pycom08g12610

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0004715
Physical Location & Seq
Reverse (-)
11085028 .. 11087937
2910 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1284 bp
ATGAATGGGACCTTCAGCGAGAATGCTATGACGGAGACTGCAGCATCAAGTGGGAAAATTGGAAATGCTGATAAGGTGGATGACGGTGGGACTAATAGCTCATCTGTTTGGGAAGCTATAGAGGCCAATGTCAACCCTGAAAGTAATGGTTTGACTGTTGCTTTGACTATTGAAGATGAAGTGAAAGGTTCTACTCATTCAAAGCCCGCCAAAGTGCATAAAGGTCCAGGTAAGAGCAAGAATGAGAAGGCCCCTGGTCCCAAGAGTGTCTCCCCAACTTTGATGAAGAAAAGCAAAGATGGAAATGGCACAGAGGCAAAAGCTTCTGTTTCAAATGGTTCAGCTGCTTCAAATTCACGCCCGAAACAGCCTAACAAGAATAGATCATTTAATGGGAGACAGGTTCAGTCATCCAATCAACCTGAAAAACTTGATGCAGAATTGTCTGAAGGCAGTGTGGAGAAGGCGAAGCTGAAGCCTTTGAAAAAAGGCTCCCAGAATCAGGCTGAAGGAGAATCACAATCCTCTTTAAGTCCAACAGAAGGAGATAAACAGCCTAGGGTTGGGACACTTCCGAAATATGGTTTCAGTTTTAGGTGCGATGAACGAGCTGAGAAAAGGAGAGAGTTCTATACTAAACTGGAGGAAAAGATACATGCAAAGGAGATGGAGAAGAATAATTTGCAAGCCAAGTCCGAGGAAACTCTGGAAGCTGAGATTAGGATGCTCAGGAAGAAACTGACTTTTAAAGCAACTCCAATGCCAAGCTTCTATCAGGAGCCTCCACCTCCTAAAGTGGAGCTGAAGAAGATACCAACTACAAGAGCCAAGTCTCCCAAGCTCGGACGTAGGAAGAGCTTACCTCCTGCAGTTTCTGAAGCACACGGTAATACCAATGGTCGATCTAGCAGGCTTAGTCTGGATGAGAAAGTACCTCAGAACACTGCTAAAGGGCCCTCTCCTGTGCATCCAAAGAAGCCACAACGGAAGTCTCTTCCTAGACTCCCGTCTGAGAAGACCACTTTACCAAATGTGGTGAATGAGCCAAAAACGACTTCTAAGGCAGTAAATGAGGAAAAGACCAACTTAATTGCTGCAATGATTGAGGAGAATGCCACCGTGCCCACTGCAGAAAGTGAAGCTGGATCTCACACCCAAGAGGAGGAGGCAGCAGTTCCCAAGGCCGAGGTAAGCAAAGAGCAGTCCCATACAGATGACGAGACAGCGGATGAAGACCAACACCAGCCCGTCTATGTTCAAGAACCTATAACCTCGGAGCATTGA

Protein Analysis

428

Amino Acids

46.4

Weight (kDa)

8.96

Isoelectric Point (pI)

51.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 196 - 271 4.3e-27 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 207, 1226
AclWI GGATC 1 cut(s) 1153
AcsI RAATTY 1 cut(s) 352
AcuI CTGAAG 5 cut(s) 468, 494, 528, 824, 897
AfaI GTAC 1 cut(s) 933
AfiI CCNNNNNNNGG 6 cut(s) 502, 542, 563, 581, 842, 1162
AgsI TTSAA 6 cut(s) 173, 201, 333, 351, 484, 1259
AjnI CCWGG 2 cut(s) 226, 253
AjuI GAANNNNNNNTTGG 2 cut(s) 1039, 1071
Alw26I GTCTC 6 cut(s) 29, 274, 391, 837, 996, 1214
AlwI GGATC 1 cut(s) 1153
AlwNI CAGNNNCTG 1 cut(s) 875
AoxI GGCC 4 cut(s) 123, 249, 953, 1182
ApaI GGGCCC 1 cut(s) 957
ApeKI GCWGC 4 cut(s) 41, 344, 1094, 1169
ApoI RAATTY 1 cut(s) 352
ArsI GACNNNNNNTTYG 2 cut(s) 145, 177
AspA2I CCTAGG 1 cut(s) 557
AspS9I GGNCC 6 cut(s) 9, 224, 250, 257, 953, 954
AsuHPI GGTGA 1 cut(s) 1048
AvaII GGWCC 3 cut(s) 9, 224, 257
AvrII CCTAGG 1 cut(s) 557
BaeGI GKGCMC 2 cut(s) 957, 1125
BanII GRGCYC 1 cut(s) 957
BbsI GAAGAC 2 cut(s) 1022, 1239
BbvI GCAGC 4 cut(s) 53, 331, 1081, 1181
BccI CCATC 2 cut(s) 293, 661
BciT130I CCWGG 2 cut(s) 228, 255
BcoDI GTCTC 6 cut(s) 29, 274, 391, 837, 996, 1214
BfaI CTAG 3 cut(s) 558, 906, 999
BfmI CTRYAG 4 cut(s) 39, 117, 867, 1128
BisI GCNGC 4 cut(s) 42, 345, 1095, 1170
BlnI CCTAGG 1 cut(s) 557
BlsI GCNGC 4 cut(s) 43, 346, 1096, 1171
Bme1390I CCNGG 2 cut(s) 228, 255
Bme18I GGWCC 3 cut(s) 9, 224, 257
BmgT120I GGNCC 6 cut(s) 9, 224, 250, 257, 953, 954
BmiI GGNNCC 6 cut(s) 10, 252, 259, 493, 780, 955
BmrFI CCNGG 2 cut(s) 228, 255
BmsI GCATC 4 cut(s) 53, 424, 714, 976
BoxI GACNNNNGTC 1 cut(s) 1006
BpiI GAAGAC 2 cut(s) 1022, 1239
BplI GAGNNNNNCTC 2 cut(s) 847, 879
BpmI CTGGAG 1 cut(s) 662
Bpu10I CCTNAGC 1 cut(s) 728
BsaJI CCNNGG 6 cut(s) 253, 557, 696, 1179, 1185, 1272
Bsc4I CCNNNNNNNGG 6 cut(s) 502, 542, 563, 581, 842, 1162
Bse1I ACTGG 1 cut(s) 645
Bse3DI GCAATG 1 cut(s) 1104
BseBI CCWGG 2 cut(s) 228, 255
BseDI CCNNGG 6 cut(s) 253, 557, 696, 1179, 1185, 1272
BseGI GGATG 6 cut(s) 85, 410, 729, 928, 967, 1234
BseLI CCNNNNNNNGG 6 cut(s) 502, 542, 563, 581, 842, 1162
BseMI GCAATG 1 cut(s) 1104
BseMII CTCAG 5 cut(s) 603, 705, 742, 950, 1002
BseNI ACTGG 1 cut(s) 645
BseRI GAGGAG 3 cut(s) 1121, 1175, 1178
BseSI GKGCMC 2 cut(s) 957, 1125
BseXI GCAGC 4 cut(s) 53, 331, 1081, 1181
BshFI GGCC 4 cut(s) 125, 251, 955, 1184
BslFI GGGAC 5 cut(s) 22, 103, 243, 580, 1189
BslI CCNNNNNNNGG 6 cut(s) 502, 542, 563, 581, 842, 1162
BsmAI GTCTC 6 cut(s) 29, 274, 391, 837, 996, 1214
BsmFI GGGAC 5 cut(s) 22, 103, 243, 580, 1189
BsmI GAATGC 2 cut(s) 28, 1117
BsnI GGCC 4 cut(s) 125, 251, 955, 1184
Bsp120I GGGCCC 1 cut(s) 953
Bsp1286I GDGCHC 2 cut(s) 957, 1125
Bsp143I GATC 3 cut(s) 383, 902, 1145
BspACI CCGC 2 cut(s) 207, 1226
BspANI GGCC 4 cut(s) 125, 251, 955, 1184
BspCNI CTCAG 5 cut(s) 604, 706, 741, 949, 1003
BspLI GGNNCC 6 cut(s) 10, 252, 259, 493, 780, 955
BspMAI CTGCAG 3 cut(s) 43, 871, 1132
BspPI GGATC 1 cut(s) 1153
BspQI GCTCTTC 1 cut(s) 848
BsrDI GCAATG 1 cut(s) 1104
BsrI ACTGG 1 cut(s) 645
BssECI CCNNGG 6 cut(s) 253, 557, 696, 1179, 1185, 1272
BssMI GATC 3 cut(s) 383, 902, 1145
BssT1I CCWWGG 2 cut(s) 557, 1179
Bst2UI CCWGG 2 cut(s) 228, 255
Bst4CI ACNGT 4 cut(s) 86, 157, 887, 1120
Bst6I CTCTTC 2 cut(s) 848, 999
BstC8I GCNNGC 3 cut(s) 207, 687, 911
BstDEI CTNAG 7 cut(s) 612, 714, 728, 914, 936, 1011, 1059
BstF5I GGATG 6 cut(s) 85, 410, 729, 928, 967, 1234
BstKTI GATC 3 cut(s) 386, 905, 1148
BstMAI GTCTC 6 cut(s) 29, 274, 391, 837, 996, 1214
BstMBI GATC 3 cut(s) 383, 902, 1145
BstMWI GCNNNNNNNGC 1 cut(s) 122
BstNI CCWGG 2 cut(s) 228, 255
BstNSI RCATGY 1 cut(s) 659
BstPAI GACNNNNGTC 1 cut(s) 1006
BstSCI CCNGG 2 cut(s) 226, 253
BstSFI CTRYAG 4 cut(s) 39, 117, 867, 1128
BstSLI GKGCMC 2 cut(s) 957, 1125
BstV1I GCAGC 4 cut(s) 53, 331, 1081, 1181
BstV2I GAAGAC 2 cut(s) 1022, 1239
BstX2I RGATCY 1 cut(s) 1145
BstYI RGATCY 1 cut(s) 1145
BsuRI GGCC 4 cut(s) 125, 251, 955, 1184
BtgZI GCGATG 1 cut(s) 615
BtsCI GGATG 6 cut(s) 85, 410, 729, 928, 967, 1234
BtsI GCAGTG 3 cut(s) 460, 942, 1125
BtsIMutI CAGTG 3 cut(s) 460, 942, 1125
Cac8I GCNNGC 3 cut(s) 207, 687, 911
CaiI CAGNNNCTG 1 cut(s) 875
Cfr13I GGNCC 6 cut(s) 9, 224, 250, 257, 953, 954
Csp6I GTAC 1 cut(s) 932
CviAII CATG 1 cut(s) 656
CviQI GTAC 1 cut(s) 932
DdeI CTNAG 7 cut(s) 612, 714, 728, 914, 936, 1011, 1059
DpnI GATC 3 cut(s) 385, 904, 1147
DpnII GATC 3 cut(s) 383, 902, 1145
DraI TTTAAA 1 cut(s) 748
Eam1104I CTCTTC 2 cut(s) 848, 999
EarI CTCTTC 2 cut(s) 848, 999
Eco130I CCWWGG 2 cut(s) 557, 1179
Eco24I GRGCYC 1 cut(s) 957
Eco47I GGWCC 3 cut(s) 9, 224, 257
Eco57I CTGAAG 5 cut(s) 468, 494, 528, 824, 897
EcoO109I RGGNCCY 4 cut(s) 9, 250, 953, 954
EcoRII CCWGG 2 cut(s) 226, 253
EcoT14I CCWWGG 2 cut(s) 557, 1179
EcoT38I GRGCYC 1 cut(s) 957
ErhI CCWWGG 2 cut(s) 557, 1179
FaeI CATG 1 cut(s) 659
FaiI YATR 9 cut(s) 29, 119, 219, 582, 633, 657, 1209, 1254, 1268
FalI AAGNNNNNCTT 2 cut(s) 1070, 1102
FaqI GGGAC 5 cut(s) 22, 103, 243, 580, 1189
FatI CATG 1 cut(s) 655
FauI CCCGC 1 cut(s) 214
Fnu4HI GCNGC 4 cut(s) 42, 345, 1095, 1170
FokI GGATG 6 cut(s) 92, 397, 736, 935, 954, 1241
FriOI GRGCYC 1 cut(s) 957
Fsp4HI GCNGC 4 cut(s) 42, 345, 1095, 1170
FspBI CTAG 3 cut(s) 558, 906, 999
GluI GCNGC 4 cut(s) 42, 345, 1095, 1170
GsuI CTGGAG 1 cut(s) 662
HaeIII GGCC 4 cut(s) 125, 251, 955, 1184
Hin1II CATG 1 cut(s) 659
HincII GTYRAC 1 cut(s) 133
HindII GTYRAC 1 cut(s) 133
HindIII AAGCTT 2 cut(s) 321, 766
HinfI GANTC 3 cut(s) 499, 515, 1002
HphI GGTGA 1 cut(s) 1048
Hpy166II GTNNAC 1 cut(s) 133
Hpy188I TCNGA 8 cut(s) 448, 576, 697, 845, 877, 939, 1012, 1276
Hpy188III TCNNGA 5 cut(s) 707, 730, 776, 920, 1259
Hpy8I GTNNAC 1 cut(s) 133
HpyAV CCTTC 6 cut(s) 22, 241, 443, 457, 503, 536
HpyCH4III ACNGT 4 cut(s) 86, 157, 887, 1120
HpyCH4IV ACGT 1 cut(s) 847
HpyCH4V TGCA 9 cut(s) 41, 217, 437, 659, 685, 869, 967, 1097, 1130
HpyF10VI GCNNNNNNNGC 1 cut(s) 122
HpyF3I CTNAG 7 cut(s) 612, 714, 728, 914, 936, 1011, 1059
HpySE526I ACGT 1 cut(s) 847
Hsp92II CATG 1 cut(s) 659
Kzo9I GATC 3 cut(s) 383, 902, 1145
LguI GCTCTTC 1 cut(s) 848
LmnI GCTCC 4 cut(s) 497, 778, 799, 1276
Lsp1109I GCAGC 4 cut(s) 53, 331, 1081, 1181
LweI GCATC 4 cut(s) 53, 424, 714, 976
MaeI CTAG 3 cut(s) 558, 906, 999
MaeII ACGT 1 cut(s) 847
MalI GATC 3 cut(s) 385, 904, 1147
MboI GATC 3 cut(s) 383, 902, 1145
MflI RGATCY 1 cut(s) 1145
MhlI GDGCHC 2 cut(s) 957, 1125
MluCI AATT 5 cut(s) 57, 352, 440, 679, 1089
MlyI GAGTC 1 cut(s) 996
MmeI TCCRAC 1 cut(s) 560
MseI TTAA 4 cut(s) 390, 530, 747, 1088
MslI CAYNNNNRTG 1 cut(s) 1212
MspA1I CMGCKG 2 cut(s) 344, 1226
MspR9I CCNGG 2 cut(s) 228, 255
Mva1269I GAATGC 2 cut(s) 28, 1117
MvaI CCWGG 2 cut(s) 228, 255
MwoI GCNNNNNNNGC 1 cut(s) 122
NdeII GATC 3 cut(s) 383, 902, 1145
NlaIII CATG 1 cut(s) 659
NlaIV GGNNCC 6 cut(s) 10, 252, 259, 493, 780, 955
NmeAIII GCCGAG 1 cut(s) 1210
NspI RCATGY 1 cut(s) 659
PciSI GCTCTTC 1 cut(s) 848
PctI GAATGC 2 cut(s) 28, 1117
PfeI GAWTC 2 cut(s) 499, 515
PkrI GCNGC 4 cut(s) 43, 346, 1096, 1171
PleI GAGTC 1 cut(s) 996
PpsI GAGTC 1 cut(s) 996
PpuMI RGGWCCY 1 cut(s) 9
PshAI GACNNNNGTC 1 cut(s) 1006
Psp5II RGGWCCY 1 cut(s) 9
Psp6I CCWGG 2 cut(s) 226, 253
PspGI CCWGG 2 cut(s) 226, 253
PspN4I GGNNCC 6 cut(s) 10, 252, 259, 493, 780, 955
PspOMI GGGCCC 1 cut(s) 953
PspPI GGNCC 6 cut(s) 9, 224, 250, 257, 953, 954
PspPPI RGGWCCY 1 cut(s) 9
PstI CTGCAG 3 cut(s) 43, 871, 1132
PstNI CAGNNNCTG 1 cut(s) 875
PsuI RGATCY 1 cut(s) 1145
PvuII CAGCTG 1 cut(s) 344
RsaI GTAC 1 cut(s) 933
RsaNI GTAC 1 cut(s) 932
RseI CAYNNNNRTG 1 cut(s) 1212
SapI GCTCTTC 1 cut(s) 848
SaqAI TTAA 4 cut(s) 390, 530, 747, 1088
SatI GCNGC 4 cut(s) 42, 345, 1095, 1170
Sau3AI GATC 3 cut(s) 383, 902, 1145
Sau96I GGNCC 6 cut(s) 9, 224, 250, 257, 953, 954
SchI GAGTC 1 cut(s) 996
ScrFI CCNGG 2 cut(s) 228, 255
SduI GDGCHC 2 cut(s) 957, 1125
SfaNI GCATC 4 cut(s) 53, 424, 714, 976
SfcI CTRYAG 4 cut(s) 39, 117, 867, 1128
SinI GGWCC 3 cut(s) 9, 224, 257
SmiMI CAYNNNNRTG 1 cut(s) 1212
Sse9I AATT 5 cut(s) 57, 352, 440, 679, 1089
SsiI CCGC 2 cut(s) 207, 1226
SspMI CTAG 3 cut(s) 558, 906, 999
StyD4I CCNGG 2 cut(s) 226, 253
StyI CCWWGG 2 cut(s) 557, 1179
TaaI ACNGT 4 cut(s) 86, 157, 887, 1120
TaiI ACGT 1 cut(s) 850
TaqI TCGA 1 cut(s) 901
TasI AATT 5 cut(s) 57, 352, 440, 679, 1089
TfiI GAWTC 2 cut(s) 499, 515
Tru1I TTAA 4 cut(s) 390, 530, 747, 1088
Tru9I TTAA 4 cut(s) 390, 530, 747, 1088
TscAI CASTG 3 cut(s) 460, 949, 1132
TseI GCWGC 4 cut(s) 41, 344, 1094, 1169
TspDTI ATGAA 5 cut(s) 17, 192, 299, 618, 1245
TspGWI ACGGA 2 cut(s) 47, 1000
TspRI CASTG 3 cut(s) 460, 949, 1132
VpaK11BI GGWCC 3 cut(s) 9, 224, 257
XapI RAATTY 1 cut(s) 352
XceI RCATGY 1 cut(s) 659
XmaJI CCTAGG 1 cut(s) 557
XspI CTAG 3 cut(s) 558, 906, 999
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.