Rh5BG259600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0008161
Physical Location & Seq
Reverse (-)
32482780 .. 32483055
276 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG259600.1

Sequence Viewer

Length: 276 bp
ATGGCAGAGAAGGCGAAGACCGGGAGCGAGGCGACGGGATCGGCGGCGGTAGCGGATTCGGCCGGAGATACTCGATCGGGGGCGTCGTCGCCGGAAGAGGGAGTTCGGGTCGGATGTGAAGAGCGGGTCGGAGAATTCTTCAAGGAAGATGATGAGCTGGAGCTTGAGAGGGAATTCTCGCTTGTTGAGACAGGAGAGGGCTTGGTCGATGATGATTGGGTGCGCGGAGGTCCAGCGGTGGCGGCGTGCACCGCCGTGCTGGAAATCTTAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

91

Amino Acids

9.48

Weight (kDa)

4.09

Isoelectric Point (pI)

39.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 124
AccII CGCG 1 cut(s) 225
AciI CCGC 8 cut(s) 44, 47, 53, 124, 225, 236, 242, 252
AclWI GGATC 1 cut(s) 46
AcoI YGGCCR 1 cut(s) 60
AcsI RAATTY 2 cut(s) 134, 173
AcyI GRCGYC 1 cut(s) 83
AfiI CCNNNNNNNGG 1 cut(s) 98
AgsI TTSAA 1 cut(s) 142
AleI CACNNNNGTG 1 cut(s) 254
AluBI AGCT 2 cut(s) 157, 163
AluI AGCT 2 cut(s) 157, 163
Alw21I GWGCWC 1 cut(s) 251
Alw26I GTCTC 1 cut(s) 182
Alw44I GTGCAC 1 cut(s) 247
AlwI GGATC 1 cut(s) 46
AoxI GGCC 1 cut(s) 60
ApaLI GTGCAC 1 cut(s) 247
ApoI RAATTY 2 cut(s) 134, 173
AspLEI GCGC 1 cut(s) 225
AspS9I GGNCC 1 cut(s) 230
AsuC2I CCSGG 1 cut(s) 22
AvaII GGWCC 1 cut(s) 230
BaeGI GKGCMC 1 cut(s) 251
BbsI GAAGAC 1 cut(s) 23
Bbv12I GWGCWC 1 cut(s) 251
BceAI ACGGC 1 cut(s) 239
BcnI CCSGG 1 cut(s) 22
BcoDI GTCTC 1 cut(s) 182
BisI GCNGC 2 cut(s) 45, 243
BlsI GCNGC 2 cut(s) 46, 244
Bme1390I CCNGG 1 cut(s) 22
Bme18I GGWCC 1 cut(s) 230
BmgT120I GGNCC 1 cut(s) 230
BmrFI CCNGG 1 cut(s) 22
BpiI GAAGAC 1 cut(s) 23
BpmI CTGGAG 1 cut(s) 179
BpuEI CTTGAG 1 cut(s) 185
BpuMI CCSGG 1 cut(s) 22
BsaHI GRCGYC 1 cut(s) 83
BsaXI ACNNNNNCTCC 2 cut(s) 93, 123
Bsc4I CCNNNNNNNGG 1 cut(s) 98
BseGI GGATG 1 cut(s) 119
BseLI CCNNNNNNNGG 1 cut(s) 98
BseSI GKGCMC 1 cut(s) 251
BseX3I CGGCCG 1 cut(s) 60
Bsh1236I CGCG 1 cut(s) 225
Bsh1285I CGRYCG 2 cut(s) 63, 77
BshFI GGCC 1 cut(s) 62
BsiEI CGRYCG 2 cut(s) 63, 77
BsiHKAI GWGCWC 1 cut(s) 251
BsiSI CCGG 3 cut(s) 21, 63, 92
BslI CCNNNNNNNGG 1 cut(s) 98
BsmAI GTCTC 1 cut(s) 182
BsnI GGCC 1 cut(s) 62
Bsp1286I GDGCHC 1 cut(s) 251
Bsp143I GATC 2 cut(s) 38, 74
BspACI CCGC 8 cut(s) 44, 47, 53, 124, 225, 236, 242, 252
BspANI GGCC 1 cut(s) 62
BspFNI CGCG 1 cut(s) 225
BspPI GGATC 1 cut(s) 46
BspQI GCTCTTC 1 cut(s) 114
BsrBI CCGCTC 1 cut(s) 124
BssMI GATC 2 cut(s) 38, 74
BssNI GRCGYC 1 cut(s) 83
Bst6I CTCTTC 2 cut(s) 90, 114
BstACI GRCGYC 1 cut(s) 83
BstC8I GCNNGC 1 cut(s) 247
BstDEI CTNAG 1 cut(s) 268
BstF5I GGATG 1 cut(s) 119
BstFNI CGCG 1 cut(s) 225
BstHHI GCGC 1 cut(s) 225
BstKTI GATC 2 cut(s) 41, 77
BstMAI GTCTC 1 cut(s) 182
BstMBI GATC 2 cut(s) 38, 74
BstMCI CGRYCG 2 cut(s) 63, 77
BstMWI GCNNNNNNNGC 5 cut(s) 11, 50, 59, 242, 251
BstSCI CCNGG 1 cut(s) 20
BstSLI GKGCMC 1 cut(s) 251
BstUI CGCG 1 cut(s) 225
BstV2I GAAGAC 1 cut(s) 23
BstZI CGGCCG 1 cut(s) 60
BsuRI GGCC 1 cut(s) 62
BtsCI GGATG 1 cut(s) 119
Cac8I GCNNGC 1 cut(s) 247
CfoI GCGC 1 cut(s) 225
Cfr13I GGNCC 1 cut(s) 230
CseI GACGC 1 cut(s) 72
CviJI RGCY 4 cut(s) 62, 157, 163, 201
CviKI_1 RGCY 4 cut(s) 62, 157, 163, 201
DdeI CTNAG 1 cut(s) 268
DpnI GATC 2 cut(s) 40, 76
DpnII GATC 2 cut(s) 38, 74
EaeI YGGCCR 1 cut(s) 60
EagI CGGCCG 1 cut(s) 60
Eam1104I CTCTTC 2 cut(s) 90, 114
EarI CTCTTC 2 cut(s) 90, 114
EclXI CGGCCG 1 cut(s) 60
Eco47I GGWCC 1 cut(s) 230
Eco52I CGGCCG 1 cut(s) 60
EcoRI GAATTC 2 cut(s) 134, 173
FauI CCCGC 1 cut(s) 117
Fnu4HI GCNGC 2 cut(s) 45, 243
FokI GGATG 1 cut(s) 126
Fsp4HI GCNGC 2 cut(s) 45, 243
GlaI GCGC 1 cut(s) 224
GluI GCNGC 2 cut(s) 45, 243
GsuI CTGGAG 1 cut(s) 179
HaeIII GGCC 1 cut(s) 62
HapII CCGG 3 cut(s) 21, 63, 92
HgaI GACGC 1 cut(s) 72
HhaI GCGC 1 cut(s) 225
Hin1I GRCGYC 1 cut(s) 83
Hin6I GCGC 1 cut(s) 223
HinP1I GCGC 1 cut(s) 223
HinfI GANTC 1 cut(s) 56
HpaII CCGG 3 cut(s) 21, 63, 92
Hpy166II GTNNAC 1 cut(s) 249
Hpy188I TCNGA 2 cut(s) 113, 131
Hpy8I GTNNAC 1 cut(s) 249
Hpy99I CGWCG 3 cut(s) 37, 88, 91
HpyAV CCTTC 1 cut(s) 4
HpyCH4V TGCA 1 cut(s) 249
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 50, 59, 242, 251
HpyF3I CTNAG 1 cut(s) 268
Hsp92I GRCGYC 1 cut(s) 83
HspAI GCGC 1 cut(s) 223
Kzo9I GATC 2 cut(s) 38, 74
LguI GCTCTTC 1 cut(s) 114
LmnI GCTCC 2 cut(s) 24, 160
LpnPI CCDG 7 cut(s) 34, 76, 105, 143, 177, 245, 246
MalI GATC 2 cut(s) 40, 76
MbiI CCGCTC 1 cut(s) 124
MboI GATC 2 cut(s) 38, 74
MboII GAAGA 5 cut(s) 28, 107, 130, 131, 158
MhlI GDGCHC 1 cut(s) 251
MluCI AATT 2 cut(s) 134, 173
MmeI TCCRAC 2 cut(s) 91, 109
MnlI CCTC 5 cut(s) 22, 91, 162, 190, 221
MseI TTAA 1 cut(s) 274
MslI CAYNNNNRTG 1 cut(s) 254
MspA1I CMGCKG 1 cut(s) 236
MspI CCGG 3 cut(s) 21, 63, 92
MspR9I CCNGG 1 cut(s) 22
MvnI CGCG 1 cut(s) 225
MwoI GCNNNNNNNGC 5 cut(s) 11, 50, 59, 242, 251
NciI CCSGG 1 cut(s) 22
NdeII GATC 2 cut(s) 38, 74
OliI CACNNNNGTG 1 cut(s) 254
PciSI GCTCTTC 1 cut(s) 114
PcsI WCGNNNNNNNCGW 1 cut(s) 83
PfeI GAWTC 1 cut(s) 56
PkrI GCNGC 2 cut(s) 46, 244
Ple19I CGATCG 1 cut(s) 77
PspPI GGNCC 1 cut(s) 230
PvuI CGATCG 1 cut(s) 77
RseI CAYNNNNRTG 1 cut(s) 254
SapI GCTCTTC 1 cut(s) 114
SaqAI TTAA 1 cut(s) 274
SatI GCNGC 2 cut(s) 45, 243
Sau3AI GATC 2 cut(s) 38, 74
Sau96I GGNCC 1 cut(s) 230
ScrFI CCNGG 1 cut(s) 22
SduI GDGCHC 1 cut(s) 251
SetI ASST 3 cut(s) 159, 165, 232
SinI GGWCC 1 cut(s) 230
SmiMI CAYNNNNRTG 1 cut(s) 254
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
Sse9I AATT 2 cut(s) 134, 173
SsiI CCGC 8 cut(s) 44, 47, 53, 124, 225, 236, 242, 252
StyD4I CCNGG 1 cut(s) 20
TaqI TCGA 2 cut(s) 73, 207
TasI AATT 2 cut(s) 134, 173
TauI GCSGC 2 cut(s) 47, 245
TfiI GAWTC 1 cut(s) 56
Tru1I TTAA 1 cut(s) 274
Tru9I TTAA 1 cut(s) 274
VneI GTGCAC 1 cut(s) 247
VpaK11BI GGWCC 1 cut(s) 230
XapI RAATTY 2 cut(s) 134, 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.