pycom08g06670

NADH dehydrogenase ubiquinone iron-sulfur protein 4, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0002607
Physical Location & Seq
Reverse (-)
5212556 .. 5214840
2285 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 636 bp
ATGTTCCCAACTCCCGAAAGAAAAATAAAAAATAAAAAATGTTGTTTCCACCAAACACTCGTACTTACACGGTTGGGCTTTTCGTCACGGGAACCATTGTTGCGCTTTCGCCTGAGGCAACCTCGAAGCTTTTCGCTCCCACCAAAAATGGCCCGATCTCTACGACAGGGCTTCAACATCGCCCGAGCGCACGTGCTATCGCGGCCGCGATGGGCGTCGTCGCAGTCTTCGGAGGTGCTGGTGGAGGTCAATGCCCCCGACTTTGGCATGATCTCCGGAATCCCCGAACAGCATCTCCGGCGAAGGGTTATAATCTATTCGCCTGCTCGAACTGCGAGCCAACAAGGTTCTGGGAAAGTCGGGAGATGGAAAATTAATTTTCTTTCAACGCAGAAGTGGGAAAACCCATTGATGGGATGGACATCTACAGGTGATCCCTACGCCAATGTTGGTGAAGCAGGACTGAGTTTTGAGAGTGAAGAAGCTGCAAAGGCATTTGCTGAGAAACATGGCTGGGATTATACGGTCAGGAAGCGTCACACCCCACTTTTGAAGGTCAAGGCTTATGCAGACAACTTCAAGTGGAAGGGCCCTGAAAAGAGTGAGAAGACTGCAGCTGATGCTGCGAACATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko04723 ko04932 ko05010 ko05012 ko05016 map00190 map01100 map04714 map04723 map04932 map05010 map05012 map05016
Pfam Domains
Protein Families
ETC_C1_NDUFA4

Protein Analysis

212

Amino Acids

23.99

Weight (kDa)

10.36

Isoelectric Point (pI)

57.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NDUS4 PF04800 103 - 196 5.2e-31 NADH dehydrogenase ubiquinone Fe-S protein 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 311
AccII CGCG 2 cut(s) 202, 208
AccIII TCCGGA 1 cut(s) 275
AciI CCGC 2 cut(s) 202, 206
AclWI GGATC 1 cut(s) 428
AcoI YGGCCR 1 cut(s) 203
AcvI CACGTG 1 cut(s) 193
AcyI GRCGYC 1 cut(s) 215
AfaI GTAC 1 cut(s) 63
AfiI CCNNNNNNNGG 4 cut(s) 263, 304, 412, 413
AgsI TTSAA 4 cut(s) 175, 387, 553, 580
AloI GAACNNNNNNTCC 2 cut(s) 279, 311
AluBI AGCT 3 cut(s) 129, 485, 617
AluI AGCT 3 cut(s) 129, 485, 617
AlwI GGATC 1 cut(s) 428
Ama87I CYCGRG 1 cut(s) 183
Aor13HI TCCGGA 1 cut(s) 275
AoxI GGCC 3 cut(s) 150, 203, 589
ApaI GGGCCC 1 cut(s) 593
ApeKI GCWGC 3 cut(s) 485, 614, 623
AseI ATTAAT 1 cut(s) 375
Asp700I GAANNNNTTC 1 cut(s) 130
AspLEI GCGC 2 cut(s) 105, 190
AspS9I GGNCC 3 cut(s) 151, 589, 590
AsuHPI GGTGA 2 cut(s) 443, 464
AvaI CYCGRG 1 cut(s) 183
AxyI CCTNAGG 1 cut(s) 113
BaeGI GKGCMC 1 cut(s) 593
BanII GRGCYC 1 cut(s) 593
BbrPI CACGTG 1 cut(s) 193
BbsI GAAGAC 2 cut(s) 219, 614
BbvI GCAGC 3 cut(s) 472, 610, 626
BccI CCATC 4 cut(s) 204, 360, 406, 411
BfmI CTRYAG 2 cut(s) 426, 612
BisI GCNGC 5 cut(s) 203, 206, 486, 615, 624
BlsI GCNGC 5 cut(s) 204, 207, 487, 616, 625
BmeT110I CYCGRG 1 cut(s) 183
BmgT120I GGNCC 3 cut(s) 151, 589, 590
BmiI GGNNCC 2 cut(s) 93, 591
BmsI GCATC 2 cut(s) 301, 610
BpiI GAAGAC 2 cut(s) 219, 614
BplI GAGNNNNNCTC 2 cut(s) 106, 138
BsaAI YACGTR 1 cut(s) 193
BsaBI GATNNNNATC 1 cut(s) 421
BsaHI GRCGYC 1 cut(s) 215
BsaWI WCCGGW 1 cut(s) 275
BsaXI ACNNNNNCTCC 2 cut(s) 279, 309
Bsc4I CCNNNNNNNGG 4 cut(s) 263, 304, 412, 413
Bse21I CCTNAGG 1 cut(s) 113
Bse8I GATNNNNATC 1 cut(s) 421
BseAI TCCGGA 1 cut(s) 275
BseGI GGATG 1 cut(s) 422
BseJI GATNNNNATC 1 cut(s) 421
BseLI CCNNNNNNNGG 4 cut(s) 263, 304, 412, 413
BseMII CTCAG 3 cut(s) 104, 455, 492
BseSI GKGCMC 1 cut(s) 593
BseX3I CGGCCG 1 cut(s) 203
BseXI GCAGC 3 cut(s) 472, 610, 626
BseYI CCCAGC 1 cut(s) 513
Bsh1236I CGCG 2 cut(s) 202, 208
Bsh1285I CGRYCG 1 cut(s) 206
BshFI GGCC 3 cut(s) 152, 205, 591
BsiEI CGRYCG 1 cut(s) 206
BsiHKCI CYCGRG 1 cut(s) 183
BsiSI CCGG 2 cut(s) 276, 298
BslI CCNNNNNNNGG 4 cut(s) 263, 304, 412, 413
BsnI GGCC 3 cut(s) 152, 205, 591
BsoBI CYCGRG 1 cut(s) 183
Bsp120I GGGCCC 1 cut(s) 589
Bsp1286I GDGCHC 1 cut(s) 593
Bsp13I TCCGGA 1 cut(s) 275
Bsp143I GATC 3 cut(s) 155, 270, 433
BspACI CCGC 2 cut(s) 202, 206
BspANI GGCC 3 cut(s) 152, 205, 591
BspCNI CTCAG 3 cut(s) 105, 456, 493
BspEI TCCGGA 1 cut(s) 275
BspFNI CGCG 2 cut(s) 202, 208
BspLI GGNNCC 2 cut(s) 93, 591
BspMAI CTGCAG 1 cut(s) 616
BspPI GGATC 1 cut(s) 428
BssMI GATC 3 cut(s) 155, 270, 433
BssNI GRCGYC 1 cut(s) 215
Bst4CI ACNGT 2 cut(s) 72, 526
BstACI GRCGYC 1 cut(s) 215
BstAPI GCANNNNNTGC 1 cut(s) 620
BstBAI YACGTR 1 cut(s) 193
BstC8I GCNNGC 2 cut(s) 324, 337
BstDEI CTNAG 3 cut(s) 113, 464, 501
BstF5I GGATG 1 cut(s) 422
BstFNI CGCG 2 cut(s) 202, 208
BstHHI GCGC 2 cut(s) 105, 190
BstKTI GATC 3 cut(s) 158, 273, 436
BstMBI GATC 3 cut(s) 155, 270, 433
BstMCI CGRYCG 1 cut(s) 206
BstMWI GCNNNNNNNGC 6 cut(s) 202, 298, 332, 491, 620, 623
BstSFI CTRYAG 2 cut(s) 426, 612
BstSLI GKGCMC 1 cut(s) 593
BstUI CGCG 2 cut(s) 202, 208
BstV1I GCAGC 3 cut(s) 472, 610, 626
BstV2I GAAGAC 2 cut(s) 219, 614
BstZI CGGCCG 1 cut(s) 203
Bsu36I CCTNAGG 1 cut(s) 113
BsuRI GGCC 3 cut(s) 152, 205, 591
BtgZI GCGATG 2 cut(s) 163, 223
BtsCI GGATG 1 cut(s) 422
Cac8I GCNNGC 2 cut(s) 324, 337
CciNI GCGGCCGC 1 cut(s) 203
CfoI GCGC 2 cut(s) 105, 190
Cfr13I GGNCC 3 cut(s) 151, 589, 590
CseI GACGC 2 cut(s) 204, 524
Csp6I GTAC 1 cut(s) 62
CviAII CATG 2 cut(s) 268, 509
CviQI GTAC 1 cut(s) 62
DdeI CTNAG 3 cut(s) 113, 464, 501
DpnI GATC 3 cut(s) 157, 272, 435
DpnII GATC 3 cut(s) 155, 270, 433
EaeI YGGCCR 1 cut(s) 203
EagI CGGCCG 1 cut(s) 203
EclXI CGGCCG 1 cut(s) 203
Eco24I GRGCYC 1 cut(s) 593
Eco52I CGGCCG 1 cut(s) 203
Eco72I CACGTG 1 cut(s) 193
Eco81I CCTNAGG 1 cut(s) 113
Eco88I CYCGRG 1 cut(s) 183
EcoO109I RGGNCCY 2 cut(s) 589, 590
EcoT38I GRGCYC 1 cut(s) 593
FaeI CATG 2 cut(s) 271, 512
FaiI YATR 5 cut(s) 269, 311, 510, 522, 567
FatI CATG 2 cut(s) 267, 508
Fnu4HI GCNGC 5 cut(s) 203, 206, 486, 615, 624
FokI GGATG 1 cut(s) 429
FriOI GRGCYC 1 cut(s) 593
Fsp4HI GCNGC 5 cut(s) 203, 206, 486, 615, 624
GlaI GCGC 2 cut(s) 104, 189
GluI GCNGC 5 cut(s) 203, 206, 486, 615, 624
GsaI CCCAGC 1 cut(s) 517
HaeIII GGCC 3 cut(s) 152, 205, 591
HapII CCGG 2 cut(s) 276, 298
HgaI GACGC 2 cut(s) 204, 524
HhaI GCGC 2 cut(s) 105, 190
Hin1I GRCGYC 1 cut(s) 215
Hin1II CATG 2 cut(s) 271, 512
Hin6I GCGC 2 cut(s) 103, 188
HinP1I GCGC 2 cut(s) 103, 188
HindIII AAGCTT 1 cut(s) 127
HinfI GANTC 1 cut(s) 279
HpaII CCGG 2 cut(s) 276, 298
HphI GGTGA 2 cut(s) 443, 464
Hpy188I TCNGA 2 cut(s) 232, 635
Hpy188III TCNNGA 4 cut(s) 14, 276, 361, 529
Hpy99I CGWCG 2 cut(s) 220, 223
HpyAV CCTTC 3 cut(s) 297, 547, 580
HpyCH4III ACNGT 2 cut(s) 72, 526
HpyCH4IV ACGT 1 cut(s) 192
HpyCH4V TGCA 3 cut(s) 488, 569, 614
HpyF10VI GCNNNNNNNGC 6 cut(s) 202, 298, 332, 491, 620, 623
HpyF3I CTNAG 3 cut(s) 113, 464, 501
HpySE526I ACGT 1 cut(s) 192
Hsp92I GRCGYC 1 cut(s) 215
Hsp92II CATG 2 cut(s) 271, 512
HspAI GCGC 2 cut(s) 103, 188
Kpn2I TCCGGA 1 cut(s) 275
Kzo9I GATC 3 cut(s) 155, 270, 433
LmnI GCTCC 1 cut(s) 141
Lsp1109I GCAGC 3 cut(s) 472, 610, 626
LweI GCATC 2 cut(s) 301, 610
MaeII ACGT 1 cut(s) 192
MaeIII GTNAC 2 cut(s) 84, 536
MalI GATC 3 cut(s) 157, 272, 435
MboI GATC 3 cut(s) 155, 270, 433
MboII GAAGA 3 cut(s) 219, 491, 619
MhlI GDGCHC 1 cut(s) 593
MluCI AATT 2 cut(s) 372, 376
MnlI CCTC 4 cut(s) 108, 132, 226, 238
MroI TCCGGA 1 cut(s) 275
MroXI GAANNNNTTC 1 cut(s) 130
MseI TTAA 1 cut(s) 375
MspA1I CMGCKG 1 cut(s) 617
MspI CCGG 2 cut(s) 276, 298
MvnI CGCG 2 cut(s) 202, 208
MwoI GCNNNNNNNGC 6 cut(s) 202, 298, 332, 491, 620, 623
NdeII GATC 3 cut(s) 155, 270, 433
NlaIII CATG 2 cut(s) 271, 512
NlaIV GGNNCC 2 cut(s) 93, 591
NmuCI GTSAC 2 cut(s) 84, 536
NotI GCGGCCGC 1 cut(s) 203
PdmI GAANNNNTTC 1 cut(s) 130
PfeI GAWTC 1 cut(s) 279
PkrI GCNGC 5 cut(s) 204, 207, 487, 616, 625
PmaCI CACGTG 1 cut(s) 193
PmlI CACGTG 1 cut(s) 193
Ppu21I YACGTR 1 cut(s) 193
PshBI ATTAAT 1 cut(s) 375
PsiI TTATAA 1 cut(s) 311
PspCI CACGTG 1 cut(s) 193
PspFI CCCAGC 1 cut(s) 513
PspN4I GGNNCC 2 cut(s) 93, 591
PspOMI GGGCCC 1 cut(s) 589
PspPI GGNCC 3 cut(s) 151, 589, 590
PstI CTGCAG 1 cut(s) 616
PvuII CAGCTG 1 cut(s) 617
RsaI GTAC 1 cut(s) 63
RsaNI GTAC 1 cut(s) 62
SaqAI TTAA 1 cut(s) 375
SatI GCNGC 5 cut(s) 203, 206, 486, 615, 624
Sau3AI GATC 3 cut(s) 155, 270, 433
Sau96I GGNCC 3 cut(s) 151, 589, 590
SduI GDGCHC 1 cut(s) 593
SfaNI GCATC 2 cut(s) 301, 610
SfcI CTRYAG 2 cut(s) 426, 612
Sse9I AATT 2 cut(s) 372, 376
SsiI CCGC 2 cut(s) 202, 206
TaaI ACNGT 2 cut(s) 72, 526
TaiI ACGT 1 cut(s) 195
TaqI TCGA 2 cut(s) 124, 328
TasI AATT 2 cut(s) 372, 376
TauI GCSGC 2 cut(s) 205, 208
TfiI GAWTC 1 cut(s) 279
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TseFI GTSAC 2 cut(s) 84, 536
TseI GCWGC 3 cut(s) 485, 614, 623
Tsp45I GTSAC 2 cut(s) 84, 536
VspI ATTAAT 1 cut(s) 375
XcmI CCANNNNNNNNNTGG 2 cut(s) 347, 414
XmnI GAANNNNTTC 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.