Rorug05G0015600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0005177
Physical Location & Seq
Reverse (-)
1052048 .. 1052578
531 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0015600.1

Sequence Viewer

Length: 531 bp
ATGAGAGCCTCCAGGCAGCACAAGAAGCAAGCGCAGCTGCAACGTAGAGCTCCGATTCGGGCATTGTGTTGCAGTAGCTGCAGGCTCAGCGTGTCTTCGTCTTCCGAGGAAGTAGAGAGCTCCAGCACTTCTGATCGTAACAGGTACCCGTCGATATCAAGCTTAGCGCATGCGATGGTTCAAGAGAGACTAGACCAAATGATTAGAGAGAGGCAGGAGGAGGATGCCGCGAGAGTAGTGCATGTGGATCGTCGGAGAAGAAAGAACAAAGTAGTATATGATGATCGAGCACAAGCAACAGGGACTAAATTTGTCGTAATGGTTGCGATGGAAAAGTGTTCCTATGATCCGAGAGAAGATTTTAGGGAGTCCATCGCGGAGATGATAATGGCAAAGCGGATCGAAGAGCCAAAGGATCTTCGTAGCCTCTTGAATTACTATGTTTCGATGAATTCGGAGGAGTATCACGGGATTATACTCGAGGTGTTTCATGAAGTTTGCTCTGATTTGTTCCTATGTAAATGCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

176

Amino Acids

20.54

Weight (kDa)

8.48

Isoelectric Point (pI)

70.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ovate PF04844 114 - 171 7.1e-22 Transcriptional repressor, ovate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 144
AccB1I GGYRCC 1 cut(s) 144
AccII CGCG 2 cut(s) 230, 377
AciI CCGC 3 cut(s) 228, 377, 397
AclWI GGATC 4 cut(s) 255, 341, 407, 423
AcsI RAATTY 2 cut(s) 308, 451
AfaI GTAC 1 cut(s) 146
AgsI TTSAA 2 cut(s) 182, 433
AjnI CCWGG 1 cut(s) 11
AluBI AGCT 5 cut(s) 37, 50, 78, 120, 162
AluI AGCT 5 cut(s) 37, 50, 78, 120, 162
Alw21I GWGCWC 3 cut(s) 52, 122, 292
Alw26I GTCTC 1 cut(s) 181
AlwI GGATC 4 cut(s) 255, 341, 407, 423
AlwNI CAGNNNCTG 1 cut(s) 78
Ama87I CYCGRG 1 cut(s) 479
ApeKI GCWGC 4 cut(s) 16, 34, 37, 78
ApoI RAATTY 2 cut(s) 308, 451
Asp718I GGTACC 1 cut(s) 144
AspLEI GCGC 2 cut(s) 34, 169
AvaI CYCGRG 1 cut(s) 479
BanI GGYRCC 1 cut(s) 144
BanII GRGCYC 2 cut(s) 52, 122
BbsI GAAGAC 2 cut(s) 87, 93
Bbv12I GWGCWC 3 cut(s) 52, 122, 292
BbvI GCAGC 4 cut(s) 24, 28, 46, 65
BccI CCATC 3 cut(s) 169, 322, 380
BcgI CGANNNNNNTGC 4 cut(s) 220, 230, 254, 264
BciT130I CCWGG 1 cut(s) 13
BcoDI GTCTC 1 cut(s) 181
BfaI CTAG 1 cut(s) 191
BfmI CTRYAG 1 cut(s) 79
BisI GCNGC 5 cut(s) 17, 35, 38, 79, 228
BlpI GCTNAGC 2 cut(s) 86, 163
BlsI GCNGC 5 cut(s) 18, 36, 39, 80, 229
Bme1390I CCNGG 1 cut(s) 13
BmeT110I CYCGRG 1 cut(s) 479
BmiI GGNNCC 1 cut(s) 146
BmrFI CCNGG 1 cut(s) 13
BmsI GCATC 1 cut(s) 214
BpiI GAAGAC 2 cut(s) 87, 93
BpmI CTGGAG 1 cut(s) 106
Bpu1102I GCTNAGC 2 cut(s) 86, 163
BsaJI CCNNGG 1 cut(s) 105
BseBI CCWGG 1 cut(s) 13
BseDI CCNNGG 1 cut(s) 105
BseGI GGATG 1 cut(s) 229
BseMII CTCAG 1 cut(s) 100
BseRI GAGGAG 2 cut(s) 233, 473
BseXI GCAGC 4 cut(s) 24, 28, 46, 65
Bsh1236I CGCG 2 cut(s) 230, 377
BshNI GGYRCC 1 cut(s) 144
BsiHKAI GWGCWC 3 cut(s) 52, 122, 292
BsiHKCI CYCGRG 1 cut(s) 479
BslFI GGGAC 1 cut(s) 316
BsmAI GTCTC 1 cut(s) 181
BsmFI GGGAC 1 cut(s) 316
BsoBI CYCGRG 1 cut(s) 479
Bsp1286I GDGCHC 3 cut(s) 52, 122, 292
Bsp143I GATC 6 cut(s) 133, 247, 283, 346, 399, 415
Bsp1720I GCTNAGC 2 cut(s) 86, 163
BspACI CCGC 3 cut(s) 228, 377, 397
BspCNI CTCAG 1 cut(s) 99
BspFNI CGCG 2 cut(s) 230, 377
BspHI TCATGA 1 cut(s) 490
BspLI GGNNCC 1 cut(s) 146
BspMAI CTGCAG 1 cut(s) 83
BspPI GGATC 4 cut(s) 255, 341, 407, 423
BspQI GCTCTTC 1 cut(s) 399
BspT107I GGYRCC 1 cut(s) 144
BssECI CCNNGG 1 cut(s) 105
BssMI GATC 6 cut(s) 133, 247, 283, 346, 399, 415
Bst2UI CCWGG 1 cut(s) 13
Bst6I CTCTTC 1 cut(s) 399
BstAPI GCANNNNNTGC 1 cut(s) 78
BstC8I GCNNGC 3 cut(s) 30, 83, 171
BstDEI CTNAG 2 cut(s) 86, 163
BstF5I GGATG 1 cut(s) 229
BstFNI CGCG 2 cut(s) 230, 377
BstHHI GCGC 2 cut(s) 34, 169
BstKTI GATC 6 cut(s) 136, 250, 286, 349, 402, 418
BstMAI GTCTC 1 cut(s) 181
BstMBI GATC 6 cut(s) 133, 247, 283, 346, 399, 415
BstMWI GCNNNNNNNGC 4 cut(s) 25, 34, 78, 87
BstNI CCWGG 1 cut(s) 13
BstNSI RCATGY 2 cut(s) 173, 245
BstSCI CCNGG 1 cut(s) 11
BstSFI CTRYAG 1 cut(s) 79
BstUI CGCG 2 cut(s) 230, 377
BstV1I GCAGC 4 cut(s) 24, 28, 46, 65
BstV2I GAAGAC 2 cut(s) 87, 93
BstX2I RGATCY 1 cut(s) 415
BstYI RGATCY 1 cut(s) 415
BtgZI GCGATG 3 cut(s) 188, 341, 358
BtsCI GGATG 1 cut(s) 229
Cac8I GCNNGC 3 cut(s) 30, 83, 171
CaiI CAGNNNCTG 1 cut(s) 78
CciI TCATGA 1 cut(s) 490
CfoI GCGC 2 cut(s) 34, 169
Csp6I GTAC 1 cut(s) 145
CviAII CATG 3 cut(s) 170, 242, 491
CviJI RGCY 9 cut(s) 8, 37, 50, 78, 85, 120, 162, 409, 426
CviKI_1 RGCY 9 cut(s) 8, 37, 50, 78, 85, 120, 162, 409, 426
CviQI GTAC 1 cut(s) 145
DdeI CTNAG 2 cut(s) 86, 163
DpnI GATC 6 cut(s) 135, 249, 285, 348, 401, 417
DpnII GATC 6 cut(s) 133, 247, 283, 346, 399, 415
Eam1104I CTCTTC 1 cut(s) 399
EarI CTCTTC 1 cut(s) 399
Ecl136II GAGCTC 2 cut(s) 50, 120
Eco24I GRGCYC 2 cut(s) 52, 122
Eco32I GATATC 1 cut(s) 156
Eco53kI GAGCTC 2 cut(s) 50, 120
Eco88I CYCGRG 1 cut(s) 479
EcoICRI GAGCTC 2 cut(s) 50, 120
EcoRI GAATTC 1 cut(s) 451
EcoRII CCWGG 1 cut(s) 11
EcoRV GATATC 1 cut(s) 156
EcoT38I GRGCYC 2 cut(s) 52, 122
FaeI CATG 3 cut(s) 173, 245, 494
FaiI YATR 9 cut(s) 171, 243, 277, 279, 345, 441, 476, 492, 517
FaqI GGGAC 1 cut(s) 316
FatI CATG 3 cut(s) 169, 241, 490
Fnu4HI GCNGC 5 cut(s) 17, 35, 38, 79, 228
FokI GGATG 1 cut(s) 236
FriOI GRGCYC 2 cut(s) 52, 122
Fsp4HI GCNGC 5 cut(s) 17, 35, 38, 79, 228
FspBI CTAG 1 cut(s) 191
GlaI GCGC 2 cut(s) 33, 168
GluI GCNGC 5 cut(s) 17, 35, 38, 79, 228
GsuI CTGGAG 1 cut(s) 106
HhaI GCGC 2 cut(s) 34, 169
Hin1II CATG 3 cut(s) 173, 245, 494
Hin6I GCGC 2 cut(s) 32, 167
HinP1I GCGC 2 cut(s) 32, 167
HindIII AAGCTT 1 cut(s) 160
HinfI GANTC 2 cut(s) 55, 368
Hpy188I TCNGA 7 cut(s) 54, 106, 133, 255, 351, 457, 505
Hpy188III TCNNGA 3 cut(s) 182, 430, 491
Hpy99I CGWCG 2 cut(s) 154, 255
HpyCH4IV ACGT 1 cut(s) 43
HpyCH4V TGCA 4 cut(s) 40, 72, 81, 241
HpyF10VI GCNNNNNNNGC 4 cut(s) 25, 34, 78, 87
HpyF3I CTNAG 2 cut(s) 86, 163
HpySE526I ACGT 1 cut(s) 43
Hsp92II CATG 3 cut(s) 173, 245, 494
HspAI GCGC 2 cut(s) 32, 167
KpnI GGTACC 1 cut(s) 148
Kzo9I GATC 6 cut(s) 133, 247, 283, 346, 399, 415
LguI GCTCTTC 1 cut(s) 399
LmnI GCTCC 2 cut(s) 55, 125
LpnPI CCDG 6 cut(s) 25, 67, 127, 136, 200, 285
Lsp1109I GCAGC 4 cut(s) 24, 28, 46, 65
LweI GCATC 1 cut(s) 214
MaeI CTAG 1 cut(s) 191
MaeII ACGT 1 cut(s) 43
MaeIII GTNAC 1 cut(s) 137
MalI GATC 6 cut(s) 135, 249, 285, 348, 401, 417
MboI GATC 6 cut(s) 133, 247, 283, 346, 399, 415
MboII GAAGA 6 cut(s) 87, 93, 270, 368, 410, 416
MflI RGATCY 1 cut(s) 415
MhlI GDGCHC 3 cut(s) 52, 122, 292
MluCI AATT 3 cut(s) 308, 433, 451
MlyI GAGTC 1 cut(s) 377
MmeI TCCRAC 1 cut(s) 233
MnlI CCTC 8 cut(s) 19, 100, 204, 211, 214, 437, 451, 475
MseI TTAA 1 cut(s) 529
MspA1I CMGCKG 1 cut(s) 37
MspR9I CCNGG 1 cut(s) 13
MvaI CCWGG 1 cut(s) 13
MvnI CGCG 2 cut(s) 230, 377
MwoI GCNNNNNNNGC 4 cut(s) 25, 34, 78, 87
NdeII GATC 6 cut(s) 133, 247, 283, 346, 399, 415
NlaIII CATG 3 cut(s) 173, 245, 494
NlaIV GGNNCC 1 cut(s) 146
NspI RCATGY 2 cut(s) 173, 245
PaeI GCATGC 1 cut(s) 173
PaeR7I CTCGAG 1 cut(s) 479
PagI TCATGA 1 cut(s) 490
PciSI GCTCTTC 1 cut(s) 399
PfeI GAWTC 1 cut(s) 55
PkrI GCNGC 5 cut(s) 18, 36, 39, 80, 229
PleI GAGTC 1 cut(s) 376
PpsI GAGTC 1 cut(s) 376
Psp124BI GAGCTC 2 cut(s) 52, 122
Psp6I CCWGG 1 cut(s) 11
PspGI CCWGG 1 cut(s) 11
PspN4I GGNNCC 1 cut(s) 146
PspXI VCTCGAGB 1 cut(s) 479
PstI CTGCAG 1 cut(s) 83
PstNI CAGNNNCTG 1 cut(s) 78
PsuI RGATCY 1 cut(s) 415
PvuII CAGCTG 1 cut(s) 37
RsaI GTAC 1 cut(s) 146
RsaNI GTAC 1 cut(s) 145
SacI GAGCTC 2 cut(s) 52, 122
SapI GCTCTTC 1 cut(s) 399
SaqAI TTAA 1 cut(s) 529
SatI GCNGC 5 cut(s) 17, 35, 38, 79, 228
Sau3AI GATC 6 cut(s) 133, 247, 283, 346, 399, 415
SchI GAGTC 1 cut(s) 377
ScrFI CCNGG 1 cut(s) 13
SduI GDGCHC 3 cut(s) 52, 122, 292
SetI ASST 8 cut(s) 39, 46, 52, 80, 122, 146, 164, 486
SfaNI GCATC 1 cut(s) 214
SfcI CTRYAG 1 cut(s) 79
Sfr274I CTCGAG 1 cut(s) 479
SlaI CTCGAG 1 cut(s) 479
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
SphI GCATGC 1 cut(s) 173
Sse9I AATT 3 cut(s) 308, 433, 451
SsiI CCGC 3 cut(s) 228, 377, 397
SspMI CTAG 1 cut(s) 191
SstI GAGCTC 2 cut(s) 52, 122
StyD4I CCNGG 1 cut(s) 11
TaiI ACGT 1 cut(s) 46
TaqI TCGA 5 cut(s) 152, 286, 402, 446, 480
TasI AATT 3 cut(s) 308, 433, 451
TauI GCSGC 1 cut(s) 230
TfiI GAWTC 1 cut(s) 55
Tru1I TTAA 1 cut(s) 529
Tru9I TTAA 1 cut(s) 529
TseI GCWGC 4 cut(s) 16, 34, 37, 78
TspDTI ATGAA 3 cut(s) 464, 479, 507
XapI RAATTY 2 cut(s) 308, 451
XceI RCATGY 2 cut(s) 173, 245
XhoI CTCGAG 1 cut(s) 479
XspI CTAG 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.