pycom07g01640

Uncharacterized protein family, UPF0114

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0005158
Physical Location & Seq
Forward (+)
1294506 .. 1296941
2436 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1038 bp
ATGTCGTTCATTAGCCCAAAAGTCCCACGCTCCCCTTCCACGCGCGAAGGCCACTTTCGCTCCCAAATTCCGTTACTACCCGCCATCCAAAGGCGCAAAAAAATTCTCAGCCCTCCAACCAAAATATCTCCATCCGGCAAACTCCTCCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCCCTACCTCTACACAATGGCTCTCTTCACTTCGATCTCCTCCTCAACCCTCTGCCTCTCCGCCTCAATCCATCCCATCGGGCCGTCGAACACCTCTAAACGGTTCTCTCGTCCCATCGCTGCTTCTTCTCTGTGCTCCCCACCGTCTCCGGAATCAAAATCAACGGCCCCAAAACAAACCGTAGTCTCCTCCTCCACTGAAACTTCACCAAACCATTTTAATGGTGCGTCCTCTAGACCCCCCGAGCCGGCGGGCTTCAACTACGCCCTTGCCAACGGCAACCCCTTTGTCCGGTTTGCCCGTGCCGCTGAGTCTTCCATTGAAAGGACAATATTTGACTTCCGGTTTTTGGCACTGTTTGCTGTTGGAGGTTCGTTGGCTGGTTCGTTGTTGTGCTTTCTCAATGGTTGTGTTTATATAATGGATGCGTATAAAGTTTACTGGACTAGTTGTGTCAAAGGGTATCACACTGGACATATGGTTCTACGACTTGTTGAAGCTATCGATGTTTACCTTGCTGGGACTGTCATGCTAATATTTGGTATGGGACTGTATGGATTATTTATCAGTAATGTCCCTCATGACGTGCCTTCCATTGATGATCGTGCTCTTAAGGGCTCTTCTTTATTCGGAATGTTTGCTCTGAAGGAGAGGCCTAAATGGATGAAAATTTGCTCACTTGATGAACTGAAGACGAAGGTGGGACACGTCATTGTTATGATTCTTCTGGTTAAGATGTTTGAGAGGAGCAAGATGGTACAGATAACCACTGGTTTGGATCTGTTGAGTTATTCTGTCTGTATTTTCCTGTCTTCGGCTTCTTTGTACATCCTTCATAATCTGCACAAGGAAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0114

Protein Analysis

346

Amino Acids

37.68

Weight (kDa)

9.65

Isoelectric Point (pI)

49.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0114 PF03350 171 - 309 1.4e-33 Uncharacterized protein family, UPF0114
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 43, 45
AccIII TCCGGA 1 cut(s) 328
AciI CCGC 4 cut(s) 81, 240, 431, 486
AclWI GGATC 1 cut(s) 966
AcsI RAATTY 3 cut(s) 66, 102, 849
AcuI CTGAAG 2 cut(s) 845, 890
AfaI GTAC 2 cut(s) 939, 1007
AfiI CCNNNNNNNGG 7 cut(s) 90, 279, 427, 471, 504, 529, 994
AflII CTTAAG 1 cut(s) 791
AflIII ACRYGT 1 cut(s) 886
AgsI TTSAA 3 cut(s) 439, 503, 677
AhlI ACTAGT 1 cut(s) 626
AjiI CACGTC 2 cut(s) 766, 889
AloI GAACNNNNNNTCC 2 cut(s) 645, 677
AluBI AGCT 1 cut(s) 680
AluI AGCT 1 cut(s) 680
Alw21I GWGCWC 2 cut(s) 317, 790
Alw26I GTCTC 2 cut(s) 330, 370
AlwI GGATC 1 cut(s) 966
Ama87I CYCGRG 1 cut(s) 422
Aor13HI TCCGGA 1 cut(s) 328
AoxI GGCC 4 cut(s) 49, 260, 345, 833
ApeKI GCWGC 1 cut(s) 299
ApoI RAATTY 3 cut(s) 66, 102, 849
AspLEI GCGC 2 cut(s) 45, 96
AspS9I GGNCC 2 cut(s) 260, 346
AsuHPI GGTGA 1 cut(s) 378
AvaI CYCGRG 1 cut(s) 422
BanII GRGCYC 1 cut(s) 800
BbsI GAAGAC 3 cut(s) 486, 878, 984
Bbv12I GWGCWC 2 cut(s) 317, 790
BbvI GCAGC 1 cut(s) 286
BccI CCATC 6 cut(s) 92, 139, 258, 263, 302, 928
BceAI ACGGC 3 cut(s) 247, 360, 472
BcoDI GTCTC 2 cut(s) 330, 370
BcuI ACTAGT 1 cut(s) 626
BfaI CTAG 2 cut(s) 414, 627
BfrI CTTAAG 1 cut(s) 791
BisI GCNGC 2 cut(s) 300, 486
BlsI GCNGC 2 cut(s) 301, 487
BmeT110I CYCGRG 1 cut(s) 422
BmgBI CACGTC 2 cut(s) 766, 889
BmgT120I GGNCC 2 cut(s) 260, 346
BmiI GGNNCC 1 cut(s) 348
BmsI GCATC 1 cut(s) 595
BpiI GAAGAC 3 cut(s) 486, 878, 984
Bsa29I ATCGAT 1 cut(s) 684
BsaWI WCCGGW 3 cut(s) 328, 471, 522
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 7 cut(s) 90, 279, 427, 471, 504, 529, 994
Bse118I RCCGGY 1 cut(s) 427
Bse1I ACTGG 3 cut(s) 626, 655, 955
BseAI TCCGGA 1 cut(s) 328
BseCI ATCGAT 1 cut(s) 684
BseGI GGATG 6 cut(s) 84, 131, 250, 610, 849, 1008
BseLI CCNNNNNNNGG 7 cut(s) 90, 279, 427, 471, 504, 529, 994
BseMII CTCAG 2 cut(s) 121, 480
BseNI ACTGG 3 cut(s) 626, 655, 955
BseRI GAGGAG 6 cut(s) 134, 208, 211, 358, 361, 940
BseXI GCAGC 1 cut(s) 286
BseYI CCCAGC 1 cut(s) 698
BsgI GTGCAG 1 cut(s) 1007
Bsh1236I CGCG 2 cut(s) 43, 45
BshFI GGCC 4 cut(s) 51, 262, 347, 835
BshVI ATCGAT 1 cut(s) 684
BsiHKAI GWGCWC 2 cut(s) 317, 790
BsiHKCI CYCGRG 1 cut(s) 422
BsiSI CCGG 5 cut(s) 135, 329, 428, 472, 523
BslFI GGGAC 6 cut(s) 8, 276, 715, 740, 741, 897
BslI CCNNNNNNNGG 7 cut(s) 90, 279, 427, 471, 504, 529, 994
BsmAI GTCTC 2 cut(s) 330, 370
BsmBI CGTCTC 1 cut(s) 330
BsmFI GGGAC 6 cut(s) 8, 276, 715, 740, 741, 897
BsnI GGCC 4 cut(s) 51, 262, 347, 835
BsoBI CYCGRG 1 cut(s) 422
Bsp1286I GDGCHC 3 cut(s) 317, 790, 800
Bsp13I TCCGGA 1 cut(s) 328
Bsp1407I TGTACA 1 cut(s) 1005
Bsp143I GATC 3 cut(s) 213, 781, 958
BspACI CCGC 4 cut(s) 81, 240, 431, 486
BspANI GGCC 4 cut(s) 51, 262, 347, 835
BspCNI CTCAG 2 cut(s) 120, 481
BspDI ATCGAT 1 cut(s) 684
BspEI TCCGGA 1 cut(s) 328
BspFNI CGCG 2 cut(s) 43, 45
BspHI TCATGA 1 cut(s) 760
BspLI GGNNCC 1 cut(s) 348
BspPI GGATC 1 cut(s) 966
BspQI GCTCTTC 1 cut(s) 805
BspTI CTTAAG 1 cut(s) 791
BsrFI RCCGGY 1 cut(s) 427
BsrGI TGTACA 1 cut(s) 1005
BsrI ACTGG 3 cut(s) 626, 655, 955
BssAI RCCGGY 1 cut(s) 427
BssMI GATC 3 cut(s) 213, 781, 958
Bst4CI ACNGT 6 cut(s) 282, 324, 361, 537, 706, 732
Bst6I CTCTTC 2 cut(s) 209, 805
BstAFI CTTAAG 1 cut(s) 791
BstAPI GCANNNNNTGC 1 cut(s) 539
BstAUI TGTACA 1 cut(s) 1005
BstC8I GCNNGC 2 cut(s) 429, 433
BstDEI CTNAG 2 cut(s) 107, 489
BstF5I GGATG 6 cut(s) 84, 131, 250, 610, 849, 1008
BstFNI CGCG 2 cut(s) 43, 45
BstHHI GCGC 2 cut(s) 45, 96
BstKTI GATC 3 cut(s) 216, 784, 961
BstMAI GTCTC 2 cut(s) 330, 370
BstMBI GATC 3 cut(s) 213, 781, 958
BstMWI GCNNNNNNNGC 3 cut(s) 57, 485, 539
BstUI CGCG 2 cut(s) 43, 45
BstV1I GCAGC 1 cut(s) 286
BstV2I GAAGAC 3 cut(s) 486, 878, 984
BstX2I RGATCY 1 cut(s) 958
BstXI CCANNNNNNTGG 2 cut(s) 401, 955
BstYI RGATCY 1 cut(s) 958
Bsu15I ATCGAT 1 cut(s) 684
BsuRI GGCC 4 cut(s) 51, 262, 347, 835
BsuTUI ATCGAT 1 cut(s) 684
BtgZI GCGATG 1 cut(s) 280
BtrI CACGTC 2 cut(s) 766, 889
BtsCI GGATG 6 cut(s) 84, 131, 250, 610, 849, 1008
BtsIMutI CAGTG 4 cut(s) 375, 533, 648, 948
Cac8I GCNNGC 2 cut(s) 429, 433
CciI TCATGA 1 cut(s) 760
CfoI GCGC 2 cut(s) 45, 96
Cfr10I RCCGGY 1 cut(s) 427
Cfr13I GGNCC 2 cut(s) 260, 346
ClaI ATCGAT 1 cut(s) 684
CseI GACGC 1 cut(s) 396
Csp6I GTAC 2 cut(s) 938, 1006
CviAII CATG 2 cut(s) 709, 761
CviQI GTAC 2 cut(s) 938, 1006
DdeI CTNAG 2 cut(s) 107, 489
DpnI GATC 3 cut(s) 215, 783, 960
DpnII GATC 3 cut(s) 213, 781, 958
Eam1104I CTCTTC 2 cut(s) 209, 805
EarI CTCTTC 2 cut(s) 209, 805
EciI GGCGGA 1 cut(s) 229
Eco147I AGGCCT 1 cut(s) 835
Eco24I GRGCYC 1 cut(s) 800
Eco57I CTGAAG 2 cut(s) 845, 890
Eco88I CYCGRG 1 cut(s) 422
EcoT38I GRGCYC 1 cut(s) 800
Esp3I CGTCTC 1 cut(s) 330
FaeI CATG 2 cut(s) 712, 764
FaqI GGGAC 6 cut(s) 8, 276, 715, 740, 741, 897
FatI CATG 2 cut(s) 708, 760
FauI CCCGC 2 cut(s) 88, 424
FauNDI CATATG 1 cut(s) 657
Fnu4HI GCNGC 2 cut(s) 300, 486
FokI GGATG 6 cut(s) 71, 118, 237, 617, 856, 995
FriOI GRGCYC 1 cut(s) 800
Fsp4HI GCNGC 2 cut(s) 300, 486
FspBI CTAG 2 cut(s) 414, 627
GlaI GCGC 2 cut(s) 44, 95
GluI GCNGC 2 cut(s) 300, 486
GsaI CCCAGC 1 cut(s) 702
HaeIII GGCC 4 cut(s) 51, 262, 347, 835
HapII CCGG 5 cut(s) 135, 329, 428, 472, 523
HgaI GACGC 1 cut(s) 396
HhaI GCGC 2 cut(s) 45, 96
Hin1II CATG 2 cut(s) 712, 764
Hin6I GCGC 2 cut(s) 43, 94
HinP1I GCGC 2 cut(s) 43, 94
HinfI GANTC 3 cut(s) 332, 491, 901
HpaII CCGG 5 cut(s) 135, 329, 428, 472, 523
HphI GGTGA 1 cut(s) 378
Hpy166II GTNNAC 2 cut(s) 619, 691
Hpy188I TCNGA 2 cut(s) 812, 825
Hpy188III TCNNGA 3 cut(s) 329, 414, 761
Hpy8I GTNNAC 2 cut(s) 619, 691
Hpy99I CGWCG 1 cut(s) 268
HpyAV CCTTC 7 cut(s) 41, 45, 780, 820, 871, 1022, 1025
HpyCH4III ACNGT 6 cut(s) 282, 324, 361, 537, 706, 732
HpyCH4IV ACGT 2 cut(s) 765, 888
HpyCH4V TGCA 1 cut(s) 1024
HpyF10VI GCNNNNNNNGC 3 cut(s) 57, 485, 539
HpyF3I CTNAG 2 cut(s) 107, 489
HpySE526I ACGT 2 cut(s) 765, 888
Hsp92II CATG 2 cut(s) 712, 764
HspAI GCGC 2 cut(s) 43, 94
Kpn2I TCCGGA 1 cut(s) 328
KroI GCCGGC 1 cut(s) 427
KroNI GCCGGC 1 cut(s) 429
Kzo9I GATC 3 cut(s) 213, 781, 958
LguI GCTCTTC 1 cut(s) 805
LmnI GCTCC 4 cut(s) 35, 65, 320, 927
Lsp1109I GCAGC 1 cut(s) 286
LweI GCATC 1 cut(s) 595
MaeI CTAG 2 cut(s) 414, 627
MaeII ACGT 2 cut(s) 765, 888
MaeIII GTNAC 1 cut(s) 72
MalI GATC 3 cut(s) 215, 783, 960
MboI GATC 3 cut(s) 213, 781, 958
MboII GAAGA 7 cut(s) 196, 297, 486, 792, 883, 896, 984
MflI RGATCY 1 cut(s) 958
MhlI GDGCHC 3 cut(s) 317, 790, 800
MluCI AATT 3 cut(s) 66, 102, 849
MlyI GAGTC 1 cut(s) 500
MmeI TCCRAC 2 cut(s) 140, 526
MroI TCCGGA 1 cut(s) 328
MroNI GCCGGC 1 cut(s) 427
MseI TTAA 3 cut(s) 399, 792, 912
MslI CAYNNNNRTG 2 cut(s) 399, 896
MspA1I CMGCKG 1 cut(s) 488
MspCI CTTAAG 1 cut(s) 791
MspI CCGG 5 cut(s) 135, 329, 428, 472, 523
MvnI CGCG 2 cut(s) 43, 45
MwoI GCNNNNNNNGC 3 cut(s) 57, 485, 539
NaeI GCCGGC 1 cut(s) 429
NdeI CATATG 1 cut(s) 657
NdeII GATC 3 cut(s) 213, 781, 958
NgoMIV GCCGGC 1 cut(s) 427
NlaIII CATG 2 cut(s) 712, 764
NlaIV GGNNCC 1 cut(s) 348
PagI TCATGA 1 cut(s) 760
PceI AGGCCT 1 cut(s) 835
PciSI GCTCTTC 1 cut(s) 805
PcsI WCGNNNNNNNCGW 1 cut(s) 286
PdiI GCCGGC 1 cut(s) 429
PfeI GAWTC 2 cut(s) 332, 901
PkrI GCNGC 2 cut(s) 301, 487
PleI GAGTC 1 cut(s) 499
PpsI GAGTC 1 cut(s) 499
PspFI CCCAGC 1 cut(s) 698
PspN4I GGNNCC 1 cut(s) 348
PspPI GGNCC 2 cut(s) 260, 346
PsuI RGATCY 1 cut(s) 958
RsaI GTAC 2 cut(s) 939, 1007
RsaNI GTAC 2 cut(s) 938, 1006
RseI CAYNNNNRTG 2 cut(s) 399, 896
SapI GCTCTTC 1 cut(s) 805
SaqAI TTAA 3 cut(s) 399, 792, 912
SatI GCNGC 2 cut(s) 300, 486
Sau3AI GATC 3 cut(s) 213, 781, 958
Sau96I GGNCC 2 cut(s) 260, 346
SchI GAGTC 1 cut(s) 500
SduI GDGCHC 3 cut(s) 317, 790, 800
SetI ASST 8 cut(s) 189, 275, 553, 682, 696, 768, 882, 891
SfaNI GCATC 1 cut(s) 595
SmiMI CAYNNNNRTG 2 cut(s) 399, 896
SmlI CTYRAG 1 cut(s) 791
SmoI CTYRAG 1 cut(s) 791
SpeI ACTAGT 1 cut(s) 626
Sse9I AATT 3 cut(s) 66, 102, 849
SseBI AGGCCT 1 cut(s) 835
SsiI CCGC 4 cut(s) 81, 240, 431, 486
SspI AATATT 2 cut(s) 513, 717
SspMI CTAG 2 cut(s) 414, 627
StuI AGGCCT 1 cut(s) 835
TaaI ACNGT 6 cut(s) 282, 324, 361, 537, 706, 732
TaiI ACGT 2 cut(s) 768, 891
TaqI TCGA 3 cut(s) 212, 266, 684
TasI AATT 3 cut(s) 66, 102, 849
TatI WGTACW 1 cut(s) 1005
TauI GCSGC 1 cut(s) 488
TfiI GAWTC 2 cut(s) 332, 901
Tru1I TTAA 3 cut(s) 399, 792, 912
Tru9I TTAA 3 cut(s) 399, 792, 912
TscAI CASTG 4 cut(s) 382, 540, 655, 955
TseI GCWGC 1 cut(s) 299
TspDTI ATGAA 3 cut(s) 860, 879, 1004
TspGWI ACGGA 1 cut(s) 60
TspRI CASTG 4 cut(s) 382, 540, 655, 955
Vha464I CTTAAG 1 cut(s) 791
XapI RAATTY 3 cut(s) 66, 102, 849
XbaI TCTAGA 1 cut(s) 413
XspI CTAG 2 cut(s) 414, 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.