RchiOBHm_Chr6g0300521
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0005177
Physical Location & Seq
Reverse (-)
61355226 .. 61358406
3181 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26989

Sequence Viewer

Length: 1140 bp
ATGCATCCACAAGCTGCACTCCTGCCTGCTGATATTGGTCTTCACTGTACCGATGAGGAGCTCCGTATGTTTACGTTTTTACAGAAACTTATAAATGGATCACCACCTCCAACAAATGTGATCAATGGTGTAAATCCTTACCAATACTTGCCCTCAAATTTACCACCTGGTAGTCTTTGGTACTTGATTCGCTCTAATGAGAGCAAAGATACAAATTTTGGATACTGGAGGCCCAAAGAGGAGGCATGTCAAATATTCTCAGACTCTACCATCACTGGTTGGAGAACTACCCTTGAATTCTATGAAGGCCGAGCACCTCATGAACAAAAAACCAATTGGGTAATGCAAGAATACTGGATAACTTACGAGAAAGTTTCCGAAAACAGCAAGACAAAGGAAGCCAGTTTGCTTTGCAGAGTCCAATCTATTGGTGCTGAACCGAAATCAAACTGCAAAAACCTCGAGAAAAAGGTTACTTCTGATGTTGCTAATCACTTCACACATTTAGTTGTTCCACTAGTGGAGAAACGTACTGGACACGCTTCCACAAGCAAGCCTCAGGTGATCAAGGATAATGAAACAGGAACGTTAACTGTGACAGAAAGGCTTCCAGATCATCAAATAGAAATCATGCCCGAGACAGAGATAGATTTTCCTTTCGGAGATGACTACATAGAATTGCTGGATCTTGATAGACCAGCATCATTTTCTTCTAGTTCAGATTCAAGTTGCTTGACAATATCATCAGACGAATGCTTCGATTCCCTGGCTTTGTTGGAAGAACTAGAACCCAAAACTAGTCCGGACCGTGCAAATAAGAATGCAGGCTGCAAATTCAGTATTTCAGTATGTCAAAGACCAGATGAGTTGGTTATGTATGCAGCTGCCGCTTCCTCAGGATCTTTGAACAAATCTCCAAGTGAAGAAAGGATCAAAAGTCATTCTTCAATTCCCAGTTCAACAGTCTGCGTTAAGAGTAAAGAAAAAACTACAGTGAAACTGAGCAGAAATCAAAAACCAGACCAAAGGAATGAAGGTTTCTCGAATTCTCATACGACAACACCTCCAGCAGGAAAGAAGGCTACTGTTGTCAAAACTAAGAAGCTTAAGAAAAAGTATCTTTGCTGCTTGCCATGTTAG

Protein Analysis

379

Amino Acids

42.36

Weight (kDa)

6.53

Isoelectric Point (pI)

48.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 12 - 118 4.4e-15 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 92
AccIII TCCGGA 1 cut(s) 802
AciI CCGC 1 cut(s) 888
AclI AACGTT 1 cut(s) 587
AclWI GGATC 4 cut(s) 106, 693, 907, 938
AcsI RAATTY 5 cut(s) 157, 214, 296, 833, 1045
AfaI GTAC 3 cut(s) 49, 182, 532
AfiI CCNNNNNNNGG 1 cut(s) 1070
AflII CTTAAG 1 cut(s) 1106
AgsI TTSAA 5 cut(s) 296, 726, 907, 948, 960
AhlI ACTAGT 2 cut(s) 517, 797
AjnI CCWGG 2 cut(s) 166, 765
AluBI AGCT 4 cut(s) 14, 61, 884, 1105
AluI AGCT 4 cut(s) 14, 61, 884, 1105
Alw21I GWGCWC 2 cut(s) 63, 316
Alw26I GTCTC 1 cut(s) 632
AlwI GGATC 4 cut(s) 106, 693, 907, 938
Ama87I CYCGRG 2 cut(s) 461, 635
Aor13HI TCCGGA 1 cut(s) 802
AoxI GGCC 2 cut(s) 230, 307
ApeKI GCWGC 5 cut(s) 14, 828, 881, 884, 1125
ApoI RAATTY 5 cut(s) 157, 214, 296, 833, 1045
ArsI GACNNNNNNTTYG 2 cut(s) 740, 772
Asp700I GAANNNNTTC 1 cut(s) 606
AspS9I GGNCC 2 cut(s) 231, 805
AsuHPI GGTGA 2 cut(s) 93, 574
AvaI CYCGRG 2 cut(s) 461, 635
AvaII GGWCC 1 cut(s) 805
AxyI CCTNAGG 2 cut(s) 558, 895
BanII GRGCYC 1 cut(s) 63
BbsI GAAGAC 1 cut(s) 32
Bbv12I GWGCWC 2 cut(s) 63, 316
BbvI GCAGC 4 cut(s) 815, 871, 893, 1112
BccI CCATC 1 cut(s) 278
BcgI CGANNNNNNTGC 2 cut(s) 442, 476
BciT130I CCWGG 2 cut(s) 168, 767
BciVI GTATCC 1 cut(s) 215
BclI TGATCA 2 cut(s) 120, 564
BcoDI GTCTC 1 cut(s) 632
BcuI ACTAGT 2 cut(s) 517, 797
BfaI CTAG 4 cut(s) 518, 714, 785, 798
BfmI CTRYAG 1 cut(s) 990
BfrI CTTAAG 1 cut(s) 1106
BfuI GTATCC 1 cut(s) 215
BisI GCNGC 6 cut(s) 15, 829, 882, 885, 888, 1126
BlsI GCNGC 6 cut(s) 16, 830, 883, 886, 889, 1127
Bme1390I CCNGG 2 cut(s) 168, 767
Bme18I GGWCC 1 cut(s) 805
BmeT110I CYCGRG 2 cut(s) 461, 635
BmgT120I GGNCC 2 cut(s) 231, 805
BmrFI CCNGG 2 cut(s) 168, 767
BmrI ACTGGG 1 cut(s) 948
BmsI GCATC 2 cut(s) 13, 710
BmuI ACTGGG 1 cut(s) 948
BpiI GAAGAC 1 cut(s) 32
BpmI CTGGAG 2 cut(s) 247, 1050
BsaJI CCNNGG 1 cut(s) 765
BsaWI WCCGGW 1 cut(s) 802
BsaXI ACNNNNNCTCC 2 cut(s) 1048, 1078
Bsc4I CCNNNNNNNGG 1 cut(s) 1070
Bse1I ACTGG 6 cut(s) 230, 280, 359, 402, 538, 954
Bse21I CCTNAGG 2 cut(s) 558, 895
BseAI TCCGGA 1 cut(s) 802
BseBI CCWGG 2 cut(s) 168, 767
BseDI CCNNGG 1 cut(s) 765
BseGI GGATG 1 cut(s) 4
BseLI CCNNNNNNNGG 1 cut(s) 1070
BseMII CTCAG 4 cut(s) 273, 572, 909, 992
BseNI ACTGG 6 cut(s) 230, 280, 359, 402, 538, 954
BseRI GAGGAG 2 cut(s) 71, 254
BseXI GCAGC 4 cut(s) 815, 871, 893, 1112
BshFI GGCC 2 cut(s) 232, 309
BsiHKAI GWGCWC 2 cut(s) 63, 316
BsiHKCI CYCGRG 2 cut(s) 461, 635
BsiSI CCGG 1 cut(s) 803
BslI CCNNNNNNNGG 1 cut(s) 1070
BsmAI GTCTC 1 cut(s) 632
BsmI GAATGC 2 cut(s) 758, 826
BsnI GGCC 2 cut(s) 232, 309
BsoBI CYCGRG 2 cut(s) 461, 635
Bsp1286I GDGCHC 2 cut(s) 63, 316
Bsp13I TCCGGA 1 cut(s) 802
Bsp143I GATC 7 cut(s) 98, 120, 564, 613, 685, 899, 930
BspACI CCGC 1 cut(s) 888
BspANI GGCC 2 cut(s) 232, 309
BspCNI CTCAG 4 cut(s) 272, 571, 908, 993
BspEI TCCGGA 1 cut(s) 802
BspHI TCATGA 1 cut(s) 319
BspPI GGATC 4 cut(s) 106, 693, 907, 938
BspTI CTTAAG 1 cut(s) 1106
BsrI ACTGG 6 cut(s) 230, 280, 359, 402, 538, 954
BssECI CCNNGG 1 cut(s) 765
BssMI GATC 7 cut(s) 98, 120, 564, 613, 685, 899, 930
Bst2UI CCWGG 2 cut(s) 168, 767
Bst4CI ACNGT 6 cut(s) 47, 595, 809, 964, 994, 1087
BstAFI CTTAAG 1 cut(s) 1106
BstC8I GCNNGC 4 cut(s) 27, 554, 826, 1130
BstDEI CTNAG 5 cut(s) 259, 558, 895, 1001, 1098
BstENI CCTNNNNNAGG 1 cut(s) 1068
BstF5I GGATG 1 cut(s) 4
BstKTI GATC 7 cut(s) 101, 123, 567, 616, 688, 902, 933
BstMAI GTCTC 1 cut(s) 632
BstMBI GATC 7 cut(s) 98, 120, 564, 613, 685, 899, 930
BstMWI GCNNNNNNNGC 1 cut(s) 887
BstNI CCWGG 2 cut(s) 168, 767
BstNSI RCATGY 1 cut(s) 249
BstSCI CCNGG 2 cut(s) 166, 765
BstSFI CTRYAG 1 cut(s) 990
BstV1I GCAGC 4 cut(s) 815, 871, 893, 1112
BstV2I GAAGAC 1 cut(s) 32
BstX2I RGATCY 2 cut(s) 685, 899
BstXI CCANNNNNNTGG 1 cut(s) 428
BstYI RGATCY 2 cut(s) 685, 899
Bsu36I CCTNAGG 2 cut(s) 558, 895
BsuI GTATCC 1 cut(s) 215
BsuRI GGCC 2 cut(s) 232, 309
BtsCI GGATG 1 cut(s) 4
BtsIMutI CAGTG 3 cut(s) 43, 273, 999
Cac8I GCNNGC 4 cut(s) 27, 554, 826, 1130
CciI TCATGA 1 cut(s) 319
Cfr13I GGNCC 2 cut(s) 231, 805
CpoI CGGWCCG 1 cut(s) 805
CsiI ACCWGGT 1 cut(s) 166
Csp6I GTAC 3 cut(s) 48, 181, 531
CspI CGGWCCG 1 cut(s) 805
CviAII CATG 4 cut(s) 246, 320, 631, 1134
CviQI GTAC 3 cut(s) 48, 181, 531
DdeI CTNAG 5 cut(s) 259, 558, 895, 1001, 1098
DpnI GATC 7 cut(s) 100, 122, 566, 615, 687, 901, 932
DpnII GATC 7 cut(s) 98, 120, 564, 613, 685, 899, 930
Ecl136II GAGCTC 1 cut(s) 61
Eco24I GRGCYC 1 cut(s) 63
Eco47I GGWCC 1 cut(s) 805
Eco53kI GAGCTC 1 cut(s) 61
Eco81I CCTNAGG 2 cut(s) 558, 895
Eco88I CYCGRG 2 cut(s) 461, 635
EcoICRI GAGCTC 1 cut(s) 61
EcoNI CCTNNNNNAGG 1 cut(s) 1068
EcoRI GAATTC 2 cut(s) 296, 1045
EcoRII CCWGG 2 cut(s) 166, 765
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 63
FaeI CATG 4 cut(s) 249, 323, 634, 1137
FalI AAGNNNNNCTT 2 cut(s) 928, 960
FatI CATG 4 cut(s) 245, 319, 630, 1133
FbaI TGATCA 2 cut(s) 120, 564
Fnu4HI GCNGC 6 cut(s) 15, 829, 882, 885, 888, 1126
FriOI GRGCYC 1 cut(s) 63
Fsp4HI GCNGC 6 cut(s) 15, 829, 882, 885, 888, 1126
FspBI CTAG 4 cut(s) 518, 714, 785, 798
GluI GCNGC 6 cut(s) 15, 829, 882, 885, 888, 1126
GsuI CTGGAG 2 cut(s) 247, 1050
HaeIII GGCC 2 cut(s) 232, 309
HapII CCGG 1 cut(s) 803
Hin1II CATG 4 cut(s) 249, 323, 634, 1137
HincII GTYRAC 1 cut(s) 591
HindII GTYRAC 1 cut(s) 591
HindIII AAGCTT 1 cut(s) 1103
HinfI GANTC 5 cut(s) 187, 263, 417, 722, 761
HpaI GTTAAC 1 cut(s) 591
HpaII CCGG 1 cut(s) 803
HphI GGTGA 2 cut(s) 93, 574
Hpy166II GTNNAC 2 cut(s) 72, 591
Hpy188I TCNGA 6 cut(s) 262, 379, 481, 662, 721, 748
Hpy188III TCNNGA 7 cut(s) 320, 463, 611, 689, 803, 897, 1042
Hpy8I GTNNAC 2 cut(s) 72, 591
HpyAV CCTTC 3 cut(s) 299, 1028, 1072
HpyCH4III ACNGT 6 cut(s) 47, 595, 809, 964, 994, 1087
HpyCH4IV ACGT 3 cut(s) 74, 529, 587
HpyCH4V TGCA 9 cut(s) 4, 17, 346, 414, 453, 812, 824, 831, 881
HpyF10VI GCNNNNNNNGC 1 cut(s) 887
HpyF3I CTNAG 5 cut(s) 259, 558, 895, 1001, 1098
HpySE526I ACGT 3 cut(s) 74, 529, 587
Hsp92II CATG 4 cut(s) 249, 323, 634, 1137
Kpn2I TCCGGA 1 cut(s) 802
Ksp22I TGATCA 2 cut(s) 120, 564
KspAI GTTAAC 1 cut(s) 591
Kzo9I GATC 7 cut(s) 98, 120, 564, 613, 685, 899, 930
LmnI GCTCC 2 cut(s) 58, 66
Lsp1109I GCAGC 4 cut(s) 815, 871, 893, 1112
LweI GCATC 2 cut(s) 13, 710
MabI ACCWGGT 1 cut(s) 166
MaeI CTAG 4 cut(s) 518, 714, 785, 798
MaeII ACGT 3 cut(s) 74, 529, 587
MaeIII GTNAC 2 cut(s) 472, 595
MalI GATC 7 cut(s) 100, 122, 566, 615, 687, 901, 932
MboI GATC 7 cut(s) 98, 120, 564, 613, 685, 899, 930
MboII GAAGA 5 cut(s) 32, 702, 791, 935, 936
MfeI CAATTG 1 cut(s) 334
MflI RGATCY 2 cut(s) 685, 899
MhlI GDGCHC 2 cut(s) 63, 316
MluCI AATT 8 cut(s) 157, 214, 296, 334, 677, 833, 948, 1045
MlyI GAGTC 2 cut(s) 257, 426
MmeI TCCRAC 3 cut(s) 134, 260, 756
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 802
MroXI GAANNNNTTC 1 cut(s) 606
MseI TTAA 3 cut(s) 590, 972, 1107
MspA1I CMGCKG 1 cut(s) 884
MspCI CTTAAG 1 cut(s) 1106
MspI CCGG 1 cut(s) 803
MspR9I CCNGG 2 cut(s) 168, 767
MunI CAATTG 1 cut(s) 334
Mva1269I GAATGC 2 cut(s) 758, 826
MvaI CCWGG 2 cut(s) 168, 767
MwoI GCNNNNNNNGC 1 cut(s) 887
NdeII GATC 7 cut(s) 98, 120, 564, 613, 685, 899, 930
NlaIII CATG 4 cut(s) 249, 323, 634, 1137
NmeAIII GCCGAG 1 cut(s) 335
NmuCI GTSAC 1 cut(s) 595
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 249
PaeR7I CTCGAG 1 cut(s) 461
PagI TCATGA 1 cut(s) 319
PcsI WCGNNNNNNNCGW 1 cut(s) 756
PctI GAATGC 2 cut(s) 758, 826
PdmI GAANNNNTTC 1 cut(s) 606
PfeI GAWTC 3 cut(s) 187, 722, 761
PkrI GCNGC 6 cut(s) 16, 830, 883, 886, 889, 1127
PleI GAGTC 2 cut(s) 257, 425
PpsI GAGTC 2 cut(s) 257, 425
PsiI TTATAA 1 cut(s) 92
Psp124BI GAGCTC 1 cut(s) 63
Psp1406I AACGTT 1 cut(s) 587
Psp6I CCWGG 2 cut(s) 166, 765
PspGI CCWGG 2 cut(s) 166, 765
PspPI GGNCC 2 cut(s) 231, 805
PsuI RGATCY 2 cut(s) 685, 899
PvuII CAGCTG 1 cut(s) 884
RsaI GTAC 3 cut(s) 49, 182, 532
RsaNI GTAC 3 cut(s) 48, 181, 531
Rsr2I CGGWCCG 1 cut(s) 805
RsrII CGGWCCG 1 cut(s) 805
SacI GAGCTC 1 cut(s) 63
SaqAI TTAA 3 cut(s) 590, 972, 1107
SatI GCNGC 6 cut(s) 15, 829, 882, 885, 888, 1126
Sau3AI GATC 7 cut(s) 98, 120, 564, 613, 685, 899, 930
Sau96I GGNCC 2 cut(s) 231, 805
SchI GAGTC 2 cut(s) 257, 426
ScrFI CCNGG 2 cut(s) 168, 767
SduI GDGCHC 2 cut(s) 63, 316
SexAI ACCWGGT 1 cut(s) 166
SfaNI GCATC 2 cut(s) 13, 710
SfcI CTRYAG 1 cut(s) 990
Sfr274I CTCGAG 1 cut(s) 461
SinI GGWCC 1 cut(s) 805
SlaI CTCGAG 1 cut(s) 461
SmlI CTYRAG 2 cut(s) 461, 1106
SmoI CTYRAG 2 cut(s) 461, 1106
SpeI ACTAGT 2 cut(s) 517, 797
Sse9I AATT 8 cut(s) 157, 214, 296, 334, 677, 833, 948, 1045
SsiI CCGC 1 cut(s) 888
SspI AATATT 1 cut(s) 255
SspMI CTAG 4 cut(s) 518, 714, 785, 798
SstI GAGCTC 1 cut(s) 63
StyD4I CCNGG 2 cut(s) 166, 765
TaaI ACNGT 6 cut(s) 47, 595, 809, 964, 994, 1087
TaiI ACGT 3 cut(s) 77, 532, 590
TaqI TCGA 3 cut(s) 462, 759, 1043
TasI AATT 8 cut(s) 157, 214, 296, 334, 677, 833, 948, 1045
TauI GCSGC 1 cut(s) 890
TfiI GAWTC 3 cut(s) 187, 722, 761
Tru1I TTAA 3 cut(s) 590, 972, 1107
Tru9I TTAA 3 cut(s) 590, 972, 1107
TscAI CASTG 3 cut(s) 50, 280, 999
TseFI GTSAC 1 cut(s) 595
TseI GCWGC 5 cut(s) 14, 828, 881, 884, 1125
Tsp45I GTSAC 1 cut(s) 595
TspDTI ATGAA 4 cut(s) 318, 336, 591, 1047
TspGWI ACGGA 1 cut(s) 53
TspRI CASTG 3 cut(s) 50, 280, 999
Vha464I CTTAAG 1 cut(s) 1106
VpaK11BI GGWCC 1 cut(s) 805
XagI CCTNNNNNAGG 1 cut(s) 1068
XapI RAATTY 5 cut(s) 157, 214, 296, 833, 1045
XceI RCATGY 1 cut(s) 249
XhoI CTCGAG 1 cut(s) 461
XmnI GAANNNNTTC 1 cut(s) 606
XspI CTAG 4 cut(s) 518, 714, 785, 798
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.