MD16G1133300.v1.1

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002239
Physical Location & Seq
Reverse (-)
10256219 .. 10256467
249 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1133300.v1.1.491

Sequence Viewer

Length: 249 bp
ATGGGAAAGCAATCTTCACTCTTCTCTTTCTGCAACATGTTCAAGGCCTGCTTTTCAAGCGGCAGCAGAGATGATACATATTACTCCGATGATGGCGGTGTTTATGTTAGACGGAGAATATGTCCCAGTGATGAGGACAGAGGAGGCTGGACTGCCGAGCCTGGCATCGATGGCAAGGCCACTGCTTTCATTTCCAGGTTCTACGCGAGTCGTGTTTCGGATCCTGAATGCCAAACTTTCGCAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF761

Protein Analysis

83

Amino Acids

9.12

Weight (kDa)

5.19

Isoelectric Point (pI)

68.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 206
AciI CCGC 2 cut(s) 60, 96
AclWI GGATC 2 cut(s) 215, 228
AflIII ACRYGT 1 cut(s) 36
AgsI TTSAA 2 cut(s) 43, 57
AjnI CCWGG 2 cut(s) 160, 194
AlwI GGATC 2 cut(s) 215, 228
AoxI GGCC 2 cut(s) 45, 177
ApeKI GCWGC 1 cut(s) 63
BamHI GGATCC 1 cut(s) 220
BbvI GCAGC 1 cut(s) 75
BccI CCATC 2 cut(s) 86, 164
BciT130I CCWGG 2 cut(s) 162, 196
BisI GCNGC 2 cut(s) 61, 64
BlsI GCNGC 2 cut(s) 62, 65
Bme1390I CCNGG 2 cut(s) 162, 196
BmiI GGNNCC 1 cut(s) 222
BmrFI CCNGG 2 cut(s) 162, 196
BmrI ACTGGG 1 cut(s) 120
BmsI GCATC 1 cut(s) 174
BmuI ACTGGG 1 cut(s) 120
Bsa29I ATCGAT 1 cut(s) 168
BsaXI ACNNNNNCTCC 2 cut(s) 106, 136
Bse1I ACTGG 1 cut(s) 126
BseBI CCWGG 2 cut(s) 162, 196
BseCI ATCGAT 1 cut(s) 168
BseNI ACTGG 1 cut(s) 126
BseRI GAGGAG 1 cut(s) 156
BseXI GCAGC 1 cut(s) 75
Bsh1236I CGCG 1 cut(s) 206
BshFI GGCC 2 cut(s) 47, 179
BshVI ATCGAT 1 cut(s) 168
BslFI GGGAC 1 cut(s) 108
BsmFI GGGAC 1 cut(s) 108
BsmI GAATGC 1 cut(s) 233
BsnI GGCC 2 cut(s) 47, 179
Bsp143I GATC 1 cut(s) 220
BspACI CCGC 2 cut(s) 60, 96
BspANI GGCC 2 cut(s) 47, 179
BspDI ATCGAT 1 cut(s) 168
BspFNI CGCG 1 cut(s) 206
BspLI GGNNCC 1 cut(s) 222
BspPI GGATC 2 cut(s) 215, 228
BsrI ACTGG 1 cut(s) 126
BssMI GATC 1 cut(s) 220
Bst2UI CCWGG 2 cut(s) 162, 196
Bst6I CTCTTC 1 cut(s) 26
BstC8I GCNNGC 1 cut(s) 49
BstFNI CGCG 1 cut(s) 206
BstKTI GATC 1 cut(s) 223
BstMBI GATC 1 cut(s) 220
BstMWI GCNNNNNNNGC 2 cut(s) 57, 171
BstNI CCWGG 2 cut(s) 162, 196
BstNSI RCATGY 1 cut(s) 40
BstSCI CCNGG 2 cut(s) 160, 194
BstUI CGCG 1 cut(s) 206
BstV1I GCAGC 1 cut(s) 75
BstX2I RGATCY 1 cut(s) 220
BstYI RGATCY 1 cut(s) 220
Bsu15I ATCGAT 1 cut(s) 168
BsuRI GGCC 2 cut(s) 47, 179
BsuTUI ATCGAT 1 cut(s) 168
BtsI GCAGTG 1 cut(s) 180
BtsIMutI CAGTG 2 cut(s) 133, 180
Cac8I GCNNGC 1 cut(s) 49
ClaI ATCGAT 1 cut(s) 168
CviAII CATG 1 cut(s) 37
CviJI RGCY 4 cut(s) 47, 147, 160, 179
CviKI_1 RGCY 4 cut(s) 47, 147, 160, 179
DpnI GATC 1 cut(s) 222
DpnII GATC 1 cut(s) 220
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
Eco147I AGGCCT 1 cut(s) 47
EcoRII CCWGG 2 cut(s) 160, 194
FaeI CATG 1 cut(s) 40
FaiI YATR 4 cut(s) 38, 79, 105, 121
FalI AAGNNNNNCTT 2 cut(s) 35, 67
FaqI GGGAC 1 cut(s) 108
FatI CATG 1 cut(s) 36
Fnu4HI GCNGC 2 cut(s) 61, 64
Fsp4HI GCNGC 2 cut(s) 61, 64
GluI GCNGC 2 cut(s) 61, 64
HaeIII GGCC 2 cut(s) 47, 179
Hin1II CATG 1 cut(s) 40
HinfI GANTC 1 cut(s) 208
Hpy188I TCNGA 2 cut(s) 88, 220
Hpy188III TCNNGA 1 cut(s) 224
HpyCH4V TGCA 1 cut(s) 33
HpyF10VI GCNNNNNNNGC 2 cut(s) 57, 171
Hsp92II CATG 1 cut(s) 40
Kzo9I GATC 1 cut(s) 220
LpnPI CCDG 8 cut(s) 61, 133, 139, 147, 174, 181, 208, 237
Lsp1109I GCAGC 1 cut(s) 75
LweI GCATC 1 cut(s) 174
MalI GATC 1 cut(s) 222
MboI GATC 1 cut(s) 220
MboII GAAGA 2 cut(s) 6, 13
MflI RGATCY 1 cut(s) 220
MluCI AATT 1 cut(s) 244
MlyI GAGTC 1 cut(s) 217
MnlI CCTC 3 cut(s) 127, 134, 137
MspR9I CCNGG 2 cut(s) 162, 196
Mva1269I GAATGC 1 cut(s) 233
MvaI CCWGG 2 cut(s) 162, 196
MvnI CGCG 1 cut(s) 206
MwoI GCNNNNNNNGC 2 cut(s) 57, 171
NdeII GATC 1 cut(s) 220
NlaIII CATG 1 cut(s) 40
NlaIV GGNNCC 1 cut(s) 222
NmeAIII GCCGAG 1 cut(s) 181
NspI RCATGY 1 cut(s) 40
PceI AGGCCT 1 cut(s) 47
PciI ACATGT 1 cut(s) 36
PctI GAATGC 1 cut(s) 233
PkrI GCNGC 2 cut(s) 62, 65
PleI GAGTC 1 cut(s) 216
PpsI GAGTC 1 cut(s) 216
PscI ACATGT 1 cut(s) 36
Psp6I CCWGG 2 cut(s) 160, 194
PspGI CCWGG 2 cut(s) 160, 194
PspN4I GGNNCC 1 cut(s) 222
PsuI RGATCY 1 cut(s) 220
SatI GCNGC 2 cut(s) 61, 64
Sau3AI GATC 1 cut(s) 220
SchI GAGTC 1 cut(s) 217
ScrFI CCNGG 2 cut(s) 162, 196
SetI ASST 1 cut(s) 200
SfaNI GCATC 1 cut(s) 174
Sse9I AATT 1 cut(s) 244
SseBI AGGCCT 1 cut(s) 47
SsiI CCGC 2 cut(s) 60, 96
StuI AGGCCT 1 cut(s) 47
StyD4I CCNGG 2 cut(s) 160, 194
TaqI TCGA 1 cut(s) 168
TasI AATT 1 cut(s) 244
TauI GCSGC 1 cut(s) 63
TscAI CASTG 2 cut(s) 133, 187
TseI GCWGC 1 cut(s) 63
TspDTI ATGAA 1 cut(s) 178
TspGWI ACGGA 1 cut(s) 127
TspRI CASTG 2 cut(s) 133, 187
XceI RCATGY 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.