MD17G1266400.v1.1

helicase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002485
Physical Location & Seq
Reverse (-)
32682772 .. 32685359
2588 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1266400.v1.1.491

Sequence Viewer

Length: 879 bp
ATGCTAAAGAATATGATGGAAGAGAAGCAGTTAGATCTTAATCAGCCACTTCTGTCTGTGAGACGATTTTCACCTACAGCAGTCACCTTAGAAGCTAATGACAAAAGGAAGACTGATAAGTCAATACCCAAACTACCTCTTCCCTTTTACAAATCAGAACTGAAATCAGGTCCAGTGAGGCACCCAGGTAGTGTTCCTTTTGTATGGGAGAAAGTCCCGGGAAAACCCAAGGATGAAAGAAATTCACAGACTCGGGGTATTGAGCAGCCTCCCACTACGCCGAGGCTTCCGCCTGGAAGGGTTTTGAATGCTAAGAAGCAAGCATTGGATAAATGTTCTAAACGCACTACAACTGCTCAGTCCCCAACTGGAAATGTACTTTCCAATTTTAACAATGTTTCAACCTTTGATACAAAAGAAGTTACCAAGTGTGACAGCCTTAAAGAGGGGATGGATGATAAGGATATTGCTGATTCAGAGGATGGTGATGAGACCTATTTAGATGCACTTGACACACTTTCGAGGAGTGAATCATTCTATTATAACTGTAGTATAAGTGGTTTGAGTGGACTGGACGGTCCAGATGCCAAACCATCTGGAACCTTCTCAACAGATCCACAGACTCGCGACTTCATGATGGGTCGGTTCTTGCCTGCAGCAAAAGCAATGGTTTCAGACACACCTCAATATGCTACCAGGAAGCAACCACTTGCACGAGAGCAAGCAATTTCTCAAGAGCAACCTAGAGGGATGAAGAAGGTAGCAAGCGGGGTTAAGCAACATCCACTTAATCAATATAGGCCAAATGATTTGCCACATAATTTTCAAGTTATAGCTGGGGACAAAAGTGAGAATGAAGGTGATTATATAAGGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000280 GO:0000900 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006417 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007140 GO:0007275 GO:0007276 GO:0007281 GO:0007282 GO:0007283 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008356 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0016043 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017145 GO:0017148 GO:0019222 GO:0019827 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0030154 GO:0030371 GO:0030718 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032504 GO:0034248 GO:0034249 GO:0034641 GO:0035212 GO:0040008 GO:0042078 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044703 GO:0045182 GO:0046483 GO:0048232 GO:0048285 GO:0048468 GO:0048519 GO:0048523 GO:0048609 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051301 GO:0051321 GO:0051704 GO:0060255 GO:0065007 GO:0070013 GO:0070035 GO:0071704 GO:0071840 GO:0080090 GO:0090079 GO:0090304 GO:0097159 GO:0098724 GO:0098725 GO:0098727 GO:0098729 GO:0098730 GO:0140013 GO:0140098 GO:1901360 GO:1901363 GO:1903046 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind Pkinase R3H

Protein Analysis

293

Amino Acids

32.54

Weight (kDa)

8.78

Isoelectric Point (pI)

44.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF688 PF05097 6 - 284 1.6e-76 Protein of unknown function (DUF688)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 543
AasI GACNNNNNNGTC 2 cut(s) 118, 576
AccB1I GGYRCC 1 cut(s) 180
AccII CGCG 1 cut(s) 627
AciI CCGC 2 cut(s) 290, 768
AclWI GGATC 1 cut(s) 608
AcsI RAATTY 1 cut(s) 241
AfaI GTAC 1 cut(s) 378
AfiI CCNNNNNNNGG 1 cut(s) 445
AgsI TTSAA 3 cut(s) 307, 402, 827
AjnI CCWGG 3 cut(s) 184, 292, 695
AjuI GAANNNNNNNTTGG 2 cut(s) 308, 340
AluBI AGCT 2 cut(s) 95, 836
AluI AGCT 2 cut(s) 95, 836
Alw26I GTCTC 2 cut(s) 55, 485
AlwI GGATC 1 cut(s) 608
Ama87I CYCGRG 2 cut(s) 217, 252
AoxI GGCC 1 cut(s) 800
ApeKI GCWGC 2 cut(s) 265, 656
ApoI RAATTY 1 cut(s) 241
AspS9I GGNCC 2 cut(s) 170, 578
AsuC2I CCSGG 2 cut(s) 218, 219
AsuHPI GGTGA 4 cut(s) 63, 76, 497, 872
AvaI CYCGRG 2 cut(s) 217, 252
AvaII GGWCC 2 cut(s) 170, 578
BanI GGYRCC 1 cut(s) 180
BauI CACGAG 1 cut(s) 714
BbsI GAAGAC 1 cut(s) 116
BbvI GCAGC 2 cut(s) 277, 668
BccI CCATC 5 cut(s) 10, 445, 476, 601, 631
BciT130I CCWGG 3 cut(s) 186, 294, 697
BcnI CCSGG 2 cut(s) 218, 219
BcoDI GTCTC 2 cut(s) 55, 485
BfaI CTAG 1 cut(s) 744
BfmI CTRYAG 3 cut(s) 75, 547, 654
BglII AGATCT 1 cut(s) 34
BisI GCNGC 2 cut(s) 266, 657
BlsI GCNGC 2 cut(s) 267, 658
Bme1390I CCNGG 5 cut(s) 186, 218, 219, 294, 697
Bme18I GGWCC 2 cut(s) 170, 578
BmeT110I CYCGRG 2 cut(s) 217, 252
BmgT120I GGNCC 2 cut(s) 170, 578
BmiI GGNNCC 2 cut(s) 182, 601
BmrFI CCNGG 5 cut(s) 186, 218, 219, 294, 697
BmsI GCATC 2 cut(s) 493, 574
BpiI GAAGAC 1 cut(s) 116
BpuEI CTTGAG 1 cut(s) 717
BpuMI CCSGG 2 cut(s) 218, 219
BsaBI GATNNNNATC 1 cut(s) 39
BsaI GGTCTC 1 cut(s) 485
BsaJI CCNNGG 4 cut(s) 184, 217, 228, 281
Bsc4I CCNNNNNNNGG 1 cut(s) 445
Bse1I ACTGG 3 cut(s) 173, 373, 576
Bse3DI GCAATG 1 cut(s) 672
Bse8I GATNNNNATC 1 cut(s) 39
BseBI CCWGG 3 cut(s) 186, 294, 697
BseDI CCNNGG 4 cut(s) 184, 217, 228, 281
BseGI GGATG 6 cut(s) 238, 456, 460, 487, 756, 781
BseJI GATNNNNATC 1 cut(s) 39
BseLI CCNNNNNNNGG 1 cut(s) 445
BseMI GCAATG 1 cut(s) 672
BseMII CTCAG 1 cut(s) 371
BseNI ACTGG 3 cut(s) 173, 373, 576
BseRI GAGGAG 1 cut(s) 538
BseXI GCAGC 2 cut(s) 277, 668
BseYI CCCAGC 1 cut(s) 836
Bsh1236I CGCG 1 cut(s) 627
BshFI GGCC 1 cut(s) 802
BshNI GGYRCC 1 cut(s) 180
BsiHKCI CYCGRG 2 cut(s) 217, 252
BsiSI CCGG 1 cut(s) 218
BslFI GGGAC 3 cut(s) 200, 346, 854
BslI CCNNNNNNNGG 1 cut(s) 445
BsmAI GTCTC 2 cut(s) 55, 485
BsmBI CGTCTC 1 cut(s) 55
BsmFI GGGAC 3 cut(s) 200, 346, 854
BsmI GAATGC 1 cut(s) 313
BsnI GGCC 1 cut(s) 802
Bso31I GGTCTC 1 cut(s) 485
BsoBI CYCGRG 2 cut(s) 217, 252
Bsp143I GATC 3 cut(s) 34, 613, 873
Bsp68I TCGCGA 1 cut(s) 627
BspACI CCGC 2 cut(s) 290, 768
BspANI GGCC 1 cut(s) 802
BspCNI CTCAG 1 cut(s) 370
BspFNI CGCG 1 cut(s) 627
BspHI TCATGA 1 cut(s) 633
BspLI GGNNCC 2 cut(s) 182, 601
BspMAI CTGCAG 1 cut(s) 658
BspPI GGATC 1 cut(s) 608
BspT107I GGYRCC 1 cut(s) 180
BspTNI GGTCTC 1 cut(s) 485
BsrDI GCAATG 1 cut(s) 672
BsrI ACTGG 3 cut(s) 173, 373, 576
BssECI CCNNGG 4 cut(s) 184, 217, 228, 281
BssMI GATC 3 cut(s) 34, 613, 873
BssSI CACGAG 1 cut(s) 714
BssT1I CCWWGG 1 cut(s) 228
Bst2BI CACGAG 1 cut(s) 714
Bst2UI CCWGG 3 cut(s) 186, 294, 697
Bst4CI ACNGT 2 cut(s) 548, 578
Bst6I CTCTTC 2 cut(s) 15, 144
BstC8I GCNNGC 4 cut(s) 321, 654, 723, 766
BstDEI CTNAG 3 cut(s) 88, 312, 357
BstENI CCTNNNNNAGG 1 cut(s) 443
BstF5I GGATG 6 cut(s) 238, 456, 460, 487, 756, 781
BstFNI CGCG 1 cut(s) 627
BstKTI GATC 3 cut(s) 37, 616, 876
BstMAI GTCTC 2 cut(s) 55, 485
BstMBI GATC 3 cut(s) 34, 613, 873
BstMWI GCNNNNNNNGC 1 cut(s) 662
BstNI CCWGG 3 cut(s) 186, 294, 697
BstSCI CCNGG 5 cut(s) 184, 216, 217, 292, 695
BstSFI CTRYAG 3 cut(s) 75, 547, 654
BstUI CGCG 1 cut(s) 627
BstV1I GCAGC 2 cut(s) 277, 668
BstV2I GAAGAC 1 cut(s) 116
BstX2I RGATCY 3 cut(s) 34, 613, 873
BstYI RGATCY 3 cut(s) 34, 613, 873
BsuRI GGCC 1 cut(s) 802
BtsCI GGATG 6 cut(s) 238, 456, 460, 487, 756, 781
BtsIMutI CAGTG 1 cut(s) 180
BtuMI TCGCGA 1 cut(s) 627
Cac8I GCNNGC 4 cut(s) 321, 654, 723, 766
CciI TCATGA 1 cut(s) 633
Cfr13I GGNCC 2 cut(s) 170, 578
Cfr9I CCCGGG 1 cut(s) 217
Csp6I GTAC 1 cut(s) 377
CviAII CATG 1 cut(s) 634
CviJI RGCY 7 cut(s) 46, 95, 268, 286, 438, 802, 836
CviKI_1 RGCY 7 cut(s) 46, 95, 268, 286, 438, 802, 836
CviQI GTAC 1 cut(s) 377
DdeI CTNAG 3 cut(s) 88, 312, 357
DpnI GATC 3 cut(s) 36, 615, 875
DpnII GATC 3 cut(s) 34, 613, 873
DrdI GACNNNNNNGTC 2 cut(s) 118, 576
DseDI GACNNNNNNGTC 2 cut(s) 118, 576
Eam1104I CTCTTC 2 cut(s) 15, 144
EarI CTCTTC 2 cut(s) 15, 144
EciI GGCGGA 1 cut(s) 279
Eco130I CCWWGG 1 cut(s) 228
Eco31I GGTCTC 1 cut(s) 485
Eco47I GGWCC 2 cut(s) 170, 578
Eco88I CYCGRG 2 cut(s) 217, 252
EcoNI CCTNNNNNAGG 1 cut(s) 443
EcoRII CCWGG 3 cut(s) 184, 292, 695
EcoT14I CCWWGG 1 cut(s) 228
ErhI CCWWGG 1 cut(s) 228
Esp3I CGTCTC 1 cut(s) 55
FaeI CATG 1 cut(s) 637
FaqI GGGAC 3 cut(s) 200, 346, 854
FatI CATG 1 cut(s) 633
FauI CCCGC 1 cut(s) 761
Fnu4HI GCNGC 2 cut(s) 266, 657
FokI GGATG 6 cut(s) 245, 463, 467, 494, 763, 768
Fsp4HI GCNGC 2 cut(s) 266, 657
FspBI CTAG 1 cut(s) 744
GluI GCNGC 2 cut(s) 266, 657
GsaI CCCAGC 1 cut(s) 840
HaeIII GGCC 1 cut(s) 802
HapII CCGG 1 cut(s) 218
Hin1II CATG 1 cut(s) 637
HinfI GANTC 4 cut(s) 250, 473, 530, 622
HpaII CCGG 1 cut(s) 218
HphI GGTGA 4 cut(s) 63, 76, 497, 872
Hpy166II GTNNAC 1 cut(s) 569
Hpy188I TCNGA 4 cut(s) 157, 478, 676, 878
Hpy188III TCNNGA 5 cut(s) 581, 597, 626, 634, 734
Hpy8I GTNNAC 1 cut(s) 569
HpyAV CCTTC 4 cut(s) 291, 613, 751, 851
HpyCH4III ACNGT 2 cut(s) 548, 578
HpyCH4V TGCA 3 cut(s) 506, 656, 713
HpyF10VI GCNNNNNNNGC 1 cut(s) 662
HpyF3I CTNAG 3 cut(s) 88, 312, 357
Hsp92II CATG 1 cut(s) 637
Kzo9I GATC 3 cut(s) 34, 613, 873
Lsp1109I GCAGC 2 cut(s) 277, 668
LweI GCATC 2 cut(s) 493, 574
MaeI CTAG 1 cut(s) 744
MaeIII GTNAC 3 cut(s) 82, 421, 431
MalI GATC 3 cut(s) 36, 615, 875
MboI GATC 3 cut(s) 34, 613, 873
MboII GAAGA 4 cut(s) 32, 121, 131, 766
MflI RGATCY 3 cut(s) 34, 613, 873
MluCI AATT 4 cut(s) 241, 385, 726, 820
MlyI GAGTC 2 cut(s) 244, 616
MnlI CCTC 9 cut(s) 147, 171, 276, 279, 439, 472, 516, 693, 740
MseI TTAA 5 cut(s) 39, 390, 441, 774, 789
MspI CCGG 1 cut(s) 218
MspR9I CCNGG 5 cut(s) 186, 218, 219, 294, 697
Mva1269I GAATGC 1 cut(s) 313
MvaI CCWGG 3 cut(s) 186, 294, 697
MvnI CGCG 1 cut(s) 627
MwoI GCNNNNNNNGC 1 cut(s) 662
NciI CCSGG 2 cut(s) 218, 219
NdeII GATC 3 cut(s) 34, 613, 873
NlaIII CATG 1 cut(s) 637
NlaIV GGNNCC 2 cut(s) 182, 601
NmeAIII GCCGAG 1 cut(s) 306
NmuCI GTSAC 2 cut(s) 82, 431
NruI TCGCGA 1 cut(s) 627
PagI TCATGA 1 cut(s) 633
PctI GAATGC 1 cut(s) 313
PfeI GAWTC 2 cut(s) 473, 530
PkrI GCNGC 2 cut(s) 267, 658
PleI GAGTC 2 cut(s) 244, 616
PpsI GAGTC 2 cut(s) 244, 616
PsiI TTATAA 1 cut(s) 543
Psp6I CCWGG 3 cut(s) 184, 292, 695
PspFI CCCAGC 1 cut(s) 836
PspGI CCWGG 3 cut(s) 184, 292, 695
PspN4I GGNNCC 2 cut(s) 182, 601
PspPI GGNCC 2 cut(s) 170, 578
PstI CTGCAG 1 cut(s) 658
PsuI RGATCY 3 cut(s) 34, 613, 873
RruI TCGCGA 1 cut(s) 627
RsaI GTAC 1 cut(s) 378
RsaNI GTAC 1 cut(s) 377
SaqAI TTAA 5 cut(s) 39, 390, 441, 774, 789
SatI GCNGC 2 cut(s) 266, 657
Sau3AI GATC 3 cut(s) 34, 613, 873
Sau96I GGNCC 2 cut(s) 170, 578
SchI GAGTC 2 cut(s) 244, 616
ScrFI CCNGG 5 cut(s) 186, 218, 219, 294, 697
SfaNI GCATC 2 cut(s) 493, 574
SfcI CTRYAG 3 cut(s) 75, 547, 654
SinI GGWCC 2 cut(s) 170, 578
SmaI CCCGGG 1 cut(s) 219
SmlI CTYRAG 1 cut(s) 732
SmoI CTYRAG 1 cut(s) 732
Sse9I AATT 4 cut(s) 241, 385, 726, 820
SsiI CCGC 2 cut(s) 290, 768
SspMI CTAG 1 cut(s) 744
StyD4I CCNGG 5 cut(s) 184, 216, 217, 292, 695
StyI CCWWGG 1 cut(s) 228
TaaI ACNGT 2 cut(s) 548, 578
TaqI TCGA 1 cut(s) 521
TasI AATT 4 cut(s) 241, 385, 726, 820
TatI WGTACW 1 cut(s) 376
TfiI GAWTC 2 cut(s) 473, 530
Tru1I TTAA 5 cut(s) 39, 390, 441, 774, 789
Tru9I TTAA 5 cut(s) 39, 390, 441, 774, 789
TscAI CASTG 1 cut(s) 180
TseFI GTSAC 2 cut(s) 82, 431
TseI GCWGC 2 cut(s) 265, 656
Tsp45I GTSAC 2 cut(s) 82, 431
TspDTI ATGAA 4 cut(s) 249, 622, 767, 870
TspMI CCCGGG 1 cut(s) 217
TspRI CASTG 1 cut(s) 180
VpaK11BI GGWCC 2 cut(s) 170, 578
XagI CCTNNNNNAGG 1 cut(s) 443
XapI RAATTY 1 cut(s) 241
XmaI CCCGGG 1 cut(s) 217
XspI CTAG 1 cut(s) 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.