MD17G1163800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002459
Physical Location & Seq
Reverse (-)
16205734 .. 16208038
2305 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1163800.v1.1.491

Sequence Viewer

Length: 519 bp
ATGGCAAGGTCGTCAATTTCTCAAACCCTTACCCTAACCCGCCACCTCTCGCCCAAATCATCGCCGTCCTCCTCCCGCCTCATAACCCTGCGCGCCCAATCCACCCTCCCTTTTCACCATGATCCACCAACCGACTCCTCCGCCGACTCGCCTTCTGATCCCCTTCTGCGCAAGCTCGAGGACGCCATCCACCGGATCATCGTCCGCCGATCCGCGCCCGATTGGCTCCCCTTTTTGCCCGGCGCCTCCTACTGGGTCCCTCCTCCACGGTCGAGATCGCACGGCCTGGCTCAGCTGGTCGATAAGTTGGCCAACCCTTTGAGTGAGGAGGAGACCATGTCTATGAGCACCGTTAGAGGGTGGCCTTCTTCTGCTTATTTTATCGAAGGTACATCTCCACAACTGATGGAGCCTGTGGTTGTGTTTCAGAGTTCTGATGATGTGTCCAATCCCCAACCGATGGAGACCGCGGATCAAATTTCTAACAATGTGTCCAAGTCCGAGGATGAGGAGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

173

Amino Acids

18.93

Weight (kDa)

5.29

Isoelectric Point (pI)

87.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 170
AccB1I GGYRCC 1 cut(s) 242
AccB7I CCANNNNNTGG 1 cut(s) 460
AccII CGCG 3 cut(s) 93, 215, 470
AciI CCGC 7 cut(s) 40, 76, 141, 205, 213, 468, 470
AclWI GGATC 5 cut(s) 116, 152, 203, 204, 480
AcoI YGGCCR 1 cut(s) 309
AcsI RAATTY 1 cut(s) 477
AcyI GRCGYC 2 cut(s) 183, 243
AfaI GTAC 1 cut(s) 391
AfiI CCNNNNNNNGG 4 cut(s) 192, 252, 357, 460
AjnI CCWGG 1 cut(s) 285
AluBI AGCT 2 cut(s) 175, 295
AluI AGCT 2 cut(s) 175, 295
Alw21I GWGCWC 1 cut(s) 350
Alw26I GTCTC 2 cut(s) 326, 458
AlwI GGATC 5 cut(s) 116, 152, 203, 204, 480
Ama87I CYCGRG 1 cut(s) 176
AoxI GGCC 3 cut(s) 283, 309, 362
ApoI RAATTY 1 cut(s) 477
AspLEI GCGC 5 cut(s) 93, 95, 171, 217, 245
AspS9I GGNCC 1 cut(s) 256
AsuC2I CCSGG 1 cut(s) 240
AsuHPI GGTGA 1 cut(s) 107
AvaI CYCGRG 1 cut(s) 176
AvaII GGWCC 1 cut(s) 256
BalI TGGCCA 1 cut(s) 311
BanI GGYRCC 1 cut(s) 242
Bbv12I GWGCWC 1 cut(s) 350
BccI CCATC 3 cut(s) 194, 400, 454
BceAI ACGGC 2 cut(s) 49, 298
BciT130I CCWGG 1 cut(s) 287
BcnI CCSGG 1 cut(s) 240
BcoDI GTCTC 2 cut(s) 326, 458
BfoI RGCGCY 1 cut(s) 246
BglI GCCNNNNNGGC 1 cut(s) 223
BlpI GCTNAGC 1 cut(s) 291
Bme1390I CCNGG 2 cut(s) 240, 287
Bme18I GGWCC 1 cut(s) 256
BmeT110I CYCGRG 1 cut(s) 176
BmgT120I GGNCC 1 cut(s) 256
BmiI GGNNCC 5 cut(s) 227, 244, 257, 258, 411
BmrFI CCNGG 2 cut(s) 240, 287
BmrI ACTGGG 1 cut(s) 262
BmuI ACTGGG 1 cut(s) 262
Bpu1102I GCTNAGC 1 cut(s) 291
BpuMI CCSGG 1 cut(s) 240
BsaHI GRCGYC 2 cut(s) 183, 243
BsaI GGTCTC 2 cut(s) 326, 458
BsaJI CCNNGG 3 cut(s) 266, 468, 501
BsaWI WCCGGW 1 cut(s) 192
BsaXI ACNNNNNCTCC 2 cut(s) 323, 353
Bsc4I CCNNNNNNNGG 4 cut(s) 192, 252, 357, 460
Bse1I ACTGG 1 cut(s) 257
BseBI CCWGG 1 cut(s) 287
BseDI CCNNGG 3 cut(s) 266, 468, 501
BseGI GGATG 2 cut(s) 186, 511
BseLI CCNNNNNNNGG 4 cut(s) 192, 252, 357, 460
BseMII CTCAG 1 cut(s) 305
BseNI ACTGG 1 cut(s) 257
BsePI GCGCGC 1 cut(s) 91
BseRI GAGGAG 5 cut(s) 61, 127, 252, 341, 344
Bsh1236I CGCG 3 cut(s) 93, 215, 470
Bsh1285I CGRYCG 1 cut(s) 272
BshFI GGCC 3 cut(s) 285, 311, 364
BshNI GGYRCC 1 cut(s) 242
BsiEI CGRYCG 1 cut(s) 272
BsiHKAI GWGCWC 1 cut(s) 350
BsiHKCI CYCGRG 1 cut(s) 176
BsiSI CCGG 2 cut(s) 193, 240
BslFI GGGAC 1 cut(s) 242
BslI CCNNNNNNNGG 4 cut(s) 192, 252, 357, 460
BsmAI GTCTC 2 cut(s) 326, 458
BsmFI GGGAC 1 cut(s) 242
BsnI GGCC 3 cut(s) 285, 311, 364
Bso31I GGTCTC 2 cut(s) 326, 458
BsoBI CYCGRG 1 cut(s) 176
Bsp1286I GDGCHC 1 cut(s) 350
Bsp143I GATC 6 cut(s) 121, 157, 195, 209, 275, 472
Bsp1720I GCTNAGC 1 cut(s) 291
BspACI CCGC 7 cut(s) 40, 76, 141, 205, 213, 468, 470
BspANI GGCC 3 cut(s) 285, 311, 364
BspCNI CTCAG 1 cut(s) 304
BspFNI CGCG 3 cut(s) 93, 215, 470
BspLI GGNNCC 5 cut(s) 227, 244, 257, 258, 411
BspPI GGATC 5 cut(s) 116, 152, 203, 204, 480
BspT107I GGYRCC 1 cut(s) 242
BspTNI GGTCTC 2 cut(s) 326, 458
BsrI ACTGG 1 cut(s) 257
BssECI CCNNGG 3 cut(s) 266, 468, 501
BssHII GCGCGC 1 cut(s) 91
BssMI GATC 6 cut(s) 121, 157, 195, 209, 275, 472
BssNI GRCGYC 2 cut(s) 183, 243
Bst2UI CCWGG 1 cut(s) 287
Bst4CI ACNGT 2 cut(s) 270, 352
BstACI GRCGYC 2 cut(s) 183, 243
BstC8I GCNNGC 2 cut(s) 93, 173
BstDEI CTNAG 1 cut(s) 291
BstDSI CCRYGG 2 cut(s) 266, 468
BstF5I GGATG 2 cut(s) 186, 511
BstFNI CGCG 3 cut(s) 93, 215, 470
BstH2I RGCGCY 1 cut(s) 246
BstHHI GCGC 5 cut(s) 93, 95, 171, 217, 245
BstKTI GATC 6 cut(s) 124, 160, 198, 212, 278, 475
BstMAI GTCTC 2 cut(s) 326, 458
BstMBI GATC 6 cut(s) 121, 157, 195, 209, 275, 472
BstMCI CGRYCG 1 cut(s) 272
BstMWI GCNNNNNNNGC 1 cut(s) 223
BstNI CCWGG 1 cut(s) 287
BstSCI CCNGG 2 cut(s) 238, 285
BstUI CGCG 3 cut(s) 93, 215, 470
BsuRI GGCC 3 cut(s) 285, 311, 364
BtgI CCRYGG 2 cut(s) 266, 468
BtgZI GCGATG 1 cut(s) 45
BtsCI GGATG 2 cut(s) 186, 511
Cac8I GCNNGC 2 cut(s) 93, 173
CfoI GCGC 5 cut(s) 93, 95, 171, 217, 245
Cfr13I GGNCC 1 cut(s) 256
Cfr42I CCGCGG 1 cut(s) 471
CseI GACGC 1 cut(s) 191
Csp6I GTAC 1 cut(s) 390
CviAII CATG 2 cut(s) 119, 337
CviJI RGCY 8 cut(s) 175, 226, 285, 290, 295, 311, 364, 412
CviKI_1 RGCY 8 cut(s) 175, 226, 285, 290, 295, 311, 364, 412
CviQI GTAC 1 cut(s) 390
DdeI CTNAG 1 cut(s) 291
DinI GGCGCC 1 cut(s) 244
DpnI GATC 6 cut(s) 123, 159, 197, 211, 277, 474
DpnII GATC 6 cut(s) 121, 157, 195, 209, 275, 472
EaeI YGGCCR 1 cut(s) 309
EciI GGCGGA 2 cut(s) 130, 194
Eco31I GGTCTC 2 cut(s) 326, 458
Eco47I GGWCC 1 cut(s) 256
Eco88I CYCGRG 1 cut(s) 176
EcoO109I RGGNCCY 1 cut(s) 256
EcoRII CCWGG 1 cut(s) 285
EgeI GGCGCC 1 cut(s) 244
EheI GGCGCC 1 cut(s) 244
FaeI CATG 2 cut(s) 122, 340
FaiI YATR 4 cut(s) 83, 120, 338, 344
FaqI GGGAC 1 cut(s) 242
FatI CATG 2 cut(s) 118, 336
FauI CCCGC 2 cut(s) 47, 83
FokI GGATG 1 cut(s) 173
FspI TGCGCA 1 cut(s) 170
GlaI GCGC 5 cut(s) 92, 94, 170, 216, 244
HaeII RGCGCY 1 cut(s) 246
HaeIII GGCC 3 cut(s) 285, 311, 364
HapII CCGG 2 cut(s) 193, 240
HgaI GACGC 1 cut(s) 191
HhaI GCGC 5 cut(s) 93, 95, 171, 217, 245
Hin1I GRCGYC 2 cut(s) 183, 243
Hin1II CATG 2 cut(s) 122, 340
Hin6I GCGC 5 cut(s) 91, 93, 169, 215, 243
HinP1I GCGC 5 cut(s) 91, 93, 169, 215, 243
HinfI GANTC 2 cut(s) 134, 146
HpaII CCGG 2 cut(s) 193, 240
HphI GGTGA 1 cut(s) 107
Hpy188I TCNGA 4 cut(s) 157, 429, 436, 502
Hpy188III TCNNGA 1 cut(s) 273
HpyAV CCTTC 4 cut(s) 162, 173, 375, 380
HpyCH4III ACNGT 2 cut(s) 270, 352
HpyF10VI GCNNNNNNNGC 1 cut(s) 223
HpyF3I CTNAG 1 cut(s) 291
Hsp92I GRCGYC 2 cut(s) 183, 243
Hsp92II CATG 2 cut(s) 122, 340
HspAI GCGC 5 cut(s) 91, 93, 169, 215, 243
KasI GGCGCC 1 cut(s) 242
KflI GGGWCCC 1 cut(s) 256
KspI CCGCGG 1 cut(s) 471
Kzo9I GATC 6 cut(s) 121, 157, 195, 209, 275, 472
LmnI GCTCC 2 cut(s) 231, 409
LpnPI CCDG 8 cut(s) 101, 206, 238, 253, 272, 281, 299, 426
MalI GATC 6 cut(s) 123, 159, 197, 211, 277, 474
MboI GATC 6 cut(s) 121, 157, 195, 209, 275, 472
MboII GAAGA 1 cut(s) 360
MhlI GDGCHC 1 cut(s) 350
MlsI TGGCCA 1 cut(s) 311
MluCI AATT 2 cut(s) 15, 477
MluNI TGGCCA 1 cut(s) 311
Mly113I GGCGCC 1 cut(s) 243
MlyI GAGTC 2 cut(s) 128, 140
Mox20I TGGCCA 1 cut(s) 311
MscI TGGCCA 1 cut(s) 311
MslI CAYNNNNRTG 1 cut(s) 341
Msp20I TGGCCA 1 cut(s) 311
MspA1I CMGCKG 2 cut(s) 295, 470
MspI CCGG 2 cut(s) 193, 240
MspR9I CCNGG 2 cut(s) 240, 287
MvaI CCWGG 1 cut(s) 287
MvnI CGCG 3 cut(s) 93, 215, 470
MwoI GCNNNNNNNGC 1 cut(s) 223
NarI GGCGCC 1 cut(s) 243
NciI CCSGG 1 cut(s) 240
NdeII GATC 6 cut(s) 121, 157, 195, 209, 275, 472
NlaIII CATG 2 cut(s) 122, 340
NlaIV GGNNCC 5 cut(s) 227, 244, 257, 258, 411
NsbI TGCGCA 1 cut(s) 170
PaeR7I CTCGAG 1 cut(s) 176
PauI GCGCGC 1 cut(s) 91
PflFI GACNNNGTC 1 cut(s) 337
PflMI CCANNNNNTGG 1 cut(s) 460
PleI GAGTC 2 cut(s) 128, 140
PluTI GGCGCC 1 cut(s) 246
PpsI GAGTC 2 cut(s) 128, 140
PpuMI RGGWCCY 1 cut(s) 256
Psp5II RGGWCCY 1 cut(s) 256
Psp6I CCWGG 1 cut(s) 285
PspGI CCWGG 1 cut(s) 285
PspN4I GGNNCC 5 cut(s) 227, 244, 257, 258, 411
PspPI GGNCC 1 cut(s) 256
PspPPI RGGWCCY 1 cut(s) 256
PspXI VCTCGAGB 1 cut(s) 176
PsyI GACNNNGTC 1 cut(s) 337
PteI GCGCGC 1 cut(s) 91
PvuII CAGCTG 1 cut(s) 295
RsaI GTAC 1 cut(s) 391
RsaNI GTAC 1 cut(s) 390
RseI CAYNNNNRTG 1 cut(s) 341
SacII CCGCGG 1 cut(s) 471
Sau3AI GATC 6 cut(s) 121, 157, 195, 209, 275, 472
Sau96I GGNCC 1 cut(s) 256
SchI GAGTC 2 cut(s) 128, 140
ScrFI CCNGG 2 cut(s) 240, 287
SduI GDGCHC 1 cut(s) 350
SetI ASST 5 cut(s) 11, 48, 177, 297, 391
SfoI GGCGCC 1 cut(s) 244
Sfr274I CTCGAG 1 cut(s) 176
Sfr303I CCGCGG 1 cut(s) 471
SgrBI CCGCGG 1 cut(s) 471
SinI GGWCC 1 cut(s) 256
SlaI CTCGAG 1 cut(s) 176
SmiMI CAYNNNNRTG 1 cut(s) 341
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
Sse9I AATT 2 cut(s) 15, 477
SsiI CCGC 7 cut(s) 40, 76, 141, 205, 213, 468, 470
SspDI GGCGCC 1 cut(s) 242
StyD4I CCNGG 2 cut(s) 238, 285
TaaI ACNGT 2 cut(s) 270, 352
TaqI TCGA 4 cut(s) 177, 272, 300, 384
TasI AATT 2 cut(s) 15, 477
Tth111I GACNNNGTC 1 cut(s) 337
Van91I CCANNNNNTGG 1 cut(s) 460
VpaK11BI GGWCC 1 cut(s) 256
XapI RAATTY 1 cut(s) 477
XhoI CTCGAG 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.