pycom05g32370

Rho GDP-dissociation inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003575
Physical Location & Seq
Reverse (-)
31794393 .. 31796150
1758 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 675 bp
ATGTCTCTGGCCGTTGGAGTTGTTTCGAGTTCCAAAAACATGGGGCTGGATGATGAAAACACCGCCGAGGTCTCCGACAAGATGGCGAAAACTGGTGGGGCAAGTGCGGATCAACCGTCCACAGGGTTGGTTAGAGAACCCAGCGAGGGTTCCCTCTATCAGACCGAGGACGAGGACGACGATGACGAAGTAAAGAAGATTGAGTTGGGGCCTCAGTGCACTCTCAAAGAACAAATTGAGAAGGACGCGGTTCAAACTCTAGAACCAGATGTTAAGATCATGAGCCTTGCAATTAAATCCCCTGGCAGATCTGATATAATTCTTCCGATTCCTGAGAACGGAAATCCCAAAGGGTTGTGGTTTACTTTGAAAGAAGGTAGCCGTTACAGCCTCGAATTCACTATTCAGGTCAGCAATAACATTGTTTCAGGTCTCAAATACACTAACACAGTCTGGAAAACTGCTGTGAAAGTTGATAGCACAAAAGAGATGCTTGGAACATTTAGTCCACAGTCAGAGCCTTACACACATGTGTTGCCTGAGGACACAACCCCCTCGGGCATATTTGCTAGGGGATCTTATTCAGCTCGAAGCAAGTTTGTTGACGATGACAACAAGTGCTACTTGGAGATCAATTACACCTTTGATATCCGAAAAGACTGGCAATCGGCGTAA

Protein Analysis

225

Amino Acids

24.68

Weight (kDa)

4.58

Isoelectric Point (pI)

47.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_GDI PF02115 85 - 220 1.3e-61 RHO protein GDP dissociation inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 248
AciI CCGC 3 cut(s) 63, 107, 248
AclWI GGATC 2 cut(s) 117, 583
AcoI YGGCCR 1 cut(s) 9
AcsI RAATTY 1 cut(s) 395
AfiI CCNNNNNNNGG 3 cut(s) 122, 146, 338
AflIII ACRYGT 1 cut(s) 529
AgsI TTSAA 2 cut(s) 254, 370
AjnI CCWGG 1 cut(s) 301
AjuI GAANNNNNNNTTGG 2 cut(s) 188, 220
AleI CACNNNNGTG 1 cut(s) 530
AloI GAACNNNNNNTCC 2 cut(s) 490, 522
AluBI AGCT 1 cut(s) 587
AluI AGCT 1 cut(s) 587
Alw21I GWGCWC 1 cut(s) 221
Alw26I GTCTC 3 cut(s) 9, 76, 437
Alw44I GTGCAC 1 cut(s) 217
AlwI GGATC 2 cut(s) 117, 583
Ama87I CYCGRG 1 cut(s) 556
AoxI GGCC 2 cut(s) 9, 209
ApaLI GTGCAC 1 cut(s) 217
ApoI RAATTY 1 cut(s) 395
AspS9I GGNCC 1 cut(s) 209
AvaI CYCGRG 1 cut(s) 556
AxyI CCTNAGG 1 cut(s) 540
BaeGI GKGCMC 1 cut(s) 221
Bbv12I GWGCWC 1 cut(s) 221
BccI CCATC 1 cut(s) 76
BceAI ACGGC 1 cut(s) 366
BciT130I CCWGG 1 cut(s) 303
BcoDI GTCTC 3 cut(s) 9, 76, 437
BfaI CTAG 2 cut(s) 260, 570
BglII AGATCT 1 cut(s) 308
Bme1390I CCNGG 1 cut(s) 303
BmeT110I CYCGRG 1 cut(s) 556
BmgT120I GGNCC 1 cut(s) 209
BmiI GGNNCC 2 cut(s) 151, 210
BmrFI CCNGG 1 cut(s) 303
BmsI GCATC 1 cut(s) 480
BsaI GGTCTC 2 cut(s) 76, 437
BsaJI CCNNGG 4 cut(s) 66, 165, 301, 555
Bsc4I CCNNNNNNNGG 3 cut(s) 122, 146, 338
Bse1I ACTGG 2 cut(s) 97, 665
Bse21I CCTNAGG 1 cut(s) 540
BseBI CCWGG 1 cut(s) 303
BseDI CCNNGG 4 cut(s) 66, 165, 301, 555
BseGI GGATG 1 cut(s) 55
BseLI CCNNNNNNNGG 3 cut(s) 122, 146, 338
BseMII CTCAG 3 cut(s) 227, 324, 531
BseNI ACTGG 2 cut(s) 97, 665
BseSI GKGCMC 1 cut(s) 221
BseYI CCCAGC 1 cut(s) 140
Bsh1236I CGCG 1 cut(s) 248
BshFI GGCC 2 cut(s) 11, 211
BsiHKAI GWGCWC 1 cut(s) 221
BsiHKCI CYCGRG 1 cut(s) 556
BslI CCNNNNNNNGG 3 cut(s) 122, 146, 338
BsmAI GTCTC 3 cut(s) 9, 76, 437
BsnI GGCC 2 cut(s) 11, 211
Bso31I GGTCTC 2 cut(s) 76, 437
BsoBI CYCGRG 1 cut(s) 556
Bsp1286I GDGCHC 1 cut(s) 221
Bsp143I GATC 5 cut(s) 109, 276, 308, 575, 630
BspACI CCGC 3 cut(s) 63, 107, 248
BspANI GGCC 2 cut(s) 11, 211
BspCNI CTCAG 3 cut(s) 226, 325, 532
BspFNI CGCG 1 cut(s) 248
BspHI TCATGA 1 cut(s) 279
BspLI GGNNCC 2 cut(s) 151, 210
BspPI GGATC 2 cut(s) 117, 583
BspTNI GGTCTC 2 cut(s) 76, 437
BsrI ACTGG 2 cut(s) 97, 665
BssECI CCNNGG 4 cut(s) 66, 165, 301, 555
BssMI GATC 5 cut(s) 109, 276, 308, 575, 630
Bst2UI CCWGG 1 cut(s) 303
Bst4CI ACNGT 3 cut(s) 117, 451, 513
BstDEI CTNAG 3 cut(s) 213, 333, 540
BstF5I GGATG 1 cut(s) 55
BstFNI CGCG 1 cut(s) 248
BstKTI GATC 5 cut(s) 112, 279, 311, 578, 633
BstMAI GTCTC 3 cut(s) 9, 76, 437
BstMBI GATC 5 cut(s) 109, 276, 308, 575, 630
BstMWI GCNNNNNNNGC 1 cut(s) 387
BstNI CCWGG 1 cut(s) 303
BstNSI RCATGY 1 cut(s) 533
BstSCI CCNGG 1 cut(s) 301
BstSLI GKGCMC 1 cut(s) 221
BstUI CGCG 1 cut(s) 248
BstX2I RGATCY 2 cut(s) 308, 575
BstXI CCANNNNNNTGG 2 cut(s) 40, 127
BstYI RGATCY 2 cut(s) 308, 575
Bsu36I CCTNAGG 1 cut(s) 540
BsuRI GGCC 2 cut(s) 11, 211
BtsCI GGATG 1 cut(s) 55
BtsIMutI CAGTG 1 cut(s) 221
CciI TCATGA 1 cut(s) 279
Cfr13I GGNCC 1 cut(s) 209
CseI GACGC 1 cut(s) 254
CviAII CATG 3 cut(s) 40, 280, 530
CviJI RGCY 8 cut(s) 11, 46, 211, 285, 381, 390, 520, 587
CviKI_1 RGCY 8 cut(s) 11, 46, 211, 285, 381, 390, 520, 587
DdeI CTNAG 3 cut(s) 213, 333, 540
DpnI GATC 5 cut(s) 111, 278, 310, 577, 632
DpnII GATC 5 cut(s) 109, 276, 308, 575, 630
EaeI YGGCCR 1 cut(s) 9
Eco31I GGTCTC 2 cut(s) 76, 437
Eco32I GATATC 1 cut(s) 649
Eco81I CCTNAGG 1 cut(s) 540
Eco88I CYCGRG 1 cut(s) 556
EcoO109I RGGNCCY 1 cut(s) 209
EcoRI GAATTC 1 cut(s) 395
EcoRII CCWGG 1 cut(s) 301
EcoRV GATATC 1 cut(s) 649
FaeI CATG 3 cut(s) 43, 283, 533
FaiI YATR 5 cut(s) 41, 281, 317, 531, 563
FalI AAGNNNNNCTT 4 cut(s) 477, 509, 608, 640
FatI CATG 3 cut(s) 39, 279, 529
FokI GGATG 1 cut(s) 62
FspBI CTAG 2 cut(s) 260, 570
GsaI CCCAGC 1 cut(s) 144
HaeIII GGCC 2 cut(s) 11, 211
HgaI GACGC 1 cut(s) 254
Hin1II CATG 3 cut(s) 43, 283, 533
HincII GTYRAC 1 cut(s) 604
HindII GTYRAC 1 cut(s) 604
HinfI GANTC 1 cut(s) 328
Hpy166II GTNNAC 5 cut(s) 120, 219, 363, 509, 604
Hpy188I TCNGA 6 cut(s) 76, 162, 313, 327, 517, 653
Hpy188III TCNNGA 4 cut(s) 260, 280, 332, 454
Hpy8I GTNNAC 5 cut(s) 120, 219, 363, 509, 604
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 2 cut(s) 235, 368
HpyCH4III ACNGT 3 cut(s) 117, 451, 513
HpyCH4V TGCA 2 cut(s) 219, 290
HpyF10VI GCNNNNNNNGC 1 cut(s) 387
HpyF3I CTNAG 3 cut(s) 213, 333, 540
Hsp92II CATG 3 cut(s) 43, 283, 533
Kzo9I GATC 5 cut(s) 109, 276, 308, 575, 630
LweI GCATC 1 cut(s) 480
MaeI CTAG 2 cut(s) 260, 570
MaeIII GTNAC 1 cut(s) 383
MalI GATC 5 cut(s) 111, 278, 310, 577, 632
MboI GATC 5 cut(s) 109, 276, 308, 575, 630
MboII GAAGA 2 cut(s) 208, 314
MflI RGATCY 2 cut(s) 308, 575
MhlI GDGCHC 1 cut(s) 221
MluCI AATT 5 cut(s) 234, 291, 318, 395, 634
MmeI TCCRAC 1 cut(s) 99
MnlI CCTC 9 cut(s) 61, 139, 160, 164, 166, 222, 401, 535, 565
MseI TTAA 2 cut(s) 273, 294
MslI CAYNNNNRTG 1 cut(s) 530
MspR9I CCNGG 1 cut(s) 303
MvaI CCWGG 1 cut(s) 303
MvnI CGCG 1 cut(s) 248
MwoI GCNNNNNNNGC 1 cut(s) 387
NdeII GATC 5 cut(s) 109, 276, 308, 575, 630
NlaIII CATG 3 cut(s) 43, 283, 533
NlaIV GGNNCC 2 cut(s) 151, 210
NmeAIII GCCGAG 1 cut(s) 91
NspI RCATGY 1 cut(s) 533
OliI CACNNNNGTG 1 cut(s) 530
PagI TCATGA 1 cut(s) 279
PciI ACATGT 1 cut(s) 529
PcsI WCGNNNNNNNCGW 2 cut(s) 177, 183
PfeI GAWTC 1 cut(s) 328
PscI ACATGT 1 cut(s) 529
Psp6I CCWGG 1 cut(s) 301
PspFI CCCAGC 1 cut(s) 140
PspGI CCWGG 1 cut(s) 301
PspN4I GGNNCC 2 cut(s) 151, 210
PspPI GGNCC 1 cut(s) 209
PsuI RGATCY 2 cut(s) 308, 575
RseI CAYNNNNRTG 1 cut(s) 530
SaqAI TTAA 2 cut(s) 273, 294
Sau3AI GATC 5 cut(s) 109, 276, 308, 575, 630
Sau96I GGNCC 1 cut(s) 209
ScrFI CCNGG 1 cut(s) 303
SduI GDGCHC 1 cut(s) 221
SetI ASST 6 cut(s) 72, 379, 411, 433, 589, 644
SfaNI GCATC 1 cut(s) 480
SmiMI CAYNNNNRTG 1 cut(s) 530
Sse9I AATT 5 cut(s) 234, 291, 318, 395, 634
SsiI CCGC 3 cut(s) 63, 107, 248
SspMI CTAG 2 cut(s) 260, 570
StyD4I CCNGG 1 cut(s) 301
TaaI ACNGT 3 cut(s) 117, 451, 513
TaqI TCGA 3 cut(s) 26, 393, 589
TaqII GACCGA 1 cut(s) 179
TasI AATT 5 cut(s) 234, 291, 318, 395, 634
TfiI GAWTC 1 cut(s) 328
Tru1I TTAA 2 cut(s) 273, 294
Tru9I TTAA 2 cut(s) 273, 294
TscAI CASTG 1 cut(s) 221
TspDTI ATGAA 1 cut(s) 69
TspGWI ACGGA 1 cut(s) 354
TspRI CASTG 1 cut(s) 221
VneI GTGCAC 1 cut(s) 217
XapI RAATTY 1 cut(s) 395
XbaI TCTAGA 1 cut(s) 259
XceI RCATGY 1 cut(s) 533
XspI CTAG 2 cut(s) 260, 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.