pycom03g08050

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0003325
Physical Location & Seq
Forward (+)
6467395 .. 6468445
1051 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g08050.1

Sequence Viewer

Length: 630 bp
ATGCGATTTAACATGGGTGAGGAGATCGATCATGTAAAGGATGACACTAAACATGAACAGGCTGGCGGTGCCGGTACTGGTGGCGGCGCCGGCGGCGCTGGCGGTGTCGGCGGTGCTGGCGGTGCCGGGGGTGCTGGCGGTGCCGGTGGCGTCGGAGGTGCCGGCGAGATCCGATTTAACATGGGTGAGGAGAAAGATCATGTAAGGATGACACTAAACATGAACAGGCTGGCGATGCCGGTACTGGTGGCGGTGGCAGTGGCGGTGTTGGCGGTGCCGGTGGCGCTGGAGGTGCCGGCGGTGGTGGCGGCGGCGGCAGTGCCACTGCCGATGGACATCCTACTCTTAATTAGAGTGCCAACAAATAAGCCATCTGAGGGATTCAACGACGTTGGCAACTACTACCGAATGAGGGGCAATTTGCTAGAAGCAATCAAGCTGTGGGATGTTATGCTAGATAGGAGTCTGAAACCTGATATTTTAGCATACAAATTTTTTTATATATGGTTGTTGTGTGCTGGAGAACCAACCAAGGCTTTCGAGTTGCGTGATGACATGATGAGAAGAGGCTTGAAGCCAAATCGAGTTTCATATAACACACTCATCCGAGGGACTTGCCTTACAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

210

Amino Acids

22.06

Weight (kDa)

6.84

Isoelectric Point (pI)

31.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 173 - 206 4.5e-09 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 7 cut(s) 68, 86, 122, 140, 158, 274, 292
AclWI GGATC 1 cut(s) 163
AcsI RAATTY 1 cut(s) 491
AcyI GRCGYC 2 cut(s) 87, 150
AfaI GTAC 2 cut(s) 76, 243
AfiI CCNNNNNNNGG 2 cut(s) 377, 412
AgsI TTSAA 2 cut(s) 385, 574
AjuI GAANNNNNNNTTGG 2 cut(s) 571, 603
AluBI AGCT 1 cut(s) 439
AluI AGCT 1 cut(s) 439
AlwI GGATC 1 cut(s) 163
ApoI RAATTY 1 cut(s) 491
AspLEI GCGC 3 cut(s) 89, 98, 286
AsuC2I CCSGG 1 cut(s) 127
AsuHPI GGTGA 2 cut(s) 29, 197
BanI GGYRCC 7 cut(s) 68, 86, 122, 140, 158, 274, 292
BccI CCATC 2 cut(s) 325, 379
BcgI CGANNNNNNTGC 1 cut(s) 597
BcnI CCSGG 1 cut(s) 127
BfaI CTAG 2 cut(s) 425, 455
BfoI RGCGCY 3 cut(s) 90, 99, 287
BisI GCNGC 5 cut(s) 85, 94, 309, 312, 315
BlsI GCNGC 5 cut(s) 86, 95, 310, 313, 316
Bme1390I CCNGG 1 cut(s) 127
BmiI GGNNCC 7 cut(s) 70, 88, 124, 142, 160, 276, 294
BmrFI CCNGG 1 cut(s) 127
BmsI GCATC 1 cut(s) 225
BpmI CTGGAG 2 cut(s) 308, 540
BpuMI CCSGG 1 cut(s) 127
Bsa29I ATCGAT 1 cut(s) 27
BsaBI GATNNNNATC 1 cut(s) 335
BsaHI GRCGYC 2 cut(s) 87, 150
BsaJI CCNNGG 3 cut(s) 126, 531, 607
Bsc4I CCNNNNNNNGG 2 cut(s) 377, 412
Bse118I RCCGGY 7 cut(s) 71, 89, 143, 161, 238, 277, 295
Bse1I ACTGG 2 cut(s) 82, 249
Bse8I GATNNNNATC 1 cut(s) 335
BseCI ATCGAT 1 cut(s) 27
BseDI CCNNGG 3 cut(s) 126, 531, 607
BseGI GGATG 5 cut(s) 46, 213, 336, 451, 603
BseJI GATNNNNATC 1 cut(s) 335
BseLI CCNNNNNNNGG 2 cut(s) 377, 412
BseMII CTCAG 1 cut(s) 366
BseNI ACTGG 2 cut(s) 82, 249
BseRI GAGGAG 2 cut(s) 35, 203
BshNI GGYRCC 7 cut(s) 68, 86, 122, 140, 158, 274, 292
BshVI ATCGAT 1 cut(s) 27
BsiSI CCGG 8 cut(s) 72, 90, 126, 144, 162, 239, 278, 296
BslFI GGGAC 1 cut(s) 625
BslI CCNNNNNNNGG 2 cut(s) 377, 412
BsmFI GGGAC 1 cut(s) 625
Bsp143I GATC 4 cut(s) 24, 28, 168, 196
BspCNI CTCAG 1 cut(s) 367
BspDI ATCGAT 1 cut(s) 27
BspLI GGNNCC 7 cut(s) 70, 88, 124, 142, 160, 276, 294
BspPI GGATC 1 cut(s) 163
BspT107I GGYRCC 7 cut(s) 68, 86, 122, 140, 158, 274, 292
BsrFI RCCGGY 7 cut(s) 71, 89, 143, 161, 238, 277, 295
BsrI ACTGG 2 cut(s) 82, 249
BssAI RCCGGY 7 cut(s) 71, 89, 143, 161, 238, 277, 295
BssECI CCNNGG 3 cut(s) 126, 531, 607
BssMI GATC 4 cut(s) 24, 28, 168, 196
BssNI GRCGYC 2 cut(s) 87, 150
BssT1I CCWWGG 1 cut(s) 531
Bst6I CTCTTC 1 cut(s) 559
BstACI GRCGYC 2 cut(s) 87, 150
BstC8I GCNNGC 8 cut(s) 64, 91, 100, 118, 136, 163, 231, 297
BstDEI CTNAG 1 cut(s) 375
BstF5I GGATG 5 cut(s) 46, 213, 336, 451, 603
BstH2I RGCGCY 3 cut(s) 90, 99, 287
BstHHI GCGC 3 cut(s) 89, 98, 286
BstKTI GATC 4 cut(s) 27, 31, 171, 199
BstMBI GATC 4 cut(s) 24, 28, 168, 196
BstSCI CCNGG 1 cut(s) 125
BstX2I RGATCY 1 cut(s) 168
BstYI RGATCY 1 cut(s) 168
Bsu15I ATCGAT 1 cut(s) 27
BsuTUI ATCGAT 1 cut(s) 27
BtgZI GCGATG 1 cut(s) 248
BtsCI GGATG 5 cut(s) 46, 213, 336, 451, 603
BtsI GCAGTG 3 cut(s) 264, 323, 324
BtsIMutI CAGTG 3 cut(s) 264, 323, 324
Cac8I GCNNGC 8 cut(s) 64, 91, 100, 118, 136, 163, 231, 297
CfoI GCGC 3 cut(s) 89, 98, 286
Cfr10I RCCGGY 7 cut(s) 71, 89, 143, 161, 238, 277, 295
ClaI ATCGAT 1 cut(s) 27
CseI GACGC 1 cut(s) 139
Csp6I GTAC 2 cut(s) 75, 242
CviAII CATG 7 cut(s) 13, 32, 53, 181, 200, 220, 556
CviJI RGCY 7 cut(s) 62, 229, 370, 439, 536, 570, 577
CviKI_1 RGCY 7 cut(s) 62, 229, 370, 439, 536, 570, 577
CviQI GTAC 2 cut(s) 75, 242
DdeI CTNAG 1 cut(s) 375
DinI GGCGCC 1 cut(s) 88
DpnI GATC 4 cut(s) 26, 30, 170, 198
DpnII GATC 4 cut(s) 24, 28, 168, 196
Eam1104I CTCTTC 1 cut(s) 559
EarI CTCTTC 1 cut(s) 559
Eco130I CCWWGG 1 cut(s) 531
EcoT14I CCWWGG 1 cut(s) 531
EgeI GGCGCC 1 cut(s) 88
EheI GGCGCC 1 cut(s) 88
ErhI CCWWGG 1 cut(s) 531
FaeI CATG 7 cut(s) 16, 35, 56, 184, 203, 223, 559
FaqI GGGAC 1 cut(s) 625
FatI CATG 7 cut(s) 12, 31, 52, 180, 199, 219, 555
Fnu4HI GCNGC 5 cut(s) 85, 94, 309, 312, 315
FokI GGATG 5 cut(s) 53, 220, 323, 458, 590
Fsp4HI GCNGC 5 cut(s) 85, 94, 309, 312, 315
FspBI CTAG 2 cut(s) 425, 455
GlaI GCGC 3 cut(s) 88, 97, 285
GluI GCNGC 5 cut(s) 85, 94, 309, 312, 315
GsuI CTGGAG 2 cut(s) 308, 540
HaeII RGCGCY 3 cut(s) 90, 99, 287
HapII CCGG 8 cut(s) 72, 90, 126, 144, 162, 239, 278, 296
HgaI GACGC 1 cut(s) 139
HhaI GCGC 3 cut(s) 89, 98, 286
Hin1I GRCGYC 2 cut(s) 87, 150
Hin1II CATG 7 cut(s) 16, 35, 56, 184, 203, 223, 559
Hin6I GCGC 3 cut(s) 87, 96, 284
HinP1I GCGC 3 cut(s) 87, 96, 284
HinfI GANTC 2 cut(s) 381, 463
HpaII CCGG 8 cut(s) 72, 90, 126, 144, 162, 239, 278, 296
HphI GGTGA 2 cut(s) 29, 197
Hpy188I TCNGA 5 cut(s) 155, 173, 376, 468, 608
Hpy99I CGWCG 2 cut(s) 155, 392
HpyCH4IV ACGT 1 cut(s) 390
HpyF3I CTNAG 1 cut(s) 375
HpySE526I ACGT 1 cut(s) 390
Hsp92I GRCGYC 2 cut(s) 87, 150
Hsp92II CATG 7 cut(s) 16, 35, 56, 184, 203, 223, 559
HspAI GCGC 3 cut(s) 87, 96, 284
KasI GGCGCC 1 cut(s) 86
KroI GCCGGC 3 cut(s) 89, 161, 295
KroNI GCCGGC 3 cut(s) 91, 163, 297
Kzo9I GATC 4 cut(s) 24, 28, 168, 196
LweI GCATC 1 cut(s) 225
MaeI CTAG 2 cut(s) 425, 455
MaeII ACGT 1 cut(s) 390
MalI GATC 4 cut(s) 26, 30, 170, 198
MboI GATC 4 cut(s) 24, 28, 168, 196
MboII GAAGA 1 cut(s) 576
MflI RGATCY 1 cut(s) 168
MluCI AATT 3 cut(s) 348, 418, 491
Mly113I GGCGCC 1 cut(s) 87
MlyI GAGTC 1 cut(s) 472
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 8 cut(s) 13, 149, 181, 283, 370, 405, 560, 602
MreI CGCCGGCG 1 cut(s) 89
MroNI GCCGGC 3 cut(s) 89, 161, 295
MseI TTAA 3 cut(s) 9, 177, 347
MspI CCGG 8 cut(s) 72, 90, 126, 144, 162, 239, 278, 296
MspR9I CCNGG 1 cut(s) 127
NaeI GCCGGC 3 cut(s) 91, 163, 297
NarI GGCGCC 1 cut(s) 87
NciI CCSGG 1 cut(s) 127
NdeII GATC 4 cut(s) 24, 28, 168, 196
NgoMIV GCCGGC 3 cut(s) 89, 161, 295
NlaIII CATG 7 cut(s) 16, 35, 56, 184, 203, 223, 559
NlaIV GGNNCC 7 cut(s) 70, 88, 124, 142, 160, 276, 294
PdiI GCCGGC 3 cut(s) 91, 163, 297
PfeI GAWTC 1 cut(s) 381
PkrI GCNGC 5 cut(s) 86, 95, 310, 313, 316
PleI GAGTC 1 cut(s) 471
PluTI GGCGCC 1 cut(s) 90
PpsI GAGTC 1 cut(s) 471
PspN4I GGNNCC 7 cut(s) 70, 88, 124, 142, 160, 276, 294
PsuI RGATCY 1 cut(s) 168
RsaI GTAC 2 cut(s) 76, 243
RsaNI GTAC 2 cut(s) 75, 242
SaqAI TTAA 3 cut(s) 9, 177, 347
SatI GCNGC 5 cut(s) 85, 94, 309, 312, 315
Sau3AI GATC 4 cut(s) 24, 28, 168, 196
SchI GAGTC 1 cut(s) 472
ScrFI CCNGG 1 cut(s) 127
SetI ASST 5 cut(s) 160, 294, 393, 441, 475
SfaNI GCATC 1 cut(s) 225
SfoI GGCGCC 1 cut(s) 88
SgrAI CRCCGGYG 1 cut(s) 89
Sse9I AATT 3 cut(s) 348, 418, 491
SspDI GGCGCC 1 cut(s) 86
SspMI CTAG 2 cut(s) 425, 455
StyD4I CCNGG 1 cut(s) 125
StyI CCWWGG 1 cut(s) 531
TaiI ACGT 1 cut(s) 393
TaqI TCGA 3 cut(s) 27, 540, 583
TasI AATT 3 cut(s) 348, 418, 491
TauI GCSGC 5 cut(s) 87, 96, 311, 314, 317
TfiI GAWTC 1 cut(s) 381
Tru1I TTAA 3 cut(s) 9, 177, 347
Tru9I TTAA 3 cut(s) 9, 177, 347
TscAI CASTG 3 cut(s) 264, 324, 330
TspDTI ATGAA 3 cut(s) 69, 236, 579
TspRI CASTG 3 cut(s) 264, 324, 330
XapI RAATTY 1 cut(s) 491
XspI CTAG 2 cut(s) 425, 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.