pycom02g20070

Glycine-rich protein A3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0002276
Physical Location & Seq
Forward (+)
18200939 .. 18202083
1145 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g20070.1

Sequence Viewer

Length: 537 bp
ATGGGAGGGGGAACAGGAAACAACGACGAGTCTACTGACAGAGGGCTATTTTCAAATCTTGCTGGATTTGCTTCTGGAAACCTTGCACGACCTGGATCACACCATGGATATCCCCAAGGTGGGTACCCTCCTCAAGGCGGTTACCCTCCCCAAGGTGGATACCCTCCTTCCGGTGGGTACCCTCCATCTGGGTATCCTCCCGGACCTCCCCCCGCAGGATATCCACCACCAGCCCATCCGGCCCCTGGTGGATATCCACCAGCCGGTTATCCAGGTCCATCAGCTCCACATCATTCAGGACGTGGATCATCAGGTATGGGGATCGGGGCCATGTTAGCTTGGGGTACTGCAGCCGCAGCTGCTGGCTACGGAGTTCACCATCTTGTGCATGGGAGTCACCATGGTGGCGGATACGGTCATGGCGGTGGATACGGTCATGGTGGCTATGGGGGCTTCGGTTATGGAAAGTTCAAACATGGGAAACATGGAAAGTTTAAGCATGGGAAACATGGCAAGTTCAGGAAATGGAAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

179

Amino Acids

17.79

Weight (kDa)

9.81

Isoelectric Point (pI)

29.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 123, 177
AccB1I GGYRCC 2 cut(s) 123, 177
AccI GTMKAC 1 cut(s) 32
AciI CCGC 5 cut(s) 138, 213, 354, 408, 423
AclWI GGATC 3 cut(s) 103, 313, 329
AfaI GTAC 3 cut(s) 125, 179, 346
AgsI TTSAA 2 cut(s) 54, 472
AjiI CACGTC 1 cut(s) 302
AjnI CCWGG 3 cut(s) 91, 244, 271
AleI CACNNNNGTG 1 cut(s) 402
AluBI AGCT 3 cut(s) 284, 338, 359
AluI AGCT 3 cut(s) 284, 338, 359
AlwI GGATC 3 cut(s) 103, 313, 329
AlwNI CAGNNNCTG 1 cut(s) 362
AoxI GGCC 2 cut(s) 240, 327
ApeKI GCWGC 3 cut(s) 350, 356, 359
Asp718I GGTACC 2 cut(s) 123, 177
AspS9I GGNCC 4 cut(s) 203, 241, 275, 327
AsuC2I CCSGG 1 cut(s) 201
AsuHPI GGTGA 2 cut(s) 368, 389
AvaII GGWCC 2 cut(s) 203, 275
BanI GGYRCC 2 cut(s) 123, 177
BbvI GCAGC 3 cut(s) 346, 362, 368
BccI CCATC 4 cut(s) 193, 243, 286, 387
BciT130I CCWGG 3 cut(s) 93, 246, 273
BciVI GTATCC 4 cut(s) 152, 204, 404, 422
BcnI CCSGG 1 cut(s) 201
BfmI CTRYAG 1 cut(s) 348
BfuI GTATCC 4 cut(s) 152, 204, 404, 422
BglI GCCNNNNNGGC 1 cut(s) 239
BisI GCNGC 4 cut(s) 351, 354, 357, 360
BlsI GCNGC 4 cut(s) 352, 355, 358, 361
Bme1390I CCNGG 4 cut(s) 93, 201, 246, 273
Bme18I GGWCC 2 cut(s) 203, 275
BmgBI CACGTC 1 cut(s) 302
BmgT120I GGNCC 4 cut(s) 203, 241, 275, 327
BmiI GGNNCC 4 cut(s) 125, 179, 243, 328
BmrFI CCNGG 4 cut(s) 93, 201, 246, 273
BpuEI CTTGAG 1 cut(s) 117
BpuMI CCSGG 1 cut(s) 201
BsaJI CCNNGG 5 cut(s) 103, 115, 151, 244, 400
BsaWI WCCGGW 1 cut(s) 170
Bse118I RCCGGY 1 cut(s) 263
BseBI CCWGG 3 cut(s) 93, 246, 273
BseDI CCNNGG 5 cut(s) 103, 115, 151, 244, 400
BseGI GGATG 1 cut(s) 235
BseRI GAGGAG 1 cut(s) 120
BseXI GCAGC 3 cut(s) 346, 362, 368
BshFI GGCC 2 cut(s) 242, 329
BshNI GGYRCC 2 cut(s) 123, 177
BsiSI CCGG 4 cut(s) 171, 201, 239, 264
BsnI GGCC 2 cut(s) 242, 329
Bsp143I GATC 3 cut(s) 95, 305, 321
Bsp19I CCATGG 2 cut(s) 103, 400
BspACI CCGC 5 cut(s) 138, 213, 354, 408, 423
BspANI GGCC 2 cut(s) 242, 329
BspLI GGNNCC 4 cut(s) 125, 179, 243, 328
BspMAI CTGCAG 1 cut(s) 352
BspPI GGATC 3 cut(s) 103, 313, 329
BspT107I GGYRCC 2 cut(s) 123, 177
BsrFI RCCGGY 1 cut(s) 263
BssAI RCCGGY 1 cut(s) 263
BssECI CCNNGG 5 cut(s) 103, 115, 151, 244, 400
BssMI GATC 3 cut(s) 95, 305, 321
BssT1I CCWWGG 4 cut(s) 103, 115, 151, 400
Bst2UI CCWGG 3 cut(s) 93, 246, 273
Bst4CI ACNGT 2 cut(s) 416, 434
BstC8I GCNNGC 1 cut(s) 364
BstDSI CCRYGG 2 cut(s) 103, 400
BstEII GGTNACC 1 cut(s) 140
BstENI CCTNNNNNAGG 2 cut(s) 132, 150
BstF5I GGATG 1 cut(s) 235
BstKTI GATC 3 cut(s) 98, 308, 324
BstMBI GATC 3 cut(s) 95, 305, 321
BstMWI GCNNNNNNNGC 6 cut(s) 68, 239, 335, 356, 359, 450
BstNI CCWGG 3 cut(s) 93, 246, 273
BstPI GGTNACC 1 cut(s) 140
BstSCI CCNGG 4 cut(s) 91, 199, 244, 271
BstSFI CTRYAG 1 cut(s) 348
BstV1I GCAGC 3 cut(s) 346, 362, 368
BsuI GTATCC 4 cut(s) 152, 204, 404, 422
BsuRI GGCC 2 cut(s) 242, 329
BtgI CCRYGG 2 cut(s) 103, 400
BtrI CACGTC 1 cut(s) 302
BtsCI GGATG 1 cut(s) 235
Cac8I GCNNGC 1 cut(s) 364
CaiI CAGNNNCTG 1 cut(s) 362
Cfr10I RCCGGY 1 cut(s) 263
Cfr13I GGNCC 4 cut(s) 203, 241, 275, 327
Csp6I GTAC 3 cut(s) 124, 178, 345
CviQI GTAC 3 cut(s) 124, 178, 345
DpnI GATC 3 cut(s) 97, 307, 323
DpnII GATC 3 cut(s) 95, 305, 321
EciI GGCGGA 1 cut(s) 423
Eco130I CCWWGG 4 cut(s) 103, 115, 151, 400
Eco32I GATATC 3 cut(s) 110, 221, 254
Eco47I GGWCC 2 cut(s) 203, 275
Eco91I GGTNACC 1 cut(s) 140
EcoNI CCTNNNNNAGG 2 cut(s) 132, 150
EcoO65I GGTNACC 1 cut(s) 140
EcoRII CCWGG 3 cut(s) 91, 244, 271
EcoRV GATATC 3 cut(s) 110, 221, 254
EcoT14I CCWWGG 4 cut(s) 103, 115, 151, 400
ErhI CCWWGG 4 cut(s) 103, 115, 151, 400
FauI CCCGC 1 cut(s) 220
FblI GTMKAC 1 cut(s) 32
Fnu4HI GCNGC 4 cut(s) 351, 354, 357, 360
FokI GGATG 1 cut(s) 222
Fsp4HI GCNGC 4 cut(s) 351, 354, 357, 360
GluI GCNGC 4 cut(s) 351, 354, 357, 360
HaeIII GGCC 2 cut(s) 242, 329
HapII CCGG 4 cut(s) 171, 201, 239, 264
HinfI GANTC 2 cut(s) 29, 394
HpaII CCGG 4 cut(s) 171, 201, 239, 264
HphI GGTGA 2 cut(s) 368, 389
Hpy166II GTNNAC 2 cut(s) 33, 376
Hpy188III TCNNGA 3 cut(s) 75, 297, 520
Hpy8I GTNNAC 2 cut(s) 33, 376
Hpy99I CGWCG 1 cut(s) 29
HpyAV CCTTC 1 cut(s) 177
HpyCH4III ACNGT 2 cut(s) 416, 434
HpyCH4IV ACGT 1 cut(s) 301
HpyCH4V TGCA 3 cut(s) 86, 350, 388
HpyF10VI GCNNNNNNNGC 6 cut(s) 68, 239, 335, 356, 359, 450
HpySE526I ACGT 1 cut(s) 301
KpnI GGTACC 2 cut(s) 127, 181
Kzo9I GATC 3 cut(s) 95, 305, 321
LmnI GCTCC 1 cut(s) 289
Lsp1109I GCAGC 3 cut(s) 346, 362, 368
MaeII ACGT 1 cut(s) 301
MaeIII GTNAC 2 cut(s) 140, 395
MalI GATC 3 cut(s) 97, 307, 323
MboI GATC 3 cut(s) 95, 305, 321
MlyI GAGTC 2 cut(s) 38, 403
MnlI CCTC 8 cut(s) 35, 138, 141, 156, 174, 192, 207, 216
MseI TTAA 1 cut(s) 495
MslI CAYNNNNRTG 2 cut(s) 402, 423
MspA1I CMGCKG 1 cut(s) 359
MspI CCGG 4 cut(s) 171, 201, 239, 264
MspR9I CCNGG 4 cut(s) 93, 201, 246, 273
MvaI CCWGG 3 cut(s) 93, 246, 273
MwoI GCNNNNNNNGC 6 cut(s) 68, 239, 335, 356, 359, 450
NciI CCSGG 1 cut(s) 201
NcoI CCATGG 2 cut(s) 103, 400
NdeII GATC 3 cut(s) 95, 305, 321
NlaIV GGNNCC 4 cut(s) 125, 179, 243, 328
NmuCI GTSAC 1 cut(s) 395
OliI CACNNNNGTG 1 cut(s) 402
PfoI TCCNGGA 1 cut(s) 199
PkrI GCNGC 4 cut(s) 352, 355, 358, 361
PleI GAGTC 2 cut(s) 37, 402
PpsI GAGTC 2 cut(s) 37, 402
Psp6I CCWGG 3 cut(s) 91, 244, 271
PspEI GGTNACC 1 cut(s) 140
PspGI CCWGG 3 cut(s) 91, 244, 271
PspN4I GGNNCC 4 cut(s) 125, 179, 243, 328
PspPI GGNCC 4 cut(s) 203, 241, 275, 327
PstI CTGCAG 1 cut(s) 352
PstNI CAGNNNCTG 1 cut(s) 362
PvuII CAGCTG 1 cut(s) 359
RsaI GTAC 3 cut(s) 125, 179, 346
RsaNI GTAC 3 cut(s) 124, 178, 345
RseI CAYNNNNRTG 2 cut(s) 402, 423
SaqAI TTAA 1 cut(s) 495
SatI GCNGC 4 cut(s) 351, 354, 357, 360
Sau3AI GATC 3 cut(s) 95, 305, 321
Sau96I GGNCC 4 cut(s) 203, 241, 275, 327
SchI GAGTC 2 cut(s) 38, 403
ScrFI CCNGG 4 cut(s) 93, 201, 246, 273
SfcI CTRYAG 1 cut(s) 348
SinI GGWCC 2 cut(s) 203, 275
SmiMI CAYNNNNRTG 2 cut(s) 402, 423
SmlI CTYRAG 1 cut(s) 132
SmoI CTYRAG 1 cut(s) 132
SsiI CCGC 5 cut(s) 138, 213, 354, 408, 423
StyD4I CCNGG 4 cut(s) 91, 199, 244, 271
StyI CCWWGG 4 cut(s) 103, 115, 151, 400
TaaI ACNGT 2 cut(s) 416, 434
TaiI ACGT 1 cut(s) 304
TauI GCSGC 1 cut(s) 356
Tru1I TTAA 1 cut(s) 495
Tru9I TTAA 1 cut(s) 495
TseFI GTSAC 1 cut(s) 395
TseI GCWGC 3 cut(s) 350, 356, 359
Tsp45I GTSAC 1 cut(s) 395
TspGWI ACGGA 1 cut(s) 384
VpaK11BI GGWCC 2 cut(s) 203, 275
XagI CCTNNNNNAGG 2 cut(s) 132, 150
XcmI CCANNNNNNNNNTGG 2 cut(s) 242, 386
XmiI GTMKAC 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.