Rh3BG204300

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0002694
Physical Location & Seq
Forward (+)
18285527 .. 18288019
2493 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG204300.1

Sequence Viewer

Length: 1656 bp
ATGGCGTGTACTGATGGTGAAATCACAGTCGTAGAGCCAAATGGGGAGGAGGCAGTGGTGATTGATATTGACCCTGTAGCTGCTATGCGAAGGCATCTAGCGGTAGCGGAAAACGTAGTCACAACTCCTAGTGGGGGCTACCCTGGCTCTAGAGGAAGAGTTCCTCAAAGCCTCATCAACATCAGCCCAACAGCGATCGCTCCTGAAATGGTCTCCATCGGACCTTTTTACCACGGAAAGGAGATACTGGTGGATTTTGAAGACTCCAAATTGTGGTTTCTTAATCGACTCCTTTCTCGAACAAAGAGACATGGTGGGCCTGATCGTCTAAAAGATTTGTTTGAAGCCGTGCAAAAATTGGAGAATTTAGCGAAAGGATTCTATGATGAGGAAGTCCCCATGAGGAGCCCGGAGTTTATCAAAATGATGGTGCTTGATGGATGCTTTATCATTGAGCTGTTTCGACATCTTGGTAGAAATGAAGATGTCTTAGGTTTCAATAATCCTATCTCTGCCAAGCCATGGTTGATCCCAATTATCACTAGGGACCTCCTCAAGCTTGAAAACCAACTCCCTTTCTTCGTCCTTGAAAGATTGTTCAGAATTACTGCCCCTGCTGAAGATCATGGGGATCCTCTAGCTTTACTTGCCTTGAAGTTCTTTAATCTTTCCTTGCCTAGGCTTAATGATGATGTCCTTAAAAGTCTAATCCGTAACCCTCTACTAAGTCGTGAAATCGACAACCACTTGCTTGGCTTGTTTCACTACAGCGTCTGTTCCACTCGATCTACAAATGAAAAACATCAGCTGTCACCGTTCCGGCTTATCCCAGCACCTTTCCTGGCCTTGCCACAGCTCCCTCCGCTCCTAGAACAAAGCGAAGACCACCGAGTCAGTCCACTAAGGTACTGGAGTGTTTCAATCCCCATCTTTTGTTTAAGGTGGAGCGTTTCTACTATTTTTTATTGTTTGGGGTGGAGCATTGCAATTTTAAGACTCCTTTATAATGTCCTTTTCTATTGTTTGCGGTGGAGCGTTTCAATCCTTACAAGATTTCTTTCCCTAACTCTATCCAACTCTGAAGAGCATCGAGTCCTGTCAATCCAGTCTACCACACAACTGAGACCCTCTGGGGTCAAGTTTAAGCCACACAAAGCAGACAACTTCTTAGAGATCAATTTCACAAATAGGGTTCTACAAATCCCACCTATTACCATCAATGACCTCACCATTACCGTCTTCATCAACTGCCTAGCCTATGAACACTCGCACGATCGCGACCTCACCGATGGTAGCTTCACCCATTATATTGCCTTCTTGAGCTGTCTGATCAATTCACCTAGAGACGTCGCATTCCTTTGTGCTGAAGGGATTATTGCTAGTTACTCTCACAACGATCTTTATATAGCTGGGCTGTTCAACAGGTTGAGGGAGAAAGTTTATTTTCACAGAAATACTTGTGGTTACCTCGACGGGGAATTCAGAGCCGTGGAGGCTTATTACAGCAGCCATTGGGGTACCTTTATGCGAACTTATTTCAGCACTCCATGGTCCTTCATTTCAGTTGTTTCAGCCATCATCCTTCTTGTCCTCACCGGAGGCCAAACTGTTCTGGCTGTTTTGAGCTACATCGACCAGAGAGGAAAGCGACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

551

Amino Acids

62.78

Weight (kDa)

6.79

Isoelectric Point (pI)

48.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 53 - 531 2.4e-95 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1005
AasI GACNNNNNNGTC 1 cut(s) 890
AatII GACGTC 1 cut(s) 1350
Acc65I GGTACC 1 cut(s) 1517
AccB1I GGYRCC 1 cut(s) 1517
AccB7I CCANNNNNTGG 2 cut(s) 273, 522
AccBSI CCGCTC 1 cut(s) 865
AccI GTMKAC 1 cut(s) 1109
AccII CGCG 1 cut(s) 1278
AciI CCGC 4 cut(s) 101, 107, 863, 1027
AclWI GGATC 3 cut(s) 523, 626, 639
AcsI RAATTY 2 cut(s) 364, 1478
AcuI CTGAAG 3 cut(s) 639, 1101, 1386
AcyI GRCGYC 1 cut(s) 1347
AfaI GTAC 3 cut(s) 10, 908, 1519
AfiI CCNNNNNNNGG 6 cut(s) 134, 238, 273, 522, 678, 1474
AgsI TTSAA 9 cut(s) 260, 344, 499, 563, 590, 655, 921, 1041, 1420
AjnI CCWGG 2 cut(s) 142, 840
Alw26I GTCTC 4 cut(s) 217, 301, 1117, 1338
AlwI GGATC 3 cut(s) 523, 626, 639
AlwNI CAGNNNCTG 1 cut(s) 774
AoxI GGCC 3 cut(s) 317, 843, 1600
ApeKI GCWGC 2 cut(s) 80, 1506
ApoI RAATTY 2 cut(s) 364, 1478
AsiSI GCGATCGC 1 cut(s) 198
Asp700I GAANNNNTTC 1 cut(s) 377
Asp718I GGTACC 1 cut(s) 1517
AspA2I CCTAGG 1 cut(s) 677
AspS9I GGNCC 4 cut(s) 221, 317, 547, 1551
AsuC2I CCSGG 1 cut(s) 410
AsuHPI GGTGA 8 cut(s) 29, 70, 804, 1219, 1276, 1291, 1329, 1585
AvaII GGWCC 3 cut(s) 221, 547, 1551
AvrII CCTAGG 1 cut(s) 677
BaeI ACNNNNGTAYC 2 cut(s) 898, 931
BamHI GGATCC 1 cut(s) 631
BanI GGYRCC 1 cut(s) 1517
BanII GRGCYC 1 cut(s) 410
BbsI GAAGAC 3 cut(s) 267, 888, 1231
BbvI GCAGC 2 cut(s) 67, 1518
BccI CCATC 8 cut(s) 8, 224, 421, 431, 935, 1223, 1283, 1583
BceAI ACGGC 2 cut(s) 332, 1472
BciT130I CCWGG 2 cut(s) 144, 842
BclI TGATCA 1 cut(s) 1329
BcnI CCSGG 1 cut(s) 410
BcoDI GTCTC 4 cut(s) 217, 301, 1117, 1338
BfmI CTRYAG 2 cut(s) 75, 766
BglI GCCNNNNNGGC 1 cut(s) 1493
BisI GCNGC 2 cut(s) 81, 1507
BlnI CCTAGG 1 cut(s) 677
BlsI GCNGC 2 cut(s) 82, 1508
Bme1390I CCNGG 3 cut(s) 144, 410, 842
Bme18I GGWCC 3 cut(s) 221, 547, 1551
BmgT120I GGNCC 4 cut(s) 221, 317, 547, 1551
BmiI GGNNCC 4 cut(s) 407, 548, 633, 1519
BmrFI CCNGG 3 cut(s) 144, 410, 842
BmsI GCATC 3 cut(s) 103, 431, 1096
BpiI GAAGAC 3 cut(s) 267, 888, 1231
BpmI CTGGAG 1 cut(s) 931
BpuEI CTTGAG 2 cut(s) 539, 1339
BpuMI CCSGG 1 cut(s) 410
BsaHI GRCGYC 1 cut(s) 1347
BsaI GGTCTC 2 cut(s) 217, 1117
BsaJI CCNNGG 6 cut(s) 142, 232, 521, 677, 1488, 1547
BsaWI WCCGGW 1 cut(s) 1595
Bsc4I CCNNNNNNNGG 6 cut(s) 134, 238, 273, 522, 678, 1474
Bse1I ACTGG 3 cut(s) 252, 914, 1105
Bse3DI GCAATG 1 cut(s) 981
BseBI CCWGG 2 cut(s) 144, 842
BseDI CCNNGG 6 cut(s) 142, 232, 521, 677, 1488, 1547
BseGI GGATG 2 cut(s) 446, 1578
BseLI CCNNNNNNNGG 6 cut(s) 134, 238, 273, 522, 678, 1474
BseMI GCAATG 1 cut(s) 981
BseMII CTCAG 1 cut(s) 1112
BseNI ACTGG 3 cut(s) 252, 914, 1105
BseRI GAGGAG 3 cut(s) 62, 418, 542
BseXI GCAGC 2 cut(s) 67, 1518
BseYI CCCAGC 2 cut(s) 829, 1409
Bsh1236I CGCG 1 cut(s) 1278
Bsh1285I CGRYCG 2 cut(s) 198, 1276
BshFI GGCC 3 cut(s) 319, 845, 1602
BshNI GGYRCC 1 cut(s) 1517
BsiEI CGRYCG 2 cut(s) 198, 1276
BsiSI CCGG 3 cut(s) 410, 820, 1596
BslFI GGGAC 2 cut(s) 380, 560
BslI CCNNNNNNNGG 6 cut(s) 134, 238, 273, 522, 678, 1474
BsmAI GTCTC 4 cut(s) 217, 301, 1117, 1338
BsmBI CGTCTC 1 cut(s) 1338
BsmFI GGGAC 2 cut(s) 380, 560
BsmI GAATGC 1 cut(s) 1352
BsnI GGCC 3 cut(s) 319, 845, 1602
Bso31I GGTCTC 2 cut(s) 217, 1117
Bsp1286I GDGCHC 1 cut(s) 410
Bsp19I CCATGG 2 cut(s) 521, 1547
Bsp68I TCGCGA 1 cut(s) 1278
BspACI CCGC 4 cut(s) 101, 107, 863, 1027
BspANI GGCC 3 cut(s) 319, 845, 1602
BspCNI CTCAG 1 cut(s) 1113
BspFNI CGCG 1 cut(s) 1278
BspLI GGNNCC 4 cut(s) 407, 548, 633, 1519
BspPI GGATC 3 cut(s) 523, 626, 639
BspQI GCTCTTC 1 cut(s) 1077
BspT107I GGYRCC 1 cut(s) 1517
BspTNI GGTCTC 2 cut(s) 217, 1117
BsrBI CCGCTC 1 cut(s) 865
BsrDI GCAATG 1 cut(s) 981
BsrI ACTGG 3 cut(s) 252, 914, 1105
BssECI CCNNGG 6 cut(s) 142, 232, 521, 677, 1488, 1547
BssNI GRCGYC 1 cut(s) 1347
BssT1I CCWWGG 3 cut(s) 521, 677, 1547
Bst2UI CCWGG 2 cut(s) 144, 842
Bst4CI ACNGT 5 cut(s) 28, 816, 1237, 1609, 1653
Bst6I CTCTTC 2 cut(s) 151, 1077
BstACI GRCGYC 1 cut(s) 1347
BstDEI CTNAG 5 cut(s) 490, 725, 902, 1121, 1168
BstDSI CCRYGG 4 cut(s) 232, 521, 1488, 1547
BstEII GGTNACC 1 cut(s) 1463
BstENI CCTNNNNNAGG 1 cut(s) 676
BstF5I GGATG 2 cut(s) 446, 1578
BstFNI CGCG 1 cut(s) 1278
BstMAI GTCTC 4 cut(s) 217, 301, 1117, 1338
BstMCI CGRYCG 2 cut(s) 198, 1276
BstMWI GCNNNNNNNGC 4 cut(s) 144, 647, 862, 1493
BstNI CCWGG 2 cut(s) 144, 842
BstPI GGTNACC 1 cut(s) 1463
BstSCI CCNGG 3 cut(s) 142, 408, 840
BstSFI CTRYAG 2 cut(s) 75, 766
BstUI CGCG 1 cut(s) 1278
BstV1I GCAGC 2 cut(s) 67, 1518
BstV2I GAAGAC 3 cut(s) 267, 888, 1231
BstX2I RGATCY 1 cut(s) 631
BstXI CCANNNNNNTGG 1 cut(s) 752
BstYI RGATCY 1 cut(s) 631
BsuRI GGCC 3 cut(s) 319, 845, 1602
BtgI CCRYGG 4 cut(s) 232, 521, 1488, 1547
BtsCI GGATG 2 cut(s) 446, 1578
BtsI GCAGTG 1 cut(s) 60
BtsIMutI CAGTG 1 cut(s) 60
BtuMI TCGCGA 1 cut(s) 1278
CaiI CAGNNNCTG 1 cut(s) 774
Cfr13I GGNCC 4 cut(s) 221, 317, 547, 1551
CseI GACGC 1 cut(s) 760
Csp6I GTAC 3 cut(s) 9, 907, 1518
CviAII CATG 5 cut(s) 311, 400, 522, 626, 1548
CviQI GTAC 3 cut(s) 9, 907, 1518
DdeI CTNAG 5 cut(s) 490, 725, 902, 1121, 1168
DrdI GACNNNNNNGTC 1 cut(s) 890
DseDI GACNNNNNNGTC 1 cut(s) 890
Eam1104I CTCTTC 2 cut(s) 151, 1077
EarI CTCTTC 2 cut(s) 151, 1077
Eco130I CCWWGG 3 cut(s) 521, 677, 1547
Eco24I GRGCYC 1 cut(s) 410
Eco31I GGTCTC 2 cut(s) 217, 1117
Eco47I GGWCC 3 cut(s) 221, 547, 1551
Eco57I CTGAAG 3 cut(s) 639, 1101, 1386
Eco91I GGTNACC 1 cut(s) 1463
EcoNI CCTNNNNNAGG 1 cut(s) 676
EcoO109I RGGNCCY 1 cut(s) 547
EcoO65I GGTNACC 1 cut(s) 1463
EcoRI GAATTC 1 cut(s) 1478
EcoRII CCWGG 2 cut(s) 142, 840
EcoT14I CCWWGG 3 cut(s) 521, 677, 1547
EcoT38I GRGCYC 1 cut(s) 410
ErhI CCWWGG 3 cut(s) 521, 677, 1547
Esp3I CGTCTC 1 cut(s) 1338
FaeI CATG 5 cut(s) 314, 403, 525, 629, 1551
FaqI GGGAC 2 cut(s) 380, 560
FatI CATG 5 cut(s) 310, 399, 521, 625, 1547
FbaI TGATCA 1 cut(s) 1329
FblI GTMKAC 1 cut(s) 1109
Fnu4HI GCNGC 2 cut(s) 81, 1507
FokI GGATG 2 cut(s) 453, 1565
FriOI GRGCYC 1 cut(s) 410
Fsp4HI GCNGC 2 cut(s) 81, 1507
GluI GCNGC 2 cut(s) 81, 1507
GsaI CCCAGC 2 cut(s) 833, 1413
GsuI CTGGAG 1 cut(s) 931
HaeIII GGCC 3 cut(s) 319, 845, 1602
HapII CCGG 3 cut(s) 410, 820, 1596
HgaI GACGC 1 cut(s) 760
Hin1I GRCGYC 1 cut(s) 1347
Hin1II CATG 5 cut(s) 314, 403, 525, 629, 1551
HindIII AAGCTT 1 cut(s) 557
HinfI GANTC 6 cut(s) 263, 288, 378, 891, 996, 1092
HpaII CCGG 3 cut(s) 410, 820, 1596
HphI GGTGA 8 cut(s) 29, 70, 804, 1219, 1276, 1291, 1329, 1585
Hpy166II GTNNAC 3 cut(s) 9, 899, 1110
Hpy188I TCNGA 5 cut(s) 221, 602, 1081, 1329, 1484
Hpy188III TCNNGA 6 cut(s) 150, 203, 297, 731, 1277, 1318
Hpy8I GTNNAC 3 cut(s) 9, 899, 1110
Hpy99I CGWCG 2 cut(s) 1352, 1475
HpyAV CCTTC 5 cut(s) 84, 1324, 1361, 1564, 1592
HpyCH4III ACNGT 5 cut(s) 28, 816, 1237, 1609, 1653
HpyCH4IV ACGT 2 cut(s) 114, 1347
HpyCH4V TGCA 2 cut(s) 352, 986
HpyF10VI GCNNNNNNNGC 4 cut(s) 144, 647, 862, 1493
HpyF3I CTNAG 5 cut(s) 490, 725, 902, 1121, 1168
HpySE526I ACGT 2 cut(s) 114, 1347
Hsp92I GRCGYC 1 cut(s) 1347
Hsp92II CATG 5 cut(s) 314, 403, 525, 629, 1551
KpnI GGTACC 1 cut(s) 1521
Ksp22I TGATCA 1 cut(s) 1329
LguI GCTCTTC 1 cut(s) 1077
LmnI GCTCC 7 cut(s) 205, 405, 861, 870, 945, 978, 1032
Lsp1109I GCAGC 2 cut(s) 67, 1518
LweI GCATC 3 cut(s) 103, 431, 1096
MaeII ACGT 2 cut(s) 114, 1347
MaeIII GTNAC 5 cut(s) 118, 713, 810, 1382, 1463
MbiI CCGCTC 1 cut(s) 865
MboII GAAGA 8 cut(s) 168, 272, 494, 571, 632, 893, 1094, 1231
MflI RGATCY 1 cut(s) 631
MhlI GDGCHC 1 cut(s) 410
MluCI AATT 9 cut(s) 269, 356, 364, 534, 603, 987, 1177, 1333, 1478
MlyI GAGTC 5 cut(s) 257, 282, 900, 990, 1101
MmeI TCCRAC 1 cut(s) 1098
MroXI GAANNNNTTC 1 cut(s) 377
MseI TTAA 7 cut(s) 282, 663, 684, 699, 938, 992, 1143
MspA1I CMGCKG 1 cut(s) 808
MspI CCGG 3 cut(s) 410, 820, 1596
MspR9I CCNGG 3 cut(s) 144, 410, 842
Mva1269I GAATGC 1 cut(s) 1352
MvaI CCWGG 2 cut(s) 144, 842
MvnI CGCG 1 cut(s) 1278
MwoI GCNNNNNNNGC 4 cut(s) 144, 647, 862, 1493
NciI CCSGG 1 cut(s) 410
NcoI CCATGG 2 cut(s) 521, 1547
NlaIII CATG 5 cut(s) 314, 403, 525, 629, 1551
NlaIV GGNNCC 4 cut(s) 407, 548, 633, 1519
NmuCI GTSAC 2 cut(s) 118, 810
NruI TCGCGA 1 cut(s) 1278
PciSI GCTCTTC 1 cut(s) 1077
PctI GAATGC 1 cut(s) 1352
PdmI GAANNNNTTC 1 cut(s) 377
PfeI GAWTC 1 cut(s) 378
PflMI CCANNNNNTGG 2 cut(s) 273, 522
PkrI GCNGC 2 cut(s) 82, 1508
Ple19I CGATCG 2 cut(s) 198, 1276
PleI GAGTC 5 cut(s) 257, 282, 899, 990, 1100
PpsI GAGTC 5 cut(s) 257, 282, 899, 990, 1100
PpuMI RGGWCCY 1 cut(s) 547
PsiI TTATAA 1 cut(s) 1005
Psp5II RGGWCCY 1 cut(s) 547
Psp6I CCWGG 2 cut(s) 142, 840
PspEI GGTNACC 1 cut(s) 1463
PspFI CCCAGC 2 cut(s) 829, 1409
PspGI CCWGG 2 cut(s) 142, 840
PspN4I GGNNCC 4 cut(s) 407, 548, 633, 1519
PspPI GGNCC 4 cut(s) 221, 317, 547, 1551
PspPPI RGGWCCY 1 cut(s) 547
PstNI CAGNNNCTG 1 cut(s) 774
PsuI RGATCY 1 cut(s) 631
PvuI CGATCG 2 cut(s) 198, 1276
PvuII CAGCTG 1 cut(s) 808
RgaI GCGATCGC 1 cut(s) 198
RruI TCGCGA 1 cut(s) 1278
RsaI GTAC 3 cut(s) 10, 908, 1519
RsaNI GTAC 3 cut(s) 9, 907, 1518
SapI GCTCTTC 1 cut(s) 1077
SaqAI TTAA 7 cut(s) 282, 663, 684, 699, 938, 992, 1143
SatI GCNGC 2 cut(s) 81, 1507
Sau96I GGNCC 4 cut(s) 221, 317, 547, 1551
SchI GAGTC 5 cut(s) 257, 282, 900, 990, 1101
ScrFI CCNGG 3 cut(s) 144, 410, 842
SduI GDGCHC 1 cut(s) 410
SfaAI GCGATCGC 1 cut(s) 198
SfaNI GCATC 3 cut(s) 103, 431, 1096
SfcI CTRYAG 2 cut(s) 75, 766
SgfI GCGATCGC 1 cut(s) 198
SinI GGWCC 3 cut(s) 221, 547, 1551
SmlI CTYRAG 2 cut(s) 554, 1318
SmoI CTYRAG 2 cut(s) 554, 1318
Sse9I AATT 9 cut(s) 269, 356, 364, 534, 603, 987, 1177, 1333, 1478
SsiI CCGC 4 cut(s) 101, 107, 863, 1027
StyD4I CCNGG 3 cut(s) 142, 408, 840
StyI CCWWGG 3 cut(s) 521, 677, 1547
TaaI ACNGT 5 cut(s) 28, 816, 1237, 1609, 1653
TaiI ACGT 2 cut(s) 117, 1350
TaqI TCGA 8 cut(s) 286, 298, 463, 738, 784, 1090, 1470, 1632
TasI AATT 9 cut(s) 269, 356, 364, 534, 603, 987, 1177, 1333, 1478
TatI WGTACW 1 cut(s) 8
TfiI GAWTC 1 cut(s) 378
Tru1I TTAA 7 cut(s) 282, 663, 684, 699, 938, 992, 1143
Tru9I TTAA 7 cut(s) 282, 663, 684, 699, 938, 992, 1143
TscAI CASTG 1 cut(s) 60
TseFI GTSAC 2 cut(s) 118, 810
TseI GCWGC 2 cut(s) 80, 1506
Tsp45I GTSAC 2 cut(s) 118, 810
TspDTI ATGAA 5 cut(s) 495, 810, 1231, 1275, 1546
TspGWI ACGGA 2 cut(s) 249, 701
TspRI CASTG 1 cut(s) 60
Van91I CCANNNNNTGG 2 cut(s) 273, 522
VpaK11BI GGWCC 3 cut(s) 221, 547, 1551
XagI CCTNNNNNAGG 1 cut(s) 676
XapI RAATTY 2 cut(s) 364, 1478
XbaI TCTAGA 1 cut(s) 149
XcmI CCANNNNNNNNNTGG 1 cut(s) 906
XmaJI CCTAGG 1 cut(s) 677
XmiI GTMKAC 1 cut(s) 1109
XmnI GAANNNNTTC 1 cut(s) 377
ZraI GACGTC 1 cut(s) 1348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.