MD15G1032200.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005177
Physical Location & Seq
Forward (+)
1983295 .. 1985648
2354 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1032200.v1.1.491

Sequence Viewer

Length: 1077 bp
ATGTATCCGCAATCAGCTGCCCTGCCTGCTGATATCAGCTTACAGTGTACCGATGAGGAGCTCTGTGTGTCCTTAGAGAAAATACAGGACGGATCCCCGCTTCCTGGAAATGTGATTCGAGATGTAAATCCTTACCGATGCGCGCCCTCGAATTTACCCGATGGTTTTTGGTACTTCATTCGCTCGAGTGAGAGCAACCCTACAAATATTGGGCAGTGGAGAGCCAAAGGGGAGGCCAGTAGAATATTCTCAAACTCCTCCATCTATGGTTGGAGAGCTACACTTGAATTCTATCGAGGCCAAGCCCCTCATGAAACTAAAACCAATTGGGTGATGCAAGAATACTGGATAACTCAGAAGAAATCGTCGGAAAGCAGCAAATTGAAGATAAATGTGCAGGATGTCGGGTCGCTTTGCAGAGTCTTTCTTAGGGATGATGAAACACCAAACTCCAATAAGCTTCAGGAATTGGTGAATTCTGATAGGGCTATTCACTCAACACATTCAGTTGTTCCGCGTGTTGAGGATCGTGGTTCCACCAGCAAGCCTCGTGTTGAAAATGCAAGCAATGATCGCGAAACGGGAACATTGGTAGTTACAGAAAGGCTTCCAGTTCGAGATGATAATGTGCAAATTATGCACGAGAATGATTACCTTTCGAGAGGCGACTACTTGGAATTGCTGGATCTTGACACACCAGCATCTCATTCATTTAGTTCAGTTTCGAGTTGCTTGACAATGTCCTCGGACGAATGCTTCGATTCTCTGGCATTGTTGGAAGAACTAGAGCCTAAAAGTAAGCAAGAATTGGTGACGAACAACAATAAGTTGGTCGTGTTTGCATCCTCTTCAGGATCTTTCGACAAGTCTCGCGACAAAGAAAGAATCAGAAATCGTTCTCCGAATCCTGGCTCATCTGTTTGTGCCAAGATTGTCACAGAAAATAGTTCAAAAAATGCGAGCAGAAAACAAAAATCAGACGATAGGAATGAAGGGCCATCAAATTCTCAGAATGCTAATGTTGGTGGAACCAAAAAGCTTAAGAGGAAGAAGTATTTCTGCTTCATGCCGTTTTAG

Protein Analysis

359

Amino Acids

40.24

Weight (kDa)

6.56

Isoelectric Point (pI)

47.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 13 - 115 7.8e-16 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 143, 517, 576, 873
AciI CCGC 3 cut(s) 8, 98, 515
AclWI GGATC 5 cut(s) 87, 100, 534, 693, 862
AcsI RAATTY 4 cut(s) 151, 287, 475, 1003
AcuI CTGAAG 2 cut(s) 446, 834
AfaI GTAC 2 cut(s) 49, 173
AfiI CCNNNNNNNGG 2 cut(s) 104, 908
AflII CTTAAG 1 cut(s) 1040
AgsI TTSAA 4 cut(s) 287, 385, 557, 951
AjnI CCWGG 2 cut(s) 103, 907
AluBI AGCT 6 cut(s) 17, 39, 61, 278, 460, 1039
AluI AGCT 6 cut(s) 17, 39, 61, 278, 460, 1039
Alw21I GWGCWC 1 cut(s) 63
Alw26I GTCTC 1 cut(s) 873
AlwI GGATC 5 cut(s) 87, 100, 534, 693, 862
Ama87I CYCGRG 1 cut(s) 184
AoxI GGCC 3 cut(s) 234, 298, 995
ApeKI GCWGC 2 cut(s) 17, 375
ApoI RAATTY 4 cut(s) 151, 287, 475, 1003
ArsI GACNNNNNNTTYG 2 cut(s) 740, 772
Asp700I GAANNNNTTC 3 cut(s) 606, 895, 1055
AspLEI GCGC 2 cut(s) 143, 145
AspS9I GGNCC 1 cut(s) 995
AsuHPI GGTGA 3 cut(s) 343, 484, 823
AvaI CYCGRG 1 cut(s) 184
BamHI GGATCC 1 cut(s) 92
BanII GRGCYC 1 cut(s) 63
BarI GAAGNNNNNNTAC 1 cut(s) 1046
BauI CACGAG 2 cut(s) 549, 641
Bbv12I GWGCWC 1 cut(s) 63
BbvI GCAGC 2 cut(s) 4, 387
BccI CCATC 3 cut(s) 155, 269, 1006
BceAI ACGGC 1 cut(s) 1054
BciT130I CCWGG 2 cut(s) 105, 909
BciVI GTATCC 1 cut(s) 15
BcoDI GTCTC 1 cut(s) 873
BfaI CTAG 1 cut(s) 785
BfrI CTTAAG 1 cut(s) 1040
BfuI GTATCC 1 cut(s) 15
BisI GCNGC 2 cut(s) 18, 376
BlsI GCNGC 2 cut(s) 19, 377
Bme1390I CCNGG 2 cut(s) 105, 909
BmeT110I CYCGRG 1 cut(s) 184
BmgT120I GGNCC 1 cut(s) 995
BmiI GGNNCC 3 cut(s) 94, 535, 1030
BmrFI CCNGG 2 cut(s) 105, 909
BmsI GCATC 4 cut(s) 128, 324, 710, 851
BsaBI GATNNNNATC 1 cut(s) 126
BsaJI CCNNGG 1 cut(s) 744
Bsc4I CCNNNNNNNGG 2 cut(s) 104, 908
Bse1I ACTGG 3 cut(s) 237, 350, 611
Bse3DI GCAATG 1 cut(s) 574
Bse8I GATNNNNATC 1 cut(s) 126
BseBI CCWGG 2 cut(s) 105, 909
BseDI CCNNGG 1 cut(s) 744
BseGI GGATG 3 cut(s) 406, 439, 842
BseJI GATNNNNATC 1 cut(s) 126
BseLI CCNNNNNNNGG 2 cut(s) 104, 908
BseMI GCAATG 1 cut(s) 574
BseMII CTCAG 2 cut(s) 368, 1022
BseNI ACTGG 3 cut(s) 237, 350, 611
BsePI GCGCGC 1 cut(s) 141
BseRI GAGGAG 2 cut(s) 71, 247
BseXI GCAGC 2 cut(s) 4, 387
BsgI GTGCAG 1 cut(s) 416
Bsh1236I CGCG 4 cut(s) 143, 517, 576, 873
BshFI GGCC 3 cut(s) 236, 300, 997
BsiHKAI GWGCWC 1 cut(s) 63
BsiHKCI CYCGRG 1 cut(s) 184
BslI CCNNNNNNNGG 2 cut(s) 104, 908
BsmAI GTCTC 1 cut(s) 873
BsmI GAATGC 2 cut(s) 758, 1018
BsnI GGCC 3 cut(s) 236, 300, 997
BsoBI CYCGRG 1 cut(s) 184
Bsp1286I GDGCHC 1 cut(s) 63
Bsp143I GATC 5 cut(s) 92, 526, 571, 685, 854
Bsp68I TCGCGA 2 cut(s) 576, 873
BspACI CCGC 3 cut(s) 8, 98, 515
BspANI GGCC 3 cut(s) 236, 300, 997
BspCNI CTCAG 2 cut(s) 367, 1021
BspFNI CGCG 4 cut(s) 143, 517, 576, 873
BspHI TCATGA 1 cut(s) 310
BspLI GGNNCC 3 cut(s) 94, 535, 1030
BspPI GGATC 5 cut(s) 87, 100, 534, 693, 862
BspTI CTTAAG 1 cut(s) 1040
BsrDI GCAATG 1 cut(s) 574
BsrI ACTGG 3 cut(s) 237, 350, 611
BssECI CCNNGG 1 cut(s) 744
BssHII GCGCGC 1 cut(s) 141
BssMI GATC 5 cut(s) 92, 526, 571, 685, 854
BssSI CACGAG 2 cut(s) 549, 641
Bst2BI CACGAG 2 cut(s) 549, 641
Bst2UI CCWGG 2 cut(s) 105, 909
Bst4CI ACNGT 1 cut(s) 45
Bst6I CTCTTC 1 cut(s) 853
BstAFI CTTAAG 1 cut(s) 1040
BstAPI GCANNNNNTGC 1 cut(s) 637
BstC8I GCNNGC 5 cut(s) 27, 143, 545, 565, 961
BstDEI CTNAG 4 cut(s) 73, 354, 428, 1008
BstF5I GGATG 3 cut(s) 406, 439, 842
BstFNI CGCG 4 cut(s) 143, 517, 576, 873
BstHHI GCGC 2 cut(s) 143, 145
BstKTI GATC 5 cut(s) 95, 529, 574, 688, 857
BstMAI GTCTC 1 cut(s) 873
BstMBI GATC 5 cut(s) 92, 526, 571, 685, 854
BstMWI GCNNNNNNNGC 3 cut(s) 26, 573, 637
BstNI CCWGG 2 cut(s) 105, 909
BstSCI CCNGG 2 cut(s) 103, 907
BstUI CGCG 4 cut(s) 143, 517, 576, 873
BstV1I GCAGC 2 cut(s) 4, 387
BstX2I RGATCY 3 cut(s) 92, 685, 854
BstYI RGATCY 3 cut(s) 92, 685, 854
BsuI GTATCC 1 cut(s) 15
BsuRI GGCC 3 cut(s) 236, 300, 997
BtsCI GGATG 3 cut(s) 406, 439, 842
BtsI GCAGTG 1 cut(s) 221
BtsIMutI CAGTG 2 cut(s) 50, 221
BtuMI TCGCGA 2 cut(s) 576, 873
Cac8I GCNNGC 5 cut(s) 27, 143, 545, 565, 961
CciI TCATGA 1 cut(s) 310
CfoI GCGC 2 cut(s) 143, 145
Cfr13I GGNCC 1 cut(s) 995
Csp6I GTAC 2 cut(s) 48, 172
CviAII CATG 2 cut(s) 311, 1066
CviQI GTAC 2 cut(s) 48, 172
DdeI CTNAG 4 cut(s) 73, 354, 428, 1008
DpnI GATC 5 cut(s) 94, 528, 573, 687, 856
DpnII GATC 5 cut(s) 92, 526, 571, 685, 854
Eam1104I CTCTTC 1 cut(s) 853
EarI CTCTTC 1 cut(s) 853
Ecl136II GAGCTC 1 cut(s) 61
Eco24I GRGCYC 1 cut(s) 63
Eco32I GATATC 1 cut(s) 34
Eco53kI GAGCTC 1 cut(s) 61
Eco57I CTGAAG 2 cut(s) 446, 834
Eco88I CYCGRG 1 cut(s) 184
EcoICRI GAGCTC 1 cut(s) 61
EcoRI GAATTC 2 cut(s) 287, 475
EcoRII CCWGG 2 cut(s) 103, 907
EcoRV GATATC 1 cut(s) 34
EcoT38I GRGCYC 1 cut(s) 63
FaeI CATG 2 cut(s) 314, 1069
FaiI YATR 4 cut(s) 267, 312, 638, 1067
FatI CATG 2 cut(s) 310, 1065
FauI CCCGC 1 cut(s) 105
Fnu4HI GCNGC 2 cut(s) 18, 376
FokI GGATG 3 cut(s) 413, 446, 829
FriOI GRGCYC 1 cut(s) 63
Fsp4HI GCNGC 2 cut(s) 18, 376
FspBI CTAG 1 cut(s) 785
GlaI GCGC 2 cut(s) 142, 144
GluI GCNGC 2 cut(s) 18, 376
HaeIII GGCC 3 cut(s) 236, 300, 997
HhaI GCGC 2 cut(s) 143, 145
Hin1II CATG 2 cut(s) 314, 1069
Hin6I GCGC 2 cut(s) 141, 143
HinP1I GCGC 2 cut(s) 141, 143
HindIII AAGCTT 2 cut(s) 458, 1037
HinfI GANTC 5 cut(s) 115, 420, 761, 885, 904
HphI GGTGA 3 cut(s) 343, 484, 823
Hpy166II GTNNAC 1 cut(s) 48
Hpy188I TCNGA 8 cut(s) 357, 370, 481, 748, 890, 903, 979, 1011
Hpy188III TCNNGA 9 cut(s) 119, 311, 464, 575, 617, 660, 689, 852, 872
Hpy8I GTNNAC 1 cut(s) 48
Hpy99I CGWCG 1 cut(s) 370
HpyAV CCTTC 1 cut(s) 986
HpyCH4III ACNGT 1 cut(s) 45
HpyCH4V TGCA 7 cut(s) 337, 397, 417, 563, 631, 640, 842
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 573, 637
HpyF3I CTNAG 4 cut(s) 73, 354, 428, 1008
Hsp92II CATG 2 cut(s) 314, 1069
HspAI GCGC 2 cut(s) 141, 143
Kzo9I GATC 5 cut(s) 92, 526, 571, 685, 854
LmnI GCTCC 1 cut(s) 58
Lsp1109I GCAGC 2 cut(s) 4, 387
LweI GCATC 4 cut(s) 128, 324, 710, 851
MaeI CTAG 1 cut(s) 785
MaeIII GTNAC 3 cut(s) 595, 811, 934
MalI GATC 5 cut(s) 94, 528, 573, 687, 856
MboI GATC 5 cut(s) 92, 526, 571, 685, 854
MboII GAAGA 5 cut(s) 370, 397, 791, 840, 1060
MfeI CAATTG 1 cut(s) 325
MflI RGATCY 3 cut(s) 92, 685, 854
MhlI GDGCHC 1 cut(s) 63
MlyI GAGTC 1 cut(s) 429
MmeI TCCRAC 3 cut(s) 251, 348, 756
MroXI GAANNNNTTC 3 cut(s) 606, 895, 1055
MseI TTAA 1 cut(s) 1041
MslI CAYNNNNRTG 1 cut(s) 645
MspA1I CMGCKG 1 cut(s) 17
MspCI CTTAAG 1 cut(s) 1040
MspR9I CCNGG 2 cut(s) 105, 909
MunI CAATTG 1 cut(s) 325
Mva1269I GAATGC 2 cut(s) 758, 1018
MvaI CCWGG 2 cut(s) 105, 909
MvnI CGCG 4 cut(s) 143, 517, 576, 873
MwoI GCNNNNNNNGC 3 cut(s) 26, 573, 637
NdeII GATC 5 cut(s) 92, 526, 571, 685, 854
NlaIII CATG 2 cut(s) 314, 1069
NlaIV GGNNCC 3 cut(s) 94, 535, 1030
NmuCI GTSAC 2 cut(s) 811, 934
NruI TCGCGA 2 cut(s) 576, 873
PaeR7I CTCGAG 1 cut(s) 184
PagI TCATGA 1 cut(s) 310
PauI GCGCGC 1 cut(s) 141
PcsI WCGNNNNNNNCGW 1 cut(s) 756
PctI GAATGC 2 cut(s) 758, 1018
PdmI GAANNNNTTC 3 cut(s) 606, 895, 1055
PfeI GAWTC 4 cut(s) 115, 761, 885, 904
PflFI GACNNNGTC 1 cut(s) 739
PfoI TCCNGGA 1 cut(s) 103
PkrI GCNGC 2 cut(s) 19, 377
PleI GAGTC 1 cut(s) 428
PpsI GAGTC 1 cut(s) 428
Psp124BI GAGCTC 1 cut(s) 63
Psp6I CCWGG 2 cut(s) 103, 907
PspGI CCWGG 2 cut(s) 103, 907
PspN4I GGNNCC 3 cut(s) 94, 535, 1030
PspPI GGNCC 1 cut(s) 995
PspXI VCTCGAGB 1 cut(s) 184
PsuI RGATCY 3 cut(s) 92, 685, 854
PsyI GACNNNGTC 1 cut(s) 739
PteI GCGCGC 1 cut(s) 141
PvuII CAGCTG 1 cut(s) 17
RruI TCGCGA 2 cut(s) 576, 873
RsaI GTAC 2 cut(s) 49, 173
RsaNI GTAC 2 cut(s) 48, 172
RseI CAYNNNNRTG 1 cut(s) 645
SacI GAGCTC 1 cut(s) 63
SaqAI TTAA 1 cut(s) 1041
SatI GCNGC 2 cut(s) 18, 376
Sau3AI GATC 5 cut(s) 92, 526, 571, 685, 854
Sau96I GGNCC 1 cut(s) 995
SchI GAGTC 1 cut(s) 429
ScrFI CCNGG 2 cut(s) 105, 909
SduI GDGCHC 1 cut(s) 63
SetI ASST 7 cut(s) 19, 41, 63, 280, 462, 657, 1041
SfaNI GCATC 4 cut(s) 128, 324, 710, 851
Sfr274I CTCGAG 1 cut(s) 184
SlaI CTCGAG 1 cut(s) 184
SmiMI CAYNNNNRTG 1 cut(s) 645
SmlI CTYRAG 2 cut(s) 184, 1040
SmoI CTYRAG 2 cut(s) 184, 1040
SsiI CCGC 3 cut(s) 8, 98, 515
SspI AATATT 2 cut(s) 208, 246
SspMI CTAG 1 cut(s) 785
SstI GAGCTC 1 cut(s) 63
StyD4I CCNGG 2 cut(s) 103, 907
TaaI ACNGT 1 cut(s) 45
TaqI TCGA 9 cut(s) 118, 149, 185, 295, 616, 659, 725, 759, 861
TfiI GAWTC 4 cut(s) 115, 761, 885, 904
Tru1I TTAA 1 cut(s) 1041
Tru9I TTAA 1 cut(s) 1041
TscAI CASTG 2 cut(s) 50, 221
TseFI GTSAC 2 cut(s) 811, 934
TseI GCWGC 2 cut(s) 17, 375
Tsp45I GTSAC 2 cut(s) 811, 934
TspDTI ATGAA 6 cut(s) 166, 327, 453, 699, 1005, 1054
TspGWI ACGGA 1 cut(s) 105
TspRI CASTG 2 cut(s) 50, 221
Tth111I GACNNNGTC 1 cut(s) 739
Vha464I CTTAAG 1 cut(s) 1040
XapI RAATTY 4 cut(s) 151, 287, 475, 1003
XhoI CTCGAG 1 cut(s) 184
XmnI GAANNNNTTC 3 cut(s) 606, 895, 1055
XspI CTAG 1 cut(s) 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.