Rorug02G0413400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002459
Physical Location & Seq
Reverse (-)
52902817 .. 52905974
3158 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0413400.1

Sequence Viewer

Length: 396 bp
ATGTCGTGGCAGGCTTACGTCGACGATCACTTGATGTGCGACATCGACGGCCACCATCTCGCCTCCGCGGCCATCGTCGGTCACGACGGTAGTGTCTGGGCTCAGAGCTCCAACTTCCCTAAGTTTAAGCCTGAAGAGATTACAGCGATTATGAAAGATTTTAATGAGCCTGGGTCTCTTGCACCAACTGGTCTGCACCTTGCTGGCACAAAGTACATGGTTATTCAAGGGGAGGGCGGAGCTGTTATTCGTGGGAAGAAGGGATCCGGAGGTGTTACTGTGAAGAAAACTGCCCAGGCTTTGATTTTTGGTATATATGAAGAGCCTTTGACACCAGGACAGTGTAATATGATTGTGGAGAGGTTGGGAGATTACCTGATTGATCAGGGCCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

131

Amino Acids

14.06

Weight (kDa)

5.4

Isoelectric Point (pI)

23.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Profilin PF00235 1 - 131 3.1e-45 Profilin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 92
AccI GTMKAC 1 cut(s) 21
AccII CGCG 1 cut(s) 68
AccIII TCCGGA 1 cut(s) 266
AciI CCGC 3 cut(s) 66, 68, 237
AclWI GGATC 2 cut(s) 258, 271
AcoI YGGCCR 2 cut(s) 49, 69
AcuI CTGAAG 1 cut(s) 153
AfaI GTAC 1 cut(s) 215
AgsI TTSAA 1 cut(s) 227
AjnI CCWGG 3 cut(s) 169, 294, 334
AluBI AGCT 2 cut(s) 108, 242
AluI AGCT 2 cut(s) 108, 242
Alw21I GWGCWC 1 cut(s) 110
Alw26I GTCTC 1 cut(s) 180
AlwI GGATC 2 cut(s) 258, 271
AlwNI CAGNNNCTG 1 cut(s) 391
Aor13HI TCCGGA 1 cut(s) 266
AoxI GGCC 3 cut(s) 49, 69, 388
AspS9I GGNCC 1 cut(s) 388
BamHI GGATCC 1 cut(s) 263
BanII GRGCYC 2 cut(s) 103, 110
Bbv12I GWGCWC 1 cut(s) 110
BccI CCATC 2 cut(s) 63, 80
BceAI ACGGC 1 cut(s) 64
BciT130I CCWGG 3 cut(s) 171, 296, 336
BclI TGATCA 1 cut(s) 382
BcoDI GTCTC 1 cut(s) 180
BfmI CTRYAG 1 cut(s) 392
BglI GCCNNNNNGGC 1 cut(s) 68
BisI GCNGC 1 cut(s) 69
BlsI GCNGC 1 cut(s) 70
Bme1390I CCNGG 3 cut(s) 171, 296, 336
BmgT120I GGNCC 1 cut(s) 388
BmiI GGNNCC 1 cut(s) 265
BmrFI CCNGG 3 cut(s) 171, 296, 336
BsaI GGTCTC 1 cut(s) 180
BsaJI CCNNGG 3 cut(s) 66, 170, 294
BsaWI WCCGGW 1 cut(s) 266
Bse1I ACTGG 1 cut(s) 193
BseAI TCCGGA 1 cut(s) 266
BseBI CCWGG 3 cut(s) 171, 296, 336
BseDI CCNNGG 3 cut(s) 66, 170, 294
BseMII CTCAG 1 cut(s) 116
BseNI ACTGG 1 cut(s) 193
BsgI GTGCAG 1 cut(s) 179
Bsh1236I CGCG 1 cut(s) 68
BshFI GGCC 3 cut(s) 51, 71, 390
BsiHKAI GWGCWC 1 cut(s) 110
BsiSI CCGG 1 cut(s) 267
BsmAI GTCTC 1 cut(s) 180
BsnI GGCC 3 cut(s) 51, 71, 390
Bso31I GGTCTC 1 cut(s) 180
Bsp1286I GDGCHC 2 cut(s) 103, 110
Bsp13I TCCGGA 1 cut(s) 266
Bsp143I GATC 3 cut(s) 25, 263, 382
BspACI CCGC 3 cut(s) 66, 68, 237
BspANI GGCC 3 cut(s) 51, 71, 390
BspCNI CTCAG 1 cut(s) 115
BspEI TCCGGA 1 cut(s) 266
BspFNI CGCG 1 cut(s) 68
BspLI GGNNCC 1 cut(s) 265
BspPI GGATC 2 cut(s) 258, 271
BspQI GCTCTTC 1 cut(s) 315
BspTNI GGTCTC 1 cut(s) 180
BsrI ACTGG 1 cut(s) 193
BssECI CCNNGG 3 cut(s) 66, 170, 294
BssMI GATC 3 cut(s) 25, 263, 382
Bst2UI CCWGG 3 cut(s) 171, 296, 336
Bst4CI ACNGT 3 cut(s) 89, 280, 342
Bst6I CTCTTC 2 cut(s) 129, 315
BstC8I GCNNGC 2 cut(s) 12, 205
BstDEI CTNAG 2 cut(s) 102, 120
BstDSI CCRYGG 1 cut(s) 66
BstFNI CGCG 1 cut(s) 68
BstKTI GATC 3 cut(s) 28, 266, 385
BstMAI GTCTC 1 cut(s) 180
BstMBI GATC 3 cut(s) 25, 263, 382
BstMWI GCNNNNNNNGC 1 cut(s) 68
BstNI CCWGG 3 cut(s) 171, 296, 336
BstSCI CCNGG 3 cut(s) 169, 294, 334
BstSFI CTRYAG 1 cut(s) 392
BstUI CGCG 1 cut(s) 68
BstX2I RGATCY 1 cut(s) 263
BstYI RGATCY 1 cut(s) 263
BsuRI GGCC 3 cut(s) 51, 71, 390
BtgI CCRYGG 1 cut(s) 66
BtsIMutI CAGTG 1 cut(s) 347
Cac8I GCNNGC 2 cut(s) 12, 205
CaiI CAGNNNCTG 1 cut(s) 391
Cfr13I GGNCC 1 cut(s) 388
Cfr42I CCGCGG 1 cut(s) 69
Csp6I GTAC 1 cut(s) 214
CviAII CATG 1 cut(s) 217
CviQI GTAC 1 cut(s) 214
DdeI CTNAG 2 cut(s) 102, 120
DpnI GATC 3 cut(s) 27, 265, 384
DpnII GATC 3 cut(s) 25, 263, 382
DrdI GACNNNNNNGTC 1 cut(s) 92
DseDI GACNNNNNNGTC 1 cut(s) 92
EaeI YGGCCR 2 cut(s) 49, 69
Eam1104I CTCTTC 2 cut(s) 129, 315
EarI CTCTTC 2 cut(s) 129, 315
EciI GGCGGA 1 cut(s) 252
Ecl136II GAGCTC 1 cut(s) 108
Eco24I GRGCYC 2 cut(s) 103, 110
Eco31I GGTCTC 1 cut(s) 180
Eco53kI GAGCTC 1 cut(s) 108
Eco57I CTGAAG 1 cut(s) 153
EcoICRI GAGCTC 1 cut(s) 108
EcoO109I RGGNCCY 1 cut(s) 388
EcoRII CCWGG 3 cut(s) 169, 294, 334
EcoT38I GRGCYC 2 cut(s) 103, 110
FaeI CATG 1 cut(s) 220
FaiI YATR 6 cut(s) 152, 218, 314, 316, 318, 350
FatI CATG 1 cut(s) 216
FbaI TGATCA 1 cut(s) 382
FblI GTMKAC 1 cut(s) 21
Fnu4HI GCNGC 1 cut(s) 69
FriOI GRGCYC 2 cut(s) 103, 110
Fsp4HI GCNGC 1 cut(s) 69
GluI GCNGC 1 cut(s) 69
HaeIII GGCC 3 cut(s) 51, 71, 390
HapII CCGG 1 cut(s) 267
Hin1II CATG 1 cut(s) 220
HincII GTYRAC 1 cut(s) 22
HindII GTYRAC 1 cut(s) 22
HpaII CCGG 1 cut(s) 267
Hpy166II GTNNAC 1 cut(s) 22
Hpy188I TCNGA 1 cut(s) 105
Hpy188III TCNNGA 2 cut(s) 83, 267
Hpy8I GTNNAC 1 cut(s) 22
Hpy99I CGWCG 5 cut(s) 23, 26, 50, 80, 89
HpyAV CCTTC 1 cut(s) 253
HpyCH4III ACNGT 3 cut(s) 89, 280, 342
HpyCH4IV ACGT 1 cut(s) 18
HpyCH4V TGCA 2 cut(s) 182, 196
HpyF10VI GCNNNNNNNGC 1 cut(s) 68
HpyF3I CTNAG 2 cut(s) 102, 120
HpySE526I ACGT 1 cut(s) 18
Hsp92II CATG 1 cut(s) 220
Kpn2I TCCGGA 1 cut(s) 266
Ksp22I TGATCA 1 cut(s) 382
KspI CCGCGG 1 cut(s) 69
Kzo9I GATC 3 cut(s) 25, 263, 382
LguI GCTCTTC 1 cut(s) 315
LmnI GCTCC 2 cut(s) 113, 239
MaeII ACGT 1 cut(s) 18
MaeIII GTNAC 2 cut(s) 80, 274
MalI GATC 3 cut(s) 27, 265, 384
MboI GATC 3 cut(s) 25, 263, 382
MboII GAAGA 4 cut(s) 146, 268, 295, 332
MflI RGATCY 1 cut(s) 263
MhlI GDGCHC 2 cut(s) 103, 110
MmeI TCCRAC 1 cut(s) 135
MnlI CCTC 4 cut(s) 73, 226, 263, 354
MroI TCCGGA 1 cut(s) 266
MseI TTAA 2 cut(s) 126, 162
MspA1I CMGCKG 1 cut(s) 68
MspI CCGG 1 cut(s) 267
MspR9I CCNGG 3 cut(s) 171, 296, 336
MvaI CCWGG 3 cut(s) 171, 296, 336
MvnI CGCG 1 cut(s) 68
MwoI GCNNNNNNNGC 1 cut(s) 68
NdeII GATC 3 cut(s) 25, 263, 382
NlaIII CATG 1 cut(s) 220
NlaIV GGNNCC 1 cut(s) 265
NmuCI GTSAC 1 cut(s) 80
PciSI GCTCTTC 1 cut(s) 315
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PkrI GCNGC 1 cut(s) 70
Psp124BI GAGCTC 1 cut(s) 110
Psp6I CCWGG 3 cut(s) 169, 294, 334
PspGI CCWGG 3 cut(s) 169, 294, 334
PspN4I GGNNCC 1 cut(s) 265
PspPI GGNCC 1 cut(s) 388
PstNI CAGNNNCTG 1 cut(s) 391
PsuI RGATCY 1 cut(s) 263
RsaI GTAC 1 cut(s) 215
RsaNI GTAC 1 cut(s) 214
SacI GAGCTC 1 cut(s) 110
SacII CCGCGG 1 cut(s) 69
SalI GTCGAC 1 cut(s) 20
SapI GCTCTTC 1 cut(s) 315
SaqAI TTAA 2 cut(s) 126, 162
SatI GCNGC 1 cut(s) 69
Sau3AI GATC 3 cut(s) 25, 263, 382
Sau96I GGNCC 1 cut(s) 388
ScrFI CCNGG 3 cut(s) 171, 296, 336
SduI GDGCHC 2 cut(s) 103, 110
SetI ASST 7 cut(s) 21, 110, 201, 244, 274, 365, 378
SfcI CTRYAG 1 cut(s) 392
Sfr303I CCGCGG 1 cut(s) 69
SgrBI CCGCGG 1 cut(s) 69
SgrDI CGTCGACG 1 cut(s) 20
SsiI CCGC 3 cut(s) 66, 68, 237
SstI GAGCTC 1 cut(s) 110
StyD4I CCNGG 3 cut(s) 169, 294, 334
TaaI ACNGT 3 cut(s) 89, 280, 342
TaiI ACGT 1 cut(s) 21
TaqI TCGA 2 cut(s) 21, 45
TaqII GACCGA 1 cut(s) 68
TatI WGTACW 1 cut(s) 213
TauI GCSGC 1 cut(s) 71
Tru1I TTAA 2 cut(s) 126, 162
Tru9I TTAA 2 cut(s) 126, 162
TscAI CASTG 1 cut(s) 347
TseFI GTSAC 1 cut(s) 80
Tsp45I GTSAC 1 cut(s) 80
TspDTI ATGAA 2 cut(s) 167, 333
TspRI CASTG 1 cut(s) 347
XmiI GTMKAC 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.