pycom05g25550

Serine threonine-protein phosphatase 6 regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002321
Physical Location & Seq
Forward (+)
26903560 .. 26911092
7533 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2337 bp
ATGTTTTGGCGTATGGCGGGGTTGTCCACGGCGTCTCCGGTGGAGACAATTTTGGACAAGGATAATTTCACGTTGGAAGAGCTACTTGATGAAGATGAAATTATTCAGGAATGCAAAGCTCTTAATGGCCGCCTAATCAATTTTTTGCGAGAAAGAGCCCAGGTTGAGCAACTAATCCGATACATAGTTGAAGAAGCTCCTGAGGATGCTGAAAAGAGACGAACTTTCAAATTTCCTTTTATTGCGTGTGAGATTTTTACTTGTGAAGTTGATATCATACTCAAAACATTGGTAGAGGATGAGGAGTTGATGAACTTGTTGTTCTCCTTTTTGGAACCAAATAATTCTCACAGCACACTCTTGGCTGGGTACTTTAGCAAGGTTGTTGTTTGTCTGCTGTTGCGCAAGACAGTTCCTTTCCTGCAGTATATTCAGGGTCATCAAGATATTGTTAAGAAGCTAGTTGACCTGATTGGAATTACATCTATCATGGAGGTTTTGATACGTCTGGTTGGTGCTGATGAACACATGTATTCTAATCATACGGAGGCAATGCACTGGATACAAGATACAGATGTCCTAGAGATGATTGTGGACAAGTTCAGCTCTTCAGATTCTCCTGAAGTGCATGCTAATGCAGCAGAGACACTTTGTGCTATAACACGGTTTGCTCCGCCTGGACTTTCTTCTAAAATCTCCAGCCCAAGTTTTATAGGAAGATTGTTTCGGCATGCTTTGGAGGACTCACGGCCAAAATCTGTTCTTGTTAACTCGTTGTCAGTATGCATCTCTTTGCTAGACCCTAGGAGGCAAACTTTGGGAACATATCATATGTATGGCCGTCAGATGACTCATGGATCCACAGTTACTGCTAATCCTGAAACTGTTGTGGGCATGCTGGAGAGCCTAGGTGATTTGCTGAAGCTGTTGGATGTTTCATCGGTGGATAACACGCTGTTAACTACATATGGCAAGTTGCAGCCGCCTCTTGGAAAACATCGGTTAAAGGTTGTGGAGTTCATTTCAGTATTACTTACGGTTGGTAGTGAAGCTGCTGAGAAGGAATTGATTCGTCTTGGTGCTGTACAGAGAATTTTAGACTTGTTTTTTAAGTACCCATATAACAACTTTGTACATCACCACATAGAGAACATAATTGTGTCGTGTTTGGAAAGCAAGAATATTCCTCTTGTTGAACATCTTCTTCGTGAGTGTGACCTTGTTGGAAAAATACTTCAAGCAGAAAAGAATTTTACCTTGGCAGCTGACTCGGACAAGCCAACTGTTCCTGCAGAGGGTAGATCCCCACCCAGAATAGGGAATATTGGACATTTAACACGTATATCCAACAAACTCATTCAGCTTGGAAATAACAATAGCGAGATTCAGACGCTGCTGCAGGAGAACATCGAGTGGGCGGAGTGGCAGGCAAGTGTCCTGTCAAAGCGTAATACAGTCGAGAACGTCTATCAATGGGCCTGTGGGCGGCCAACTGCATTACATGATCGAATTAGGGATAGTGATGATGATGACTATCAAGATAGAGATTACGATGTTGCAGCTCTTGCAAATAACCTAAGCCAGGCTTTCCGATATGGAATATACAACAACGATGAGTTAGATGAGGGCCATGGCTCACTTGAACGGGATGATGAGGATGTCTACTTTGATGATGAATCTGCTGAAGTTGTTATATCTTCACTGCGTTTGGGAGATGACCAGGAAAGTGGTTCTCTGTTTACAAATTCCAACTGGTTTGCGTTTGAGGATGACAGGGCTGCCACTGATCGCTCAACTGGTTCTCTTGCTTCTCCATCACCAAATACGGTGGAGTCTAATGCTGTCAGTGGTGGCGGTGATGATGATGATGATGTCATTGTTGGTGAAGATGATGACTTGGTTGACACCGCAACATCTTCTCCAGAACCAGGAACAAAACCAGAAAATTCAGAAGTTGCCGTCCCCAACAATTCGCCAGAAGTGGGACCTGTTGAAACTGAAAAACCACCGGAGTGGGTTGAATGGAGGGAGAGTTCAGACTCTGGGGAACCATCTGATGTCCTACCAAATGGTGAGCTTCAAACGGAATCTGCAGATGCAGCTAAATGTTCTCCGTCCTCTTCTGAGCCACTGATAAAAGATGATAAAGTTGTTACTGAGCCTCAAGCTGAATCAACAGTTGAGGAAACTTCAAAGCCGCCTGAGCTGTCAGAACCAGGCAACGAGAAGCCAAGTTCTGAGTCAACCACTTCTGATGCTGCAGCTGCCGACGTGGGAACAAAAGGCAGTCAAGAAGCGGCTGCAGGTGATAAAGAGAAAGACAAGGAAGGGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAPS

Protein Analysis

779

Amino Acids

86.09

Weight (kDa)

4.49

Isoelectric Point (pI)

45.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAPS PF04499 131 - 489 8.4e-65 SIT4 phosphatase-associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2294
Acc16I TGCGCA 1 cut(s) 404
Acc36I ACCTGC 1 cut(s) 2294
AccI GTMKAC 1 cut(s) 1662
AclWI GGATC 3 cut(s) 852, 865, 1296
AcoI YGGCCR 4 cut(s) 127, 749, 838, 1487
AcsI RAATTY 5 cut(s) 230, 1092, 1249, 1744, 1945
AcuI CTGAAG 4 cut(s) 594, 642, 941, 1704
AcyI GRCGYC 1 cut(s) 32
AdeI CACNNNGTG 1 cut(s) 653
AfaI GTAC 4 cut(s) 371, 1086, 1115, 1134
AfiI CCNNNNNNNGG 8 cut(s) 989, 1295, 1316, 1317, 1485, 1582, 1928, 1982
AflIII ACRYGT 2 cut(s) 528, 1337
AgsI TTSAA 9 cut(s) 191, 229, 1196, 1238, 1643, 1994, 2021, 2081, 2193
AjiI CACGTC 1 cut(s) 2272
AjnI CCWGG 6 cut(s) 159, 676, 1581, 1719, 1927, 2215
AjuI GAANNNNNNNTTGG 2 cut(s) 1241, 1273
AleI CACNNNNGTG 1 cut(s) 2011
AloI GAACNNNNNNTCC 2 cut(s) 2017, 2049
Alw26I GTCTC 4 cut(s) 38, 39, 211, 638
AlwI GGATC 3 cut(s) 852, 865, 1296
AlwNI CAGNNNCTG 3 cut(s) 869, 1393, 2042
AoxI GGCC 6 cut(s) 127, 749, 838, 1476, 1487, 1627
ApoI RAATTY 5 cut(s) 230, 1092, 1249, 1744, 1945
Asp700I GAANNNNTTC 3 cut(s) 102, 1068, 1200
AspA2I CCTAGG 2 cut(s) 803, 907
AspLEI GCGC 1 cut(s) 405
AspS9I GGNCC 3 cut(s) 1476, 1627, 1985
AsuHPI GGTGA 7 cut(s) 923, 1130, 1809, 1868, 1895, 2084, 2318
AvaII GGWCC 1 cut(s) 1985
AvrII CCTAGG 2 cut(s) 803, 907
AxyI CCTNAGG 1 cut(s) 201
BaeI ACNNNNGTAYC 2 cut(s) 561, 594
BamHI GGATCC 1 cut(s) 857
BanII GRGCYC 1 cut(s) 160
BccI CCATC 2 cut(s) 1822, 2059
BceAI ACGGC 4 cut(s) 45, 764, 825, 1943
BcgI CGANNNNNNTGC 2 cut(s) 1251, 1285
BciT130I CCWGG 6 cut(s) 161, 678, 1583, 1721, 1929, 2217
BciVI GTATCC 1 cut(s) 555
BcoDI GTCTC 4 cut(s) 38, 39, 211, 638
BfaI CTAG 5 cut(s) 461, 581, 797, 804, 908
BfmI CTRYAG 6 cut(s) 422, 1290, 1397, 2091, 2259, 2301
BfuAI ACCTGC 1 cut(s) 2294
BfuI GTATCC 1 cut(s) 555
BlnI CCTAGG 2 cut(s) 803, 907
Bme1390I CCNGG 6 cut(s) 161, 678, 1583, 1721, 1929, 2217
Bme18I GGWCC 1 cut(s) 1985
BmgBI CACGTC 1 cut(s) 2272
BmgT120I GGNCC 3 cut(s) 1476, 1627, 1985
BmiI GGNNCC 4 cut(s) 336, 859, 1986, 2049
BmrFI CCNGG 6 cut(s) 161, 678, 1583, 1721, 1929, 2217
BmsI GCATC 4 cut(s) 196, 795, 2086, 2245
BpmI CTGGAG 3 cut(s) 682, 920, 1905
Bpu10I CCTNAGC 2 cut(s) 1577, 2202
BpuEI CTTGAG 1 cut(s) 2148
BsaAI YACGTR 1 cut(s) 1340
BsaBI GATNNNNATC 1 cut(s) 1533
BsaHI GRCGYC 1 cut(s) 32
BsaJI CCNNGG 6 cut(s) 27, 159, 803, 907, 1257, 1630
BsaWI WCCGGW 2 cut(s) 37, 2008
BsaXI ACNNNNNCTCC 6 cut(s) 19, 49, 1903, 1933, 2017, 2047
Bsc4I CCNNNNNNNGG 8 cut(s) 989, 1295, 1316, 1317, 1485, 1582, 1928, 1982
Bse1I ACTGG 3 cut(s) 563, 1757, 1801
Bse21I CCTNAGG 1 cut(s) 201
Bse3DI GCAATG 1 cut(s) 558
Bse8I GATNNNNATC 1 cut(s) 1533
BseBI CCWGG 6 cut(s) 161, 678, 1583, 1721, 1929, 2217
BseDI CCNNGG 6 cut(s) 27, 159, 803, 907, 1257, 1630
BseGI GGATG 6 cut(s) 211, 304, 937, 1654, 1663, 1774
BseJI GATNNNNATC 1 cut(s) 1533
BseLI CCNNNNNNNGG 8 cut(s) 989, 1295, 1316, 1317, 1485, 1582, 1928, 1982
BseMI GCAATG 1 cut(s) 558
BseMII CTCAG 6 cut(s) 192, 1047, 2115, 2148, 2193, 2229
BseNI ACTGG 3 cut(s) 563, 1757, 1801
BseRI GAGGAG 1 cut(s) 317
BseYI CCCAGC 1 cut(s) 365
BshFI GGCC 6 cut(s) 129, 751, 840, 1478, 1489, 1629
BsiSI CCGG 2 cut(s) 38, 2009
BslFI GGGAC 2 cut(s) 1946, 1998
BslI CCNNNNNNNGG 8 cut(s) 989, 1295, 1316, 1317, 1485, 1582, 1928, 1982
BsmAI GTCTC 4 cut(s) 38, 39, 211, 638
BsmBI CGTCTC 2 cut(s) 39, 211
BsmFI GGGAC 2 cut(s) 1946, 1998
BsmI GAATGC 1 cut(s) 116
BsnI GGCC 6 cut(s) 129, 751, 840, 1478, 1489, 1629
Bsp1286I GDGCHC 1 cut(s) 160
Bsp1407I TGTACA 2 cut(s) 1084, 1132
Bsp143I GATC 4 cut(s) 857, 1301, 1504, 1786
Bsp19I CCATGG 1 cut(s) 1630
BspANI GGCC 6 cut(s) 129, 751, 840, 1478, 1489, 1629
BspCNI CTCAG 6 cut(s) 193, 1048, 2116, 2149, 2194, 2230
BspLI GGNNCC 4 cut(s) 336, 859, 1986, 2049
BspMAI CTGCAG 6 cut(s) 426, 1294, 1401, 2095, 2263, 2305
BspMI ACCTGC 1 cut(s) 2294
BspPI GGATC 3 cut(s) 852, 865, 1296
BspQI GCTCTTC 2 cut(s) 72, 613
BsrDI GCAATG 1 cut(s) 558
BsrGI TGTACA 2 cut(s) 1084, 1132
BsrI ACTGG 3 cut(s) 563, 1757, 1801
BssECI CCNNGG 6 cut(s) 27, 159, 803, 907, 1257, 1630
BssMI GATC 4 cut(s) 857, 1301, 1504, 1786
BssNI GRCGYC 1 cut(s) 32
BssT1I CCWWGG 4 cut(s) 803, 907, 1257, 1630
Bst2UI CCWGG 6 cut(s) 161, 678, 1583, 1721, 1929, 2217
Bst4CI ACNGT 9 cut(s) 412, 666, 865, 886, 1039, 1285, 1456, 1828, 2179
Bst6I CTCTTC 3 cut(s) 72, 613, 2125
BstACI GRCGYC 1 cut(s) 32
BstAPI GCANNNNNTGC 1 cut(s) 1565
BstAUI TGTACA 2 cut(s) 1084, 1132
BstBAI YACGTR 1 cut(s) 1340
BstC8I GCNNGC 4 cut(s) 630, 732, 896, 1428
BstDEI CTNAG 7 cut(s) 201, 1056, 1577, 2124, 2157, 2202, 2238
BstDSI CCRYGG 2 cut(s) 27, 1630
BstENI CCTNNNNNAGG 1 cut(s) 1580
BstF5I GGATG 6 cut(s) 211, 304, 937, 1654, 1663, 1774
BstHHI GCGC 1 cut(s) 405
BstKTI GATC 4 cut(s) 860, 1304, 1507, 1789
BstMAI GTCTC 4 cut(s) 38, 39, 211, 638
BstMBI GATC 4 cut(s) 857, 1301, 1504, 1786
BstMWI GCNNNNNNNGC 5 cut(s) 638, 1565, 2099, 2203, 2264
BstNI CCWGG 6 cut(s) 161, 678, 1583, 1721, 1929, 2217
BstNSI RCATGY 4 cut(s) 532, 632, 734, 898
BstSCI CCNGG 6 cut(s) 159, 676, 1581, 1719, 1927, 2215
BstSFI CTRYAG 6 cut(s) 422, 1290, 1397, 2091, 2259, 2301
BstX2I RGATCY 2 cut(s) 857, 1301
BstXI CCANNNNNNTGG 2 cut(s) 1727, 2013
BstYI RGATCY 2 cut(s) 857, 1301
Bsu36I CCTNAGG 1 cut(s) 201
BsuI GTATCC 1 cut(s) 555
BsuRI GGCC 6 cut(s) 129, 751, 840, 1478, 1489, 1629
BtgI CCRYGG 2 cut(s) 27, 1630
BtrI CACGTC 1 cut(s) 2272
BtsCI GGATG 6 cut(s) 211, 304, 937, 1654, 1663, 1774
BtsI GCAGTG 1 cut(s) 1700
BtsIMutI CAGTG 5 cut(s) 556, 1700, 1782, 1852, 2129
BveI ACCTGC 1 cut(s) 2294
Cac8I GCNNGC 4 cut(s) 630, 732, 896, 1428
CaiI CAGNNNCTG 3 cut(s) 869, 1393, 2042
CfoI GCGC 1 cut(s) 405
Cfr13I GGNCC 3 cut(s) 1476, 1627, 1985
CseI GACGC 2 cut(s) 21, 1399
Csp6I GTAC 4 cut(s) 370, 1085, 1114, 1133
CspCI CAANNNNNGTGG 2 cut(s) 1809, 1844
CviAII CATG 8 cut(s) 490, 529, 629, 731, 854, 895, 1502, 1631
CviQI GTAC 4 cut(s) 370, 1085, 1114, 1133
DdeI CTNAG 7 cut(s) 201, 1056, 1577, 2124, 2157, 2202, 2238
DpnI GATC 4 cut(s) 859, 1303, 1506, 1788
DpnII GATC 4 cut(s) 857, 1301, 1504, 1786
DraIII CACNNNGTG 1 cut(s) 653
EaeI YGGCCR 4 cut(s) 127, 749, 838, 1487
Eam1104I CTCTTC 3 cut(s) 72, 613, 2125
EarI CTCTTC 3 cut(s) 72, 613, 2125
EciI GGCGGA 2 cut(s) 663, 1433
Eco130I CCWWGG 4 cut(s) 803, 907, 1257, 1630
Eco24I GRGCYC 1 cut(s) 160
Eco32I GATATC 1 cut(s) 274
Eco47I GGWCC 1 cut(s) 1985
Eco57I CTGAAG 4 cut(s) 594, 642, 941, 1704
Eco81I CCTNAGG 1 cut(s) 201
EcoNI CCTNNNNNAGG 1 cut(s) 1580
EcoO109I RGGNCCY 1 cut(s) 1985
EcoRII CCWGG 6 cut(s) 159, 676, 1581, 1719, 1927, 2215
EcoRV GATATC 1 cut(s) 274
EcoT14I CCWWGG 4 cut(s) 803, 907, 1257, 1630
EcoT22I ATGCAT 1 cut(s) 788
EcoT38I GRGCYC 1 cut(s) 160
ErhI CCWWGG 4 cut(s) 803, 907, 1257, 1630
Esp3I CGTCTC 2 cut(s) 39, 211
FaeI CATG 8 cut(s) 493, 532, 632, 734, 857, 898, 1505, 1634
FalI AAGNNNNNCTT 4 cut(s) 69, 101, 1570, 1602
FaqI GGGAC 2 cut(s) 1946, 1998
FatI CATG 8 cut(s) 489, 528, 628, 730, 853, 894, 1501, 1630
FauI CCCGC 1 cut(s) 10
FauNDI CATATG 2 cut(s) 831, 967
FblI GTMKAC 1 cut(s) 1662
FokI GGATG 6 cut(s) 218, 311, 944, 1661, 1670, 1781
FriOI GRGCYC 1 cut(s) 160
FspBI CTAG 5 cut(s) 461, 581, 797, 804, 908
FspI TGCGCA 1 cut(s) 404
GlaI GCGC 1 cut(s) 404
GsaI CCCAGC 1 cut(s) 369
GsuI CTGGAG 3 cut(s) 682, 920, 1905
HaeIII GGCC 6 cut(s) 129, 751, 840, 1478, 1489, 1629
HapII CCGG 2 cut(s) 38, 2009
HgaI GACGC 2 cut(s) 21, 1399
HhaI GCGC 1 cut(s) 405
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 8 cut(s) 493, 532, 632, 734, 857, 898, 1505, 1634
Hin6I GCGC 1 cut(s) 403
HinP1I GCGC 1 cut(s) 403
HincII GTYRAC 5 cut(s) 466, 769, 960, 1903, 2244
HindII GTYRAC 5 cut(s) 466, 769, 960, 1903, 2244
HpaI GTTAAC 2 cut(s) 769, 960
HpaII CCGG 2 cut(s) 38, 2009
HphI GGTGA 7 cut(s) 923, 1130, 1809, 1868, 1895, 2084, 2318
Hpy166II GTNNAC 9 cut(s) 27, 466, 595, 769, 960, 1663, 1740, 1903, 2244
Hpy8I GTNNAC 9 cut(s) 27, 466, 595, 769, 960, 1663, 1740, 1903, 2244
Hpy99I CGWCG 1 cut(s) 2273
HpyAV CCTTC 2 cut(s) 1054, 2321
HpyCH4III ACNGT 9 cut(s) 412, 666, 865, 886, 1039, 1285, 1456, 1828, 2179
HpyCH4IV ACGT 5 cut(s) 71, 505, 1339, 1464, 2271
HpyF10VI GCNNNNNNNGC 5 cut(s) 638, 1565, 2099, 2203, 2264
HpyF3I CTNAG 7 cut(s) 201, 1056, 1577, 2124, 2157, 2202, 2238
HpySE526I ACGT 5 cut(s) 71, 505, 1339, 1464, 2271
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 8 cut(s) 493, 532, 632, 734, 857, 898, 1505, 1634
HspAI GCGC 1 cut(s) 403
KspAI GTTAAC 2 cut(s) 769, 960
Kzo9I GATC 4 cut(s) 857, 1301, 1504, 1786
LguI GCTCTTC 2 cut(s) 72, 613
LmnI GCTCC 2 cut(s) 202, 676
LweI GCATC 4 cut(s) 196, 795, 2086, 2245
MaeI CTAG 5 cut(s) 461, 581, 797, 804, 908
MaeII ACGT 5 cut(s) 71, 505, 1339, 1464, 2271
MaeIII GTNAC 3 cut(s) 865, 1214, 2152
MalI GATC 4 cut(s) 859, 1303, 1506, 1788
MboI GATC 4 cut(s) 857, 1301, 1504, 1786
MflI RGATCY 2 cut(s) 857, 1301
MhlI GDGCHC 1 cut(s) 160
MlyI GAGTC 6 cut(s) 737, 844, 1262, 1841, 2033, 2249
MmeI TCCRAC 5 cut(s) 54, 909, 1204, 1371, 1773
Mph1103I ATGCAT 1 cut(s) 788
MroXI GAANNNNTTC 3 cut(s) 102, 1068, 1200
MseI TTAA 7 cut(s) 123, 453, 768, 959, 1004, 1110, 1334
MslI CAYNNNNRTG 4 cut(s) 633, 834, 1157, 2011
MspA1I CMGCKG 2 cut(s) 1265, 2264
MspI CCGG 2 cut(s) 38, 2009
MspR9I CCNGG 6 cut(s) 161, 678, 1583, 1721, 1929, 2217
Mva1269I GAATGC 1 cut(s) 116
MvaI CCWGG 6 cut(s) 161, 678, 1583, 1721, 1929, 2217
MwoI GCNNNNNNNGC 5 cut(s) 638, 1565, 2099, 2203, 2264
NcoI CCATGG 1 cut(s) 1630
NdeI CATATG 2 cut(s) 831, 967
NdeII GATC 4 cut(s) 857, 1301, 1504, 1786
NlaIII CATG 8 cut(s) 493, 532, 632, 734, 857, 898, 1505, 1634
NlaIV GGNNCC 4 cut(s) 336, 859, 1986, 2049
NmuCI GTSAC 1 cut(s) 1214
NsbI TGCGCA 1 cut(s) 404
NsiI ATGCAT 1 cut(s) 788
NspI RCATGY 4 cut(s) 532, 632, 734, 898
OliI CACNNNNGTG 1 cut(s) 2011
PaeI GCATGC 3 cut(s) 632, 734, 898
PaqCI CACCTGC 1 cut(s) 2294
PciI ACATGT 1 cut(s) 528
PciSI GCTCTTC 2 cut(s) 72, 613
PctI GAATGC 1 cut(s) 116
PdmI GAANNNNTTC 3 cut(s) 102, 1068, 1200
PfeI GAWTC 6 cut(s) 614, 1069, 1384, 1676, 2087, 2171
PleI GAGTC 6 cut(s) 737, 844, 1262, 1840, 2033, 2248
PpsI GAGTC 6 cut(s) 737, 844, 1262, 1840, 2033, 2248
Ppu21I YACGTR 1 cut(s) 1340
PpuMI RGGWCCY 1 cut(s) 1985
PscI ACATGT 1 cut(s) 528
Psp5II RGGWCCY 1 cut(s) 1985
Psp6I CCWGG 6 cut(s) 159, 676, 1581, 1719, 1927, 2215
PspFI CCCAGC 1 cut(s) 365
PspGI CCWGG 6 cut(s) 159, 676, 1581, 1719, 1927, 2215
PspN4I GGNNCC 4 cut(s) 336, 859, 1986, 2049
PspPI GGNCC 3 cut(s) 1476, 1627, 1985
PspPPI RGGWCCY 1 cut(s) 1985
PstI CTGCAG 6 cut(s) 426, 1294, 1401, 2095, 2263, 2305
PstNI CAGNNNCTG 3 cut(s) 869, 1393, 2042
PsuI RGATCY 2 cut(s) 857, 1301
PvuII CAGCTG 2 cut(s) 1265, 2264
RsaI GTAC 4 cut(s) 371, 1086, 1115, 1134
RsaNI GTAC 4 cut(s) 370, 1085, 1114, 1133
RseI CAYNNNNRTG 4 cut(s) 633, 834, 1157, 2011
SapI GCTCTTC 2 cut(s) 72, 613
SaqAI TTAA 7 cut(s) 123, 453, 768, 959, 1004, 1110, 1334
Sau3AI GATC 4 cut(s) 857, 1301, 1504, 1786
Sau96I GGNCC 3 cut(s) 1476, 1627, 1985
SchI GAGTC 6 cut(s) 737, 844, 1262, 1841, 2033, 2249
ScrFI CCNGG 6 cut(s) 161, 678, 1583, 1721, 1929, 2217
SduI GDGCHC 1 cut(s) 160
SfaNI GCATC 4 cut(s) 196, 795, 2086, 2245
SfcI CTRYAG 6 cut(s) 422, 1290, 1397, 2091, 2259, 2301
SinI GGWCC 1 cut(s) 1985
SmiMI CAYNNNNRTG 4 cut(s) 633, 834, 1157, 2011
SmlI CTYRAG 1 cut(s) 2163
SmoI CTYRAG 1 cut(s) 2163
SphI GCATGC 3 cut(s) 632, 734, 898
SspI AATATT 2 cut(s) 1183, 1324
SspMI CTAG 5 cut(s) 461, 581, 797, 804, 908
StyD4I CCNGG 6 cut(s) 159, 676, 1581, 1719, 1927, 2215
StyI CCWWGG 4 cut(s) 803, 907, 1257, 1630
TaaI ACNGT 9 cut(s) 412, 666, 865, 886, 1039, 1285, 1456, 1828, 2179
TaiI ACGT 5 cut(s) 74, 508, 1342, 1467, 2274
TaqI TCGA 3 cut(s) 1410, 1458, 1507
TatI WGTACW 2 cut(s) 1084, 1132
TauI GCSGC 5 cut(s) 132, 985, 1489, 2200, 2300
TfiI GAWTC 6 cut(s) 614, 1069, 1384, 1676, 2087, 2171
Tru1I TTAA 7 cut(s) 123, 453, 768, 959, 1004, 1110, 1334
Tru9I TTAA 7 cut(s) 123, 453, 768, 959, 1004, 1110, 1334
TscAI CASTG 5 cut(s) 563, 1707, 1789, 1852, 2136
TseFI GTSAC 1 cut(s) 1214
Tsp45I GTSAC 1 cut(s) 1214
TspDTI ATGAA 7 cut(s) 105, 111, 326, 537, 927, 1009, 1689
TspGWI ACGGA 3 cut(s) 560, 2099, 2103
TspRI CASTG 5 cut(s) 563, 1707, 1789, 1852, 2136
VpaK11BI GGWCC 1 cut(s) 1985
XagI CCTNNNNNAGG 1 cut(s) 1580
XapI RAATTY 5 cut(s) 230, 1092, 1249, 1744, 1945
XceI RCATGY 4 cut(s) 532, 632, 734, 898
XmaJI CCTAGG 2 cut(s) 803, 907
XmiI GTMKAC 1 cut(s) 1662
XmnI GAANNNNTTC 3 cut(s) 102, 1068, 1200
XspI CTAG 5 cut(s) 461, 581, 797, 804, 908
Zsp2I ATGCAT 1 cut(s) 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.