pycom04g19980

Histone H1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0003009
Physical Location & Seq
Forward (+)
21572767 .. 21573535
769 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 618 bp
ATGACTGGAACTGGCGTCTCCGCTGCCAAGGCCAAGAAGGCGAGGTCTCCACCTGCACACCCGCCATTCGTCGAGATGATCACGGTGGCGATTGTGGCGCTGAAGGAGAGGACGGGGTCGAGCCAGTACGCGATCACCAAGTTTGTGGAGGAGAAGCACAAGAATCTGCCGCCGACCTTCAGGAAGCTTCTGCTCTACCATTTGAAGAAGCTCGTGGCTGCCGGAAAGCTCGTCAAGGTGAAAAACTCGTTCAAACTGCCTCCGGTCCGCGGCGCCGCTCCGGCGAAAGAGAAGACGGCCGGTGCGCCGAAGCCTAAGAAGTCGGCGGCGGTGGTCGCCAAGGCGAAGACCAAAACGGCTGCGAAGCCCAAGTCGAAAGGGGTGGCTGCTAAACCTAAGGCGGCAGCTGCGAAGCCAAAGGTGAAGAAGGCGGCGGCGGCCAAGCCTGCTGCCAAGGCGGCAAGAACATCGACGAGGACGTCGCCGGGGAAGAAGGTGGACGTGAAGTCTAAGGCTAAGGCGAAGAAGCCGGTGGCTCCGGCGAGAGCTGTGAGGAAGCCGAAGAGTGTGAAGTCTCCGGTGAAGAGGAAGGCTCCGGTGAGGAAGGCGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Linker_histone

Protein Analysis

206

Amino Acids

21.52

Weight (kDa)

11.52

Isoelectric Point (pI)

30.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Linker_histone PF00538 20 - 86 6.3e-18 linker histone H1 and H5 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 61
AasI GACNNNNNNGTC 1 cut(s) 478
AatII GACGTC 1 cut(s) 482
Acc36I ACCTGC 1 cut(s) 61
AccB1I GGYRCC 1 cut(s) 272
AccBSI CCGCTC 1 cut(s) 278
AccII CGCG 2 cut(s) 131, 270
AcoI YGGCCR 2 cut(s) 297, 438
AcuI CTGAAG 2 cut(s) 122, 163
AcyI GRCGYC 3 cut(s) 15, 273, 479
AfaI GTAC 1 cut(s) 128
AfiI CCNNNNNNNGG 1 cut(s) 269
AgsI TTSAA 2 cut(s) 205, 253
AhdI GACNNNNNGTC 1 cut(s) 505
AjiI CACGTC 1 cut(s) 502
AluBI AGCT 5 cut(s) 187, 211, 229, 407, 548
AluI AGCT 5 cut(s) 187, 211, 229, 407, 548
Alw26I GTCTC 3 cut(s) 22, 51, 579
AoxI GGCC 3 cut(s) 30, 297, 438
ApeKI GCWGC 7 cut(s) 23, 218, 359, 386, 404, 407, 449
ArsI GACNNNNNNTTYG 2 cut(s) 356, 388
AspLEI GCGC 3 cut(s) 100, 275, 307
AspS9I GGNCC 1 cut(s) 265
AsuC2I CCSGG 1 cut(s) 486
AsuHPI GGTGA 5 cut(s) 127, 250, 433, 592, 610
AvaII GGWCC 1 cut(s) 265
AxyI CCTNAGG 1 cut(s) 396
BanI GGYRCC 1 cut(s) 272
BauI CACGAG 1 cut(s) 212
BbsI GAAGAC 2 cut(s) 299, 353
BbvI GCAGC 7 cut(s) 10, 205, 346, 373, 394, 416, 436
BceAI ACGGC 2 cut(s) 312, 372
BcgI CGANNNNNNTGC 2 cut(s) 450, 484
BclI TGATCA 1 cut(s) 78
BcnI CCSGG 1 cut(s) 486
BcoDI GTCTC 3 cut(s) 22, 51, 579
BfoI RGCGCY 2 cut(s) 101, 276
BfuAI ACCTGC 1 cut(s) 61
BglI GCCNNNNNGGC 3 cut(s) 38, 281, 458
Bme1390I CCNGG 1 cut(s) 486
Bme18I GGWCC 1 cut(s) 265
BmeRI GACNNNNNGTC 1 cut(s) 505
BmgBI CACGTC 1 cut(s) 502
BmgT120I GGNCC 1 cut(s) 265
BmiI GGNNCC 3 cut(s) 274, 537, 594
BmrFI CCNGG 1 cut(s) 486
BpiI GAAGAC 2 cut(s) 299, 353
BplI GAGNNNNNCTC 2 cut(s) 577, 609
Bpu10I CCTNAGC 1 cut(s) 516
BpuMI CCSGG 1 cut(s) 486
BsaHI GRCGYC 3 cut(s) 15, 273, 479
BsaI GGTCTC 1 cut(s) 51
BsaJI CCNNGG 5 cut(s) 27, 268, 339, 453, 485
BsaWI WCCGGW 3 cut(s) 262, 577, 595
Bsc4I CCNNNNNNNGG 1 cut(s) 269
Bse118I RCCGGY 2 cut(s) 299, 529
Bse1I ACTGG 3 cut(s) 10, 16, 124
Bse21I CCTNAGG 1 cut(s) 396
BseDI CCNNGG 5 cut(s) 27, 268, 339, 453, 485
BseLI CCNNNNNNNGG 1 cut(s) 269
BseNI ACTGG 3 cut(s) 10, 16, 124
BseRI GAGGAG 1 cut(s) 164
BseX3I CGGCCG 1 cut(s) 297
BseXI GCAGC 7 cut(s) 10, 205, 346, 373, 394, 416, 436
BsgI GTGCAG 1 cut(s) 39
Bsh1236I CGCG 2 cut(s) 131, 270
Bsh1285I CGRYCG 1 cut(s) 300
BshFI GGCC 3 cut(s) 32, 299, 440
BshNI GGYRCC 1 cut(s) 272
BsiEI CGRYCG 1 cut(s) 300
BsiSI CCGG 9 cut(s) 222, 263, 281, 300, 485, 530, 539, 578, 596
BslI CCNNNNNNNGG 1 cut(s) 269
BsmAI GTCTC 3 cut(s) 22, 51, 579
BsmBI CGTCTC 1 cut(s) 22
BsnI GGCC 3 cut(s) 32, 299, 440
Bso31I GGTCTC 1 cut(s) 51
Bsp143I GATC 2 cut(s) 78, 132
BspANI GGCC 3 cut(s) 32, 299, 440
BspFNI CGCG 2 cut(s) 131, 270
BspLI GGNNCC 3 cut(s) 274, 537, 594
BspMI ACCTGC 1 cut(s) 61
BspT107I GGYRCC 1 cut(s) 272
BspTNI GGTCTC 1 cut(s) 51
BsrBI CCGCTC 1 cut(s) 278
BsrFI RCCGGY 2 cut(s) 299, 529
BsrI ACTGG 3 cut(s) 10, 16, 124
BssAI RCCGGY 2 cut(s) 299, 529
BssECI CCNNGG 5 cut(s) 27, 268, 339, 453, 485
BssMI GATC 2 cut(s) 78, 132
BssNI GRCGYC 3 cut(s) 15, 273, 479
BssSI CACGAG 1 cut(s) 212
BssT1I CCWWGG 3 cut(s) 27, 339, 453
Bst2BI CACGAG 1 cut(s) 212
Bst4CI ACNGT 1 cut(s) 85
Bst6I CTCTTC 2 cut(s) 557, 578
BstACI GRCGYC 3 cut(s) 15, 273, 479
BstC8I GCNNGC 1 cut(s) 447
BstDEI CTNAG 4 cut(s) 315, 396, 510, 516
BstDSI CCRYGG 1 cut(s) 268
BstFNI CGCG 2 cut(s) 131, 270
BstH2I RGCGCY 2 cut(s) 101, 276
BstHHI GCGC 3 cut(s) 100, 275, 307
BstKTI GATC 2 cut(s) 81, 135
BstMAI GTCTC 3 cut(s) 22, 51, 579
BstMBI GATC 2 cut(s) 78, 132
BstMCI CGRYCG 1 cut(s) 300
BstSCI CCNGG 1 cut(s) 484
BstUI CGCG 2 cut(s) 131, 270
BstV1I GCAGC 7 cut(s) 10, 205, 346, 373, 394, 416, 436
BstV2I GAAGAC 2 cut(s) 299, 353
BstXI CCANNNNNNTGG 1 cut(s) 145
BstZI CGGCCG 1 cut(s) 297
Bsu36I CCTNAGG 1 cut(s) 396
BsuRI GGCC 3 cut(s) 32, 299, 440
BtgI CCRYGG 1 cut(s) 268
BtrI CACGTC 1 cut(s) 502
BveI ACCTGC 1 cut(s) 61
Cac8I GCNNGC 1 cut(s) 447
CfoI GCGC 3 cut(s) 100, 275, 307
Cfr10I RCCGGY 2 cut(s) 299, 529
Cfr13I GGNCC 1 cut(s) 265
Cfr42I CCGCGG 1 cut(s) 271
CpoI CGGWCCG 1 cut(s) 265
CseI GACGC 1 cut(s) 4
Csp6I GTAC 1 cut(s) 127
CspI CGGWCCG 1 cut(s) 265
CviQI GTAC 1 cut(s) 127
DdeI CTNAG 4 cut(s) 315, 396, 510, 516
DinI GGCGCC 1 cut(s) 274
DpnI GATC 2 cut(s) 80, 134
DpnII GATC 2 cut(s) 78, 132
DrdI GACNNNNNNGTC 1 cut(s) 478
DriI GACNNNNNGTC 1 cut(s) 505
DseDI GACNNNNNNGTC 1 cut(s) 478
EaeI YGGCCR 2 cut(s) 297, 438
EagI CGGCCG 1 cut(s) 297
Eam1104I CTCTTC 2 cut(s) 557, 578
Eam1105I GACNNNNNGTC 1 cut(s) 505
EarI CTCTTC 2 cut(s) 557, 578
EclXI CGGCCG 1 cut(s) 297
Eco130I CCWWGG 3 cut(s) 27, 339, 453
Eco31I GGTCTC 1 cut(s) 51
Eco47I GGWCC 1 cut(s) 265
Eco52I CGGCCG 1 cut(s) 297
Eco57I CTGAAG 2 cut(s) 122, 163
Eco81I CCTNAGG 1 cut(s) 396
EcoT14I CCWWGG 3 cut(s) 27, 339, 453
EgeI GGCGCC 1 cut(s) 274
EheI GGCGCC 1 cut(s) 274
ErhI CCWWGG 3 cut(s) 27, 339, 453
Esp3I CGTCTC 1 cut(s) 22
FauI CCCGC 1 cut(s) 69
FbaI TGATCA 1 cut(s) 78
GlaI GCGC 3 cut(s) 99, 274, 306
HaeII RGCGCY 2 cut(s) 101, 276
HaeIII GGCC 3 cut(s) 32, 299, 440
HapII CCGG 9 cut(s) 222, 263, 281, 300, 485, 530, 539, 578, 596
HgaI GACGC 1 cut(s) 4
HhaI GCGC 3 cut(s) 100, 275, 307
Hin1I GRCGYC 3 cut(s) 15, 273, 479
Hin6I GCGC 3 cut(s) 98, 273, 305
HinP1I GCGC 3 cut(s) 98, 273, 305
HindIII AAGCTT 1 cut(s) 185
HinfI GANTC 1 cut(s) 163
HpaII CCGG 9 cut(s) 222, 263, 281, 300, 485, 530, 539, 578, 596
HphI GGTGA 5 cut(s) 127, 250, 433, 592, 610
Hpy166II GTNNAC 1 cut(s) 499
Hpy188III TCNNGA 2 cut(s) 73, 181
Hpy8I GTNNAC 1 cut(s) 499
Hpy99I CGWCG 3 cut(s) 74, 475, 484
HpyAV CCTTC 7 cut(s) 31, 97, 187, 421, 487, 583, 598
HpyCH4III ACNGT 1 cut(s) 85
HpyCH4IV ACGT 2 cut(s) 479, 501
HpyCH4V TGCA 1 cut(s) 56
HpyF3I CTNAG 4 cut(s) 315, 396, 510, 516
HpySE526I ACGT 2 cut(s) 479, 501
Hsp92I GRCGYC 3 cut(s) 15, 273, 479
HspAI GCGC 3 cut(s) 98, 273, 305
KasI GGCGCC 1 cut(s) 272
Ksp22I TGATCA 1 cut(s) 78
KspI CCGCGG 1 cut(s) 271
Kzo9I GATC 2 cut(s) 78, 132
LmnI GCTCC 3 cut(s) 283, 541, 598
Lsp1109I GCAGC 7 cut(s) 10, 205, 346, 373, 394, 416, 436
MaeII ACGT 2 cut(s) 479, 501
MalI GATC 2 cut(s) 80, 134
MbiI CCGCTC 1 cut(s) 278
MboI GATC 2 cut(s) 78, 132
MboII GAAGA 8 cut(s) 217, 304, 358, 436, 502, 535, 574, 595
Mly113I GGCGCC 1 cut(s) 273
MnlI CCTC 8 cut(s) 36, 102, 142, 270, 468, 546, 579, 594
MspA1I CMGCKG 3 cut(s) 23, 270, 407
MspI CCGG 9 cut(s) 222, 263, 281, 300, 485, 530, 539, 578, 596
MspR9I CCNGG 1 cut(s) 486
MvnI CGCG 2 cut(s) 131, 270
NarI GGCGCC 1 cut(s) 273
NciI CCSGG 1 cut(s) 486
NdeII GATC 2 cut(s) 78, 132
NlaIV GGNNCC 3 cut(s) 274, 537, 594
PaqCI CACCTGC 1 cut(s) 61
PcsI WCGNNNNNNNCGW 1 cut(s) 476
PfeI GAWTC 1 cut(s) 163
PflFI GACNNNGTC 1 cut(s) 115
PluTI GGCGCC 1 cut(s) 276
PspN4I GGNNCC 3 cut(s) 274, 537, 594
PspPI GGNCC 1 cut(s) 265
PsyI GACNNNGTC 1 cut(s) 115
PvuII CAGCTG 1 cut(s) 407
RsaI GTAC 1 cut(s) 128
RsaNI GTAC 1 cut(s) 127
Rsr2I CGGWCCG 1 cut(s) 265
RsrII CGGWCCG 1 cut(s) 265
SacII CCGCGG 1 cut(s) 271
Sau3AI GATC 2 cut(s) 78, 132
Sau96I GGNCC 1 cut(s) 265
ScrFI CCNGG 1 cut(s) 486
SfoI GGCGCC 1 cut(s) 274
Sfr303I CCGCGG 1 cut(s) 271
SgrBI CCGCGG 1 cut(s) 271
SinI GGWCC 1 cut(s) 265
SspDI GGCGCC 1 cut(s) 272
StyD4I CCNGG 1 cut(s) 484
StyI CCWWGG 3 cut(s) 27, 339, 453
TaaI ACNGT 1 cut(s) 85
TaiI ACGT 2 cut(s) 482, 504
TaqI TCGA 4 cut(s) 72, 119, 374, 470
TauI GCSGC 9 cut(s) 172, 273, 278, 329, 404, 434, 437, 440, 461
TfiI GAWTC 1 cut(s) 163
TseI GCWGC 7 cut(s) 23, 218, 359, 386, 404, 407, 449
Tth111I GACNNNGTC 1 cut(s) 115
VpaK11BI GGWCC 1 cut(s) 265
ZraI GACGTC 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.