pycom07g01990

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0006973
Physical Location & Seq
Reverse (-)
1599054 .. 1600403
1350 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g01990.1

Sequence Viewer

Length: 1257 bp
ATGGATGTCGTGCACATAAAAGGTCAATCGAAGATTACTTATTGGGGGCGAGTGACGGATTATTTTCACAGCTACAAAACATTTGCAAGTGATCGAGAGGAGAAGTCACTACTACAACGGTGGTCAACTATTCAACAAGCAACCAAGAAGTTTTGTGAGTATGTTACTCAAGTGGAAGAGAAGCACAAATACAGTGTGAATGGGCAAGATCAGCATTTTTATGCGAAACAGTTATACGAAAAATTGGAAAAGAAAAAATTTCCTTTCGATCATTGTTGGAAGCTGTTGAAGGATGCGCCAAAGTGGTTGGATGATATGCGGAAGCCCAGGAAAAAACCAACAAATAGAGGAAAAGGTACAAGTCTCGCTCCTGAATTCTCTTCTCCTTCTACTTCAGAGAGTTTGATAGGTCTTGTAGAGGACGACGTCCCAAATACTGAATCTGGGAACACAGAGAGGAAGAATGTCCCAAATACTGAATTTGGGAAAAAGGAGAGACTGCAAATAAGGAAGGATGCCCCAGATGCTAATTCTGGGAACAGGGAGAGACTACCTATTGGGAATGATGCCCTGAATAGTGATGTTTGGAATAGGGAGACACCACCGAAAAGGATGGACATCCCAAACATTGGTATTGGGGATATGGACGTCCTAGTTACTGATTTTGGGAATACGAAGAGACCACCCATGAGGAAGAATATCCTGAATTCTGATTTTGGGAGCATGGAGAGACCACCAATAAGCACAGACGTCCAAAACACTGACTTGGAGAACATGGATGCCTCAATTACTGATGTTGGGAACATGGAGAGACCACCATTAAAGAAGGGCATCCCAAATACTGATTCTGGGAACATGGAGAGACCAACGACAATGAGGGACGTCCTGGATACTGAATTTGGGAGCATGGAGAGACTGCCAGTAAGGCTGGTTGAGAAACCGAAGATCGAAGAAAACCAAAAGAATGACGATGCAAATAAGCAAGCCGTGGATATCCTACACATGATGGAGAAATGGAGAGACCAAGTTAATGAAGAGAAAAGGCAATATCGGTTAGAGAAATTAGAGATGCTTAGGGAGAGGCTTGAGATGGATAAGGAGAGGCTTGGGATGGATAAAGAGAGGCTTCGACTCGACCGTGAGGACAAAGAGGAGAGAATAATGTTGATGGATATAAGTATAATGCCTCATCAGCTTCAACAATACTATCATCGACGTCGAATGGAAATTCTTGCTAGAGGAATGGGTGGTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM-associated

Protein Analysis

419

Amino Acids

48.98

Weight (kDa)

7.7

Isoelectric Point (pI)

41.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM-associated PF14303 85 - 173 3.8e-09 No apical meristem-associated C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 5 cut(s) 429, 651, 753, 885, 1219
AccB7I CCANNNNNTGG 1 cut(s) 629
AciI CCGC 1 cut(s) 319
AcsI RAATTY 6 cut(s) 257, 374, 479, 706, 896, 1227
AcuI CTGAAG 1 cut(s) 378
AcyI GRCGYC 5 cut(s) 426, 648, 750, 882, 1216
AfaI GTAC 1 cut(s) 358
AfiI CCNNNNNNNGG 1 cut(s) 629
AgsI TTSAA 3 cut(s) 134, 289, 1199
AjnI CCWGG 2 cut(s) 326, 885
AjuI GAANNNNNNNTTGG 2 cut(s) 829, 861
AluBI AGCT 3 cut(s) 72, 283, 1195
AluI AGCT 3 cut(s) 72, 283, 1195
Alw21I GWGCWC 1 cut(s) 15
Alw44I GTGCAC 1 cut(s) 11
ApaLI GTGCAC 1 cut(s) 11
ApoI RAATTY 6 cut(s) 257, 374, 479, 706, 896, 1227
AspLEI GCGC 1 cut(s) 298
BaeGI GKGCMC 1 cut(s) 15
BarI GAAGNNNNNNTAC 2 cut(s) 173, 205
Bbv12I GWGCWC 1 cut(s) 15
BccI CCATC 5 cut(s) 607, 1000, 1084, 1105, 1162
BceAI ACGGC 1 cut(s) 971
BciT130I CCWGG 2 cut(s) 328, 887
BciVI GTATCC 1 cut(s) 883
BfaI CTAG 2 cut(s) 653, 1236
BfuI GTATCC 1 cut(s) 883
BglI GCCNNNNNGGC 1 cut(s) 925
Bme1390I CCNGG 2 cut(s) 328, 887
BmrFI CCNGG 2 cut(s) 328, 887
BmsI GCATC 8 cut(s) 283, 505, 514, 556, 769, 840, 961, 1059
Bpu10I CCTNAGC 1 cut(s) 1073
BpuEI CTTGAG 2 cut(s) 153, 1106
BsaBI GATNNNNATC 1 cut(s) 617
BsaHI GRCGYC 5 cut(s) 426, 648, 750, 882, 1216
BsaI GGTCTC 5 cut(s) 673, 724, 805, 856, 1014
BsaJI CCNNGG 2 cut(s) 326, 987
Bsc4I CCNNNNNNNGG 1 cut(s) 629
Bse1I ACTGG 1 cut(s) 920
Bse8I GATNNNNATC 1 cut(s) 617
BseBI CCWGG 2 cut(s) 328, 887
BseDI CCNNGG 2 cut(s) 326, 987
BseGI GGATG 9 cut(s) 10, 298, 316, 520, 618, 618, 784, 831, 1116
BseJI GATNNNNATC 1 cut(s) 617
BseLI CCNNNNNNNGG 1 cut(s) 629
BseNI ACTGG 1 cut(s) 920
BseRI GAGGAG 2 cut(s) 113, 1166
BseSI GKGCMC 1 cut(s) 15
Bsh1285I CGRYCG 1 cut(s) 1138
BsiEI CGRYCG 1 cut(s) 1138
BsiHKAI GWGCWC 1 cut(s) 15
BslFI GGGAC 3 cut(s) 413, 452, 893
BslI CCNNNNNNNGG 1 cut(s) 629
BsmFI GGGAC 3 cut(s) 413, 452, 893
Bso31I GGTCTC 5 cut(s) 673, 724, 805, 856, 1014
Bsp1286I GDGCHC 1 cut(s) 15
Bsp143I GATC 4 cut(s) 91, 208, 268, 945
BspACI CCGC 1 cut(s) 319
BspTNI GGTCTC 5 cut(s) 673, 724, 805, 856, 1014
BsrI ACTGG 1 cut(s) 920
BssECI CCNNGG 2 cut(s) 326, 987
BssMI GATC 4 cut(s) 91, 208, 268, 945
BssNI GRCGYC 5 cut(s) 426, 648, 750, 882, 1216
Bst2UI CCWGG 2 cut(s) 328, 887
Bst4CI ACNGT 4 cut(s) 120, 194, 231, 1139
Bst6I CTCTTC 4 cut(s) 171, 385, 671, 1029
BstACI GRCGYC 5 cut(s) 426, 648, 750, 882, 1216
BstC8I GCNNGC 1 cut(s) 984
BstDEI CTNAG 1 cut(s) 1073
BstDSI CCRYGG 1 cut(s) 987
BstF5I GGATG 9 cut(s) 10, 298, 316, 520, 618, 618, 784, 831, 1116
BstHHI GCGC 1 cut(s) 298
BstKTI GATC 4 cut(s) 94, 211, 271, 948
BstMBI GATC 4 cut(s) 91, 208, 268, 945
BstMCI CGRYCG 1 cut(s) 1138
BstMWI GCNNNNNNNGC 4 cut(s) 211, 524, 925, 1192
BstNI CCWGG 2 cut(s) 328, 887
BstSCI CCNGG 2 cut(s) 326, 885
BstSLI GKGCMC 1 cut(s) 15
BsuI GTATCC 1 cut(s) 883
BtgI CCRYGG 1 cut(s) 987
BtsCI GGATG 9 cut(s) 10, 298, 316, 520, 618, 618, 784, 831, 1116
BtsIMutI CAGTG 2 cut(s) 199, 759
Cac8I GCNNGC 1 cut(s) 984
CfoI GCGC 1 cut(s) 298
Csp6I GTAC 1 cut(s) 357
CviAII CATG 7 cut(s) 688, 724, 775, 805, 856, 907, 1003
CviJI RGCY 9 cut(s) 72, 283, 325, 928, 986, 1084, 1105, 1126, 1195
CviKI_1 RGCY 9 cut(s) 72, 283, 325, 928, 986, 1084, 1105, 1126, 1195
CviQI GTAC 1 cut(s) 357
DdeI CTNAG 1 cut(s) 1073
DpnI GATC 4 cut(s) 93, 210, 270, 947
DpnII GATC 4 cut(s) 91, 208, 268, 945
Eam1104I CTCTTC 4 cut(s) 171, 385, 671, 1029
EarI CTCTTC 4 cut(s) 171, 385, 671, 1029
Eco31I GGTCTC 5 cut(s) 673, 724, 805, 856, 1014
Eco32I GATATC 1 cut(s) 994
Eco57I CTGAAG 1 cut(s) 378
EcoRI GAATTC 2 cut(s) 374, 706
EcoRII CCWGG 2 cut(s) 326, 885
EcoRV GATATC 1 cut(s) 994
FaeI CATG 7 cut(s) 691, 727, 778, 808, 859, 910, 1006
FalI AAGNNNNNCTT 2 cut(s) 1110, 1142
FaqI GGGAC 3 cut(s) 413, 452, 893
FatI CATG 7 cut(s) 687, 723, 774, 804, 855, 906, 1002
FokI GGATG 9 cut(s) 17, 305, 323, 527, 605, 625, 791, 818, 1123
FspBI CTAG 2 cut(s) 653, 1236
GlaI GCGC 1 cut(s) 297
HhaI GCGC 1 cut(s) 298
Hin1I GRCGYC 5 cut(s) 426, 648, 750, 882, 1216
Hin1II CATG 7 cut(s) 691, 727, 778, 808, 859, 910, 1006
Hin6I GCGC 1 cut(s) 296
HinP1I GCGC 1 cut(s) 296
HincII GTYRAC 1 cut(s) 126
HindII GTYRAC 1 cut(s) 126
HinfI GANTC 3 cut(s) 440, 845, 1131
Hpy166II GTNNAC 2 cut(s) 13, 126
Hpy188I TCNGA 3 cut(s) 397, 712, 1256
Hpy188III TCNNGA 3 cut(s) 95, 371, 703
Hpy8I GTNNAC 2 cut(s) 13, 126
Hpy99I CGWCG 3 cut(s) 428, 1218, 1221
HpyAV CCTTC 4 cut(s) 283, 396, 505, 820
HpyCH4III ACNGT 4 cut(s) 120, 194, 231, 1139
HpyCH4IV ACGT 5 cut(s) 426, 648, 750, 882, 1216
HpyCH4V TGCA 4 cut(s) 13, 86, 502, 974
HpyF10VI GCNNNNNNNGC 4 cut(s) 211, 524, 925, 1192
HpyF3I CTNAG 1 cut(s) 1073
HpySE526I ACGT 5 cut(s) 426, 648, 750, 882, 1216
Hsp92I GRCGYC 5 cut(s) 426, 648, 750, 882, 1216
Hsp92II CATG 7 cut(s) 691, 727, 778, 808, 859, 910, 1006
HspAI GCGC 1 cut(s) 296
Kzo9I GATC 4 cut(s) 91, 208, 268, 945
LmnI GCTCC 3 cut(s) 373, 720, 903
LweI GCATC 8 cut(s) 283, 505, 514, 556, 769, 840, 961, 1059
MaeI CTAG 2 cut(s) 653, 1236
MaeII ACGT 5 cut(s) 426, 648, 750, 882, 1216
MaeIII GTNAC 4 cut(s) 52, 105, 163, 655
MalI GATC 4 cut(s) 93, 210, 270, 947
MboI GATC 4 cut(s) 91, 208, 268, 945
MboII GAAGA 9 cut(s) 43, 188, 372, 472, 688, 706, 955, 962, 1046
MhlI GDGCHC 1 cut(s) 15
MlyI GAGTC 1 cut(s) 1125
MmeI TCCRAC 2 cut(s) 257, 288
MseI TTAA 2 cut(s) 821, 1029
MslI CAYNNNNRTG 1 cut(s) 219
MspR9I CCNGG 2 cut(s) 328, 887
MvaI CCWGG 2 cut(s) 328, 887
MwoI GCNNNNNNNGC 4 cut(s) 211, 524, 925, 1192
NdeII GATC 4 cut(s) 91, 208, 268, 945
NlaIII CATG 7 cut(s) 691, 727, 778, 808, 859, 910, 1006
NmuCI GTSAC 2 cut(s) 52, 105
PcsI WCGNNNNNNNCGW 1 cut(s) 1135
PfeI GAWTC 2 cut(s) 440, 845
PflFI GACNNNGTC 1 cut(s) 425
PflMI CCANNNNNTGG 1 cut(s) 629
PfoI TCCNGGA 1 cut(s) 885
PleI GAGTC 1 cut(s) 1125
PpsI GAGTC 1 cut(s) 1125
Psp6I CCWGG 2 cut(s) 326, 885
PspGI CCWGG 2 cut(s) 326, 885
PsyI GACNNNGTC 1 cut(s) 425
RsaI GTAC 1 cut(s) 358
RsaNI GTAC 1 cut(s) 357
RseI CAYNNNNRTG 1 cut(s) 219
SaqAI TTAA 2 cut(s) 821, 1029
Sau3AI GATC 4 cut(s) 91, 208, 268, 945
SchI GAGTC 1 cut(s) 1125
ScrFI CCNGG 2 cut(s) 328, 887
SduI GDGCHC 1 cut(s) 15
SfaNI GCATC 8 cut(s) 283, 505, 514, 556, 769, 840, 961, 1059
SmiMI CAYNNNNRTG 1 cut(s) 219
SmlI CTYRAG 2 cut(s) 168, 1085
SmoI CTYRAG 2 cut(s) 168, 1085
SsiI CCGC 1 cut(s) 319
SspMI CTAG 2 cut(s) 653, 1236
StyD4I CCNGG 2 cut(s) 326, 885
TaaI ACNGT 4 cut(s) 120, 194, 231, 1139
TaiI ACGT 5 cut(s) 429, 651, 753, 885, 1219
TaqI TCGA 8 cut(s) 29, 94, 267, 948, 1129, 1134, 1213, 1219
TfiI GAWTC 2 cut(s) 440, 845
Tru1I TTAA 2 cut(s) 821, 1029
Tru9I TTAA 2 cut(s) 821, 1029
TscAI CASTG 2 cut(s) 199, 766
TseFI GTSAC 2 cut(s) 52, 105
Tsp45I GTSAC 2 cut(s) 52, 105
TspDTI ATGAA 1 cut(s) 1047
TspGWI ACGGA 1 cut(s) 71
TspRI CASTG 2 cut(s) 199, 766
Tth111I GACNNNGTC 1 cut(s) 425
Van91I CCANNNNNTGG 1 cut(s) 629
VneI GTGCAC 1 cut(s) 11
XapI RAATTY 6 cut(s) 257, 374, 479, 706, 896, 1227
XspI CTAG 2 cut(s) 653, 1236
ZraI GACGTC 5 cut(s) 427, 649, 751, 883, 1217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.