pycom14g02370

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0002694
Physical Location & Seq
Reverse (-)
1845987 .. 1847357
1371 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g02370.1

Sequence Viewer

Length: 1371 bp
ATGGCTTGCAGTGACGGCATCAAAGCAGTAGCAGAGCAAAATTGTACTGCTGAAGAAGTGGTGATTGATATCGACGAATCTGTAGCTGCTATGCGAAGTCGTCTTGAGCAAAACAGACGAGCAATTCCAACAACAAAAAATAGGAGATCTGTGTGCATATACAAAGTTCCTCCCGGCCTCACCGGAGTCAGCCCAAAATCCATTCAGCCTGAAATCGTCTCCATCGGGCCATACCACCGCGGCAAAAAGGAATTATCGGAATTCGAGAGCTACAAATGGTGGTTTCTTGACGGCCTCCTTTGGCGAACAAAGCGCGACCTAAAAGACTACATAGGAGCCATGAGCAGGTTGGAGGAAAGCACGAGAAGTTGCTATTCCGACCACCAGGAGTTGAGCGTTAGTGAATTTGTTCAAATGATGGTTCTTGATGGGTGCTTTATCATTGAGCTTTTTCGATATGTTTGTGAAAGTAGTGGCGATGATCACGTTGTTGATGAAAATGATCGGGATGGCAACCCCATCTTAGCCATGCCATGGTTGATTCCAATTCTCATGAGGGACCTCCTCAAGCTTGAAAATCAACTCCCTTTCTTCGTCCTCGAAAGATTATACGACATCTCATCGGATCATCGTAAAGGTGGGGATCCACTAAGCCTTCTTGCGGTTAAGTTCTTCAGTAACTCATTGCCTAGGCCTCCTGAAGTCCTTAAAAACTCAAGAATGATGGAGGCCGAGCACTTGCTTGACTTGTTTCACTTAACCTTCCTTCCCCTCCAAGGCACTTCAAATGATCAGTACTCTTCTCAAAAGTTATGTCATCCATCCTTGAAGTCAATCCAGTGTACCACAGAACTACGATCCTCAGGGATCAAGTTCAAGGCACGCAAAGCAGCGGACAGCTTCTTGGAGATCAATTTCCGAAATTGGGTTCTAGATATCCCACCTTTAACCATCAATGACTTCACCACTACTTTCATCATCAATTGCTTGGCCTTAGAACAATGTCGCCAATACACAGGTACGTGGTTCAGCACTTATATTACTTTCATGAGCTGTCTCCTGACTTCTACTAGAGATGTCAAGTTACTATGTTCCGATGGGATCATCAGTACCGGCTTCTCACTGAACGACCAAAGTGTAGCTGAGCTGTTCAGAAAGTTGGGGGAAAATGTTGGCTTAGAAATAAGAGACTGCTACCTTTCCAAGCAATTCAGAGATGTGGAGTCCTACTACAGCAGCCACTGGGCTACTCTCATGCGGACCTATTTTAGCAGGCCATGGTCCTTCATTTCAGTTTTTTACGCCTTCATCCTCCTTGTCCTCGTAGGTGCCCAAACTGCTATGGCCATCATGAGCTACTACAACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

457

Amino Acids

52.31

Weight (kDa)

5.85

Isoelectric Point (pI)

45.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 53 - 441 4.9e-109 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 336
AccB1I GGYRCC 1 cut(s) 1328
AccB7I CCANNNNNTGG 1 cut(s) 534
AccII CGCG 2 cut(s) 240, 315
AciI CCGC 5 cut(s) 238, 240, 662, 893, 1258
AclWI GGATC 6 cut(s) 633, 638, 651, 852, 875, 1109
AcoI YGGCCR 1 cut(s) 1344
AcsI RAATTY 2 cut(s) 260, 404
AcuI CTGAAG 3 cut(s) 72, 658, 720
AfaI GTAC 5 cut(s) 46, 797, 844, 1021, 1111
AfiI CCNNNNNNNGG 5 cut(s) 345, 534, 661, 776, 925
AgsI TTSAA 5 cut(s) 413, 575, 786, 829, 877
AjnI CCWGG 1 cut(s) 384
AluBI AGCT 9 cut(s) 86, 270, 448, 571, 900, 1053, 1142, 1147, 1356
AluI AGCT 9 cut(s) 86, 270, 448, 571, 900, 1053, 1142, 1147, 1356
Alw21I GWGCWC 1 cut(s) 738
Alw26I GTCTC 3 cut(s) 223, 1061, 1182
AlwI GGATC 6 cut(s) 633, 638, 651, 852, 875, 1109
AlwNI CAGNNNCTG 1 cut(s) 1242
AoxI GGCC 8 cut(s) 175, 227, 292, 692, 729, 990, 1274, 1344
ApeKI GCWGC 3 cut(s) 86, 890, 1236
ApoI RAATTY 2 cut(s) 260, 404
ArsI GACNNNNNNTTYG 2 cut(s) 799, 831
Asp700I GAANNNNTTC 1 cut(s) 408
AspA2I CCTAGG 1 cut(s) 689
AspLEI GCGC 1 cut(s) 315
AspS9I GGNCC 4 cut(s) 227, 559, 1260, 1281
AsuC2I CCSGG 1 cut(s) 174
AsuHPI GGTGA 3 cut(s) 73, 172, 955
AvaII GGWCC 3 cut(s) 559, 1260, 1281
AvrII CCTAGG 1 cut(s) 689
AxyI CCTNAGG 1 cut(s) 862
BaeGI GKGCMC 1 cut(s) 1333
BalI TGGCCA 1 cut(s) 1346
BamHI GGATCC 1 cut(s) 643
BanI GGYRCC 1 cut(s) 1328
BauI CACGAG 1 cut(s) 361
Bbv12I GWGCWC 1 cut(s) 738
BbvI GCAGC 3 cut(s) 73, 902, 1248
BceAI ACGGC 2 cut(s) 31, 307
BciT130I CCWGG 1 cut(s) 386
BclI TGATCA 2 cut(s) 481, 790
BcnI CCSGG 1 cut(s) 174
BcoDI GTCTC 3 cut(s) 223, 1061, 1182
BfaI CTAG 3 cut(s) 690, 932, 1071
BfmI CTRYAG 2 cut(s) 81, 1231
BfuAI ACCTGC 1 cut(s) 336
BglII AGATCT 1 cut(s) 146
BisI GCNGC 4 cut(s) 87, 241, 891, 1237
BlnI CCTAGG 1 cut(s) 689
BlpI GCTNAGC 1 cut(s) 1143
BlsI GCNGC 4 cut(s) 88, 242, 892, 1238
BmcAI AGTACT 1 cut(s) 797
Bme1390I CCNGG 2 cut(s) 174, 386
Bme18I GGWCC 3 cut(s) 559, 1260, 1281
BmgT120I GGNCC 4 cut(s) 227, 559, 1260, 1281
BmiI GGNNCC 4 cut(s) 337, 560, 645, 1330
BmrFI CCNGG 2 cut(s) 174, 386
BmrI ACTGGG 1 cut(s) 1252
BmsI GCATC 1 cut(s) 27
BmuI ACTGGG 1 cut(s) 1252
Bpu1102I GCTNAGC 1 cut(s) 1143
BpuEI CTTGAG 3 cut(s) 125, 551, 700
BpuMI CCSGG 1 cut(s) 174
BsaAI YACGTR 1 cut(s) 1023
BsaBI GATNNNNATC 1 cut(s) 68
BsaJI CCNNGG 5 cut(s) 238, 533, 689, 775, 1277
BsaWI WCCGGW 1 cut(s) 182
Bsc4I CCNNNNNNNGG 5 cut(s) 345, 534, 661, 776, 925
Bse118I RCCGGY 1 cut(s) 1112
Bse1I ACTGG 2 cut(s) 838, 1247
Bse21I CCTNAGG 1 cut(s) 862
Bse3DI GCAATG 1 cut(s) 683
Bse8I GATNNNNATC 1 cut(s) 68
BseBI CCWGG 1 cut(s) 386
BseDI CCNNGG 5 cut(s) 238, 533, 689, 775, 1277
BseGI GGATG 4 cut(s) 514, 817, 821, 1308
BseJI GATNNNNATC 1 cut(s) 68
BseLI CCNNNNNNNGG 5 cut(s) 345, 534, 661, 776, 925
BseMI GCAATG 1 cut(s) 683
BseMII CTCAG 2 cut(s) 876, 1134
BseNI ACTGG 2 cut(s) 838, 1247
BseRI GAGGAG 1 cut(s) 554
BseSI GKGCMC 1 cut(s) 1333
BseXI GCAGC 3 cut(s) 73, 902, 1248
Bsh1236I CGCG 2 cut(s) 240, 315
BshFI GGCC 8 cut(s) 177, 229, 294, 694, 731, 992, 1276, 1346
BshNI GGYRCC 1 cut(s) 1328
BsiHKAI GWGCWC 1 cut(s) 738
BsiSI CCGG 3 cut(s) 174, 183, 1113
BslFI GGGAC 1 cut(s) 572
BslI CCNNNNNNNGG 5 cut(s) 345, 534, 661, 776, 925
BsmAI GTCTC 3 cut(s) 223, 1061, 1182
BsmBI CGTCTC 1 cut(s) 223
BsmFI GGGAC 1 cut(s) 572
BsnI GGCC 8 cut(s) 177, 229, 294, 694, 731, 992, 1276, 1346
Bsp1286I GDGCHC 2 cut(s) 738, 1333
Bsp1720I GCTNAGC 1 cut(s) 1143
Bsp19I CCATGG 2 cut(s) 533, 1277
BspACI CCGC 5 cut(s) 238, 240, 662, 893, 1258
BspANI GGCC 8 cut(s) 177, 229, 294, 694, 731, 992, 1276, 1346
BspCNI CTCAG 2 cut(s) 875, 1135
BspFNI CGCG 2 cut(s) 240, 315
BspHI TCATGA 3 cut(s) 552, 1047, 1350
BspLI GGNNCC 4 cut(s) 337, 560, 645, 1330
BspMI ACCTGC 1 cut(s) 336
BspPI GGATC 6 cut(s) 633, 638, 651, 852, 875, 1109
BspT107I GGYRCC 1 cut(s) 1328
BsrDI GCAATG 1 cut(s) 683
BsrFI RCCGGY 1 cut(s) 1112
BsrI ACTGG 2 cut(s) 838, 1247
BssAI RCCGGY 1 cut(s) 1112
BssECI CCNNGG 5 cut(s) 238, 533, 689, 775, 1277
BssSI CACGAG 1 cut(s) 361
BssT1I CCWWGG 4 cut(s) 533, 689, 775, 1277
Bst2BI CACGAG 1 cut(s) 361
Bst2UI CCWGG 1 cut(s) 386
Bst6I CTCTTC 1 cut(s) 805
BstBAI YACGTR 1 cut(s) 1023
BstC8I GCNNGC 3 cut(s) 7, 883, 1274
BstDEI CTNAG 6 cut(s) 523, 650, 862, 994, 1143, 1177
BstDSI CCRYGG 3 cut(s) 238, 533, 1277
BstF5I GGATG 4 cut(s) 514, 817, 821, 1308
BstFNI CGCG 2 cut(s) 240, 315
BstHHI GCGC 1 cut(s) 315
BstMAI GTCTC 3 cut(s) 223, 1061, 1182
BstMWI GCNNNNNNNGC 4 cut(s) 15, 310, 887, 1337
BstNI CCWGG 1 cut(s) 386
BstSCI CCNGG 2 cut(s) 172, 384
BstSFI CTRYAG 2 cut(s) 81, 1231
BstSLI GKGCMC 1 cut(s) 1333
BstUI CGCG 2 cut(s) 240, 315
BstV1I GCAGC 3 cut(s) 73, 902, 1248
BstX2I RGATCY 2 cut(s) 146, 643
BstYI RGATCY 2 cut(s) 146, 643
Bsu36I CCTNAGG 1 cut(s) 862
BsuRI GGCC 8 cut(s) 177, 229, 294, 694, 731, 992, 1276, 1346
BtgI CCRYGG 3 cut(s) 238, 533, 1277
BtgZI GCGATG 1 cut(s) 492
BtsCI GGATG 4 cut(s) 514, 817, 821, 1308
BtsI GCAGTG 1 cut(s) 16
BtsIMutI CAGTG 4 cut(s) 16, 845, 1121, 1240
BveI ACCTGC 1 cut(s) 336
Cac8I GCNNGC 3 cut(s) 7, 883, 1274
CaiI CAGNNNCTG 1 cut(s) 1242
CciI TCATGA 3 cut(s) 552, 1047, 1350
CfoI GCGC 1 cut(s) 315
Cfr10I RCCGGY 1 cut(s) 1112
Cfr13I GGNCC 4 cut(s) 227, 559, 1260, 1281
Cfr42I CCGCGG 1 cut(s) 241
Csp6I GTAC 5 cut(s) 45, 796, 843, 1020, 1110
CviAII CATG 8 cut(s) 340, 529, 534, 553, 1048, 1255, 1278, 1351
CviQI GTAC 5 cut(s) 45, 796, 843, 1020, 1110
DdeI CTNAG 6 cut(s) 523, 650, 862, 994, 1143, 1177
EaeI YGGCCR 1 cut(s) 1344
Eam1104I CTCTTC 1 cut(s) 805
EarI CTCTTC 1 cut(s) 805
Eco130I CCWWGG 4 cut(s) 533, 689, 775, 1277
Eco147I AGGCCT 1 cut(s) 694
Eco32I GATATC 2 cut(s) 70, 937
Eco47I GGWCC 3 cut(s) 559, 1260, 1281
Eco57I CTGAAG 3 cut(s) 72, 658, 720
Eco81I CCTNAGG 1 cut(s) 862
EcoO109I RGGNCCY 1 cut(s) 559
EcoRI GAATTC 1 cut(s) 260
EcoRII CCWGG 1 cut(s) 384
EcoRV GATATC 2 cut(s) 70, 937
EcoT14I CCWWGG 4 cut(s) 533, 689, 775, 1277
ErhI CCWWGG 4 cut(s) 533, 689, 775, 1277
Esp3I CGTCTC 1 cut(s) 223
FaeI CATG 8 cut(s) 343, 532, 537, 556, 1051, 1258, 1281, 1354
FaqI GGGAC 1 cut(s) 572
FatI CATG 8 cut(s) 339, 528, 533, 552, 1047, 1254, 1277, 1350
FbaI TGATCA 2 cut(s) 481, 790
Fnu4HI GCNGC 4 cut(s) 87, 241, 891, 1237
FokI GGATG 4 cut(s) 521, 804, 808, 1295
Fsp4HI GCNGC 4 cut(s) 87, 241, 891, 1237
FspBI CTAG 3 cut(s) 690, 932, 1071
GlaI GCGC 1 cut(s) 314
GluI GCNGC 4 cut(s) 87, 241, 891, 1237
HaeIII GGCC 8 cut(s) 177, 229, 294, 694, 731, 992, 1276, 1346
HapII CCGG 3 cut(s) 174, 183, 1113
HhaI GCGC 1 cut(s) 315
Hin1II CATG 8 cut(s) 343, 532, 537, 556, 1051, 1258, 1281, 1354
Hin6I GCGC 1 cut(s) 313
HinP1I GCGC 1 cut(s) 313
HindIII AAGCTT 1 cut(s) 569
HinfI GANTC 4 cut(s) 77, 186, 541, 1223
HpaII CCGG 3 cut(s) 174, 183, 1113
HphI GGTGA 3 cut(s) 73, 172, 955
Hpy166II GTNNAC 1 cut(s) 843
Hpy188I TCNGA 7 cut(s) 259, 379, 625, 920, 1096, 1154, 1214
Hpy8I GTNNAC 1 cut(s) 843
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 5 cut(s) 665, 772, 776, 1294, 1315
HpyCH4IV ACGT 2 cut(s) 486, 1022
HpyCH4V TGCA 2 cut(s) 9, 156
HpyF10VI GCNNNNNNNGC 4 cut(s) 15, 310, 887, 1337
HpyF3I CTNAG 6 cut(s) 523, 650, 862, 994, 1143, 1177
HpySE526I ACGT 2 cut(s) 486, 1022
Hsp92II CATG 8 cut(s) 343, 532, 537, 556, 1051, 1258, 1281, 1354
HspAI GCGC 1 cut(s) 313
Ksp22I TGATCA 2 cut(s) 481, 790
KspI CCGCGG 1 cut(s) 241
LmnI GCTCC 1 cut(s) 335
Lsp1109I GCAGC 3 cut(s) 73, 902, 1248
LweI GCATC 1 cut(s) 27
MaeI CTAG 3 cut(s) 690, 932, 1071
MaeII ACGT 2 cut(s) 486, 1022
MaeIII GTNAC 3 cut(s) 11, 677, 1083
MboII GAAGA 4 cut(s) 65, 583, 664, 792
MfeI CAATTG 1 cut(s) 982
MflI RGATCY 2 cut(s) 146, 643
MhlI GDGCHC 2 cut(s) 738, 1333
MlsI TGGCCA 1 cut(s) 1346
MluNI TGGCCA 1 cut(s) 1346
MlyI GAGTC 2 cut(s) 195, 1232
MmeI TCCRAC 3 cut(s) 152, 330, 402
Mox20I TGGCCA 1 cut(s) 1346
MroXI GAANNNNTTC 1 cut(s) 408
MscI TGGCCA 1 cut(s) 1346
MseI TTAA 5 cut(s) 666, 708, 758, 947, 1369
Msp20I TGGCCA 1 cut(s) 1346
MspA1I CMGCKG 2 cut(s) 240, 893
MspI CCGG 3 cut(s) 174, 183, 1113
MspR9I CCNGG 2 cut(s) 174, 386
MunI CAATTG 1 cut(s) 982
MvaI CCWGG 1 cut(s) 386
MvnI CGCG 2 cut(s) 240, 315
MwoI GCNNNNNNNGC 4 cut(s) 15, 310, 887, 1337
NciI CCSGG 1 cut(s) 174
NcoI CCATGG 2 cut(s) 533, 1277
NlaIII CATG 8 cut(s) 343, 532, 537, 556, 1051, 1258, 1281, 1354
NlaIV GGNNCC 4 cut(s) 337, 560, 645, 1330
NmeAIII GCCGAG 1 cut(s) 757
NmuCI GTSAC 1 cut(s) 11
PagI TCATGA 3 cut(s) 552, 1047, 1350
PceI AGGCCT 1 cut(s) 694
PdmI GAANNNNTTC 1 cut(s) 408
PfeI GAWTC 2 cut(s) 77, 541
PflMI CCANNNNNTGG 1 cut(s) 534
PkrI GCNGC 4 cut(s) 88, 242, 892, 1238
PleI GAGTC 2 cut(s) 194, 1231
PpsI GAGTC 2 cut(s) 194, 1231
Ppu21I YACGTR 1 cut(s) 1023
PpuMI RGGWCCY 1 cut(s) 559
Psp5II RGGWCCY 1 cut(s) 559
Psp6I CCWGG 1 cut(s) 384
PspGI CCWGG 1 cut(s) 384
PspN4I GGNNCC 4 cut(s) 337, 560, 645, 1330
PspPI GGNCC 4 cut(s) 227, 559, 1260, 1281
PspPPI RGGWCCY 1 cut(s) 559
PstNI CAGNNNCTG 1 cut(s) 1242
PsuI RGATCY 2 cut(s) 146, 643
RsaI GTAC 5 cut(s) 46, 797, 844, 1021, 1111
RsaNI GTAC 5 cut(s) 45, 796, 843, 1020, 1110
SacII CCGCGG 1 cut(s) 241
SaqAI TTAA 5 cut(s) 666, 708, 758, 947, 1369
SatI GCNGC 4 cut(s) 87, 241, 891, 1237
Sau96I GGNCC 4 cut(s) 227, 559, 1260, 1281
ScaI AGTACT 1 cut(s) 797
SchI GAGTC 2 cut(s) 195, 1232
ScrFI CCNGG 2 cut(s) 174, 386
SduI GDGCHC 2 cut(s) 738, 1333
SfaNI GCATC 1 cut(s) 27
SfcI CTRYAG 2 cut(s) 81, 1231
Sfr303I CCGCGG 1 cut(s) 241
SgrBI CCGCGG 1 cut(s) 241
SinI GGWCC 3 cut(s) 559, 1260, 1281
SmlI CTYRAG 3 cut(s) 104, 566, 715
SmoI CTYRAG 3 cut(s) 104, 566, 715
SseBI AGGCCT 1 cut(s) 694
SsiI CCGC 5 cut(s) 238, 240, 662, 893, 1258
SspMI CTAG 3 cut(s) 690, 932, 1071
StuI AGGCCT 1 cut(s) 694
StyD4I CCNGG 2 cut(s) 172, 384
StyI CCWWGG 4 cut(s) 533, 689, 775, 1277
TaiI ACGT 2 cut(s) 489, 1025
TaqI TCGA 4 cut(s) 72, 264, 454, 600
TatI WGTACW 2 cut(s) 44, 795
TauI GCSGC 1 cut(s) 243
TfiI GAWTC 2 cut(s) 77, 541
Tru1I TTAA 5 cut(s) 666, 708, 758, 947, 1369
Tru9I TTAA 5 cut(s) 666, 708, 758, 947, 1369
TscAI CASTG 4 cut(s) 16, 845, 1128, 1247
TseFI GTSAC 1 cut(s) 11
TseI GCWGC 3 cut(s) 86, 890, 1236
Tsp45I GTSAC 1 cut(s) 11
TspDTI ATGAA 5 cut(s) 510, 964, 1036, 1276, 1297
TspRI CASTG 4 cut(s) 16, 845, 1128, 1247
Van91I CCANNNNNTGG 1 cut(s) 534
VpaK11BI GGWCC 3 cut(s) 559, 1260, 1281
XapI RAATTY 2 cut(s) 260, 404
XbaI TCTAGA 1 cut(s) 931
XcmI CCANNNNNNNNNTGG 1 cut(s) 346
XmaJI CCTAGG 1 cut(s) 689
XmnI GAANNNNTTC 1 cut(s) 408
XspI CTAG 3 cut(s) 690, 932, 1071
ZrmI AGTACT 1 cut(s) 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.