pycom06g09420

ABC transporter C family member 10-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0002044
Physical Location & Seq
Reverse (-)
14736066 .. 14742233
6168 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4125 bp
ATGATGGGAAAACGGTTCGCATGGTTGCTTCTGGCTTTCACCACAAGCATAAACAAGCCACAACCGCACCATGTTGTGATAACAAAATCATGTTTAATACTTTCCTTCTTCGTTGCCATTTTTCTTTGCGGTTCATCAATTTGGGAAGCTATTGTTGATGAGGAAGTGTCAGTTAAGATTGTTTTAAACATTTTTTGTTTTCCTGGATCAATTCTTTTGCTATTTAGTGCATTTCAGGGAAACAATTTTTCCGAAGGTGGTTCAGAAATCCAGAATGATGCCTTTTACATGCCTCTGCAAGGTGCAGATATAATAGGTGAAATCAGTTCAGATGACAATGTAACACCTTTTGCAAAAGCAGGGTTATGGAGCTCAATATCATTTTGGTGGTTGAATCCTTTAATGACGAAAGGTAAGCAAAAAATTCTCGAGTATGAAGATATTCCACAGCTGCGTCAAGATGATCAAGCAAGAACGTGGTACTACATATTTATGGAGCAATTGAACAAACAGAAGGAAAGAGACTCATCTGTTAAACCTTCTTTCTTGTCCATAATTTTTCATTGCCAGAGGAAAGCTATTCTGACTTCGGGATTATTTGCACTGATCAAGACTCTCACCACATCAAGCAGTCCACTCTTTCTCATGGCTTTCATTAAGATTATTGAAGGCAATGCAGCTTTTAAATATGAAGGTTATGCCCTGACTTTTGGCCTCTTTGTTGTGAAAATCTTGGAGTCACTGTCGGCAAGGCAATGGTATTTTAAAACTAGACTGATCGGACTTGAAGTGAGATCGTTGGTGTCAGCAGCAATATATCACAAGCAACTTCGACTATCAAATGCTGCCAAGATGACTTATTCTCCTGGTGAGATAGTGAGTTATGTAACGGTGGATGCTTACAGAATCGGTGAGTTTCCATACTGGTTTCATCAGATATGGACCACAAGCCTTCAGCTGTGCCTTTCATTACTTATCGTTTACTTCTCCATGGGGCTGGCAATTGCTGCAGCTCTAGCTGTACTCATATTGAGTGCACTGGCAAGCTCGCCATTGGCGAAGTTGCAGCACGAGTATCAAACGAAGCTCGTGGTAGCACAAAACAGGAGGTTGAAGGCCATAACAGAAGCACTTTCAAATATGAAAGTACTGAAATTGTATTCCTGGGAGACAAATTTTAAGACTCTGATAGAAGGTTTACGAACCGAAGAGTTAAAATTGGTATATCGAGTGTTGTCACAAAAGGGATATAATATGGCTCTATTCTGGGCATCTCCTCTTTTCGTATCGGCAGTTACTTTTTGGACATGCTACTTCCTTGGATTTACACTCTCTCCTAGTAATGTTTTCATGTTTTTAGCAACTTTACGCCTTGTTCAGGAGCCTATTAGGTTGACTGCGGATGTCTTTGTAGCATTTATTGAAGCAAAGGTTTCATTTTCTCGGATTGAGAAGTTTCTCGATGCACAAGAGTTGGAGAATAGGCATACAAGGAAAGAGAGCTGTGCCACAGATGTTGAGCAGTCCATTTTATGTAGAAGTTCTGAAATTTCATGGGACACTAACCCTTCAAAGGCTACATTAAGGAGCATAAATTTGGTGGTGAAACCAGGAGAGAAGGTAGCAATTTGTGGAGAGGTTGGCTCCGGTAAATCCACCCTTTTAGCTGCAATTCTCGGAGAAGTTCCATGCATAAATGGCATTGTTCAAGTATATGGGAAGATAGCGTATGCTGCTCAGTCTGCGTGGATCCAAACTGGAACTATACAAGAAAATATTTTGTTTGGTTCTGCCATGGATAATGTTAGATACCGAGAAACACTTAGCAAGTGTTCCTTGGTGAAGGACCTTGAGATGCTTCCATTCCGCGATCTCACTCAGATTGGAGAAAGAGGTGTCAATCTGAGTGGTGGCCAGAAGCAGCGCATTCAACTTGTGCGTGCACTGTATCAAAATGCTGATGTCTATATCTTGGATGACCCTTTCAGCGCAGTTGATGCCCATACGGCGACAAACCAATTCAATGTAAGTGATAACTTGCCCCTCAAGGAATATGTCATGGGAGCTCTGTCAGGGAAGACGGTTTTACTTGTGACACATCAAGTAGACTTACTTCCAGCTTTTAATTCTATTTTGTTGATGTCTTCGGGGAAAATCTTGAGAGCAGCACCATACAAAGAGTTGCTGTCTTCTTGTGAAGAGTTCCAAGATCTTGTCCGTGCACACCATGACACTGCCGGTTGTGAGAGGCAAATGGAGAATGCTTCTAATAGACAATATAAATCATCAACGGTGGAGATTGAGGAAGTTAAACTGAAGTACAAGACAGAGACCGGGGACACTGGTTTCAAGCCATACATTCAGTACTTGAAACAACGCAAGGGATTTTTGTATTTCTCCTCATCAATCTTTTTTCATTTGATTTTCATAGTTGGGCAATTAATTCAATCCTATTGGTTGGCTTCCAAGCTACAGGATTATAGCGTATCAAGAGTCAAACTGTTTGTTGTGTATACAATGATCATGTTCATCATGTCATTTGGTGTGCTTCTTAGATTCTTCTGTTTAGTTGAATTAGGATGTGGAGCATCAAAATCCATTTTTGAAAAACTATTCAACTCTCTTTTCCGAGCACCAATGCTATTCTATGACTCAACACCAGTGGGAAGGATACTTAGTCGGGTCTCTAATGATATGAACATCGTCGACCTTGAAGTGGCTTTCAAGTTGGGAATTTCTGTTGGTTCAACTTTGATGACATACAGCATATATATAGTTTTGGTTTTGATTACTTGGCCAATCGTGTTTCTGATAATACCGATGATTTATGTCACTCTACTACTTCAGAAATACTACTTTGCTTCTGCAAAAGAATTAATGCGAATGAATGGCACGACTAAGTCTGCGCTTGCAAGCCACCTTGCCGAATCTATTGCTGGAGCCTTGACAATCAGAGCTTTTGGAGAGGAGGATCGGTTCTTCTCGAAAAACTTGGAATTTATTGATGCAAATGCAAGCGCTGACTTCAATAGGTTTTCAGCGAATGAGTGGTTGATTGAACGTCTTGAATTGCTGTGTGTAATTATTCTTTCAGCCTCAGCACTTGCAATCACTTTGATCCAATTTGACGCATCTTCCTCTGGTTTTATTGGGATGGCACTCTCGTTTGGTCTGTCCTTAAATGTATATGTTGTAGTTTCAGTTCAATTCCAAAGCATGCTAGAGAATTCAATGATATCTGTAGAAAGGGTAGAGCAATACATGCATATTCCCAGTGAAGCTCCAGAAGTAATCGAAGAAAACCGACCTGTGCACAGTTGGCCAACTGTTGGTAAAGTGGAAATACATGATTTAAAGGTTAGTGTACGCTATAGGCCAAATGCTCCGTTAGTACGCTATAGGCCAAATGCTCCGTTAGTTCTTCGTGGGATAAACTGTATAATTGAAGGAGGATACAAAATTGGGAATGTTGGCCGGACTGGAAGTGGGAAGACTACTCTTATCAGCGTTTTGTTTCGTCTGGTAGAGCCTACAGAGGGAAGGGTCATTGTCGATGACCATGACATTTGCAAAGTCGGGCTTCATGATTTAAGATCGCGTTTTGGGATCATACCACAAGATCCAACACTGTTCAGTGGATCTGTGAGATACAATCTTGACCCCTTATCAAAACATACTGATAATGAAATATGGGAGGTTTTGGAGAAATGTCAGTTACGAGAGGCCATCCAAGAAAAAGAGGAGGGCCTGGATTCATTAGGTAAGAAAACTGCATTTGTACAAGATGGAACAAACTGGAGCATGGGACAGCGTCAGCTATTCTGTTTGGGACGTGCTTTGTTGAAGAGGAGCCGAATACTAGTCCTCGATGAAGCCACTGCATCGATGGACAATGCAACAGACTCTATCTTACAGAAAACTATAAGAACAGGATTTGCGGATTGCACTGTAATAACAGTGGCTCACAGGATACCGACTGTGATGGACTGCACTAAAGTGCTTGCTATTAGTGATGAGTATGATGAGCCAATGAAGCTGATGAAGAATGAGGGCTCATTATTTGGGCAGCTAGTCAAGGAATATTGGTCTCGCACTGCCAATGCCGCCATACACTCAGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01523 ko01524 ko02010 ko04976 map01523 map01524 map02010 map04976
Pfam Domains
Protein Families
ABC_membrane ABC_tran

Protein Analysis

1375

Amino Acids

154.58

Weight (kDa)

7.87

Isoelectric Point (pI)

34.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABCC10_N PF24358 6 - 81 3.4e-21 ABCC10 N-terminal domain
ABC_membrane PF00664 195 - 462 6e-26 ABC transporter transmembrane region
ABC_tran PF00005 528 - 662 7.2e-21 ABC transporter
ABC_membrane PF00664 802 - 1055 1.1e-30 ABC transporter transmembrane region
ABC_tran PF00005 1141 - 1294 9.1e-23 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 3329
AccI GTMKAC 3 cut(s) 2106, 2513, 2706
AccII CGCG 2 cut(s) 1869, 3598
AciI CCGC 6 cut(s) 65, 129, 1400, 1867, 3938, 4104
AclWI GGATC 8 cut(s) 214, 1744, 1757, 2979, 3112, 3614, 3614, 3646
AcoI YGGCCR 4 cut(s) 1912, 2795, 3320, 3472
AcsI RAATTY 7 cut(s) 423, 1174, 1548, 1594, 2733, 2996, 3226
AcuI CTGAAG 3 cut(s) 938, 2336, 2828
AfaI GTAC 8 cut(s) 482, 1023, 1149, 2321, 2366, 3366, 3393, 3780
AfeI AGCGCT 1 cut(s) 3019
AfiI CCNNNNNNNGG 6 cut(s) 299, 1378, 1573, 2716, 3329, 3536
AhlI ACTAGT 1 cut(s) 3859
AjiI CACGTC 1 cut(s) 3833
AjnI CCWGG 5 cut(s) 202, 865, 1163, 1609, 3747
AleI CACNNNNGTG 1 cut(s) 3995
AloI GAACNNNNNNTCC 2 cut(s) 2960, 2992
Alw21I GWGCWC 7 cut(s) 374, 1039, 1945, 2068, 2224, 2635, 3315
Alw26I GTCTC 5 cut(s) 516, 1163, 2324, 2689, 4092
Alw44I GTGCAC 4 cut(s) 1035, 1941, 2220, 3311
AlwI GGATC 8 cut(s) 214, 1744, 1757, 2979, 3112, 3614, 3614, 3646
Ama87I CYCGRG 1 cut(s) 428
Aor51HI AGCGCT 1 cut(s) 3019
ApaLI GTGCAC 4 cut(s) 1035, 1941, 2220, 3311
ApoI RAATTY 7 cut(s) 423, 1174, 1548, 1594, 2733, 2996, 3226
AseI ATTAAT 2 cut(s) 2441, 2876
Asp700I GAANNNNTTC 1 cut(s) 441
AspLEI GCGC 4 cut(s) 1926, 1991, 2908, 3020
AspS9I GGNCC 3 cut(s) 942, 1846, 3745
AsuC2I CCSGG 1 cut(s) 2335
AsuHPI GGTGA 7 cut(s) 31, 329, 610, 881, 923, 1615, 1852
AvaI CYCGRG 1 cut(s) 428
AvaII GGWCC 2 cut(s) 942, 1846
BaeGI GKGCMC 4 cut(s) 1039, 1945, 2224, 3315
BalI TGGCCA 3 cut(s) 1914, 2797, 3322
BamHI GGATCC 1 cut(s) 1749
BanII GRGCYC 3 cut(s) 374, 2068, 4055
BarI GAAGNNNNNNTAC 2 cut(s) 2097, 2129
BauI CACGAG 2 cut(s) 1070, 1088
BbsI GAAGAC 4 cut(s) 2084, 2136, 2181, 3497
Bbv12I GWGCWC 7 cut(s) 374, 1039, 1945, 2068, 2224, 2635, 3315
BbvCI CCTCAGC 1 cut(s) 3097
BccI CCATC 5 cut(s) 3148, 3734, 3779, 3880, 3976
BceAI ACGGC 1 cut(s) 2022
BcgI CGANNNNNNTGC 2 cut(s) 2915, 2949
BciT130I CCWGG 5 cut(s) 204, 867, 1165, 1611, 3749
BciVI GTATCC 3 cut(s) 2664, 3446, 3963
BclI TGATCA 3 cut(s) 463, 606, 2520
BcnI CCSGG 1 cut(s) 2335
BcoDI GTCTC 5 cut(s) 516, 1163, 2324, 2689, 4092
BcuI ACTAGT 1 cut(s) 3859
BfaI CTAG 6 cut(s) 771, 1016, 1338, 3221, 3860, 4070
BfmI CTRYAG 6 cut(s) 1008, 2471, 3240, 3370, 3397, 3531
BfoI RGCGCY 1 cut(s) 3021
BfuI GTATCC 3 cut(s) 2664, 3446, 3963
BglI GCCNNNNNGGC 1 cut(s) 2006
BglII AGATCT 1 cut(s) 2209
BmcAI AGTACT 2 cut(s) 1149, 2366
Bme1390I CCNGG 6 cut(s) 204, 867, 1165, 1611, 2335, 3749
Bme18I GGWCC 2 cut(s) 942, 1846
BmeT110I CYCGRG 1 cut(s) 428
BmgBI CACGTC 1 cut(s) 3833
BmgT120I GGNCC 3 cut(s) 942, 1846, 3745
BmiI GGNNCC 5 cut(s) 1383, 1645, 1751, 2941, 3851
BmrFI CCNGG 6 cut(s) 204, 867, 1165, 1611, 2335, 3749
BmrI ACTGGG 1 cut(s) 3267
BmuI ACTGGG 1 cut(s) 3267
BpiI GAAGAC 4 cut(s) 2084, 2136, 2181, 3497
BplI GAGNNNNNCTC 2 cut(s) 1628, 1660
BpmI CTGGAG 3 cut(s) 2958, 3267, 3817
Bpu10I CCTNAGC 1 cut(s) 3097
BpuEI CTTGAG 3 cut(s) 1871, 2030, 2179
BpuMI CCSGG 1 cut(s) 2335
Bsa29I ATCGAT 1 cut(s) 3884
BsaI GGTCTC 3 cut(s) 2324, 2689, 4092
BsaJI CCNNGG 6 cut(s) 990, 1164, 1318, 1794, 1836, 2334
BsaWI WCCGGW 1 cut(s) 1646
Bsc4I CCNNNNNNNGG 6 cut(s) 299, 1378, 1573, 2716, 3329, 3536
Bse118I RCCGGY 1 cut(s) 2237
Bse1I ACTGG 8 cut(s) 929, 1044, 1762, 2347, 2660, 3273, 3484, 3800
Bse3DI GCAATG 3 cut(s) 562, 679, 761
BseBI CCWGG 5 cut(s) 204, 867, 1165, 1611, 3749
BseCI ATCGAT 1 cut(s) 3884
BseDI CCNNGG 6 cut(s) 990, 1164, 1318, 1794, 1836, 2334
BseGI GGATG 6 cut(s) 901, 1408, 1981, 2585, 3159, 3726
BseLI CCNNNNNNNGG 6 cut(s) 299, 1378, 1573, 2716, 3329, 3536
BseMI GCAATG 3 cut(s) 562, 679, 761
BseMII CTCAG 4 cut(s) 1751, 1892, 1895, 3111
BseNI ACTGG 8 cut(s) 929, 1044, 1762, 2347, 2660, 3273, 3484, 3800
BseRI GAGGAG 5 cut(s) 1266, 2389, 2981, 3755, 3862
BseSI GKGCMC 4 cut(s) 1039, 1945, 2224, 3315
BsgI GTGCAG 2 cut(s) 324, 3973
Bsh1236I CGCG 2 cut(s) 1869, 3598
BshVI ATCGAT 1 cut(s) 3884
BsiHKAI GWGCWC 7 cut(s) 374, 1039, 1945, 2068, 2224, 2635, 3315
BsiHKCI CYCGRG 1 cut(s) 428
BsiSI CCGG 4 cut(s) 1647, 2238, 2334, 3475
BslFI GGGAC 4 cut(s) 1571, 2351, 3819, 3843
BslI CCNNNNNNNGG 6 cut(s) 299, 1378, 1573, 2716, 3329, 3536
BsmAI GTCTC 5 cut(s) 516, 1163, 2324, 2689, 4092
BsmFI GGGAC 4 cut(s) 1571, 2351, 3819, 3843
BsmI GAATGC 2 cut(s) 1926, 2266
Bso31I GGTCTC 3 cut(s) 2324, 2689, 4092
BsoBI CYCGRG 1 cut(s) 428
Bsp1286I GDGCHC 8 cut(s) 374, 1039, 1945, 2068, 2224, 2635, 3315, 4055
Bsp1407I TGTACA 1 cut(s) 3778
Bsp19I CCATGG 2 cut(s) 990, 1794
BspACI CCGC 6 cut(s) 65, 129, 1400, 1867, 3938, 4104
BspCNI CTCAG 4 cut(s) 1750, 1891, 1896, 3110
BspDI ATCGAT 1 cut(s) 3884
BspFNI CGCG 2 cut(s) 1869, 3598
BspHI TCATGA 1 cut(s) 3583
BspLI GGNNCC 5 cut(s) 1383, 1645, 1751, 2941, 3851
BspMAI CTGCAG 1 cut(s) 1012
BspPI GGATC 8 cut(s) 214, 1744, 1757, 2979, 3112, 3614, 3614, 3646
BspTNI GGTCTC 3 cut(s) 2324, 2689, 4092
BsrDI GCAATG 3 cut(s) 562, 679, 761
BsrFI RCCGGY 1 cut(s) 2237
BsrGI TGTACA 1 cut(s) 3778
BsrI ACTGG 8 cut(s) 929, 1044, 1762, 2347, 2660, 3273, 3484, 3800
BssAI RCCGGY 1 cut(s) 2237
BssECI CCNNGG 6 cut(s) 990, 1164, 1318, 1794, 1836, 2334
BssNAI GTATAC 1 cut(s) 2514
BssSI CACGAG 2 cut(s) 1070, 1088
BssT1I CCWWGG 4 cut(s) 990, 1318, 1794, 1836
Bst1107I GTATAC 1 cut(s) 2514
Bst2BI CACGAG 2 cut(s) 1070, 1088
Bst2UI CCWGG 5 cut(s) 204, 867, 1165, 1611, 3749
Bst6I CTCTTC 3 cut(s) 1203, 2193, 3839
BstAPI GCANNNNNTGC 3 cut(s) 1007, 1997, 3262
BstAUI TGTACA 1 cut(s) 3778
BstC8I GCNNGC 9 cut(s) 999, 1045, 1049, 1941, 2910, 2914, 3016, 3218, 4002
BstDEI CTNAG 9 cut(s) 1737, 1823, 1878, 1904, 2552, 2675, 2898, 3097, 4114
BstDSI CCRYGG 2 cut(s) 990, 1794
BstENI CCTNNNNNAGG 2 cut(s) 297, 1376
BstF5I GGATG 6 cut(s) 901, 1408, 1981, 2585, 3159, 3726
BstFNI CGCG 2 cut(s) 1869, 3598
BstH2I RGCGCY 1 cut(s) 3021
BstHHI GCGC 4 cut(s) 1926, 1991, 2908, 3020
BstMAI GTCTC 5 cut(s) 516, 1163, 2324, 2689, 4092
BstNI CCWGG 5 cut(s) 204, 867, 1165, 1611, 3749
BstNSI RCATGY 4 cut(s) 292, 1311, 3220, 3265
BstSCI CCNGG 6 cut(s) 202, 865, 1163, 1609, 2333, 3747
BstSFI CTRYAG 6 cut(s) 1008, 2471, 3240, 3370, 3397, 3531
BstSLI GKGCMC 4 cut(s) 1039, 1945, 2224, 3315
BstUI CGCG 2 cut(s) 1869, 3598
BstV2I GAAGAC 4 cut(s) 2084, 2136, 2181, 3497
BstX2I RGATCY 4 cut(s) 1749, 2209, 3619, 3638
BstXI CCANNNNNNTGG 1 cut(s) 997
BstYI RGATCY 4 cut(s) 1749, 2209, 3619, 3638
BstZ17I GTATAC 1 cut(s) 2514
Bsu15I ATCGAT 1 cut(s) 3884
BsuI GTATCC 3 cut(s) 2664, 3446, 3963
BsuTUI ATCGAT 1 cut(s) 3884
BtgI CCRYGG 2 cut(s) 990, 1794
BtrI CACGTC 1 cut(s) 3833
BtsCI GGATG 6 cut(s) 901, 1408, 1981, 2585, 3159, 3726
BtsI GCAGTG 3 cut(s) 2232, 3876, 4092
Cac8I GCNNGC 9 cut(s) 999, 1045, 1049, 1941, 2910, 2914, 3016, 3218, 4002
CciI TCATGA 1 cut(s) 3583
CfoI GCGC 4 cut(s) 1926, 1991, 2908, 3020
Cfr10I RCCGGY 1 cut(s) 2237
Cfr13I GGNCC 3 cut(s) 942, 1846, 3745
ClaI ATCGAT 1 cut(s) 3884
CseI GACGC 3 cut(s) 443, 3137, 3800
Csp6I GTAC 8 cut(s) 481, 1022, 1148, 2320, 2365, 3365, 3392, 3779
CspCI CAANNNNNGTGG 6 cut(s) 1498, 1533, 1888, 1923, 2643, 2678
CviQI GTAC 8 cut(s) 481, 1022, 1148, 2320, 2365, 3365, 3392, 3779
DdeI CTNAG 9 cut(s) 1737, 1823, 1878, 1904, 2552, 2675, 2898, 3097, 4114
DraI TTTAAA 4 cut(s) 186, 685, 766, 3354
EaeI YGGCCR 4 cut(s) 1912, 2795, 3320, 3472
Eam1104I CTCTTC 3 cut(s) 1203, 2193, 3839
EarI CTCTTC 3 cut(s) 1203, 2193, 3839
Ecl136II GAGCTC 2 cut(s) 372, 2066
Eco130I CCWWGG 4 cut(s) 990, 1318, 1794, 1836
Eco24I GRGCYC 3 cut(s) 374, 2068, 4055
Eco31I GGTCTC 3 cut(s) 2324, 2689, 4092
Eco32I GATATC 1 cut(s) 3237
Eco47I GGWCC 2 cut(s) 942, 1846
Eco47III AGCGCT 1 cut(s) 3019
Eco53kI GAGCTC 2 cut(s) 372, 2066
Eco57I CTGAAG 3 cut(s) 938, 2336, 2828
Eco88I CYCGRG 1 cut(s) 428
EcoICRI GAGCTC 2 cut(s) 372, 2066
EcoNI CCTNNNNNAGG 2 cut(s) 297, 1376
EcoO109I RGGNCCY 2 cut(s) 1846, 3745
EcoRI GAATTC 1 cut(s) 3226
EcoRII CCWGG 5 cut(s) 202, 865, 1163, 1609, 3747
EcoRV GATATC 1 cut(s) 3237
EcoT14I CCWWGG 4 cut(s) 990, 1318, 1794, 1836
EcoT22I ATGCAT 2 cut(s) 1694, 3267
EcoT38I GRGCYC 3 cut(s) 374, 2068, 4055
ErhI CCWWGG 4 cut(s) 990, 1318, 1794, 1836
FalI AAGNNNNNCTT 4 cut(s) 1820, 1852, 3564, 3596
FaqI GGGAC 4 cut(s) 1571, 2351, 3819, 3843
FbaI TGATCA 3 cut(s) 463, 606, 2520
FblI GTMKAC 3 cut(s) 2106, 2513, 2706
FokI GGATG 6 cut(s) 908, 1415, 1988, 2592, 3166, 3713
FriOI GRGCYC 3 cut(s) 374, 2068, 4055
FspBI CTAG 6 cut(s) 771, 1016, 1338, 3221, 3860, 4070
GlaI GCGC 4 cut(s) 1925, 1990, 2907, 3019
GsuI CTGGAG 3 cut(s) 2958, 3267, 3817
HaeII RGCGCY 1 cut(s) 3021
HapII CCGG 4 cut(s) 1647, 2238, 2334, 3475
HgaI GACGC 3 cut(s) 443, 3137, 3800
HhaI GCGC 4 cut(s) 1926, 1991, 2908, 3020
Hin6I GCGC 4 cut(s) 1924, 1989, 2906, 3018
HinP1I GCGC 4 cut(s) 1924, 1989, 2906, 3018
HincII GTYRAC 2 cut(s) 1395, 2707
HindII GTYRAC 2 cut(s) 1395, 2707
HpaII CCGG 4 cut(s) 1647, 2238, 2334, 3475
HphI GGTGA 7 cut(s) 31, 329, 610, 881, 923, 1615, 1852
Hpy99I CGWCG 1 cut(s) 2708
HpyCH4IV ACGT 3 cut(s) 476, 3061, 3832
HpyF3I CTNAG 9 cut(s) 1737, 1823, 1878, 1904, 2552, 2675, 2898, 3097, 4114
HpySE526I ACGT 3 cut(s) 476, 3061, 3832
HspAI GCGC 4 cut(s) 1924, 1989, 2906, 3018
Ksp22I TGATCA 3 cut(s) 463, 606, 2520
MaeI CTAG 6 cut(s) 771, 1016, 1338, 3221, 3860, 4070
MaeII ACGT 3 cut(s) 476, 3061, 3832
MaeIII GTNAC 8 cut(s) 340, 738, 886, 1236, 1294, 2092, 2830, 3714
MfeI CAATTG 2 cut(s) 500, 1002
MflI RGATCY 4 cut(s) 1749, 2209, 3619, 3638
MhlI GDGCHC 8 cut(s) 374, 1039, 1945, 2068, 2224, 2635, 3315, 4055
MlsI TGGCCA 3 cut(s) 1914, 2797, 3322
MluNI TGGCCA 3 cut(s) 1914, 2797, 3322
MlyI GAGTC 7 cut(s) 518, 607, 746, 1177, 2502, 2645, 3896
MmeI TCCRAC 2 cut(s) 1455, 3647
Mox20I TGGCCA 3 cut(s) 1914, 2797, 3322
Mph1103I ATGCAT 2 cut(s) 1694, 3267
MroXI GAANNNNTTC 1 cut(s) 441
MscI TGGCCA 3 cut(s) 1914, 2797, 3322
MslI CAYNNNNRTG 4 cut(s) 385, 491, 3995, 4116
Msp20I TGGCCA 3 cut(s) 1914, 2797, 3322
MspA1I CMGCKG 2 cut(s) 451, 958
MspI CCGG 4 cut(s) 1647, 2238, 2334, 3475
MspR9I CCNGG 6 cut(s) 204, 867, 1165, 1611, 2335, 3749
MunI CAATTG 2 cut(s) 500, 1002
Mva1269I GAATGC 2 cut(s) 1926, 2266
MvaI CCWGG 5 cut(s) 204, 867, 1165, 1611, 3749
MvnI CGCG 2 cut(s) 1869, 3598
NciI CCSGG 1 cut(s) 2335
NcoI CCATGG 2 cut(s) 990, 1794
NlaIV GGNNCC 5 cut(s) 1383, 1645, 1751, 2941, 3851
NmuCI GTSAC 4 cut(s) 738, 1236, 2092, 2830
NsiI ATGCAT 2 cut(s) 1694, 3267
NspI RCATGY 4 cut(s) 292, 1311, 3220, 3265
OliI CACNNNNGTG 1 cut(s) 3995
PaeI GCATGC 1 cut(s) 3220
PaeR7I CTCGAG 1 cut(s) 428
PagI TCATGA 1 cut(s) 3583
PcsI WCGNNNNNNNCGW 1 cut(s) 1055
PctI GAATGC 2 cut(s) 1926, 2266
PdmI GAANNNNTTC 1 cut(s) 441
PfeI GAWTC 5 cut(s) 394, 906, 2556, 2927, 3752
PflFI GACNNNGTC 2 cut(s) 2899, 3810
PflMI CCANNNNNTGG 1 cut(s) 3329
PfoI TCCNGGA 1 cut(s) 202
PleI GAGTC 7 cut(s) 518, 607, 745, 1177, 2501, 2645, 3896
PpsI GAGTC 7 cut(s) 518, 607, 745, 1177, 2501, 2645, 3896
PpuMI RGGWCCY 1 cut(s) 1846
PshBI ATTAAT 2 cut(s) 2441, 2876
Psp124BI GAGCTC 2 cut(s) 374, 2068
Psp5II RGGWCCY 1 cut(s) 1846
Psp6I CCWGG 5 cut(s) 202, 865, 1163, 1609, 3747
PspGI CCWGG 5 cut(s) 202, 865, 1163, 1609, 3747
PspN4I GGNNCC 5 cut(s) 1383, 1645, 1751, 2941, 3851
PspPI GGNCC 3 cut(s) 942, 1846, 3745
PspPPI RGGWCCY 1 cut(s) 1846
PsrI GAACNNNNNNTAC 2 cut(s) 3186, 3218
PstI CTGCAG 1 cut(s) 1012
PsuI RGATCY 4 cut(s) 1749, 2209, 3619, 3638
PsyI GACNNNGTC 2 cut(s) 2899, 3810
PvuII CAGCTG 2 cut(s) 451, 958
RsaI GTAC 8 cut(s) 482, 1023, 1149, 2321, 2366, 3366, 3393, 3780
RsaNI GTAC 8 cut(s) 481, 1022, 1148, 2320, 2365, 3365, 3392, 3779
RseI CAYNNNNRTG 4 cut(s) 385, 491, 3995, 4116
SacI GAGCTC 2 cut(s) 374, 2068
SalI GTCGAC 1 cut(s) 2705
Sau96I GGNCC 3 cut(s) 942, 1846, 3745
ScaI AGTACT 2 cut(s) 1149, 2366
SchI GAGTC 7 cut(s) 518, 607, 746, 1177, 2502, 2645, 3896
ScrFI CCNGG 6 cut(s) 204, 867, 1165, 1611, 2335, 3749
SduI GDGCHC 8 cut(s) 374, 1039, 1945, 2068, 2224, 2635, 3315, 4055
SfcI CTRYAG 6 cut(s) 1008, 2471, 3240, 3370, 3397, 3531
Sfr274I CTCGAG 1 cut(s) 428
SinI GGWCC 2 cut(s) 942, 1846
SlaI CTCGAG 1 cut(s) 428
SmiMI CAYNNNNRTG 4 cut(s) 385, 491, 3995, 4116
SmlI CTYRAG 4 cut(s) 428, 1850, 2045, 2158
SmoI CTYRAG 4 cut(s) 428, 1850, 2045, 2158
SpeI ACTAGT 1 cut(s) 3859
SphI GCATGC 1 cut(s) 3220
SsiI CCGC 6 cut(s) 65, 129, 1400, 1867, 3938, 4104
SspI AATATT 2 cut(s) 1777, 4082
SspMI CTAG 6 cut(s) 771, 1016, 1338, 3221, 3860, 4070
SstI GAGCTC 2 cut(s) 374, 2068
StyD4I CCNGG 6 cut(s) 202, 865, 1163, 1609, 2333, 3747
StyI CCWWGG 4 cut(s) 990, 1318, 1794, 1836
TaiI ACGT 3 cut(s) 479, 3064, 3835
TatI WGTACW 5 cut(s) 1021, 1147, 2319, 2364, 3778
TauI GCSGC 1 cut(s) 4106
TfiI GAWTC 5 cut(s) 394, 906, 2556, 2927, 3752
TseFI GTSAC 4 cut(s) 738, 1236, 2092, 2830
Tsp45I GTSAC 4 cut(s) 738, 1236, 2092, 2830
TspGWI ACGGA 3 cut(s) 2207, 3375, 3402
Tth111I GACNNNGTC 2 cut(s) 2899, 3810
Van91I CCANNNNNTGG 1 cut(s) 3329
VneI GTGCAC 4 cut(s) 1035, 1941, 2220, 3311
VpaK11BI GGWCC 2 cut(s) 942, 1846
VspI ATTAAT 2 cut(s) 2441, 2876
XagI CCTNNNNNAGG 2 cut(s) 297, 1376
XapI RAATTY 7 cut(s) 423, 1174, 1548, 1594, 2733, 2996, 3226
XceI RCATGY 4 cut(s) 292, 1311, 3220, 3265
XcmI CCANNNNNNNNNTGG 1 cut(s) 3883
XhoI CTCGAG 1 cut(s) 428
XmiI GTMKAC 3 cut(s) 2106, 2513, 2706
XmnI GAANNNNTTC 1 cut(s) 441
XspI CTAG 6 cut(s) 771, 1016, 1338, 3221, 3860, 4070
ZrmI AGTACT 2 cut(s) 1149, 2366
Zsp2I ATGCAT 2 cut(s) 1694, 3267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.