pycom05g19050

Histone H1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003009
Physical Location & Seq
Reverse (-)
21959872 .. 21960438
567 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 447 bp
ATGGCGTCCGAAGAAGCAACCGTGGCCGTCGCGATCGAGACGGTCGAGAATCCTCCTCCCGAGCCGGTCACCGCCGAGACGGCGGACGACAAACCGGCAGACAATCCCGGTAAGGCCAGCAAGGCCAAGGAGCCCAAAGCGAAGAAGGCACCTGCTCCGAAGAAGCCGAGAGCCGCCCCCGCTCACCCTCCGTACGAAGAGATGGTTAAGGACGCAATTGTGGCTTTGAAGGAGAGGACTGGTTCGAGCCAGTACGCGATCACTAAGTTCATCGAGGAGAAGCACAAGCAGCTGCCGCCCAACTTCAAGAAGCTTCTGCTCTTCCATTTGAAGAAGCTTGTGAGTTCCGATAAGATCGATCAAGGTCAAAAACTCCTACAAGCTCCCGCCTGTGAGGTCCTCGGCGCCGAAGCCTTCTTCTCCGGTGAAGAAGAAACCGGCCGCTAA

Protein Analysis

149

Amino Acids

16.03

Weight (kDa)

6.85

Isoelectric Point (pI)

49.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Linker_histone PF00538 62 - 121 1.2e-17 linker histone H1 and H5 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 160
Acc36I ACCTGC 1 cut(s) 160
AccB1I GGYRCC 2 cut(s) 148, 404
AccBSI CCGCTC 1 cut(s) 182
AccII CGCG 2 cut(s) 32, 257
AciI CCGC 7 cut(s) 72, 83, 174, 180, 296, 387, 442
AcoI YGGCCR 2 cut(s) 24, 439
AcyI GRCGYC 2 cut(s) 5, 405
AfaI GTAC 2 cut(s) 194, 254
AgsI TTSAA 3 cut(s) 229, 307, 331
AluBI AGCT 4 cut(s) 292, 313, 337, 383
AluI AGCT 4 cut(s) 292, 313, 337, 383
Alw26I GTCTC 2 cut(s) 32, 71
Ama87I CYCGRG 1 cut(s) 59
AoxI GGCC 4 cut(s) 24, 114, 123, 439
ApeKI GCWGC 2 cut(s) 289, 292
AspLEI GCGC 1 cut(s) 407
AspS9I GGNCC 1 cut(s) 397
AsuC2I CCSGG 1 cut(s) 108
AsuHPI GGTGA 3 cut(s) 61, 176, 437
AvaI CYCGRG 1 cut(s) 59
AvaII GGWCC 1 cut(s) 397
BanI GGYRCC 2 cut(s) 148, 404
BanII GRGCYC 1 cut(s) 135
BbvI GCAGC 2 cut(s) 279, 301
BccI CCATC 1 cut(s) 196
BceAI ACGGC 2 cut(s) 11, 96
BcnI CCSGG 1 cut(s) 108
BcoDI GTCTC 2 cut(s) 32, 71
BfoI RGCGCY 1 cut(s) 408
BfuAI ACCTGC 1 cut(s) 160
BglI GCCNNNNNGGC 2 cut(s) 80, 122
BisI GCNGC 5 cut(s) 174, 290, 293, 296, 442
BlsI GCNGC 5 cut(s) 175, 291, 294, 297, 443
Bme1390I CCNGG 1 cut(s) 108
Bme18I GGWCC 1 cut(s) 397
BmeT110I CYCGRG 1 cut(s) 59
BmgT120I GGNCC 1 cut(s) 397
BmiI GGNNCC 3 cut(s) 132, 150, 406
BmrFI CCNGG 1 cut(s) 108
BpuMI CCSGG 1 cut(s) 108
Bsa29I ATCGAT 1 cut(s) 357
BsaHI GRCGYC 2 cut(s) 5, 405
BsaJI CCNNGG 3 cut(s) 21, 126, 400
BsaWI WCCGGW 1 cut(s) 422
Bse118I RCCGGY 3 cut(s) 64, 94, 437
Bse1I ACTGG 2 cut(s) 244, 250
BseCI ATCGAT 1 cut(s) 357
BseDI CCNNGG 3 cut(s) 21, 126, 400
BseNI ACTGG 2 cut(s) 244, 250
BseRI GAGGAG 2 cut(s) 45, 290
BseX3I CGGCCG 1 cut(s) 439
BseXI GCAGC 2 cut(s) 279, 301
Bsh1236I CGCG 2 cut(s) 32, 257
Bsh1285I CGRYCG 3 cut(s) 36, 45, 442
BshFI GGCC 4 cut(s) 26, 116, 125, 441
BshNI GGYRCC 2 cut(s) 148, 404
BshVI ATCGAT 1 cut(s) 357
BsiEI CGRYCG 3 cut(s) 36, 45, 442
BsiHKCI CYCGRG 1 cut(s) 59
BsiSI CCGG 5 cut(s) 65, 95, 108, 423, 438
BsiWI CGTACG 1 cut(s) 192
BsmAI GTCTC 2 cut(s) 32, 71
BsmBI CGTCTC 2 cut(s) 32, 71
BsnI GGCC 4 cut(s) 26, 116, 125, 441
BsoBI CYCGRG 1 cut(s) 59
Bsp1286I GDGCHC 1 cut(s) 135
Bsp143I GATC 4 cut(s) 33, 258, 354, 358
Bsp68I TCGCGA 1 cut(s) 32
BspACI CCGC 7 cut(s) 72, 83, 174, 180, 296, 387, 442
BspANI GGCC 4 cut(s) 26, 116, 125, 441
BspDI ATCGAT 1 cut(s) 357
BspFNI CGCG 2 cut(s) 32, 257
BspLI GGNNCC 3 cut(s) 132, 150, 406
BspMI ACCTGC 1 cut(s) 160
BspQI GCTCTTC 1 cut(s) 326
BspT107I GGYRCC 2 cut(s) 148, 404
BsrBI CCGCTC 1 cut(s) 182
BsrFI RCCGGY 3 cut(s) 64, 94, 437
BsrI ACTGG 2 cut(s) 244, 250
BssAI RCCGGY 3 cut(s) 64, 94, 437
BssECI CCNNGG 3 cut(s) 21, 126, 400
BssMI GATC 4 cut(s) 33, 258, 354, 358
BssNI GRCGYC 2 cut(s) 5, 405
BssT1I CCWWGG 1 cut(s) 126
Bst4CI ACNGT 2 cut(s) 22, 43
Bst6I CTCTTC 2 cut(s) 192, 326
BstACI GRCGYC 2 cut(s) 5, 405
BstC8I GCNNGC 1 cut(s) 118
BstDEI CTNAG 1 cut(s) 264
BstDSI CCRYGG 1 cut(s) 21
BstEII GGTNACC 1 cut(s) 67
BstFNI CGCG 2 cut(s) 32, 257
BstH2I RGCGCY 1 cut(s) 408
BstHHI GCGC 1 cut(s) 407
BstKTI GATC 4 cut(s) 36, 261, 357, 361
BstMAI GTCTC 2 cut(s) 32, 71
BstMBI GATC 4 cut(s) 33, 258, 354, 358
BstMCI CGRYCG 3 cut(s) 36, 45, 442
BstMWI GCNNNNNNNGC 8 cut(s) 23, 80, 122, 146, 179, 221, 289, 295
BstPI GGTNACC 1 cut(s) 67
BstSCI CCNGG 1 cut(s) 106
BstUI CGCG 2 cut(s) 32, 257
BstV1I GCAGC 2 cut(s) 279, 301
BstZI CGGCCG 1 cut(s) 439
Bsu15I ATCGAT 1 cut(s) 357
BsuRI GGCC 4 cut(s) 26, 116, 125, 441
BsuTUI ATCGAT 1 cut(s) 357
BtgI CCRYGG 1 cut(s) 21
BtuMI TCGCGA 1 cut(s) 32
BveI ACCTGC 1 cut(s) 160
Cac8I GCNNGC 1 cut(s) 118
CfoI GCGC 1 cut(s) 407
Cfr10I RCCGGY 3 cut(s) 64, 94, 437
Cfr13I GGNCC 1 cut(s) 397
ClaI ATCGAT 1 cut(s) 357
CseI GACGC 1 cut(s) 221
Csp6I GTAC 2 cut(s) 193, 253
CviQI GTAC 2 cut(s) 193, 253
DdeI CTNAG 1 cut(s) 264
DinI GGCGCC 1 cut(s) 406
DpnI GATC 4 cut(s) 35, 260, 356, 360
DpnII GATC 4 cut(s) 33, 258, 354, 358
EaeI YGGCCR 2 cut(s) 24, 439
EagI CGGCCG 1 cut(s) 439
Eam1104I CTCTTC 2 cut(s) 192, 326
EarI CTCTTC 2 cut(s) 192, 326
EciI GGCGGA 1 cut(s) 98
EclXI CGGCCG 1 cut(s) 439
Eco130I CCWWGG 1 cut(s) 126
Eco24I GRGCYC 1 cut(s) 135
Eco47I GGWCC 1 cut(s) 397
Eco52I CGGCCG 1 cut(s) 439
Eco88I CYCGRG 1 cut(s) 59
Eco91I GGTNACC 1 cut(s) 67
EcoO109I RGGNCCY 1 cut(s) 397
EcoO65I GGTNACC 1 cut(s) 67
EcoT14I CCWWGG 1 cut(s) 126
EcoT38I GRGCYC 1 cut(s) 135
EgeI GGCGCC 1 cut(s) 406
EheI GGCGCC 1 cut(s) 406
ErhI CCWWGG 1 cut(s) 126
Esp3I CGTCTC 2 cut(s) 32, 71
FauI CCCGC 2 cut(s) 187, 394
Fnu4HI GCNGC 5 cut(s) 174, 290, 293, 296, 442
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 5 cut(s) 174, 290, 293, 296, 442
GlaI GCGC 1 cut(s) 406
GluI GCNGC 5 cut(s) 174, 290, 293, 296, 442
HaeII RGCGCY 1 cut(s) 408
HaeIII GGCC 4 cut(s) 26, 116, 125, 441
HapII CCGG 5 cut(s) 65, 95, 108, 423, 438
HgaI GACGC 1 cut(s) 221
HhaI GCGC 1 cut(s) 407
Hin1I GRCGYC 2 cut(s) 5, 405
Hin6I GCGC 1 cut(s) 405
HinP1I GCGC 1 cut(s) 405
HindIII AAGCTT 2 cut(s) 311, 335
HinfI GANTC 1 cut(s) 49
HpaII CCGG 5 cut(s) 65, 95, 108, 423, 438
HphI GGTGA 3 cut(s) 61, 176, 437
Hpy188I TCNGA 3 cut(s) 10, 159, 349
Hpy188III TCNNGA 5 cut(s) 31, 37, 46, 59, 307
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 3 cut(s) 139, 223, 424
HpyCH4III ACNGT 2 cut(s) 22, 43
HpyF10VI GCNNNNNNNGC 8 cut(s) 23, 80, 122, 146, 179, 221, 289, 295
HpyF3I CTNAG 1 cut(s) 264
Hsp92I GRCGYC 2 cut(s) 5, 405
HspAI GCGC 1 cut(s) 405
KasI GGCGCC 1 cut(s) 404
Kzo9I GATC 4 cut(s) 33, 258, 354, 358
LguI GCTCTTC 1 cut(s) 326
LmnI GCTCC 3 cut(s) 130, 160, 388
LpnPI CCDG 9 cut(s) 78, 108, 121, 130, 165, 225, 263, 403, 436
Lsp1109I GCAGC 2 cut(s) 279, 301
MaeIII GTNAC 1 cut(s) 67
MalI GATC 4 cut(s) 35, 260, 356, 360
MbiI CCGCTC 1 cut(s) 182
MboI GATC 4 cut(s) 33, 258, 354, 358
MboII GAAGA 9 cut(s) 23, 154, 172, 209, 313, 343, 409, 440, 443
MfeI CAATTG 1 cut(s) 216
MhlI GDGCHC 1 cut(s) 135
MluCI AATT 1 cut(s) 216
Mly113I GGCGCC 1 cut(s) 405
MnlI CCTC 7 cut(s) 63, 66, 198, 228, 268, 388, 410
MseI TTAA 1 cut(s) 207
MspA1I CMGCKG 1 cut(s) 292
MspI CCGG 5 cut(s) 65, 95, 108, 423, 438
MspR9I CCNGG 1 cut(s) 108
MunI CAATTG 1 cut(s) 216
MvnI CGCG 2 cut(s) 32, 257
MwoI GCNNNNNNNGC 8 cut(s) 23, 80, 122, 146, 179, 221, 289, 295
NarI GGCGCC 1 cut(s) 405
NciI CCSGG 1 cut(s) 108
NdeII GATC 4 cut(s) 33, 258, 354, 358
NlaIV GGNNCC 3 cut(s) 132, 150, 406
NmeAIII GCCGAG 3 cut(s) 100, 192, 381
NmuCI GTSAC 1 cut(s) 67
NruI TCGCGA 1 cut(s) 32
PaqCI CACCTGC 1 cut(s) 160
PciSI GCTCTTC 1 cut(s) 326
PcsI WCGNNNNNNNCGW 1 cut(s) 42
PfeI GAWTC 1 cut(s) 49
Pfl23II CGTACG 1 cut(s) 192
PkrI GCNGC 5 cut(s) 175, 291, 294, 297, 443
Ple19I CGATCG 1 cut(s) 36
PluTI GGCGCC 1 cut(s) 408
PpuMI RGGWCCY 1 cut(s) 397
Psp5II RGGWCCY 1 cut(s) 397
PspEI GGTNACC 1 cut(s) 67
PspLI CGTACG 1 cut(s) 192
PspN4I GGNNCC 3 cut(s) 132, 150, 406
PspPI GGNCC 1 cut(s) 397
PspPPI RGGWCCY 1 cut(s) 397
PvuI CGATCG 1 cut(s) 36
PvuII CAGCTG 1 cut(s) 292
RruI TCGCGA 1 cut(s) 32
RsaI GTAC 2 cut(s) 194, 254
RsaNI GTAC 2 cut(s) 193, 253
SapI GCTCTTC 1 cut(s) 326
SaqAI TTAA 1 cut(s) 207
SatI GCNGC 5 cut(s) 174, 290, 293, 296, 442
Sau3AI GATC 4 cut(s) 33, 258, 354, 358
Sau96I GGNCC 1 cut(s) 397
ScrFI CCNGG 1 cut(s) 108
SduI GDGCHC 1 cut(s) 135
SetI ASST 7 cut(s) 154, 294, 315, 339, 367, 385, 399
SfiI GGCCNNNNNGGCC 1 cut(s) 122
SfoI GGCGCC 1 cut(s) 406
SinI GGWCC 1 cut(s) 397
Sse9I AATT 1 cut(s) 216
SsiI CCGC 7 cut(s) 72, 83, 174, 180, 296, 387, 442
SspDI GGCGCC 1 cut(s) 404
StyD4I CCNGG 1 cut(s) 106
StyI CCWWGG 1 cut(s) 126
TaaI ACNGT 2 cut(s) 22, 43
TaqI TCGA 5 cut(s) 36, 45, 245, 273, 357
TasI AATT 1 cut(s) 216
TauI GCSGC 3 cut(s) 176, 298, 444
TfiI GAWTC 1 cut(s) 49
Tru1I TTAA 1 cut(s) 207
Tru9I TTAA 1 cut(s) 207
TseFI GTSAC 1 cut(s) 67
TseI GCWGC 2 cut(s) 289, 292
Tsp45I GTSAC 1 cut(s) 67
TspDTI ATGAA 1 cut(s) 259
TspGWI ACGGA 1 cut(s) 180
VpaK11BI GGWCC 1 cut(s) 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.