pycom09g18970

Cysteine-rich receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0005518
Physical Location & Seq
Reverse (-)
19751269 .. 19752011
743 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g18970.1

Sequence Viewer

Length: 687 bp
ATGGAATCTCAACACAGATCACAGACCACTACATTCACTGCAGCTGCTGGGTCACTGCTAGTAGATCTTCAGCTTGCAACACCCAAAATCAACGGTTTCTTTGCAGCAACTAAGAACGAAGTTGTTAGTAGTGGAGGTAATGGAAATGCAACCGTCTACGGCGTGGCACAATGCGCCGAGACAATTAGCAAGATCGGTTGCCAAGATTGCTTGAATGTGGCATACAACAACGTACAGAGCTGCCTTCCAGATACCGATGGTAGGGCAGTTGACGCAGGTTGTTTCATGCGGTACTCTAGTACTTCTTTCTTCTCTGATAACCAGACTACGAACATCGCGGTTGTCAAATTTGTTCACCAAATGACATCTCAGCGCTCATTCCTTAATCGAACAAACTCGATAATGCTAACAATACTTGTAGGAGGTAATTCAAGAAAGAAAGGCATAATCATTGGAGTGGCAGTTGGAGGTGAAGGTCTTATTCTTCTTCTCATTGTTGGAATTCTTCTCTATTTTAAACTCTCAAGAAAGTCCACGGCGGATCATAGAGGCGACATATTGGGAGCAACGGAGTTGCAAGGACCAGTGAATTACAAATATAAAGATTTGAAATCAGCAACAAAAATTTTTAGTGAAGAAAACAAACTAGGAGAAGGAGGTTTCGGAGACGTTTACAAGGTAAAATAA

Protein Analysis

229

Amino Acids

24.44

Weight (kDa)

8.93

Isoelectric Point (pI)

26.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 6 - 100 1.1e-12 Salt stress response/antifungal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 266
AccI GTMKAC 1 cut(s) 156
AccII CGCG 1 cut(s) 338
AciI CCGC 3 cut(s) 289, 338, 539
AclWI GGATC 1 cut(s) 549
AcsI RAATTY 3 cut(s) 347, 501, 624
AcuI CTGAAG 1 cut(s) 53
AfaI GTAC 3 cut(s) 234, 293, 301
AfeI AGCGCT 1 cut(s) 374
AfiI CCNNNNNNNGG 1 cut(s) 261
AgsI TTSAA 3 cut(s) 214, 432, 610
AluBI AGCT 3 cut(s) 44, 73, 240
AluI AGCT 3 cut(s) 44, 73, 240
Alw26I GTCTC 2 cut(s) 173, 660
AlwI GGATC 1 cut(s) 549
AlwNI CAGNNNCTG 1 cut(s) 47
Aor51HI AGCGCT 1 cut(s) 374
ApeKI GCWGC 4 cut(s) 41, 44, 104, 240
ApoI RAATTY 3 cut(s) 347, 501, 624
AspLEI GCGC 2 cut(s) 176, 375
AspS9I GGNCC 1 cut(s) 581
AsuHPI GGTGA 2 cut(s) 347, 482
AvaII GGWCC 1 cut(s) 581
BaeI ACNNNNGTAYC 2 cut(s) 283, 316
BbvI GCAGC 4 cut(s) 31, 53, 116, 227
BccI CCATC 1 cut(s) 251
BceAI ACGGC 2 cut(s) 175, 552
BcoDI GTCTC 2 cut(s) 173, 660
BfaI CTAG 3 cut(s) 59, 297, 647
BfmI CTRYAG 1 cut(s) 39
BfoI RGCGCY 1 cut(s) 376
BfuAI ACCTGC 1 cut(s) 266
BglII AGATCT 1 cut(s) 64
BisI GCNGC 4 cut(s) 42, 45, 105, 241
BlsI GCNGC 4 cut(s) 43, 46, 106, 242
BmcAI AGTACT 1 cut(s) 301
Bme18I GGWCC 1 cut(s) 581
BmgT120I GGNCC 1 cut(s) 581
BpuEI CTTGAG 1 cut(s) 508
BsaJI CCNNGG 1 cut(s) 534
BsaXI ACNNNNNCTCC 2 cut(s) 447, 477
Bsc4I CCNNNNNNNGG 1 cut(s) 261
Bse1I ACTGG 1 cut(s) 584
BseDI CCNNGG 1 cut(s) 534
BseLI CCNNNNNNNGG 1 cut(s) 261
BseMII CTCAG 1 cut(s) 383
BseNI ACTGG 1 cut(s) 584
BseXI GCAGC 4 cut(s) 31, 53, 116, 227
BseYI CCCAGC 1 cut(s) 47
Bsh1236I CGCG 1 cut(s) 338
BslI CCNNNNNNNGG 1 cut(s) 261
BsmAI GTCTC 2 cut(s) 173, 660
BsmBI CGTCTC 1 cut(s) 660
Bsp143I GATC 4 cut(s) 17, 64, 192, 541
BspACI CCGC 3 cut(s) 289, 338, 539
BspCNI CTCAG 1 cut(s) 382
BspFNI CGCG 1 cut(s) 338
BspMAI CTGCAG 1 cut(s) 43
BspMI ACCTGC 1 cut(s) 266
BspPI GGATC 1 cut(s) 549
BsrI ACTGG 1 cut(s) 584
BssECI CCNNGG 1 cut(s) 534
BssMI GATC 4 cut(s) 17, 64, 192, 541
Bst4CI ACNGT 2 cut(s) 95, 154
BstC8I GCNNGC 1 cut(s) 75
BstDEI CTNAG 2 cut(s) 111, 369
BstDSI CCRYGG 1 cut(s) 534
BstFNI CGCG 1 cut(s) 338
BstH2I RGCGCY 1 cut(s) 376
BstHHI GCGC 2 cut(s) 176, 375
BstKTI GATC 4 cut(s) 20, 67, 195, 544
BstMAI GTCTC 2 cut(s) 173, 660
BstMBI GATC 4 cut(s) 17, 64, 192, 541
BstMWI GCNNNNNNNGC 3 cut(s) 173, 207, 272
BstSFI CTRYAG 1 cut(s) 39
BstUI CGCG 1 cut(s) 338
BstV1I GCAGC 4 cut(s) 31, 53, 116, 227
BstX2I RGATCY 1 cut(s) 64
BstYI RGATCY 1 cut(s) 64
BtgI CCRYGG 1 cut(s) 534
BtgZI GCGATG 1 cut(s) 319
BtsI GCAGTG 2 cut(s) 36, 53
BtsIMutI CAGTG 3 cut(s) 36, 53, 591
BveI ACCTGC 1 cut(s) 266
Cac8I GCNNGC 1 cut(s) 75
CaiI CAGNNNCTG 1 cut(s) 47
CfoI GCGC 2 cut(s) 176, 375
Cfr13I GGNCC 1 cut(s) 581
CseI GACGC 1 cut(s) 281
Csp6I GTAC 3 cut(s) 233, 292, 300
CviAII CATG 1 cut(s) 286
CviJI RGCY 3 cut(s) 44, 73, 240
CviKI_1 RGCY 3 cut(s) 44, 73, 240
CviQI GTAC 3 cut(s) 233, 292, 300
DdeI CTNAG 2 cut(s) 111, 369
DpnI GATC 4 cut(s) 19, 66, 194, 543
DpnII GATC 4 cut(s) 17, 64, 192, 541
DraI TTTAAA 1 cut(s) 517
EciI GGCGGA 1 cut(s) 554
Eco47I GGWCC 1 cut(s) 581
Eco47III AGCGCT 1 cut(s) 374
Eco57I CTGAAG 1 cut(s) 53
EcoRI GAATTC 1 cut(s) 501
Esp3I CGTCTC 1 cut(s) 660
FaeI CATG 1 cut(s) 289
FaiI YATR 6 cut(s) 223, 287, 446, 546, 557, 600
FatI CATG 1 cut(s) 285
FblI GTMKAC 1 cut(s) 156
Fnu4HI GCNGC 4 cut(s) 42, 45, 105, 241
Fsp4HI GCNGC 4 cut(s) 42, 45, 105, 241
FspBI CTAG 3 cut(s) 59, 297, 647
GlaI GCGC 2 cut(s) 175, 374
GluI GCNGC 4 cut(s) 42, 45, 105, 241
GsaI CCCAGC 1 cut(s) 51
HaeII RGCGCY 1 cut(s) 376
HgaI GACGC 1 cut(s) 281
HhaI GCGC 2 cut(s) 176, 375
Hin1II CATG 1 cut(s) 289
Hin6I GCGC 2 cut(s) 174, 373
HinP1I GCGC 2 cut(s) 174, 373
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HinfI GANTC 1 cut(s) 5
HphI GGTGA 2 cut(s) 347, 482
Hpy166II GTNNAC 5 cut(s) 157, 271, 355, 534, 673
Hpy188I TCNGA 2 cut(s) 316, 665
Hpy188III TCNNGA 3 cut(s) 248, 432, 525
Hpy8I GTNNAC 5 cut(s) 157, 271, 355, 534, 673
HpyAV CCTTC 3 cut(s) 254, 467, 647
HpyCH4III ACNGT 2 cut(s) 95, 154
HpyCH4IV ACGT 2 cut(s) 231, 669
HpyCH4V TGCA 5 cut(s) 41, 77, 104, 149, 577
HpyF10VI GCNNNNNNNGC 3 cut(s) 173, 207, 272
HpyF3I CTNAG 2 cut(s) 111, 369
HpySE526I ACGT 2 cut(s) 231, 669
Hsp92II CATG 1 cut(s) 289
HspAI GCGC 2 cut(s) 174, 373
Kzo9I GATC 4 cut(s) 17, 64, 192, 541
LmnI GCTCC 1 cut(s) 563
LpnPI CCDG 5 cut(s) 33, 261, 261, 335, 597
Lsp1109I GCAGC 4 cut(s) 31, 53, 116, 227
MaeI CTAG 3 cut(s) 59, 297, 647
MaeII ACGT 2 cut(s) 231, 669
MaeIII GTNAC 1 cut(s) 51
MalI GATC 4 cut(s) 19, 66, 194, 543
MboI GATC 4 cut(s) 17, 64, 192, 541
MboII GAAGA 6 cut(s) 59, 301, 476, 479, 497, 647
MflI RGATCY 1 cut(s) 64
MluCI AATT 6 cut(s) 183, 347, 427, 501, 589, 624
MmeI TCCRAC 2 cut(s) 445, 478
MnlI CCTC 5 cut(s) 128, 416, 461, 542, 650
MseI TTAA 2 cut(s) 384, 516
MslI CAYNNNNRTG 1 cut(s) 455
MspA1I CMGCKG 1 cut(s) 44
MvnI CGCG 1 cut(s) 338
MwoI GCNNNNNNNGC 3 cut(s) 173, 207, 272
NdeII GATC 4 cut(s) 17, 64, 192, 541
NlaIII CATG 1 cut(s) 289
NmeAIII GCCGAG 1 cut(s) 202
NmuCI GTSAC 1 cut(s) 51
PfeI GAWTC 1 cut(s) 5
PkrI GCNGC 4 cut(s) 43, 46, 106, 242
PspFI CCCAGC 1 cut(s) 47
PspPI GGNCC 1 cut(s) 581
PstI CTGCAG 1 cut(s) 43
PstNI CAGNNNCTG 1 cut(s) 47
PsuI RGATCY 1 cut(s) 64
PvuII CAGCTG 1 cut(s) 44
RsaI GTAC 3 cut(s) 234, 293, 301
RsaNI GTAC 3 cut(s) 233, 292, 300
RseI CAYNNNNRTG 1 cut(s) 455
SaqAI TTAA 2 cut(s) 384, 516
SatI GCNGC 4 cut(s) 42, 45, 105, 241
Sau3AI GATC 4 cut(s) 17, 64, 192, 541
Sau96I GGNCC 1 cut(s) 581
ScaI AGTACT 1 cut(s) 301
SfcI CTRYAG 1 cut(s) 39
SinI GGWCC 1 cut(s) 581
SmiMI CAYNNNNRTG 1 cut(s) 455
SmlI CTYRAG 1 cut(s) 523
SmoI CTYRAG 1 cut(s) 523
Sse9I AATT 6 cut(s) 183, 347, 427, 501, 589, 624
SsiI CCGC 3 cut(s) 289, 338, 539
SspMI CTAG 3 cut(s) 59, 297, 647
TaaI ACNGT 2 cut(s) 95, 154
TaiI ACGT 2 cut(s) 234, 672
TaqI TCGA 2 cut(s) 388, 398
TasI AATT 6 cut(s) 183, 347, 427, 501, 589, 624
TatI WGTACW 1 cut(s) 299
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 2 cut(s) 384, 516
Tru9I TTAA 2 cut(s) 384, 516
TscAI CASTG 3 cut(s) 43, 60, 591
TseFI GTSAC 1 cut(s) 51
TseI GCWGC 4 cut(s) 41, 44, 104, 240
Tsp45I GTSAC 1 cut(s) 51
TspDTI ATGAA 1 cut(s) 274
TspGWI ACGGA 1 cut(s) 584
TspRI CASTG 3 cut(s) 43, 60, 591
VpaK11BI GGWCC 1 cut(s) 581
XapI RAATTY 3 cut(s) 347, 501, 624
XmiI GTMKAC 1 cut(s) 156
XspI CTAG 3 cut(s) 59, 297, 647
ZrmI AGTACT 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.