Rh2CG545600

Pentatricopeptide repeat-containing protein At1g03560

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0003325
Physical Location & Seq
Reverse (-)
72323465 .. 72324526
1062 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG545600.1

Sequence Viewer

Length: 1062 bp
ATGATCTCCGAGGAAAGCCCGAGACAGCTCTCCGGTTTTTCTCCTGGGCCGGTAAGCAAGCAAAAGAAGTATCATCATAAGCTTGAATGCTATGTGTCTTTGATTGAGCTTCTGTGTTTGTGTGGTGATTTGGGTAGGATTACATGTGTTGTTGTTGAGCTTAGAAAGATGAACTTTTTGATGAATTCGAATGCGGCGAATTGTTTGATTAAGAGTTTTGGCTGTCTTGGAATGGTTGAGGAGTTGTTGTGGGTTTGGCGTGGGATGAAGGAGAATGGGATTGAGCCTAGTTTGTATACTTACAACTTTTTGGTGAATGGATTGGTGAACTCGATGTTTATTGAATCCGCGGAGCGGGTTTTCGAGGTTATGGAAGGTGGGAAGATTGTGCCTGACATTGTGACTTATAATACAATGATTAAAGGGTATTATTGTAAGGCGGGGAAAACCCAGAAGGCAATGGAGAAGTTTATGGATATGGAGGGGAGGAATGTGGAGGCTGATAAGATTACTTATATGAATTTGATGCAGGGGTGTTATTCGGAAGGAGATTTCGATTCATGTTTGAGTCTGTACCAGGAAATGAGAGAGAAGGGGATTGAAATTCCGTCTCACGCGTATAGTTTAGTCATGAATGGGCTATGTAAAGGTGGGAAATGTATGGTTGGGTATGCTGTTTTTGAAGATATGATTGAGAAGGGTTGTAAAGCAAATGTGGCAAACTATACAGCTTTGATCGATTCATATGCAAAATGTGGGTGCATTGAAGAGGCAATGAAGCTTTTCGAGAGGATGAAGAGCGATGGGCTTGAACCAGATGGGGTTACTTATGGGGTTATTGTCAATGGATTATGTAAGAGTGGGAGATTGGATAAGGCCATGGAGTATTTTCAGTTTTGTCAAAACAGCAGAATGGCAGACAATGCTATGTTGTATTCTAGTTTAATCGATGGTCTTGGAAAGCCTGGAAGGGTTGATGAAGCTGAAAGGGTATTCGAAAAGATGATCGTAAATGGTTGTCCACCGGATTCATACTGCTATAATGCCCTTATCGATGCCTAA

Protein Analysis

353

Amino Acids

39.56

Weight (kDa)

5.21

Isoelectric Point (pI)

34.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 63 - 110 3.3e-08 PPR repeat family
PPR_3 PF13812 87 - 143 2.9e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 129 - 161 1.1e-08 PPR repeat
PPR_2 PF13041 131 - 179 2.9e-12 PPR repeat family
PPR_2 PF13041 168 - 216 9.2e-11 PPR repeat family
PPR PF01535 170 - 200 3.1e-06 PPR repeat
PPR_long PF17177 175 - 317 1.5e-13 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 205 - 247 2.5e-06 PPR repeat family
TPR_24 PF23276 224 - 333 5.9e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 226 - 282 1.4e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 233 - 266 2.4e-08 PPR repeat
PPR_2 PF13041 238 - 286 6.8e-16 PPR repeat family
PPR PF01535 242 - 270 1.4e-09 PPR repeat
PPR_1 PF12854 268 - 297 9.1e-10 PPR repeat
PPR_1 PF12854 308 - 335 1.2e-07 PPR repeat
PPR_2 PF13041 308 - 353 1.6e-11 PPR repeat family
PPR PF01535 312 - 340 2.5e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 408
AccBSI CCGCTC 1 cut(s) 355
AccI GTMKAC 1 cut(s) 296
AccII CGCG 2 cut(s) 350, 617
AciI CCGC 5 cut(s) 194, 348, 350, 355, 440
AcsI RAATTY 3 cut(s) 184, 520, 603
AfaI GTAC 1 cut(s) 575
AfiI CCNNNNNNNGG 1 cut(s) 354
AflIII ACRYGT 2 cut(s) 143, 615
AgsI TTSAA 6 cut(s) 86, 344, 602, 683, 767, 812
AjnI CCWGG 3 cut(s) 43, 576, 964
AluBI AGCT 7 cut(s) 28, 82, 109, 160, 731, 781, 983
AluI AGCT 7 cut(s) 28, 82, 109, 160, 731, 781, 983
Alw26I GTCTC 2 cut(s) 16, 615
Ama87I CYCGRG 1 cut(s) 19
AoxI GGCC 2 cut(s) 47, 876
ApoI RAATTY 3 cut(s) 184, 520, 603
Asp700I GAANNNNTTC 1 cut(s) 782
AspS9I GGNCC 1 cut(s) 47
AsuHPI GGTGA 3 cut(s) 137, 325, 337
AsuII TTCGAA 2 cut(s) 188, 996
AvaI CYCGRG 1 cut(s) 19
BccI CCATC 3 cut(s) 797, 812, 944
BcgI CGANNNNNNTGC 2 cut(s) 728, 762
BciT130I CCWGG 3 cut(s) 45, 578, 966
BcoDI GTCTC 2 cut(s) 16, 615
BfaI CTAG 2 cut(s) 288, 939
BisI GCNGC 1 cut(s) 195
BlsI GCNGC 1 cut(s) 196
Bme1390I CCNGG 3 cut(s) 45, 578, 966
BmeT110I CYCGRG 1 cut(s) 19
BmgT120I GGNCC 1 cut(s) 47
BmrFI CCNGG 3 cut(s) 45, 578, 966
BmsI GCATC 2 cut(s) 516, 1045
Bpu14I TTCGAA 2 cut(s) 188, 996
Bsa29I ATCGAT 3 cut(s) 738, 948, 1053
BsaJI CCNNGG 4 cut(s) 9, 44, 348, 879
BsaWI WCCGGW 2 cut(s) 32, 1024
BsaXI ACNNNNNCTCC 2 cut(s) 233, 263
Bsc4I CCNNNNNNNGG 1 cut(s) 354
Bse118I RCCGGY 1 cut(s) 49
Bse3DI GCAATG 2 cut(s) 465, 780
BseBI CCWGG 3 cut(s) 45, 578, 966
BseCI ATCGAT 3 cut(s) 738, 948, 1053
BseDI CCNNGG 4 cut(s) 9, 44, 348, 879
BseGI GGATG 2 cut(s) 270, 798
BseLI CCNNNNNNNGG 1 cut(s) 354
BseMI GCAATG 2 cut(s) 465, 780
BseRI GAGGAG 1 cut(s) 254
Bsh1236I CGCG 2 cut(s) 350, 617
BshFI GGCC 2 cut(s) 49, 878
BshVI ATCGAT 3 cut(s) 738, 948, 1053
BsiHKCI CYCGRG 1 cut(s) 19
BsiSI CCGG 3 cut(s) 33, 50, 1025
BslI CCNNNNNNNGG 1 cut(s) 354
BsmAI GTCTC 2 cut(s) 16, 615
BsmBI CGTCTC 1 cut(s) 615
BsmI GAATGC 2 cut(s) 92, 196
BsnI GGCC 2 cut(s) 49, 878
BsoBI CYCGRG 1 cut(s) 19
Bsp119I TTCGAA 2 cut(s) 188, 996
Bsp143I GATC 3 cut(s) 3, 735, 1005
Bsp19I CCATGG 1 cut(s) 879
BspACI CCGC 5 cut(s) 194, 348, 350, 355, 440
BspANI GGCC 2 cut(s) 49, 878
BspDI ATCGAT 3 cut(s) 738, 948, 1053
BspFNI CGCG 2 cut(s) 350, 617
BspHI TCATGA 1 cut(s) 630
BspQI GCTCTTC 1 cut(s) 791
BspT104I TTCGAA 2 cut(s) 188, 996
BsrBI CCGCTC 1 cut(s) 355
BsrDI GCAATG 2 cut(s) 465, 780
BsrFI RCCGGY 1 cut(s) 49
BssAI RCCGGY 1 cut(s) 49
BssECI CCNNGG 4 cut(s) 9, 44, 348, 879
BssMI GATC 3 cut(s) 3, 735, 1005
BssNAI GTATAC 1 cut(s) 297
BssT1I CCWWGG 1 cut(s) 879
Bst1107I GTATAC 1 cut(s) 297
Bst2UI CCWGG 3 cut(s) 45, 578, 966
Bst6I CTCTTC 2 cut(s) 762, 791
BstAPI GCANNNNNTGC 1 cut(s) 923
BstBI TTCGAA 2 cut(s) 188, 996
BstC8I GCNNGC 1 cut(s) 59
BstDEI CTNAG 1 cut(s) 161
BstDSI CCRYGG 2 cut(s) 348, 879
BstF5I GGATG 2 cut(s) 270, 798
BstFNI CGCG 2 cut(s) 350, 617
BstKTI GATC 3 cut(s) 6, 738, 1008
BstMAI GTCTC 2 cut(s) 16, 615
BstMBI GATC 3 cut(s) 3, 735, 1005
BstMWI GCNNNNNNNGC 2 cut(s) 716, 923
BstNI CCWGG 3 cut(s) 45, 578, 966
BstNSI RCATGY 1 cut(s) 147
BstSCI CCNGG 3 cut(s) 43, 576, 964
BstUI CGCG 2 cut(s) 350, 617
BstZ17I GTATAC 1 cut(s) 297
Bsu15I ATCGAT 3 cut(s) 738, 948, 1053
BsuRI GGCC 2 cut(s) 49, 878
BsuTUI ATCGAT 3 cut(s) 738, 948, 1053
BtgI CCRYGG 2 cut(s) 348, 879
BtgZI GCGATG 1 cut(s) 816
BtsCI GGATG 2 cut(s) 270, 798
Cac8I GCNNGC 1 cut(s) 59
CciI TCATGA 1 cut(s) 630
Cfr10I RCCGGY 1 cut(s) 49
Cfr13I GGNCC 1 cut(s) 47
Cfr42I CCGCGG 1 cut(s) 351
ClaI ATCGAT 3 cut(s) 738, 948, 1053
Csp6I GTAC 1 cut(s) 574
CviAII CATG 4 cut(s) 144, 561, 631, 880
CviQI GTAC 1 cut(s) 574
DdeI CTNAG 1 cut(s) 161
DpnI GATC 3 cut(s) 5, 737, 1007
DpnII GATC 3 cut(s) 3, 735, 1005
Eam1104I CTCTTC 2 cut(s) 762, 791
EarI CTCTTC 2 cut(s) 762, 791
Eco130I CCWWGG 1 cut(s) 879
Eco88I CYCGRG 1 cut(s) 19
EcoRI GAATTC 1 cut(s) 184
EcoRII CCWGG 3 cut(s) 43, 576, 964
EcoT14I CCWWGG 1 cut(s) 879
ErhI CCWWGG 1 cut(s) 879
Esp3I CGTCTC 1 cut(s) 615
FaeI CATG 4 cut(s) 147, 564, 634, 883
FalI AAGNNNNNCTT 2 cut(s) 158, 190
FatI CATG 4 cut(s) 143, 560, 630, 879
FauI CCCGC 2 cut(s) 348, 433
FauNDI CATATG 1 cut(s) 745
FblI GTMKAC 1 cut(s) 296
Fnu4HI GCNGC 1 cut(s) 195
FokI GGATG 2 cut(s) 277, 805
Fsp4HI GCNGC 1 cut(s) 195
FspBI CTAG 2 cut(s) 288, 939
GluI GCNGC 1 cut(s) 195
HaeIII GGCC 2 cut(s) 49, 878
HapII CCGG 3 cut(s) 33, 50, 1025
Hin1II CATG 4 cut(s) 147, 564, 634, 883
HindIII AAGCTT 2 cut(s) 80, 779
HinfI GANTC 5 cut(s) 344, 557, 568, 740, 1028
HpaII CCGG 3 cut(s) 33, 50, 1025
HphI GGTGA 3 cut(s) 137, 325, 337
Hpy166II GTNNAC 3 cut(s) 297, 328, 1022
Hpy188I TCNGA 2 cut(s) 10, 544
Hpy188III TCNNGA 2 cut(s) 631, 787
Hpy8I GTNNAC 3 cut(s) 297, 328, 1022
HpyAV CCTTC 7 cut(s) 262, 368, 448, 539, 586, 691, 963
HpyCH4V TGCA 3 cut(s) 529, 749, 762
HpyF10VI GCNNNNNNNGC 2 cut(s) 716, 923
HpyF3I CTNAG 1 cut(s) 161
Hsp92II CATG 4 cut(s) 147, 564, 634, 883
KspI CCGCGG 1 cut(s) 351
Kzo9I GATC 3 cut(s) 3, 735, 1005
LguI GCTCTTC 1 cut(s) 791
LmnI GCTCC 1 cut(s) 352
LweI GCATC 2 cut(s) 516, 1045
MaeI CTAG 2 cut(s) 288, 939
MaeIII GTNAC 2 cut(s) 400, 823
MalI GATC 3 cut(s) 5, 737, 1007
MbiI CCGCTC 1 cut(s) 355
MboI GATC 3 cut(s) 3, 735, 1005
MboII GAAGA 4 cut(s) 394, 695, 779, 808
MluCI AATT 4 cut(s) 184, 199, 520, 603
MluI ACGCGT 1 cut(s) 615
MlyI GAGTC 1 cut(s) 577
MnlI CCTC 8 cut(s) 4, 232, 358, 475, 480, 490, 763, 783
MroXI GAANNNNTTC 1 cut(s) 782
MseI TTAA 3 cut(s) 210, 420, 944
MspA1I CMGCKG 1 cut(s) 350
MspI CCGG 3 cut(s) 33, 50, 1025
MspR9I CCNGG 3 cut(s) 45, 578, 966
Mva1269I GAATGC 2 cut(s) 92, 196
MvaI CCWGG 3 cut(s) 45, 578, 966
MvnI CGCG 2 cut(s) 350, 617
MwoI GCNNNNNNNGC 2 cut(s) 716, 923
NcoI CCATGG 1 cut(s) 879
NdeI CATATG 1 cut(s) 745
NdeII GATC 3 cut(s) 3, 735, 1005
NlaIII CATG 4 cut(s) 147, 564, 634, 883
NmuCI GTSAC 1 cut(s) 400
NspI RCATGY 1 cut(s) 147
NspV TTCGAA 2 cut(s) 188, 996
PagI TCATGA 1 cut(s) 630
PciI ACATGT 1 cut(s) 143
PciSI GCTCTTC 1 cut(s) 791
PcsI WCGNNNNNNNCGW 1 cut(s) 194
PctI GAATGC 2 cut(s) 92, 196
PdmI GAANNNNTTC 1 cut(s) 782
PfeI GAWTC 4 cut(s) 344, 557, 740, 1028
PkrI GCNGC 1 cut(s) 196
PleI GAGTC 1 cut(s) 576
PpsI GAGTC 1 cut(s) 576
PscI ACATGT 1 cut(s) 143
PsiI TTATAA 1 cut(s) 408
Psp6I CCWGG 3 cut(s) 43, 576, 964
PspGI CCWGG 3 cut(s) 43, 576, 964
PspPI GGNCC 1 cut(s) 47
RsaI GTAC 1 cut(s) 575
RsaNI GTAC 1 cut(s) 574
SacII CCGCGG 1 cut(s) 351
SapI GCTCTTC 1 cut(s) 791
SaqAI TTAA 3 cut(s) 210, 420, 944
SatI GCNGC 1 cut(s) 195
Sau3AI GATC 3 cut(s) 3, 735, 1005
Sau96I GGNCC 1 cut(s) 47
SchI GAGTC 1 cut(s) 577
ScrFI CCNGG 3 cut(s) 45, 578, 966
SfaNI GCATC 2 cut(s) 516, 1045
Sfr303I CCGCGG 1 cut(s) 351
SfuI TTCGAA 2 cut(s) 188, 996
SgrBI CCGCGG 1 cut(s) 351
Sse9I AATT 4 cut(s) 184, 199, 520, 603
SsiI CCGC 5 cut(s) 194, 348, 350, 355, 440
SspMI CTAG 2 cut(s) 288, 939
StyD4I CCNGG 3 cut(s) 43, 576, 964
StyI CCWWGG 1 cut(s) 879
TaqI TCGA 9 cut(s) 188, 332, 363, 555, 738, 786, 948, 996, 1053
TasI AATT 4 cut(s) 184, 199, 520, 603
TauI GCSGC 1 cut(s) 197
TfiI GAWTC 4 cut(s) 344, 557, 740, 1028
Tru1I TTAA 3 cut(s) 210, 420, 944
Tru9I TTAA 3 cut(s) 210, 420, 944
TseFI GTSAC 1 cut(s) 400
Tsp45I GTSAC 1 cut(s) 400
TspGWI ACGGA 1 cut(s) 597
XapI RAATTY 3 cut(s) 184, 520, 603
XceI RCATGY 1 cut(s) 147
XmiI GTMKAC 1 cut(s) 296
XmnI GAANNNNTTC 1 cut(s) 782
XspI CTAG 2 cut(s) 288, 939
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.