RLG00000028896

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0008161
Physical Location & Seq
Forward (+)
29940352 .. 29941168
817 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028896

Sequence Viewer

Length: 438 bp
ATGCACGATACGTGGGAAGGCTTACGTGACAGCAGAGAGGGAGTAGCCGGAGCTGATCGTCGTGCTCGAACTTCGAGGTCGGAAGCGACGACGGCAATGGAGGAGGCTCGGCGGTGCTTGATAGGGGAGAAGGCGAAGACGGGGAGCGAGGCGGCGGCAGATTCGGCCGGAGATACTCGGGCGGGGGCGTCGTCGCCAGAAGAGGGAGTGGTGGAGATTGATAATGGCGGTGGCGGCGGAGTTCTTGGCGAAGATCGGGAAGATCGGCTTCAGAATGTCAGCATTCCTGTTGACGTTTCTCTTGGGCTTGATCGCTTTGGCGTCTGCTTACTCGGAGACACCAAGTCCTATGATGAGTGTGCGACTATGATCCAAGTTTATGATACTGATGGAAATGGGGTGCTTGATTTTAATGAGTTTCATCGAATTATGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_6 EF-hand_8

Protein Analysis

146

Amino Acids

15.44

Weight (kDa)

4.47

Isoelectric Point (pI)

48.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 120 - 144 1.2e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 7 cut(s) 112, 152, 155, 182, 228, 234, 237
AclWI GGATC 1 cut(s) 364
AcoI YGGCCR 1 cut(s) 165
AcuI CTGAAG 1 cut(s) 254
AcyI GRCGYC 2 cut(s) 188, 321
AfiI CCNNNNNNNGG 1 cut(s) 203
AhdI GACNNNNNGTC 1 cut(s) 343
AluBI AGCT 1 cut(s) 53
AluI AGCT 1 cut(s) 53
Alw21I GWGCWC 1 cut(s) 67
Alw26I GTCTC 1 cut(s) 330
AlwI GGATC 1 cut(s) 364
Ama87I CYCGRG 1 cut(s) 177
AoxI GGCC 1 cut(s) 165
AvaI CYCGRG 1 cut(s) 177
BbsI GAAGAC 1 cut(s) 143
Bbv12I GWGCWC 1 cut(s) 67
BccI CCATC 1 cut(s) 383
BceAI ACGGC 1 cut(s) 108
BcoDI GTCTC 1 cut(s) 330
BisI GCNGC 3 cut(s) 153, 156, 235
BlsI GCNGC 3 cut(s) 154, 157, 236
BmeRI GACNNNNNGTC 1 cut(s) 343
BmeT110I CYCGRG 1 cut(s) 177
BpiI GAAGAC 1 cut(s) 143
BsaAI YACGTR 2 cut(s) 12, 26
BsaHI GRCGYC 2 cut(s) 188, 321
Bsc4I CCNNNNNNNGG 1 cut(s) 203
Bse3DI GCAATG 1 cut(s) 102
BseLI CCNNNNNNNGG 1 cut(s) 203
BseMI GCAATG 1 cut(s) 102
BseRI GAGGAG 1 cut(s) 116
BseX3I CGGCCG 1 cut(s) 165
Bsh1285I CGRYCG 1 cut(s) 168
BshFI GGCC 1 cut(s) 167
BsiEI CGRYCG 1 cut(s) 168
BsiHKAI GWGCWC 1 cut(s) 67
BsiHKCI CYCGRG 1 cut(s) 177
BsiSI CCGG 2 cut(s) 48, 168
BslI CCNNNNNNNGG 1 cut(s) 203
BsmAI GTCTC 1 cut(s) 330
BsmI GAATGC 1 cut(s) 282
BsnI GGCC 1 cut(s) 167
BsoBI CYCGRG 1 cut(s) 177
Bsp1286I GDGCHC 1 cut(s) 67
Bsp143I GATC 5 cut(s) 55, 253, 262, 310, 369
BspACI CCGC 7 cut(s) 112, 152, 155, 182, 228, 234, 237
BspANI GGCC 1 cut(s) 167
BspPI GGATC 1 cut(s) 364
BsrDI GCAATG 1 cut(s) 102
BssMI GATC 5 cut(s) 55, 253, 262, 310, 369
BssNI GRCGYC 2 cut(s) 188, 321
Bst6I CTCTTC 1 cut(s) 195
BstACI GRCGYC 2 cut(s) 188, 321
BstBAI YACGTR 2 cut(s) 12, 26
BstKTI GATC 5 cut(s) 58, 256, 265, 313, 372
BstMAI GTCTC 1 cut(s) 330
BstMBI GATC 5 cut(s) 55, 253, 262, 310, 369
BstMCI CGRYCG 1 cut(s) 168
BstMWI GCNNNNNNNGC 3 cut(s) 92, 164, 234
BstV2I GAAGAC 1 cut(s) 143
BstZI CGGCCG 1 cut(s) 165
BsuRI GGCC 1 cut(s) 167
CseI GACGC 2 cut(s) 177, 310
CviJI RGCY 8 cut(s) 21, 47, 53, 107, 167, 268, 307, 434
CviKI_1 RGCY 8 cut(s) 21, 47, 53, 107, 167, 268, 307, 434
DpnI GATC 5 cut(s) 57, 255, 264, 312, 371
DpnII GATC 5 cut(s) 55, 253, 262, 310, 369
DriI GACNNNNNGTC 1 cut(s) 343
EaeI YGGCCR 1 cut(s) 165
EagI CGGCCG 1 cut(s) 165
Eam1104I CTCTTC 1 cut(s) 195
Eam1105I GACNNNNNGTC 1 cut(s) 343
EarI CTCTTC 1 cut(s) 195
EciI GGCGGA 1 cut(s) 252
EclXI CGGCCG 1 cut(s) 165
Eco52I CGGCCG 1 cut(s) 165
Eco57I CTGAAG 1 cut(s) 254
Eco88I CYCGRG 1 cut(s) 177
FaiI YATR 4 cut(s) 351, 368, 381, 431
FalI AAGNNNNNCTT 2 cut(s) 252, 284
FauI CCCGC 1 cut(s) 175
Fnu4HI GCNGC 3 cut(s) 153, 156, 235
Fsp4HI GCNGC 3 cut(s) 153, 156, 235
GluI GCNGC 3 cut(s) 153, 156, 235
HaeIII GGCC 1 cut(s) 167
HapII CCGG 2 cut(s) 48, 168
HgaI GACGC 2 cut(s) 177, 310
Hin1I GRCGYC 2 cut(s) 188, 321
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HinfI GANTC 1 cut(s) 161
HpaII CCGG 2 cut(s) 48, 168
Hpy166II GTNNAC 1 cut(s) 292
Hpy188I TCNGA 3 cut(s) 82, 273, 335
Hpy188III TCNNGA 1 cut(s) 257
Hpy8I GTNNAC 1 cut(s) 292
Hpy99I CGWCG 5 cut(s) 63, 91, 94, 193, 196
HpyAV CCTTC 2 cut(s) 11, 124
HpyCH4IV ACGT 3 cut(s) 11, 25, 294
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 3 cut(s) 92, 164, 234
HpySE526I ACGT 3 cut(s) 11, 25, 294
Hsp92I GRCGYC 2 cut(s) 188, 321
Kzo9I GATC 5 cut(s) 55, 253, 262, 310, 369
LmnI GCTCC 2 cut(s) 50, 144
LpnPI CCDG 4 cut(s) 61, 181, 210, 300
MaeII ACGT 3 cut(s) 11, 25, 294
MaeIII GTNAC 1 cut(s) 26
MalI GATC 5 cut(s) 57, 255, 264, 312, 371
MboI GATC 5 cut(s) 55, 253, 262, 310, 369
MboII GAAGA 4 cut(s) 148, 212, 263, 272
MhlI GDGCHC 1 cut(s) 67
MluCI AATT 1 cut(s) 426
MmeI TCCRAC 1 cut(s) 60
MnlI CCTC 6 cut(s) 31, 69, 94, 97, 142, 196
MseI TTAA 2 cut(s) 411, 436
MspI CCGG 2 cut(s) 48, 168
Mva1269I GAATGC 1 cut(s) 282
MwoI GCNNNNNNNGC 3 cut(s) 92, 164, 234
NdeII GATC 5 cut(s) 55, 253, 262, 310, 369
NmeAIII GCCGAG 1 cut(s) 88
NmuCI GTSAC 1 cut(s) 26
PcsI WCGNNNNNNNCGW 2 cut(s) 64, 86
PctI GAATGC 1 cut(s) 282
PfeI GAWTC 1 cut(s) 161
PkrI GCNGC 3 cut(s) 154, 157, 236
Ppu21I YACGTR 2 cut(s) 12, 26
SaqAI TTAA 2 cut(s) 411, 436
SatI GCNGC 3 cut(s) 153, 156, 235
Sau3AI GATC 5 cut(s) 55, 253, 262, 310, 369
SduI GDGCHC 1 cut(s) 67
SetI ASST 5 cut(s) 14, 28, 55, 80, 297
Sse9I AATT 1 cut(s) 426
SsiI CCGC 7 cut(s) 112, 152, 155, 182, 228, 234, 237
TaiI ACGT 3 cut(s) 14, 28, 297
TaqI TCGA 3 cut(s) 67, 74, 424
TasI AATT 1 cut(s) 426
TauI GCSGC 3 cut(s) 155, 158, 237
TfiI GAWTC 1 cut(s) 161
Tru1I TTAA 2 cut(s) 411, 436
Tru9I TTAA 2 cut(s) 411, 436
TseFI GTSAC 1 cut(s) 26
Tsp45I GTSAC 1 cut(s) 26
TspDTI ATGAA 1 cut(s) 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.