pycom02g19010

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0008161
Physical Location & Seq
Forward (+)
17044490 .. 17044933
444 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 444 bp
ATGAGCTCGGCGTCGGAAGCGGCGATCGAGGAGCGACATAACGGGCAAGTCTGCTGCGACTGGAGCCACGTGTCGATGCAGACGGCGTGGAAGGCGTGGCAGCAGAGCGGGAGGAGACGGAGCTCGTCGCTGGGCTCGAATTTCGACAGGCAGACTGCGCAGTCGGCGGATATGGTGGTGGAGGATCGGCGAGTGATGGAGGAGAAGGTGAAGAGAGGGAGGGCGGGCAAGGTCACCGGATTGACTCGGCGAGTGGATATGCGGCGAGAATCGGAGGATGAGACGGCGGGGGAGGGCGCAAGGCGACGGATACATCGGCGGTTGAGGTGGCGGAGGATGAGACAGAGGCAGATTGAGGCGACGAGTGTGGCTGTGAGGATGATAATGATGATGAAGAGGCTCGGGGTTAGGGTTTTGAAGGAGGAGGAGGAGGATTGGCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

148

Amino Acids

17.32

Weight (kDa)

11.02

Isoelectric Point (pI)

94.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 160
Acc16I TGCGCA 1 cut(s) 159
AccBSI CCGCTC 1 cut(s) 108
AciI CCGC 9 cut(s) 20, 108, 167, 224, 262, 287, 319, 331, 439
AclWI GGATC 1 cut(s) 192
AcsI RAATTY 1 cut(s) 139
AcvI CACGTG 1 cut(s) 70
AcyI GRCGYC 1 cut(s) 11
AflIII ACRYGT 1 cut(s) 69
AgsI TTSAA 1 cut(s) 418
AluBI AGCT 2 cut(s) 6, 123
AluI AGCT 2 cut(s) 6, 123
Alw21I GWGCWC 2 cut(s) 8, 125
Alw26I GTCTC 3 cut(s) 109, 275, 334
AlwI GGATC 1 cut(s) 192
Ama87I CYCGRG 1 cut(s) 401
ApeKI GCWGC 2 cut(s) 54, 100
ApoI RAATTY 1 cut(s) 139
AspLEI GCGC 2 cut(s) 160, 299
AsuHPI GGTGA 2 cut(s) 220, 226
AvaI CYCGRG 1 cut(s) 401
BanII GRGCYC 3 cut(s) 8, 125, 137
BbrPI CACGTG 1 cut(s) 70
Bbv12I GWGCWC 2 cut(s) 8, 125
BbvI GCAGC 2 cut(s) 41, 112
BccI CCATC 1 cut(s) 190
BceAI ACGGC 2 cut(s) 99, 300
BciVI GTATCC 1 cut(s) 303
BcoDI GTCTC 3 cut(s) 109, 275, 334
BfuI GTATCC 1 cut(s) 303
BisI GCNGC 4 cut(s) 21, 55, 101, 263
BlsI GCNGC 4 cut(s) 22, 56, 102, 264
BmeT110I CYCGRG 1 cut(s) 401
BmiI GGNNCC 1 cut(s) 65
BmsI GCATC 1 cut(s) 66
BpmI CTGGAG 1 cut(s) 82
BsaAI YACGTR 1 cut(s) 70
BsaHI GRCGYC 1 cut(s) 11
BsaWI WCCGGW 1 cut(s) 236
Bse1I ACTGG 1 cut(s) 65
BseGI GGATG 3 cut(s) 283, 342, 384
BseNI ACTGG 1 cut(s) 65
BseRI GAGGAG 6 cut(s) 44, 127, 215, 437, 440, 443
BseXI GCAGC 2 cut(s) 41, 112
BseYI CCCAGC 1 cut(s) 130
Bsh1285I CGRYCG 1 cut(s) 27
BsiEI CGRYCG 1 cut(s) 27
BsiHKAI GWGCWC 2 cut(s) 8, 125
BsiHKCI CYCGRG 1 cut(s) 401
BsiSI CCGG 1 cut(s) 237
BsmAI GTCTC 3 cut(s) 109, 275, 334
BsmBI CGTCTC 2 cut(s) 109, 275
BsoBI CYCGRG 1 cut(s) 401
Bsp1286I GDGCHC 3 cut(s) 8, 125, 137
Bsp143I GATC 2 cut(s) 24, 184
BspACI CCGC 9 cut(s) 20, 108, 167, 224, 262, 287, 319, 331, 439
BspLI GGNNCC 1 cut(s) 65
BspPI GGATC 1 cut(s) 192
BsrBI CCGCTC 1 cut(s) 108
BsrI ACTGG 1 cut(s) 65
BssMI GATC 2 cut(s) 24, 184
BssNI GRCGYC 1 cut(s) 11
Bst6I CTCTTC 2 cut(s) 206, 389
BstACI GRCGYC 1 cut(s) 11
BstBAI YACGTR 1 cut(s) 70
BstC8I GCNNGC 1 cut(s) 226
BstEII GGTNACC 1 cut(s) 232
BstF5I GGATG 3 cut(s) 283, 342, 384
BstHHI GCGC 2 cut(s) 160, 299
BstKTI GATC 2 cut(s) 27, 187
BstMAI GTCTC 3 cut(s) 109, 275, 334
BstMBI GATC 2 cut(s) 24, 184
BstMCI CGRYCG 1 cut(s) 27
BstMWI GCNNNNNNNGC 5 cut(s) 17, 63, 92, 157, 164
BstPI GGTNACC 1 cut(s) 232
BstV1I GCAGC 2 cut(s) 41, 112
BsuI GTATCC 1 cut(s) 303
BtsCI GGATG 3 cut(s) 283, 342, 384
Cac8I GCNNGC 1 cut(s) 226
CfoI GCGC 2 cut(s) 160, 299
CviJI RGCY 6 cut(s) 6, 66, 123, 135, 371, 400
CviKI_1 RGCY 6 cut(s) 6, 66, 123, 135, 371, 400
DpnI GATC 2 cut(s) 26, 186
DpnII GATC 2 cut(s) 24, 184
DrdI GACNNNNNNGTC 1 cut(s) 160
DseDI GACNNNNNNGTC 1 cut(s) 160
Eam1104I CTCTTC 2 cut(s) 206, 389
EarI CTCTTC 2 cut(s) 206, 389
EciI GGCGGA 2 cut(s) 182, 346
Ecl136II GAGCTC 2 cut(s) 6, 123
Eco24I GRGCYC 3 cut(s) 8, 125, 137
Eco53kI GAGCTC 2 cut(s) 6, 123
Eco72I CACGTG 1 cut(s) 70
Eco88I CYCGRG 1 cut(s) 401
Eco91I GGTNACC 1 cut(s) 232
EcoICRI GAGCTC 2 cut(s) 6, 123
EcoO65I GGTNACC 1 cut(s) 232
EcoT38I GRGCYC 3 cut(s) 8, 125, 137
Esp3I CGTCTC 2 cut(s) 109, 275
FaiI YATR 3 cut(s) 39, 173, 260
FauI CCCGC 3 cut(s) 101, 217, 280
Fnu4HI GCNGC 4 cut(s) 21, 55, 101, 263
FokI GGATG 3 cut(s) 290, 349, 391
FriOI GRGCYC 3 cut(s) 8, 125, 137
Fsp4HI GCNGC 4 cut(s) 21, 55, 101, 263
FspI TGCGCA 1 cut(s) 159
GlaI GCGC 2 cut(s) 159, 298
GluI GCNGC 4 cut(s) 21, 55, 101, 263
GsaI CCCAGC 1 cut(s) 134
GsuI CTGGAG 1 cut(s) 82
HapII CCGG 1 cut(s) 237
HhaI GCGC 2 cut(s) 160, 299
Hin1I GRCGYC 1 cut(s) 11
Hin6I GCGC 2 cut(s) 158, 297
HinP1I GCGC 2 cut(s) 158, 297
HinfI GANTC 2 cut(s) 244, 269
HpaII CCGG 1 cut(s) 237
HphI GGTGA 2 cut(s) 220, 226
Hpy188I TCNGA 2 cut(s) 16, 274
Hpy99I CGWCG 4 cut(s) 16, 130, 309, 364
HpyAV CCTTC 3 cut(s) 85, 199, 412
HpyCH4IV ACGT 1 cut(s) 69
HpyCH4V TGCA 1 cut(s) 79
HpyF10VI GCNNNNNNNGC 5 cut(s) 17, 63, 92, 157, 164
HpySE526I ACGT 1 cut(s) 69
Hsp92I GRCGYC 1 cut(s) 11
HspAI GCGC 2 cut(s) 158, 297
Kzo9I GATC 2 cut(s) 24, 184
LmnI GCTCC 3 cut(s) 31, 63, 120
LpnPI CCDG 4 cut(s) 46, 116, 133, 250
Lsp1109I GCAGC 2 cut(s) 41, 112
LweI GCATC 1 cut(s) 66
MaeII ACGT 1 cut(s) 69
MaeIII GTNAC 1 cut(s) 232
MalI GATC 2 cut(s) 26, 186
MbiI CCGCTC 1 cut(s) 108
MboI GATC 2 cut(s) 24, 184
MboII GAAGA 2 cut(s) 223, 406
MhlI GDGCHC 3 cut(s) 8, 125, 137
MluCI AATT 1 cut(s) 139
MlyI GAGTC 1 cut(s) 238
MspI CCGG 1 cut(s) 237
MwoI GCNNNNNNNGC 5 cut(s) 17, 63, 92, 157, 164
NdeII GATC 2 cut(s) 24, 184
NlaIV GGNNCC 1 cut(s) 65
NmeAIII GCCGAG 1 cut(s) 226
NmuCI GTSAC 1 cut(s) 232
NsbI TGCGCA 1 cut(s) 159
PcsI WCGNNNNNNNCGW 2 cut(s) 20, 134
PfeI GAWTC 1 cut(s) 269
PkrI GCNGC 4 cut(s) 22, 56, 102, 264
Ple19I CGATCG 1 cut(s) 27
PleI GAGTC 1 cut(s) 238
PmaCI CACGTG 1 cut(s) 70
PmlI CACGTG 1 cut(s) 70
PpsI GAGTC 1 cut(s) 238
Ppu21I YACGTR 1 cut(s) 70
Psp124BI GAGCTC 2 cut(s) 8, 125
PspCI CACGTG 1 cut(s) 70
PspEI GGTNACC 1 cut(s) 232
PspFI CCCAGC 1 cut(s) 130
PspN4I GGNNCC 1 cut(s) 65
PvuI CGATCG 1 cut(s) 27
SacI GAGCTC 2 cut(s) 8, 125
SatI GCNGC 4 cut(s) 21, 55, 101, 263
Sau3AI GATC 2 cut(s) 24, 184
SchI GAGTC 1 cut(s) 238
SduI GDGCHC 3 cut(s) 8, 125, 137
SetI ASST 6 cut(s) 8, 72, 125, 210, 234, 329
SfaNI GCATC 1 cut(s) 66
Sse9I AATT 1 cut(s) 139
SsiI CCGC 9 cut(s) 20, 108, 167, 224, 262, 287, 319, 331, 439
SstI GAGCTC 2 cut(s) 8, 125
TaiI ACGT 1 cut(s) 72
TaqI TCGA 4 cut(s) 27, 74, 137, 144
TasI AATT 1 cut(s) 139
TauI GCSGC 2 cut(s) 23, 265
TfiI GAWTC 1 cut(s) 269
TseFI GTSAC 1 cut(s) 232
TseI GCWGC 2 cut(s) 54, 100
Tsp45I GTSAC 1 cut(s) 232
TspDTI ATGAA 1 cut(s) 407
TspGWI ACGGA 2 cut(s) 133, 322
XapI RAATTY 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.