pycom03g16390

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0006631
Physical Location & Seq
Reverse (-)
17554778 .. 17556619
1842 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g16390.2

Sequence Viewer

Length: 939 bp
ATGGGAGGCAGCGGAGTCATGAGGTCGGTGGCAAAGGTGGCCGGTATCGGCGGCGTGGTCCACGGCGGCATGCTCCGTGGTGCGCCGGGGGTTCCTTTGTCGACTGGTCATTCGGTTCGCAAGGCCTCAATTCCCGTGGCAGCGACCCTCACGGCTCAGAACTACGGCGGAGGTGAAGTGGCAGCGATCGAGAAGCCTGCGTCGGACGCGGTCGACGACTTCGTCGACTGGCAGGTGGTCAGCGCCGGAGAAGATGAACTGGTGATGTCGGGCGGCGAGCCGATGCCGAGGGTTGTCTTCGACGCTGCGCCGAGCTTCAAGGAGGCCAAAGAGGCCACCGCTGAATTGAAAGACGCCCTCGATAAGGTGTATCTTTCTTCGCCCAAATCTACTGATTTGGGGGAGCCGTCTGCTGCTGCTGGTGCGGCTGGGCTGCCTCTGATAAAAAACCCTGAACCTGAGGAGATGGGATCCCTACTTCCTACTAGGACTTCAGTGCCGAAACATGCTCTTCAAGCATTTGAAATGCTGAGTAGAAGTTCTGAAGCTCAGAATGTTGTTGCTTCAATTACAAGTGACCCAAATATCTGGAATGCTATGATGGAAAATTCTGCGGTCAAGCAATTTATGGCCAACAAGAACTGTAATCTTAACACGGAAGAGTATGCTGCAGATACTTATCCAGCTTCACCCAAGAAGCTTGAAGATGAATCAGAGAAATCTAACAGCTTGGAAGATATGCTCGGTGGTTTTGTGCAGAAAATCAAGCTTACTGTGGATAAGTGGGTCAATGATTTGTCGTCATTCATCCAGAACATTTTCGAACCCCCTGCAGCTGAGACTGAAGCAGACGGAGGGAATACAGGAGCGGTTGTAGCATCTGCGTTCATGGGACTGGCGGTTATGGTTGTTATGGTGGTCTTGACGAAGCGTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

313

Amino Acids

32.6

Weight (kDa)

4.73

Isoelectric Point (pI)

40.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 223
AasI GACNNNNNNGTC 1 cut(s) 221
Acc36I ACCTGC 1 cut(s) 223
AccBSI CCGCTC 1 cut(s) 869
AccI GTMKAC 3 cut(s) 101, 213, 225
AccII CGCG 1 cut(s) 209
AclWI GGATC 2 cut(s) 465, 478
AcoI YGGCCR 2 cut(s) 39, 630
AcsI RAATTY 1 cut(s) 607
AcuI CTGAAG 3 cut(s) 477, 564, 864
AcyI GRCGYC 1 cut(s) 354
AfiI CCNNNNNNNGG 1 cut(s) 364
AgsI TTSAA 6 cut(s) 319, 349, 515, 524, 567, 704
AluBI AGCT 7 cut(s) 315, 548, 686, 700, 729, 769, 836
AluI AGCT 7 cut(s) 315, 548, 686, 700, 729, 769, 836
Alw26I GTCTC 1 cut(s) 833
AlwI GGATC 2 cut(s) 465, 478
AoxI GGCC 5 cut(s) 39, 123, 324, 333, 630
ApeKI GCWGC 9 cut(s) 9, 140, 182, 305, 413, 416, 433, 668, 833
ApoI RAATTY 1 cut(s) 607
ArsI GACNNNNNNTTYG 2 cut(s) 94, 126
Asp700I GAANNNNTTC 1 cut(s) 818
AspLEI GCGC 3 cut(s) 85, 245, 310
AspS9I GGNCC 1 cut(s) 58
AsuC2I CCSGG 1 cut(s) 87
AsuHPI GGTGA 3 cut(s) 185, 274, 681
AsuII TTCGAA 1 cut(s) 822
AvaII GGWCC 1 cut(s) 58
AxyI CCTNAGG 1 cut(s) 459
BalI TGGCCA 1 cut(s) 632
BamHI GGATCC 1 cut(s) 470
BbsI GAAGAC 1 cut(s) 289
BbvI GCAGC 9 cut(s) 21, 152, 194, 292, 400, 403, 420, 655, 845
BccI CCATC 2 cut(s) 460, 595
BceAI ACGGC 4 cut(s) 79, 168, 181, 391
BcgI CGANNNNNNTGC 4 cut(s) 179, 213, 812, 846
BcnI CCSGG 1 cut(s) 87
BcoDI GTCTC 1 cut(s) 833
BfaI CTAG 1 cut(s) 486
BfmI CTRYAG 2 cut(s) 669, 831
BfoI RGCGCY 1 cut(s) 246
BfuAI ACCTGC 1 cut(s) 223
BglI GCCNNNNNGGC 1 cut(s) 332
Bme1390I CCNGG 1 cut(s) 87
Bme18I GGWCC 1 cut(s) 58
BmgT120I GGNCC 1 cut(s) 58
BmiI GGNNCC 3 cut(s) 93, 405, 472
BmrFI CCNGG 1 cut(s) 87
BmsI GCATC 2 cut(s) 273, 887
BpiI GAAGAC 1 cut(s) 289
Bpu14I TTCGAA 1 cut(s) 822
BpuMI CCSGG 1 cut(s) 87
BsaBI GATNNNNATC 1 cut(s) 678
BsaHI GRCGYC 1 cut(s) 354
BsaJI CCNNGG 5 cut(s) 61, 76, 86, 135, 287
BsaXI ACNNNNNCTCC 4 cut(s) 162, 192, 858, 888
Bsc4I CCNNNNNNNGG 1 cut(s) 364
Bse118I RCCGGY 1 cut(s) 41
Bse1I ACTGG 4 cut(s) 109, 233, 264, 900
Bse21I CCTNAGG 1 cut(s) 459
Bse8I GATNNNNATC 1 cut(s) 678
BseDI CCNNGG 5 cut(s) 61, 76, 86, 135, 287
BseGI GGATG 1 cut(s) 807
BseJI GATNNNNATC 1 cut(s) 678
BseLI CCNNNNNNNGG 1 cut(s) 364
BseMII CTCAG 5 cut(s) 170, 450, 521, 563, 828
BseNI ACTGG 4 cut(s) 109, 233, 264, 900
BseRI GAGGAG 1 cut(s) 476
BseXI GCAGC 9 cut(s) 21, 152, 194, 292, 400, 403, 420, 655, 845
BseYI CCCAGC 1 cut(s) 428
BsgI GTGCAG 1 cut(s) 776
Bsh1236I CGCG 1 cut(s) 209
Bsh1285I CGRYCG 2 cut(s) 189, 213
BshFI GGCC 5 cut(s) 41, 125, 326, 335, 632
BsiEI CGRYCG 2 cut(s) 189, 213
BsiSI CCGG 3 cut(s) 42, 86, 246
BslFI GGGAC 1 cut(s) 906
BslI CCNNNNNNNGG 1 cut(s) 364
BsmAI GTCTC 1 cut(s) 833
BsmFI GGGAC 1 cut(s) 906
BsmI GAATGC 1 cut(s) 598
BsnI GGCC 5 cut(s) 41, 125, 326, 335, 632
Bsp119I TTCGAA 1 cut(s) 822
Bsp143I GATC 2 cut(s) 186, 470
BspANI GGCC 5 cut(s) 41, 125, 326, 335, 632
BspCNI CTCAG 5 cut(s) 169, 451, 522, 562, 829
BspFNI CGCG 1 cut(s) 209
BspHI TCATGA 1 cut(s) 18
BspLI GGNNCC 3 cut(s) 93, 405, 472
BspMAI CTGCAG 2 cut(s) 673, 835
BspMI ACCTGC 1 cut(s) 223
BspPI GGATC 2 cut(s) 465, 478
BspQI GCTCTTC 1 cut(s) 516
BspT104I TTCGAA 1 cut(s) 822
BsrBI CCGCTC 1 cut(s) 869
BsrFI RCCGGY 1 cut(s) 41
BsrI ACTGG 4 cut(s) 109, 233, 264, 900
BssAI RCCGGY 1 cut(s) 41
BssECI CCNNGG 5 cut(s) 61, 76, 86, 135, 287
BssMI GATC 2 cut(s) 186, 470
BssNI GRCGYC 1 cut(s) 354
Bst4CI ACNGT 2 cut(s) 644, 775
Bst6I CTCTTC 2 cut(s) 516, 654
BstACI GRCGYC 1 cut(s) 354
BstBI TTCGAA 1 cut(s) 822
BstC8I GCNNGC 4 cut(s) 71, 198, 278, 933
BstDEI CTNAG 6 cut(s) 156, 459, 530, 549, 837, 936
BstDSI CCRYGG 3 cut(s) 61, 76, 135
BstENI CCTNNNNNAGG 1 cut(s) 362
BstF5I GGATG 1 cut(s) 807
BstFNI CGCG 1 cut(s) 209
BstH2I RGCGCY 1 cut(s) 246
BstHHI GCGC 3 cut(s) 85, 245, 310
BstKTI GATC 2 cut(s) 189, 473
BstMAI GTCTC 1 cut(s) 833
BstMBI GATC 2 cut(s) 186, 470
BstMCI CGRYCG 2 cut(s) 189, 213
BstMWI GCNNNNNNNGC 7 cut(s) 38, 206, 332, 422, 425, 515, 875
BstNSI RCATGY 2 cut(s) 73, 509
BstSCI CCNGG 1 cut(s) 85
BstSFI CTRYAG 2 cut(s) 669, 831
BstUI CGCG 1 cut(s) 209
BstV1I GCAGC 9 cut(s) 21, 152, 194, 292, 400, 403, 420, 655, 845
BstV2I GAAGAC 1 cut(s) 289
BstX2I RGATCY 1 cut(s) 470
BstXI CCANNNNNNTGG 1 cut(s) 588
BstYI RGATCY 1 cut(s) 470
Bsu36I CCTNAGG 1 cut(s) 459
BsuRI GGCC 5 cut(s) 41, 125, 326, 335, 632
BtgI CCRYGG 3 cut(s) 61, 76, 135
BtsCI GGATG 1 cut(s) 807
BtsIMutI CAGTG 1 cut(s) 501
BveI ACCTGC 1 cut(s) 223
Cac8I GCNNGC 4 cut(s) 71, 198, 278, 933
CciI TCATGA 1 cut(s) 18
CfoI GCGC 3 cut(s) 85, 245, 310
Cfr10I RCCGGY 1 cut(s) 41
Cfr13I GGNCC 1 cut(s) 58
CseI GACGC 4 cut(s) 189, 215, 311, 362
CspCI CAANNNNNGTGG 2 cut(s) 117, 152
CviAII CATG 4 cut(s) 19, 70, 506, 889
DdeI CTNAG 6 cut(s) 156, 459, 530, 549, 837, 936
DpnI GATC 2 cut(s) 188, 472
DpnII GATC 2 cut(s) 186, 470
DrdI GACNNNNNNGTC 1 cut(s) 221
DseDI GACNNNNNNGTC 1 cut(s) 221
EaeI YGGCCR 2 cut(s) 39, 630
Eam1104I CTCTTC 2 cut(s) 516, 654
EarI CTCTTC 2 cut(s) 516, 654
EciI GGCGGA 1 cut(s) 183
Eco147I AGGCCT 1 cut(s) 125
Eco47I GGWCC 1 cut(s) 58
Eco57I CTGAAG 3 cut(s) 477, 564, 864
Eco81I CCTNAGG 1 cut(s) 459
EcoNI CCTNNNNNAGG 1 cut(s) 362
FaeI CATG 4 cut(s) 22, 73, 509, 892
FaqI GGGAC 1 cut(s) 906
FatI CATG 4 cut(s) 18, 69, 505, 888
FblI GTMKAC 3 cut(s) 101, 213, 225
FokI GGATG 1 cut(s) 794
FspBI CTAG 1 cut(s) 486
GlaI GCGC 3 cut(s) 84, 244, 309
GsaI CCCAGC 1 cut(s) 432
HaeII RGCGCY 1 cut(s) 246
HaeIII GGCC 5 cut(s) 41, 125, 326, 335, 632
HapII CCGG 3 cut(s) 42, 86, 246
HgaI GACGC 4 cut(s) 189, 215, 311, 362
HhaI GCGC 3 cut(s) 85, 245, 310
Hin1I GRCGYC 1 cut(s) 354
Hin1II CATG 4 cut(s) 22, 73, 509, 892
Hin6I GCGC 3 cut(s) 83, 243, 308
HinP1I GCGC 3 cut(s) 83, 243, 308
HincII GTYRAC 3 cut(s) 102, 214, 226
HindII GTYRAC 3 cut(s) 102, 214, 226
HindIII AAGCTT 2 cut(s) 698, 767
HinfI GANTC 2 cut(s) 15, 710
HpaII CCGG 3 cut(s) 42, 86, 246
HphI GGTGA 3 cut(s) 185, 274, 681
Hpy166II GTNNAC 4 cut(s) 61, 102, 214, 226
Hpy188I TCNGA 6 cut(s) 159, 205, 441, 544, 552, 715
Hpy188III TCNNGA 5 cut(s) 19, 190, 589, 811, 922
Hpy8I GTNNAC 4 cut(s) 61, 102, 214, 226
Hpy99I CGWCG 4 cut(s) 205, 218, 227, 305
HpyCH4III ACNGT 2 cut(s) 644, 775
HpyCH4V TGCA 3 cut(s) 671, 757, 833
HpyF10VI GCNNNNNNNGC 7 cut(s) 38, 206, 332, 422, 425, 515, 875
HpyF3I CTNAG 6 cut(s) 156, 459, 530, 549, 837, 936
Hsp92I GRCGYC 1 cut(s) 354
Hsp92II CATG 4 cut(s) 22, 73, 509, 892
HspAI GCGC 3 cut(s) 83, 243, 308
Kzo9I GATC 2 cut(s) 186, 470
LguI GCTCTTC 1 cut(s) 516
LmnI GCTCC 3 cut(s) 78, 403, 866
Lsp1109I GCAGC 9 cut(s) 21, 152, 194, 292, 400, 403, 420, 655, 845
LweI GCATC 2 cut(s) 273, 887
MaeI CTAG 1 cut(s) 486
MaeIII GTNAC 1 cut(s) 575
MalI GATC 2 cut(s) 188, 472
MbiI CCGCTC 1 cut(s) 869
MboI GATC 2 cut(s) 186, 470
MboII GAAGA 7 cut(s) 263, 289, 369, 503, 671, 716, 746
MflI RGATCY 1 cut(s) 470
MlsI TGGCCA 1 cut(s) 632
MluCI AATT 5 cut(s) 129, 344, 567, 607, 623
MluNI TGGCCA 1 cut(s) 632
MlyI GAGTC 1 cut(s) 24
MmeI TCCRAC 1 cut(s) 183
Mox20I TGGCCA 1 cut(s) 632
MroXI GAANNNNTTC 1 cut(s) 818
MscI TGGCCA 1 cut(s) 632
MseI TTAA 1 cut(s) 651
Msp20I TGGCCA 1 cut(s) 632
MspA1I CMGCKG 3 cut(s) 12, 341, 836
MspI CCGG 3 cut(s) 42, 86, 246
MspR9I CCNGG 1 cut(s) 87
Mva1269I GAATGC 1 cut(s) 598
MvnI CGCG 1 cut(s) 209
MwoI GCNNNNNNNGC 7 cut(s) 38, 206, 332, 422, 425, 515, 875
NciI CCSGG 1 cut(s) 87
NdeII GATC 2 cut(s) 186, 470
NlaIII CATG 4 cut(s) 22, 73, 509, 892
NlaIV GGNNCC 3 cut(s) 93, 405, 472
NmeAIII GCCGAG 2 cut(s) 312, 336
NmuCI GTSAC 1 cut(s) 575
NspI RCATGY 2 cut(s) 73, 509
NspV TTCGAA 1 cut(s) 822
PaeI GCATGC 1 cut(s) 73
PagI TCATGA 1 cut(s) 18
PaqCI CACCTGC 1 cut(s) 223
PceI AGGCCT 1 cut(s) 125
PciSI GCTCTTC 1 cut(s) 516
PcsI WCGNNNNNNNCGW 3 cut(s) 213, 219, 222
PctI GAATGC 1 cut(s) 598
PdmI GAANNNNTTC 1 cut(s) 818
PfeI GAWTC 1 cut(s) 710
PflFI GACNNNGTC 2 cut(s) 209, 221
Ple19I CGATCG 1 cut(s) 189
PleI GAGTC 1 cut(s) 23
PpsI GAGTC 1 cut(s) 23
PspFI CCCAGC 1 cut(s) 428
PspN4I GGNNCC 3 cut(s) 93, 405, 472
PspPI GGNCC 1 cut(s) 58
PstI CTGCAG 2 cut(s) 673, 835
PsuI RGATCY 1 cut(s) 470
PsyI GACNNNGTC 2 cut(s) 209, 221
PvuI CGATCG 1 cut(s) 189
PvuII CAGCTG 1 cut(s) 836
SalI GTCGAC 3 cut(s) 100, 212, 224
SapI GCTCTTC 1 cut(s) 516
SaqAI TTAA 1 cut(s) 651
Sau3AI GATC 2 cut(s) 186, 470
Sau96I GGNCC 1 cut(s) 58
SchI GAGTC 1 cut(s) 24
ScrFI CCNGG 1 cut(s) 87
SfaNI GCATC 2 cut(s) 273, 887
SfcI CTRYAG 2 cut(s) 669, 831
SfiI GGCCNNNNNGGCC 1 cut(s) 332
SfuI TTCGAA 1 cut(s) 822
SinI GGWCC 1 cut(s) 58
SphI GCATGC 1 cut(s) 73
Sse9I AATT 5 cut(s) 129, 344, 567, 607, 623
SseBI AGGCCT 1 cut(s) 125
SspMI CTAG 1 cut(s) 486
StuI AGGCCT 1 cut(s) 125
StyD4I CCNGG 1 cut(s) 85
TaaI ACNGT 2 cut(s) 644, 775
TaqI TCGA 7 cut(s) 101, 189, 213, 225, 300, 360, 822
TasI AATT 5 cut(s) 129, 344, 567, 607, 623
TauI GCSGC 4 cut(s) 54, 69, 276, 428
TfiI GAWTC 1 cut(s) 710
Tru1I TTAA 1 cut(s) 651
Tru9I TTAA 1 cut(s) 651
TscAI CASTG 1 cut(s) 501
TseFI GTSAC 1 cut(s) 575
TseI GCWGC 9 cut(s) 9, 140, 182, 305, 413, 416, 433, 668, 833
Tsp45I GTSAC 1 cut(s) 575
TspDTI ATGAA 4 cut(s) 270, 723, 796, 877
TspGWI ACGGA 3 cut(s) 65, 671, 867
TspRI CASTG 1 cut(s) 501
Tth111I GACNNNGTC 2 cut(s) 209, 221
VpaK11BI GGWCC 1 cut(s) 58
XagI CCTNNNNNAGG 1 cut(s) 362
XapI RAATTY 1 cut(s) 607
XceI RCATGY 2 cut(s) 73, 509
XmiI GTMKAC 3 cut(s) 101, 213, 225
XmnI GAANNNNTTC 1 cut(s) 818
XspI CTAG 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.