pycom13g21420

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0002459
Physical Location & Seq
Forward (+)
18070811 .. 18072131
1321 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g21420.2

Sequence Viewer

Length: 639 bp
ATGCCGATACTTAAAAAACCTTCCAAAACTCCCTCTCCTTTCACACTCTCTCTCTCTCTCTCAATCAATCTCTCCCGAATTACACAGTTCCCACAGTCCACCGACCAAATGGCTCGACTCCTCACTCAAGCTCTCCATCTCCGCCACTCCCTCCTGCGTCGCCCTCTCCTCCCTCCCTATCTCACCCAGGCCCACCGCGCTCGCTCCTTCCGATCCGGCGAGGCCGAGTTAATCGAGATCGAAGTCGAGCCCTCCCCCTCGTCGTCGCCTAGTGATTCAGAGAAATTAATGCTGAAGAAGCTGGACGACATCGTTCAGACTATCGTCGTCCAGAGGGCCACCCCCGATTGGCTCCCCTTCGTTCCCGGCTCCTCGTTCTGGGTCCCACCTCGACACGCTCCGTTGAAAGTGGCCGACTTGATTGGCAAATTGGCTAACCAGCTCACATTTGAAGAAATCCTCTCTGTTGCTACCGATCGAGGATGGCCTTGCTCCCAATTTTTCATCAATGGCGGCATTGGAACTGCAGAAACAAGGGAGGTGGATACGGAGGCAGAAGGATCAACACCAATAGAGGTGGAGGTGGAGGTCAAGGTTTTGACTGATTCCCAGAAGTCATCTCGCTGCGAGGATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

213

Amino Acids

23.57

Weight (kDa)

5.64

Isoelectric Point (pI)

59.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 198
AciI CCGC 3 cut(s) 142, 196, 513
AclWI GGATC 2 cut(s) 207, 568
AcoI YGGCCR 1 cut(s) 411
AcuI CTGAAG 1 cut(s) 314
AfiI CCNNNNNNNGG 2 cut(s) 348, 378
AgsI TTSAA 2 cut(s) 406, 452
AjnI CCWGG 1 cut(s) 186
AluBI AGCT 3 cut(s) 131, 301, 442
AluI AGCT 3 cut(s) 131, 301, 442
AlwI GGATC 2 cut(s) 207, 568
AoxI GGCC 5 cut(s) 189, 222, 336, 411, 485
ApeKI GCWGC 1 cut(s) 624
AseI ATTAAT 1 cut(s) 287
AspLEI GCGC 1 cut(s) 200
AspS9I GGNCC 3 cut(s) 190, 336, 382
AsuC2I CCSGG 1 cut(s) 366
AsuHPI GGTGA 1 cut(s) 175
AvaII GGWCC 1 cut(s) 382
BanII GRGCYC 1 cut(s) 252
BbvI GCAGC 1 cut(s) 611
BccI CCATC 2 cut(s) 144, 477
BciT130I CCWGG 1 cut(s) 188
BciVI GTATCC 1 cut(s) 538
BcnI CCSGG 1 cut(s) 366
BfaI CTAG 1 cut(s) 270
BfmI CTRYAG 1 cut(s) 525
BfuI GTATCC 1 cut(s) 538
BisI GCNGC 2 cut(s) 514, 625
BlsI GCNGC 2 cut(s) 515, 626
Bme1390I CCNGG 2 cut(s) 188, 366
Bme18I GGWCC 1 cut(s) 382
BmgT120I GGNCC 3 cut(s) 190, 336, 382
BmiI GGNNCC 4 cut(s) 353, 370, 383, 384
BmrFI CCNGG 2 cut(s) 188, 366
BoxI GACNNNNGTC 1 cut(s) 323
BpuEI CTTGAG 1 cut(s) 111
BpuMI CCSGG 1 cut(s) 366
BsaJI CCNNGG 1 cut(s) 186
BsaXI ACNNNNNCTCC 2 cut(s) 19, 49
Bsc4I CCNNNNNNNGG 2 cut(s) 348, 378
BseBI CCWGG 1 cut(s) 188
BseDI CCNNGG 1 cut(s) 186
BseGI GGATG 2 cut(s) 488, 637
BseLI CCNNNNNNNGG 2 cut(s) 348, 378
BseRI GAGGAG 3 cut(s) 110, 158, 361
BseXI GCAGC 1 cut(s) 611
Bsh1236I CGCG 1 cut(s) 198
Bsh1285I CGRYCG 1 cut(s) 478
BshFI GGCC 5 cut(s) 191, 224, 338, 413, 487
BsiEI CGRYCG 1 cut(s) 478
BsiSI CCGG 2 cut(s) 216, 366
BslFI GGGAC 1 cut(s) 368
BslI CCNNNNNNNGG 2 cut(s) 348, 378
BsmFI GGGAC 1 cut(s) 368
BsnI GGCC 5 cut(s) 191, 224, 338, 413, 487
Bsp1286I GDGCHC 1 cut(s) 252
Bsp143I GATC 4 cut(s) 212, 237, 475, 560
BspACI CCGC 3 cut(s) 142, 196, 513
BspANI GGCC 5 cut(s) 191, 224, 338, 413, 487
BspFNI CGCG 1 cut(s) 198
BspLI GGNNCC 4 cut(s) 353, 370, 383, 384
BspMAI CTGCAG 1 cut(s) 529
BspPI GGATC 2 cut(s) 207, 568
BssECI CCNNGG 1 cut(s) 186
BssMI GATC 4 cut(s) 212, 237, 475, 560
Bst2UI CCWGG 1 cut(s) 188
Bst4CI ACNGT 2 cut(s) 87, 96
BstC8I GCNNGC 1 cut(s) 202
BstF5I GGATG 2 cut(s) 488, 637
BstFNI CGCG 1 cut(s) 198
BstHHI GCGC 1 cut(s) 200
BstKTI GATC 4 cut(s) 215, 240, 478, 563
BstMBI GATC 4 cut(s) 212, 237, 475, 560
BstMCI CGRYCG 1 cut(s) 478
BstMWI GCNNNNNNNGC 2 cut(s) 197, 298
BstNI CCWGG 1 cut(s) 188
BstPAI GACNNNNGTC 1 cut(s) 323
BstSCI CCNGG 2 cut(s) 186, 364
BstSFI CTRYAG 1 cut(s) 525
BstUI CGCG 1 cut(s) 198
BstV1I GCAGC 1 cut(s) 611
BsuI GTATCC 1 cut(s) 538
BsuRI GGCC 5 cut(s) 191, 224, 338, 413, 487
BtsCI GGATG 2 cut(s) 488, 637
Cac8I GCNNGC 1 cut(s) 202
CfoI GCGC 1 cut(s) 200
Cfr13I GGNCC 3 cut(s) 190, 336, 382
CseI GACGC 1 cut(s) 146
CspCI CAANNNNNGTGG 4 cut(s) 522, 557, 558, 593
DpnI GATC 4 cut(s) 214, 239, 477, 562
DpnII GATC 4 cut(s) 212, 237, 475, 560
EaeI YGGCCR 1 cut(s) 411
EciI GGCGGA 1 cut(s) 131
Eco24I GRGCYC 1 cut(s) 252
Eco47I GGWCC 1 cut(s) 382
Eco57I CTGAAG 1 cut(s) 314
EcoO109I RGGNCCY 1 cut(s) 382
EcoRII CCWGG 1 cut(s) 186
EcoT38I GRGCYC 1 cut(s) 252
FaqI GGGAC 1 cut(s) 368
Fnu4HI GCNGC 2 cut(s) 514, 625
FokI GGATG 1 cut(s) 495
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 2 cut(s) 514, 625
FspBI CTAG 1 cut(s) 270
GlaI GCGC 1 cut(s) 199
GluI GCNGC 2 cut(s) 514, 625
HaeIII GGCC 5 cut(s) 191, 224, 338, 413, 487
HapII CCGG 2 cut(s) 216, 366
HgaI GACGC 1 cut(s) 146
HhaI GCGC 1 cut(s) 200
Hin6I GCGC 1 cut(s) 198
HinP1I GCGC 1 cut(s) 198
HinfI GANTC 3 cut(s) 117, 275, 605
HpaII CCGG 2 cut(s) 216, 366
HphI GGTGA 1 cut(s) 175
Hpy166II GTNNAC 1 cut(s) 99
Hpy188I TCNGA 3 cut(s) 212, 280, 318
Hpy188III TCNNGA 3 cut(s) 75, 235, 331
Hpy8I GTNNAC 1 cut(s) 99
Hpy99I CGWCG 4 cut(s) 162, 265, 268, 329
HpyAV CCTTC 4 cut(s) 30, 217, 367, 551
HpyCH4III ACNGT 2 cut(s) 87, 96
HpyCH4V TGCA 1 cut(s) 527
HpyF10VI GCNNNNNNNGC 2 cut(s) 197, 298
HspAI GCGC 1 cut(s) 198
KflI GGGWCCC 1 cut(s) 382
Kzo9I GATC 4 cut(s) 212, 237, 475, 560
LmnI GCTCC 5 cut(s) 209, 357, 374, 403, 497
Lsp1109I GCAGC 1 cut(s) 611
MaeI CTAG 1 cut(s) 270
MalI GATC 4 cut(s) 214, 239, 477, 562
MboI GATC 4 cut(s) 212, 237, 475, 560
MboII GAAGA 2 cut(s) 307, 464
MhlI GDGCHC 1 cut(s) 252
MluCI AATT 4 cut(s) 78, 284, 428, 497
MlyI GAGTC 1 cut(s) 111
MseI TTAA 4 cut(s) 12, 230, 287, 637
MspI CCGG 2 cut(s) 216, 366
MspR9I CCNGG 2 cut(s) 188, 366
MvaI CCWGG 1 cut(s) 188
MvnI CGCG 1 cut(s) 198
MwoI GCNNNNNNNGC 2 cut(s) 197, 298
NciI CCSGG 1 cut(s) 366
NdeII GATC 4 cut(s) 212, 237, 475, 560
NlaIV GGNNCC 4 cut(s) 353, 370, 383, 384
NmeAIII GCCGAG 1 cut(s) 250
PcsI WCGNNNNNNNCGW 1 cut(s) 208
PfeI GAWTC 2 cut(s) 275, 605
PkrI GCNGC 2 cut(s) 515, 626
Ple19I CGATCG 1 cut(s) 478
PleI GAGTC 1 cut(s) 111
PpsI GAGTC 1 cut(s) 111
PpuMI RGGWCCY 1 cut(s) 382
PshAI GACNNNNGTC 1 cut(s) 323
PshBI ATTAAT 1 cut(s) 287
Psp5II RGGWCCY 1 cut(s) 382
Psp6I CCWGG 1 cut(s) 186
PspGI CCWGG 1 cut(s) 186
PspN4I GGNNCC 4 cut(s) 353, 370, 383, 384
PspPI GGNCC 3 cut(s) 190, 336, 382
PspPPI RGGWCCY 1 cut(s) 382
PstI CTGCAG 1 cut(s) 529
PvuI CGATCG 1 cut(s) 478
SaqAI TTAA 4 cut(s) 12, 230, 287, 637
SatI GCNGC 2 cut(s) 514, 625
Sau3AI GATC 4 cut(s) 212, 237, 475, 560
Sau96I GGNCC 3 cut(s) 190, 336, 382
SchI GAGTC 1 cut(s) 111
ScrFI CCNGG 2 cut(s) 188, 366
SduI GDGCHC 1 cut(s) 252
SfcI CTRYAG 1 cut(s) 525
SinI GGWCC 1 cut(s) 382
SmlI CTYRAG 1 cut(s) 126
SmoI CTYRAG 1 cut(s) 126
Sse9I AATT 4 cut(s) 78, 284, 428, 497
SsiI CCGC 3 cut(s) 142, 196, 513
SspMI CTAG 1 cut(s) 270
StyD4I CCNGG 2 cut(s) 186, 364
TaaI ACNGT 2 cut(s) 87, 96
TaqI TCGA 6 cut(s) 115, 234, 240, 246, 391, 478
TasI AATT 4 cut(s) 78, 284, 428, 497
TauI GCSGC 1 cut(s) 516
TfiI GAWTC 2 cut(s) 275, 605
Tru1I TTAA 4 cut(s) 12, 230, 287, 637
Tru9I TTAA 4 cut(s) 12, 230, 287, 637
TseI GCWGC 1 cut(s) 624
TspDTI ATGAA 1 cut(s) 493
TspGWI ACGGA 2 cut(s) 390, 563
VpaK11BI GGWCC 1 cut(s) 382
VspI ATTAAT 1 cut(s) 287
XcmI CCANNNNNNNNNTGG 1 cut(s) 106
XspI CTAG 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.