Rw2G052400

No apical meristem-associated C-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0006973
Physical Location & Seq
Forward (+)
80370086 .. 80371068
983 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G052400.1

Sequence Viewer

Length: 330 bp
ATGCTCGCCGAGGAGATGCTGTTCTTGATCTTGCATGCGGAAAGGGAGGTGATCTCATCAAATGGGACAAGGCCAAAATTGGGTACTATGTTGGCATTGATATTGCTGAAGGCTCTAATCTTTAGTGCATGTATCATTTTCCAGTGGCAGCTAGTGAAGGAGAAAGTTGAAGTTGACAAGGAGAAACTGCGAGTTAGGAAACTTGAGGCTGAGAATAGGAAATTTGAGGCTGAGATGAAAATTATGTCTATGGATACATCAACTATGAATCCTGAGCAGAGGGCATATTATTCCAAACTTCAGATGAAAATTCTCCAAGGTGATATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM-associated

Protein Analysis

109

Amino Acids

12.71

Weight (kDa)

8.73

Isoelectric Point (pI)

28.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM-associated PF14303 51 - 104 1.4e-06 No apical meristem-associated C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 38
AcsI RAATTY 2 cut(s) 221, 309
AcuI CTGAAG 2 cut(s) 128, 284
AfaI GTAC 1 cut(s) 85
AfiI CCNNNNNNNGG 1 cut(s) 80
AgsI TTSAA 1 cut(s) 170
AloI GAACNNNNNNTCC 1 cut(s) 37
AluBI AGCT 1 cut(s) 151
AluI AGCT 1 cut(s) 151
AoxI GGCC 1 cut(s) 71
ApeKI GCWGC 1 cut(s) 148
ApoI RAATTY 2 cut(s) 221, 309
AsuHPI GGTGA 1 cut(s) 61
BbvI GCAGC 1 cut(s) 160
BciVI GTATCC 1 cut(s) 247
BfaI CTAG 1 cut(s) 152
BfuI GTATCC 1 cut(s) 247
BisI GCNGC 1 cut(s) 149
BlsI GCNGC 1 cut(s) 150
BmsI GCATC 1 cut(s) 6
BplI GAGNNNNNCTC 2 cut(s) 38, 70
Bpu10I CCTNAGC 1 cut(s) 273
BpuEI CTTGAG 1 cut(s) 224
BsaJI CCNNGG 2 cut(s) 9, 316
BsaXI ACNNNNNCTCC 2 cut(s) 5, 35
Bsc4I CCNNNNNNNGG 1 cut(s) 80
Bse1I ACTGG 1 cut(s) 142
BseDI CCNNGG 2 cut(s) 9, 316
BseLI CCNNNNNNNGG 1 cut(s) 80
BseMII CTCAG 3 cut(s) 201, 222, 264
BseNI ACTGG 1 cut(s) 142
BseRI GAGGAG 1 cut(s) 26
BseXI GCAGC 1 cut(s) 160
BshFI GGCC 1 cut(s) 73
BslFI GGGAC 1 cut(s) 79
BslI CCNNNNNNNGG 1 cut(s) 80
BsmFI GGGAC 1 cut(s) 79
BsnI GGCC 1 cut(s) 73
Bsp143I GATC 2 cut(s) 27, 51
BspACI CCGC 1 cut(s) 38
BspANI GGCC 1 cut(s) 73
BspCNI CTCAG 3 cut(s) 202, 223, 265
BsrI ACTGG 1 cut(s) 142
BssECI CCNNGG 2 cut(s) 9, 316
BssMI GATC 2 cut(s) 27, 51
BssT1I CCWWGG 1 cut(s) 316
BstC8I GCNNGC 2 cut(s) 6, 36
BstDEI CTNAG 3 cut(s) 210, 231, 273
BstKTI GATC 2 cut(s) 30, 54
BstMBI GATC 2 cut(s) 27, 51
BstNSI RCATGY 2 cut(s) 38, 132
BstV1I GCAGC 1 cut(s) 160
BsuI GTATCC 1 cut(s) 247
BsuRI GGCC 1 cut(s) 73
BtsIMutI CAGTG 1 cut(s) 149
Cac8I GCNNGC 2 cut(s) 6, 36
Csp6I GTAC 1 cut(s) 84
CviAII CATG 2 cut(s) 35, 129
CviJI RGCY 5 cut(s) 73, 113, 151, 209, 230
CviKI_1 RGCY 5 cut(s) 73, 113, 151, 209, 230
CviQI GTAC 1 cut(s) 84
DdeI CTNAG 3 cut(s) 210, 231, 273
DpnI GATC 2 cut(s) 29, 53
DpnII GATC 2 cut(s) 27, 51
Eco130I CCWWGG 1 cut(s) 316
Eco57I CTGAAG 2 cut(s) 128, 284
EcoT14I CCWWGG 1 cut(s) 316
ErhI CCWWGG 1 cut(s) 316
FaeI CATG 2 cut(s) 38, 132
FaiI YATR 9 cut(s) 36, 89, 130, 245, 251, 266, 286, 326, 328
FaqI GGGAC 1 cut(s) 79
FatI CATG 2 cut(s) 34, 128
Fnu4HI GCNGC 1 cut(s) 149
Fsp4HI GCNGC 1 cut(s) 149
FspBI CTAG 1 cut(s) 152
GluI GCNGC 1 cut(s) 149
HaeIII GGCC 1 cut(s) 73
Hin1II CATG 2 cut(s) 38, 132
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HinfI GANTC 1 cut(s) 268
HphI GGTGA 1 cut(s) 61
Hpy166II GTNNAC 1 cut(s) 175
Hpy188I TCNGA 1 cut(s) 303
Hpy188III TCNNGA 2 cut(s) 25, 272
Hpy8I GTNNAC 1 cut(s) 175
HpyAV CCTTC 2 cut(s) 103, 151
HpyCH4V TGCA 2 cut(s) 34, 128
HpyF3I CTNAG 3 cut(s) 210, 231, 273
Hsp92II CATG 2 cut(s) 38, 132
Kzo9I GATC 2 cut(s) 27, 51
LpnPI CCDG 2 cut(s) 155, 285
Lsp1109I GCAGC 1 cut(s) 160
LweI GCATC 1 cut(s) 6
MaeI CTAG 1 cut(s) 152
MalI GATC 2 cut(s) 29, 53
MboI GATC 2 cut(s) 27, 51
MluCI AATT 4 cut(s) 77, 221, 240, 309
MnlI CCTC 5 cut(s) 4, 40, 199, 220, 273
NdeII GATC 2 cut(s) 27, 51
NlaIII CATG 2 cut(s) 38, 132
NmeAIII GCCGAG 1 cut(s) 34
NspI RCATGY 2 cut(s) 38, 132
PaeI GCATGC 1 cut(s) 38
PfeI GAWTC 1 cut(s) 268
PkrI GCNGC 1 cut(s) 150
RsaI GTAC 1 cut(s) 85
RsaNI GTAC 1 cut(s) 84
SatI GCNGC 1 cut(s) 149
Sau3AI GATC 2 cut(s) 27, 51
SetI ASST 3 cut(s) 51, 153, 322
SfaNI GCATC 1 cut(s) 6
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
SphI GCATGC 1 cut(s) 38
Sse9I AATT 4 cut(s) 77, 221, 240, 309
SsiI CCGC 1 cut(s) 38
SspMI CTAG 1 cut(s) 152
StyI CCWWGG 1 cut(s) 316
TasI AATT 4 cut(s) 77, 221, 240, 309
TfiI GAWTC 1 cut(s) 268
TscAI CASTG 1 cut(s) 149
TseI GCWGC 1 cut(s) 148
TspDTI ATGAA 3 cut(s) 251, 281, 320
TspRI CASTG 1 cut(s) 149
XapI RAATTY 2 cut(s) 221, 309
XceI RCATGY 2 cut(s) 38, 132
XspI CTAG 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.