Rh4DG034400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0008161
Physical Location & Seq
Reverse (-)
5856273 .. 5856554
282 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG034400.1

Sequence Viewer

Length: 282 bp
ATGGCGGAGAAGGCGAAGACCGGGAGTGAGGCGACGGGATTGGCGGCGGCAGCGGATTCGGCCGAAGATACTCGGGCGGGGGCGTCGTCGCCGGAAAAGGGAGTCCGGGTCGGATGTGAAGAGCGGGTCGGAGAATTCTTCAAGGAAGACGATGAGCTGGAGCTTGAGAGGGAATTCTCGCTTGTTGAGACAAGAGAGGGCTTGGTCGATGATGATTGGGTGCGCGGAGGTCCAGCGGTGACTGGAAATCGTCGATTAAGGACTCCCAGTCTTGGAGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

93

Amino Acids

9.91

Weight (kDa)

4.55

Isoelectric Point (pI)

27.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 124
AccII CGCG 1 cut(s) 225
AciI CCGC 8 cut(s) 5, 44, 47, 53, 77, 124, 225, 236
AcoI YGGCCR 1 cut(s) 60
AcsI RAATTY 2 cut(s) 134, 173
AcyI GRCGYC 1 cut(s) 83
AfiI CCNNNNNNNGG 2 cut(s) 98, 272
AgsI TTSAA 1 cut(s) 142
AhdI GACNNNNNGTC 1 cut(s) 267
AluBI AGCT 2 cut(s) 157, 163
AluI AGCT 2 cut(s) 157, 163
Alw26I GTCTC 1 cut(s) 182
Ama87I CYCGRG 1 cut(s) 72
AoxI GGCC 1 cut(s) 60
ApeKI GCWGC 1 cut(s) 50
ApoI RAATTY 2 cut(s) 134, 173
AspLEI GCGC 1 cut(s) 225
AspS9I GGNCC 1 cut(s) 230
AsuC2I CCSGG 2 cut(s) 22, 107
AsuHPI GGTGA 1 cut(s) 250
AvaI CYCGRG 1 cut(s) 72
AvaII GGWCC 1 cut(s) 230
BbsI GAAGAC 2 cut(s) 23, 153
BbvI GCAGC 1 cut(s) 62
BcgI CGANNNNNNTGC 2 cut(s) 39, 73
BcnI CCSGG 2 cut(s) 22, 107
BcoDI GTCTC 1 cut(s) 182
BisI GCNGC 3 cut(s) 45, 48, 51
BlsI GCNGC 3 cut(s) 46, 49, 52
Bme1390I CCNGG 2 cut(s) 22, 107
Bme18I GGWCC 1 cut(s) 230
BmeRI GACNNNNNGTC 1 cut(s) 267
BmeT110I CYCGRG 1 cut(s) 72
BmgT120I GGNCC 1 cut(s) 230
BmrFI CCNGG 2 cut(s) 22, 107
BmrI ACTGGG 1 cut(s) 261
BmuI ACTGGG 1 cut(s) 261
BpiI GAAGAC 2 cut(s) 23, 153
BpmI CTGGAG 1 cut(s) 179
BpuEI CTTGAG 1 cut(s) 185
BpuMI CCSGG 2 cut(s) 22, 107
BsaHI GRCGYC 1 cut(s) 83
BsaXI ACNNNNNCTCC 2 cut(s) 93, 123
Bsc4I CCNNNNNNNGG 2 cut(s) 98, 272
Bse1I ACTGG 2 cut(s) 247, 267
BseGI GGATG 1 cut(s) 119
BseLI CCNNNNNNNGG 2 cut(s) 98, 272
BseNI ACTGG 2 cut(s) 247, 267
BseX3I CGGCCG 1 cut(s) 60
BseXI GCAGC 1 cut(s) 62
Bsh1236I CGCG 1 cut(s) 225
Bsh1285I CGRYCG 1 cut(s) 63
BshFI GGCC 1 cut(s) 62
BsiEI CGRYCG 1 cut(s) 63
BsiHKCI CYCGRG 1 cut(s) 72
BsiSI CCGG 3 cut(s) 21, 92, 106
BslI CCNNNNNNNGG 2 cut(s) 98, 272
BsmAI GTCTC 1 cut(s) 182
BsnI GGCC 1 cut(s) 62
BsoBI CYCGRG 1 cut(s) 72
BspACI CCGC 8 cut(s) 5, 44, 47, 53, 77, 124, 225, 236
BspANI GGCC 1 cut(s) 62
BspFNI CGCG 1 cut(s) 225
BspQI GCTCTTC 1 cut(s) 114
BsrBI CCGCTC 1 cut(s) 124
BsrI ACTGG 2 cut(s) 247, 267
BssNI GRCGYC 1 cut(s) 83
Bst6I CTCTTC 1 cut(s) 114
BstACI GRCGYC 1 cut(s) 83
BstF5I GGATG 1 cut(s) 119
BstFNI CGCG 1 cut(s) 225
BstHHI GCGC 1 cut(s) 225
BstMAI GTCTC 1 cut(s) 182
BstMCI CGRYCG 1 cut(s) 63
BstMWI GCNNNNNNNGC 3 cut(s) 11, 50, 59
BstSCI CCNGG 2 cut(s) 20, 105
BstUI CGCG 1 cut(s) 225
BstV1I GCAGC 1 cut(s) 62
BstV2I GAAGAC 2 cut(s) 23, 153
BstZI CGGCCG 1 cut(s) 60
BsuRI GGCC 1 cut(s) 62
BtsCI GGATG 1 cut(s) 119
CfoI GCGC 1 cut(s) 225
Cfr13I GGNCC 1 cut(s) 230
CseI GACGC 1 cut(s) 72
CviJI RGCY 4 cut(s) 62, 157, 163, 201
CviKI_1 RGCY 4 cut(s) 62, 157, 163, 201
DriI GACNNNNNGTC 1 cut(s) 267
EaeI YGGCCR 1 cut(s) 60
EagI CGGCCG 1 cut(s) 60
Eam1104I CTCTTC 1 cut(s) 114
Eam1105I GACNNNNNGTC 1 cut(s) 267
EarI CTCTTC 1 cut(s) 114
EciI GGCGGA 1 cut(s) 20
EclXI CGGCCG 1 cut(s) 60
Eco47I GGWCC 1 cut(s) 230
Eco52I CGGCCG 1 cut(s) 60
Eco88I CYCGRG 1 cut(s) 72
EcoRI GAATTC 2 cut(s) 134, 173
FauI CCCGC 2 cut(s) 70, 117
Fnu4HI GCNGC 3 cut(s) 45, 48, 51
FokI GGATG 1 cut(s) 126
Fsp4HI GCNGC 3 cut(s) 45, 48, 51
GlaI GCGC 1 cut(s) 224
GluI GCNGC 3 cut(s) 45, 48, 51
GsuI CTGGAG 1 cut(s) 179
HaeIII GGCC 1 cut(s) 62
HapII CCGG 3 cut(s) 21, 92, 106
HgaI GACGC 1 cut(s) 72
HhaI GCGC 1 cut(s) 225
Hin1I GRCGYC 1 cut(s) 83
Hin6I GCGC 1 cut(s) 223
HinP1I GCGC 1 cut(s) 223
HinfI GANTC 3 cut(s) 56, 102, 262
HpaII CCGG 3 cut(s) 21, 92, 106
HphI GGTGA 1 cut(s) 250
Hpy188I TCNGA 2 cut(s) 113, 131
Hpy99I CGWCG 4 cut(s) 37, 88, 91, 255
HpyAV CCTTC 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 50, 59
Hsp92I GRCGYC 1 cut(s) 83
HspAI GCGC 1 cut(s) 223
LguI GCTCTTC 1 cut(s) 114
LmnI GCTCC 2 cut(s) 160, 275
LpnPI CCDG 6 cut(s) 34, 105, 119, 143, 228, 246
Lsp1109I GCAGC 1 cut(s) 62
MaeIII GTNAC 1 cut(s) 238
MbiI CCGCTC 1 cut(s) 124
MboII GAAGA 5 cut(s) 28, 77, 130, 131, 158
MluCI AATT 2 cut(s) 134, 173
MlyI GAGTC 2 cut(s) 111, 256
MmeI TCCRAC 2 cut(s) 91, 109
MnlI CCTC 4 cut(s) 22, 162, 190, 221
MseI TTAA 1 cut(s) 257
MspA1I CMGCKG 2 cut(s) 53, 236
MspI CCGG 3 cut(s) 21, 92, 106
MspR9I CCNGG 2 cut(s) 22, 107
MvnI CGCG 1 cut(s) 225
MwoI GCNNNNNNNGC 3 cut(s) 11, 50, 59
NciI CCSGG 2 cut(s) 22, 107
NmuCI GTSAC 1 cut(s) 238
PciSI GCTCTTC 1 cut(s) 114
PfeI GAWTC 1 cut(s) 56
PkrI GCNGC 3 cut(s) 46, 49, 52
PleI GAGTC 2 cut(s) 110, 256
PpsI GAGTC 2 cut(s) 110, 256
PspPI GGNCC 1 cut(s) 230
SapI GCTCTTC 1 cut(s) 114
SaqAI TTAA 1 cut(s) 257
SatI GCNGC 3 cut(s) 45, 48, 51
Sau96I GGNCC 1 cut(s) 230
SchI GAGTC 2 cut(s) 111, 256
ScrFI CCNGG 2 cut(s) 22, 107
SetI ASST 3 cut(s) 159, 165, 232
SinI GGWCC 1 cut(s) 230
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
Sse9I AATT 2 cut(s) 134, 173
SsiI CCGC 8 cut(s) 5, 44, 47, 53, 77, 124, 225, 236
StyD4I CCNGG 2 cut(s) 20, 105
TaqI TCGA 2 cut(s) 207, 253
TasI AATT 2 cut(s) 134, 173
TauI GCSGC 2 cut(s) 47, 50
TfiI GAWTC 1 cut(s) 56
Tru1I TTAA 1 cut(s) 257
Tru9I TTAA 1 cut(s) 257
TseFI GTSAC 1 cut(s) 238
TseI GCWGC 1 cut(s) 50
Tsp45I GTSAC 1 cut(s) 238
VpaK11BI GGWCC 1 cut(s) 230
XapI RAATTY 2 cut(s) 134, 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.