RLG00000036091

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0002694
Physical Location & Seq
Forward (+)
75670874 .. 75671740
867 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036091

Sequence Viewer

Length: 618 bp
ATGAAACAGTTGGAGGACAGAACTAGAGGATGCTATGATGAGGACATCCCCATGTCAAGCTCGGACTTCATTGAAATGATGGTGCTTGATGGGTGCTTGATCATTGAGCTATTTCGACATGTTTGTAGAAATGATGATGTCATAGGTAAAAATGATCCCATCTCAAGCACGCCATGGTTGATTCCCATTCTCACTAGGGACCTCCTCAAGCTCGAAAACCAACTGCCTTTCTTCGTCCTTGAAAGATATTTGATCTCACCAGAACCCCTGGACAGCTTCTTAGAGATCGAGTTCAAAAACAGGGTTCTACATGTCCCACCATTTACCATCAATGACCTCACAATTGCTGTCTTCATCAATTGCATGGCCTTGGAACATTGTAAATATTCCATTCCAAAGTACTTCACTAGTTACATTGCCTTCATGAGCTGCCTCATCAATTCACCTAGAGACGTTGGGTTCCTTTGTGCTGATGGAATTATTACTAGCTACTCGCACAATGATCACTACATAGTTGGGTTGTTCAACAAGTTAGGGGAAAAAGTTTACCTTGACAAAAGTGACTGCTACCTCTCCAAGCAATTCAGAGATGTGGAGGCCTATTACGGCAGCCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

206

Amino Acids

23.74

Weight (kDa)

4.89

Isoelectric Point (pI)

46.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 1 - 83 1.6e-24 Plant protein of unknown function
DUF247 PF03140 91 - 204 2.1e-31 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 149
AfaI GTAC 1 cut(s) 401
AflIII ACRYGT 2 cut(s) 118, 310
AgsI TTSAA 4 cut(s) 74, 242, 295, 526
AhlI ACTAGT 1 cut(s) 407
AjnI CCWGG 1 cut(s) 267
AluBI AGCT 6 cut(s) 60, 109, 211, 276, 429, 489
AluI AGCT 6 cut(s) 60, 109, 211, 276, 429, 489
Alw26I GTCTC 1 cut(s) 444
AlwI GGATC 1 cut(s) 149
AoxI GGCC 2 cut(s) 366, 597
ApeKI GCWGC 2 cut(s) 429, 609
AspS9I GGNCC 1 cut(s) 199
AsuHPI GGTGA 2 cut(s) 249, 435
AvaII GGWCC 1 cut(s) 199
BbsI GAAGAC 1 cut(s) 343
BbvI GCAGC 1 cut(s) 416
BccI CCATC 5 cut(s) 73, 83, 167, 335, 467
BciT130I CCWGG 1 cut(s) 269
BclI TGATCA 2 cut(s) 99, 502
BcoDI GTCTC 1 cut(s) 444
BcuI ACTAGT 1 cut(s) 407
BfaI CTAG 5 cut(s) 24, 195, 408, 447, 486
BisI GCNGC 2 cut(s) 430, 610
BlsI GCNGC 2 cut(s) 431, 611
BmcAI AGTACT 1 cut(s) 401
Bme1390I CCNGG 1 cut(s) 269
Bme18I GGWCC 1 cut(s) 199
BmgT120I GGNCC 1 cut(s) 199
BmiI GGNNCC 2 cut(s) 200, 461
BmrFI CCNGG 1 cut(s) 269
BmsI GCATC 1 cut(s) 20
BpiI GAAGAC 1 cut(s) 343
BpuEI CTTGAG 2 cut(s) 148, 191
BsaJI CCNNGG 3 cut(s) 173, 267, 369
Bse3DI GCAATG 1 cut(s) 414
BseBI CCWGG 1 cut(s) 269
BseDI CCNNGG 3 cut(s) 173, 267, 369
BseGI GGATG 2 cut(s) 35, 45
BseMI GCAATG 1 cut(s) 414
BseRI GAGGAG 1 cut(s) 194
BseXI GCAGC 1 cut(s) 416
BshFI GGCC 2 cut(s) 368, 599
BslFI GGGAC 2 cut(s) 212, 299
BsmAI GTCTC 1 cut(s) 444
BsmBI CGTCTC 1 cut(s) 444
BsmFI GGGAC 2 cut(s) 212, 299
BsnI GGCC 2 cut(s) 368, 599
Bsp143I GATC 5 cut(s) 99, 154, 252, 285, 502
Bsp19I CCATGG 1 cut(s) 173
BspANI GGCC 2 cut(s) 368, 599
BspHI TCATGA 1 cut(s) 423
BspLI GGNNCC 2 cut(s) 200, 461
BspPI GGATC 1 cut(s) 149
BsrDI GCAATG 1 cut(s) 414
BssECI CCNNGG 3 cut(s) 173, 267, 369
BssMI GATC 5 cut(s) 99, 154, 252, 285, 502
BssT1I CCWWGG 2 cut(s) 173, 369
Bst2UI CCWGG 1 cut(s) 269
Bst4CI ACNGT 1 cut(s) 9
BstC8I GCNNGC 1 cut(s) 170
BstDEI CTNAG 2 cut(s) 280, 615
BstDSI CCRYGG 1 cut(s) 173
BstF5I GGATG 2 cut(s) 35, 45
BstKTI GATC 5 cut(s) 102, 157, 255, 288, 505
BstMAI GTCTC 1 cut(s) 444
BstMBI GATC 5 cut(s) 99, 154, 252, 285, 502
BstNI CCWGG 1 cut(s) 269
BstNSI RCATGY 2 cut(s) 122, 314
BstSCI CCNGG 1 cut(s) 267
BstV1I GCAGC 1 cut(s) 416
BstV2I GAAGAC 1 cut(s) 343
BsuRI GGCC 2 cut(s) 368, 599
BtgI CCRYGG 1 cut(s) 173
BtsCI GGATG 2 cut(s) 35, 45
Cac8I GCNNGC 1 cut(s) 170
CciI TCATGA 1 cut(s) 423
Cfr13I GGNCC 1 cut(s) 199
Csp6I GTAC 1 cut(s) 400
CviAII CATG 6 cut(s) 52, 119, 174, 311, 364, 424
CviJI RGCY 9 cut(s) 60, 109, 211, 276, 368, 429, 489, 599, 612
CviKI_1 RGCY 9 cut(s) 60, 109, 211, 276, 368, 429, 489, 599, 612
CviQI GTAC 1 cut(s) 400
DdeI CTNAG 2 cut(s) 280, 615
DpnI GATC 5 cut(s) 101, 156, 254, 287, 504
DpnII GATC 5 cut(s) 99, 154, 252, 285, 502
Eco130I CCWWGG 2 cut(s) 173, 369
Eco147I AGGCCT 1 cut(s) 599
Eco47I GGWCC 1 cut(s) 199
EcoO109I RGGNCCY 1 cut(s) 199
EcoRII CCWGG 1 cut(s) 267
EcoT14I CCWWGG 2 cut(s) 173, 369
ErhI CCWWGG 2 cut(s) 173, 369
Esp3I CGTCTC 1 cut(s) 444
FaeI CATG 6 cut(s) 55, 122, 177, 314, 367, 427
FaiI YATR 9 cut(s) 36, 53, 120, 143, 175, 312, 365, 425, 512
FalI AAGNNNNNCTT 2 cut(s) 534, 566
FaqI GGGAC 2 cut(s) 212, 299
FatI CATG 6 cut(s) 51, 118, 173, 310, 363, 423
FbaI TGATCA 2 cut(s) 99, 502
Fnu4HI GCNGC 2 cut(s) 430, 610
FokI GGATG 2 cut(s) 32, 42
Fsp4HI GCNGC 2 cut(s) 430, 610
FspBI CTAG 5 cut(s) 24, 195, 408, 447, 486
GluI GCNGC 2 cut(s) 430, 610
HaeIII GGCC 2 cut(s) 368, 599
Hin1II CATG 6 cut(s) 55, 122, 177, 314, 367, 427
HinfI GANTC 1 cut(s) 181
HphI GGTGA 2 cut(s) 249, 435
Hpy166II GTNNAC 1 cut(s) 547
Hpy188I TCNGA 2 cut(s) 64, 587
Hpy188III TCNNGA 1 cut(s) 424
Hpy8I GTNNAC 1 cut(s) 547
HpyAV CCTTC 1 cut(s) 430
HpyCH4III ACNGT 1 cut(s) 9
HpyCH4IV ACGT 1 cut(s) 453
HpyCH4V TGCA 1 cut(s) 363
HpyF3I CTNAG 2 cut(s) 280, 615
HpySE526I ACGT 1 cut(s) 453
Hsp92II CATG 6 cut(s) 55, 122, 177, 314, 367, 427
Ksp22I TGATCA 2 cut(s) 99, 502
Kzo9I GATC 5 cut(s) 99, 154, 252, 285, 502
LpnPI CCDG 4 cut(s) 254, 273, 281, 286
Lsp1109I GCAGC 1 cut(s) 416
LweI GCATC 1 cut(s) 20
MaeI CTAG 5 cut(s) 24, 195, 408, 447, 486
MaeII ACGT 1 cut(s) 453
MaeIII GTNAC 2 cut(s) 410, 560
MalI GATC 5 cut(s) 101, 156, 254, 287, 504
MboI GATC 5 cut(s) 99, 154, 252, 285, 502
MboII GAAGA 2 cut(s) 223, 343
MfeI CAATTG 2 cut(s) 342, 358
MluCI AATT 5 cut(s) 342, 358, 439, 477, 581
MnlI CCTC 9 cut(s) 7, 20, 34, 212, 215, 347, 443, 581, 589
MslI CAYNNNNRTG 2 cut(s) 50, 74
MspR9I CCNGG 1 cut(s) 269
MunI CAATTG 2 cut(s) 342, 358
MvaI CCWGG 1 cut(s) 269
NcoI CCATGG 1 cut(s) 173
NdeII GATC 5 cut(s) 99, 154, 252, 285, 502
NlaIII CATG 6 cut(s) 55, 122, 177, 314, 367, 427
NlaIV GGNNCC 2 cut(s) 200, 461
NmuCI GTSAC 1 cut(s) 560
NspI RCATGY 2 cut(s) 122, 314
PagI TCATGA 1 cut(s) 423
PceI AGGCCT 1 cut(s) 599
PciI ACATGT 2 cut(s) 118, 310
PfeI GAWTC 1 cut(s) 181
PkrI GCNGC 2 cut(s) 431, 611
PpuMI RGGWCCY 1 cut(s) 199
PscI ACATGT 2 cut(s) 118, 310
Psp5II RGGWCCY 1 cut(s) 199
Psp6I CCWGG 1 cut(s) 267
PspGI CCWGG 1 cut(s) 267
PspN4I GGNNCC 2 cut(s) 200, 461
PspPI GGNCC 1 cut(s) 199
PspPPI RGGWCCY 1 cut(s) 199
RsaI GTAC 1 cut(s) 401
RsaNI GTAC 1 cut(s) 400
RseI CAYNNNNRTG 2 cut(s) 50, 74
SatI GCNGC 2 cut(s) 430, 610
Sau3AI GATC 5 cut(s) 99, 154, 252, 285, 502
Sau96I GGNCC 1 cut(s) 199
ScaI AGTACT 1 cut(s) 401
ScrFI CCNGG 1 cut(s) 269
SfaNI GCATC 1 cut(s) 20
SinI GGWCC 1 cut(s) 199
SmiMI CAYNNNNRTG 2 cut(s) 50, 74
SmlI CTYRAG 2 cut(s) 163, 206
SmoI CTYRAG 2 cut(s) 163, 206
SpeI ACTAGT 1 cut(s) 407
Sse9I AATT 5 cut(s) 342, 358, 439, 477, 581
SseBI AGGCCT 1 cut(s) 599
SspI AATATT 1 cut(s) 386
SspMI CTAG 5 cut(s) 24, 195, 408, 447, 486
StuI AGGCCT 1 cut(s) 599
StyD4I CCNGG 1 cut(s) 267
StyI CCWWGG 2 cut(s) 173, 369
TaaI ACNGT 1 cut(s) 9
TaiI ACGT 1 cut(s) 456
TaqI TCGA 3 cut(s) 115, 213, 288
TasI AATT 5 cut(s) 342, 358, 439, 477, 581
TatI WGTACW 1 cut(s) 399
TfiI GAWTC 1 cut(s) 181
TseFI GTSAC 1 cut(s) 560
TseI GCWGC 2 cut(s) 429, 609
Tsp45I GTSAC 1 cut(s) 560
TspDTI ATGAA 4 cut(s) 17, 58, 343, 412
VpaK11BI GGWCC 1 cut(s) 199
XceI RCATGY 2 cut(s) 122, 314
XspI CTAG 5 cut(s) 24, 195, 408, 447, 486
ZrmI AGTACT 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.