pycom05g26570
MADS Family

Agamous-like MADS-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003506
Physical Location & Seq
Reverse (-)
27784193 .. 27784828
636 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g26570.1

Sequence Viewer

Length: 636 bp
ATGGCCATGGAGGGAAAGCAAACTAGAGGTAGGCAAAAGATTGAGATGAAGAGGATCGAAAACGAAGAGGATCTTCTTGTTTCTTTCTCGAAACGGAGATCCGGCGTCTTCAAAAAAGCTAGCGAGATGATAACACTATGTGGCGGTGAGGTGGGAATTGTGGTTTTCTCACCATCTGGCAAGCCTTTCTCGTATGGTCATCCATCTGTCGACAATGTCATCAATCGGTTTCTGGACCAAAATCCACCCGAAAACGATGACTACACTATTCAGTTCATGGAGAATCATCGACAGATGAGAATTTCCGAGGCTATCGAACAGTACAACGAGCTTATCAACCAGTTAGAGGATGTGCAGGAGCGACAGAAATCGCTCCTAAGGAAAGCCAGGAGAAGGAGGAATCGAGAGCACCTGTGGTGGAGTGCACCCCTTGATGAGCTTGGGGTGGATGAGCTCAAACAAGTCTATGTATCCTCGGCGGAGATACACATGACAATCTCCAACCATCTTAAGGAGAGGAGTACCAATATTTTTGGTGCCTCCTCTTCTGGATCTGTCCAACAAACCAATCCCAACTTTGATGCAAATCCAAGTGGGACTGGTTCTTATGCATTTCCTCCTGGCTACGGCCATTAA

Protein Analysis

212

Amino Acids

24.09

Weight (kDa)

6.61

Isoelectric Point (pI)

54.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 18 - 65 5.5e-20 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 536
AccI GTMKAC 1 cut(s) 210
AciI CCGC 2 cut(s) 144, 479
AclWI GGATC 4 cut(s) 62, 78, 93, 559
AcoI YGGCCR 2 cut(s) 3, 628
AcsI RAATTY 1 cut(s) 300
AcyI GRCGYC 1 cut(s) 105
AdeI CACNNNGTG 1 cut(s) 140
AfaI GTAC 2 cut(s) 323, 523
AfiI CCNNNNNNNGG 4 cut(s) 346, 393, 511, 626
AflII CTTAAG 1 cut(s) 509
AgsI TTSAA 1 cut(s) 112
AjnI CCWGG 2 cut(s) 386, 619
AluBI AGCT 4 cut(s) 119, 331, 439, 454
AluI AGCT 4 cut(s) 119, 331, 439, 454
Alw21I GWGCWC 3 cut(s) 411, 427, 456
Alw44I GTGCAC 1 cut(s) 423
AlwI GGATC 4 cut(s) 62, 78, 93, 559
AoxI GGCC 2 cut(s) 3, 628
ApaLI GTGCAC 1 cut(s) 423
ApoI RAATTY 1 cut(s) 300
AspS9I GGNCC 1 cut(s) 235
AsuHPI GGTGA 2 cut(s) 158, 162
AsuNHI GCTAGC 1 cut(s) 119
AvaII GGWCC 1 cut(s) 235
AxyI CCTNAGG 1 cut(s) 377
BaeGI GKGCMC 1 cut(s) 427
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 536
BanII GRGCYC 1 cut(s) 456
BbsI GAAGAC 1 cut(s) 100
Bbv12I GWGCWC 3 cut(s) 411, 427, 456
BccI CCATC 3 cut(s) 181, 211, 513
BciT130I CCWGG 2 cut(s) 388, 621
BciVI GTATCC 1 cut(s) 481
BfaI CTAG 2 cut(s) 24, 120
BfrI CTTAAG 1 cut(s) 509
BfuI GTATCC 1 cut(s) 481
Bme1390I CCNGG 2 cut(s) 388, 621
Bme18I GGWCC 1 cut(s) 235
BmgT120I GGNCC 1 cut(s) 235
BmiI GGNNCC 1 cut(s) 538
BmrFI CCNGG 2 cut(s) 388, 621
BmsI GCATC 1 cut(s) 571
BmtI GCTAGC 1 cut(s) 123
BpiI GAAGAC 1 cut(s) 100
BsaBI GATNNNNATC 1 cut(s) 585
BsaHI GRCGYC 1 cut(s) 105
BsaJI CCNNGG 3 cut(s) 6, 306, 474
Bsc4I CCNNNNNNNGG 4 cut(s) 346, 393, 511, 626
Bse1I ACTGG 2 cut(s) 340, 604
Bse21I CCTNAGG 1 cut(s) 377
Bse8I GATNNNNATC 1 cut(s) 585
BseBI CCWGG 2 cut(s) 388, 621
BseDI CCNNGG 3 cut(s) 6, 306, 474
BseGI GGATG 3 cut(s) 199, 355, 454
BseJI GATNNNNATC 1 cut(s) 585
BseLI CCNNNNNNNGG 4 cut(s) 346, 393, 511, 626
BseNI ACTGG 2 cut(s) 340, 604
BseRI GAGGAG 2 cut(s) 532, 532
BseSI GKGCMC 1 cut(s) 427
BsgI GTGCAG 1 cut(s) 374
BshFI GGCC 2 cut(s) 5, 630
BshNI GGYRCC 1 cut(s) 536
BsiHKAI GWGCWC 3 cut(s) 411, 427, 456
BsiSI CCGG 1 cut(s) 102
BslFI GGGAC 1 cut(s) 610
BslI CCNNNNNNNGG 4 cut(s) 346, 393, 511, 626
BsmFI GGGAC 1 cut(s) 610
BsnI GGCC 2 cut(s) 5, 630
Bsp1286I GDGCHC 3 cut(s) 411, 427, 456
Bsp143I GATC 4 cut(s) 54, 70, 98, 551
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 2 cut(s) 144, 479
BspANI GGCC 2 cut(s) 5, 630
BspLI GGNNCC 1 cut(s) 538
BspOI GCTAGC 1 cut(s) 123
BspPI GGATC 4 cut(s) 62, 78, 93, 559
BspT107I GGYRCC 1 cut(s) 536
BspTI CTTAAG 1 cut(s) 509
BsrI ACTGG 2 cut(s) 340, 604
BssECI CCNNGG 3 cut(s) 6, 306, 474
BssMI GATC 4 cut(s) 54, 70, 98, 551
BssNI GRCGYC 1 cut(s) 105
BssT1I CCWWGG 1 cut(s) 6
Bst2UI CCWGG 2 cut(s) 388, 621
Bst4CI ACNGT 1 cut(s) 321
Bst6I CTCTTC 3 cut(s) 44, 60, 550
BstACI GRCGYC 1 cut(s) 105
BstAFI CTTAAG 1 cut(s) 509
BstC8I GCNNGC 2 cut(s) 121, 182
BstDEI CTNAG 1 cut(s) 377
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 3 cut(s) 199, 355, 454
BstKTI GATC 4 cut(s) 57, 73, 101, 554
BstMBI GATC 4 cut(s) 54, 70, 98, 551
BstNI CCWGG 2 cut(s) 388, 621
BstSCI CCNGG 2 cut(s) 386, 619
BstSLI GKGCMC 1 cut(s) 427
BstV2I GAAGAC 1 cut(s) 100
BstX2I RGATCY 3 cut(s) 70, 98, 551
BstYI RGATCY 3 cut(s) 70, 98, 551
Bsu36I CCTNAGG 1 cut(s) 377
BsuI GTATCC 1 cut(s) 481
BsuRI GGCC 2 cut(s) 5, 630
BtgI CCRYGG 1 cut(s) 6
BtsCI GGATG 3 cut(s) 199, 355, 454
Cac8I GCNNGC 2 cut(s) 121, 182
Cfr13I GGNCC 1 cut(s) 235
CseI GACGC 1 cut(s) 94
Csp6I GTAC 2 cut(s) 322, 522
CviAII CATG 3 cut(s) 7, 277, 490
CviQI GTAC 2 cut(s) 322, 522
DdeI CTNAG 1 cut(s) 377
DpnI GATC 4 cut(s) 56, 72, 100, 553
DpnII GATC 4 cut(s) 54, 70, 98, 551
DraIII CACNNNGTG 1 cut(s) 140
EaeI YGGCCR 2 cut(s) 3, 628
Eam1104I CTCTTC 3 cut(s) 44, 60, 550
EarI CTCTTC 3 cut(s) 44, 60, 550
EciI GGCGGA 1 cut(s) 494
Ecl136II GAGCTC 1 cut(s) 454
Eco130I CCWWGG 1 cut(s) 6
Eco24I GRGCYC 1 cut(s) 456
Eco47I GGWCC 1 cut(s) 235
Eco53kI GAGCTC 1 cut(s) 454
Eco81I CCTNAGG 1 cut(s) 377
EcoICRI GAGCTC 1 cut(s) 454
EcoRII CCWGG 2 cut(s) 386, 619
EcoT14I CCWWGG 1 cut(s) 6
EcoT22I ATGCAT 1 cut(s) 613
EcoT38I GRGCYC 1 cut(s) 456
ErhI CCWWGG 1 cut(s) 6
FaeI CATG 3 cut(s) 10, 280, 493
FaiI YATR 7 cut(s) 8, 139, 195, 278, 468, 491, 609
FalI AAGNNNNNCTT 2 cut(s) 57, 89
FaqI GGGAC 1 cut(s) 610
FatI CATG 3 cut(s) 6, 276, 489
FblI GTMKAC 1 cut(s) 210
FokI GGATG 3 cut(s) 186, 362, 461
FriOI GRGCYC 1 cut(s) 456
FspBI CTAG 2 cut(s) 24, 120
HaeIII GGCC 2 cut(s) 5, 630
HapII CCGG 1 cut(s) 102
HgaI GACGC 1 cut(s) 94
Hin1I GRCGYC 1 cut(s) 105
Hin1II CATG 3 cut(s) 10, 280, 493
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HinfI GANTC 2 cut(s) 283, 400
HpaII CCGG 1 cut(s) 102
HphI GGTGA 2 cut(s) 158, 162
Hpy166II GTNNAC 2 cut(s) 211, 425
Hpy188I TCNGA 1 cut(s) 307
Hpy188III TCNNGA 4 cut(s) 88, 233, 404, 549
Hpy8I GTNNAC 2 cut(s) 211, 425
HpyAV CCTTC 1 cut(s) 387
HpyCH4III ACNGT 1 cut(s) 321
HpyCH4V TGCA 4 cut(s) 355, 425, 584, 611
HpyF3I CTNAG 1 cut(s) 377
Hsp92I GRCGYC 1 cut(s) 105
Hsp92II CATG 3 cut(s) 10, 280, 493
Kzo9I GATC 4 cut(s) 54, 70, 98, 551
LmnI GCTCC 2 cut(s) 358, 378
LweI GCATC 1 cut(s) 571
MaeI CTAG 2 cut(s) 24, 120
MalI GATC 4 cut(s) 56, 72, 100, 553
MboI GATC 4 cut(s) 54, 70, 98, 551
MboII GAAGA 5 cut(s) 61, 65, 77, 100, 537
MflI RGATCY 3 cut(s) 70, 98, 551
MhlI GDGCHC 3 cut(s) 411, 427, 456
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 156, 300
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 2 cut(s) 525, 583
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 613
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 510, 634
Msp20I TGGCCA 1 cut(s) 5
MspCI CTTAAG 1 cut(s) 509
MspI CCGG 1 cut(s) 102
MspR9I CCNGG 2 cut(s) 388, 621
MvaI CCWGG 2 cut(s) 388, 621
NcoI CCATGG 1 cut(s) 6
NdeII GATC 4 cut(s) 54, 70, 98, 551
NheI GCTAGC 1 cut(s) 119
NlaIII CATG 3 cut(s) 10, 280, 493
NlaIV GGNNCC 1 cut(s) 538
NmeAIII GCCGAG 1 cut(s) 455
NsiI ATGCAT 1 cut(s) 613
PfeI GAWTC 2 cut(s) 283, 400
PflFI GACNNNGTC 1 cut(s) 215
Psp124BI GAGCTC 1 cut(s) 456
Psp6I CCWGG 2 cut(s) 386, 619
PspGI CCWGG 2 cut(s) 386, 619
PspN4I GGNNCC 1 cut(s) 538
PspPI GGNCC 1 cut(s) 235
PsuI RGATCY 3 cut(s) 70, 98, 551
PsyI GACNNNGTC 1 cut(s) 215
RsaI GTAC 2 cut(s) 323, 523
RsaNI GTAC 2 cut(s) 322, 522
SacI GAGCTC 1 cut(s) 456
SalI GTCGAC 1 cut(s) 209
SaqAI TTAA 2 cut(s) 510, 634
Sau3AI GATC 4 cut(s) 54, 70, 98, 551
Sau96I GGNCC 1 cut(s) 235
ScrFI CCNGG 2 cut(s) 388, 621
SduI GDGCHC 3 cut(s) 411, 427, 456
SetI ASST 7 cut(s) 31, 121, 153, 333, 414, 441, 456
SfaNI GCATC 1 cut(s) 571
SinI GGWCC 1 cut(s) 235
SmlI CTYRAG 1 cut(s) 509
SmoI CTYRAG 1 cut(s) 509
Sse9I AATT 2 cut(s) 156, 300
SsiI CCGC 2 cut(s) 144, 479
SspI AATATT 1 cut(s) 529
SspMI CTAG 2 cut(s) 24, 120
SstI GAGCTC 1 cut(s) 456
StyD4I CCNGG 2 cut(s) 386, 619
StyI CCWWGG 1 cut(s) 6
TaaI ACNGT 1 cut(s) 321
TaqI TCGA 6 cut(s) 57, 89, 210, 289, 315, 403
TasI AATT 2 cut(s) 156, 300
TatI WGTACW 1 cut(s) 321
TfiI GAWTC 2 cut(s) 283, 400
Tru1I TTAA 2 cut(s) 510, 634
Tru9I TTAA 2 cut(s) 510, 634
TspDTI ATGAA 2 cut(s) 62, 265
TspGWI ACGGA 1 cut(s) 109
Tth111I GACNNNGTC 1 cut(s) 215
Vha464I CTTAAG 1 cut(s) 509
VneI GTGCAC 1 cut(s) 423
VpaK11BI GGWCC 1 cut(s) 235
XapI RAATTY 1 cut(s) 300
XmiI GTMKAC 1 cut(s) 210
XspI CTAG 2 cut(s) 24, 120
Zsp2I ATGCAT 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.