pycom06g10220

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0003376
Physical Location & Seq
Forward (+)
15595435 .. 15597325
1891 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1065 bp
ATGGCCTCGGAGAGGGAAAGTTTCGACCTTTCAGGACCATTGCACCTAACTTCCATTGACTGGCAGAACACTCAGCATCGAAGATCTGTTGCTGCTTGTTTGGTTCAGGGTGTCTACATTCTTGAACGGGACCGCCAAGAGGAAAGAGAAGGGCCCCAGGCCCTTGCTCCTCCTTGGTGGGAGTTCTTCCATTTTAAGCTGCTCCGTAAGCTTGTGGATGATGTTGGTTTCTCTATCTTTGGTGCCTTATACGAGTTTAAACCTCCACCGTCTCTTTGTAACCGTCCATCGGAAGGAAGCCCTTGTTATGTGATTGCCTTCCGAGGCACCTTAACGAAGTATGACTCAGTCTCACGAGATCTTGAGTTGGATGTTGAGGTGATCCGAAATGGACTTCACAGGACATCTCGCTTCGAGATTGCTATGCAAGCTGTCCGAAATATGGTTGCTGCGTCAGGTCCTTCAAATGTTTGGTTAGCTGGCCATTCCTTGGGGTCAGCCATGGCAATGCTTGCTGGAAAAACTATGGCTAGCTCTGGCAATTATCTCGAATCTTTTCTCTTCAATCCGCCATTTGTGTCGGCCCCAATAGAGAGGATTCAGGATACAAGAGTGAAGCATGGGCTTCGGTTTGCAGGCAGTGTGATTACTGCTGGACTCACTCTTGCTATGAAGGCTAAACAGCAGCAACAACAACAACAACAACACAGAAGTCAACCAGAAGACGAACCATTCACTACTATGGCTGCATGGCTCCCAGGTCTATTTGTCAATCCAGCTGATGACATCTGCTCTGAGTATATAGGGTATTTCGAACATAGGAAAAAGATGGAGGACATTGGAGCAGGAGCCATTGAGCGGTTAGCAACCCAGAACTCTCTGGGGGGTCTACTAATGCACGCAATGGGAAAGCAGGCAGCACCCGAGCCGCCATTGCACCTCATTCCTTCTGCAAATCTGACTGTTAATTTAACCCCTTCAAGGGATCTGAAAGAAGCCCATGGAATTCATCAGTGGTGGAGAGACGACCTGCAATTGCTTTCCGAGGTCCACAGATACAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

355

Amino Acids

39.72

Weight (kDa)

6.5

Isoelectric Point (pI)

48.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1038
AccB1I GGYRCC 2 cut(s) 242, 326
AccB7I CCANNNNNTGG 2 cut(s) 60, 490
AccBSI CCGCTC 1 cut(s) 859
AccI GTMKAC 2 cut(s) 114, 889
AciI CCGC 4 cut(s) 133, 569, 859, 929
AclWI GGATC 2 cut(s) 376, 993
AcoI YGGCCR 1 cut(s) 481
AcsI RAATTY 1 cut(s) 1005
AgsI TTSAA 4 cut(s) 125, 465, 565, 981
AjnI CCWGG 2 cut(s) 156, 757
AluBI AGCT 6 cut(s) 199, 211, 431, 479, 534, 779
AluI AGCT 6 cut(s) 199, 211, 431, 479, 534, 779
Alw26I GTCTC 3 cut(s) 276, 355, 1017
AlwI GGATC 2 cut(s) 376, 993
Ama87I CYCGRG 1 cut(s) 923
AoxI GGCC 5 cut(s) 3, 152, 159, 481, 582
ApaI GGGCCC 1 cut(s) 156
ApeKI GCWGC 6 cut(s) 92, 199, 449, 685, 746, 917
ApoI RAATTY 1 cut(s) 1005
Asp700I GAANNNNTTC 1 cut(s) 555
AspS9I GGNCC 8 cut(s) 35, 130, 152, 153, 160, 458, 583, 1048
AsuHPI GGTGA 1 cut(s) 391
AsuII TTCGAA 1 cut(s) 813
AsuNHI GCTAGC 1 cut(s) 530
AvaI CYCGRG 1 cut(s) 923
AvaII GGWCC 4 cut(s) 35, 130, 458, 1048
BaeGI GKGCMC 1 cut(s) 156
BaeI ACNNNNGTAYC 2 cut(s) 597, 630
BalI TGGCCA 1 cut(s) 483
BanI GGYRCC 2 cut(s) 242, 326
BanII GRGCYC 1 cut(s) 156
BauI CACGAG 1 cut(s) 354
BbsI GAAGAC 1 cut(s) 729
BbvI GCAGC 6 cut(s) 79, 186, 436, 697, 733, 929
BccI CCATC 2 cut(s) 295, 823
BcgI CGANNNNNNTGC 4 cut(s) 529, 563, 608, 642
BciT130I CCWGG 2 cut(s) 158, 759
BciVI GTATCC 1 cut(s) 598
BcoDI GTCTC 3 cut(s) 276, 355, 1017
BfaI CTAG 1 cut(s) 531
BfuAI ACCTGC 1 cut(s) 1038
BfuI GTATCC 1 cut(s) 598
BglII AGATCT 2 cut(s) 83, 358
BisI GCNGC 7 cut(s) 93, 200, 450, 686, 747, 918, 929
BlsI GCNGC 7 cut(s) 94, 201, 451, 687, 748, 919, 930
Bme1390I CCNGG 2 cut(s) 158, 759
Bme18I GGWCC 4 cut(s) 35, 130, 458, 1048
BmeT110I CYCGRG 1 cut(s) 923
BmgT120I GGNCC 8 cut(s) 35, 130, 152, 153, 160, 458, 583, 1048
BmiI GGNNCC 8 cut(s) 131, 154, 155, 244, 328, 585, 755, 850
BmrFI CCNGG 2 cut(s) 158, 759
BmsI GCATC 1 cut(s) 85
BmtI GCTAGC 1 cut(s) 534
BpiI GAAGAC 1 cut(s) 729
Bpu14I TTCGAA 1 cut(s) 813
BpuEI CTTGAG 1 cut(s) 383
BsaJI CCNNGG 9 cut(s) 6, 156, 173, 322, 489, 501, 757, 1000, 1044
Bse1I ACTGG 1 cut(s) 65
Bse3DI GCAATG 4 cut(s) 38, 513, 909, 932
BseBI CCWGG 2 cut(s) 158, 759
BseDI CCNNGG 9 cut(s) 6, 156, 173, 322, 489, 501, 757, 1000, 1044
BseGI GGATG 2 cut(s) 223, 376
BseMI GCAATG 4 cut(s) 38, 513, 909, 932
BseMII CTCAG 3 cut(s) 86, 360, 786
BseNI ACTGG 1 cut(s) 65
BseRI GAGGAG 1 cut(s) 159
BseSI GKGCMC 1 cut(s) 156
BseXI GCAGC 6 cut(s) 79, 186, 436, 697, 733, 929
BshFI GGCC 5 cut(s) 5, 154, 161, 483, 584
BshNI GGYRCC 2 cut(s) 242, 326
BsiHKCI CYCGRG 1 cut(s) 923
BslFI GGGAC 1 cut(s) 143
BsmAI GTCTC 3 cut(s) 276, 355, 1017
BsmBI CGTCTC 2 cut(s) 276, 1017
BsmFI GGGAC 1 cut(s) 143
BsnI GGCC 5 cut(s) 5, 154, 161, 483, 584
BsoBI CYCGRG 1 cut(s) 923
Bsp119I TTCGAA 1 cut(s) 813
Bsp120I GGGCCC 1 cut(s) 152
Bsp1286I GDGCHC 1 cut(s) 156
Bsp143I GATC 4 cut(s) 83, 358, 381, 985
Bsp19I CCATGG 2 cut(s) 501, 1000
BspACI CCGC 4 cut(s) 133, 569, 859, 929
BspANI GGCC 5 cut(s) 5, 154, 161, 483, 584
BspCNI CTCAG 3 cut(s) 85, 359, 787
BspLI GGNNCC 8 cut(s) 131, 154, 155, 244, 328, 585, 755, 850
BspMI ACCTGC 1 cut(s) 1038
BspOI GCTAGC 1 cut(s) 534
BspPI GGATC 2 cut(s) 376, 993
BspT104I TTCGAA 1 cut(s) 813
BspT107I GGYRCC 2 cut(s) 242, 326
BsrBI CCGCTC 1 cut(s) 859
BsrDI GCAATG 4 cut(s) 38, 513, 909, 932
BsrI ACTGG 1 cut(s) 65
BssECI CCNNGG 9 cut(s) 6, 156, 173, 322, 489, 501, 757, 1000, 1044
BssMI GATC 4 cut(s) 83, 358, 381, 985
BssSI CACGAG 1 cut(s) 354
BssT1I CCWWGG 4 cut(s) 173, 489, 501, 1000
Bst2BI CACGAG 1 cut(s) 354
Bst2UI CCWGG 2 cut(s) 158, 759
Bst4CI ACNGT 3 cut(s) 270, 284, 964
Bst6I CTCTTC 1 cut(s) 566
BstAPI GCANNNNNTGC 1 cut(s) 512
BstBI TTCGAA 1 cut(s) 813
BstC8I GCNNGC 7 cut(s) 429, 481, 513, 532, 637, 900, 915
BstDEI CTNAG 3 cut(s) 72, 346, 795
BstDSI CCRYGG 2 cut(s) 501, 1000
BstENI CCTNNNNNAGG 1 cut(s) 10
BstF5I GGATG 2 cut(s) 223, 376
BstKTI GATC 4 cut(s) 86, 361, 384, 988
BstMAI GTCTC 3 cut(s) 276, 355, 1017
BstMBI GATC 4 cut(s) 83, 358, 381, 985
BstMWI GCNNNNNNNGC 5 cut(s) 208, 428, 512, 674, 934
BstNI CCWGG 2 cut(s) 158, 759
BstSCI CCNGG 2 cut(s) 156, 757
BstSLI GKGCMC 1 cut(s) 156
BstV1I GCAGC 6 cut(s) 79, 186, 436, 697, 733, 929
BstV2I GAAGAC 1 cut(s) 729
BstX2I RGATCY 3 cut(s) 83, 358, 985
BstYI RGATCY 3 cut(s) 83, 358, 985
BsuI GTATCC 1 cut(s) 598
BsuRI GGCC 5 cut(s) 5, 154, 161, 483, 584
BtgI CCRYGG 2 cut(s) 501, 1000
BtsCI GGATG 2 cut(s) 223, 376
BtsI GCAGTG 1 cut(s) 646
BtsIMutI CAGTG 2 cut(s) 646, 1019
BveI ACCTGC 1 cut(s) 1038
Cac8I GCNNGC 7 cut(s) 429, 481, 513, 532, 637, 900, 915
Cfr13I GGNCC 8 cut(s) 35, 130, 152, 153, 160, 458, 583, 1048
CseI GACGC 1 cut(s) 441
CviAII CATG 4 cut(s) 502, 620, 750, 1001
DdeI CTNAG 3 cut(s) 72, 346, 795
DpnI GATC 4 cut(s) 85, 360, 383, 987
DpnII GATC 4 cut(s) 83, 358, 381, 985
DraI TTTAAA 1 cut(s) 259
EaeI YGGCCR 1 cut(s) 481
Eam1104I CTCTTC 1 cut(s) 566
EarI CTCTTC 1 cut(s) 566
EciI GGCGGA 1 cut(s) 558
Eco130I CCWWGG 4 cut(s) 173, 489, 501, 1000
Eco24I GRGCYC 1 cut(s) 156
Eco47I GGWCC 4 cut(s) 35, 130, 458, 1048
Eco88I CYCGRG 1 cut(s) 923
EcoNI CCTNNNNNAGG 1 cut(s) 10
EcoO109I RGGNCCY 4 cut(s) 152, 153, 160, 458
EcoRI GAATTC 1 cut(s) 1005
EcoRII CCWGG 2 cut(s) 156, 757
EcoT14I CCWWGG 4 cut(s) 173, 489, 501, 1000
EcoT38I GRGCYC 1 cut(s) 156
ErhI CCWWGG 4 cut(s) 173, 489, 501, 1000
Esp3I CGTCTC 2 cut(s) 276, 1017
FaeI CATG 4 cut(s) 505, 623, 753, 1004
FaqI GGGAC 1 cut(s) 143
FatI CATG 4 cut(s) 501, 619, 749, 1000
FblI GTMKAC 2 cut(s) 114, 889
Fnu4HI GCNGC 7 cut(s) 93, 200, 450, 686, 747, 918, 929
FokI GGATG 2 cut(s) 230, 383
FriOI GRGCYC 1 cut(s) 156
Fsp4HI GCNGC 7 cut(s) 93, 200, 450, 686, 747, 918, 929
FspBI CTAG 1 cut(s) 531
GluI GCNGC 7 cut(s) 93, 200, 450, 686, 747, 918, 929
HaeIII GGCC 5 cut(s) 5, 154, 161, 483, 584
HgaI GACGC 1 cut(s) 441
Hin1II CATG 4 cut(s) 505, 623, 753, 1004
HincII GTYRAC 1 cut(s) 716
HindII GTYRAC 1 cut(s) 716
HindIII AAGCTT 1 cut(s) 209
HinfI GANTC 4 cut(s) 344, 551, 598, 657
HphI GGTGA 1 cut(s) 391
Hpy166II GTNNAC 4 cut(s) 115, 716, 890, 1051
Hpy188I TCNGA 9 cut(s) 10, 292, 323, 386, 437, 796, 960, 990, 1045
Hpy188III TCNNGA 7 cut(s) 33, 122, 354, 362, 415, 548, 602
Hpy8I GTNNAC 4 cut(s) 115, 716, 890, 1051
HpyAV CCTTC 7 cut(s) 143, 287, 328, 471, 667, 957, 987
HpyCH4III ACNGT 3 cut(s) 270, 284, 964
HpyCH4V TGCA 8 cut(s) 43, 427, 635, 749, 898, 937, 953, 1033
HpyF10VI GCNNNNNNNGC 5 cut(s) 208, 428, 512, 674, 934
HpyF3I CTNAG 3 cut(s) 72, 346, 795
Hsp92II CATG 4 cut(s) 505, 623, 753, 1004
Kzo9I GATC 4 cut(s) 83, 358, 381, 985
LmnI GCTCC 5 cut(s) 172, 207, 759, 842, 848
Lsp1109I GCAGC 6 cut(s) 79, 186, 436, 697, 733, 929
LweI GCATC 1 cut(s) 85
MaeI CTAG 1 cut(s) 531
MaeIII GTNAC 1 cut(s) 278
MalI GATC 4 cut(s) 85, 360, 383, 987
MbiI CCGCTC 1 cut(s) 859
MboI GATC 4 cut(s) 83, 358, 381, 985
MboII GAAGA 4 cut(s) 93, 178, 553, 734
MfeI CAATTG 1 cut(s) 1034
MflI RGATCY 3 cut(s) 83, 358, 985
MhlI GDGCHC 1 cut(s) 156
MlsI TGGCCA 1 cut(s) 483
MluCI AATT 4 cut(s) 541, 967, 1005, 1034
MluNI TGGCCA 1 cut(s) 483
MlyI GAGTC 2 cut(s) 338, 651
MmeI TCCRAC 1 cut(s) 348
Mox20I TGGCCA 1 cut(s) 483
MroXI GAANNNNTTC 1 cut(s) 555
MscI TGGCCA 1 cut(s) 483
MseI TTAA 5 cut(s) 195, 258, 332, 966, 971
MslI CAYNNNNRTG 2 cut(s) 506, 740
Msp20I TGGCCA 1 cut(s) 483
MspA1I CMGCKG 1 cut(s) 779
MspR9I CCNGG 2 cut(s) 158, 759
MssI GTTTAAAC 1 cut(s) 259
MunI CAATTG 1 cut(s) 1034
MvaI CCWGG 2 cut(s) 158, 759
MwoI GCNNNNNNNGC 5 cut(s) 208, 428, 512, 674, 934
NcoI CCATGG 2 cut(s) 501, 1000
NdeII GATC 4 cut(s) 83, 358, 381, 985
NheI GCTAGC 1 cut(s) 530
NlaIII CATG 4 cut(s) 505, 623, 753, 1004
NlaIV GGNNCC 8 cut(s) 131, 154, 155, 244, 328, 585, 755, 850
NspV TTCGAA 1 cut(s) 813
PdmI GAANNNNTTC 1 cut(s) 555
PfeI GAWTC 2 cut(s) 551, 598
PflFI GACNNNGTC 1 cut(s) 347
PflMI CCANNNNNTGG 2 cut(s) 60, 490
PkrI GCNGC 7 cut(s) 94, 201, 451, 687, 748, 919, 930
PleI GAGTC 2 cut(s) 338, 651
PmeI GTTTAAAC 1 cut(s) 259
PpsI GAGTC 2 cut(s) 338, 651
PpuMI RGGWCCY 1 cut(s) 458
Psp5II RGGWCCY 1 cut(s) 458
Psp6I CCWGG 2 cut(s) 156, 757
PspGI CCWGG 2 cut(s) 156, 757
PspN4I GGNNCC 8 cut(s) 131, 154, 155, 244, 328, 585, 755, 850
PspOMI GGGCCC 1 cut(s) 152
PspPI GGNCC 8 cut(s) 35, 130, 152, 153, 160, 458, 583, 1048
PspPPI RGGWCCY 1 cut(s) 458
PsuI RGATCY 3 cut(s) 83, 358, 985
PsyI GACNNNGTC 1 cut(s) 347
PvuII CAGCTG 1 cut(s) 779
RseI CAYNNNNRTG 2 cut(s) 506, 740
SaqAI TTAA 5 cut(s) 195, 258, 332, 966, 971
SatI GCNGC 7 cut(s) 93, 200, 450, 686, 747, 918, 929
Sau3AI GATC 4 cut(s) 83, 358, 381, 985
Sau96I GGNCC 8 cut(s) 35, 130, 152, 153, 160, 458, 583, 1048
SchI GAGTC 2 cut(s) 338, 651
ScrFI CCNGG 2 cut(s) 158, 759
SduI GDGCHC 1 cut(s) 156
SfaNI GCATC 1 cut(s) 85
SfuI TTCGAA 1 cut(s) 813
SinI GGWCC 4 cut(s) 35, 130, 458, 1048
SmiMI CAYNNNNRTG 2 cut(s) 506, 740
SmlI CTYRAG 1 cut(s) 362
SmoI CTYRAG 1 cut(s) 362
Sse9I AATT 4 cut(s) 541, 967, 1005, 1034
SsiI CCGC 4 cut(s) 133, 569, 859, 929
SspMI CTAG 1 cut(s) 531
StyD4I CCNGG 2 cut(s) 156, 757
StyI CCWWGG 4 cut(s) 173, 489, 501, 1000
TaaI ACNGT 3 cut(s) 270, 284, 964
TaqI TCGA 5 cut(s) 24, 79, 414, 549, 813
TasI AATT 4 cut(s) 541, 967, 1005, 1034
TauI GCSGC 1 cut(s) 931
TfiI GAWTC 2 cut(s) 551, 598
Tru1I TTAA 5 cut(s) 195, 258, 332, 966, 971
Tru9I TTAA 5 cut(s) 195, 258, 332, 966, 971
TscAI CASTG 2 cut(s) 646, 1019
TseI GCWGC 6 cut(s) 92, 199, 449, 685, 746, 917
TspDTI ATGAA 2 cut(s) 686, 998
TspGWI ACGGA 1 cut(s) 194
TspRI CASTG 2 cut(s) 646, 1019
Tth111I GACNNNGTC 1 cut(s) 347
Van91I CCANNNNNTGG 2 cut(s) 60, 490
VpaK11BI GGWCC 4 cut(s) 35, 130, 458, 1048
XagI CCTNNNNNAGG 1 cut(s) 10
XapI RAATTY 1 cut(s) 1005
XmiI GTMKAC 2 cut(s) 114, 889
XmnI GAANNNNTTC 1 cut(s) 555
XspI CTAG 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.