MD14G1030000.v1.1

Senescence regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0005726
Physical Location & Seq
Forward (+)
2597350 .. 2597964
615 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1030000.v1.1.491

Sequence Viewer

Length: 615 bp
ATGGATTCCACCAGCTTCTGCAACCAGCAATCGCCGTCGTCGGATCGGCTGCTCGGCCTCTTCTCCTTCTCTCCGCCCTCATCCGCCGCCGCCGGCGAGGAGCTCAACGAGGCCGAGGTCTTCTGGACCAACGACTTCACCGAGCCCACCGCCGACACCCCAAACCCCCGACTCAATCTGACTCGTCGCGTCCACTCCGGAATACTCGCGGTCCTCCCGGAACCGGGTCAATCGGCACAAGTCTTGTACCGGAAGCCGACAATTTCGTCATCGTCGAAGCCCATCCCTTCAATTCCGAGACTTTCGCCGTCCCAATTACAGTCCCAGTCGGTGCCTAATTCGAGGAAGATCCAGAGCTCGGCCCCGATGATGGTGCCGGTCCTGTCCCGGGCGGTGGAGAAGCACAGCAGAAACGATGATCTGGCGGATGTGGTGGACGACGACGACGGGGGAGACGATGAGATGCTGCCGCCGCACGAGCTCGTGGCGAGGGGGTCGGGGGTTTCGAATAGGACGACGTTCTCGGTGCTGGAAGGCGTGGGGAGGACGCTGAAAGGTAGGGATTTGCGTCAGGTTAGGAATGCGATTTGGCGCAAGACTGGGTTTCTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Senescence_reg

Protein Analysis

205

Amino Acids

22.24

Weight (kDa)

5.66

Isoelectric Point (pI)

53.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Senescence_reg PF04520 33 - 204 1.7e-42 Senescence regulator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 265
AccB1I GGYRCC 2 cut(s) 331, 373
AccII CGCG 2 cut(s) 189, 209
AccIII TCCGGA 1 cut(s) 197
AclWI GGATC 2 cut(s) 51, 343
AfaI GTAC 1 cut(s) 248
AfiI CCNNNNNNNGG 6 cut(s) 223, 224, 358, 370, 388, 394
AgsI TTSAA 1 cut(s) 291
AluBI AGCT 4 cut(s) 15, 103, 357, 481
AluI AGCT 4 cut(s) 15, 103, 357, 481
Alw21I GWGCWC 3 cut(s) 105, 359, 483
Alw26I GTCTC 2 cut(s) 292, 447
AlwI GGATC 2 cut(s) 51, 343
AlwNI CAGNNNCTG 1 cut(s) 18
Ama87I CYCGRG 1 cut(s) 387
Aor13HI TCCGGA 1 cut(s) 197
AoxI GGCC 3 cut(s) 55, 111, 360
ApeKI GCWGC 2 cut(s) 49, 466
AspLEI GCGC 1 cut(s) 594
AspS9I GGNCC 4 cut(s) 126, 211, 361, 379
AsuC2I CCSGG 4 cut(s) 218, 225, 388, 389
AsuHPI GGTGA 1 cut(s) 130
AsuII TTCGAA 1 cut(s) 506
AvaI CYCGRG 1 cut(s) 387
AvaII GGWCC 3 cut(s) 126, 211, 379
BanI GGYRCC 2 cut(s) 331, 373
BanII GRGCYC 4 cut(s) 105, 147, 359, 483
BauI CACGAG 2 cut(s) 476, 482
BbsI GAAGAC 1 cut(s) 112
Bbv12I GWGCWC 3 cut(s) 105, 359, 483
BbvI GCAGC 2 cut(s) 36, 453
BccI CCATC 2 cut(s) 290, 364
BceAI ACGGC 2 cut(s) 19, 292
BcgI CGANNNNNNTGC 2 cut(s) 355, 389
BcnI CCSGG 4 cut(s) 218, 225, 388, 389
BcoDI GTCTC 2 cut(s) 292, 447
BisI GCNGC 6 cut(s) 50, 87, 90, 467, 470, 473
BlsI GCNGC 6 cut(s) 51, 88, 91, 468, 471, 474
Bme1390I CCNGG 4 cut(s) 218, 225, 388, 389
Bme18I GGWCC 3 cut(s) 126, 211, 379
BmeT110I CYCGRG 1 cut(s) 387
BmgT120I GGNCC 4 cut(s) 126, 211, 361, 379
BmiI GGNNCC 4 cut(s) 222, 333, 363, 375
BmrFI CCNGG 4 cut(s) 218, 225, 388, 389
BmrI ACTGGG 2 cut(s) 319, 609
BmsI GCATC 1 cut(s) 453
BmuI ACTGGG 2 cut(s) 319, 609
BpiI GAAGAC 1 cut(s) 112
Bpu14I TTCGAA 1 cut(s) 506
BpuMI CCSGG 4 cut(s) 218, 225, 388, 389
BsaJI CCNNGG 2 cut(s) 114, 387
BsaWI WCCGGW 2 cut(s) 197, 249
Bsc4I CCNNNNNNNGG 6 cut(s) 223, 224, 358, 370, 388, 394
Bse118I RCCGGY 2 cut(s) 92, 376
Bse1I ACTGG 2 cut(s) 325, 604
BseAI TCCGGA 1 cut(s) 197
BseDI CCNNGG 2 cut(s) 114, 387
BseGI GGATG 3 cut(s) 80, 282, 433
BseLI CCNNNNNNNGG 6 cut(s) 223, 224, 358, 370, 388, 394
BseNI ACTGG 2 cut(s) 325, 604
BseRI GAGGAG 1 cut(s) 113
BseXI GCAGC 2 cut(s) 36, 453
Bsh1236I CGCG 2 cut(s) 189, 209
BshFI GGCC 3 cut(s) 57, 113, 362
BshNI GGYRCC 2 cut(s) 331, 373
BsiHKAI GWGCWC 3 cut(s) 105, 359, 483
BsiHKCI CYCGRG 1 cut(s) 387
BsiSI CCGG 7 cut(s) 93, 198, 218, 224, 250, 377, 388
BslFI GGGAC 3 cut(s) 295, 307, 370
BslI CCNNNNNNNGG 6 cut(s) 223, 224, 358, 370, 388, 394
BsmAI GTCTC 2 cut(s) 292, 447
BsmBI CGTCTC 1 cut(s) 447
BsmFI GGGAC 3 cut(s) 295, 307, 370
BsmI GAATGC 1 cut(s) 586
BsnI GGCC 3 cut(s) 57, 113, 362
BsoBI CYCGRG 1 cut(s) 387
Bsp119I TTCGAA 1 cut(s) 506
Bsp1286I GDGCHC 4 cut(s) 105, 147, 359, 483
Bsp13I TCCGGA 1 cut(s) 197
Bsp143I GATC 3 cut(s) 43, 348, 418
BspANI GGCC 3 cut(s) 57, 113, 362
BspEI TCCGGA 1 cut(s) 197
BspFNI CGCG 2 cut(s) 189, 209
BspLI GGNNCC 4 cut(s) 222, 333, 363, 375
BspPI GGATC 2 cut(s) 51, 343
BspT104I TTCGAA 1 cut(s) 506
BspT107I GGYRCC 2 cut(s) 331, 373
BsrFI RCCGGY 2 cut(s) 92, 376
BsrI ACTGG 2 cut(s) 325, 604
BssAI RCCGGY 2 cut(s) 92, 376
BssECI CCNNGG 2 cut(s) 114, 387
BssMI GATC 3 cut(s) 43, 348, 418
BssSI CACGAG 2 cut(s) 476, 482
Bst2BI CACGAG 2 cut(s) 476, 482
Bst4CI ACNGT 1 cut(s) 321
Bst6I CTCTTC 1 cut(s) 65
BstBI TTCGAA 1 cut(s) 506
BstC8I GCNNGC 1 cut(s) 94
BstF5I GGATG 3 cut(s) 80, 282, 433
BstFNI CGCG 2 cut(s) 189, 209
BstHHI GCGC 1 cut(s) 594
BstKTI GATC 3 cut(s) 46, 351, 421
BstMAI GTCTC 2 cut(s) 292, 447
BstMBI GATC 3 cut(s) 43, 348, 418
BstMWI GCNNNNNNNGC 2 cut(s) 472, 478
BstSCI CCNGG 4 cut(s) 216, 223, 386, 387
BstUI CGCG 2 cut(s) 189, 209
BstV1I GCAGC 2 cut(s) 36, 453
BstV2I GAAGAC 1 cut(s) 112
BstX2I RGATCY 1 cut(s) 348
BstYI RGATCY 1 cut(s) 348
BsuRI GGCC 3 cut(s) 57, 113, 362
BtsCI GGATG 3 cut(s) 80, 282, 433
Cac8I GCNNGC 1 cut(s) 94
CaiI CAGNNNCTG 1 cut(s) 18
CfoI GCGC 1 cut(s) 594
Cfr10I RCCGGY 2 cut(s) 92, 376
Cfr13I GGNCC 4 cut(s) 126, 211, 361, 379
Cfr9I CCCGGG 1 cut(s) 387
CseI GACGC 3 cut(s) 178, 556, 557
Csp6I GTAC 1 cut(s) 247
CviQI GTAC 1 cut(s) 247
DpnI GATC 3 cut(s) 45, 350, 420
DpnII GATC 3 cut(s) 43, 348, 418
DrdI GACNNNNNNGTC 1 cut(s) 265
DseDI GACNNNNNNGTC 1 cut(s) 265
Eam1104I CTCTTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 65
EciI GGCGGA 3 cut(s) 63, 73, 440
Ecl136II GAGCTC 3 cut(s) 103, 357, 481
Eco24I GRGCYC 4 cut(s) 105, 147, 359, 483
Eco47I GGWCC 3 cut(s) 126, 211, 379
Eco53kI GAGCTC 3 cut(s) 103, 357, 481
Eco88I CYCGRG 1 cut(s) 387
EcoICRI GAGCTC 3 cut(s) 103, 357, 481
EcoT38I GRGCYC 4 cut(s) 105, 147, 359, 483
Esp3I CGTCTC 1 cut(s) 447
FaqI GGGAC 3 cut(s) 295, 307, 370
Fnu4HI GCNGC 6 cut(s) 50, 87, 90, 467, 470, 473
FokI GGATG 3 cut(s) 67, 269, 440
FriOI GRGCYC 4 cut(s) 105, 147, 359, 483
Fsp4HI GCNGC 6 cut(s) 50, 87, 90, 467, 470, 473
GlaI GCGC 1 cut(s) 593
GluI GCNGC 6 cut(s) 50, 87, 90, 467, 470, 473
HaeIII GGCC 3 cut(s) 57, 113, 362
HapII CCGG 7 cut(s) 93, 198, 218, 224, 250, 377, 388
HgaI GACGC 3 cut(s) 178, 556, 557
HhaI GCGC 1 cut(s) 594
Hin6I GCGC 1 cut(s) 592
HinP1I GCGC 1 cut(s) 592
HinfI GANTC 3 cut(s) 5, 171, 181
HpaII CCGG 7 cut(s) 93, 198, 218, 224, 250, 377, 388
HphI GGTGA 1 cut(s) 130
Hpy166II GTNNAC 2 cut(s) 193, 436
Hpy188I TCNGA 3 cut(s) 43, 180, 297
Hpy188III TCNNGA 4 cut(s) 124, 198, 352, 608
Hpy8I GTNNAC 2 cut(s) 193, 436
Hpy99I CGWCG 8 cut(s) 40, 43, 189, 277, 443, 446, 449, 520
HpyAV CCTTC 3 cut(s) 76, 297, 527
HpyCH4III ACNGT 1 cut(s) 321
HpyCH4IV ACGT 1 cut(s) 518
HpyCH4V TGCA 1 cut(s) 21
HpyF10VI GCNNNNNNNGC 2 cut(s) 472, 478
HpySE526I ACGT 1 cut(s) 518
HspAI GCGC 1 cut(s) 592
Kpn2I TCCGGA 1 cut(s) 197
KroI GCCGGC 1 cut(s) 92
KroNI GCCGGC 1 cut(s) 94
Kzo9I GATC 3 cut(s) 43, 348, 418
LmnI GCTCC 1 cut(s) 100
Lsp1109I GCAGC 2 cut(s) 36, 453
LweI GCATC 1 cut(s) 453
MaeII ACGT 1 cut(s) 518
MalI GATC 3 cut(s) 45, 350, 420
MboI GATC 3 cut(s) 43, 348, 418
MboII GAAGA 3 cut(s) 52, 112, 358
MflI RGATCY 1 cut(s) 348
MhlI GDGCHC 4 cut(s) 105, 147, 359, 483
MluCI AATT 4 cut(s) 261, 291, 314, 337
MlyI GAGTC 2 cut(s) 165, 175
MmeI TCCRAC 1 cut(s) 21
MnlI CCTC 9 cut(s) 68, 88, 91, 103, 109, 224, 336, 483, 537
MreI CGCCGGCG 1 cut(s) 92
MroI TCCGGA 1 cut(s) 197
MroNI GCCGGC 1 cut(s) 92
MspI CCGG 7 cut(s) 93, 198, 218, 224, 250, 377, 388
MspR9I CCNGG 4 cut(s) 218, 225, 388, 389
Mva1269I GAATGC 1 cut(s) 586
MvnI CGCG 2 cut(s) 189, 209
MwoI GCNNNNNNNGC 2 cut(s) 472, 478
NaeI GCCGGC 1 cut(s) 94
NciI CCSGG 4 cut(s) 218, 225, 388, 389
NdeII GATC 3 cut(s) 43, 348, 418
NgoMIV GCCGGC 1 cut(s) 92
NlaIV GGNNCC 4 cut(s) 222, 333, 363, 375
NmeAIII GCCGAG 3 cut(s) 33, 139, 338
NspV TTCGAA 1 cut(s) 506
PcsI WCGNNNNNNNCGW 5 cut(s) 138, 272, 453, 503, 512
PctI GAATGC 1 cut(s) 586
PdiI GCCGGC 1 cut(s) 94
PfeI GAWTC 1 cut(s) 5
PfoI TCCNGGA 1 cut(s) 216
PkrI GCNGC 6 cut(s) 51, 88, 91, 468, 471, 474
PleI GAGTC 2 cut(s) 165, 175
PpsI GAGTC 2 cut(s) 165, 175
Psp124BI GAGCTC 3 cut(s) 105, 359, 483
PspN4I GGNNCC 4 cut(s) 222, 333, 363, 375
PspPI GGNCC 4 cut(s) 126, 211, 361, 379
PstNI CAGNNNCTG 1 cut(s) 18
PsuI RGATCY 1 cut(s) 348
RsaI GTAC 1 cut(s) 248
RsaNI GTAC 1 cut(s) 247
SacI GAGCTC 3 cut(s) 105, 359, 483
SatI GCNGC 6 cut(s) 50, 87, 90, 467, 470, 473
Sau3AI GATC 3 cut(s) 43, 348, 418
Sau96I GGNCC 4 cut(s) 126, 211, 361, 379
SchI GAGTC 2 cut(s) 165, 175
ScrFI CCNGG 4 cut(s) 218, 225, 388, 389
SduI GDGCHC 4 cut(s) 105, 147, 359, 483
SetI ASST 8 cut(s) 17, 105, 120, 359, 483, 521, 559, 576
SfaNI GCATC 1 cut(s) 453
SfuI TTCGAA 1 cut(s) 506
SgrAI CRCCGGYG 1 cut(s) 92
SinI GGWCC 3 cut(s) 126, 211, 379
SmaI CCCGGG 1 cut(s) 389
Sse9I AATT 4 cut(s) 261, 291, 314, 337
SstI GAGCTC 3 cut(s) 105, 359, 483
StyD4I CCNGG 4 cut(s) 216, 223, 386, 387
TaaI ACNGT 1 cut(s) 321
TaiI ACGT 1 cut(s) 521
TaqI TCGA 3 cut(s) 275, 341, 506
TasI AATT 4 cut(s) 261, 291, 314, 337
TauI GCSGC 4 cut(s) 89, 92, 472, 475
TfiI GAWTC 1 cut(s) 5
TseI GCWGC 2 cut(s) 49, 466
TspMI CCCGGG 1 cut(s) 387
VpaK11BI GGWCC 3 cut(s) 126, 211, 379
XmaI CCCGGG 1 cut(s) 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.