pycom05g29630

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003577
Physical Location & Seq
Reverse (-)
30026780 .. 30030232
3453 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1440 bp
ATGAATATTGTATCAAAAGCGACGGCTTTCTTTGTTTTGGCTCTCGTTGCACTGCTGTTTGTGGAGCCTGTTTCGGGTAGAACGAAAAGGGCTAGTACTGAGAAACTGAACAACTCATTGGGGACTCAGGACCTTGTGACCAACTTGCCCGGCCAGCCTGCTGCAGACTTCAAGCACTACGCTGGCTATATGACCGTGAACGAAACAAATGGGAGGGCTCTTTTTTACTGGTTTTATGAGGCTGCCTCCAACCCAGATGGAAAACCTCTTGTGTTGTGGCTTAATGGAGGTCCTGGGTGCTCTTCTGTGGGATATGGAGCAACCCAAGAGATTGGTCCATTCATAGTGGACACTGATGGAAGTGGAATTAAATCTAATAACTACTCATGGAATAAAGAGGCCAATATGTTATTCTTGGAATCTCCAGTTGGGGTTGGCTTTTCATATTCTAATACAACTAGTGACTATGATAATCTCGGCGATGAGTTTACAGCGAATGATGCGTATAAATTCTTGCATAAGTGGTATCTCAAGTTCCCAACATACAGAACAAGGACCTTCTACATTTCTGGGGAGAGTTATGCAGGAAAGTATGTTCCAGAGCTCGCCGAGCTCATATATGACAAGAACAATGATCCTTCCCTTCATATTGATCTCAAGGGTATCCTGTTGGGTAATCCTGAAACATCTGATGCTGATGACTGGAGAGGTCTGGTAGATTATGCTTGGAGTCATGCTGTGATATCAGACGAAACTCACAAAATAATACGCGAAAGCTGTGATTTCGACAGAAATGATACTTGGAGTAACAAGGATTGTACTCTAGCTGTGGATGAAGTGCTAAAACAGTACAAGGAGATAGATATTTACAGCCTTTACACCTCGGTCTGCATTGGTGATACGGCAAGCTCAACTGATGGTTCGATGCAGGTCATGATGAAACGCACATCTACGATGATGCCGAGGATTATGGGTGGCTACGATCGATGTCTCGATGGATATTCAAAAACCTTTTATAACAGACCAGATGTGCAAAAGGCCCTCCATGTTAGTGATGGTGTCCGGCTCAAGAACTGGACCATCTGCAACAAAAAAATATTTGAGGACTGGGCATATTCGAAGCCTTCTGTTCTTCCTATATACAAGAAACTCATTGCAGCAGGACTTAGAATATGGGTGTACAGCGGAGACACAGATGGAAGAGTTCCTGTGCTTTCCACAAGATACAGCTTAAGTGCTCTAGGATTGCCTATTACTAAGGCATGGAGGCCTTGGTACCACGAGAAGCAGGTTAGTGGATGGTTTCAAGAATATGAAGGTCTTACATTTGCAACATTTAGAGGAGCTGGGCATGCAGTTCCCTGCTTCAAACCAAGCAACTCACTGGCTTTATTCACGTCTTTTCTCCTAGGCAATCCTCTCCCAACTGCTCGAGTATAA

Protein Analysis

480

Amino Acids

53.7

Weight (kDa)

6.11

Isoelectric Point (pI)

29.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 50 - 470 1.8e-141 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1017
Acc36I ACCTGC 2 cut(s) 919, 1279
Acc65I GGTACC 1 cut(s) 1275
AccB1I GGYRCC 1 cut(s) 1275
AccII CGCG 1 cut(s) 771
AciI CCGC 1 cut(s) 1185
AclWI GGATC 1 cut(s) 629
AcoI YGGCCR 1 cut(s) 151
AcsI RAATTY 1 cut(s) 509
AfaI GTAC 5 cut(s) 97, 820, 851, 1181, 1277
AfiI CCNNNNNNNGG 1 cut(s) 74
AflII CTTAAG 1 cut(s) 1231
AgsI TTSAA 4 cut(s) 172, 1005, 1307, 1369
AhlI ACTAGT 1 cut(s) 458
AjiI CACGTC 1 cut(s) 1398
AjnI CCWGG 1 cut(s) 292
AjuI GAANNNNNNNTTGG 8 cut(s) 101, 133, 395, 411, 427, 443, 784, 816
AloI GAACNNNNNNTCC 2 cut(s) 579, 611
AluBI AGCT 7 cut(s) 604, 613, 777, 827, 909, 1230, 1346
AluI AGCT 7 cut(s) 604, 613, 777, 827, 909, 1230, 1346
Alw21I GWGCWC 4 cut(s) 302, 606, 615, 1240
Alw26I GTCTC 2 cut(s) 995, 1182
AlwI GGATC 1 cut(s) 629
Ama87I CYCGRG 1 cut(s) 1431
AoxI GGCC 4 cut(s) 151, 399, 1038, 1268
ApeKI GCWGC 3 cut(s) 161, 242, 1157
ApoI RAATTY 1 cut(s) 509
Asp718I GGTACC 1 cut(s) 1275
AspA2I CCTAGG 1 cut(s) 1408
AspS9I GGNCC 6 cut(s) 130, 290, 335, 555, 1039, 1077
AsuC2I CCSGG 1 cut(s) 150
AsuHPI GGTGA 1 cut(s) 908
AsuII TTCGAA 1 cut(s) 1118
AvaI CYCGRG 1 cut(s) 1431
AvaII GGWCC 5 cut(s) 130, 290, 335, 555, 1077
AvrII CCTAGG 1 cut(s) 1408
BanI GGYRCC 1 cut(s) 1275
BanII GRGCYC 3 cut(s) 220, 606, 615
BauI CACGAG 1 cut(s) 1280
Bbv12I GWGCWC 4 cut(s) 302, 606, 615, 1240
BbvI GCAGC 3 cut(s) 148, 229, 1169
BccI CCATC 8 cut(s) 251, 350, 911, 989, 1049, 1088, 1190, 1293
BceAI ACGGC 2 cut(s) 39, 918
BciT130I CCWGG 1 cut(s) 294
BciVI GTATCC 1 cut(s) 674
BcnI CCSGG 1 cut(s) 150
BcoDI GTCTC 2 cut(s) 995, 1182
BcuI ACTAGT 1 cut(s) 458
BfaI CTAG 5 cut(s) 93, 459, 824, 1241, 1409
BfmI CTRYAG 1 cut(s) 162
BfrI CTTAAG 1 cut(s) 1231
BfuAI ACCTGC 2 cut(s) 919, 1279
BfuI GTATCC 1 cut(s) 674
BisI GCNGC 3 cut(s) 162, 243, 1158
BlnI CCTAGG 1 cut(s) 1408
BlsI GCNGC 3 cut(s) 163, 244, 1159
BmcAI AGTACT 1 cut(s) 97
Bme1390I CCNGG 2 cut(s) 150, 294
Bme18I GGWCC 5 cut(s) 130, 290, 335, 555, 1077
BmeT110I CYCGRG 1 cut(s) 1431
BmgBI CACGTC 1 cut(s) 1398
BmgT120I GGNCC 6 cut(s) 130, 290, 335, 555, 1039, 1077
BmiI GGNNCC 2 cut(s) 66, 1277
BmrFI CCNGG 2 cut(s) 150, 294
BmrI ACTGGG 1 cut(s) 1117
BmsI GCATC 4 cut(s) 490, 682, 915, 948
BmuI ACTGGG 1 cut(s) 1117
BplI GAGNNNNNCTC 2 cut(s) 230, 262
BpmI CTGGAG 2 cut(s) 408, 724
Bpu14I TTCGAA 1 cut(s) 1118
BpuEI CTTGAG 3 cut(s) 515, 641, 1052
BpuMI CCSGG 1 cut(s) 150
Bsa29I ATCGAT 1 cut(s) 985
BsaJI CCNNGG 5 cut(s) 293, 882, 962, 1271, 1408
Bsc4I CCNNNNNNNGG 1 cut(s) 74
Bse1I ACTGG 6 cut(s) 233, 425, 707, 1079, 1112, 1389
Bse3DI GCAATG 1 cut(s) 1152
BseBI CCWGG 1 cut(s) 294
BseCI ATCGAT 1 cut(s) 985
BseDI CCNNGG 5 cut(s) 293, 882, 962, 1271, 1408
BseGI GGATG 2 cut(s) 838, 1304
BseLI CCNNNNNNNGG 1 cut(s) 74
BseMI GCAATG 1 cut(s) 1152
BseMII CTCAG 2 cut(s) 90, 140
BseNI ACTGG 6 cut(s) 233, 425, 707, 1079, 1112, 1389
BseRI GAGGAG 1 cut(s) 1356
BseXI GCAGC 3 cut(s) 148, 229, 1169
BseYI CCCAGC 1 cut(s) 1346
Bsh1236I CGCG 1 cut(s) 771
Bsh1285I CGRYCG 1 cut(s) 985
BshFI GGCC 4 cut(s) 153, 401, 1040, 1270
BshNI GGYRCC 1 cut(s) 1275
BshVI ATCGAT 1 cut(s) 985
BsiEI CGRYCG 1 cut(s) 985
BsiHKAI GWGCWC 4 cut(s) 302, 606, 615, 1240
BsiHKCI CYCGRG 1 cut(s) 1431
BsiSI CCGG 2 cut(s) 150, 1063
BslFI GGGAC 1 cut(s) 136
BslI CCNNNNNNNGG 1 cut(s) 74
BsmAI GTCTC 2 cut(s) 995, 1182
BsmFI GGGAC 1 cut(s) 136
BsnI GGCC 4 cut(s) 153, 401, 1040, 1270
BsoBI CYCGRG 1 cut(s) 1431
Bsp119I TTCGAA 1 cut(s) 1118
Bsp1286I GDGCHC 5 cut(s) 220, 302, 606, 615, 1240
Bsp1407I TGTACA 1 cut(s) 1179
Bsp143I GATC 3 cut(s) 634, 652, 982
BspACI CCGC 1 cut(s) 1185
BspANI GGCC 4 cut(s) 153, 401, 1040, 1270
BspCNI CTCAG 2 cut(s) 91, 139
BspDI ATCGAT 1 cut(s) 985
BspFNI CGCG 1 cut(s) 771
BspHI TCATGA 1 cut(s) 933
BspLI GGNNCC 2 cut(s) 66, 1277
BspMAI CTGCAG 1 cut(s) 166
BspMI ACCTGC 2 cut(s) 919, 1279
BspPI GGATC 1 cut(s) 629
BspQI GCTCTTC 1 cut(s) 307
BspT104I TTCGAA 1 cut(s) 1118
BspT107I GGYRCC 1 cut(s) 1275
BspTI CTTAAG 1 cut(s) 1231
BsrDI GCAATG 1 cut(s) 1152
BsrGI TGTACA 1 cut(s) 1179
BsrI ACTGG 6 cut(s) 233, 425, 707, 1079, 1112, 1389
BssECI CCNNGG 5 cut(s) 293, 882, 962, 1271, 1408
BssMI GATC 3 cut(s) 634, 652, 982
BssSI CACGAG 1 cut(s) 1280
BssT1I CCWWGG 2 cut(s) 1271, 1408
Bst2BI CACGAG 1 cut(s) 1280
Bst2UI CCWGG 1 cut(s) 294
Bst4CI ACNGT 2 cut(s) 196, 849
Bst6I CTCTTC 2 cut(s) 307, 1195
BstAFI CTTAAG 1 cut(s) 1231
BstAUI TGTACA 1 cut(s) 1179
BstBI TTCGAA 1 cut(s) 1118
BstC8I GCNNGC 6 cut(s) 155, 159, 184, 606, 907, 1353
BstDEI CTNAG 4 cut(s) 99, 126, 1166, 1257
BstF5I GGATG 2 cut(s) 838, 1304
BstFNI CGCG 1 cut(s) 771
BstKTI GATC 3 cut(s) 637, 655, 985
BstMAI GTCTC 2 cut(s) 995, 1182
BstMBI GATC 3 cut(s) 634, 652, 982
BstMCI CGRYCG 1 cut(s) 985
BstMWI GCNNNNNNNGC 5 cut(s) 47, 154, 500, 610, 1352
BstNI CCWGG 1 cut(s) 294
BstNSI RCATGY 1 cut(s) 1355
BstSCI CCNGG 2 cut(s) 148, 292
BstSFI CTRYAG 1 cut(s) 162
BstUI CGCG 1 cut(s) 771
BstV1I GCAGC 3 cut(s) 148, 229, 1169
BstXI CCANNNNNNTGG 1 cut(s) 332
Bsu15I ATCGAT 1 cut(s) 985
BsuI GTATCC 1 cut(s) 674
BsuRI GGCC 4 cut(s) 153, 401, 1040, 1270
BsuTUI ATCGAT 1 cut(s) 985
BtgZI GCGATG 1 cut(s) 495
BtrI CACGTC 1 cut(s) 1398
BtsCI GGATG 2 cut(s) 838, 1304
BtsI GCAGTG 1 cut(s) 50
BtsIMutI CAGTG 3 cut(s) 50, 351, 1382
BveI ACCTGC 2 cut(s) 919, 1279
Cac8I GCNNGC 6 cut(s) 155, 159, 184, 606, 907, 1353
CciI TCATGA 1 cut(s) 933
Cfr13I GGNCC 6 cut(s) 130, 290, 335, 555, 1039, 1077
ClaI ATCGAT 1 cut(s) 985
Csp6I GTAC 5 cut(s) 96, 819, 850, 1180, 1276
CviAII CATG 6 cut(s) 387, 734, 934, 1046, 1263, 1352
CviQI GTAC 5 cut(s) 96, 819, 850, 1180, 1276
DdeI CTNAG 4 cut(s) 99, 126, 1166, 1257
DpnI GATC 3 cut(s) 636, 654, 984
DpnII GATC 3 cut(s) 634, 652, 982
EaeI YGGCCR 1 cut(s) 151
Eam1104I CTCTTC 2 cut(s) 307, 1195
EarI CTCTTC 2 cut(s) 307, 1195
Ecl136II GAGCTC 2 cut(s) 604, 613
Eco130I CCWWGG 2 cut(s) 1271, 1408
Eco147I AGGCCT 1 cut(s) 1270
Eco24I GRGCYC 3 cut(s) 220, 606, 615
Eco32I GATATC 1 cut(s) 744
Eco47I GGWCC 5 cut(s) 130, 290, 335, 555, 1077
Eco53kI GAGCTC 2 cut(s) 604, 613
Eco88I CYCGRG 1 cut(s) 1431
EcoICRI GAGCTC 2 cut(s) 604, 613
EcoO109I RGGNCCY 4 cut(s) 130, 290, 555, 1039
EcoRII CCWGG 1 cut(s) 292
EcoRV GATATC 1 cut(s) 744
EcoT14I CCWWGG 2 cut(s) 1271, 1408
EcoT38I GRGCYC 3 cut(s) 220, 606, 615
ErhI CCWWGG 2 cut(s) 1271, 1408
FaeI CATG 6 cut(s) 390, 737, 937, 1049, 1266, 1355
FaqI GGGAC 1 cut(s) 136
FatI CATG 6 cut(s) 386, 733, 933, 1045, 1262, 1351
Fnu4HI GCNGC 3 cut(s) 162, 243, 1158
FokI GGATG 2 cut(s) 845, 1311
FriOI GRGCYC 3 cut(s) 220, 606, 615
Fsp4HI GCNGC 3 cut(s) 162, 243, 1158
FspBI CTAG 5 cut(s) 93, 459, 824, 1241, 1409
GluI GCNGC 3 cut(s) 162, 243, 1158
GsaI CCCAGC 1 cut(s) 1350
GsuI CTGGAG 2 cut(s) 408, 724
HaeIII GGCC 4 cut(s) 153, 401, 1040, 1270
HapII CCGG 2 cut(s) 150, 1063
Hin1II CATG 6 cut(s) 390, 737, 937, 1049, 1266, 1355
HinfI GANTC 3 cut(s) 124, 419, 730
HpaII CCGG 2 cut(s) 150, 1063
HphI GGTGA 1 cut(s) 908
Hpy166II GTNNAC 4 cut(s) 199, 349, 489, 1180
Hpy188I TCNGA 2 cut(s) 691, 748
Hpy188III TCNNGA 7 cut(s) 128, 599, 680, 934, 992, 1069, 1307
Hpy8I GTNNAC 4 cut(s) 199, 349, 489, 1180
Hpy99I CGWCG 1 cut(s) 25
HpyAV CCTTC 5 cut(s) 568, 648, 653, 1134, 1310
HpyCH4III ACNGT 2 cut(s) 196, 849
HpyCH4IV ACGT 1 cut(s) 1397
HpyF10VI GCNNNNNNNGC 5 cut(s) 47, 154, 500, 610, 1352
HpyF3I CTNAG 4 cut(s) 99, 126, 1166, 1257
HpySE526I ACGT 1 cut(s) 1397
Hsp92II CATG 6 cut(s) 390, 737, 937, 1049, 1266, 1355
KpnI GGTACC 1 cut(s) 1279
Kzo9I GATC 3 cut(s) 634, 652, 982
LguI GCTCTTC 1 cut(s) 307
LmnI GCTCC 3 cut(s) 64, 317, 1343
Lsp1109I GCAGC 3 cut(s) 148, 229, 1169
LweI GCATC 4 cut(s) 490, 682, 915, 948
MaeI CTAG 5 cut(s) 93, 459, 824, 1241, 1409
MaeII ACGT 1 cut(s) 1397
MaeIII GTNAC 3 cut(s) 136, 461, 806
MalI GATC 3 cut(s) 636, 654, 984
MboI GATC 3 cut(s) 634, 652, 982
MboII GAAGA 3 cut(s) 294, 1124, 1212
MhlI GDGCHC 5 cut(s) 220, 302, 606, 615, 1240
MluCI AATT 2 cut(s) 366, 509
MlyI GAGTC 2 cut(s) 118, 739
MmeI TCCRAC 1 cut(s) 273
MseI TTAA 3 cut(s) 282, 369, 1232
MslI CAYNNNNRTG 1 cut(s) 1050
MspA1I CMGCKG 1 cut(s) 1185
MspCI CTTAAG 1 cut(s) 1231
MspI CCGG 2 cut(s) 150, 1063
MspR9I CCNGG 2 cut(s) 150, 294
MvaI CCWGG 1 cut(s) 294
MvnI CGCG 1 cut(s) 771
MwoI GCNNNNNNNGC 5 cut(s) 47, 154, 500, 610, 1352
NciI CCSGG 1 cut(s) 150
NdeII GATC 3 cut(s) 634, 652, 982
NlaIII CATG 6 cut(s) 390, 737, 937, 1049, 1266, 1355
NlaIV GGNNCC 2 cut(s) 66, 1277
NmeAIII GCCGAG 3 cut(s) 456, 634, 987
NmuCI GTSAC 2 cut(s) 136, 461
NspI RCATGY 1 cut(s) 1355
NspV TTCGAA 1 cut(s) 1118
PaeI GCATGC 1 cut(s) 1355
PaeR7I CTCGAG 1 cut(s) 1431
PagI TCATGA 1 cut(s) 933
PceI AGGCCT 1 cut(s) 1270
PciSI GCTCTTC 1 cut(s) 307
PcsI WCGNNNNNNNCGW 2 cut(s) 80, 959
PfeI GAWTC 1 cut(s) 419
PkrI GCNGC 3 cut(s) 163, 244, 1159
Ple19I CGATCG 1 cut(s) 985
PleI GAGTC 2 cut(s) 118, 738
PpsI GAGTC 2 cut(s) 118, 738
PpuMI RGGWCCY 3 cut(s) 130, 290, 555
PsiI TTATAA 1 cut(s) 1017
Psp124BI GAGCTC 2 cut(s) 606, 615
Psp5II RGGWCCY 3 cut(s) 130, 290, 555
Psp6I CCWGG 1 cut(s) 292
PspFI CCCAGC 1 cut(s) 1346
PspGI CCWGG 1 cut(s) 292
PspN4I GGNNCC 2 cut(s) 66, 1277
PspPI GGNCC 6 cut(s) 130, 290, 335, 555, 1039, 1077
PspPPI RGGWCCY 3 cut(s) 130, 290, 555
PspXI VCTCGAGB 1 cut(s) 1431
PsrI GAACNNNNNNTAC 2 cut(s) 518, 550
PstI CTGCAG 1 cut(s) 166
PvuI CGATCG 1 cut(s) 985
RsaI GTAC 5 cut(s) 97, 820, 851, 1181, 1277
RsaNI GTAC 5 cut(s) 96, 819, 850, 1180, 1276
RseI CAYNNNNRTG 1 cut(s) 1050
SacI GAGCTC 2 cut(s) 606, 615
SapI GCTCTTC 1 cut(s) 307
SaqAI TTAA 3 cut(s) 282, 369, 1232
SatI GCNGC 3 cut(s) 162, 243, 1158
Sau3AI GATC 3 cut(s) 634, 652, 982
Sau96I GGNCC 6 cut(s) 130, 290, 335, 555, 1039, 1077
ScaI AGTACT 1 cut(s) 97
SchI GAGTC 2 cut(s) 118, 739
ScrFI CCNGG 2 cut(s) 150, 294
SduI GDGCHC 5 cut(s) 220, 302, 606, 615, 1240
SfaNI GCATC 4 cut(s) 490, 682, 915, 948
SfcI CTRYAG 1 cut(s) 162
Sfr274I CTCGAG 1 cut(s) 1431
SfuI TTCGAA 1 cut(s) 1118
SinI GGWCC 5 cut(s) 130, 290, 335, 555, 1077
SlaI CTCGAG 1 cut(s) 1431
SmiMI CAYNNNNRTG 1 cut(s) 1050
SmlI CTYRAG 5 cut(s) 530, 656, 1067, 1231, 1431
SmoI CTYRAG 5 cut(s) 530, 656, 1067, 1231, 1431
SpeI ACTAGT 1 cut(s) 458
SphI GCATGC 1 cut(s) 1355
Sse9I AATT 2 cut(s) 366, 509
SseBI AGGCCT 1 cut(s) 1270
SsiI CCGC 1 cut(s) 1185
SspI AATATT 2 cut(s) 7, 1098
SspMI CTAG 5 cut(s) 93, 459, 824, 1241, 1409
SstI GAGCTC 2 cut(s) 606, 615
StuI AGGCCT 1 cut(s) 1270
StyD4I CCNGG 2 cut(s) 148, 292
StyI CCWWGG 2 cut(s) 1271, 1408
TaaI ACNGT 2 cut(s) 196, 849
TaiI ACGT 1 cut(s) 1400
TaqI TCGA 6 cut(s) 786, 923, 985, 993, 1118, 1432
TaqII GACCGA 1 cut(s) 874
TasI AATT 2 cut(s) 366, 509
TatI WGTACW 4 cut(s) 95, 818, 849, 1179
TfiI GAWTC 1 cut(s) 419
Tru1I TTAA 3 cut(s) 282, 369, 1232
Tru9I TTAA 3 cut(s) 282, 369, 1232
TscAI CASTG 3 cut(s) 57, 358, 1389
TseFI GTSAC 2 cut(s) 136, 461
TseI GCWGC 3 cut(s) 161, 242, 1157
Tsp45I GTSAC 2 cut(s) 136, 461
TspDTI ATGAA 7 cut(s) 17, 331, 432, 635, 849, 953, 1329
TspRI CASTG 3 cut(s) 57, 358, 1389
Vha464I CTTAAG 1 cut(s) 1231
VpaK11BI GGWCC 5 cut(s) 130, 290, 335, 555, 1077
XapI RAATTY 1 cut(s) 509
XceI RCATGY 1 cut(s) 1355
XcmI CCANNNNNNNNNTGG 1 cut(s) 1052
XhoI CTCGAG 1 cut(s) 1431
XmaJI CCTAGG 1 cut(s) 1408
XspI CTAG 5 cut(s) 93, 459, 824, 1241, 1409
ZrmI AGTACT 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.