MD17G1266300.v1.1

helicase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002485
Physical Location & Seq
Reverse (-)
32680875 .. 32682770
1896 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1266300.v1.1.491

Sequence Viewer

Length: 1344 bp
ATGAGAGTGCAACCGCGATTACCCGTTTCCTCAGTTAGTAGACTGCGAGCTAAATCTTCCTATGCCAGTGCTTACAGAGAAGCTAAAAATGAGCATTCTGGGGGTGATGCTTGTGAACAAAGATTGACAAATGGACATCAGGAAGCTGGGATGCCTGAAGATAGGAATGATTTGCAATGTGAATCCAACCAGATCAAAATAAGCGAATGTCAGAAATTAGATGGGTCACCTGTATATAGGCATCAGCAAGGTAGTAACATATCACCCTACCGAAAGGAATGTTTTCATCATGAAGAAAAAGGTTTTCTTGGTCTTCCTGAAAAAGCGAAGAATTCCAGAGATACTAGTAGCTCTGGAAAGTATCGAAAAGACCACAACAATTTTAGGGAATTATTGGCTACTGAGAATGTTGCTCAATGGGAAATGGGCCCAGGGAGTCCTGTATTTGAGAAGAATCTGTATATTGATTTTGTGCATACTGTAAAATCTCCTAAGTCGAAGTCACATTCCTCAGATACAAAGGGCCATATTATTCAGTACAGAGGGAATGATATTGAAATTCCTGAGAAAAGAAATGAAGTGAAAGAAACTCCTTCGGCAGAGTTTTCATTCCAAGATATTGAGTGCTTGGGTGATGGAAATGAAAAGACAATAGTGCCATTTCAAAGTTTGGAGTTTCCTGACTCCAGCTTTCTATCTTGGTCTGGCAGATCCAAGAAGGATGATCAAACAGACATGAGAAATGGTTTTTTATCAGGTCAGGATTCTAGCAAATTTGCAAGCTCAAAAGTGGCTGAACAAGAAAGGTGTAACTTGAAAAGCCAACATTTAGAGAAATCAGTTCATTGGGAAAATTTTAATGGCCTTACTCAGGATTGTATTACATCGACAAGGGCGAAACTGGATGATGAGAAAAAGAGTGATTTAAAAAGCCAACAGTCTAGAAAATCAGGAACAGAAGAAAGTTCTCATAGCCATGTTCAGAAGTCTATTACATTGACCAGCTCAAAAGTGGCCAACGGAGGAAAGATTGATTTAGAAAGTCAACAACTTGTGAAATTTGGTAATCAAGAAAGTTCCCAGGGCCACAATTCACGACGGCCTCTTGGACCACCTTTACCGAAATCTCCATCAGAGTCTTGGCTAAAGCGTACCTTACCTAGTATTTCCTCGAGGAGCTCATCTTCACAGTGTTCTTCTGGTTCACAGATTTATGCTATCAGCCAGCCTTCGAAGACGTCCTCTCTTGGTACCAAATGGGAGACAATTGTTAAAACTTCTAACAAGCACCATGGACATCTGCGGTTTTCAGAGGAGCTTCTGACACCTATACCGGAAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000280 GO:0000900 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006417 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007140 GO:0007275 GO:0007276 GO:0007281 GO:0007282 GO:0007283 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008356 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0016043 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017145 GO:0017148 GO:0019222 GO:0019827 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0030154 GO:0030371 GO:0030718 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032504 GO:0034248 GO:0034249 GO:0034641 GO:0035212 GO:0040008 GO:0042078 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044703 GO:0045182 GO:0046483 GO:0048232 GO:0048285 GO:0048468 GO:0048519 GO:0048523 GO:0048609 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051301 GO:0051321 GO:0051704 GO:0060255 GO:0065007 GO:0070013 GO:0070035 GO:0071704 GO:0071840 GO:0080090 GO:0090079 GO:0090304 GO:0097159 GO:0098724 GO:0098725 GO:0098727 GO:0098729 GO:0098730 GO:0140013 GO:0140098 GO:1901360 GO:1901363 GO:1903046 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind Pkinase R3H

Protein Analysis

448

Amino Acids

50.03

Weight (kDa)

8.6

Isoelectric Point (pI)

59.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1241
AbsI CCTCGAGG 1 cut(s) 1171
Acc65I GGTACC 1 cut(s) 1250
AccB1I GGYRCC 1 cut(s) 1250
AccI GTMKAC 1 cut(s) 40
AccII CGCG 1 cut(s) 16
AciI CCGC 2 cut(s) 14, 1303
AclWI GGATC 1 cut(s) 705
AcoI YGGCCR 1 cut(s) 1014
AcsI RAATTY 5 cut(s) 331, 558, 773, 853, 1058
AcuI CTGAAG 1 cut(s) 177
AcyI GRCGYC 1 cut(s) 1238
AfaI GTAC 3 cut(s) 539, 1153, 1252
AfiI CCNNNNNNNGG 1 cut(s) 871
AgsI TTSAA 3 cut(s) 557, 665, 817
AhlI ACTAGT 1 cut(s) 344
AjnI CCWGG 2 cut(s) 430, 1080
Alw21I GWGCWC 1 cut(s) 1181
Alw26I GTCTC 1 cut(s) 1256
AlwI GGATC 1 cut(s) 705
Ama87I CYCGRG 1 cut(s) 1171
AoxI GGCC 6 cut(s) 427, 523, 862, 1014, 1084, 1100
ApaI GGGCCC 1 cut(s) 431
ApoI RAATTY 5 cut(s) 331, 558, 773, 853, 1058
Asp700I GAANNNNTTC 1 cut(s) 282
Asp718I GGTACC 1 cut(s) 1250
AspS9I GGNCC 5 cut(s) 427, 428, 523, 1084, 1109
AsuHPI GGTGA 4 cut(s) 116, 219, 255, 644
AsuII TTCGAA 1 cut(s) 1232
AvaI CYCGRG 1 cut(s) 1171
AvaII GGWCC 1 cut(s) 1109
BaeGI GKGCMC 1 cut(s) 431
BalI TGGCCA 1 cut(s) 1016
BanI GGYRCC 1 cut(s) 1250
BanII GRGCYC 2 cut(s) 431, 1181
BbsI GAAGAC 2 cut(s) 305, 1241
Bbv12I GWGCWC 1 cut(s) 1181
BccI CCATC 3 cut(s) 215, 629, 1138
BceAI ACGGC 1 cut(s) 1115
BciT130I CCWGG 2 cut(s) 432, 1082
BclI TGATCA 1 cut(s) 724
BcoDI GTCTC 1 cut(s) 1256
BcuI ACTAGT 1 cut(s) 344
BfaI CTAG 4 cut(s) 345, 768, 942, 1161
Bme1390I CCNGG 2 cut(s) 432, 1082
Bme18I GGWCC 1 cut(s) 1109
BmeT110I CYCGRG 1 cut(s) 1171
BmgT120I GGNCC 5 cut(s) 427, 428, 523, 1084, 1109
BmiI GGNNCC 2 cut(s) 429, 1252
BmrFI CCNGG 2 cut(s) 432, 1082
BmsI GCATC 3 cut(s) 97, 141, 250
BpiI GAAGAC 2 cut(s) 305, 1241
BpmI CTGGAG 1 cut(s) 670
Bpu14I TTCGAA 1 cut(s) 1232
BsaHI GRCGYC 1 cut(s) 1238
BsaJI CCNNGG 5 cut(s) 430, 431, 1080, 1081, 1291
BsaWI WCCGGW 1 cut(s) 1333
Bsc4I CCNNNNNNNGG 1 cut(s) 871
Bse1I ACTGG 2 cut(s) 66, 906
Bse3DI GCAATG 1 cut(s) 182
BseBI CCWGG 2 cut(s) 432, 1082
BseDI CCNNGG 5 cut(s) 430, 431, 1080, 1081, 1291
BseGI GGATG 3 cut(s) 156, 727, 910
BseLI CCNNNNNNNGG 1 cut(s) 871
BseMI GCAATG 1 cut(s) 182
BseMII CTCAG 5 cut(s) 45, 393, 525, 555, 884
BseNI ACTGG 2 cut(s) 66, 906
BseRI GAGGAG 2 cut(s) 1189, 1328
BseSI GKGCMC 1 cut(s) 431
BseYI CCCAGC 1 cut(s) 146
Bsh1236I CGCG 1 cut(s) 16
BshFI GGCC 6 cut(s) 429, 525, 864, 1016, 1086, 1102
BshNI GGYRCC 1 cut(s) 1250
BsiHKAI GWGCWC 1 cut(s) 1181
BsiHKCI CYCGRG 1 cut(s) 1171
BsiSI CCGG 1 cut(s) 1334
BslI CCNNNNNNNGG 1 cut(s) 871
BsmAI GTCTC 1 cut(s) 1256
BsmI GAATGC 1 cut(s) 94
BsnI GGCC 6 cut(s) 429, 525, 864, 1016, 1086, 1102
BsoBI CYCGRG 1 cut(s) 1171
Bsp119I TTCGAA 1 cut(s) 1232
Bsp120I GGGCCC 1 cut(s) 427
Bsp1286I GDGCHC 2 cut(s) 431, 1181
Bsp143I GATC 3 cut(s) 192, 710, 724
Bsp19I CCATGG 1 cut(s) 1291
BspACI CCGC 2 cut(s) 14, 1303
BspANI GGCC 6 cut(s) 429, 525, 864, 1016, 1086, 1102
BspCNI CTCAG 5 cut(s) 44, 394, 524, 556, 883
BspFNI CGCG 1 cut(s) 16
BspHI TCATGA 1 cut(s) 289
BspLI GGNNCC 2 cut(s) 429, 1252
BspPI GGATC 1 cut(s) 705
BspT104I TTCGAA 1 cut(s) 1232
BspT107I GGYRCC 1 cut(s) 1250
BsrDI GCAATG 1 cut(s) 182
BsrI ACTGG 2 cut(s) 66, 906
BssECI CCNNGG 5 cut(s) 430, 431, 1080, 1081, 1291
BssMI GATC 3 cut(s) 192, 710, 724
BssNI GRCGYC 1 cut(s) 1238
BssT1I CCWWGG 1 cut(s) 1291
Bst2UI CCWGG 2 cut(s) 432, 1082
Bst4CI ACNGT 3 cut(s) 481, 939, 1191
BstACI GRCGYC 1 cut(s) 1238
BstBI TTCGAA 1 cut(s) 1232
BstC8I GCNNGC 3 cut(s) 48, 781, 1226
BstDEI CTNAG 7 cut(s) 31, 402, 492, 511, 564, 870, 1341
BstDSI CCRYGG 1 cut(s) 1291
BstEII GGTNACC 1 cut(s) 225
BstENI CCTNNNNNAGG 1 cut(s) 869
BstF5I GGATG 3 cut(s) 156, 727, 910
BstFNI CGCG 1 cut(s) 16
BstKTI GATC 3 cut(s) 195, 713, 727
BstMAI GTCTC 1 cut(s) 1256
BstMBI GATC 3 cut(s) 192, 710, 724
BstNI CCWGG 2 cut(s) 432, 1082
BstPI GGTNACC 1 cut(s) 225
BstSCI CCNGG 2 cut(s) 430, 1080
BstSLI GKGCMC 1 cut(s) 431
BstUI CGCG 1 cut(s) 16
BstV2I GAAGAC 2 cut(s) 305, 1241
BstX2I RGATCY 1 cut(s) 710
BstYI RGATCY 1 cut(s) 710
BsuRI GGCC 6 cut(s) 429, 525, 864, 1016, 1086, 1102
BtgI CCRYGG 1 cut(s) 1291
BtsCI GGATG 3 cut(s) 156, 727, 910
BtsIMutI CAGTG 2 cut(s) 73, 1196
Cac8I GCNNGC 3 cut(s) 48, 781, 1226
CciI TCATGA 1 cut(s) 289
Cfr13I GGNCC 5 cut(s) 427, 428, 523, 1084, 1109
Csp6I GTAC 3 cut(s) 538, 1152, 1251
CviAII CATG 4 cut(s) 290, 736, 977, 1292
CviQI GTAC 3 cut(s) 538, 1152, 1251
DdeI CTNAG 7 cut(s) 31, 402, 492, 511, 564, 870, 1341
DpnI GATC 3 cut(s) 194, 712, 726
DpnII GATC 3 cut(s) 192, 710, 724
DraI TTTAAA 1 cut(s) 927
EaeI YGGCCR 1 cut(s) 1014
Ecl136II GAGCTC 1 cut(s) 1179
Eco130I CCWWGG 1 cut(s) 1291
Eco24I GRGCYC 2 cut(s) 431, 1181
Eco47I GGWCC 1 cut(s) 1109
Eco53kI GAGCTC 1 cut(s) 1179
Eco57I CTGAAG 1 cut(s) 177
Eco88I CYCGRG 1 cut(s) 1171
Eco91I GGTNACC 1 cut(s) 225
EcoICRI GAGCTC 1 cut(s) 1179
EcoNI CCTNNNNNAGG 1 cut(s) 869
EcoO65I GGTNACC 1 cut(s) 225
EcoRI GAATTC 1 cut(s) 331
EcoRII CCWGG 2 cut(s) 430, 1080
EcoT14I CCWWGG 1 cut(s) 1291
EcoT38I GRGCYC 2 cut(s) 431, 1181
ErhI CCWWGG 1 cut(s) 1291
FaeI CATG 4 cut(s) 293, 739, 980, 1295
FalI AAGNNNNNCTT 4 cut(s) 291, 323, 1139, 1171
FatI CATG 4 cut(s) 289, 735, 976, 1291
FbaI TGATCA 1 cut(s) 724
FblI GTMKAC 1 cut(s) 40
FokI GGATG 3 cut(s) 163, 734, 917
FriOI GRGCYC 2 cut(s) 431, 1181
FspBI CTAG 4 cut(s) 345, 768, 942, 1161
GsaI CCCAGC 1 cut(s) 150
GsuI CTGGAG 1 cut(s) 670
HaeIII GGCC 6 cut(s) 429, 525, 864, 1016, 1086, 1102
HapII CCGG 1 cut(s) 1334
Hin1I GRCGYC 1 cut(s) 1238
Hin1II CATG 4 cut(s) 293, 739, 980, 1295
HincII GTYRAC 1 cut(s) 1046
HindII GTYRAC 1 cut(s) 1046
HindIII AAGCTT 1 cut(s) 1338
HinfI GANTC 6 cut(s) 182, 436, 454, 683, 764, 1136
HpaII CCGG 1 cut(s) 1334
HphI GGTGA 4 cut(s) 116, 219, 255, 644
Hpy166II GTNNAC 4 cut(s) 41, 116, 1046, 1205
Hpy188I TCNGA 6 cut(s) 213, 514, 984, 1135, 1312, 1323
Hpy8I GTNNAC 4 cut(s) 41, 116, 1046, 1205
Hpy99I CGWCG 1 cut(s) 1101
HpyAV CCTTC 3 cut(s) 603, 712, 1239
HpyCH4III ACNGT 3 cut(s) 481, 939, 1191
HpyCH4IV ACGT 1 cut(s) 1238
HpyCH4V TGCA 4 cut(s) 10, 175, 475, 779
HpyF3I CTNAG 7 cut(s) 31, 402, 492, 511, 564, 870, 1341
HpySE526I ACGT 1 cut(s) 1238
Hsp92I GRCGYC 1 cut(s) 1238
Hsp92II CATG 4 cut(s) 293, 739, 980, 1295
KpnI GGTACC 1 cut(s) 1254
Ksp22I TGATCA 1 cut(s) 724
Kzo9I GATC 3 cut(s) 192, 710, 724
LmnI GCTCC 2 cut(s) 1176, 1315
LweI GCATC 3 cut(s) 97, 141, 250
MaeI CTAG 4 cut(s) 345, 768, 942, 1161
MaeII ACGT 1 cut(s) 1238
MaeIII GTNAC 4 cut(s) 225, 254, 501, 809
MalI GATC 3 cut(s) 194, 712, 726
MboI GATC 3 cut(s) 192, 710, 724
MfeI CAATTG 1 cut(s) 1266
MflI RGATCY 1 cut(s) 710
MhlI GDGCHC 2 cut(s) 431, 1181
MlsI TGGCCA 1 cut(s) 1016
MluNI TGGCCA 1 cut(s) 1016
MlyI GAGTC 3 cut(s) 445, 677, 1145
MmeI TCCRAC 1 cut(s) 210
MnlI CCTC 9 cut(s) 40, 520, 536, 1016, 1113, 1167, 1180, 1252, 1306
Mox20I TGGCCA 1 cut(s) 1016
MroXI GAANNNNTTC 1 cut(s) 282
MscI TGGCCA 1 cut(s) 1016
MseI TTAA 3 cut(s) 858, 926, 1272
MslI CAYNNNNRTG 1 cut(s) 975
Msp20I TGGCCA 1 cut(s) 1016
MspI CCGG 1 cut(s) 1334
MspR9I CCNGG 2 cut(s) 432, 1082
MunI CAATTG 1 cut(s) 1266
Mva1269I GAATGC 1 cut(s) 94
MvaI CCWGG 2 cut(s) 432, 1082
MvnI CGCG 1 cut(s) 16
NcoI CCATGG 1 cut(s) 1291
NdeII GATC 3 cut(s) 192, 710, 724
NlaIII CATG 4 cut(s) 293, 739, 980, 1295
NlaIV GGNNCC 2 cut(s) 429, 1252
NmuCI GTSAC 2 cut(s) 225, 501
NspV TTCGAA 1 cut(s) 1232
PaeR7I CTCGAG 1 cut(s) 1171
PagI TCATGA 1 cut(s) 289
PasI CCCWGGG 2 cut(s) 431, 1081
PcsI WCGNNNNNNNCGW 1 cut(s) 893
PctI GAATGC 1 cut(s) 94
PdmI GAANNNNTTC 1 cut(s) 282
PfeI GAWTC 3 cut(s) 182, 454, 764
PleI GAGTC 3 cut(s) 444, 677, 1144
PpsI GAGTC 3 cut(s) 444, 677, 1144
Psp124BI GAGCTC 1 cut(s) 1181
Psp6I CCWGG 2 cut(s) 430, 1080
PspEI GGTNACC 1 cut(s) 225
PspFI CCCAGC 1 cut(s) 146
PspGI CCWGG 2 cut(s) 430, 1080
PspN4I GGNNCC 2 cut(s) 429, 1252
PspOMI GGGCCC 1 cut(s) 427
PspPI GGNCC 5 cut(s) 427, 428, 523, 1084, 1109
PspXI VCTCGAGB 1 cut(s) 1171
PsuI RGATCY 1 cut(s) 710
RsaI GTAC 3 cut(s) 539, 1153, 1252
RsaNI GTAC 3 cut(s) 538, 1152, 1251
RseI CAYNNNNRTG 1 cut(s) 975
SacI GAGCTC 1 cut(s) 1181
SaqAI TTAA 3 cut(s) 858, 926, 1272
Sau3AI GATC 3 cut(s) 192, 710, 724
Sau96I GGNCC 5 cut(s) 427, 428, 523, 1084, 1109
SchI GAGTC 3 cut(s) 445, 677, 1145
ScrFI CCNGG 2 cut(s) 432, 1082
SduI GDGCHC 2 cut(s) 431, 1181
SfaNI GCATC 3 cut(s) 97, 141, 250
Sfr274I CTCGAG 1 cut(s) 1171
SfuI TTCGAA 1 cut(s) 1232
SinI GGWCC 1 cut(s) 1109
SlaI CTCGAG 1 cut(s) 1171
SmiMI CAYNNNNRTG 1 cut(s) 975
SmlI CTYRAG 1 cut(s) 1171
SmoI CTYRAG 1 cut(s) 1171
SpeI ACTAGT 1 cut(s) 344
SsiI CCGC 2 cut(s) 14, 1303
SspMI CTAG 4 cut(s) 345, 768, 942, 1161
SstI GAGCTC 1 cut(s) 1181
StyD4I CCNGG 2 cut(s) 430, 1080
StyI CCWWGG 1 cut(s) 1291
TaaI ACNGT 3 cut(s) 481, 939, 1191
TaiI ACGT 1 cut(s) 1241
TaqI TCGA 5 cut(s) 364, 497, 887, 1172, 1232
TatI WGTACW 1 cut(s) 537
TfiI GAWTC 3 cut(s) 182, 454, 764
Tru1I TTAA 3 cut(s) 858, 926, 1272
Tru9I TTAA 3 cut(s) 858, 926, 1272
TscAI CASTG 2 cut(s) 73, 1196
TseFI GTSAC 2 cut(s) 225, 501
Tsp45I GTSAC 2 cut(s) 225, 501
TspDTI ATGAA 6 cut(s) 275, 306, 591, 597, 657, 833
TspGWI ACGGA 1 cut(s) 1035
TspRI CASTG 2 cut(s) 73, 1196
VpaK11BI GGWCC 1 cut(s) 1109
XagI CCTNNNNNAGG 1 cut(s) 869
XapI RAATTY 5 cut(s) 331, 558, 773, 853, 1058
XbaI TCTAGA 1 cut(s) 941
XcmI CCANNNNNNNNNTGG 2 cut(s) 1009, 1137
XhoI CTCGAG 1 cut(s) 1171
XmiI GTMKAC 1 cut(s) 40
XmnI GAANNNNTTC 1 cut(s) 282
XspI CTAG 4 cut(s) 345, 768, 942, 1161
ZraI GACGTC 1 cut(s) 1239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.