pycom03g13590

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0006905
Physical Location & Seq
Forward (+)
14720884 .. 14724354
3471 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g13590.1

Sequence Viewer

Length: 2121 bp
ATGGGTCTTGGCGGAGTTATTCCTCCGCATCTAGGCAACCTCTCATTTCTCGTTGAGTTGGGCCTTAATAATAATAGCTTTCATGGTCCCCTACCGCAAGAACTATCTCGTTTGCGCCGGTTGAAGGCGATTAACTTTGGAAACAACAGCTTTATGGGAACCATTCCTTCGTGGTTTGGGTCCTTTCCTAAACTTCAAACCATCAAATTGTACGGTAATGGCTTCTCTGGTTTCATACCCGCTGCTATCTTCAACTTATCTGGACTCGAAATAATTAATCTGAGCAGGAACCAACTATCAGGTAGCATACCCAGAGAAATCGGCAACTTAACAATGGTGAAGGGCATATACCTTGACGACAACAAGTTCGAAGGTAGCATACCCAGAGAAATAGGCAACTTAACAATGGTGAAGGTCATAAACCTTGACGACAACAAGTTCGAAGAACTTCCAAACGAGATGAGCAGTTTAGGCCAGCTGGAGGAGTTGTATGTGCAGTACAATGCCCTAAAAGGCTCTGCTCTTGTGCCTGTCCTCAACATATCTTCTTTGACTACTTTGGCTCTATATGGAAACAACATGAGTGGCAGTCTTCCGGACAATATATGTGAGCATCTTCCGAGTATTCGAATATTTAATTTGGGTCGAAACCAGTTTGACGGTCTTATTCCATCCAAACTATGGCAATGCAAAGAGCTTCGTGAATTGGTATTAGGTGATAACAATTTCAGTGGAAGCATACCCAAAAGTCTTGGCAATTTGACCTACTTAACCAAGATTTATCTTGATGAGAATAATTTAACAGGTACAATACCGGATGAGATTGGTGATCTTCCGCAATTAGAGATTTTGGGACTGGGGATTAATAATCTCAATGGCGTCATCCCATCCAAACTCTTCAATAACTCCATGATAAGAGGAATAGGGCTTTATTTTAATCAGCTCTCAGGTAGTCTCCCAGCAAACATAGGTCTTAACGTTCCAAACCTAGAAGTCCTTGTATTAGCCAGAAATAACTTCGTGGGCGGACTACTCCCTAACCTCTCTAATGCTTCCAAGCTTGAGGTGCTAGACATGAACACAAACTCATTTACCGGGGTTCTTCCTAGCACGCTCTGTTCCTTGAAAAACCTCCAGGTTCTTAACTTGCACTCGAATAATTTGACAATTGATGCTTCTACTCCACAAGCAGCAAGCACTCTCACTTGCTTGTTTAATCTTAGAAATTTGACAAGATTATCCTTAGGAAACAATCCACTAAACACTATGATTCCAGCTTCTCACAGGAATCTCTCTACATCACTCCTATATGTTGTTTTAAGCAATTCCAACATCAGGGGTAACATTCCAGTTGATATTGGCAACTTTAGCAACTTGATATCATTAAGCCTGGGAAACAATCAGTTGAGGGGAGCAATTCCAACTTCAATACAAAGGCTACAAAATCTCCAAGCTTTGTATTTGAATGATAACGAACTGCAAGGAAATGTCCCGTATGAACTCTGTCAACTAAACAACCTAGTCGAGTTACTTTTGGCTGGTAATCGTCTCTCTGGTTCTATTCCTTCCTGCTTGGGTACTTTGGCAGTAGCTCTAAGAGATTTATCGTTAGACTCCAATTTGCTAACTTCAAAAATACCATCTTCCTTGTGGGAACTCAAGTATATATTGTTCCTAAACTTGTCATCCAATTCTCTAGTTGGACCACTCTCAGAAGACATCGGAAAGTTAAAAGATGTGGTGGATATAGATTTATCAAATAATCACTTCACTGGAAACATTCCCAGTAGCATTGGTGGTCTTCAAAATATGATTAATTTGTCCTTGGCAAACAATTATTTAGAAGGCCCTATTCCCACTTCATTTAAAACCTTGGTAAGCCTAGAATTCTTGGATTTGTCCCAAAACAATCTATCTGGAGAGATCCCAAAGTCATTGGAAGCACTCTTGTATCTCAAGCATCTAAATTTGTCTTTCAACAAACTCCACGGTGAAATTCCAAACGGAGGGCCTTTTGGAAACTTCTCTGCTGATTCATTTGTATCAAACGCTGCACTCTGCGGTGCTTCCCGACTCCATGTTCCACTGTGCAAAAATAGAACAAAAGTTAAGCCAAATTAG

Protein Analysis

707

Amino Acids

76.64

Weight (kDa)

6.18

Isoelectric Point (pI)

27.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 92 - 189 1.4e-07 Leucine-rich repeat region
LRR_8 PF13855 183 - 243 2.2e-06 Leucine rich repeat
LRR_14 PF23598 218 - 419 1.2e-07 Leucine-rich repeat region
LRR_8 PF13855 232 - 291 1.8e-07 Leucine rich repeat
LRR_8 PF13855 458 - 517 1.9e-07 Leucine rich repeat
LRR_14 PF23598 548 - 662 1.7e-08 Leucine-rich repeat region
LRR_8 PF13855 583 - 638 1.6e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 681
AccIII TCCGGA 1 cut(s) 595
AciI CCGC 7 cut(s) 12, 26, 95, 240, 836, 1026, 2061
AclI AACGTT 1 cut(s) 978
AclWI GGATC 1 cut(s) 1918
AcsI RAATTY 4 cut(s) 1225, 1886, 1966, 1995
AcyI GRCGYC 1 cut(s) 879
AfaI GTAC 4 cut(s) 212, 500, 808, 1579
AfiI CCNNNNNNNGG 6 cut(s) 32, 124, 481, 681, 1335, 2006
AjnI CCWGG 2 cut(s) 1134, 1389
AluBI AGCT 9 cut(s) 78, 150, 478, 697, 943, 1060, 1277, 1454, 1592
AluI AGCT 9 cut(s) 78, 150, 478, 697, 943, 1060, 1277, 1454, 1592
Alw26I GTCTC 2 cut(s) 959, 1553
AlwI GGATC 1 cut(s) 1918
Aor13HI TCCGGA 1 cut(s) 595
AoxI GGCC 4 cut(s) 61, 472, 1846, 2009
ApeKI GCWGC 3 cut(s) 242, 1190, 2051
ApoI RAATTY 4 cut(s) 1225, 1886, 1966, 1995
ArsI GACNNNNNNTTYG 4 cut(s) 350, 382, 422, 454
AseI ATTAAT 3 cut(s) 276, 864, 1815
Asp700I GAANNNNTTC 1 cut(s) 447
AspLEI GCGC 1 cut(s) 117
AspS9I GGNCC 6 cut(s) 61, 86, 180, 1703, 1847, 2009
AsuC2I CCSGG 1 cut(s) 1096
AsuHPI GGTGA 5 cut(s) 349, 421, 728, 839, 2003
AsuII TTCGAA 3 cut(s) 369, 441, 628
AvaII GGWCC 3 cut(s) 86, 180, 1703
AxyI CCTNAGG 1 cut(s) 1243
BaeI ACNNNNGTAYC 2 cut(s) 1934, 1967
BarI GAAGNNNNNNTAC 4 cut(s) 332, 363, 364, 395
BbsI GAAGAC 3 cut(s) 584, 1722, 1793
BbvI GCAGC 3 cut(s) 229, 1202, 2038
BccI CCATC 4 cut(s) 209, 679, 895, 1648
BciT130I CCWGG 2 cut(s) 1136, 1391
BcnI CCSGG 1 cut(s) 1096
BcoDI GTCTC 2 cut(s) 959, 1553
BfaI CTAG 7 cut(s) 32, 989, 1070, 1107, 1520, 1697, 1883
BisI GCNGC 3 cut(s) 243, 1191, 2052
BlsI GCNGC 3 cut(s) 244, 1192, 2053
Bme1390I CCNGG 3 cut(s) 1096, 1136, 1391
Bme18I GGWCC 3 cut(s) 86, 180, 1703
BmgT120I GGNCC 6 cut(s) 61, 86, 180, 1703, 1847, 2009
BmiI GGNNCC 4 cut(s) 88, 160, 181, 290
BmrFI CCNGG 3 cut(s) 1096, 1136, 1391
BmrI ACTGGG 2 cut(s) 866, 1779
BmsI GCATC 4 cut(s) 37, 622, 1162, 1969
BmuI ACTGGG 2 cut(s) 866, 1779
BpiI GAAGAC 3 cut(s) 584, 1722, 1793
BpmI CTGGAG 3 cut(s) 500, 1118, 1938
Bpu14I TTCGAA 3 cut(s) 369, 441, 628
BpuEI CTTGAG 3 cut(s) 1082, 1643, 1940
BpuMI CCSGG 1 cut(s) 1096
BsaHI GRCGYC 1 cut(s) 879
BsaJI CCNNGG 5 cut(s) 1095, 1390, 1824, 1872, 1987
BsaWI WCCGGW 2 cut(s) 595, 814
BsaXI ACNNNNNCTCC 4 cut(s) 473, 503, 1431, 1461
Bsc4I CCNNNNNNNGG 6 cut(s) 32, 124, 481, 681, 1335, 2006
Bse118I RCCGGY 1 cut(s) 117
Bse1I ACTGG 5 cut(s) 652, 861, 1349, 1777, 1785
Bse21I CCTNAGG 1 cut(s) 1243
Bse3DI GCAATG 1 cut(s) 692
BseAI TCCGGA 1 cut(s) 595
BseBI CCWGG 2 cut(s) 1136, 1391
BseDI CCNNGG 5 cut(s) 1095, 1390, 1824, 1872, 1987
BseGI GGATG 5 cut(s) 671, 823, 882, 887, 1685
BseLI CCNNNNNNNGG 6 cut(s) 32, 124, 481, 681, 1335, 2006
BseMI GCAATG 1 cut(s) 692
BseMII CTCAG 3 cut(s) 272, 960, 1725
BseNI ACTGG 5 cut(s) 652, 861, 1349, 1777, 1785
BseRI GAGGAG 1 cut(s) 497
BseXI GCAGC 3 cut(s) 229, 1202, 2038
BseYI CCCAGC 1 cut(s) 958
BsgI GTGCAG 2 cut(s) 515, 2037
BshFI GGCC 4 cut(s) 63, 474, 1848, 2011
BsiSI CCGG 4 cut(s) 118, 596, 815, 1095
BslFI GGGAC 4 cut(s) 72, 867, 1475, 1885
BslI CCNNNNNNNGG 6 cut(s) 32, 124, 481, 681, 1335, 2006
BsmAI GTCTC 2 cut(s) 959, 1553
BsmBI CGTCTC 1 cut(s) 1553
BsmFI GGGAC 4 cut(s) 72, 867, 1475, 1885
BsnI GGCC 4 cut(s) 63, 474, 1848, 2011
Bsp119I TTCGAA 3 cut(s) 369, 441, 628
Bsp13I TCCGGA 1 cut(s) 595
Bsp143I GATC 2 cut(s) 829, 1923
BspACI CCGC 7 cut(s) 12, 26, 95, 240, 836, 1026, 2061
BspANI GGCC 4 cut(s) 63, 474, 1848, 2011
BspCNI CTCAG 3 cut(s) 273, 959, 1724
BspEI TCCGGA 1 cut(s) 595
BspLI GGNNCC 4 cut(s) 88, 160, 181, 290
BspPI GGATC 1 cut(s) 1918
BspT104I TTCGAA 3 cut(s) 369, 441, 628
BsrDI GCAATG 1 cut(s) 692
BsrFI RCCGGY 1 cut(s) 117
BsrI ACTGG 5 cut(s) 652, 861, 1349, 1777, 1785
BssAI RCCGGY 1 cut(s) 117
BssECI CCNNGG 5 cut(s) 1095, 1390, 1824, 1872, 1987
BssMI GATC 2 cut(s) 829, 1923
BssNI GRCGYC 1 cut(s) 879
BssT1I CCWWGG 2 cut(s) 1824, 1872
Bst2UI CCWGG 2 cut(s) 1136, 1391
Bst4CI ACNGT 4 cut(s) 215, 662, 1991, 2088
Bst6I CTCTTC 1 cut(s) 902
BstACI GRCGYC 1 cut(s) 879
BstBI TTCGAA 3 cut(s) 369, 441, 628
BstC8I GCNNGC 3 cut(s) 476, 1112, 1195
BstDEI CTNAG 6 cut(s) 281, 946, 1220, 1243, 1595, 1711
BstDSI CCRYGG 1 cut(s) 1987
BstF5I GGATG 5 cut(s) 671, 823, 882, 887, 1685
BstHHI GCGC 1 cut(s) 117
BstKTI GATC 2 cut(s) 832, 1926
BstMAI GTCTC 2 cut(s) 959, 1553
BstMBI GATC 2 cut(s) 829, 1923
BstMWI GCNNNNNNNGC 3 cut(s) 471, 1066, 1368
BstNI CCWGG 2 cut(s) 1136, 1391
BstSCI CCNGG 3 cut(s) 1094, 1134, 1389
BstV1I GCAGC 3 cut(s) 229, 1202, 2038
BstV2I GAAGAC 3 cut(s) 584, 1722, 1793
BstX2I RGATCY 1 cut(s) 1923
BstYI RGATCY 1 cut(s) 1923
Bsu36I CCTNAGG 1 cut(s) 1243
BsuRI GGCC 4 cut(s) 63, 474, 1848, 2011
BtgI CCRYGG 1 cut(s) 1987
BtsCI GGATG 5 cut(s) 671, 823, 882, 887, 1685
BtsIMutI CAGTG 3 cut(s) 736, 1770, 2084
Cac8I GCNNGC 3 cut(s) 476, 1112, 1195
CfoI GCGC 1 cut(s) 117
Cfr10I RCCGGY 1 cut(s) 117
Cfr13I GGNCC 6 cut(s) 61, 86, 180, 1703, 1847, 2009
CseI GACGC 1 cut(s) 868
Csp6I GTAC 4 cut(s) 211, 499, 807, 1578
CspCI CAANNNNNGTGG 4 cut(s) 565, 600, 712, 747
CviAII CATG 5 cut(s) 83, 580, 910, 1075, 2078
CviQI GTAC 4 cut(s) 211, 499, 807, 1578
DdeI CTNAG 6 cut(s) 281, 946, 1220, 1243, 1595, 1711
DpnI GATC 2 cut(s) 831, 1925
DpnII GATC 2 cut(s) 829, 1923
DraI TTTAAA 1 cut(s) 1867
Eam1104I CTCTTC 1 cut(s) 902
EarI CTCTTC 1 cut(s) 902
EciI GGCGGA 2 cut(s) 27, 1041
Eco130I CCWWGG 2 cut(s) 1824, 1872
Eco32I GATATC 1 cut(s) 1380
Eco47I GGWCC 3 cut(s) 86, 180, 1703
Eco81I CCTNAGG 1 cut(s) 1243
EcoO109I RGGNCCY 3 cut(s) 180, 1847, 2009
EcoRI GAATTC 1 cut(s) 1886
EcoRII CCWGG 2 cut(s) 1134, 1389
EcoRV GATATC 1 cut(s) 1380
EcoT14I CCWWGG 2 cut(s) 1824, 1872
ErhI CCWWGG 2 cut(s) 1824, 1872
Esp3I CGTCTC 1 cut(s) 1553
FaeI CATG 5 cut(s) 86, 583, 913, 1078, 2081
FaqI GGGAC 4 cut(s) 72, 867, 1475, 1885
FatI CATG 5 cut(s) 82, 579, 909, 1074, 2077
FauI CCCGC 1 cut(s) 247
Fnu4HI GCNGC 3 cut(s) 243, 1191, 2052
FokI GGATG 5 cut(s) 658, 830, 869, 874, 1672
Fsp4HI GCNGC 3 cut(s) 243, 1191, 2052
FspBI CTAG 7 cut(s) 32, 989, 1070, 1107, 1520, 1697, 1883
GlaI GCGC 1 cut(s) 116
GluI GCNGC 3 cut(s) 243, 1191, 2052
GsaI CCCAGC 1 cut(s) 962
GsuI CTGGAG 3 cut(s) 500, 1118, 1938
HaeIII GGCC 4 cut(s) 63, 474, 1848, 2011
HapII CCGG 4 cut(s) 118, 596, 815, 1095
HgaI GACGC 1 cut(s) 868
HhaI GCGC 1 cut(s) 117
Hin1I GRCGYC 1 cut(s) 879
Hin1II CATG 5 cut(s) 86, 583, 913, 1078, 2081
Hin6I GCGC 1 cut(s) 115
HinP1I GCGC 1 cut(s) 115
HincII GTYRAC 1 cut(s) 1508
HindII GTYRAC 1 cut(s) 1508
HindIII AAGCTT 2 cut(s) 1058, 1452
HinfI GANTC 6 cut(s) 264, 1270, 1288, 1613, 2033, 2073
HpaII CCGG 4 cut(s) 118, 596, 815, 1095
HphI GGTGA 5 cut(s) 349, 421, 728, 839, 2003
Hpy166II GTNNAC 1 cut(s) 1508
Hpy188I TCNGA 4 cut(s) 282, 621, 1714, 1724
Hpy188III TCNNGA 6 cut(s) 261, 596, 701, 785, 1917, 2070
Hpy8I GTNNAC 1 cut(s) 1508
HpyAV CCTTC 7 cut(s) 118, 177, 334, 365, 406, 1575, 1838
HpyCH4III ACNGT 4 cut(s) 215, 662, 1991, 2088
HpyCH4IV ACGT 1 cut(s) 978
HpyCH4V TGCA 6 cut(s) 496, 690, 1150, 1480, 2054, 2091
HpyF10VI GCNNNNNNNGC 3 cut(s) 471, 1066, 1368
HpyF3I CTNAG 6 cut(s) 281, 946, 1220, 1243, 1595, 1711
HpySE526I ACGT 1 cut(s) 978
Hsp92I GRCGYC 1 cut(s) 879
Hsp92II CATG 5 cut(s) 86, 583, 913, 1078, 2081
HspAI GCGC 1 cut(s) 115
Kpn2I TCCGGA 1 cut(s) 595
Kzo9I GATC 2 cut(s) 829, 1923
LmnI GCTCC 1 cut(s) 1412
Lsp1109I GCAGC 3 cut(s) 229, 1202, 2038
LweI GCATC 4 cut(s) 37, 622, 1162, 1969
MaeI CTAG 7 cut(s) 32, 989, 1070, 1107, 1520, 1697, 1883
MaeII ACGT 1 cut(s) 978
MaeIII GTNAC 2 cut(s) 1340, 1527
MalI GATC 2 cut(s) 831, 1925
MboI GATC 2 cut(s) 829, 1923
MfeI CAATTG 1 cut(s) 1167
MflI RGATCY 1 cut(s) 1923
MlyI GAGTC 3 cut(s) 258, 1607, 2067
MmeI TCCRAC 3 cut(s) 1353, 1445, 1681
MroI TCCGGA 1 cut(s) 595
MroXI GAANNNNTTC 1 cut(s) 447
MspA1I CMGCKG 2 cut(s) 242, 478
MspI CCGG 4 cut(s) 118, 596, 815, 1095
MspR9I CCNGG 3 cut(s) 1096, 1136, 1391
MunI CAATTG 1 cut(s) 1167
MvaI CCWGG 2 cut(s) 1136, 1391
MwoI GCNNNNNNNGC 3 cut(s) 471, 1066, 1368
NciI CCSGG 1 cut(s) 1096
NdeII GATC 2 cut(s) 829, 1923
NlaIII CATG 5 cut(s) 86, 583, 913, 1078, 2081
NlaIV GGNNCC 4 cut(s) 88, 160, 181, 290
NspV TTCGAA 3 cut(s) 369, 441, 628
PdmI GAANNNNTTC 1 cut(s) 447
PfeI GAWTC 3 cut(s) 1270, 1288, 2033
PflMI CCANNNNNTGG 1 cut(s) 681
PkrI GCNGC 3 cut(s) 244, 1192, 2053
PleI GAGTC 3 cut(s) 258, 1607, 2067
PpsI GAGTC 3 cut(s) 258, 1607, 2067
PpuMI RGGWCCY 1 cut(s) 180
PshBI ATTAAT 3 cut(s) 276, 864, 1815
Psp1406I AACGTT 1 cut(s) 978
Psp5II RGGWCCY 1 cut(s) 180
Psp6I CCWGG 2 cut(s) 1134, 1389
PspFI CCCAGC 1 cut(s) 958
PspGI CCWGG 2 cut(s) 1134, 1389
PspN4I GGNNCC 4 cut(s) 88, 160, 181, 290
PspPI GGNCC 6 cut(s) 61, 86, 180, 1703, 1847, 2009
PspPPI RGGWCCY 1 cut(s) 180
PsrI GAACNNNNNNTAC 2 cut(s) 1655, 1687
PsuI RGATCY 1 cut(s) 1923
PvuII CAGCTG 1 cut(s) 478
RsaI GTAC 4 cut(s) 212, 500, 808, 1579
RsaNI GTAC 4 cut(s) 211, 499, 807, 1578
SatI GCNGC 3 cut(s) 243, 1191, 2052
Sau3AI GATC 2 cut(s) 829, 1923
Sau96I GGNCC 6 cut(s) 61, 86, 180, 1703, 1847, 2009
SchI GAGTC 3 cut(s) 258, 1607, 2067
ScrFI CCNGG 3 cut(s) 1096, 1136, 1391
SfaNI GCATC 4 cut(s) 37, 622, 1162, 1969
SfuI TTCGAA 3 cut(s) 369, 441, 628
SinI GGWCC 3 cut(s) 86, 180, 1703
SmlI CTYRAG 3 cut(s) 1061, 1658, 1955
SmoI CTYRAG 3 cut(s) 1061, 1658, 1955
SsiI CCGC 7 cut(s) 12, 26, 95, 240, 836, 1026, 2061
SspI AATATT 1 cut(s) 633
SspMI CTAG 7 cut(s) 32, 989, 1070, 1107, 1520, 1697, 1883
StyD4I CCNGG 3 cut(s) 1094, 1134, 1389
StyI CCWWGG 2 cut(s) 1824, 1872
TaaI ACNGT 4 cut(s) 215, 662, 1991, 2088
TaiI ACGT 1 cut(s) 981
TaqI TCGA 7 cut(s) 267, 369, 441, 628, 646, 1154, 1524
TatI WGTACW 1 cut(s) 498
TfiI GAWTC 3 cut(s) 1270, 1288, 2033
TscAI CASTG 3 cut(s) 736, 1777, 2091
TseI GCWGC 3 cut(s) 242, 1190, 2051
TspDTI ATGAA 6 cut(s) 71, 223, 1091, 1512, 1851, 2025
TspGWI ACGGA 1 cut(s) 2019
TspRI CASTG 3 cut(s) 736, 1777, 2091
Van91I CCANNNNNTGG 1 cut(s) 681
VpaK11BI GGWCC 3 cut(s) 86, 180, 1703
VspI ATTAAT 3 cut(s) 276, 864, 1815
XapI RAATTY 4 cut(s) 1225, 1886, 1966, 1995
XcmI CCANNNNNNNNNTGG 2 cut(s) 678, 1647
XmnI GAANNNNTTC 1 cut(s) 447
XspI CTAG 7 cut(s) 32, 989, 1070, 1107, 1520, 1697, 1883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.