pycom08g03840

Belongs to the helicase family. Dicer subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0001909
Physical Location & Seq
Forward (+)
2924288 .. 2930103
5816 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3036 bp
ATGGGTGCCCTGAATGACCATCTTCTCCCAGTCATTGAAGTGCCCGATGTGAAAAGAAAAGAGGAAGCTTCTGGTGCAGGAACTACTAAAAGGAAGGAACTACATGGTACAACTGGCATTCGTATGCTATCTGGAACTTGGGGGGAGAAACTTGATGGTGCTCTTTTTCAGGCATATAAGTTTGACTTCTCCTGTAATATTGCCAGTGAGTTGTATTCTGGATTTGTTTTTCTCATTGAATCAAAGCTTGCTGATGATGTGGGGAATATCAAATTGGATCTTTACCTGCTTTCGAAGACAGTTAAGGCTTCTGTTTCTTCCGGTGGGGAGGTTCATTTGGACGGGGAACAGATGGAAAAAGCAAAGTGCTTCCAGGAATTTTTCTTTAATGGATTGTTTGGGAAATTGTATGGAACCAAATCAGCTAGAAAACAGAGAGAACTTTTACTGGAGAAAGAAACAAGAAAACTGTGGACTCCATCATGCTTATATTTGCTTCTTCCGGTTGAGACATTGAATGATTCAAAAGATGAATCCTGGAGAATAAATTGGGCAGGGATCAATTCTTGTGTTTCTGTGGTAGAGTTCTTAAAGAGAAACTCGTCTTTGGGCAGTCACCAGTGTAACCTTGACACAGGGAATTCAGTTCCAAGTAGAACTGTCTCATCTGAGACTGAGTGCAGCAGTGCGGGGATAGTTCACTTTGCTAATTGCTCTGTTCATGTAAAAAATCTCAAAGATACGGTGGTGGTGGCTATCCATACTGGAAGAATCTACTCAGTTTATGAGGTTATGAGTAACACTTCGGCTGAGAGTCCTTTTGATGGAAAGAAAGACAATGTACCATCACAATATACTTCATTTTCTGAGTACTTCAACAAAAAATATGGGATTGTGCTCCTATATCCTGGTCAGCCTCTGTTGCGACTGAAACAAAGTCATAATCCGCACAACCTTCTTGTGAACTTCAATGGTGAAGGTGGTAATGGTAAGACATTGCAATCTGGTGTTGTTATTGGAAGGCAGCGCATGTATGCTCACATGCCACCTGAGCTTCTAGTCACTATTGGTGTCCCAAGAGACGTTCTAAAGTCATGTTATTTACTACCATCATTGATTCATCGCTTGGAGTCCCTGATGCTAGCCAGTCAACTTAGAGAAGAGATTAATTATCATTGTAGCAGCTTTGGGATATCAAGCTCATTGATTCTAGAAGCACTTACAACACTAAGATGTAATGATGATTTTTCTATGGAGCGCCTGGAATTGCTTGGTGATTCAATTTTAAAATATACTGTGAGCTGCCACCTCTTTCTCAAATATCCCAAAAAGCATGAAGGACAGTTAACTAACTATCGCATGCTGACTATTTGTAACTCAAACCTACATAGATTGGGAATGAATCGCAAGTTACAGGGATATATCCGTGATGGTGCATTTGACCCATGTCGTTGGGTTGCTCCAGGGCAGATTTCTCTACGACCCGTTCCTTGTAATTGTGGAGTGGAGACTTTAGAAGTACCATTAGATAGCAAATTCCAAACCGAAGACCCGAAAGTTGTGGTTGGAAAGAGCTGTGATAAGGGCCATAGATGGATGTGTTCAAAAACAATCTCTGATTGTGTTGAAGCCCTTATAGGAGCATACTATGTTGCTGGTGGATTGCCTGCTACCTTGCATGTGATGAAGTGGCTCGATATTGATGTAGAACTTGAACCTTCCTTGGTTGCTGATGCAATTATGACGGCATCTCTTCATTCGTACACCTCGAAAGCTAATGAGATTGCAGTCTTGGAGTCAAAGCTTCACTATGAATTTTCTACCAAGGGTCTCTTACTGGAGGCCATGACACATGCATCTGACCAAGAATCAGGAGGTGGCTACTGTTATGAGCGACTTGAATTTCTAGGCGACTCTGTGTTGGACCTGCTTATCACAAGGCATCTCTATCATAGCCACACAGATATCGATCCAGGCGAGTTAACTGACTTGCGCTCAGCTTCTGTTAATAATGAAAGTTTTGCGCAAGTTGCTGTTAGACATGACCTCCAGCAGCATCTGCAACATGCATCGATGTTACTTCTAAGTCAGATAACAGAATATGTGAAGTCTCTTTCAGAACCTGACAATGGTACCACACTTCAAGGCACCAAAGGTCCTAAGGCTCTTGGAGACATGGTGGAAAGTATTGCAGGGGCTATTCTAATTGATACCAAACTTAATCTTGATGAAGTGTGGAGAATTTTTGAGCCATTATTATCTCCAATTGTGACTCCTGACAAACTTGAGCTGCCTCCACTACGGGAGCTGATTGAATTATGCGACTTGTTCGGGTACTATAAAAAAGAGAAGTGTATCAATAAGGGTGAAACAGTTCATGCGGAGCTGACTGTACAACTGAAAGATGTTCTGTTGACTGGAGAGGGGTGCGATCGGACTAAAAAAGGTGCAAAAGGAGAAGCAGCTCGTCGTTTACTCAAGAAACTTGAGGAGAGAGGTATTTCATTTTCCAAGAAGAGGAAACTGGATGCTGACCAATTAGGTGATTCCTCTTCCCTTGACATCAACCATAAGGTCAGCGACCAAGTAACTGTCAAAGATTCTTCAGCGTTAATGGTCTATAAAAGGCGAAAGACAACTGAAATGCCTCTAGCTGCTCCACCAACTGGAGTTTCTCCTTCAGTAATTGCCAGGAAAACCTCTAGCCCCAACAATGCCGCCCCAGTCATTAAAGCAATCAACATGAAGAAAGGAGGGCCTCGATCTGCTCTTTTTGCTCTGTGCATGAAACAGCAGTGGCCAAAGCCAGGCTTTAAAATAACAGAAACTCAGTCAAGAACTCCAATCACATTTGCTGAAAGCTCAACCAAGTACTTCAACAGTTTTGTCGCACAAGTAACCTTGCACATACCCAACTCCGGCGTCATTGAATGCACAGGAGATGCTAGACCTGACAAGGGGAGTTCAGAAGATTCTGCAGCCGTCGTCTTGTTTCAAGAGCTTCAACGGCAGGGAAGAGTTGTAATTGGTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003725 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006259 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009892 GO:0009987 GO:0010033 GO:0010216 GO:0010267 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0031974 GO:0031981 GO:0032296 GO:0032991 GO:0034641 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051214 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0098542 GO:0098586 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko05206 map05206
Pfam Domains
Protein Families
DEAD Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1012

Amino Acids

111.92

Weight (kDa)

7.14

Isoelectric Point (pI)

41.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAZ PF02170 243 - 354 1.1e-10 PAZ domain
Ribonuclease_3 PF00636 419 - 554 5.9e-18 Ribonuclease III domain
Ribonucleas_3_3 PF14622 419 - 476 7.3e-06 Ribonuclease-III-like
Ribonucleas_3_3 PF14622 610 - 737 3.3e-15 Ribonuclease-III-like
Ribonuclease_3 PF00636 631 - 738 7.2e-22 Ribonuclease III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2025
Acc36I ACCTGC 2 cut(s) 294, 1935
Acc65I GGTACC 1 cut(s) 2132
AccB1I GGYRCC 3 cut(s) 5, 2132, 2147
AccB7I CCANNNNNTGG 1 cut(s) 2666
AciI CCGC 4 cut(s) 689, 947, 2381, 2718
AclWI GGATC 3 cut(s) 285, 566, 1964
AcoI YGGCCR 1 cut(s) 2798
AcsI RAATTY 6 cut(s) 377, 640, 1535, 1814, 1901, 2241
AcuI CTGAAG 2 cut(s) 2589, 2664
AcyI GRCGYC 1 cut(s) 2922
AfaI GTAC 9 cut(s) 109, 843, 872, 1521, 1763, 2134, 2336, 2394, 2873
AfiI CCNNNNNNNGG 7 cut(s) 824, 2129, 2517, 2666, 2807, 2918, 2957
AjnI CCWGG 8 cut(s) 372, 536, 907, 1260, 1462, 1972, 2690, 2806
AjuI GAANNNNNNNTTGG 4 cut(s) 257, 289, 2656, 2688
Alw21I GWGCWC 2 cut(s) 163, 900
Alw26I GTCTC 8 cut(s) 503, 665, 667, 1074, 1502, 1835, 2115, 2166
AlwI GGATC 3 cut(s) 285, 566, 1964
AlwNI CAGNNNCTG 4 cut(s) 919, 2003, 2059, 2123
AoxI GGCC 4 cut(s) 1585, 1842, 2756, 2798
ApeKI GCWGC 9 cut(s) 681, 1024, 1182, 1302, 2053, 2290, 2462, 2654, 2978
ApoI RAATTY 6 cut(s) 377, 640, 1535, 1814, 1901, 2241
AseI ATTAAT 1 cut(s) 1167
Asp700I GAANNNNTTC 1 cut(s) 2373
Asp718I GGTACC 1 cut(s) 2132
AspLEI GCGC 4 cut(s) 1029, 1260, 1995, 2026
AspS9I GGNCC 4 cut(s) 1585, 1924, 2156, 2756
AsuHPI GGTGA 5 cut(s) 608, 986, 1286, 2378, 2555
AsuII TTCGAA 1 cut(s) 293
AsuNHI GCTAGC 1 cut(s) 1141
AvaII GGWCC 2 cut(s) 1924, 2156
AxyI CCTNAGG 1 cut(s) 2160
BaeGI GKGCMC 2 cut(s) 10, 45
BaeI ACNNNNGTAYC 4 cut(s) 732, 765, 2116, 2149
BalI TGGCCA 1 cut(s) 2800
BanI GGYRCC 3 cut(s) 5, 2132, 2147
BbsI GAAGAC 2 cut(s) 302, 1554
Bbv12I GWGCWC 2 cut(s) 163, 900
BbvI GCAGC 9 cut(s) 693, 1036, 1194, 1289, 2065, 2277, 2474, 2641, 2990
BccI CCATC 9 cut(s) 27, 149, 346, 487, 818, 853, 1117, 1424, 1587
BceAI ACGGC 3 cut(s) 1761, 2966, 3023
BciT130I CCWGG 8 cut(s) 374, 538, 909, 1262, 1464, 1974, 2692, 2808
BcoDI GTCTC 8 cut(s) 503, 665, 667, 1074, 1502, 1835, 2115, 2166
BfaI CTAG 8 cut(s) 426, 1058, 1142, 1211, 1907, 2651, 2703, 2946
BfmI CTRYAG 1 cut(s) 2976
BfoI RGCGCY 1 cut(s) 1261
BfuAI ACCTGC 2 cut(s) 294, 1935
BlpI GCTNAGC 1 cut(s) 1996
BmcAI AGTACT 2 cut(s) 872, 2873
Bme1390I CCNGG 8 cut(s) 374, 538, 909, 1262, 1464, 1974, 2692, 2808
Bme18I GGWCC 2 cut(s) 1924, 2156
BmgT120I GGNCC 4 cut(s) 1585, 1924, 2156, 2756
BmiI GGNNCC 4 cut(s) 7, 415, 2134, 2149
BmrFI CCNGG 8 cut(s) 374, 538, 909, 1262, 1464, 1974, 2692, 2808
BmrI ACTGGG 2 cut(s) 23, 2717
BmsI GCATC 9 cut(s) 1128, 1723, 1757, 1865, 1951, 2065, 2078, 2518, 2932
BmtI GCTAGC 1 cut(s) 1145
BmuI ACTGGG 2 cut(s) 23, 2717
BoxI GACNNNNGTC 1 cut(s) 1446
BpiI GAAGAC 2 cut(s) 302, 1554
BpmI CTGGAG 7 cut(s) 470, 559, 1446, 1859, 2033, 2439, 2688
Bpu10I CCTNAGC 1 cut(s) 1050
Bpu1102I GCTNAGC 1 cut(s) 1996
Bpu14I TTCGAA 1 cut(s) 293
BpuEI CTTGAG 3 cut(s) 2306, 2462, 2507
Bsa29I ATCGAT 2 cut(s) 1968, 2072
BsaBI GATNNNNATC 1 cut(s) 1968
BsaHI GRCGYC 1 cut(s) 2922
BsaI GGTCTC 1 cut(s) 1835
BsaJI CCNNGG 3 cut(s) 1463, 1722, 1824
BsaWI WCCGGW 2 cut(s) 320, 502
BsaXI ACNNNNNCTCC 4 cut(s) 2031, 2061, 2163, 2193
Bsc4I CCNNNNNNNGG 7 cut(s) 824, 2129, 2517, 2666, 2807, 2918, 2957
Bse21I CCTNAGG 1 cut(s) 2160
Bse3DI GCAATG 1 cut(s) 995
Bse8I GATNNNNATC 1 cut(s) 1968
BseBI CCWGG 8 cut(s) 374, 538, 909, 1262, 1464, 1974, 2692, 2808
BseCI ATCGAT 2 cut(s) 1968, 2072
BseDI CCNNGG 3 cut(s) 1463, 1722, 1824
BseGI GGATG 2 cut(s) 1602, 2533
BseJI GATNNNNATC 1 cut(s) 1968
BseLI CCNNNNNNNGG 7 cut(s) 824, 2129, 2517, 2666, 2807, 2918, 2957
BseMI GCAATG 1 cut(s) 995
BseMII CTCAG 8 cut(s) 660, 666, 792, 801, 858, 1041, 2010, 2843
BseRI GAGGAG 1 cut(s) 2504
BseSI GKGCMC 2 cut(s) 10, 45
BseXI GCAGC 9 cut(s) 693, 1036, 1194, 1289, 2065, 2277, 2474, 2641, 2990
BsgI GTGCAG 2 cut(s) 96, 700
Bsh1285I CGRYCG 1 cut(s) 2434
BshFI GGCC 4 cut(s) 1587, 1844, 2758, 2800
BshNI GGYRCC 3 cut(s) 5, 2132, 2147
BshVI ATCGAT 2 cut(s) 1968, 2072
BsiEI CGRYCG 1 cut(s) 2434
BsiHKAI GWGCWC 2 cut(s) 163, 900
BsiSI CCGG 3 cut(s) 321, 503, 2919
BslFI GGGAC 2 cut(s) 1058, 1117
BslI CCNNNNNNNGG 7 cut(s) 824, 2129, 2517, 2666, 2807, 2918, 2957
BsmAI GTCTC 8 cut(s) 503, 665, 667, 1074, 1502, 1835, 2115, 2166
BsmBI CGTCTC 1 cut(s) 1074
BsmFI GGGAC 2 cut(s) 1058, 1117
BsmI GAATGC 2 cut(s) 117, 2936
BsnI GGCC 4 cut(s) 1587, 1844, 2758, 2800
Bso31I GGTCTC 1 cut(s) 1835
Bsp119I TTCGAA 1 cut(s) 293
Bsp1286I GDGCHC 4 cut(s) 10, 45, 163, 900
Bsp1407I TGTACA 1 cut(s) 2392
Bsp143I GATC 5 cut(s) 277, 558, 1969, 2431, 2762
Bsp1720I GCTNAGC 1 cut(s) 1996
BspACI CCGC 4 cut(s) 689, 947, 2381, 2718
BspANI GGCC 4 cut(s) 1587, 1844, 2758, 2800
BspCNI CTCAG 8 cut(s) 661, 667, 791, 802, 859, 1042, 2009, 2842
BspDI ATCGAT 2 cut(s) 1968, 2072
BspLI GGNNCC 4 cut(s) 7, 415, 2134, 2149
BspMAI CTGCAG 1 cut(s) 2980
BspMI ACCTGC 2 cut(s) 294, 1935
BspOI GCTAGC 1 cut(s) 1145
BspPI GGATC 3 cut(s) 285, 566, 1964
BspT104I TTCGAA 1 cut(s) 293
BspT107I GGYRCC 3 cut(s) 5, 2132, 2147
BspTNI GGTCTC 1 cut(s) 1835
BsrDI GCAATG 1 cut(s) 995
BsrGI TGTACA 1 cut(s) 2392
BssECI CCNNGG 3 cut(s) 1463, 1722, 1824
BssMI GATC 5 cut(s) 277, 558, 1969, 2431, 2762
BssNI GRCGYC 1 cut(s) 2922
BssT1I CCWWGG 2 cut(s) 1722, 1824
Bst2UI CCWGG 8 cut(s) 374, 538, 909, 1262, 1464, 1974, 2692, 2808
Bst6I CTCTTC 5 cut(s) 1155, 1758, 2510, 2557, 3010
BstACI GRCGYC 1 cut(s) 2922
BstAPI GCANNNNNTGC 1 cut(s) 2059
BstAUI TGTACA 1 cut(s) 2392
BstBI TTCGAA 1 cut(s) 293
BstC8I GCNNGC 4 cut(s) 249, 1143, 1361, 1668
BstF5I GGATG 2 cut(s) 1602, 2533
BstH2I RGCGCY 1 cut(s) 1261
BstHHI GCGC 4 cut(s) 1029, 1260, 1995, 2026
BstKTI GATC 5 cut(s) 280, 561, 1972, 2434, 2765
BstMAI GTCTC 8 cut(s) 503, 665, 667, 1074, 1502, 1835, 2115, 2166
BstMBI GATC 5 cut(s) 277, 558, 1969, 2431, 2762
BstMCI CGRYCG 1 cut(s) 2434
BstMWI GCNNNNNNNGC 7 cut(s) 74, 922, 1051, 2030, 2059, 2774, 3007
BstNI CCWGG 8 cut(s) 374, 538, 909, 1262, 1464, 1974, 2692, 2808
BstNSI RCATGY 6 cut(s) 1033, 1045, 1363, 1682, 1856, 2069
BstPAI GACNNNNGTC 1 cut(s) 1446
BstSCI CCNGG 8 cut(s) 372, 536, 907, 1260, 1462, 1972, 2690, 2806
BstSFI CTRYAG 1 cut(s) 2976
BstSLI GKGCMC 2 cut(s) 10, 45
BstV1I GCAGC 9 cut(s) 693, 1036, 1194, 1289, 2065, 2277, 2474, 2641, 2990
BstV2I GAAGAC 2 cut(s) 302, 1554
BstX2I RGATCY 1 cut(s) 277
BstXI CCANNNNNNTGG 1 cut(s) 1452
BstYI RGATCY 1 cut(s) 277
Bsu15I ATCGAT 2 cut(s) 1968, 2072
Bsu36I CCTNAGG 1 cut(s) 2160
BsuRI GGCC 4 cut(s) 1587, 1844, 2758, 2800
BsuTUI ATCGAT 2 cut(s) 1968, 2072
BtgZI GCGATG 1 cut(s) 1106
BtsCI GGATG 2 cut(s) 1602, 2533
BtsI GCAGTG 2 cut(s) 691, 2801
BtsIMutI CAGTG 4 cut(s) 211, 626, 691, 2801
BveI ACCTGC 2 cut(s) 294, 1935
Cac8I GCNNGC 4 cut(s) 249, 1143, 1361, 1668
CaiI CAGNNNCTG 4 cut(s) 919, 2003, 2059, 2123
CfoI GCGC 4 cut(s) 1029, 1260, 1995, 2026
Cfr13I GGNCC 4 cut(s) 1585, 1924, 2156, 2756
ClaI ATCGAT 2 cut(s) 1968, 2072
CseI GACGC 1 cut(s) 2911
Csp6I GTAC 9 cut(s) 108, 842, 871, 1520, 1762, 2133, 2335, 2393, 2872
CviQI GTAC 9 cut(s) 108, 842, 871, 1520, 1762, 2133, 2335, 2393, 2872
DpnI GATC 5 cut(s) 279, 560, 1971, 2433, 2764
DpnII GATC 5 cut(s) 277, 558, 1969, 2431, 2762
DraI TTTAAA 2 cut(s) 1287, 2815
EaeI YGGCCR 1 cut(s) 2798
Eam1104I CTCTTC 5 cut(s) 1155, 1758, 2510, 2557, 3010
EarI CTCTTC 5 cut(s) 1155, 1758, 2510, 2557, 3010
Eco130I CCWWGG 2 cut(s) 1722, 1824
Eco31I GGTCTC 1 cut(s) 1835
Eco32I GATATC 2 cut(s) 1194, 1966
Eco47I GGWCC 2 cut(s) 1924, 2156
Eco57I CTGAAG 2 cut(s) 2589, 2664
Eco81I CCTNAGG 1 cut(s) 2160
EcoO109I RGGNCCY 2 cut(s) 2156, 2756
EcoRI GAATTC 1 cut(s) 640
EcoRII CCWGG 8 cut(s) 372, 536, 907, 1260, 1462, 1972, 2690, 2806
EcoRV GATATC 2 cut(s) 1194, 1966
EcoT14I CCWWGG 2 cut(s) 1722, 1824
EcoT22I ATGCAT 2 cut(s) 1858, 2071
ErhI CCWWGG 2 cut(s) 1722, 1824
Esp3I CGTCTC 1 cut(s) 1074
FalI AAGNNNNNCTT 6 cut(s) 170, 202, 1817, 1849, 2795, 2827
FaqI GGGAC 2 cut(s) 1058, 1117
FauI CCCGC 1 cut(s) 682
FokI GGATG 2 cut(s) 1609, 2540
FspBI CTAG 8 cut(s) 426, 1058, 1142, 1211, 1907, 2651, 2703, 2946
FspI TGCGCA 1 cut(s) 2025
GlaI GCGC 4 cut(s) 1028, 1259, 1994, 2025
GsuI CTGGAG 7 cut(s) 470, 559, 1446, 1859, 2033, 2439, 2688
HaeII RGCGCY 1 cut(s) 1261
HaeIII GGCC 4 cut(s) 1587, 1844, 2758, 2800
HapII CCGG 3 cut(s) 321, 503, 2919
HgaI GACGC 1 cut(s) 2911
HhaI GCGC 4 cut(s) 1029, 1260, 1995, 2026
Hin1I GRCGYC 1 cut(s) 2922
Hin6I GCGC 4 cut(s) 1027, 1258, 1993, 2024
HinP1I GCGC 4 cut(s) 1027, 1258, 1993, 2024
HincII GTYRAC 4 cut(s) 1151, 1347, 1983, 2415
HindII GTYRAC 4 cut(s) 1151, 1347, 1983, 2415
HindIII AAGCTT 3 cut(s) 66, 245, 1802
HpaI GTTAAC 2 cut(s) 1347, 1983
HpaII CCGG 3 cut(s) 321, 503, 2919
HphI GGTGA 5 cut(s) 608, 986, 1286, 2378, 2555
Hpy166II GTNNAC 9 cut(s) 474, 700, 964, 1151, 1347, 1764, 1983, 2415, 2474
Hpy188I TCNGA 8 cut(s) 670, 868, 1618, 1861, 2091, 2119, 2436, 2968
Hpy188III TCNNGA 9 cut(s) 132, 219, 1211, 1872, 2225, 2276, 2479, 2835, 2996
Hpy8I GTNNAC 9 cut(s) 474, 700, 964, 1151, 1347, 1764, 1983, 2415, 2474
Hpy99I CGWCG 2 cut(s) 2472, 2987
HpyAV CCTTC 7 cut(s) 88, 965, 971, 1014, 1331, 1728, 2688
HpyCH4IV ACGT 1 cut(s) 1083
HpyF10VI GCNNNNNNNGC 7 cut(s) 74, 922, 1051, 2030, 2059, 2774, 3007
HpySE526I ACGT 1 cut(s) 1083
Hsp92I GRCGYC 1 cut(s) 2922
HspAI GCGC 4 cut(s) 1027, 1258, 1993, 2024
KpnI GGTACC 1 cut(s) 2136
KspAI GTTAAC 2 cut(s) 1347, 1983
Kzo9I GATC 5 cut(s) 277, 558, 1969, 2431, 2762
LmnI GCTCC 7 cut(s) 903, 1255, 1465, 1640, 2305, 2383, 2662
Lsp1109I GCAGC 9 cut(s) 693, 1036, 1194, 1289, 2065, 2277, 2474, 2641, 2990
LweI GCATC 9 cut(s) 1128, 1723, 1757, 1865, 1951, 2065, 2078, 2518, 2932
MaeI CTAG 8 cut(s) 426, 1058, 1142, 1211, 1907, 2651, 2703, 2946
MaeII ACGT 1 cut(s) 1083
MalI GATC 5 cut(s) 279, 560, 1971, 2433, 2764
MboI GATC 5 cut(s) 277, 558, 1969, 2431, 2762
MfeI CAATTG 1 cut(s) 2265
MflI RGATCY 1 cut(s) 277
MhlI GDGCHC 4 cut(s) 10, 45, 163, 900
MlsI TGGCCA 1 cut(s) 2800
MluNI TGGCCA 1 cut(s) 2800
MlyI GAGTC 6 cut(s) 469, 823, 1139, 1805, 1907, 2266
MmeI TCCRAC 2 cut(s) 1546, 1902
Mox20I TGGCCA 1 cut(s) 2800
Mph1103I ATGCAT 2 cut(s) 1858, 2071
MroXI GAANNNNTTC 1 cut(s) 2373
MscI TGGCCA 1 cut(s) 2800
MslI CAYNNNNRTG 3 cut(s) 38, 122, 1231
Msp20I TGGCCA 1 cut(s) 2800
MspI CCGG 3 cut(s) 321, 503, 2919
MspR9I CCNGG 8 cut(s) 374, 538, 909, 1262, 1464, 1974, 2692, 2808
MunI CAATTG 1 cut(s) 2265
Mva1269I GAATGC 2 cut(s) 117, 2936
MvaI CCWGG 8 cut(s) 374, 538, 909, 1262, 1464, 1974, 2692, 2808
MwoI GCNNNNNNNGC 7 cut(s) 74, 922, 1051, 2030, 2059, 2774, 3007
NdeII GATC 5 cut(s) 277, 558, 1969, 2431, 2762
NheI GCTAGC 1 cut(s) 1141
NlaIV GGNNCC 4 cut(s) 7, 415, 2134, 2149
NmuCI GTSAC 3 cut(s) 614, 1060, 2269
NsbI TGCGCA 1 cut(s) 2025
NsiI ATGCAT 2 cut(s) 1858, 2071
NspI RCATGY 6 cut(s) 1033, 1045, 1363, 1682, 1856, 2069
NspV TTCGAA 1 cut(s) 293
PaeI GCATGC 1 cut(s) 1363
PcsI WCGNNNNNNNCGW 2 cut(s) 1766, 1974
PctI GAATGC 2 cut(s) 117, 2936
PdmI GAANNNNTTC 1 cut(s) 2373
PflMI CCANNNNNTGG 1 cut(s) 2666
PfoI TCCNGGA 2 cut(s) 372, 536
Ple19I CGATCG 1 cut(s) 2434
PleI GAGTC 6 cut(s) 469, 822, 1138, 1804, 1907, 2266
PpsI GAGTC 6 cut(s) 469, 822, 1138, 1804, 1907, 2266
PpuMI RGGWCCY 1 cut(s) 2156
PshAI GACNNNNGTC 1 cut(s) 1446
PshBI ATTAAT 1 cut(s) 1167
Psp5II RGGWCCY 1 cut(s) 2156
Psp6I CCWGG 8 cut(s) 372, 536, 907, 1260, 1462, 1972, 2690, 2806
PspGI CCWGG 8 cut(s) 372, 536, 907, 1260, 1462, 1972, 2690, 2806
PspN4I GGNNCC 4 cut(s) 7, 415, 2134, 2149
PspPI GGNCC 4 cut(s) 1585, 1924, 2156, 2756
PspPPI RGGWCCY 1 cut(s) 2156
PstI CTGCAG 1 cut(s) 2980
PstNI CAGNNNCTG 4 cut(s) 919, 2003, 2059, 2123
PsuI RGATCY 1 cut(s) 277
PvuI CGATCG 1 cut(s) 2434
RsaI GTAC 9 cut(s) 109, 843, 872, 1521, 1763, 2134, 2336, 2394, 2873
RsaNI GTAC 9 cut(s) 108, 842, 871, 1520, 1762, 2133, 2335, 2393, 2872
RseI CAYNNNNRTG 3 cut(s) 38, 122, 1231
Sau3AI GATC 5 cut(s) 277, 558, 1969, 2431, 2762
Sau96I GGNCC 4 cut(s) 1585, 1924, 2156, 2756
ScaI AGTACT 2 cut(s) 872, 2873
SchI GAGTC 6 cut(s) 469, 823, 1139, 1805, 1907, 2266
ScrFI CCNGG 8 cut(s) 374, 538, 909, 1262, 1464, 1974, 2692, 2808
SduI GDGCHC 4 cut(s) 10, 45, 163, 900
SfaNI GCATC 9 cut(s) 1128, 1723, 1757, 1865, 1951, 2065, 2078, 2518, 2932
SfcI CTRYAG 1 cut(s) 2976
SfuI TTCGAA 1 cut(s) 293
SinI GGWCC 2 cut(s) 1924, 2156
SmiMI CAYNNNNRTG 3 cut(s) 38, 122, 1231
SmlI CTYRAG 3 cut(s) 2285, 2477, 2486
SmoI CTYRAG 3 cut(s) 2285, 2477, 2486
SphI GCATGC 1 cut(s) 1363
SsiI CCGC 4 cut(s) 689, 947, 2381, 2718
SspI AATATT 1 cut(s) 199
SspMI CTAG 8 cut(s) 426, 1058, 1142, 1211, 1907, 2651, 2703, 2946
StyD4I CCNGG 8 cut(s) 372, 536, 907, 1260, 1462, 1972, 2690, 2806
StyI CCWWGG 2 cut(s) 1722, 1824
TaiI ACGT 1 cut(s) 1086
TaqI TCGA 6 cut(s) 293, 1695, 1769, 1968, 2072, 2761
TatI WGTACW 3 cut(s) 870, 2392, 2871
TauI GCSGC 1 cut(s) 2720
TscAI CASTG 4 cut(s) 211, 626, 691, 2801
TseFI GTSAC 3 cut(s) 614, 1060, 2269
TseI GCWGC 9 cut(s) 681, 1024, 1182, 1302, 2053, 2290, 2462, 2654, 2978
Tsp45I GTSAC 3 cut(s) 614, 1060, 2269
TspGWI ACGGA 1 cut(s) 1415
TspRI CASTG 4 cut(s) 211, 626, 691, 2801
Van91I CCANNNNNTGG 1 cut(s) 2666
VpaK11BI GGWCC 2 cut(s) 1924, 2156
VspI ATTAAT 1 cut(s) 1167
XapI RAATTY 6 cut(s) 377, 640, 1535, 1814, 1901, 2241
XbaI TCTAGA 1 cut(s) 1210
XceI RCATGY 6 cut(s) 1033, 1045, 1363, 1682, 1856, 2069
XmnI GAANNNNTTC 1 cut(s) 2373
XspI CTAG 8 cut(s) 426, 1058, 1142, 1211, 1907, 2651, 2703, 2946
ZrmI AGTACT 2 cut(s) 872, 2873
Zsp2I ATGCAT 2 cut(s) 1858, 2071
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.