pycom05g19490

Coiled-coil domain-containing protein 94 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0006725
Physical Location & Seq
Reverse (-)
22435149 .. 22437822
2674 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 810 bp
ATGGGAGCACGTAAGGTTCTTAACAAGTACTATCCGCCGGACTTTGACCCCTCGAAGCTGCCCAGGATCAGGAGGCCAAAGAATCAACAGATAAAGGTGCGGATGATGCTTCCGATGAGCATTCGCTGCAACACCTGCGGCAATTACATCTACAAAGGCACCAAGTTTAATTCTCGCAAAGAAGATGTTGTTGGAGAGACGTATTTGGGAATTCAAATATTCAGATTTTACTTCAAATGTACCAAGTGCTCTGCCGAGCTCGCGATGAAGACAGACCCCCAGAATTCTGACTATGTTGTTGAGGCCGGTGCGACACGGAATTTTGAACCCTGGCGCAATGAAGATGAGGAAGTAGATAAGGAGAAACAGAAAAGGGAGTCCGAAGAAATGGGAGATGCCATGAGAGCCTTGGAGAATAGAACACAGGATTCAAAAAGAGAGATGGACGTCATGGCTGCCTTGGATGAGATGAAGTCCATGAAGTCCAGGCATGCGACTGTGAGTGTGGATGCTATGTTGGAGGCTTTGAAACAAACATCCGCGCAAAAGGAAAAAAGGATTGAAGAGGAGGATGAAGCAGTCATAAAATCAATAGTATTCCATAACTCAAAAGATTATATCACAAGAATTCACGATGAAGATTTAGATGATGAAGAAGATATGATTGAGCCTTTAAGTGGCAACGGTGAAACCTCACTGAAGACCCTCAACTTGCAGCGGAAAAAGCTTACTCACGGGCGTCCTGACAATTCAACAGATTGGTTGACAAACAGCAGTATTTCTGATAGAGGTAGGCTCAGAAGCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

270

Amino Acids

31.21

Weight (kDa)

6.49

Isoelectric Point (pI)

44.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Saf4_Yju2 PF04502 9 - 234 1.2e-96 Saf4/Yju2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 143
AatII GACGTC 1 cut(s) 450
Acc36I ACCTGC 1 cut(s) 143
AccB1I GGYRCC 1 cut(s) 158
AccII CGCG 2 cut(s) 263, 542
AciI CCGC 5 cut(s) 35, 100, 138, 540, 718
AclWI GGATC 1 cut(s) 74
AcsI RAATTY 4 cut(s) 210, 283, 319, 627
AcuI CTGAAG 1 cut(s) 719
AcyI GRCGYC 2 cut(s) 447, 739
AfaI GTAC 2 cut(s) 29, 241
AfiI CCNNNNNNNGG 2 cut(s) 69, 677
AgsI TTSAA 7 cut(s) 215, 235, 326, 432, 529, 563, 753
AjnI CCWGG 3 cut(s) 62, 329, 485
AjuI GAANNNNNNNTTGG 2 cut(s) 174, 206
AluBI AGCT 4 cut(s) 58, 259, 727, 804
AluI AGCT 4 cut(s) 58, 259, 727, 804
Alw21I GWGCWC 3 cut(s) 10, 251, 261
Alw26I GTCTC 1 cut(s) 191
AlwI GGATC 1 cut(s) 74
AlwNI CAGNNNCTG 1 cut(s) 804
AoxI GGCC 2 cut(s) 74, 303
ApeKI GCWGC 5 cut(s) 58, 126, 455, 715, 804
ApoI RAATTY 4 cut(s) 210, 283, 319, 627
AspLEI GCGC 2 cut(s) 336, 544
AsuHPI GGTGA 1 cut(s) 698
BanI GGYRCC 1 cut(s) 158
BanII GRGCYC 1 cut(s) 261
BbsI GAAGAC 2 cut(s) 275, 707
Bbv12I GWGCWC 3 cut(s) 10, 251, 261
BbvI GCAGC 5 cut(s) 45, 113, 442, 727, 791
BccI CCATC 1 cut(s) 436
BciT130I CCWGG 3 cut(s) 64, 331, 487
BcoDI GTCTC 1 cut(s) 191
BfuAI ACCTGC 1 cut(s) 143
BisI GCNGC 6 cut(s) 59, 127, 139, 456, 716, 805
BlsI GCNGC 6 cut(s) 60, 128, 140, 457, 717, 806
BmcAI AGTACT 1 cut(s) 29
Bme1390I CCNGG 3 cut(s) 64, 331, 487
BmiI GGNNCC 1 cut(s) 160
BmrFI CCNGG 3 cut(s) 64, 331, 487
BmsI GCATC 3 cut(s) 96, 385, 499
BpiI GAAGAC 2 cut(s) 275, 707
BplI GAGNNNNNCTC 1 cut(s) 780
BsaAI YACGTR 1 cut(s) 11
BsaHI GRCGYC 2 cut(s) 447, 739
BsaJI CCNNGG 4 cut(s) 62, 329, 408, 459
Bsc4I CCNNNNNNNGG 2 cut(s) 69, 677
Bse118I RCCGGY 1 cut(s) 305
Bse3DI GCAATG 1 cut(s) 343
BseBI CCWGG 3 cut(s) 64, 331, 487
BseDI CCNNGG 4 cut(s) 62, 329, 408, 459
BseGI GGATG 5 cut(s) 108, 469, 514, 536, 577
BseLI CCNNNNNNNGG 2 cut(s) 69, 677
BseMI GCAATG 1 cut(s) 343
BseRI GAGGAG 1 cut(s) 581
BseXI GCAGC 5 cut(s) 45, 113, 442, 727, 791
Bsh1236I CGCG 2 cut(s) 263, 542
BshFI GGCC 2 cut(s) 76, 305
BshNI GGYRCC 1 cut(s) 158
BsiHKAI GWGCWC 3 cut(s) 10, 251, 261
BsiSI CCGG 2 cut(s) 38, 306
BslI CCNNNNNNNGG 2 cut(s) 69, 677
BsmAI GTCTC 1 cut(s) 191
BsmBI CGTCTC 1 cut(s) 191
BsmI GAATGC 1 cut(s) 120
BsnI GGCC 2 cut(s) 76, 305
Bsp1286I GDGCHC 3 cut(s) 10, 251, 261
Bsp143I GATC 1 cut(s) 66
Bsp68I TCGCGA 1 cut(s) 263
BspACI CCGC 5 cut(s) 35, 100, 138, 540, 718
BspANI GGCC 2 cut(s) 76, 305
BspCNI CTCAG 1 cut(s) 810
BspFNI CGCG 2 cut(s) 263, 542
BspLI GGNNCC 1 cut(s) 160
BspMI ACCTGC 1 cut(s) 143
BspPI GGATC 1 cut(s) 74
BspT107I GGYRCC 1 cut(s) 158
BsrDI GCAATG 1 cut(s) 343
BsrFI RCCGGY 1 cut(s) 305
BssAI RCCGGY 1 cut(s) 305
BssECI CCNNGG 4 cut(s) 62, 329, 408, 459
BssMI GATC 1 cut(s) 66
BssNI GRCGYC 2 cut(s) 447, 739
BssT1I CCWWGG 2 cut(s) 408, 459
Bst2UI CCWGG 3 cut(s) 64, 331, 487
Bst4CI ACNGT 2 cut(s) 499, 686
Bst6I CTCTTC 1 cut(s) 558
BstACI GRCGYC 2 cut(s) 447, 739
BstAPI GCANNNNNTGC 2 cut(s) 126, 135
BstBAI YACGTR 1 cut(s) 11
BstC8I GCNNGC 2 cut(s) 261, 492
BstDEI CTNAG 1 cut(s) 797
BstF5I GGATG 5 cut(s) 108, 469, 514, 536, 577
BstFNI CGCG 2 cut(s) 263, 542
BstHHI GCGC 2 cut(s) 336, 544
BstKTI GATC 1 cut(s) 69
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 1 cut(s) 66
BstMWI GCNNNNNNNGC 6 cut(s) 106, 126, 135, 260, 404, 724
BstNI CCWGG 3 cut(s) 64, 331, 487
BstNSI RCATGY 1 cut(s) 494
BstSCI CCNGG 3 cut(s) 62, 329, 485
BstUI CGCG 2 cut(s) 263, 542
BstV1I GCAGC 5 cut(s) 45, 113, 442, 727, 791
BstV2I GAAGAC 2 cut(s) 275, 707
BsuRI GGCC 2 cut(s) 76, 305
BtgZI GCGATG 1 cut(s) 278
BtsCI GGATG 5 cut(s) 108, 469, 514, 536, 577
BtsIMutI CAGTG 1 cut(s) 695
BtuMI TCGCGA 1 cut(s) 263
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 2 cut(s) 261, 492
CaiI CAGNNNCTG 1 cut(s) 804
CfoI GCGC 2 cut(s) 336, 544
Cfr10I RCCGGY 1 cut(s) 305
CseI GACGC 1 cut(s) 728
Csp6I GTAC 2 cut(s) 28, 240
CviAII CATG 4 cut(s) 400, 451, 478, 491
CviQI GTAC 2 cut(s) 28, 240
DdeI CTNAG 1 cut(s) 797
DpnI GATC 1 cut(s) 68
DpnII GATC 1 cut(s) 66
Eam1104I CTCTTC 1 cut(s) 558
EarI CTCTTC 1 cut(s) 558
EciI GGCGGA 1 cut(s) 24
Ecl136II GAGCTC 1 cut(s) 259
Eco130I CCWWGG 2 cut(s) 408, 459
Eco24I GRGCYC 1 cut(s) 261
Eco53kI GAGCTC 1 cut(s) 259
Eco57I CTGAAG 1 cut(s) 719
EcoICRI GAGCTC 1 cut(s) 259
EcoRI GAATTC 3 cut(s) 210, 283, 627
EcoRII CCWGG 3 cut(s) 62, 329, 485
EcoT14I CCWWGG 2 cut(s) 408, 459
EcoT38I GRGCYC 1 cut(s) 261
ErhI CCWWGG 2 cut(s) 408, 459
Esp3I CGTCTC 1 cut(s) 191
FaeI CATG 4 cut(s) 403, 454, 481, 494
FatI CATG 4 cut(s) 399, 450, 477, 490
Fnu4HI GCNGC 6 cut(s) 59, 127, 139, 456, 716, 805
FokI GGATG 5 cut(s) 115, 476, 521, 523, 584
FriOI GRGCYC 1 cut(s) 261
Fsp4HI GCNGC 6 cut(s) 59, 127, 139, 456, 716, 805
GlaI GCGC 2 cut(s) 335, 543
GluI GCNGC 6 cut(s) 59, 127, 139, 456, 716, 805
HaeIII GGCC 2 cut(s) 76, 305
HapII CCGG 2 cut(s) 38, 306
HgaI GACGC 1 cut(s) 728
HhaI GCGC 2 cut(s) 336, 544
Hin1I GRCGYC 2 cut(s) 447, 739
Hin1II CATG 4 cut(s) 403, 454, 481, 494
Hin6I GCGC 2 cut(s) 334, 542
HinP1I GCGC 2 cut(s) 334, 542
HincII GTYRAC 1 cut(s) 765
HindII GTYRAC 1 cut(s) 765
HindIII AAGCTT 1 cut(s) 725
HinfI GANTC 3 cut(s) 82, 377, 428
HpaII CCGG 2 cut(s) 38, 306
HphI GGTGA 1 cut(s) 698
Hpy166II GTNNAC 1 cut(s) 765
Hpy188I TCNGA 6 cut(s) 114, 224, 289, 382, 784, 800
Hpy188III TCNNGA 4 cut(s) 70, 262, 632, 743
Hpy8I GTNNAC 1 cut(s) 765
HpyCH4III ACNGT 2 cut(s) 499, 686
HpyCH4IV ACGT 3 cut(s) 10, 200, 447
HpyCH4V TGCA 2 cut(s) 129, 715
HpyF10VI GCNNNNNNNGC 6 cut(s) 106, 126, 135, 260, 404, 724
HpyF3I CTNAG 1 cut(s) 797
HpySE526I ACGT 3 cut(s) 10, 200, 447
Hsp92I GRCGYC 2 cut(s) 447, 739
Hsp92II CATG 4 cut(s) 403, 454, 481, 494
HspAI GCGC 2 cut(s) 334, 542
Kzo9I GATC 1 cut(s) 66
LmnI GCTCC 1 cut(s) 5
Lsp1109I GCAGC 5 cut(s) 45, 113, 442, 727, 791
LweI GCATC 3 cut(s) 96, 385, 499
MaeII ACGT 3 cut(s) 10, 200, 447
MalI GATC 1 cut(s) 68
MboI GATC 1 cut(s) 66
MboII GAAGA 9 cut(s) 194, 280, 353, 395, 575, 650, 665, 668, 712
MhlI GDGCHC 3 cut(s) 10, 251, 261
MluCI AATT 7 cut(s) 142, 169, 210, 283, 319, 627, 748
MlyI GAGTC 1 cut(s) 386
MmeI TCCRAC 2 cut(s) 172, 498
MseI TTAA 3 cut(s) 21, 168, 674
MspA1I CMGCKG 1 cut(s) 718
MspI CCGG 2 cut(s) 38, 306
MspR9I CCNGG 3 cut(s) 64, 331, 487
Mva1269I GAATGC 1 cut(s) 120
MvaI CCWGG 3 cut(s) 64, 331, 487
MvnI CGCG 2 cut(s) 263, 542
MwoI GCNNNNNNNGC 6 cut(s) 106, 126, 135, 260, 404, 724
NdeII GATC 1 cut(s) 66
NlaIII CATG 4 cut(s) 403, 454, 481, 494
NlaIV GGNNCC 1 cut(s) 160
NmeAIII GCCGAG 1 cut(s) 280
NruI TCGCGA 1 cut(s) 263
NspI RCATGY 1 cut(s) 494
PaeI GCATGC 1 cut(s) 494
PaqCI CACCTGC 1 cut(s) 143
PctI GAATGC 1 cut(s) 120
PfeI GAWTC 2 cut(s) 82, 428
PkrI GCNGC 6 cut(s) 60, 128, 140, 457, 717, 806
PleI GAGTC 1 cut(s) 385
PpsI GAGTC 1 cut(s) 385
Ppu21I YACGTR 1 cut(s) 11
Psp124BI GAGCTC 1 cut(s) 261
Psp6I CCWGG 3 cut(s) 62, 329, 485
PspGI CCWGG 3 cut(s) 62, 329, 485
PspN4I GGNNCC 1 cut(s) 160
PstNI CAGNNNCTG 1 cut(s) 804
RruI TCGCGA 1 cut(s) 263
RsaI GTAC 2 cut(s) 29, 241
RsaNI GTAC 2 cut(s) 28, 240
SacI GAGCTC 1 cut(s) 261
SaqAI TTAA 3 cut(s) 21, 168, 674
SatI GCNGC 6 cut(s) 59, 127, 139, 456, 716, 805
Sau3AI GATC 1 cut(s) 66
ScaI AGTACT 1 cut(s) 29
SchI GAGTC 1 cut(s) 386
ScrFI CCNGG 3 cut(s) 64, 331, 487
SduI GDGCHC 3 cut(s) 10, 251, 261
SfaNI GCATC 3 cut(s) 96, 385, 499
SphI GCATGC 1 cut(s) 494
Sse9I AATT 7 cut(s) 142, 169, 210, 283, 319, 627, 748
SsiI CCGC 5 cut(s) 35, 100, 138, 540, 718
SspI AATATT 1 cut(s) 219
SstI GAGCTC 1 cut(s) 261
StyD4I CCNGG 3 cut(s) 62, 329, 485
StyI CCWWGG 2 cut(s) 408, 459
TaaI ACNGT 2 cut(s) 499, 686
TaiI ACGT 3 cut(s) 13, 203, 450
TaqI TCGA 1 cut(s) 53
TasI AATT 7 cut(s) 142, 169, 210, 283, 319, 627, 748
TatI WGTACW 1 cut(s) 27
TauI GCSGC 1 cut(s) 141
TfiI GAWTC 2 cut(s) 82, 428
Tru1I TTAA 3 cut(s) 21, 168, 674
Tru9I TTAA 3 cut(s) 21, 168, 674
TscAI CASTG 1 cut(s) 702
TseI GCWGC 5 cut(s) 58, 126, 455, 715, 804
TspDTI ATGAA 7 cut(s) 281, 354, 485, 494, 588, 651, 666
TspGWI ACGGA 1 cut(s) 331
TspRI CASTG 1 cut(s) 702
XapI RAATTY 4 cut(s) 210, 283, 319, 627
XceI RCATGY 1 cut(s) 494
XcmI CCANNNNNNNNNTGG 1 cut(s) 406
ZraI GACGTC 1 cut(s) 448
ZrmI AGTACT 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.