MD15G1002200.v1.1

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005901
Physical Location & Seq
Reverse (-)
128420 .. 129925
1506 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1002200.v1.1.491

Sequence Viewer

Length: 1506 bp
ATGTCGACGTCCAAGATGCAAGTCAAGATAATTTCCGATGAGTGCATCAAACCCTCTTCCCCAACACCCCAACACCTGAGAACCCACAAGCTCTCCCTTTTAGACCAGTTCATTCCCTCCATCTATGTACCCATGATCCTCTTCTTTAACCCCTCGTCCTCCATTCACCCCATTAATATTAATATTACTACTCCAATGAGGTCCCAACTGCTAAAGCAATCCCTCTCAGAAACCTTGACTCACTTTCACCCACTTGCCGGTAGAATTAGAGATAGTCTTTCTATTGATTGCAATGACGAGCGGGCTCGTTACATAGAGGCCGAAGTTGACTCCAATCTCTCCCAGTTTCTCAACCATCCTGATCTCTCATTCATCAGTCAGTTCCTTCCCGGTGAGGCTAGTTGGCACGAAGCAGCTCCCGGAGCTCATGTAGCTATGATGCAAATCACCAACTTTGCATGCGGCGGTTTCGTTCTGGGTGCCTTTGTCTCACATATGGTTGTTGATGGAACCGCTTTGAGCGCCTTCTTCAAAGCTTGGGCCGCTGCAACATTTCGCTACATAGCGACTCCTAACACTCCTCATTATATTTATACCAAGGGTAAGTTTGATGCACCATCTTTGTTTCCCCAAAATGATGCATACCCAAGAGAAGCGACTATGATGGCTTTGTACTACCGCTTCCTGAAAGTGGGAAACTGTGTTACAAGGAGAATTGTATTTGATGCTCCTGCTCTGGCCACACTCAAGAGAAAAGCAGCCAGCTCAACGGTGCCAAATCCAACGCGTGTGGAGGTTGTCACGGCACTCATCTGGAAGCACATTATGGCTGCCTTTAACTTGAAGACCGAACGTAATATTGTTCAGAACCGAAGCACAACTCTGATAACACACGCTGTGAATTTGCGTCGAAGAGCTGTGCCACCATTTTCCGAATACACTATGGGAAATTTTCTGTCCATGGCAGCCGCACTGTCACAATGCAGTACTGAAGATAATTATCAAAAGGAGGACAACTTAGCTCATGTTGATGATGATCGTGATCATGAGTTGGGTAGGTTGGCGAAGCATGTGAGGGAAGCAATAAGCAAACTTGATGGTGATTTTGTACAAAGCCTACAAGGAGATGGGACTAGAGGGCTCACCAAATTCTGTGAGCCTTTCAAGGAGATAAGAGAGACTTTGTCTCGCACAAGTGCAGATGCAGCTGATGGACAGAGCCTGGATTGTATAGGGTTTAGCAGCTGGTGCAATTTTGGTCTTTATGAAGTTGATTTTGGGTGGGGAAAGCCGTCGTGGGTGTCATGTGTTGGAGGAAAGAGGGGTGCAGATGAGCCTGTGTACATGAATATGGTGGTTTTAATGGATACAAGGTCGGGGGATGGGGTTGAAGCCTGGATTTATCTAGATGAGCAAGACATTGCTATATTAGAGCATGATCCTCACTTCCTTGCTTTTGTTTCTTTAAACCCTAGTCCTCTTTCTGTTGCCATCAAAACACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transferase

Protein Analysis

502

Amino Acids

55.85

Weight (kDa)

6.28

Isoelectric Point (pI)

40.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 6 - 482 3e-76 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 11
AccB1I GGYRCC 2 cut(s) 479, 772
AccBSI CCGCTC 1 cut(s) 301
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 787
AciI CCGC 7 cut(s) 301, 462, 465, 513, 543, 679, 969
AclWI GGATC 2 cut(s) 130, 1433
AcoI YGGCCR 1 cut(s) 738
AcsI RAATTY 3 cut(s) 901, 949, 1148
AcuI CTGAAG 1 cut(s) 1011
AcyI GRCGYC 1 cut(s) 8
AdeI CACNNNGTG 1 cut(s) 898
AfaI GTAC 5 cut(s) 129, 674, 988, 1110, 1343
AfiI CCNNNNNNNGG 2 cut(s) 257, 691
AflIII ACRYGT 1 cut(s) 785
AgsI TTSAA 4 cut(s) 532, 844, 1165, 1391
AjnI CCWGG 2 cut(s) 1221, 1394
AjuI GAANNNNNNNTTGG 2 cut(s) 1260, 1292
Alw21I GWGCWC 1 cut(s) 427
Alw26I GTCTC 3 cut(s) 493, 1172, 1191
AlwI GGATC 2 cut(s) 130, 1433
AlwNI CAGNNNCTG 1 cut(s) 1222
AoxI GGCC 3 cut(s) 318, 540, 738
ApeKI GCWGC 7 cut(s) 413, 545, 758, 830, 965, 1205, 1242
ApoI RAATTY 3 cut(s) 901, 949, 1148
AseI ATTAAT 2 cut(s) 174, 180
AspLEI GCGC 1 cut(s) 524
AspS9I GGNCC 2 cut(s) 201, 540
AsuC2I CCSGG 2 cut(s) 390, 420
AsuHPI GGTGA 6 cut(s) 158, 239, 404, 439, 1112, 1135
AvaII GGWCC 1 cut(s) 201
BalI TGGCCA 1 cut(s) 740
BanI GGYRCC 2 cut(s) 479, 772
BanII GRGCYC 3 cut(s) 307, 427, 1143
BarI GAAGNNNNNNTAC 2 cut(s) 665, 697
BbsI GAAGAC 1 cut(s) 851
Bbv12I GWGCWC 1 cut(s) 427
BbvI GCAGC 7 cut(s) 425, 532, 770, 817, 977, 1217, 1254
BceAI ACGGC 2 cut(s) 819, 1276
BciT130I CCWGG 2 cut(s) 1223, 1396
BciVI GTATCC 1 cut(s) 1360
BclI TGATCA 1 cut(s) 1042
BcnI CCSGG 2 cut(s) 390, 420
BcoDI GTCTC 3 cut(s) 493, 1172, 1191
BfaI CTAG 4 cut(s) 399, 1134, 1406, 1473
BfoI RGCGCY 1 cut(s) 525
BfuI GTATCC 1 cut(s) 1360
BmcAI AGTACT 1 cut(s) 988
Bme1390I CCNGG 4 cut(s) 390, 420, 1223, 1396
Bme18I GGWCC 1 cut(s) 201
BmgT120I GGNCC 2 cut(s) 201, 540
BmiI GGNNCC 4 cut(s) 203, 481, 511, 774
BmrFI CCNGG 4 cut(s) 390, 420, 1223, 1396
BmrI ACTGGG 1 cut(s) 337
BmsI GCATC 7 cut(s) 6, 54, 429, 601, 628, 715, 1192
BmuI ACTGGG 1 cut(s) 337
BpiI GAAGAC 1 cut(s) 851
BpuEI CTTGAG 1 cut(s) 731
BpuMI CCSGG 2 cut(s) 390, 420
BsaBI GATNNNNATC 4 cut(s) 443, 999, 1035, 1041
BsaHI GRCGYC 1 cut(s) 8
BsaJI CCNNGG 2 cut(s) 597, 960
BsaXI ACNNNNNCTCC 4 cut(s) 77, 107, 414, 444
Bsc4I CCNNNNNNNGG 2 cut(s) 257, 691
Bse118I RCCGGY 1 cut(s) 257
Bse1I ACTGG 2 cut(s) 106, 343
Bse3DI GCAATG 2 cut(s) 298, 1419
Bse8I GATNNNNATC 4 cut(s) 443, 999, 1035, 1041
BseBI CCWGG 2 cut(s) 1223, 1396
BseDI CCNNGG 2 cut(s) 597, 960
BseGI GGATG 2 cut(s) 355, 1387
BseJI GATNNNNATC 4 cut(s) 443, 999, 1035, 1041
BseLI CCNNNNNNNGG 2 cut(s) 257, 691
BseMI GCAATG 2 cut(s) 298, 1419
BseMII CTCAG 2 cut(s) 68, 240
BseNI ACTGG 2 cut(s) 106, 343
BseRI GAGGAG 1 cut(s) 570
BseXI GCAGC 7 cut(s) 425, 532, 770, 817, 977, 1217, 1254
BsgI GTGCAG 2 cut(s) 1218, 1347
Bsh1236I CGCG 1 cut(s) 787
BshFI GGCC 3 cut(s) 320, 542, 740
BshNI GGYRCC 2 cut(s) 479, 772
BsiHKAI GWGCWC 1 cut(s) 427
BsiSI CCGG 3 cut(s) 258, 390, 420
BslFI GGGAC 2 cut(s) 187, 1144
BslI CCNNNNNNNGG 2 cut(s) 257, 691
BsmAI GTCTC 3 cut(s) 493, 1172, 1191
BsmFI GGGAC 2 cut(s) 187, 1144
BsnI GGCC 3 cut(s) 320, 542, 740
Bsp1286I GDGCHC 3 cut(s) 307, 427, 1143
Bsp1407I TGTACA 2 cut(s) 1108, 1341
Bsp143I GATC 5 cut(s) 135, 361, 1036, 1042, 1438
Bsp19I CCATGG 1 cut(s) 960
BspACI CCGC 7 cut(s) 301, 462, 465, 513, 543, 679, 969
BspANI GGCC 3 cut(s) 320, 542, 740
BspCNI CTCAG 2 cut(s) 69, 239
BspFNI CGCG 1 cut(s) 787
BspHI TCATGA 1 cut(s) 1045
BspLI GGNNCC 4 cut(s) 203, 481, 511, 774
BspPI GGATC 2 cut(s) 130, 1433
BspQI GCTCTTC 1 cut(s) 907
BspT107I GGYRCC 2 cut(s) 479, 772
BsrBI CCGCTC 1 cut(s) 301
BsrDI GCAATG 2 cut(s) 298, 1419
BsrFI RCCGGY 1 cut(s) 257
BsrGI TGTACA 2 cut(s) 1108, 1341
BsrI ACTGG 2 cut(s) 106, 343
BssAI RCCGGY 1 cut(s) 257
BssECI CCNNGG 2 cut(s) 597, 960
BssMI GATC 5 cut(s) 135, 361, 1036, 1042, 1438
BssNI GRCGYC 1 cut(s) 8
BssT1I CCWWGG 2 cut(s) 597, 960
Bst2UI CCWGG 2 cut(s) 1223, 1396
Bst4CI ACNGT 3 cut(s) 701, 772, 975
Bst6I CTCTTC 3 cut(s) 61, 146, 907
BstACI GRCGYC 1 cut(s) 8
BstAPI GCANNNNNTGC 1 cut(s) 1248
BstAUI TGTACA 2 cut(s) 1108, 1341
BstC8I GCNNGC 3 cut(s) 303, 460, 763
BstDEI CTNAG 3 cut(s) 77, 226, 1018
BstDSI CCRYGG 1 cut(s) 960
BstF5I GGATG 2 cut(s) 355, 1387
BstFNI CGCG 1 cut(s) 787
BstH2I RGCGCY 1 cut(s) 525
BstHHI GCGC 1 cut(s) 524
BstKTI GATC 5 cut(s) 138, 364, 1039, 1045, 1441
BstMAI GTCTC 3 cut(s) 493, 1172, 1191
BstMBI GATC 5 cut(s) 135, 361, 1036, 1042, 1438
BstMWI GCNNNNNNNGC 6 cut(s) 422, 431, 521, 542, 1205, 1248
BstNI CCWGG 2 cut(s) 1223, 1396
BstNSI RCATGY 2 cut(s) 462, 1073
BstSCI CCNGG 4 cut(s) 388, 418, 1221, 1394
BstUI CGCG 1 cut(s) 787
BstV1I GCAGC 7 cut(s) 425, 532, 770, 817, 977, 1217, 1254
BstV2I GAAGAC 1 cut(s) 851
BsuI GTATCC 1 cut(s) 1360
BsuRI GGCC 3 cut(s) 320, 542, 740
BtgI CCRYGG 1 cut(s) 960
BtsCI GGATG 2 cut(s) 355, 1387
BtsIMutI CAGTG 1 cut(s) 971
Cac8I GCNNGC 3 cut(s) 303, 460, 763
CaiI CAGNNNCTG 1 cut(s) 1222
CciI TCATGA 1 cut(s) 1045
CfoI GCGC 1 cut(s) 524
Cfr10I RCCGGY 1 cut(s) 257
Cfr13I GGNCC 2 cut(s) 201, 540
CseI GACGC 1 cut(s) 896
Csp6I GTAC 5 cut(s) 128, 673, 987, 1109, 1342
CspCI CAANNNNNGTGG 2 cut(s) 771, 806
CviQI GTAC 5 cut(s) 128, 673, 987, 1109, 1342
DdeI CTNAG 3 cut(s) 77, 226, 1018
DpnI GATC 5 cut(s) 137, 363, 1038, 1044, 1440
DpnII GATC 5 cut(s) 135, 361, 1036, 1042, 1438
DraI TTTAAA 1 cut(s) 1467
DraIII CACNNNGTG 1 cut(s) 898
EaeI YGGCCR 1 cut(s) 738
Eam1104I CTCTTC 3 cut(s) 61, 146, 907
EarI CTCTTC 3 cut(s) 61, 146, 907
Ecl136II GAGCTC 1 cut(s) 425
Eco130I CCWWGG 2 cut(s) 597, 960
Eco24I GRGCYC 3 cut(s) 307, 427, 1143
Eco47I GGWCC 1 cut(s) 201
Eco53kI GAGCTC 1 cut(s) 425
Eco57I CTGAAG 1 cut(s) 1011
EcoICRI GAGCTC 1 cut(s) 425
EcoO109I RGGNCCY 1 cut(s) 201
EcoRII CCWGG 2 cut(s) 1221, 1394
EcoT14I CCWWGG 2 cut(s) 597, 960
EcoT22I ATGCAT 1 cut(s) 643
EcoT38I GRGCYC 3 cut(s) 307, 427, 1143
ErhI CCWWGG 2 cut(s) 597, 960
FalI AAGNNNNNCTT 2 cut(s) 1165, 1197
FaqI GGGAC 2 cut(s) 187, 1144
FauI CCCGC 1 cut(s) 294
FauNDI CATATG 1 cut(s) 495
FbaI TGATCA 1 cut(s) 1042
FblI GTMKAC 1 cut(s) 5
FokI GGATG 2 cut(s) 342, 1394
FriOI GRGCYC 3 cut(s) 307, 427, 1143
FspBI CTAG 4 cut(s) 399, 1134, 1406, 1473
GlaI GCGC 1 cut(s) 523
HaeII RGCGCY 1 cut(s) 525
HaeIII GGCC 3 cut(s) 320, 542, 740
HapII CCGG 3 cut(s) 258, 390, 420
HgaI GACGC 1 cut(s) 896
HhaI GCGC 1 cut(s) 524
Hin1I GRCGYC 1 cut(s) 8
Hin6I GCGC 1 cut(s) 522
HinP1I GCGC 1 cut(s) 522
HincII GTYRAC 2 cut(s) 6, 328
HindII GTYRAC 2 cut(s) 6, 328
HindIII AAGCTT 1 cut(s) 534
HinfI GANTC 3 cut(s) 238, 329, 568
HpaII CCGG 3 cut(s) 258, 390, 420
HphI GGTGA 6 cut(s) 158, 239, 404, 439, 1112, 1135
Hpy166II GTNNAC 3 cut(s) 6, 328, 1342
Hpy188I TCNGA 5 cut(s) 37, 229, 867, 885, 934
Hpy188III TCNNGA 8 cut(s) 25, 359, 685, 748, 814, 1040, 1046, 1406
Hpy8I GTNNAC 3 cut(s) 6, 328, 1342
Hpy99I CGWCG 3 cut(s) 10, 912, 1297
HpyAV CCTTC 2 cut(s) 395, 535
HpyCH4III ACNGT 3 cut(s) 701, 772, 975
HpyCH4IV ACGT 2 cut(s) 8, 853
HpyF10VI GCNNNNNNNGC 6 cut(s) 422, 431, 521, 542, 1205, 1248
HpyF3I CTNAG 3 cut(s) 77, 226, 1018
HpySE526I ACGT 2 cut(s) 8, 853
Hsp92I GRCGYC 1 cut(s) 8
HspAI GCGC 1 cut(s) 522
Ksp22I TGATCA 1 cut(s) 1042
Kzo9I GATC 5 cut(s) 135, 361, 1036, 1042, 1438
LguI GCTCTTC 1 cut(s) 907
LmnI GCTCC 3 cut(s) 421, 422, 733
Lsp1109I GCAGC 7 cut(s) 425, 532, 770, 817, 977, 1217, 1254
LweI GCATC 7 cut(s) 6, 54, 429, 601, 628, 715, 1192
MaeI CTAG 4 cut(s) 399, 1134, 1406, 1473
MaeII ACGT 2 cut(s) 8, 853
MaeIII GTNAC 4 cut(s) 308, 703, 799, 975
MalI GATC 5 cut(s) 137, 363, 1038, 1044, 1440
MbiI CCGCTC 1 cut(s) 301
MboI GATC 5 cut(s) 135, 361, 1036, 1042, 1438
MboII GAAGA 6 cut(s) 48, 133, 520, 856, 924, 1004
MhlI GDGCHC 3 cut(s) 307, 427, 1143
MlsI TGGCCA 1 cut(s) 740
MluCI AATT 8 cut(s) 30, 264, 714, 901, 949, 997, 1148, 1252
MluI ACGCGT 1 cut(s) 785
MluNI TGGCCA 1 cut(s) 740
MlyI GAGTC 3 cut(s) 232, 323, 562
MmeI TCCRAC 2 cut(s) 806, 1291
Mox20I TGGCCA 1 cut(s) 740
Mph1103I ATGCAT 1 cut(s) 643
MscI TGGCCA 1 cut(s) 740
MseI TTAA 6 cut(s) 147, 174, 180, 837, 1361, 1466
MslI CAYNNNNRTG 2 cut(s) 1029, 1349
Msp20I TGGCCA 1 cut(s) 740
MspA1I CMGCKG 3 cut(s) 545, 1208, 1245
MspI CCGG 3 cut(s) 258, 390, 420
MspR9I CCNGG 4 cut(s) 390, 420, 1223, 1396
MvaI CCWGG 2 cut(s) 1223, 1396
MvnI CGCG 1 cut(s) 787
MwoI GCNNNNNNNGC 6 cut(s) 422, 431, 521, 542, 1205, 1248
NciI CCSGG 2 cut(s) 390, 420
NcoI CCATGG 1 cut(s) 960
NdeI CATATG 1 cut(s) 495
NdeII GATC 5 cut(s) 135, 361, 1036, 1042, 1438
NlaIV GGNNCC 4 cut(s) 203, 481, 511, 774
NmuCI GTSAC 2 cut(s) 799, 975
NsiI ATGCAT 1 cut(s) 643
NspI RCATGY 2 cut(s) 462, 1073
PaeI GCATGC 1 cut(s) 462
PagI TCATGA 1 cut(s) 1045
PciSI GCTCTTC 1 cut(s) 907
PflFI GACNNNGTC 1 cut(s) 1183
PfoI TCCNGGA 1 cut(s) 418
PleI GAGTC 3 cut(s) 232, 323, 562
PpsI GAGTC 3 cut(s) 232, 323, 562
PpuMI RGGWCCY 1 cut(s) 201
PshBI ATTAAT 2 cut(s) 174, 180
Psp124BI GAGCTC 1 cut(s) 427
Psp5II RGGWCCY 1 cut(s) 201
Psp6I CCWGG 2 cut(s) 1221, 1394
PspGI CCWGG 2 cut(s) 1221, 1394
PspN4I GGNNCC 4 cut(s) 203, 481, 511, 774
PspPI GGNCC 2 cut(s) 201, 540
PspPPI RGGWCCY 1 cut(s) 201
PstNI CAGNNNCTG 1 cut(s) 1222
PsyI GACNNNGTC 1 cut(s) 1183
PvuII CAGCTG 2 cut(s) 1208, 1245
RsaI GTAC 5 cut(s) 129, 674, 988, 1110, 1343
RsaNI GTAC 5 cut(s) 128, 673, 987, 1109, 1342
RseI CAYNNNNRTG 2 cut(s) 1029, 1349
SacI GAGCTC 1 cut(s) 427
SalI GTCGAC 1 cut(s) 4
SapI GCTCTTC 1 cut(s) 907
SaqAI TTAA 6 cut(s) 147, 174, 180, 837, 1361, 1466
Sau3AI GATC 5 cut(s) 135, 361, 1036, 1042, 1438
Sau96I GGNCC 2 cut(s) 201, 540
ScaI AGTACT 1 cut(s) 988
SchI GAGTC 3 cut(s) 232, 323, 562
ScrFI CCNGG 4 cut(s) 390, 420, 1223, 1396
SduI GDGCHC 3 cut(s) 307, 427, 1143
SfaNI GCATC 7 cut(s) 6, 54, 429, 601, 628, 715, 1192
SinI GGWCC 1 cut(s) 201
SmiMI CAYNNNNRTG 2 cut(s) 1029, 1349
SmlI CTYRAG 1 cut(s) 746
SmoI CTYRAG 1 cut(s) 746
SphI GCATGC 1 cut(s) 462
Sse9I AATT 8 cut(s) 30, 264, 714, 901, 949, 997, 1148, 1252
SsiI CCGC 7 cut(s) 301, 462, 465, 513, 543, 679, 969
SspI AATATT 3 cut(s) 178, 184, 859
SspMI CTAG 4 cut(s) 399, 1134, 1406, 1473
SstI GAGCTC 1 cut(s) 427
StyD4I CCNGG 4 cut(s) 388, 418, 1221, 1394
StyI CCWWGG 2 cut(s) 597, 960
TaaI ACNGT 3 cut(s) 701, 772, 975
TaiI ACGT 2 cut(s) 11, 856
TaqI TCGA 2 cut(s) 5, 910
TaqII GACCGA 1 cut(s) 863
TasI AATT 8 cut(s) 30, 264, 714, 901, 949, 997, 1148, 1252
TatI WGTACW 4 cut(s) 672, 986, 1108, 1341
TauI GCSGC 3 cut(s) 465, 545, 971
Tru1I TTAA 6 cut(s) 147, 174, 180, 837, 1361, 1466
Tru9I TTAA 6 cut(s) 147, 174, 180, 837, 1361, 1466
TscAI CASTG 1 cut(s) 978
TseFI GTSAC 2 cut(s) 799, 975
TseI GCWGC 7 cut(s) 413, 545, 758, 830, 965, 1205, 1242
Tsp45I GTSAC 2 cut(s) 799, 975
TspDTI ATGAA 4 cut(s) 100, 361, 1281, 1361
TspRI CASTG 1 cut(s) 978
Tth111I GACNNNGTC 1 cut(s) 1183
VpaK11BI GGWCC 1 cut(s) 201
VspI ATTAAT 2 cut(s) 174, 180
XapI RAATTY 3 cut(s) 901, 949, 1148
XbaI TCTAGA 1 cut(s) 1405
XceI RCATGY 2 cut(s) 462, 1073
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 4 cut(s) 399, 1134, 1406, 1473
ZraI GACGTC 1 cut(s) 9
ZrmI AGTACT 1 cut(s) 988
Zsp2I ATGCAT 1 cut(s) 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.