Rorug04G0220800

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002239
Physical Location & Seq
Forward (+)
38024791 .. 38026086
1296 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0220800.1

Sequence Viewer

Length: 627 bp
ATGGCTGCGTCTGTTCATCTTGCTCCAGAGCTCTTCCCTCTTGTCATTTCCAGGACAGGGAAGAATAATTTGAGACCTAATAAAGAGTATAAACTGCACTCCAGGTGTGCATTACCTTCTGAAGGAGCTTCATCAACTCGCCGACTTGTAGTATATGCGGGTGCTTCTATCCTTGCGGTTCTCGCCACTAACTATGGGCTAGTGCCATTGCCTGTTTTGGCAGATGACAAGTCAAATGGTGAAGAGGAGAAGGATGGAGTTTTAGGTGCTATCCAGTCGCTGCTCGAAACCAATACGAAAACGAAGTCTGGAAGAGTGTTGCCAAAGGCATATTTGAAGACTGCAAAAGAAGTTGTGAAGACACTGCGGGAATCAGTACAGGAAGATCCCAAGGATAATGCAAAGTTTAGACGTACTGCAGATGCAGCAAAGGAATCAATTCGAGAGTATTTGAGCAATTGGAGGGGACAGCCAACGGTGTCTCAAGAGGAATCTTATGTCGAGTTAGAGAAAGCAATAAGATCATTGGCCGGCTTTTACTCAAAGGCAGGACCATCTGCCCCATTGCCTGAAGAAGTGAAGTCAGAAATATTGAGGGATTTAGATACAGCTGAAAAGTTTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF761

Protein Analysis

208

Amino Acids

22.83

Weight (kDa)

8.84

Isoelectric Point (pI)

51.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSII_Pbs27 PF13326 48 - 208 2e-46 Photosystem II Pbs27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 158, 176, 367
AclWI GGATC 1 cut(s) 380
AcoI YGGCCR 1 cut(s) 528
AcuI CTGAAG 2 cut(s) 141, 591
AfaI GTAC 2 cut(s) 378, 415
AfiI CCNNNNNNNGG 2 cut(s) 57, 122
AgsI TTSAA 1 cut(s) 337
AjnI CCWGG 2 cut(s) 50, 101
AluBI AGCT 3 cut(s) 31, 128, 611
AluI AGCT 3 cut(s) 31, 128, 611
Alw21I GWGCWC 1 cut(s) 33
Alw26I GTCTC 2 cut(s) 67, 486
AlwI GGATC 1 cut(s) 380
AlwNI CAGNNNCTG 1 cut(s) 280
AoxI GGCC 1 cut(s) 528
ApeKI GCWGC 3 cut(s) 5, 280, 425
ArsI GACNNNNNNTTYG 2 cut(s) 290, 322
Asp700I GAANNNNTTC 1 cut(s) 438
AspS9I GGNCC 1 cut(s) 551
AsuHPI GGTGA 1 cut(s) 251
AvaII GGWCC 1 cut(s) 551
BanII GRGCYC 1 cut(s) 33
BbsI GAAGAC 2 cut(s) 344, 365
Bbv12I GWGCWC 1 cut(s) 33
BbvI GCAGC 2 cut(s) 267, 437
BccI CCATC 2 cut(s) 248, 562
BciT130I CCWGG 2 cut(s) 52, 103
BcoDI GTCTC 2 cut(s) 67, 486
BfaI CTAG 1 cut(s) 200
BfmI CTRYAG 1 cut(s) 417
BisI GCNGC 3 cut(s) 6, 281, 426
BlsI GCNGC 3 cut(s) 7, 282, 427
Bme1390I CCNGG 2 cut(s) 52, 103
Bme18I GGWCC 1 cut(s) 551
BmgT120I GGNCC 1 cut(s) 551
BmrFI CCNGG 2 cut(s) 52, 103
BmsI GCATC 1 cut(s) 412
BpiI GAAGAC 2 cut(s) 344, 365
BpmI CTGGAG 2 cut(s) 9, 85
BpuEI CTTGAG 1 cut(s) 468
BsaI GGTCTC 1 cut(s) 67
BsaJI CCNNGG 1 cut(s) 390
Bsc4I CCNNNNNNNGG 2 cut(s) 57, 122
Bse118I RCCGGY 1 cut(s) 530
Bse1I ACTGG 1 cut(s) 274
Bse3DI GCAATG 2 cut(s) 206, 563
BseBI CCWGG 2 cut(s) 52, 103
BseDI CCNNGG 1 cut(s) 390
BseGI GGATG 1 cut(s) 259
BseLI CCNNNNNNNGG 2 cut(s) 57, 122
BseMI GCAATG 2 cut(s) 206, 563
BseNI ACTGG 1 cut(s) 274
BseRI GAGGAG 1 cut(s) 260
BseXI GCAGC 2 cut(s) 267, 437
BsgI GTGCAG 1 cut(s) 80
BshFI GGCC 1 cut(s) 530
BsiHKAI GWGCWC 1 cut(s) 33
BsiSI CCGG 1 cut(s) 531
BslFI GGGAC 1 cut(s) 480
BslI CCNNNNNNNGG 2 cut(s) 57, 122
BsmAI GTCTC 2 cut(s) 67, 486
BsmFI GGGAC 1 cut(s) 480
BsnI GGCC 1 cut(s) 530
Bso31I GGTCTC 1 cut(s) 67
Bsp1286I GDGCHC 1 cut(s) 33
Bsp143I GATC 2 cut(s) 385, 521
BspACI CCGC 3 cut(s) 158, 176, 367
BspANI GGCC 1 cut(s) 530
BspMAI CTGCAG 1 cut(s) 421
BspPI GGATC 1 cut(s) 380
BspQI GCTCTTC 1 cut(s) 38
BspTNI GGTCTC 1 cut(s) 67
BsrDI GCAATG 2 cut(s) 206, 563
BsrFI RCCGGY 1 cut(s) 530
BsrI ACTGG 1 cut(s) 274
BssAI RCCGGY 1 cut(s) 530
BssECI CCNNGG 1 cut(s) 390
BssMI GATC 2 cut(s) 385, 521
BssT1I CCWWGG 1 cut(s) 390
Bst2UI CCWGG 2 cut(s) 52, 103
Bst4CI ACNGT 1 cut(s) 478
Bst6I CTCTTC 3 cut(s) 38, 237, 307
BstC8I GCNNGC 1 cut(s) 532
BstENI CCTNNNNNAGG 1 cut(s) 120
BstF5I GGATG 1 cut(s) 259
BstKTI GATC 2 cut(s) 388, 524
BstMAI GTCTC 2 cut(s) 67, 486
BstMBI GATC 2 cut(s) 385, 521
BstMWI GCNNNNNNNGC 2 cut(s) 182, 425
BstNI CCWGG 2 cut(s) 52, 103
BstSCI CCNGG 2 cut(s) 50, 101
BstSFI CTRYAG 1 cut(s) 417
BstV1I GCAGC 2 cut(s) 267, 437
BstV2I GAAGAC 2 cut(s) 344, 365
BstX2I RGATCY 1 cut(s) 385
BstYI RGATCY 1 cut(s) 385
BsuRI GGCC 1 cut(s) 530
BtsCI GGATG 1 cut(s) 259
BtsI GCAGTG 1 cut(s) 362
BtsIMutI CAGTG 1 cut(s) 362
Cac8I GCNNGC 1 cut(s) 532
CaiI CAGNNNCTG 1 cut(s) 280
Cfr10I RCCGGY 1 cut(s) 530
Cfr13I GGNCC 1 cut(s) 551
Csp6I GTAC 2 cut(s) 377, 414
CviJI RGCY 8 cut(s) 5, 31, 128, 199, 472, 530, 534, 611
CviKI_1 RGCY 8 cut(s) 5, 31, 128, 199, 472, 530, 534, 611
CviQI GTAC 2 cut(s) 377, 414
DpnI GATC 2 cut(s) 387, 523
DpnII GATC 2 cut(s) 385, 521
EaeI YGGCCR 1 cut(s) 528
Eam1104I CTCTTC 3 cut(s) 38, 237, 307
EarI CTCTTC 3 cut(s) 38, 237, 307
Ecl136II GAGCTC 1 cut(s) 31
Eco130I CCWWGG 1 cut(s) 390
Eco24I GRGCYC 1 cut(s) 33
Eco31I GGTCTC 1 cut(s) 67
Eco47I GGWCC 1 cut(s) 551
Eco53kI GAGCTC 1 cut(s) 31
Eco57I CTGAAG 2 cut(s) 141, 591
EcoICRI GAGCTC 1 cut(s) 31
EcoNI CCTNNNNNAGG 1 cut(s) 120
EcoRII CCWGG 2 cut(s) 50, 101
EcoT14I CCWWGG 1 cut(s) 390
EcoT38I GRGCYC 1 cut(s) 33
ErhI CCWWGG 1 cut(s) 390
FaiI YATR 7 cut(s) 90, 154, 156, 195, 331, 498, 625
FaqI GGGAC 1 cut(s) 480
FauI CCCGC 2 cut(s) 151, 360
Fnu4HI GCNGC 3 cut(s) 6, 281, 426
FokI GGATG 1 cut(s) 266
FriOI GRGCYC 1 cut(s) 33
Fsp4HI GCNGC 3 cut(s) 6, 281, 426
FspBI CTAG 1 cut(s) 200
GluI GCNGC 3 cut(s) 6, 281, 426
GsuI CTGGAG 2 cut(s) 9, 85
HaeIII GGCC 1 cut(s) 530
HapII CCGG 1 cut(s) 531
HinfI GANTC 3 cut(s) 371, 434, 491
HpaII CCGG 1 cut(s) 531
HphI GGTGA 1 cut(s) 251
Hpy188I TCNGA 2 cut(s) 121, 586
Hpy188III TCNNGA 4 cut(s) 26, 309, 443, 485
HpyAV CCTTC 3 cut(s) 116, 126, 244
HpyCH4III ACNGT 1 cut(s) 478
HpyCH4IV ACGT 1 cut(s) 412
HpyCH4V TGCA 6 cut(s) 97, 110, 344, 401, 419, 425
HpyF10VI GCNNNNNNNGC 2 cut(s) 182, 425
HpySE526I ACGT 1 cut(s) 412
KroI GCCGGC 1 cut(s) 530
KroNI GCCGGC 1 cut(s) 532
Kzo9I GATC 2 cut(s) 385, 521
LguI GCTCTTC 1 cut(s) 38
LmnI GCTCC 2 cut(s) 28, 125
Lsp1109I GCAGC 2 cut(s) 267, 437
LweI GCATC 1 cut(s) 412
MaeI CTAG 1 cut(s) 200
MaeII ACGT 1 cut(s) 412
MalI GATC 2 cut(s) 387, 523
MboI GATC 2 cut(s) 385, 521
MboII GAAGA 8 cut(s) 25, 73, 254, 324, 349, 370, 395, 584
MfeI CAATTG 1 cut(s) 457
MflI RGATCY 1 cut(s) 385
MhlI GDGCHC 1 cut(s) 33
MluCI AATT 3 cut(s) 67, 438, 457
MnlI CCTC 5 cut(s) 48, 238, 456, 481, 588
MroNI GCCGGC 1 cut(s) 530
MroXI GAANNNNTTC 1 cut(s) 438
MspA1I CMGCKG 1 cut(s) 611
MspI CCGG 1 cut(s) 531
MspR9I CCNGG 2 cut(s) 52, 103
MunI CAATTG 1 cut(s) 457
MvaI CCWGG 2 cut(s) 52, 103
MwoI GCNNNNNNNGC 2 cut(s) 182, 425
NaeI GCCGGC 1 cut(s) 532
NdeII GATC 2 cut(s) 385, 521
NgoMIV GCCGGC 1 cut(s) 530
PciSI GCTCTTC 1 cut(s) 38
PdiI GCCGGC 1 cut(s) 532
PdmI GAANNNNTTC 1 cut(s) 438
PfeI GAWTC 3 cut(s) 371, 434, 491
PfoI TCCNGGA 1 cut(s) 50
PkrI GCNGC 3 cut(s) 7, 282, 427
Psp124BI GAGCTC 1 cut(s) 33
Psp6I CCWGG 2 cut(s) 50, 101
PspGI CCWGG 2 cut(s) 50, 101
PspPI GGNCC 1 cut(s) 551
PstI CTGCAG 1 cut(s) 421
PstNI CAGNNNCTG 1 cut(s) 280
PsuI RGATCY 1 cut(s) 385
PvuII CAGCTG 1 cut(s) 611
RsaI GTAC 2 cut(s) 378, 415
RsaNI GTAC 2 cut(s) 377, 414
SacI GAGCTC 1 cut(s) 33
SapI GCTCTTC 1 cut(s) 38
SatI GCNGC 3 cut(s) 6, 281, 426
Sau3AI GATC 2 cut(s) 385, 521
Sau96I GGNCC 1 cut(s) 551
ScrFI CCNGG 2 cut(s) 52, 103
SduI GDGCHC 1 cut(s) 33
SetI ASST 8 cut(s) 33, 79, 107, 118, 130, 268, 415, 613
SfaNI GCATC 1 cut(s) 412
SfcI CTRYAG 1 cut(s) 417
SinI GGWCC 1 cut(s) 551
SmlI CTYRAG 1 cut(s) 483
SmoI CTYRAG 1 cut(s) 483
Sse9I AATT 3 cut(s) 67, 438, 457
SsiI CCGC 3 cut(s) 158, 176, 367
SspI AATATT 1 cut(s) 591
SspMI CTAG 1 cut(s) 200
SstI GAGCTC 1 cut(s) 33
StyD4I CCNGG 2 cut(s) 50, 101
StyI CCWWGG 1 cut(s) 390
TaaI ACNGT 1 cut(s) 478
TaiI ACGT 1 cut(s) 415
TaqI TCGA 3 cut(s) 285, 442, 501
TasI AATT 3 cut(s) 67, 438, 457
TatI WGTACW 1 cut(s) 376
TfiI GAWTC 3 cut(s) 371, 434, 491
TscAI CASTG 1 cut(s) 369
TseI GCWGC 3 cut(s) 5, 280, 425
TspDTI ATGAA 2 cut(s) 5, 120
TspRI CASTG 1 cut(s) 369
VpaK11BI GGWCC 1 cut(s) 551
XagI CCTNNNNNAGG 1 cut(s) 120
XmnI GAANNNNTTC 1 cut(s) 438
XspI CTAG 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.