pycom07g28220

Phosphoenolpyruvate carboxykinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0004544
Physical Location & Seq
Forward (+)
27613269 .. 27615862
2594 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1296 bp
ATGATGAGAATCCTCAACTTCAACTTGGAGCGGTTCATTGAAGGCTCATTCAGTTCAACATCCCTGCCTTTCTTCCTCTCCTCCCAACGCGTGCGGGCACGGCCGGCAGGGACGGAGGCAGGGACAGGGACAGGGAGGGTCCTCGATTTCTCGTGTGGTCTTAGCATGGCTTTGGCCGGCAAAGGTGTAATTGTCGACCACCAAGCTTTTCATAATTTGACCTCCTCTCAGCTTCAGCACAAGGGTGCAACAATTCCAGAATCATTGTCAGGACTTCCACTTCTTGTTAGAGGACATTTCCATGGAATAGGAGCAGCAGCAGCAGCAGGAGGAGGAGCTTCCCAAATTTCCAAGCCACAGTTCACTAAGCTTTTGAAACATGTCACAAGTCATATATCATCCATCTCCAACATTTTTGTTCATGACGGTGCTATCGGTTCATTACCAAAATGTGATGCAAAAGTTCGTTTAATAAGTGATAGTCCGTCTGCTGTGCTCTCACTATCCCGTATTCTCTGGGGGACTTCCACCCGTGCAGTTTCTCACGATTCATGTCCTTTGACAGTTTATGTGGCTACTTCTATAAGTTCAGGAATTGGGGATGTTATTGACCTTGGATCTAAAGGAATTAATGGATTTATAGCTGCTGATATTGAGCGTTCATCACTTGTTTTATGTGGTAAAGCCTTCTCAGATTTAAATGTAACTAAAGAAGCACTAGCGGCCTTGTCTGGACCTGTTATATTTGCACGGGGAGGCCTTCCACTATGTGCAAGATTTGCAGCCAAAGGTACCTTTAAGAGCTGCAGACATCTGTTGGTGCCTAAGGAGGCAGGTGTAATTCTTTCTTCTGAAGGTGTTGCACCATTGTTTCAAACTGGTGGCCAAAATCTGTTCAAGTTCCCATCTGTTGTAGTCCTTGCATCTTCTGACAGTACTGGCGTTATCCCTTCTGTTTCAAAGCTCTCCCCTGGCCAGGCAGCTTATCACTTCTTAGCTGGTTATCAGAATGGGAAGTTCATGCCCGCATACAACAAAGGCCCCTCGTCTATTCACCCAATGGAACTAGCTAAGGCATTTCTGTCCAAGTTGCAAGATGACAAGATCTCATCTTACTTATTGAATGTCAATGGAGAAGAAAAGAATGTAAATGGCAAAGATTTGGCTAATCTGGTGCAATCAACTTTGTCCGAGAACATCCCACCATTTGAAGCTAAAAGTGGGGATCTTGAAGACAAGTACAAGAGCTTCCTGTTGAGCGAATTTCAAGAAGTGCCAGAAGAATTATCGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEPCK_ATP

Protein Analysis

432

Amino Acids

45.5

Weight (kDa)

8.78

Isoelectric Point (pI)

40.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEPCK_ATP PF01293 300 - 374 5e-08 Phosphoenolpyruvate carboxykinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 824
Acc36I ACCTGC 1 cut(s) 824
Acc65I GGTACC 1 cut(s) 791
AccB1I GGYRCC 2 cut(s) 791, 820
AccBSI CCGCTC 1 cut(s) 31
AccI GTMKAC 1 cut(s) 195
AccII CGCG 1 cut(s) 90
AciI CCGC 4 cut(s) 31, 94, 722, 1026
AclWI GGATC 2 cut(s) 625, 1233
AcoI YGGCCR 4 cut(s) 101, 174, 883, 973
AcsI RAATTY 2 cut(s) 345, 1262
AcuI CTGAAG 2 cut(s) 218, 873
AdeI CACNNNGTG 1 cut(s) 770
AfaI GTAC 3 cut(s) 793, 937, 1241
AfiI CCNNNNNNNGG 1 cut(s) 976
AflIII ACRYGT 2 cut(s) 88, 379
AjnI CCWGG 2 cut(s) 970, 975
AjuI GAANNNNNNNTTGG 2 cut(s) 344, 376
AleI CACNNNNGTG 1 cut(s) 243
Alw21I GWGCWC 1 cut(s) 498
AlwI GGATC 2 cut(s) 625, 1233
AoxI GGCC 7 cut(s) 101, 174, 723, 757, 883, 973, 1039
ApeKI GCWGC 8 cut(s) 314, 317, 320, 323, 644, 782, 804, 980
ApoI RAATTY 2 cut(s) 345, 1262
AseI ATTAAT 1 cut(s) 630
Asp718I GGTACC 1 cut(s) 791
AspS9I GGNCC 3 cut(s) 139, 734, 1040
AsuHPI GGTGA 1 cut(s) 1046
AvaII GGWCC 2 cut(s) 139, 734
AxyI CCTNAGG 1 cut(s) 825
BaeGI GKGCMC 1 cut(s) 100
BalI TGGCCA 2 cut(s) 885, 975
BanI GGYRCC 2 cut(s) 791, 820
BarI GAAGNNNNNNTAC 2 cut(s) 1232, 1264
BauI CACGAG 1 cut(s) 151
BbsI GAAGAC 1 cut(s) 1239
Bbv12I GWGCWC 1 cut(s) 498
BbvI GCAGC 8 cut(s) 326, 329, 332, 335, 631, 791, 794, 992
BccI CCATC 2 cut(s) 410, 913
BceAI ACGGC 1 cut(s) 116
BciT130I CCWGG 2 cut(s) 972, 977
BfaI CTAG 2 cut(s) 719, 1067
BfmI CTRYAG 1 cut(s) 805
BfuAI ACCTGC 1 cut(s) 824
BglII AGATCT 1 cut(s) 1104
BisI GCNGC 9 cut(s) 315, 318, 321, 324, 645, 723, 783, 805, 981
BlsI GCNGC 9 cut(s) 316, 319, 322, 325, 646, 724, 784, 806, 982
BmcAI AGTACT 1 cut(s) 937
Bme1390I CCNGG 2 cut(s) 972, 977
Bme18I GGWCC 2 cut(s) 139, 734
BmgT120I GGNCC 3 cut(s) 139, 734, 1040
BmiI GGNNCC 4 cut(s) 140, 793, 822, 1042
BmrFI CCNGG 2 cut(s) 972, 977
BmsI GCATC 2 cut(s) 445, 932
BpiI GAAGAC 1 cut(s) 1239
Bpu10I CCTNAGC 1 cut(s) 1071
BsaBI GATNNNNATC 1 cut(s) 8
BsaJI CCNNGG 3 cut(s) 301, 613, 970
Bsc4I CCNNNNNNNGG 1 cut(s) 976
Bse118I RCCGGY 2 cut(s) 103, 176
Bse1I ACTGG 2 cut(s) 883, 943
Bse21I CCTNAGG 1 cut(s) 825
Bse8I GATNNNNATC 1 cut(s) 8
BseBI CCWGG 2 cut(s) 972, 977
BseDI CCNNGG 3 cut(s) 301, 613, 970
BseGI GGATG 4 cut(s) 59, 398, 607, 1197
BseJI GATNNNNATC 1 cut(s) 8
BseLI CCNNNNNNNGG 1 cut(s) 976
BseMII CTCAG 2 cut(s) 242, 705
BseNI ACTGG 2 cut(s) 883, 943
BseRI GAGGAG 4 cut(s) 70, 214, 345, 348
BseSI GKGCMC 1 cut(s) 100
BseX3I CGGCCG 1 cut(s) 101
BseXI GCAGC 8 cut(s) 326, 329, 332, 335, 631, 791, 794, 992
BsgI GTGCAG 1 cut(s) 555
Bsh1236I CGCG 1 cut(s) 90
Bsh1285I CGRYCG 1 cut(s) 104
BshFI GGCC 7 cut(s) 103, 176, 725, 759, 885, 975, 1041
BshNI GGYRCC 2 cut(s) 791, 820
BsiEI CGRYCG 1 cut(s) 104
BsiHKAI GWGCWC 1 cut(s) 498
BsiSI CCGG 2 cut(s) 104, 177
BslFI GGGAC 4 cut(s) 124, 136, 142, 535
BslI CCNNNNNNNGG 1 cut(s) 976
BsmFI GGGAC 4 cut(s) 124, 136, 142, 535
BsnI GGCC 7 cut(s) 103, 176, 725, 759, 885, 975, 1041
Bsp1286I GDGCHC 2 cut(s) 100, 498
Bsp143I GATC 3 cut(s) 617, 1104, 1225
Bsp19I CCATGG 1 cut(s) 301
BspACI CCGC 4 cut(s) 31, 94, 722, 1026
BspANI GGCC 7 cut(s) 103, 176, 725, 759, 885, 975, 1041
BspCNI CTCAG 2 cut(s) 241, 704
BspFNI CGCG 1 cut(s) 90
BspHI TCATGA 1 cut(s) 421
BspLI GGNNCC 4 cut(s) 140, 793, 822, 1042
BspMAI CTGCAG 1 cut(s) 809
BspMI ACCTGC 1 cut(s) 824
BspPI GGATC 2 cut(s) 625, 1233
BspT107I GGYRCC 2 cut(s) 791, 820
BsrBI CCGCTC 1 cut(s) 31
BsrFI RCCGGY 2 cut(s) 103, 176
BsrI ACTGG 2 cut(s) 883, 943
BssAI RCCGGY 2 cut(s) 103, 176
BssECI CCNNGG 3 cut(s) 301, 613, 970
BssMI GATC 3 cut(s) 617, 1104, 1225
BssSI CACGAG 1 cut(s) 151
BssT1I CCWWGG 2 cut(s) 301, 613
Bst2BI CACGAG 1 cut(s) 151
Bst2UI CCWGG 2 cut(s) 972, 977
Bst4CI ACNGT 4 cut(s) 360, 428, 565, 935
BstAPI GCANNNNNTGC 1 cut(s) 779
BstC8I GCNNGC 5 cut(s) 92, 96, 105, 178, 1026
BstDEI CTNAG 7 cut(s) 161, 228, 366, 691, 825, 994, 1071
BstDSI CCRYGG 1 cut(s) 301
BstF5I GGATG 4 cut(s) 59, 398, 607, 1197
BstFNI CGCG 1 cut(s) 90
BstKTI GATC 3 cut(s) 620, 1107, 1228
BstMBI GATC 3 cut(s) 617, 1104, 1225
BstMCI CGRYCG 1 cut(s) 104
BstMWI GCNNNNNNNGC 6 cut(s) 100, 104, 320, 323, 722, 779
BstNI CCWGG 2 cut(s) 972, 977
BstNSI RCATGY 1 cut(s) 383
BstSCI CCNGG 2 cut(s) 970, 975
BstSFI CTRYAG 1 cut(s) 805
BstSLI GKGCMC 1 cut(s) 100
BstUI CGCG 1 cut(s) 90
BstV1I GCAGC 8 cut(s) 326, 329, 332, 335, 631, 791, 794, 992
BstV2I GAAGAC 1 cut(s) 1239
BstX2I RGATCY 3 cut(s) 617, 1104, 1225
BstYI RGATCY 3 cut(s) 617, 1104, 1225
BstZI CGGCCG 1 cut(s) 101
Bsu36I CCTNAGG 1 cut(s) 825
BsuRI GGCC 7 cut(s) 103, 176, 725, 759, 885, 975, 1041
BtgI CCRYGG 1 cut(s) 301
BtsCI GGATG 4 cut(s) 59, 398, 607, 1197
BveI ACCTGC 1 cut(s) 824
Cac8I GCNNGC 5 cut(s) 92, 96, 105, 178, 1026
CciI TCATGA 1 cut(s) 421
Cfr10I RCCGGY 2 cut(s) 103, 176
Cfr13I GGNCC 3 cut(s) 139, 734, 1040
Csp6I GTAC 3 cut(s) 792, 936, 1240
CviAII CATG 6 cut(s) 166, 302, 380, 422, 552, 1021
CviQI GTAC 3 cut(s) 792, 936, 1240
DdeI CTNAG 7 cut(s) 161, 228, 366, 691, 825, 994, 1071
DpnI GATC 3 cut(s) 619, 1106, 1227
DpnII GATC 3 cut(s) 617, 1104, 1225
DraI TTTAAA 1 cut(s) 699
DraIII CACNNNGTG 1 cut(s) 770
EaeI YGGCCR 4 cut(s) 101, 174, 883, 973
EagI CGGCCG 1 cut(s) 101
EclXI CGGCCG 1 cut(s) 101
Eco130I CCWWGG 2 cut(s) 301, 613
Eco147I AGGCCT 1 cut(s) 759
Eco47I GGWCC 2 cut(s) 139, 734
Eco52I CGGCCG 1 cut(s) 101
Eco57I CTGAAG 2 cut(s) 218, 873
Eco81I CCTNAGG 1 cut(s) 825
EcoO109I RGGNCCY 2 cut(s) 139, 1040
EcoRII CCWGG 2 cut(s) 970, 975
EcoT14I CCWWGG 2 cut(s) 301, 613
ErhI CCWWGG 2 cut(s) 301, 613
FaeI CATG 6 cut(s) 169, 305, 383, 425, 555, 1024
FaqI GGGAC 4 cut(s) 124, 136, 142, 535
FatI CATG 6 cut(s) 165, 301, 379, 421, 551, 1020
FauI CCCGC 2 cut(s) 87, 1033
FblI GTMKAC 1 cut(s) 195
Fnu4HI GCNGC 9 cut(s) 315, 318, 321, 324, 645, 723, 783, 805, 981
FokI GGATG 4 cut(s) 46, 385, 614, 1184
Fsp4HI GCNGC 9 cut(s) 315, 318, 321, 324, 645, 723, 783, 805, 981
FspBI CTAG 2 cut(s) 719, 1067
GluI GCNGC 9 cut(s) 315, 318, 321, 324, 645, 723, 783, 805, 981
HaeIII GGCC 7 cut(s) 103, 176, 725, 759, 885, 975, 1041
HapII CCGG 2 cut(s) 104, 177
Hin1II CATG 6 cut(s) 169, 305, 383, 425, 555, 1024
HincII GTYRAC 1 cut(s) 196
HindII GTYRAC 1 cut(s) 196
HindIII AAGCTT 2 cut(s) 204, 368
HinfI GANTC 3 cut(s) 9, 260, 548
HpaII CCGG 2 cut(s) 104, 177
HphI GGTGA 1 cut(s) 1046
Hpy166II GTNNAC 2 cut(s) 196, 363
Hpy188I TCNGA 5 cut(s) 694, 853, 931, 1008, 1192
Hpy188III TCNNGA 8 cut(s) 257, 270, 422, 545, 591, 732, 1229, 1268
Hpy8I GTNNAC 2 cut(s) 196, 363
HpyAV CCTTC 5 cut(s) 35, 697, 770, 848, 960
HpyCH4III ACNGT 4 cut(s) 360, 428, 565, 935
HpyF10VI GCNNNNNNNGC 6 cut(s) 100, 104, 320, 323, 722, 779
HpyF3I CTNAG 7 cut(s) 161, 228, 366, 691, 825, 994, 1071
Hsp92II CATG 6 cut(s) 169, 305, 383, 425, 555, 1024
KpnI GGTACC 1 cut(s) 795
KroI GCCGGC 2 cut(s) 103, 176
KroNI GCCGGC 2 cut(s) 105, 178
Kzo9I GATC 3 cut(s) 617, 1104, 1225
LmnI GCTCC 3 cut(s) 28, 311, 335
Lsp1109I GCAGC 8 cut(s) 326, 329, 332, 335, 631, 791, 794, 992
LweI GCATC 2 cut(s) 445, 932
MaeI CTAG 2 cut(s) 719, 1067
MaeIII GTNAC 2 cut(s) 382, 703
MalI GATC 3 cut(s) 619, 1106, 1227
MbiI CCGCTC 1 cut(s) 31
MboI GATC 3 cut(s) 617, 1104, 1225
MboII GAAGA 6 cut(s) 64, 840, 918, 1148, 1244, 1292
MflI RGATCY 3 cut(s) 617, 1104, 1225
MhlI GDGCHC 2 cut(s) 100, 498
MlsI TGGCCA 2 cut(s) 885, 975
MluCI AATT 9 cut(s) 189, 214, 252, 345, 594, 627, 840, 1262, 1283
MluI ACGCGT 1 cut(s) 88
MluNI TGGCCA 2 cut(s) 885, 975
MmeI TCCRAC 1 cut(s) 432
Mox20I TGGCCA 2 cut(s) 885, 975
MroNI GCCGGC 2 cut(s) 103, 176
MscI TGGCCA 2 cut(s) 885, 975
MseI TTAA 4 cut(s) 470, 630, 698, 798
MslI CAYNNNNRTG 3 cut(s) 243, 300, 426
Msp20I TGGCCA 2 cut(s) 885, 975
MspI CCGG 2 cut(s) 104, 177
MspR9I CCNGG 2 cut(s) 972, 977
MvaI CCWGG 2 cut(s) 972, 977
MvnI CGCG 1 cut(s) 90
MwoI GCNNNNNNNGC 6 cut(s) 100, 104, 320, 323, 722, 779
NaeI GCCGGC 2 cut(s) 105, 178
NcoI CCATGG 1 cut(s) 301
NdeII GATC 3 cut(s) 617, 1104, 1225
NgoMIV GCCGGC 2 cut(s) 103, 176
NlaIII CATG 6 cut(s) 169, 305, 383, 425, 555, 1024
NlaIV GGNNCC 4 cut(s) 140, 793, 822, 1042
NmuCI GTSAC 1 cut(s) 382
NspI RCATGY 1 cut(s) 383
OliI CACNNNNGTG 1 cut(s) 243
PagI TCATGA 1 cut(s) 421
PaqCI CACCTGC 1 cut(s) 824
PceI AGGCCT 1 cut(s) 759
PciI ACATGT 1 cut(s) 379
PdiI GCCGGC 2 cut(s) 105, 178
PfeI GAWTC 3 cut(s) 9, 260, 548
PkrI GCNGC 9 cut(s) 316, 319, 322, 325, 646, 724, 784, 806, 982
PpuMI RGGWCCY 1 cut(s) 139
PscI ACATGT 1 cut(s) 379
PshBI ATTAAT 1 cut(s) 630
Psp5II RGGWCCY 1 cut(s) 139
Psp6I CCWGG 2 cut(s) 970, 975
PspGI CCWGG 2 cut(s) 970, 975
PspN4I GGNNCC 4 cut(s) 140, 793, 822, 1042
PspPI GGNCC 3 cut(s) 139, 734, 1040
PspPPI RGGWCCY 1 cut(s) 139
PstI CTGCAG 1 cut(s) 809
PsuI RGATCY 3 cut(s) 617, 1104, 1225
RsaI GTAC 3 cut(s) 793, 937, 1241
RsaNI GTAC 3 cut(s) 792, 936, 1240
RseI CAYNNNNRTG 3 cut(s) 243, 300, 426
SalI GTCGAC 1 cut(s) 194
SaqAI TTAA 4 cut(s) 470, 630, 698, 798
SatI GCNGC 9 cut(s) 315, 318, 321, 324, 645, 723, 783, 805, 981
Sau3AI GATC 3 cut(s) 617, 1104, 1225
Sau96I GGNCC 3 cut(s) 139, 734, 1040
ScaI AGTACT 1 cut(s) 937
ScrFI CCNGG 2 cut(s) 972, 977
SduI GDGCHC 2 cut(s) 100, 498
SfaNI GCATC 2 cut(s) 445, 932
SfcI CTRYAG 1 cut(s) 805
SinI GGWCC 2 cut(s) 139, 734
SmiI ATTTAAAT 1 cut(s) 699
SmiMI CAYNNNNRTG 3 cut(s) 243, 300, 426
Sse9I AATT 9 cut(s) 189, 214, 252, 345, 594, 627, 840, 1262, 1283
SseBI AGGCCT 1 cut(s) 759
SsiI CCGC 4 cut(s) 31, 94, 722, 1026
SspMI CTAG 2 cut(s) 719, 1067
StuI AGGCCT 1 cut(s) 759
StyD4I CCNGG 2 cut(s) 970, 975
StyI CCWWGG 2 cut(s) 301, 613
SwaI ATTTAAAT 1 cut(s) 699
TaaI ACNGT 4 cut(s) 360, 428, 565, 935
TaqI TCGA 2 cut(s) 144, 195
TasI AATT 9 cut(s) 189, 214, 252, 345, 594, 627, 840, 1262, 1283
TatI WGTACW 2 cut(s) 935, 1239
TauI GCSGC 1 cut(s) 725
TfiI GAWTC 3 cut(s) 9, 260, 548
Tru1I TTAA 4 cut(s) 470, 630, 698, 798
Tru9I TTAA 4 cut(s) 470, 630, 698, 798
TseFI GTSAC 1 cut(s) 382
TseI GCWGC 8 cut(s) 314, 317, 320, 323, 644, 782, 804, 980
Tsp45I GTSAC 1 cut(s) 382
TspDTI ATGAA 7 cut(s) 25, 200, 410, 429, 540, 651, 1009
TspGWI ACGGA 2 cut(s) 128, 474
VpaK11BI GGWCC 2 cut(s) 139, 734
VspI ATTAAT 1 cut(s) 630
XapI RAATTY 2 cut(s) 345, 1262
XceI RCATGY 1 cut(s) 383
XmiI GTMKAC 1 cut(s) 195
XspI CTAG 2 cut(s) 719, 1067
ZrmI AGTACT 1 cut(s) 937
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.