Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr2

Orthogroup ID

OG0002643

Physical Location & Seq

Genomic Coordinates Forward (+)

721113 .. 726104

Gene Structure 4992 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3240 bp

ATGAAGCCCAAATCATATCATCCCAAAATAGGAAAAGAGACGAAGTTCCGCTATTCGCACACCGCCCAGAAACCCCTCGATAAACCTCTCTCTCTCTCTCTCTCTCTCTCTCTCAACTCCGAACAGTGCTTCCGACTCTGGGCTCTGCCCTTGAAGCGTTATAGATTTGGTAGGATGGATGACAGTCTGTACGATGAGTTTGGGAATTACATAGGCCCGGAAATCGAGTCTGATCAAGAGAGTGATAGAGAGGACGAGGATGAGGAGCTGCCCGATAGGCAAGAGGATAGGGATGCATCGGATGGCGATGATGCAGCTGCTGGTCCCAATGGGTGGCTGACTGCCTCCAATGATGTCGATATGGACAACCAGGTTGTCCTTGCTGAGGATAAAAAGTATTATCCAACCGCGGAAGACGTCTACGGTGAAGATGTTGAGACATTGGTTATGGATGAAGATGCACAGCCTCTGGAACAACCCATTATTAAGCCTGTTAGGAATGTCAAGTTTGAGGTTGGTGTCAAGGATTCTTCCACCTACGTGTCGAACCAGTTCCTTTTGGGACTTATGTCGAACCCCTCTCTGGTTAGGAATGTAGCACTTGTGGGGCACTTGCAGCACGGGAAGACAGTGTTCATGGATATGTTGGTGGAGCAGACTCACCATATGTCTACTTTTGATTCAAATAGTGATAAGCACATGAGGTACACGGATACAAGGATTGATGAACAAGAGAGGAGGATATCCATTAAGACAGTTCCTATGTCCCTTGTACTCGAGGATAGCAAGTGTAAATCGTATTTGTGCAACATCATGGATACGCCTGGTCATGTCAATTTCTCTGATGAAATGACTGCAGCCCTTAGGCTTGCCGATGGTGTTGTCTTGATTGTGGACGCTGCAGAAGGAATGATGGTAAATACTGAGAGGGCTATACGCCATGCAATCCAAGAGCGCCTACCAATTGTGGTTGTAATCAACAAGGTTGACAGATTGATAACCGAACTCAAATTGCCCCCAAGAGATGCGTACTTCAAGATAAGGCATACTTTGGACGTCATTAATACCAACATAACTGCGGCCTCATCAACTGCTGGCAGTGTACAAATTATTGACCCTGTTGCAGGAAATGTTTGCTTTGCAAGTGCTACCGCAGGATGGTCATTCACATTGCAATCGTTTGCAAAGCTATATCTTAAACTCCATGGGGCTGATAATACGGACGTAGATAAGTTTGCCTCTCGTCTCTGGGGAGATATGTACTATCTACCGGATGACAGGAGTTTTAGGAGAAAACCCCCTGCCAGTGGAGGCGAAAGGTCATTTGTCGAGTTTGTGCTCGAACCCCTGTATAAAATTTATAGCCAGGTGATTGGGGAACATAAAAAGAGTGTGGAGGCAACTCTTGCTGAATTTGGTGTCACCCTCAGCAATGCAGCTTACAAGTTGAATGTTAGACCTCTGCTGAGGTTGGCTTGTAGCTCAGTTTTCGGATATGCCTCAGGCTTTACTGATATGCTGGTTCAACATATTCCTTCTCCTAAAGGTGCCGCAACAAGGAAAGTAGACCATATATATACTGGACCAAAAGACTCTTTGATTTACAAGGCGATGAAAGATTGTGATCCTGATGGTCCTTTGATGGTCAATGTGACAAAATTGTATCCCAAGTCCGACTGTAGTGTGTTTGATGCATTTGGTAGGGTTTATAGTGGCAAGATTCAGACAGGGCAGACTGTACGGGTATTGGGAGAAGGCTATTCACCAGAGGATGAGGAGGACATGACAGTCAAAGAGGTGACTAAGTTATGGCTTTATCAAGCTCGAGACAGGATACCAATAGCCGAGGCTCCTCCTGGTACTTGGGTTCTTATTGAAGGTGTGGATGCTTCTATAATGAAAACTGCCACCATCTGTAATGAATATTATGACGAGGATGTGTACATCTTCCGTCCTCTTCAGTTCAATACTCTGCCAGTTGTGAAAACTGCAACTGAGCCACTAAATCCAAGTGAGCTGCCAAAGATGGTGGAGGGACTAAGAAAGATAAGCAAGAGCTATCCTCTTGCAATCACTAAAGTTGAGGAGTCTGGGGAGCACACGATATTAGGTACAGGAGAACTCTACTTGGATTCAATAATGAAGGATCTTAGAGAGTTATATTCGGAAGTAGAAGTCAAGGTTGCGGATCCTGTTGTTTCATTTTGTGAAACAGTGGTGGAGTCGTCGTCAATGAAATGTTTTGCTGAAACACCCAATAAGAAGAATAAAATAACTATGATTGCAGAGCCATTGGAGAGAGGACTTGCTGAGGACATTGAGAATGGTGTTGTAAGCATTGATTGGTCTCGGAAGGATATTGGCAATTTTTTCCAGACTAGATATGAGTGGGATGTTCTTGCTGCACGGTCCATATGGGCATTTGGCCCTGATAAGCAGGGACCTAACATTCTATTAGATGACACACTTTCCACTGAAGTCGACAAAAGCTTGCTGAATGCTGTGAAGGATTCAATTGTTCAAGGGTTTCAGTGGGGTGCTCGAGAAGGACCCCTTTGTGATGAACCAATCAGAAATGTCAAGTTCAAAATTGTGGATGCAAGGATTGCCCAAGAGCCACTGCATCGTGGATCGATCAACTGGCAAGATCATTCCCACATCTCGACGTGTGGCCTATTCATCTTTCCTAATGGCAACACCGCGTCTTATGGAGCCAGTATATTATGTGGAGGGTTACAAACACCCATCGACTGTATTTCTGCGATCTACACTGTTCTATCTCGCAGGCGTGGACACGTTACAGCTGATGTTCCTCAACCTGGGACCCCTGCTTATATCGTCAAGGCGTTCTTACCGGTGATCGAATCTTTTGGCTTTGAAACTGATTTGAGGTACCATACCCAAGGACAGGCTTTCTGCCTCTCTGTGTTCGATCACTGGGCTATAGTCCCTGGGGATCCACTTGATAAGAGCATCGTTCTGCGACCCCTTGAACCTGCCCCGATTCAGCATCTGGCCCGTGAGTTTATGGTCAAGACAAGACGTAGAAAGGGAATGAGCGAGGATGTGAGCATCAACAAATTCTTCGACGAGGCTATGATGGTGGAGCTGGCTCAGCAGGCAGCTGATCTTCATCTGCAAATGATATCGATCGTGGCGGGGGCTTCACAATACCTTGTCCACATGTCCAAAGTTGAGGAACGAAATTGTACGCGATTTTATTTGGATGAAAACCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005684 GO:0005730 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0007275 GO:0008150 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0010154 GO:0015030 GO:0016020 GO:0016604 GO:0016607 GO:0022414 GO:0030054 GO:0030312 GO:0030532 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043900 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045595 GO:0045694 GO:0046540 GO:0048316 GO:0048367 GO:0048437 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051239 GO:0055044 GO:0061458 GO:0065007 GO:0070013 GO:0071007 GO:0071013 GO:0071944 GO:0090567 GO:0097525 GO:0097526 GO:0099402 GO:0120114 GO:1902494 GO:1990904 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

EFG_C EFG_IV EFTUD2 GTP_EFTU GTP_EFTU_D2

Protein Analysis

1080

Amino Acids

121.07

Weight (kDa)

5.18

Isoelectric Point (pI)

37.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EFTUD2	PF16004	61 - 176	2.1e-36	116 kDa U5 small nuclear ribonucleoprotein component N-terminus
GTP_EFTU	PF00009	196 - 390	3.9e-41	Elongation factor Tu GTP binding domain
GTP_EFTU_D2	PF03144	565 - 639	6.5e-11	Elongation factor Tu domain 2
EFG_IV	PF03764	782 - 879	2.1e-14	Elongation factor G, domain IV
EFG_C	PF00679	912 - 990	2.9e-16	Elongation factor G C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002643)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06220 AT1G06220 AT1G06220 AT5G25230 AT5G25230
fragaria_vesca	FvH4_2g24581 FvH4_7g03790 FvH4_7g03790 FvH4_7g03790
malus_domestica	MD02G1013600.v1.1 MD15G1160400.v1.1
prunus_persica	Prupe.3G036300_v2.0.a1
pyrus_communis	pycom02g01260 pycom15g14420
rosa_chinensis	RchiOBHm_Chr1g0327101 RchiOBHm_Chr1g0327121 RchiOBHm_Chr2g0127751
rosa_laevigata	RLG00000012029 RLG00000012030 RLG00000030043
rosa_multiflora	Rmu_sc0000795.1_g000050 Rmu_sc0004240.1_g000007
rosa_roxburghii	Rroxscaffold_4G00323330 Rroxscaffold_6G00399840 Rroxscaffold_6G00400180
rosa_rugosa	Rorug01G0063500
rosa_samantha	Rh1AG080100 Rh1AG080300 Rh1AG080700 Rh1AG080900 Rh1BG064100 Rh1CG078700 Rh1CG078800 Rh1DG085100 Rh1DG085200 Rh2AG323400 Rh2BG332600 Rh2DG350900
rosa_wichuraiana	Rw1G006400 Rw2G026250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1787
AatII	GACGTC	2 cut(s)	420, 1059
Acc36I	ACCTGC	1 cut(s)	3004
Acc65I	GGTACC	1 cut(s)	2892
AccB1I	GGYRCC	2 cut(s)	1547, 2892
AccB7I	CCANNNNNTGG	1 cut(s)	333
AccI	GTMKAC	4 cut(s)	420, 671, 1566, 2481
AccII	CGCG	3 cut(s)	410, 2702, 3214
AclWI	GGATC	7 cut(s)	1619, 2154, 2183, 2196, 2638, 2951, 2964
AcsI	RAATTY	3 cut(s)	1356, 1412, 3080
AcuI	CTGAAG	2 cut(s)	1943, 2496
AcyI	GRCGYC	2 cut(s)	417, 1056
AflIII	ACRYGT	4 cut(s)	540, 2666, 2794, 3183
AgeI	ACCGGT	1 cut(s)	2854
AjiI	CACGTC	1 cut(s)	2667
AjnI	CCWGG	6 cut(s)	369, 823, 1365, 1855, 2818, 2950
AleI	CACNNNNGTG	1 cut(s)	539
AloI	GAACNNNNNNTCC	2 cut(s)	114, 146
Alw21I	GWGCWC	3 cut(s)	1341, 2100, 2542
Alw26I	GTCTC	5 cut(s)	32, 431, 1250, 1821, 2352
AlwI	GGATC	7 cut(s)	1619, 2154, 2183, 2196, 2638, 2951, 2964
AlwNI	CAGNNNCTG	3 cut(s)	320, 469, 3013
Ama87I	CYCGRG	3 cut(s)	776, 1824, 2541
AoxI	GGCC	5 cut(s)	214, 1080, 2425, 2671, 3015
ApoI	RAATTY	3 cut(s)	1356, 1412, 3080
AseI	ATTAAT	1 cut(s)	1062
AsiGI	ACCGGT	1 cut(s)	2854
Asp700I	GAANNNNTTC	1 cut(s)	551
Asp718I	GGTACC	1 cut(s)	2892
AspLEI	GCGC	1 cut(s)	957
AsuC2I	CCSGG	1 cut(s)	218
AsuHPI	GGTGA	7 cut(s)	437, 653, 1381, 1414, 1755, 1810, 2869
AvaI	CYCGRG	3 cut(s)	776, 1824, 2541
AvaII	GGWCC	7 cut(s)	323, 1583, 1634, 2409, 2441, 2549, 2823
AxyI	CCTNAGG	2 cut(s)	863, 1501
BaeGI	GKGCMC	1 cut(s)	612
BaeI	ACNNNNGTAYC	2 cut(s)	1851, 1884
BamHI	GGATCC	2 cut(s)	2188, 2956
BanI	GGYRCC	2 cut(s)	1547, 2892
BanII	GRGCYC	1 cut(s)	145
BbsI	GAAGAC	2 cut(s)	420, 632
Bbv12I	GWGCWC	3 cut(s)	1341, 2100, 2542
BbvCI	CCTCAGC	4 cut(s)	384, 1427, 1466, 2310
BciT130I	CCWGG	6 cut(s)	371, 825, 1367, 1857, 2820, 2952
BciVI	GTATCC	4 cut(s)	706, 811, 1674, 1827
BclI	TGATCA	1 cut(s)	232
BcnI	CCSGG	1 cut(s)	218
BcoDI	GTCTC	5 cut(s)	32, 431, 1250, 1821, 2352
BfaI	CTAG	1 cut(s)	2379
BfmI	CTRYAG	4 cut(s)	855, 900, 1678, 2943
BfoI	RGCGCY	1 cut(s)	958
BfuAI	ACCTGC	1 cut(s)	3004
BfuI	GTATCC	4 cut(s)	706, 811, 1674, 1827
BglI	GCCNNNNNGGC	1 cut(s)	277
BlpI	GCTNAGC	1 cut(s)	3114
Bme1390I	CCNGG	7 cut(s)	218, 371, 825, 1367, 1857, 2820, 2952
Bme18I	GGWCC	7 cut(s)	323, 1583, 1634, 2409, 2441, 2549, 2823
BmeT110I	CYCGRG	3 cut(s)	776, 1824, 2541
BmgBI	CACGTC	1 cut(s)	2667
BmrFI	CCNGG	7 cut(s)	218, 371, 825, 1367, 1857, 2820, 2952
BmrI	ACTGGG	1 cut(s)	2947
BmuI	ACTGGG	1 cut(s)	2947
BoxI	GACNNNNGTC	1 cut(s)	568
BpiI	GAAGAC	2 cut(s)	420, 632
BplI	GAGNNNNNCTC	2 cut(s)	2047, 2079
Bpu10I	CCTNAGC	4 cut(s)	384, 1427, 1466, 2310
Bpu1102I	GCTNAGC	1 cut(s)	3114
BpuMI	CCSGG	1 cut(s)	218
Bsa29I	ATCGAT	2 cut(s)	2633, 3149
BsaAI	YACGTR	1 cut(s)	541
BsaBI	GATNNNNATC	3 cut(s)	1623, 3132, 3149
BsaHI	GRCGYC	2 cut(s)	417, 1056
BsaI	GGTCTC	1 cut(s)	2352
BsaJI	CCNNGG	7 cut(s)	408, 1204, 1845, 2819, 2902, 2950, 2951
BsaWI	WCCGGW	2 cut(s)	1270, 2854
BsaXI	ACNNNNNCTCC	2 cut(s)	2213, 2243
Bse118I	RCCGGY	1 cut(s)	2854
Bse1I	ACTGG	8 cut(s)	550, 1305, 1585, 1976, 2645, 2715, 2942, 3235
Bse21I	CCTNAGG	2 cut(s)	863, 1501
Bse3DI	GCAATG	2 cut(s)	1169, 1438
Bse8I	GATNNNNATC	3 cut(s)	1623, 3132, 3149
BseBI	CCWGG	6 cut(s)	371, 825, 1367, 1857, 2820, 2952
BseCI	ATCGAT	2 cut(s)	2633, 3149
BseDI	CCNNGG	7 cut(s)	408, 1204, 1845, 2819, 2902, 2950, 2951
BseJI	GATNNNNATC	3 cut(s)	1623, 3132, 3149
BseMI	GCAATG	2 cut(s)	1169, 1438
BseMII	CTCAG	9 cut(s)	375, 915, 1441, 1457, 1497, 1515, 1986, 2301, 3128
BseNI	ACTGG	8 cut(s)	550, 1305, 1585, 1976, 2645, 2715, 2942, 3235
BseRI	GAGGAG	5 cut(s)	278, 751, 1790, 1842, 2099
BseSI	GKGCMC	1 cut(s)	612
BsgI	GTGCAG	1 cut(s)	2388
Bsh1236I	CGCG	3 cut(s)	410, 2702, 3214
Bsh1285I	CGRYCG	1 cut(s)	3153
BshFI	GGCC	5 cut(s)	216, 1082, 2427, 2673, 3017
BshNI	GGYRCC	2 cut(s)	1547, 2892
BshTI	ACCGGT	1 cut(s)	2854
BshVI	ATCGAT	2 cut(s)	2633, 3149
BsiEI	CGRYCG	1 cut(s)	3153
BsiHKAI	GWGCWC	3 cut(s)	1341, 2100, 2542
BsiHKCI	CYCGRG	3 cut(s)	776, 1824, 2541
BsiSI	CCGG	3 cut(s)	218, 1271, 2855
BslFI	GGGAC	7 cut(s)	309, 576, 751, 2049, 2454, 2836, 2933
BsmAI	GTCTC	5 cut(s)	32, 431, 1250, 1821, 2352
BsmBI	CGTCTC	2 cut(s)	32, 1250
BsmFI	GGGAC	7 cut(s)	309, 576, 751, 2049, 2454, 2836, 2933
BsmI	GAATGC	1 cut(s)	2503
BsnI	GGCC	5 cut(s)	216, 1082, 2427, 2673, 3017
Bso31I	GGTCTC	1 cut(s)	2352
BsoBI	CYCGRG	3 cut(s)	776, 1824, 2541
Bsp1286I	GDGCHC	5 cut(s)	145, 612, 1341, 2100, 2542
Bsp1407I	TGTACA	2 cut(s)	1102, 1941
Bsp1720I	GCTNAGC	1 cut(s)	3114
Bsp19I	CCATGG	1 cut(s)	1204
BspANI	GGCC	5 cut(s)	216, 1082, 2427, 2673, 3017
BspCNI	CTCAG	9 cut(s)	376, 916, 1440, 1458, 1496, 1514, 1987, 2302, 3127
BspDI	ATCGAT	2 cut(s)	2633, 3149
BspFNI	CGCG	3 cut(s)	410, 2702, 3214
BspMAI	CTGCAG	2 cut(s)	859, 904
BspMI	ACCTGC	1 cut(s)	3004
BspPI	GGATC	7 cut(s)	1619, 2154, 2183, 2196, 2638, 2951, 2964
BspT107I	GGYRCC	2 cut(s)	1547, 2892
BspTNI	GGTCTC	1 cut(s)	2352
BsrDI	GCAATG	2 cut(s)	1169, 1438
BsrFI	RCCGGY	1 cut(s)	2854
BsrGI	TGTACA	2 cut(s)	1102, 1941
BsrI	ACTGG	8 cut(s)	550, 1305, 1585, 1976, 2645, 2715, 2942, 3235
BssAI	RCCGGY	1 cut(s)	2854
BssECI	CCNNGG	7 cut(s)	408, 1204, 1845, 2819, 2902, 2950, 2951
BssNI	GRCGYC	2 cut(s)	417, 1056
BssT1I	CCWWGG	2 cut(s)	1204, 2902
Bst2UI	CCWGG	6 cut(s)	371, 825, 1367, 1857, 2820, 2952
Bst6I	CTCTTC	1 cut(s)	1962
BstACI	GRCGYC	2 cut(s)	417, 1056
BstAPI	GCANNNNNTGC	2 cut(s)	1406, 2606
BstAUI	TGTACA	2 cut(s)	1102, 1941
BstBAI	YACGTR	1 cut(s)	541
BstC8I	GCNNGC	6 cut(s)	870, 1096, 2492, 2786, 3111, 3120
BstDSI	CCRYGG	2 cut(s)	408, 1204
BstENI	CCTNNNNNAGG	2 cut(s)	383, 1122
BstFNI	CGCG	3 cut(s)	410, 2702, 3214
BstH2I	RGCGCY	1 cut(s)	958
BstHHI	GCGC	1 cut(s)	957
BstMAI	GTCTC	5 cut(s)	32, 431, 1250, 1821, 2352
BstMCI	CGRYCG	1 cut(s)	3153
BstMWI	GCNNNNNNNGC	7 cut(s)	154, 277, 616, 1406, 2606, 3115, 3119
BstNI	CCWGG	6 cut(s)	371, 825, 1367, 1857, 2820, 2952
BstNSI	RCATGY	1 cut(s)	3187
BstPAI	GACNNNNGTC	1 cut(s)	568
BstSCI	CCNGG	7 cut(s)	216, 369, 823, 1365, 1855, 2818, 2950
BstSFI	CTRYAG	4 cut(s)	855, 900, 1678, 2943
BstSLI	GKGCMC	1 cut(s)	612
BstUI	CGCG	3 cut(s)	410, 2702, 3214
BstV2I	GAAGAC	2 cut(s)	420, 632
BstX2I	RGATCY	3 cut(s)	2146, 2188, 2956
BstXI	CCANNNNNNTGG	1 cut(s)	1373
BstYI	RGATCY	3 cut(s)	2146, 2188, 2956
Bsu15I	ATCGAT	2 cut(s)	2633, 3149
Bsu36I	CCTNAGG	2 cut(s)	863, 1501
BsuI	GTATCC	4 cut(s)	706, 811, 1674, 1827
BsuRI	GGCC	5 cut(s)	216, 1082, 2427, 2673, 3017
BsuTUI	ATCGAT	2 cut(s)	2633, 3149
BtgI	CCRYGG	2 cut(s)	408, 1204
BtgZI	GCGATG	2 cut(s)	321, 1625
BtrI	CACGTC	1 cut(s)	2667
BtsI	GCAGTG	2 cut(s)	1105, 2618
BveI	ACCTGC	1 cut(s)	3004
Cac8I	GCNNGC	6 cut(s)	870, 1096, 2492, 2786, 3111, 3120
CaiI	CAGNNNCTG	3 cut(s)	320, 469, 3013
CfoI	GCGC	1 cut(s)	957
Cfr10I	RCCGGY	1 cut(s)	2854
Cfr42I	CCGCGG	1 cut(s)	411
ClaI	ATCGAT	2 cut(s)	2633, 3149
CseI	GACGC	2 cut(s)	905, 2691
CsiI	ACCWGGT	1 cut(s)	369
CspAI	ACCGGT	1 cut(s)	2854
CspCI	CAANNNNNGTGG	2 cut(s)	2010, 2045
CviAII	CATG	8 cut(s)	637, 700, 814, 830, 941, 1205, 1783, 3184
DrdI	GACNNNNNNGTC	1 cut(s)	1787
DseDI	GACNNNNNNGTC	1 cut(s)	1787
Eam1104I	CTCTTC	1 cut(s)	1962
EarI	CTCTTC	1 cut(s)	1962
Eco130I	CCWWGG	2 cut(s)	1204, 2902
Eco24I	GRGCYC	1 cut(s)	145
Eco31I	GGTCTC	1 cut(s)	2352
Eco32I	GATATC	2 cut(s)	744, 3147
Eco47I	GGWCC	7 cut(s)	323, 1583, 1634, 2409, 2441, 2549, 2823
Eco57I	CTGAAG	2 cut(s)	1943, 2496
Eco81I	CCTNAGG	2 cut(s)	863, 1501
Eco88I	CYCGRG	3 cut(s)	776, 1824, 2541
EcoNI	CCTNNNNNAGG	2 cut(s)	383, 1122
EcoO109I	RGGNCCY	3 cut(s)	2441, 2549, 2823
EcoRII	CCWGG	6 cut(s)	369, 823, 1365, 1855, 2818, 2950
EcoRV	GATATC	2 cut(s)	744, 3147
EcoT14I	CCWWGG	2 cut(s)	1204, 2902
EcoT22I	ATGCAT	2 cut(s)	298, 1696
EcoT38I	GRGCYC	1 cut(s)	145
ErhI	CCWWGG	2 cut(s)	1204, 2902
Esp3I	CGTCTC	2 cut(s)	32, 1250
FaeI	CATG	8 cut(s)	640, 703, 817, 833, 944, 1208, 1786, 3187
FalI	AAGNNNNNCTT	6 cut(s)	1033, 1065, 2538, 2570, 2834, 2866
FaqI	GGGAC	7 cut(s)	309, 576, 751, 2049, 2454, 2836, 2933
FatI	CATG	8 cut(s)	636, 699, 813, 829, 940, 1204, 1782, 3183
FauI	CCCGC	1 cut(s)	3151
FauNDI	CATATG	2 cut(s)	666, 2414
FbaI	TGATCA	1 cut(s)	232
FblI	GTMKAC	4 cut(s)	420, 671, 1566, 2481
FriOI	GRGCYC	1 cut(s)	145
FspBI	CTAG	1 cut(s)	2379
GlaI	GCGC	1 cut(s)	956
HaeII	RGCGCY	1 cut(s)	958
HaeIII	GGCC	5 cut(s)	216, 1082, 2427, 2673, 3017
HapII	CCGG	3 cut(s)	218, 1271, 2855
HgaI	GACGC	2 cut(s)	905, 2691
HhaI	GCGC	1 cut(s)	957
Hin1I	GRCGYC	2 cut(s)	417, 1056
Hin1II	CATG	8 cut(s)	640, 703, 817, 833, 944, 1208, 1786, 3187
Hin6I	GCGC	1 cut(s)	955
HinP1I	GCGC	1 cut(s)	955
HincII	GTYRAC	2 cut(s)	988, 2482
HindII	GTYRAC	2 cut(s)	988, 2482
HindIII	AAGCTT	1 cut(s)	2488
HpaII	CCGG	3 cut(s)	218, 1271, 2855
HphI	GGTGA	7 cut(s)	437, 653, 1381, 1414, 1755, 1810, 2869
Hpy99I	CGWCG	3 cut(s)	2230, 2668, 3092
HpyAV	CCTTC	8 cut(s)	899, 1545, 1748, 1871, 2137, 2347, 2500, 2540
HpyCH4IV	ACGT	7 cut(s)	417, 540, 1056, 1224, 2666, 2796, 3043
HpyF10VI	GCNNNNNNNGC	7 cut(s)	154, 277, 616, 1406, 2606, 3115, 3119
HpySE526I	ACGT	7 cut(s)	417, 540, 1056, 1224, 2666, 2796, 3043
Hsp92I	GRCGYC	2 cut(s)	417, 1056
Hsp92II	CATG	8 cut(s)	640, 703, 817, 833, 944, 1208, 1786, 3187
HspAI	GCGC	1 cut(s)	955
KflI	GGGWCCC	1 cut(s)	2823
KpnI	GGTACC	1 cut(s)	2896
Ksp22I	TGATCA	1 cut(s)	232
KspI	CCGCGG	1 cut(s)	411
LmnI	GCTCC	6 cut(s)	265, 652, 1855, 2095, 2711, 3106
MabI	ACCWGGT	1 cut(s)	369
MaeI	CTAG	1 cut(s)	2379
MaeII	ACGT	7 cut(s)	417, 540, 1056, 1224, 2666, 2796, 3043
MaeIII	GTNAC	5 cut(s)	1420, 1651, 1798, 2733, 2797
MfeI	CAATTG	2 cut(s)	963, 2514
MflI	RGATCY	3 cut(s)	2146, 2188, 2956
MhlI	GDGCHC	5 cut(s)	145, 612, 1341, 2100, 2542
MlyI	GAGTC	6 cut(s)	129, 236, 652, 1586, 2096, 2231
MmeI	TCCRAC	3 cut(s)	157, 428, 1698
Mph1103I	ATGCAT	2 cut(s)	298, 1696
MroXI	GAANNNNTTC	1 cut(s)	551
MseI	TTAA	4 cut(s)	486, 750, 1062, 1197
MslI	CAYNNNNRTG	2 cut(s)	539, 641
MspA1I	CMGCKG	4 cut(s)	317, 410, 2804, 3125
MspI	CCGG	3 cut(s)	218, 1271, 2855
MspR9I	CCNGG	7 cut(s)	218, 371, 825, 1367, 1857, 2820, 2952
MunI	CAATTG	2 cut(s)	963, 2514
Mva1269I	GAATGC	1 cut(s)	2503
MvaI	CCWGG	6 cut(s)	371, 825, 1367, 1857, 2820, 2952
MvnI	CGCG	3 cut(s)	410, 2702, 3214
MwoI	GCNNNNNNNGC	7 cut(s)	154, 277, 616, 1406, 2606, 3115, 3119
NciI	CCSGG	1 cut(s)	218
NcoI	CCATGG	1 cut(s)	1204
NdeI	CATATG	2 cut(s)	666, 2414
NlaIII	CATG	8 cut(s)	640, 703, 817, 833, 944, 1208, 1786, 3187
NmeAIII	GCCGAG	1 cut(s)	1870
NmuCI	GTSAC	3 cut(s)	1420, 1651, 1798
NsiI	ATGCAT	2 cut(s)	298, 1696
NspI	RCATGY	1 cut(s)	3187
OliI	CACNNNNGTG	1 cut(s)	539
PaeR7I	CTCGAG	3 cut(s)	776, 1824, 2541
PasI	CCCWGGG	1 cut(s)	2951
PciI	ACATGT	1 cut(s)	3183
PctI	GAATGC	1 cut(s)	2503
PdmI	GAANNNNTTC	1 cut(s)	551
PfeI	GAWTC	7 cut(s)	527, 680, 1720, 2132, 2510, 2864, 3004
PflMI	CCANNNNNTGG	1 cut(s)	333
PinAI	ACCGGT	1 cut(s)	2854
Ple19I	CGATCG	1 cut(s)	3153
PleI	GAGTC	6 cut(s)	129, 235, 652, 1586, 2095, 2230
PpsI	GAGTC	6 cut(s)	129, 235, 652, 1586, 2095, 2230
Ppu21I	YACGTR	1 cut(s)	541
PpuMI	RGGWCCY	3 cut(s)	2441, 2549, 2823
PscI	ACATGT	1 cut(s)	3183
PshAI	GACNNNNGTC	1 cut(s)	568
PshBI	ATTAAT	1 cut(s)	1062
Psp5II	RGGWCCY	3 cut(s)	2441, 2549, 2823
Psp6I	CCWGG	6 cut(s)	369, 823, 1365, 1855, 2818, 2950
PspGI	CCWGG	6 cut(s)	369, 823, 1365, 1855, 2818, 2950
PspPPI	RGGWCCY	3 cut(s)	2441, 2549, 2823
PspXI	VCTCGAGB	1 cut(s)	776
PstI	CTGCAG	2 cut(s)	859, 904
PstNI	CAGNNNCTG	3 cut(s)	320, 469, 3013
PsuI	RGATCY	3 cut(s)	2146, 2188, 2956
PvuI	CGATCG	1 cut(s)	3153
PvuII	CAGCTG	3 cut(s)	317, 2804, 3125
RseI	CAYNNNNRTG	2 cut(s)	539, 641
SacII	CCGCGG	1 cut(s)	411
SalI	GTCGAC	1 cut(s)	2480
SaqAI	TTAA	4 cut(s)	486, 750, 1062, 1197
SchI	GAGTC	6 cut(s)	129, 236, 652, 1586, 2096, 2231
ScrFI	CCNGG	7 cut(s)	218, 371, 825, 1367, 1857, 2820, 2952
SduI	GDGCHC	5 cut(s)	145, 612, 1341, 2100, 2542
SexAI	ACCWGGT	1 cut(s)	369
SfcI	CTRYAG	4 cut(s)	855, 900, 1678, 2943
Sfr274I	CTCGAG	3 cut(s)	776, 1824, 2541
Sfr303I	CCGCGG	1 cut(s)	411
SgrBI	CCGCGG	1 cut(s)	411
SinI	GGWCC	7 cut(s)	323, 1583, 1634, 2409, 2441, 2549, 2823
SlaI	CTCGAG	3 cut(s)	776, 1824, 2541
SmiMI	CAYNNNNRTG	2 cut(s)	539, 641
SmlI	CTYRAG	3 cut(s)	776, 1824, 2541
SmoI	CTYRAG	3 cut(s)	776, 1824, 2541
SspI	AATATT	1 cut(s)	1925
SspMI	CTAG	1 cut(s)	2379
StyD4I	CCNGG	7 cut(s)	216, 369, 823, 1365, 1855, 2818, 2950
StyI	CCWWGG	2 cut(s)	1204, 2902
TaiI	ACGT	7 cut(s)	420, 543, 1059, 1227, 2669, 2799, 3046
TatI	WGTACW	4 cut(s)	772, 1102, 1260, 1941
TauI	GCSGC	2 cut(s)	1082, 1553
TfiI	GAWTC	7 cut(s)	527, 680, 1720, 2132, 2510, 2864, 3004
Tru1I	TTAA	4 cut(s)	486, 750, 1062, 1197
Tru9I	TTAA	4 cut(s)	486, 750, 1062, 1197
TseFI	GTSAC	3 cut(s)	1420, 1651, 1798
Tsp45I	GTSAC	3 cut(s)	1420, 1651, 1798
TspGWI	ACGGA	3 cut(s)	725, 1235, 1940
Van91I	CCANNNNNTGG	1 cut(s)	333
VpaK11BI	GGWCC	7 cut(s)	323, 1583, 1634, 2409, 2441, 2549, 2823
VspI	ATTAAT	1 cut(s)	1062
XagI	CCTNNNNNAGG	2 cut(s)	383, 1122
XapI	RAATTY	3 cut(s)	1356, 1412, 3080
XceI	RCATGY	1 cut(s)	3187
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1577
XhoI	CTCGAG	3 cut(s)	776, 1824, 2541
XmiI	GTMKAC	4 cut(s)	420, 671, 1566, 2481
XmnI	GAANNNNTTC	1 cut(s)	551
XspI	CTAG	1 cut(s)	2379
ZraI	GACGTC	2 cut(s)	418, 1057
Zsp2I	ATGCAT	2 cut(s)	298, 1696

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.