Rh2DG539700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0008161
Physical Location & Seq
Reverse (-)
76868704 .. 76868979
276 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG539700.1

Sequence Viewer

Length: 276 bp
ATGGCGGAGAAGGCGAAGACCGGGATCAAGGTGACGGGATCGGCTGCGGCAGTGGATTCGGCCGGAGATACTCGAGCGGGGGCGTCGTCGCTGGAAGAGGGAGTCCGGGTCGGATGTGAAGAGCTGGTCGGAGAATTCTTCAAGGAAGACGATGAGCTGGAGCTTGAGAGGGAATTCTCACTTGTTGAGACAAGAGAGGGCTTGGTCGATGATGATTGGGTGCCCGAAGGTCCAGCGGTGGCGACGTGCGCCGCCGTGCTGGAAATCGTCGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

91

Amino Acids

9.59

Weight (kDa)

4.09

Isoelectric Point (pI)

13.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 220
AccBSI CCGCTC 1 cut(s) 77
AciI CCGC 5 cut(s) 5, 47, 77, 236, 252
AclWI GGATC 2 cut(s) 32, 46
AcoI YGGCCR 1 cut(s) 60
AcsI RAATTY 2 cut(s) 134, 173
AcyI GRCGYC 1 cut(s) 83
AgsI TTSAA 1 cut(s) 142
AjiI CACGTC 1 cut(s) 246
AluBI AGCT 3 cut(s) 124, 157, 163
AluI AGCT 3 cut(s) 124, 157, 163
Alw26I GTCTC 1 cut(s) 182
AlwI GGATC 2 cut(s) 32, 46
Ama87I CYCGRG 1 cut(s) 72
AoxI GGCC 1 cut(s) 60
ApeKI GCWGC 1 cut(s) 44
ApoI RAATTY 2 cut(s) 134, 173
AspLEI GCGC 1 cut(s) 251
AspS9I GGNCC 1 cut(s) 230
AsuC2I CCSGG 2 cut(s) 22, 107
AsuHPI GGTGA 1 cut(s) 43
AvaI CYCGRG 1 cut(s) 72
AvaII GGWCC 1 cut(s) 230
BaeGI GKGCMC 1 cut(s) 225
BanI GGYRCC 1 cut(s) 220
BbsI GAAGAC 2 cut(s) 23, 153
BbvI GCAGC 1 cut(s) 31
BceAI ACGGC 1 cut(s) 239
BcgI CGANNNNNNTGC 2 cut(s) 39, 73
BcnI CCSGG 2 cut(s) 22, 107
BcoDI GTCTC 1 cut(s) 182
BisI GCNGC 3 cut(s) 45, 48, 252
BlsI GCNGC 3 cut(s) 46, 49, 253
Bme1390I CCNGG 2 cut(s) 22, 107
Bme18I GGWCC 1 cut(s) 230
BmeT110I CYCGRG 1 cut(s) 72
BmgBI CACGTC 1 cut(s) 246
BmgT120I GGNCC 1 cut(s) 230
BmiI GGNNCC 1 cut(s) 222
BmrFI CCNGG 2 cut(s) 22, 107
BpiI GAAGAC 2 cut(s) 23, 153
BpmI CTGGAG 1 cut(s) 179
BpuEI CTTGAG 1 cut(s) 185
BpuMI CCSGG 2 cut(s) 22, 107
BsaHI GRCGYC 1 cut(s) 83
BsaXI ACNNNNNCTCC 2 cut(s) 93, 123
BseGI GGATG 1 cut(s) 119
BseSI GKGCMC 1 cut(s) 225
BseX3I CGGCCG 1 cut(s) 60
BseXI GCAGC 1 cut(s) 31
Bsh1285I CGRYCG 1 cut(s) 63
BshFI GGCC 1 cut(s) 62
BshNI GGYRCC 1 cut(s) 220
BsiEI CGRYCG 1 cut(s) 63
BsiHKCI CYCGRG 1 cut(s) 72
BsiSI CCGG 3 cut(s) 21, 63, 106
BsmAI GTCTC 1 cut(s) 182
BsnI GGCC 1 cut(s) 62
BsoBI CYCGRG 1 cut(s) 72
Bsp1286I GDGCHC 1 cut(s) 225
Bsp143I GATC 2 cut(s) 24, 38
BspACI CCGC 5 cut(s) 5, 47, 77, 236, 252
BspANI GGCC 1 cut(s) 62
BspLI GGNNCC 1 cut(s) 222
BspPI GGATC 2 cut(s) 32, 46
BspQI GCTCTTC 1 cut(s) 114
BspT107I GGYRCC 1 cut(s) 220
BsrBI CCGCTC 1 cut(s) 77
BssMI GATC 2 cut(s) 24, 38
BssNI GRCGYC 1 cut(s) 83
Bst6I CTCTTC 2 cut(s) 90, 114
BstACI GRCGYC 1 cut(s) 83
BstF5I GGATG 1 cut(s) 119
BstHHI GCGC 1 cut(s) 251
BstKTI GATC 2 cut(s) 27, 41
BstMAI GTCTC 1 cut(s) 182
BstMBI GATC 2 cut(s) 24, 38
BstMCI CGRYCG 1 cut(s) 63
BstMWI GCNNNNNNNGC 2 cut(s) 11, 248
BstSCI CCNGG 2 cut(s) 20, 105
BstSLI GKGCMC 1 cut(s) 225
BstV1I GCAGC 1 cut(s) 31
BstV2I GAAGAC 2 cut(s) 23, 153
BstZI CGGCCG 1 cut(s) 60
BsuRI GGCC 1 cut(s) 62
BtrI CACGTC 1 cut(s) 246
BtsCI GGATG 1 cut(s) 119
BtsI GCAGTG 1 cut(s) 57
BtsIMutI CAGTG 1 cut(s) 57
CfoI GCGC 1 cut(s) 251
Cfr13I GGNCC 1 cut(s) 230
CseI GACGC 1 cut(s) 72
CviJI RGCY 6 cut(s) 44, 62, 124, 157, 163, 201
CviKI_1 RGCY 6 cut(s) 44, 62, 124, 157, 163, 201
DpnI GATC 2 cut(s) 26, 40
DpnII GATC 2 cut(s) 24, 38
EaeI YGGCCR 1 cut(s) 60
EagI CGGCCG 1 cut(s) 60
Eam1104I CTCTTC 2 cut(s) 90, 114
EarI CTCTTC 2 cut(s) 90, 114
EciI GGCGGA 1 cut(s) 20
EclXI CGGCCG 1 cut(s) 60
Eco47I GGWCC 1 cut(s) 230
Eco52I CGGCCG 1 cut(s) 60
Eco88I CYCGRG 1 cut(s) 72
EcoRI GAATTC 2 cut(s) 134, 173
FauI CCCGC 1 cut(s) 70
Fnu4HI GCNGC 3 cut(s) 45, 48, 252
FokI GGATG 1 cut(s) 126
Fsp4HI GCNGC 3 cut(s) 45, 48, 252
GlaI GCGC 1 cut(s) 250
GluI GCNGC 3 cut(s) 45, 48, 252
GsuI CTGGAG 1 cut(s) 179
HaeIII GGCC 1 cut(s) 62
HapII CCGG 3 cut(s) 21, 63, 106
HgaI GACGC 1 cut(s) 72
HhaI GCGC 1 cut(s) 251
Hin1I GRCGYC 1 cut(s) 83
Hin6I GCGC 1 cut(s) 249
HinP1I GCGC 1 cut(s) 249
HinfI GANTC 2 cut(s) 56, 102
HpaII CCGG 3 cut(s) 21, 63, 106
HphI GGTGA 1 cut(s) 43
Hpy188I TCNGA 2 cut(s) 113, 131
Hpy99I CGWCG 4 cut(s) 88, 91, 247, 272
HpyAV CCTTC 2 cut(s) 4, 221
HpyCH4IV ACGT 1 cut(s) 245
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 248
HpySE526I ACGT 1 cut(s) 245
Hsp92I GRCGYC 1 cut(s) 83
HspAI GCGC 1 cut(s) 249
Kzo9I GATC 2 cut(s) 24, 38
LguI GCTCTTC 1 cut(s) 114
LmnI GCTCC 1 cut(s) 160
LpnPI CCDG 8 cut(s) 34, 76, 77, 110, 119, 143, 245, 246
Lsp1109I GCAGC 1 cut(s) 31
MaeII ACGT 1 cut(s) 245
MaeIII GTNAC 1 cut(s) 31
MalI GATC 2 cut(s) 26, 40
MbiI CCGCTC 1 cut(s) 77
MboI GATC 2 cut(s) 24, 38
MboII GAAGA 5 cut(s) 28, 107, 130, 131, 158
MhlI GDGCHC 1 cut(s) 225
MluCI AATT 2 cut(s) 134, 173
MlyI GAGTC 1 cut(s) 111
MmeI TCCRAC 2 cut(s) 91, 109
MnlI CCTC 3 cut(s) 91, 162, 190
MseI TTAA 1 cut(s) 274
MspA1I CMGCKG 1 cut(s) 236
MspI CCGG 3 cut(s) 21, 63, 106
MspR9I CCNGG 2 cut(s) 22, 107
MwoI GCNNNNNNNGC 2 cut(s) 11, 248
NciI CCSGG 2 cut(s) 22, 107
NdeII GATC 2 cut(s) 24, 38
NlaIV GGNNCC 1 cut(s) 222
NmuCI GTSAC 1 cut(s) 31
PaeR7I CTCGAG 1 cut(s) 72
PciSI GCTCTTC 1 cut(s) 114
PfeI GAWTC 1 cut(s) 56
PkrI GCNGC 3 cut(s) 46, 49, 253
PleI GAGTC 1 cut(s) 110
PpsI GAGTC 1 cut(s) 110
PspN4I GGNNCC 1 cut(s) 222
PspPI GGNCC 1 cut(s) 230
PspXI VCTCGAGB 1 cut(s) 72
SapI GCTCTTC 1 cut(s) 114
SaqAI TTAA 1 cut(s) 274
SatI GCNGC 3 cut(s) 45, 48, 252
Sau3AI GATC 2 cut(s) 24, 38
Sau96I GGNCC 1 cut(s) 230
SchI GAGTC 1 cut(s) 111
ScrFI CCNGG 2 cut(s) 22, 107
SduI GDGCHC 1 cut(s) 225
SetI ASST 6 cut(s) 33, 126, 159, 165, 232, 248
Sfr274I CTCGAG 1 cut(s) 72
SinI GGWCC 1 cut(s) 230
SlaI CTCGAG 1 cut(s) 72
SmlI CTYRAG 2 cut(s) 72, 164
SmoI CTYRAG 2 cut(s) 72, 164
Sse9I AATT 2 cut(s) 134, 173
SsiI CCGC 5 cut(s) 5, 47, 77, 236, 252
StyD4I CCNGG 2 cut(s) 20, 105
TaiI ACGT 1 cut(s) 248
TaqI TCGA 3 cut(s) 73, 207, 270
TasI AATT 2 cut(s) 134, 173
TauI GCSGC 2 cut(s) 50, 254
TfiI GAWTC 1 cut(s) 56
Tru1I TTAA 1 cut(s) 274
Tru9I TTAA 1 cut(s) 274
TscAI CASTG 1 cut(s) 57
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 1 cut(s) 44
Tsp45I GTSAC 1 cut(s) 31
TspRI CASTG 1 cut(s) 57
VpaK11BI GGWCC 1 cut(s) 230
XapI RAATTY 2 cut(s) 134, 173
XhoI CTCGAG 1 cut(s) 72
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.