pycom16g20880

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0002459
Physical Location & Seq
Forward (+)
18965032 .. 18966175
1144 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g20880.2

Sequence Viewer

Length: 537 bp
ATGGCCCGACTCCTCACTCAAACTCTCCATCTCCGTCAATCGCTCCTTCGCCGCCCGCTCCTCCCACCCTCCCTCACCCAAGCCCACCGCGCCCGCTCCTCCCGATCCGGCAAGGCCGAGTTAATCGAGATCGAGCTCGACCCCTCCACATCGTCGTCGCCAAGTGATTCAGAGAAATTAATGCTCAAGAAACTGGACGACATCGTTCAGACCATCGTCGTCCAGAGGGCCACGCCCGATTGGCTCCCCTTTGTCCCCGGCTCCTCGTTCTGGGTCCCACCTCGACGCGCCCCGTTGAAAGTGACCGACTTGGTTGGCAAATTGGCTGACCAGCTCACGGATGAAGAAACCCTCTCTGTGGCCACTGATCGTGGATGGCCTTGCTCCCAATTTTTCATCAATGGCGGTACTGGATCTGCAGAAACAAGGGAAGTGGATACGGAGGCAGAAGGATCAACAGAAATAGAGGTGGAGGTGGAGGTGGAGGTAAAGGTTTTGACTGATTCACAGAAGCCCTCTCGCTGTGAGGATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

179

Amino Acids

19.71

Weight (kDa)

4.9

Isoelectric Point (pI)

57.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 58, 96
AccII CGCG 2 cut(s) 90, 288
AciI CCGC 5 cut(s) 52, 56, 88, 94, 405
AclWI GGATC 3 cut(s) 99, 421, 460
AcoI YGGCCR 1 cut(s) 360
AfaI GTAC 1 cut(s) 409
AfiI CCNNNNNNNGG 3 cut(s) 270, 337, 358
AgsI TTSAA 1 cut(s) 298
AluBI AGCT 2 cut(s) 136, 334
AluI AGCT 2 cut(s) 136, 334
Alw21I GWGCWC 1 cut(s) 138
AlwI GGATC 3 cut(s) 99, 421, 460
AoxI GGCC 5 cut(s) 3, 114, 228, 360, 377
AseI ATTAAT 1 cut(s) 179
AspLEI GCGC 2 cut(s) 92, 290
AspS9I GGNCC 3 cut(s) 4, 228, 274
AsuC2I CCSGG 1 cut(s) 258
AsuHPI GGTGA 1 cut(s) 67
AvaII GGWCC 1 cut(s) 274
BalI TGGCCA 1 cut(s) 362
BanII GRGCYC 1 cut(s) 138
Bbv12I GWGCWC 1 cut(s) 138
BccI CCATC 3 cut(s) 36, 221, 369
BciVI GTATCC 1 cut(s) 430
BcnI CCSGG 1 cut(s) 258
BfmI CTRYAG 1 cut(s) 417
BfuI GTATCC 1 cut(s) 430
BglI GCCNNNNNGGC 1 cut(s) 241
BisI GCNGC 1 cut(s) 52
BlsI GCNGC 1 cut(s) 53
Bme1390I CCNGG 1 cut(s) 258
Bme18I GGWCC 1 cut(s) 274
BmgT120I GGNCC 3 cut(s) 4, 228, 274
BmiI GGNNCC 4 cut(s) 245, 262, 275, 276
BmrFI CCNGG 1 cut(s) 258
BoxI GACNNNNGTC 1 cut(s) 215
BpuEI CTTGAG 1 cut(s) 170
BpuMI CCSGG 1 cut(s) 258
BsaJI CCNNGG 1 cut(s) 256
Bsc4I CCNNNNNNNGG 3 cut(s) 270, 337, 358
Bse1I ACTGG 2 cut(s) 198, 415
BseDI CCNNGG 1 cut(s) 256
BseGI GGATG 3 cut(s) 346, 380, 535
BseLI CCNNNNNNNGG 3 cut(s) 270, 337, 358
BseNI ACTGG 2 cut(s) 198, 415
BseRI GAGGAG 3 cut(s) 50, 88, 253
Bsh1236I CGCG 2 cut(s) 90, 288
BshFI GGCC 5 cut(s) 5, 116, 230, 362, 379
BsiHKAI GWGCWC 1 cut(s) 138
BsiSI CCGG 2 cut(s) 108, 258
BslFI GGGAC 2 cut(s) 239, 260
BslI CCNNNNNNNGG 3 cut(s) 270, 337, 358
BsmFI GGGAC 2 cut(s) 239, 260
BsnI GGCC 5 cut(s) 5, 116, 230, 362, 379
Bsp1286I GDGCHC 1 cut(s) 138
Bsp143I GATC 5 cut(s) 104, 129, 367, 413, 452
BspACI CCGC 5 cut(s) 52, 56, 88, 94, 405
BspANI GGCC 5 cut(s) 5, 116, 230, 362, 379
BspFNI CGCG 2 cut(s) 90, 288
BspLI GGNNCC 4 cut(s) 245, 262, 275, 276
BspMAI CTGCAG 1 cut(s) 421
BspPI GGATC 3 cut(s) 99, 421, 460
BsrBI CCGCTC 2 cut(s) 58, 96
BsrI ACTGG 2 cut(s) 198, 415
BssECI CCNNGG 1 cut(s) 256
BssMI GATC 5 cut(s) 104, 129, 367, 413, 452
BstC8I GCNNGC 2 cut(s) 56, 94
BstF5I GGATG 3 cut(s) 346, 380, 535
BstFNI CGCG 2 cut(s) 90, 288
BstHHI GCGC 2 cut(s) 92, 290
BstKTI GATC 5 cut(s) 107, 132, 370, 416, 455
BstMBI GATC 5 cut(s) 104, 129, 367, 413, 452
BstMWI GCNNNNNNNGC 2 cut(s) 89, 241
BstPAI GACNNNNGTC 1 cut(s) 215
BstSCI CCNGG 1 cut(s) 256
BstSFI CTRYAG 1 cut(s) 417
BstUI CGCG 2 cut(s) 90, 288
BstX2I RGATCY 1 cut(s) 413
BstYI RGATCY 1 cut(s) 413
BsuI GTATCC 1 cut(s) 430
BsuRI GGCC 5 cut(s) 5, 116, 230, 362, 379
BtsCI GGATG 3 cut(s) 346, 380, 535
BtsIMutI CAGTG 1 cut(s) 363
Cac8I GCNNGC 2 cut(s) 56, 94
CfoI GCGC 2 cut(s) 92, 290
Cfr13I GGNCC 3 cut(s) 4, 228, 274
CseI GACGC 1 cut(s) 294
Csp6I GTAC 1 cut(s) 408
CspCI CAANNNNNGTGG 2 cut(s) 414, 449
CviQI GTAC 1 cut(s) 408
DpnI GATC 5 cut(s) 106, 131, 369, 415, 454
DpnII GATC 5 cut(s) 104, 129, 367, 413, 452
EaeI YGGCCR 1 cut(s) 360
Ecl136II GAGCTC 1 cut(s) 136
Eco24I GRGCYC 1 cut(s) 138
Eco47I GGWCC 1 cut(s) 274
Eco53kI GAGCTC 1 cut(s) 136
EcoICRI GAGCTC 1 cut(s) 136
EcoO109I RGGNCCY 1 cut(s) 274
EcoT38I GRGCYC 1 cut(s) 138
FaqI GGGAC 2 cut(s) 239, 260
FauI CCCGC 2 cut(s) 63, 101
Fnu4HI GCNGC 1 cut(s) 52
FokI GGATG 2 cut(s) 353, 387
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 1 cut(s) 52
GlaI GCGC 2 cut(s) 91, 289
GluI GCNGC 1 cut(s) 52
HaeIII GGCC 5 cut(s) 5, 116, 230, 362, 379
HapII CCGG 2 cut(s) 108, 258
HgaI GACGC 1 cut(s) 294
HhaI GCGC 2 cut(s) 92, 290
Hin6I GCGC 2 cut(s) 90, 288
HinP1I GCGC 2 cut(s) 90, 288
HinfI GANTC 3 cut(s) 9, 167, 503
HpaII CCGG 2 cut(s) 108, 258
HphI GGTGA 1 cut(s) 67
Hpy188I TCNGA 2 cut(s) 172, 210
Hpy188III TCNNGA 4 cut(s) 102, 127, 187, 223
Hpy99I CGWCG 4 cut(s) 157, 160, 221, 288
HpyAV CCTTC 2 cut(s) 56, 443
HpyCH4V TGCA 1 cut(s) 419
HpyF10VI GCNNNNNNNGC 2 cut(s) 89, 241
HspAI GCGC 2 cut(s) 90, 288
KflI GGGWCCC 1 cut(s) 274
Kzo9I GATC 5 cut(s) 104, 129, 367, 413, 452
LmnI GCTCC 6 cut(s) 48, 63, 101, 249, 266, 389
LpnPI CCDG 7 cut(s) 121, 179, 236, 256, 271, 344, 396
MaeIII GTNAC 1 cut(s) 301
MalI GATC 5 cut(s) 106, 131, 369, 415, 454
MbiI CCGCTC 2 cut(s) 58, 96
MboI GATC 5 cut(s) 104, 129, 367, 413, 452
MboII GAAGA 1 cut(s) 356
MflI RGATCY 1 cut(s) 413
MhlI GDGCHC 1 cut(s) 138
MlsI TGGCCA 1 cut(s) 362
MluCI AATT 3 cut(s) 176, 320, 389
MluNI TGGCCA 1 cut(s) 362
MlyI GAGTC 1 cut(s) 3
Mox20I TGGCCA 1 cut(s) 362
MscI TGGCCA 1 cut(s) 362
MseI TTAA 3 cut(s) 122, 179, 535
Msp20I TGGCCA 1 cut(s) 362
MspI CCGG 2 cut(s) 108, 258
MspR9I CCNGG 1 cut(s) 258
MvnI CGCG 2 cut(s) 90, 288
MwoI GCNNNNNNNGC 2 cut(s) 89, 241
NciI CCSGG 1 cut(s) 258
NdeII GATC 5 cut(s) 104, 129, 367, 413, 452
NlaIV GGNNCC 4 cut(s) 245, 262, 275, 276
NmeAIII GCCGAG 1 cut(s) 142
NmuCI GTSAC 1 cut(s) 301
PfeI GAWTC 2 cut(s) 167, 503
PkrI GCNGC 1 cut(s) 53
PleI GAGTC 1 cut(s) 3
PpsI GAGTC 1 cut(s) 3
PpuMI RGGWCCY 1 cut(s) 274
PshAI GACNNNNGTC 1 cut(s) 215
PshBI ATTAAT 1 cut(s) 179
Psp124BI GAGCTC 1 cut(s) 138
Psp5II RGGWCCY 1 cut(s) 274
PspN4I GGNNCC 4 cut(s) 245, 262, 275, 276
PspPI GGNCC 3 cut(s) 4, 228, 274
PspPPI RGGWCCY 1 cut(s) 274
PstI CTGCAG 1 cut(s) 421
PsuI RGATCY 1 cut(s) 413
RsaI GTAC 1 cut(s) 409
RsaNI GTAC 1 cut(s) 408
SacI GAGCTC 1 cut(s) 138
SaqAI TTAA 3 cut(s) 122, 179, 535
SatI GCNGC 1 cut(s) 52
Sau3AI GATC 5 cut(s) 104, 129, 367, 413, 452
Sau96I GGNCC 3 cut(s) 4, 228, 274
SchI GAGTC 1 cut(s) 3
ScrFI CCNGG 1 cut(s) 258
SduI GDGCHC 1 cut(s) 138
SetI ASST 8 cut(s) 138, 283, 336, 471, 477, 483, 489, 495
SfcI CTRYAG 1 cut(s) 417
SinI GGWCC 1 cut(s) 274
SmlI CTYRAG 1 cut(s) 185
SmoI CTYRAG 1 cut(s) 185
Sse9I AATT 3 cut(s) 176, 320, 389
SsiI CCGC 5 cut(s) 52, 56, 88, 94, 405
SstI GAGCTC 1 cut(s) 138
StyD4I CCNGG 1 cut(s) 256
TaqI TCGA 4 cut(s) 126, 132, 138, 283
TaqII GACCGA 1 cut(s) 320
TasI AATT 3 cut(s) 176, 320, 389
TauI GCSGC 1 cut(s) 54
TfiI GAWTC 2 cut(s) 167, 503
Tru1I TTAA 3 cut(s) 122, 179, 535
Tru9I TTAA 3 cut(s) 122, 179, 535
TscAI CASTG 1 cut(s) 370
TseFI GTSAC 1 cut(s) 301
Tsp45I GTSAC 1 cut(s) 301
TspDTI ATGAA 2 cut(s) 357, 385
TspGWI ACGGA 3 cut(s) 23, 353, 455
TspRI CASTG 1 cut(s) 370
VpaK11BI GGWCC 1 cut(s) 274
VspI ATTAAT 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.