut(s) 224, 838
Ppu21I YACGTR 2 cut(s) 331, 1026
PpuMI RGGWCCY 2 cut(s) 559, 703
PsiI TTATAA 1 cut(s) 440
Psp124BI GAGCTC 1 cut(s) 149
Psp5II RGGWCCY 2 cut(s) 559, 703
PspN4I GGNNCC 3 cut(s) 560, 645, 729
PspPI GGNCC 4 cut(s) 227, 559, 703, 1284
PspPPI RGGWCCY 2 cut(s) 559, 703
PstI CTGCAG 2 cut(s) 52, 85
RruI TCGCGA 1 cut(s) 507
RsaI GTAC 5 cut(s) 781, 797, 844, 1018, 1024
RsaNI GTAC 5 cut(s) 780, 796, 843, 1017, 1023
SacI GAGCTC 1 cut(s) 149
SacII CCGCGG 1 cut(s) 642
SaqAI TTAA 4 cut(s) 708, 758, 947, 1372
SatI GCNGC 6 cut(s) 33, 316, 639, 891, 1006, 1240
Sau3AI GATC 9 cut(s) 289, 481, 502, 519, 625, 790, 857, 867, 1104
Sau96I GGNCC 4 cut(s) 227, 559, 703, 1284
ScaI AGTACT 1 cut(s) 797
SchI GAGTC 2 cut(s) 225, 839
ScrFI CCNGG 1 cut(s) 174
SduI GDGCHC 2 cut(s) 149, 738
SfcI CTRYAG 2 cut(s) 48, 81
Sfr274I CTCGAG 1 cut(s) 619
Sfr303I CCGCGG 1 cut(s) 642
SgrBI CCGCGG 1 cut(s) 642
SinI GGWCC 3 cut(s) 559, 703, 1284
SlaI CTCGAG 1 cut(s) 619
SmlI CTYRAG 3 cut(s) 566, 619, 715
SmoI CTYRAG 3 cut(s) 566, 619, 715
SpeI ACTAGT 1 cut(s) 649
SseBI AGGCCT 2 cut(s) 694, 1229
SsiI CCGC 6 cut(s) 315, 639, 641, 662, 893, 1006
SspMI CTAG 2 cut(s) 650, 1074
SstI GAGCTC 1 cut(s) 149
StuI AGGCCT 2 cut(s) 694, 1229
StyD4I CCNGG 1 cut(s) 172
StyI CCWWGG 1 cut(s) 533
TaaI ACNGT 4 cut(s) 818, 1016, 1092, 1297
TaiI ACGT 6 cut(s) 333, 489, 634, 1028, 1083, 1143
TaqI TCGA 6 cut(s) 72, 454, 600, 620, 658, 870
TatI WGTACW 2 cut(s) 795, 1016
TauI GCSGC 3 cut(s) 318, 641, 1008
TfiI GAWTC 3 cut(s) 77, 186, 541
Tru1I TTAA 4 cut(s) 708, 758, 947, 1372
Tru9I TTAA 4 cut(s) 708, 758, 947, 1372
TscAI CASTG 1 cut(s) 845
TseI GCWGC 3 cut(s) 32, 890, 1239
TspDTI ATGAA 6 cut(s) 510, 672, 1039, 1246, 1279, 1300
TspRI CASTG 1 cut(s) 845
Van91I CCANNNNNTGG 1 cut(s) 534
VpaK11BI GGWCC 3 cut(s) 559, 703, 1284
XapI RAATTY 3 cut(s) 40, 123, 260
XcmI CCANNNNNNNNNTGG 1 cut(s) 346
XhoI CTCGAG 1 cut(s) 619
XmnI GAANNNNTTC 1 cut(s) 671
XspI CTAG 2 cut(s) 650, 1074
ZraI GACGTC 1 cut(s) 1081
ZrmI AGTACT 1 cut(s) 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.