Rh4DG254100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0008161
Physical Location & Seq
Forward (+)
46959675 .. 46961250
1576 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG254100.1

Sequence Viewer

Length: 357 bp
ATGAAGAACAAGAAGAGAATTCAGGTCGGATGTGAAGTGCGAGCCGGAGAACTCTTTGAGGACGATGATGAGCTGGATCTTGAGAGGGAATTCACACTTGTTGAGACAAAAGAGAGCTTGGTCGGTGAGGATTGGGTGCGCGGTGATCCACCGGCCAACACAAGCCTCCATACCAATAAAGTTTCGTATAAGTTCAAGAAGTTGAAGACACCATTCGCAACAACAAGTCCGGCCATTGAATCTCCAGGTCATGTTCCTCGTCGTGCTCGTGTGGAGGAGGTGATTGCACCAGATGCTGGTTTGTGGTTGAGAGGTGTGATCGGTGTCGGGAGGATCCTAAACGCTATGGTAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

118

Amino Acids

13.13

Weight (kDa)

6.14

Isoelectric Point (pI)

43.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 296
AccII CGCG 1 cut(s) 141
AciI CCGC 1 cut(s) 141
AclWI GGATC 4 cut(s) 84, 140, 328, 341
AcoI YGGCCR 2 cut(s) 153, 231
AcsI RAATTY 2 cut(s) 18, 89
AfiI CCNNNNNNNGG 1 cut(s) 296
AgsI TTSAA 3 cut(s) 196, 205, 239
AjnI CCWGG 1 cut(s) 244
AluBI AGCT 2 cut(s) 73, 117
AluI AGCT 2 cut(s) 73, 117
Alw21I GWGCWC 1 cut(s) 268
Alw26I GTCTC 1 cut(s) 98
AlwI GGATC 4 cut(s) 84, 140, 328, 341
AlwNI CAGNNNCTG 1 cut(s) 296
AoxI GGCC 2 cut(s) 153, 231
ApoI RAATTY 2 cut(s) 18, 89
AspLEI GCGC 1 cut(s) 141
AsuHPI GGTGA 3 cut(s) 137, 155, 292
BamHI GGATCC 1 cut(s) 333
BauI CACGAG 1 cut(s) 267
BbsI GAAGAC 1 cut(s) 212
Bbv12I GWGCWC 1 cut(s) 268
BciT130I CCWGG 1 cut(s) 246
BcoDI GTCTC 1 cut(s) 98
Bme1390I CCNGG 1 cut(s) 246
BmiI GGNNCC 1 cut(s) 335
BmrFI CCNGG 1 cut(s) 246
BmsI GCATC 1 cut(s) 283
BpiI GAAGAC 1 cut(s) 212
BpmI CTGGAG 1 cut(s) 228
BpuEI CTTGAG 1 cut(s) 101
Bsc4I CCNNNNNNNGG 1 cut(s) 296
Bse118I RCCGGY 1 cut(s) 151
BseBI CCWGG 1 cut(s) 246
BseGI GGATG 1 cut(s) 35
BseLI CCNNNNNNNGG 1 cut(s) 296
BseRI GAGGAG 1 cut(s) 290
Bsh1236I CGCG 1 cut(s) 141
BshFI GGCC 2 cut(s) 155, 233
BsiHKAI GWGCWC 1 cut(s) 268
BsiSI CCGG 3 cut(s) 45, 152, 230
BslI CCNNNNNNNGG 1 cut(s) 296
BsmAI GTCTC 1 cut(s) 98
BsnI GGCC 2 cut(s) 155, 233
Bsp1286I GDGCHC 1 cut(s) 268
Bsp143I GATC 4 cut(s) 76, 145, 318, 333
BspACI CCGC 1 cut(s) 141
BspANI GGCC 2 cut(s) 155, 233
BspFNI CGCG 1 cut(s) 141
BspLI GGNNCC 1 cut(s) 335
BspPI GGATC 4 cut(s) 84, 140, 328, 341
BsrFI RCCGGY 1 cut(s) 151
BssAI RCCGGY 1 cut(s) 151
BssMI GATC 4 cut(s) 76, 145, 318, 333
BssSI CACGAG 1 cut(s) 267
Bst2BI CACGAG 1 cut(s) 267
Bst2UI CCWGG 1 cut(s) 246
Bst6I CTCTTC 1 cut(s) 8
BstAPI GCANNNNNTGC 1 cut(s) 293
BstC8I GCNNGC 1 cut(s) 42
BstF5I GGATG 1 cut(s) 35
BstFNI CGCG 1 cut(s) 141
BstHHI GCGC 1 cut(s) 141
BstKTI GATC 4 cut(s) 79, 148, 321, 336
BstMAI GTCTC 1 cut(s) 98
BstMBI GATC 4 cut(s) 76, 145, 318, 333
BstMWI GCNNNNNNNGC 1 cut(s) 293
BstNI CCWGG 1 cut(s) 246
BstSCI CCNGG 1 cut(s) 244
BstUI CGCG 1 cut(s) 141
BstV2I GAAGAC 1 cut(s) 212
BstX2I RGATCY 2 cut(s) 76, 333
BstYI RGATCY 2 cut(s) 76, 333
BsuRI GGCC 2 cut(s) 155, 233
BtsCI GGATG 1 cut(s) 35
Cac8I GCNNGC 1 cut(s) 42
CaiI CAGNNNCTG 1 cut(s) 296
CfoI GCGC 1 cut(s) 141
Cfr10I RCCGGY 1 cut(s) 151
CviAII CATG 1 cut(s) 251
CviJI RGCY 6 cut(s) 44, 73, 117, 155, 165, 233
CviKI_1 RGCY 6 cut(s) 44, 73, 117, 155, 165, 233
DpnI GATC 4 cut(s) 78, 147, 320, 335
DpnII GATC 4 cut(s) 76, 145, 318, 333
EaeI YGGCCR 2 cut(s) 153, 231
Eam1104I CTCTTC 1 cut(s) 8
EarI CTCTTC 1 cut(s) 8
EcoRI GAATTC 2 cut(s) 18, 89
EcoRII CCWGG 1 cut(s) 244
FaeI CATG 1 cut(s) 254
FaiI YATR 4 cut(s) 171, 189, 252, 347
FatI CATG 1 cut(s) 250
FokI GGATG 1 cut(s) 42
GlaI GCGC 1 cut(s) 140
GsuI CTGGAG 1 cut(s) 228
HaeIII GGCC 2 cut(s) 155, 233
HapII CCGG 3 cut(s) 45, 152, 230
HhaI GCGC 1 cut(s) 141
Hin1II CATG 1 cut(s) 254
Hin6I GCGC 1 cut(s) 139
HinP1I GCGC 1 cut(s) 139
HinfI GANTC 1 cut(s) 239
HpaII CCGG 3 cut(s) 45, 152, 230
HphI GGTGA 3 cut(s) 137, 155, 292
Hpy188I TCNGA 1 cut(s) 29
Hpy188III TCNNGA 3 cut(s) 80, 196, 328
Hpy99I CGWCG 1 cut(s) 264
HpyCH4V TGCA 1 cut(s) 287
HpyF10VI GCNNNNNNNGC 1 cut(s) 293
Hsp92II CATG 1 cut(s) 254
HspAI GCGC 1 cut(s) 139
Kzo9I GATC 4 cut(s) 76, 145, 318, 333
LpnPI CCDG 9 cut(s) 8, 58, 59, 165, 231, 243, 258, 282, 303
LweI GCATC 1 cut(s) 283
MalI GATC 4 cut(s) 78, 147, 320, 335
MboI GATC 4 cut(s) 76, 145, 318, 333
MboII GAAGA 3 cut(s) 16, 25, 217
MflI RGATCY 2 cut(s) 76, 333
MhlI GDGCHC 1 cut(s) 268
MluCI AATT 2 cut(s) 18, 89
MmeI TCCRAC 1 cut(s) 7
MnlI CCTC 9 cut(s) 52, 78, 121, 176, 267, 268, 271, 305, 324
MspI CCGG 3 cut(s) 45, 152, 230
MspR9I CCNGG 1 cut(s) 246
MvaI CCWGG 1 cut(s) 246
MvnI CGCG 1 cut(s) 141
MwoI GCNNNNNNNGC 1 cut(s) 293
NdeII GATC 4 cut(s) 76, 145, 318, 333
NlaIII CATG 1 cut(s) 254
NlaIV GGNNCC 1 cut(s) 335
PcsI WCGNNNNNNNCGW 1 cut(s) 265
PfeI GAWTC 1 cut(s) 239
PflMI CCANNNNNTGG 1 cut(s) 296
Psp6I CCWGG 1 cut(s) 244
PspGI CCWGG 1 cut(s) 244
PspN4I GGNNCC 1 cut(s) 335
PstNI CAGNNNCTG 1 cut(s) 296
PsuI RGATCY 2 cut(s) 76, 333
Sau3AI GATC 4 cut(s) 76, 145, 318, 333
ScrFI CCNGG 1 cut(s) 246
SduI GDGCHC 1 cut(s) 268
SetI ASST 6 cut(s) 27, 75, 119, 250, 282, 316
SfaNI GCATC 1 cut(s) 283
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
Sse9I AATT 2 cut(s) 18, 89
SsiI CCGC 1 cut(s) 141
StyD4I CCNGG 1 cut(s) 244
TasI AATT 2 cut(s) 18, 89
TfiI GAWTC 1 cut(s) 239
TspDTI ATGAA 1 cut(s) 17
Van91I CCANNNNNTGG 1 cut(s) 296
XapI RAATTY 2 cut(s) 18, 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.