Rh3AG179300

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0002694
Physical Location & Seq
Forward (+)
16787829 .. 16788632
804 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG179300.1

Sequence Viewer

Length: 804 bp
ATGGTCAACTTTAGTTCAGTTGTCTGCATATACAGAGTTCCTGAAAGCCTTGTGGGAATCAGTCCAAAAGCTATTAGGCCTGAAAAGGTCTCCATCGGACCTTATCACCACAGCAAGAGCAAGGAATCTGAAGAGACCAAAATGAAGTATTTTGAAGCTTTTCTCTCTAGAAAAAAGAGAGATGGTAGTGCTGGACCTGATCTAAGTACCTTGTTTAAAGCCGCGAGAAATATGGAGGCAGAAGCAAGAAGATGCTATGATGATCAGGCCATCGCTATGTCAAGCGCGGAGTTTGTTGCAATGATGGTACTTGATGGGTGCTTTATCATTGAGCTTTTCCTACAAGACCAAGAGGACAAACTCAAAGATGTTAAAGAGAAGGACCGCCTGGTTATGGACCTCCTCAAGCTTGAAAACCAACTGCCTTTCTTCGTCCTTGAAAGACTATTTAGCCTGTCCGGTGATTCATCTGCACCAGGTGATTCTCTAGCCTTGCTTGCCTTGAAGTTCTTCAATGTTTATGATCACAAAGTCCTGGATGATGTTAAACACTTGTTACACTTGTTTCACTCAAGCTTCCGTACCCCTTTATTATTAGAACGAGATAATACTCGATATCAGCTCTCTAATATTAGAAGCCGCTGCGTAGACTTACTTGGGGAAGCTGATGATGTAATGATTGCATATGTAACGACATGCCTATCAATCGACAGCCCAGCGACCGCAATGAGGTGCTTCCGTGCTTACTTGCAACCCCTTTTCTATTTGTTTGGGGGATCCTTGCATTCCTTTATCGTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

267

Amino Acids

30.39

Weight (kDa)

6.18

Isoelectric Point (pI)

41.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 10 - 219 4.9e-46 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 648
AccII CGCG 2 cut(s) 224, 287
AciI CCGC 5 cut(s) 222, 287, 385, 640, 723
AclWI GGATC 2 cut(s) 771, 784
AcuI CTGAAG 1 cut(s) 150
AdeI CACNNNGTG 1 cut(s) 479
AfaI GTAC 3 cut(s) 208, 309, 583
AfiI CCNNNNNNNGG 2 cut(s) 394, 729
AgsI TTSAA 5 cut(s) 155, 413, 440, 505, 514
AjnI CCWGG 3 cut(s) 387, 475, 534
AluBI AGCT 7 cut(s) 71, 158, 334, 409, 576, 622, 665
AluI AGCT 7 cut(s) 71, 158, 334, 409, 576, 622, 665
Alw26I GTCTC 2 cut(s) 94, 128
AlwI GGATC 2 cut(s) 771, 784
AoxI GGCC 2 cut(s) 77, 267
ApeKI GCWGC 1 cut(s) 642
Asp700I GAANNNNTTC 2 cut(s) 159, 509
AspLEI GCGC 1 cut(s) 287
AspS9I GGNCC 4 cut(s) 98, 194, 382, 397
AsuHPI GGTGA 3 cut(s) 98, 473, 491
AvaII GGWCC 4 cut(s) 98, 194, 382, 397
BamHI GGATCC 1 cut(s) 776
BbsI GAAGAC 1 cut(s) 790
BbvI GCAGC 1 cut(s) 629
BccI CCATC 5 cut(s) 101, 176, 278, 298, 308
BciT130I CCWGG 3 cut(s) 389, 477, 536
BclI TGATCA 2 cut(s) 262, 523
BcoDI GTCTC 2 cut(s) 94, 128
BfaI CTAG 2 cut(s) 168, 488
BisI GCNGC 3 cut(s) 222, 640, 643
BlsI GCNGC 3 cut(s) 223, 641, 644
Bme1390I CCNGG 3 cut(s) 389, 477, 536
Bme18I GGWCC 4 cut(s) 98, 194, 382, 397
BmgT120I GGNCC 4 cut(s) 98, 194, 382, 397
BmiI GGNNCC 1 cut(s) 778
BmrFI CCNGG 3 cut(s) 389, 477, 536
BmsI GCATC 1 cut(s) 242
BpiI GAAGAC 1 cut(s) 790
BpuEI CTTGAG 2 cut(s) 389, 556
BsaI GGTCTC 2 cut(s) 94, 128
BsaWI WCCGGW 1 cut(s) 458
Bsc4I CCNNNNNNNGG 2 cut(s) 394, 729
Bse3DI GCAATG 2 cut(s) 306, 732
BseBI CCWGG 3 cut(s) 389, 477, 536
BseGI GGATG 1 cut(s) 544
BseLI CCNNNNNNNGG 2 cut(s) 394, 729
BseMI GCAATG 2 cut(s) 306, 732
BseRI GAGGAG 1 cut(s) 392
BseXI GCAGC 1 cut(s) 629
BseYI CCCAGC 1 cut(s) 715
BsgI GTGCAG 1 cut(s) 456
Bsh1236I CGCG 2 cut(s) 224, 287
Bsh1285I CGRYCG 1 cut(s) 723
BshFI GGCC 2 cut(s) 79, 269
BsiEI CGRYCG 1 cut(s) 723
BsiSI CCGG 1 cut(s) 459
BslI CCNNNNNNNGG 2 cut(s) 394, 729
BsmAI GTCTC 2 cut(s) 94, 128
BsmI GAATGC 1 cut(s) 784
BsnI GGCC 2 cut(s) 79, 269
Bso31I GGTCTC 2 cut(s) 94, 128
Bsp143I GATC 4 cut(s) 199, 262, 523, 776
BspACI CCGC 5 cut(s) 222, 287, 385, 640, 723
BspANI GGCC 2 cut(s) 79, 269
BspFNI CGCG 2 cut(s) 224, 287
BspLI GGNNCC 1 cut(s) 778
BspPI GGATC 2 cut(s) 771, 784
BspTNI GGTCTC 2 cut(s) 94, 128
BsrDI GCAATG 2 cut(s) 306, 732
BssMI GATC 4 cut(s) 199, 262, 523, 776
Bst2UI CCWGG 3 cut(s) 389, 477, 536
Bst6I CTCTTC 1 cut(s) 126
BstC8I GCNNGC 1 cut(s) 498
BstDEI CTNAG 1 cut(s) 203
BstF5I GGATG 1 cut(s) 544
BstFNI CGCG 2 cut(s) 224, 287
BstHHI GCGC 1 cut(s) 287
BstKTI GATC 4 cut(s) 202, 265, 526, 779
BstMAI GTCTC 2 cut(s) 94, 128
BstMBI GATC 4 cut(s) 199, 262, 523, 776
BstMCI CGRYCG 1 cut(s) 723
BstMWI GCNNNNNNNGC 1 cut(s) 497
BstNI CCWGG 3 cut(s) 389, 477, 536
BstNSI RCATGY 1 cut(s) 699
BstSCI CCNGG 3 cut(s) 387, 475, 534
BstUI CGCG 2 cut(s) 224, 287
BstV1I GCAGC 1 cut(s) 629
BstV2I GAAGAC 1 cut(s) 790
BstX2I RGATCY 1 cut(s) 776
BstYI RGATCY 1 cut(s) 776
BsuRI GGCC 2 cut(s) 79, 269
BtgZI GCGATG 1 cut(s) 256
BtsCI GGATG 1 cut(s) 544
Cac8I GCNNGC 1 cut(s) 498
CfoI GCGC 1 cut(s) 287
Cfr13I GGNCC 4 cut(s) 98, 194, 382, 397
CsiI ACCWGGT 1 cut(s) 475
Csp6I GTAC 3 cut(s) 207, 308, 582
CviAII CATG 1 cut(s) 696
CviQI GTAC 3 cut(s) 207, 308, 582
DdeI CTNAG 1 cut(s) 203
DpnI GATC 4 cut(s) 201, 264, 525, 778
DpnII GATC 4 cut(s) 199, 262, 523, 776
DraI TTTAAA 1 cut(s) 217
DraIII CACNNNGTG 1 cut(s) 479
Eam1104I CTCTTC 1 cut(s) 126
EarI CTCTTC 1 cut(s) 126
Eco147I AGGCCT 1 cut(s) 79
Eco31I GGTCTC 2 cut(s) 94, 128
Eco32I GATATC 1 cut(s) 617
Eco47I GGWCC 4 cut(s) 98, 194, 382, 397
Eco57I CTGAAG 1 cut(s) 150
EcoRII CCWGG 3 cut(s) 387, 475, 534
EcoRV GATATC 1 cut(s) 617
FaeI CATG 1 cut(s) 699
FatI CATG 1 cut(s) 695
FauNDI CATATG 1 cut(s) 685
FbaI TGATCA 2 cut(s) 262, 523
FblI GTMKAC 1 cut(s) 648
Fnu4HI GCNGC 3 cut(s) 222, 640, 643
FokI GGATG 1 cut(s) 551
Fsp4HI GCNGC 3 cut(s) 222, 640, 643
FspBI CTAG 2 cut(s) 168, 488
GlaI GCGC 1 cut(s) 286
GluI GCNGC 3 cut(s) 222, 640, 643
GsaI CCCAGC 1 cut(s) 719
HaeIII GGCC 2 cut(s) 79, 269
HapII CCGG 1 cut(s) 459
HhaI GCGC 1 cut(s) 287
Hin1II CATG 1 cut(s) 699
Hin6I GCGC 1 cut(s) 285
HinP1I GCGC 1 cut(s) 285
HincII GTYRAC 1 cut(s) 7
HindII GTYRAC 1 cut(s) 7
HindIII AAGCTT 3 cut(s) 156, 407, 574
HinfI GANTC 4 cut(s) 57, 125, 464, 482
HpaII CCGG 1 cut(s) 459
HphI GGTGA 3 cut(s) 98, 473, 491
Hpy166II GTNNAC 2 cut(s) 7, 649
Hpy188I TCNGA 2 cut(s) 98, 130
Hpy188III TCNNGA 2 cut(s) 41, 168
Hpy8I GTNNAC 2 cut(s) 7, 649
HpyAV CCTTC 1 cut(s) 373
HpyCH4V TGCA 6 cut(s) 27, 299, 473, 683, 751, 784
HpyF10VI GCNNNNNNNGC 1 cut(s) 497
HpyF3I CTNAG 1 cut(s) 203
Hsp92II CATG 1 cut(s) 699
HspAI GCGC 1 cut(s) 285
Ksp22I TGATCA 2 cut(s) 262, 523
Kzo9I GATC 4 cut(s) 199, 262, 523, 776
Lsp1109I GCAGC 1 cut(s) 629
LweI GCATC 1 cut(s) 242
MabI ACCWGGT 1 cut(s) 475
MaeI CTAG 2 cut(s) 168, 488
MaeIII GTNAC 2 cut(s) 555, 688
MalI GATC 4 cut(s) 201, 264, 525, 778
MboI GATC 4 cut(s) 199, 262, 523, 776
MboII GAAGA 5 cut(s) 143, 261, 421, 502, 790
MflI RGATCY 1 cut(s) 776
MnlI CCTC 5 cut(s) 229, 346, 410, 413, 723
MroXI GAANNNNTTC 2 cut(s) 159, 509
MseI TTAA 3 cut(s) 216, 372, 546
MslI CAYNNNNRTG 1 cut(s) 275
MspA1I CMGCKG 1 cut(s) 642
MspI CCGG 1 cut(s) 459
MspR9I CCNGG 3 cut(s) 389, 477, 536
Mva1269I GAATGC 1 cut(s) 784
MvaI CCWGG 3 cut(s) 389, 477, 536
MvnI CGCG 2 cut(s) 224, 287
MwoI GCNNNNNNNGC 1 cut(s) 497
NdeI CATATG 1 cut(s) 685
NdeII GATC 4 cut(s) 199, 262, 523, 776
NlaIII CATG 1 cut(s) 699
NlaIV GGNNCC 1 cut(s) 778
NspI RCATGY 1 cut(s) 699
PceI AGGCCT 1 cut(s) 79
PctI GAATGC 1 cut(s) 784
PdmI GAANNNNTTC 2 cut(s) 159, 509
PfeI GAWTC 4 cut(s) 57, 125, 464, 482
PfoI TCCNGGA 1 cut(s) 534
PkrI GCNGC 3 cut(s) 223, 641, 644
Psp6I CCWGG 3 cut(s) 387, 475, 534
PspFI CCCAGC 1 cut(s) 715
PspGI CCWGG 3 cut(s) 387, 475, 534
PspN4I GGNNCC 1 cut(s) 778
PspPI GGNCC 4 cut(s) 98, 194, 382, 397
PsuI RGATCY 1 cut(s) 776
RsaI GTAC 3 cut(s) 208, 309, 583
RsaNI GTAC 3 cut(s) 207, 308, 582
RseI CAYNNNNRTG 1 cut(s) 275
SaqAI TTAA 3 cut(s) 216, 372, 546
SatI GCNGC 3 cut(s) 222, 640, 643
Sau3AI GATC 4 cut(s) 199, 262, 523, 776
Sau96I GGNCC 4 cut(s) 98, 194, 382, 397
ScrFI CCNGG 3 cut(s) 389, 477, 536
SexAI ACCWGGT 1 cut(s) 475
SfaNI GCATC 1 cut(s) 242
SinI GGWCC 4 cut(s) 98, 194, 382, 397
SmiMI CAYNNNNRTG 1 cut(s) 275
SmlI CTYRAG 2 cut(s) 404, 571
SmoI CTYRAG 2 cut(s) 404, 571
SseBI AGGCCT 1 cut(s) 79
SsiI CCGC 5 cut(s) 222, 287, 385, 640, 723
SspI AATATT 1 cut(s) 631
SspMI CTAG 2 cut(s) 168, 488
StuI AGGCCT 1 cut(s) 79
StyD4I CCNGG 3 cut(s) 387, 475, 534
TaqI TCGA 2 cut(s) 613, 708
TauI GCSGC 2 cut(s) 224, 642
TfiI GAWTC 4 cut(s) 57, 125, 464, 482
Tru1I TTAA 3 cut(s) 216, 372, 546
Tru9I TTAA 3 cut(s) 216, 372, 546
TseI GCWGC 1 cut(s) 642
TspDTI ATGAA 2 cut(s) 158, 456
TspGWI ACGGA 2 cut(s) 569, 728
VpaK11BI GGWCC 4 cut(s) 98, 194, 382, 397
XbaI TCTAGA 1 cut(s) 167
XceI RCATGY 1 cut(s) 699
XmiI GTMKAC 1 cut(s) 648
XmnI GAANNNNTTC 2 cut(s) 159, 509
XspI CTAG 2 cut(s) 168, 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.