Rw2G015000

No apical meristem-associated C-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0006973
Physical Location & Seq
Reverse (-)
17257491 .. 17261896
4406 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G015000.1

Sequence Viewer

Length: 963 bp
ATGATTATTGAAGATGAAAGAGTTGAGCGTGCTGCACAAGATGATCTTGAAACTCTTGCAAGGCAATATGCTGAACCAGATATGGATATTGATGATGTTATGGTTTCCCGTAATCACACAATAGACAGCTGTTTGCTTGTTAACAGTCCGATTAGGCCTCCAAGAGGTCCCGGGTTTAATGTAGATGAGGATCGACTCCTTATTAGTGCTTGGTTGAATACTAGTATGGATTCAATACAAGGCAATGATCAAAAGCTGCATACTTTTTGGGGGAAAATTACTGAGTACTATCATCAGTACAAGTCCTTCAACAGTGATCGTTCACAACCGATGCTGACACAAAGGTGGGGAAAAATACAAAAGGTGGTGAACAAATTTTCAGGATGTTTTGCAGCTATAAATGGAAGACATGAAAGTGGAAAGACTGAACAAGACAAGATTGCAGATGCGAAGAAGATGTTTGAAGCACAAGAAAAGAGAAGGTTCACACTTGATCATGCTTGGATTTTGTTAAGGCACCAACCAAAGTGGATACAACTAATGCAAGAGCTGGACAGCAAGAAAAGTAAGCAAAGATCTAAACCTTCTATAACTAATATCTCATCATCTTCAACTACCGGCACTCACATAGTTATTGAAGAAGAAGAAAACACTTGCTTTCCAGTGGCTAGACCACCGGGTAAGAAGGCTGAGAAAAGAAAGTTGAAAGAAACAACTGATCAGTTGGTGGAAATAAAAAAATTACAGCAAGAGAAAAAAGAAAGAGATGAAAAAAAACTAGAAATTATGAAGGAGAAAATTGAAGTTGACAAGGAGAAACTGCGAGTTAGGAAACTTGAGGCTGAGAATAGGAAAATTGAGGCTGGGATGAGAATTATGTCTATGGATACATCTACTATGAATCCGGAGCAAAGGGCATATTATTCCAAACTTCAAATGAAAATTTTGCAAGGTGATATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM-associated

Protein Analysis

320

Amino Acids

37.36

Weight (kDa)

8.86

Isoelectric Point (pI)

53.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM-associated PF14303 160 - 315 3.8e-23 No apical meristem-associated C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 516
AccIII TCCGGA 1 cut(s) 904
AclWI GGATC 1 cut(s) 198
AcsI RAATTY 2 cut(s) 374, 942
AfaI GTAC 2 cut(s) 287, 299
AfiI CCNNNNNNNGG 1 cut(s) 164
AhlI ACTAGT 1 cut(s) 221
AleI CACNNNNGTG 1 cut(s) 343
AluBI AGCT 4 cut(s) 129, 256, 395, 550
AluI AGCT 4 cut(s) 129, 256, 395, 550
AlwI GGATC 1 cut(s) 198
Ama87I CYCGRG 1 cut(s) 170
Aor13HI TCCGGA 1 cut(s) 904
AoxI GGCC 1 cut(s) 155
ApeKI GCWGC 3 cut(s) 32, 256, 392
ApoI RAATTY 2 cut(s) 374, 942
AspS9I GGNCC 1 cut(s) 167
AsuC2I CCSGG 3 cut(s) 171, 172, 678
AsuHPI GGTGA 1 cut(s) 379
AvaI CYCGRG 1 cut(s) 170
AvaII GGWCC 1 cut(s) 167
BanI GGYRCC 1 cut(s) 516
BarI GAAGNNNNNNTAC 2 cut(s) 290, 322
BbsI GAAGAC 1 cut(s) 412
BbvI GCAGC 3 cut(s) 19, 243, 404
BciVI GTATCC 2 cut(s) 525, 880
BclI TGATCA 3 cut(s) 247, 493, 718
BcnI CCSGG 3 cut(s) 171, 172, 678
BcuI ACTAGT 1 cut(s) 221
BfaI CTAG 3 cut(s) 222, 669, 779
BfuI GTATCC 2 cut(s) 525, 880
BglII AGATCT 1 cut(s) 575
BisI GCNGC 3 cut(s) 33, 257, 393
BlsI GCNGC 3 cut(s) 34, 258, 394
BmcAI AGTACT 1 cut(s) 287
Bme1390I CCNGG 3 cut(s) 171, 172, 678
Bme18I GGWCC 1 cut(s) 167
BmeT110I CYCGRG 1 cut(s) 170
BmgT120I GGNCC 1 cut(s) 167
BmiI GGNNCC 2 cut(s) 169, 518
BmrFI CCNGG 3 cut(s) 171, 172, 678
BmsI GCATC 2 cut(s) 321, 436
BpiI GAAGAC 1 cut(s) 412
BpuEI CTTGAG 1 cut(s) 857
BpuMI CCSGG 3 cut(s) 171, 172, 678
BsaBI GATNNNNATC 1 cut(s) 189
BsaJI CCNNGG 1 cut(s) 170
BsaWI WCCGGW 1 cut(s) 904
Bsc4I CCNNNNNNNGG 1 cut(s) 164
Bse118I RCCGGY 1 cut(s) 617
Bse1I ACTGG 1 cut(s) 662
Bse3DI GCAATG 1 cut(s) 250
Bse8I GATNNNNATC 1 cut(s) 189
BseAI TCCGGA 1 cut(s) 904
BseDI CCNNGG 1 cut(s) 170
BseGI GGATG 2 cut(s) 389, 873
BseJI GATNNNNATC 1 cut(s) 189
BseLI CCNNNNNNNGG 1 cut(s) 164
BseMI GCAATG 1 cut(s) 250
BseMII CTCAG 3 cut(s) 273, 681, 834
BseNI ACTGG 1 cut(s) 662
BseXI GCAGC 3 cut(s) 19, 243, 404
BseYI CCCAGC 1 cut(s) 863
BsgI GTGCAG 1 cut(s) 18
BshFI GGCC 1 cut(s) 157
BshNI GGYRCC 1 cut(s) 516
BsiHKCI CYCGRG 1 cut(s) 170
BsiSI CCGG 4 cut(s) 171, 618, 677, 905
BslFI GGGAC 1 cut(s) 153
BslI CCNNNNNNNGG 1 cut(s) 164
BsmFI GGGAC 1 cut(s) 153
BsnI GGCC 1 cut(s) 157
BsoBI CYCGRG 1 cut(s) 170
Bsp13I TCCGGA 1 cut(s) 904
Bsp143I GATC 7 cut(s) 43, 190, 247, 316, 493, 575, 718
BspANI GGCC 1 cut(s) 157
BspCNI CTCAG 3 cut(s) 274, 682, 835
BspEI TCCGGA 1 cut(s) 904
BspLI GGNNCC 2 cut(s) 169, 518
BspPI GGATC 1 cut(s) 198
BspT107I GGYRCC 1 cut(s) 516
BsrDI GCAATG 1 cut(s) 250
BsrFI RCCGGY 1 cut(s) 617
BsrI ACTGG 1 cut(s) 662
BssAI RCCGGY 1 cut(s) 617
BssECI CCNNGG 1 cut(s) 170
BssMI GATC 7 cut(s) 43, 190, 247, 316, 493, 575, 718
Bst4CI ACNGT 2 cut(s) 146, 314
BstC8I GCNNGC 1 cut(s) 30
BstDEI CTNAG 3 cut(s) 282, 690, 843
BstENI CCTNNNNNAGG 1 cut(s) 162
BstF5I GGATG 2 cut(s) 389, 873
BstKTI GATC 7 cut(s) 46, 193, 250, 319, 496, 578, 721
BstMBI GATC 7 cut(s) 43, 190, 247, 316, 493, 575, 718
BstSCI CCNGG 3 cut(s) 169, 170, 676
BstV1I GCAGC 3 cut(s) 19, 243, 404
BstV2I GAAGAC 1 cut(s) 412
BstX2I RGATCY 1 cut(s) 575
BstYI RGATCY 1 cut(s) 575
BsuI GTATCC 2 cut(s) 525, 880
BsuRI GGCC 1 cut(s) 157
BtsCI GGATG 2 cut(s) 389, 873
BtsIMutI CAGTG 2 cut(s) 319, 669
Cac8I GCNNGC 1 cut(s) 30
Cfr10I RCCGGY 1 cut(s) 617
Cfr13I GGNCC 1 cut(s) 167
Cfr9I CCCGGG 1 cut(s) 170
Csp6I GTAC 2 cut(s) 286, 298
CspCI CAANNNNNGTGG 2 cut(s) 509, 544
CviAII CATG 2 cut(s) 410, 497
CviJI RGCY 9 cut(s) 129, 157, 256, 395, 550, 668, 689, 842, 863
CviKI_1 RGCY 9 cut(s) 129, 157, 256, 395, 550, 668, 689, 842, 863
CviQI GTAC 2 cut(s) 286, 298
DdeI CTNAG 3 cut(s) 282, 690, 843
DpnI GATC 7 cut(s) 45, 192, 249, 318, 495, 577, 720
DpnII GATC 7 cut(s) 43, 190, 247, 316, 493, 575, 718
Eco147I AGGCCT 1 cut(s) 157
Eco47I GGWCC 1 cut(s) 167
Eco88I CYCGRG 1 cut(s) 170
EcoNI CCTNNNNNAGG 1 cut(s) 162
EcoO109I RGGNCCY 1 cut(s) 167
FaeI CATG 2 cut(s) 413, 500
FalI AAGNNNNNCTT 2 cut(s) 30, 62
FaqI GGGAC 1 cut(s) 153
FatI CATG 2 cut(s) 409, 496
FbaI TGATCA 3 cut(s) 247, 493, 718
Fnu4HI GCNGC 3 cut(s) 33, 257, 393
FokI GGATG 2 cut(s) 396, 880
Fsp4HI GCNGC 3 cut(s) 33, 257, 393
FspBI CTAG 3 cut(s) 222, 669, 779
GluI GCNGC 3 cut(s) 33, 257, 393
GsaI CCCAGC 1 cut(s) 867
HaeIII GGCC 1 cut(s) 157
HapII CCGG 4 cut(s) 171, 618, 677, 905
Hin1II CATG 2 cut(s) 413, 500
HincII GTYRAC 2 cut(s) 142, 808
HindII GTYRAC 2 cut(s) 142, 808
HinfI GANTC 3 cut(s) 195, 230, 901
HpaI GTTAAC 1 cut(s) 142
HpaII CCGG 4 cut(s) 171, 618, 677, 905
HphI GGTGA 1 cut(s) 379
Hpy166II GTNNAC 5 cut(s) 142, 323, 370, 486, 808
Hpy188I TCNGA 1 cut(s) 150
Hpy188III TCNNGA 3 cut(s) 47, 381, 905
Hpy8I GTNNAC 5 cut(s) 142, 323, 370, 486, 808
HpyAV CCTTC 5 cut(s) 316, 474, 594, 679, 784
HpyCH4III ACNGT 2 cut(s) 146, 314
HpyCH4V TGCA 7 cut(s) 35, 59, 259, 392, 443, 544, 949
HpyF3I CTNAG 3 cut(s) 282, 690, 843
Hsp92II CATG 2 cut(s) 413, 500
Kpn2I TCCGGA 1 cut(s) 904
Ksp22I TGATCA 3 cut(s) 247, 493, 718
KspAI GTTAAC 1 cut(s) 142
Kzo9I GATC 7 cut(s) 43, 190, 247, 316, 493, 575, 718
LmnI GCTCC 1 cut(s) 907
LpnPI CCDG 9 cut(s) 90, 184, 366, 536, 631, 675, 690, 849, 918
Lsp1109I GCAGC 3 cut(s) 19, 243, 404
LweI GCATC 2 cut(s) 321, 436
MaeI CTAG 3 cut(s) 222, 669, 779
MalI GATC 7 cut(s) 45, 192, 249, 318, 495, 577, 720
MboI GATC 7 cut(s) 43, 190, 247, 316, 493, 575, 718
MboII GAAGA 8 cut(s) 23, 417, 463, 466, 600, 650, 653, 656
MflI RGATCY 1 cut(s) 575
MluCI AATT 8 cut(s) 276, 374, 740, 783, 798, 855, 873, 942
MlyI GAGTC 1 cut(s) 189
MnlI CCTC 5 cut(s) 158, 168, 181, 832, 853
MroI TCCGGA 1 cut(s) 904
MseI TTAA 3 cut(s) 141, 177, 512
MslI CAYNNNNRTG 2 cut(s) 343, 414
MspA1I CMGCKG 1 cut(s) 129
MspI CCGG 4 cut(s) 171, 618, 677, 905
MspR9I CCNGG 3 cut(s) 171, 172, 678
NciI CCSGG 3 cut(s) 171, 172, 678
NdeII GATC 7 cut(s) 43, 190, 247, 316, 493, 575, 718
NlaIII CATG 2 cut(s) 413, 500
NlaIV GGNNCC 2 cut(s) 169, 518
OliI CACNNNNGTG 1 cut(s) 343
PceI AGGCCT 1 cut(s) 157
PfeI GAWTC 2 cut(s) 230, 901
PkrI GCNGC 3 cut(s) 34, 258, 394
PleI GAGTC 1 cut(s) 189
PpsI GAGTC 1 cut(s) 189
PpuMI RGGWCCY 1 cut(s) 167
Psp5II RGGWCCY 1 cut(s) 167
PspFI CCCAGC 1 cut(s) 863
PspN4I GGNNCC 2 cut(s) 169, 518
PspPI GGNCC 1 cut(s) 167
PspPPI RGGWCCY 1 cut(s) 167
PsuI RGATCY 1 cut(s) 575
PvuII CAGCTG 1 cut(s) 129
RsaI GTAC 2 cut(s) 287, 299
RsaNI GTAC 2 cut(s) 286, 298
RseI CAYNNNNRTG 2 cut(s) 343, 414
SaqAI TTAA 3 cut(s) 141, 177, 512
SatI GCNGC 3 cut(s) 33, 257, 393
Sau3AI GATC 7 cut(s) 43, 190, 247, 316, 493, 575, 718
Sau96I GGNCC 1 cut(s) 167
ScaI AGTACT 1 cut(s) 287
SchI GAGTC 1 cut(s) 189
ScrFI CCNGG 3 cut(s) 171, 172, 678
SfaNI GCATC 2 cut(s) 321, 436
SinI GGWCC 1 cut(s) 167
SmaI CCCGGG 1 cut(s) 172
SmiMI CAYNNNNRTG 2 cut(s) 343, 414
SmlI CTYRAG 1 cut(s) 836
SmoI CTYRAG 1 cut(s) 836
SpeI ACTAGT 1 cut(s) 221
Sse9I AATT 8 cut(s) 276, 374, 740, 783, 798, 855, 873, 942
SseBI AGGCCT 1 cut(s) 157
SspMI CTAG 3 cut(s) 222, 669, 779
StuI AGGCCT 1 cut(s) 157
StyD4I CCNGG 3 cut(s) 169, 170, 676
TaaI ACNGT 2 cut(s) 146, 314
TaqI TCGA 1 cut(s) 193
TasI AATT 8 cut(s) 276, 374, 740, 783, 798, 855, 873, 942
TatI WGTACW 2 cut(s) 285, 297
TfiI GAWTC 2 cut(s) 230, 901
Tru1I TTAA 3 cut(s) 141, 177, 512
Tru9I TTAA 3 cut(s) 141, 177, 512
TscAI CASTG 2 cut(s) 319, 669
TseI GCWGC 3 cut(s) 32, 256, 392
TspDTI ATGAA 6 cut(s) 30, 426, 783, 803, 914, 953
TspMI CCCGGG 1 cut(s) 170
TspRI CASTG 2 cut(s) 319, 669
VpaK11BI GGWCC 1 cut(s) 167
XagI CCTNNNNNAGG 1 cut(s) 162
XapI RAATTY 2 cut(s) 374, 942
XmaI CCCGGG 1 cut(s) 170
XspI CTAG 3 cut(s) 222, 669, 779
ZrmI AGTACT 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.