Rroxscaffold_5G00352940

Cysteine-rich receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005518
Physical Location & Seq
Forward (+)
30345298 .. 30353459
8162 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00352940.1

Sequence Viewer

Length: 1170 bp
ATGATGAATAAGAAGATATTGACAACACTGTCAATAATTTCGATCAGCCAGATATGGTGGTGGGGTTGTGTGAGTGCAGAACCGCAGATCAGTCTGCTGAACGAGGGTTGCAGCCAGTACAACGCAACCAGCTTGTCCAACTTCTATATCAATCTCAACGCCACTTTCTTGGACCTCAAAGCGCAACTACTCAACGGCAGCAAACACCTTGCAACGGCGCAACAGTTCAAGGGCTCCGACCCCGTCCACGCCATGGCGCAGTGTAGGAACTACCTCTCCTCCGCCGACTGTCTATCTTGCTTTGCCGCCGCCGTCTCCCAAATCCGCAACTGCTCTGCCTCCAATAGCGCACGCGTCGTCTATGACGGCTGCTTCCTCCGGTATGAGAGCAGTGCTACCCTTCCTGGTAATGTAGGACTCTGTGGGAATCAAACTGGATCGCCTGCTACAACCTTTACTGCAGCTGCTGAAGAACTTCTAATGGATCTTCAAGTTGCTACGCCCAAGATTAATGGCTTCTTTGCAGCATCTAAGGAGAAAAGCAGCTTCCAAGAGCCGTACTTGAAATCAAGAGCCTGGATGTGTTCTGATCATCATTGTGAGCACCCTTACTTTTGTCTACCTCCAAACTGTGCAAACTTTAATTATTATTCAAATTGCATGAATGAGTTCATAAACATTAGCTCTAGATCAAAATCAGGTAACGAATTTATGCTGCTGCATTGTGTTGTCGGGGAGCCGGTTAATCTCCGGCCGCCTATTGAAAGCTGCTTGGCACACCTCCGTCTCGCTCCGGCGTCGAAGTTAACCAGTCCACAACAGGCAAGGCGGCGACTTAAGAAGAAAGCAACCTCCACAACAGGGATCATCCTCACTCTCTCCATTTTACGACCGCCGGCTTTGCTCTCTTCGGGCTTCGGACTTGAGGTTCCGAGCTCGAGGCTCCGGTTCTTTCGCGAAGCCGACCTCAAAACCCTATGTCCGGAATCACCGAAACCCATTGATTCGGGGTTCTCCGCCACCAACCCGACCAAGTCCCCCTTCCATCCGTTCAGTACGGTAACTCAAGGTCGAACAGTTTCAAATTGTGAGAGCTCGGCCACGGAGACGACGGCGTATAGAGGTGGGTCGAATCGGACCGGTGGAACCTTGCACCGGATGATCCGCTAA

Protein Analysis

389

Amino Acids

42.56

Weight (kDa)

9.0

Isoelectric Point (pI)

47.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 37 - 130 5.1e-15 Salt stress response/antifungal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 28
AccB7I CCANNNNNTGG 1 cut(s) 253
AccI GTMKAC 1 cut(s) 619
AccII CGCG 2 cut(s) 354, 957
AccIII TCCGGA 1 cut(s) 982
AclWI GGATC 4 cut(s) 445, 492, 872, 1156
AcoI YGGCCR 2 cut(s) 752, 1098
AcsI RAATTY 1 cut(s) 707
AcuI CTGAAG 1 cut(s) 489
AcyI GRCGYC 1 cut(s) 797
AfaI GTAC 3 cut(s) 119, 560, 1057
AfiI CCNNNNNNNGG 5 cut(s) 214, 253, 861, 982, 1155
AflII CTTAAG 1 cut(s) 836
AflIII ACRYGT 1 cut(s) 352
AgeI ACCGGT 1 cut(s) 1139
AgsI TTSAA 6 cut(s) 229, 491, 565, 654, 764, 1083
AjnI CCWGG 2 cut(s) 403, 575
AjuI GAANNNNNNNTTGG 2 cut(s) 1025, 1057
AloI GAACNNNNNNTCC 4 cut(s) 260, 292, 912, 944
AluBI AGCT 7 cut(s) 132, 464, 546, 684, 768, 936, 1095
AluI AGCT 7 cut(s) 132, 464, 546, 684, 768, 936, 1095
Alw21I GWGCWC 3 cut(s) 606, 938, 1097
Alw26I GTCTC 3 cut(s) 319, 791, 1100
AlwI GGATC 4 cut(s) 445, 492, 872, 1156
AlwNI CAGNNNCTG 1 cut(s) 467
Ama87I CYCGRG 1 cut(s) 937
Aor13HI TCCGGA 1 cut(s) 982
AoxI GGCC 2 cut(s) 752, 1098
ApoI RAATTY 1 cut(s) 707
AseI ATTAAT 1 cut(s) 510
AsiGI ACCGGT 1 cut(s) 1139
Asp700I GAANNNNTTC 3 cut(s) 474, 668, 1078
AspLEI GCGC 4 cut(s) 184, 220, 259, 350
AspS9I GGNCC 2 cut(s) 172, 1137
AsuHPI GGTGA 1 cut(s) 981
AvaI CYCGRG 1 cut(s) 937
AvaII GGWCC 2 cut(s) 172, 1137
BanII GRGCYC 3 cut(s) 236, 938, 1097
Bbv12I GWGCWC 3 cut(s) 606, 938, 1097
BccI CCATC 1 cut(s) 1053
BceAI ACGGC 6 cut(s) 211, 231, 296, 382, 541, 1128
BciT130I CCWGG 2 cut(s) 405, 577
BclI TGATCA 1 cut(s) 589
BcoDI GTCTC 3 cut(s) 319, 791, 1100
BfaI CTAG 1 cut(s) 687
BfmI CTRYAG 1 cut(s) 459
BfrI CTTAAG 1 cut(s) 836
Bme1390I CCNGG 2 cut(s) 405, 577
Bme18I GGWCC 2 cut(s) 172, 1137
BmeT110I CYCGRG 1 cut(s) 937
BmgT120I GGNCC 2 cut(s) 172, 1137
BmiI GGNNCC 5 cut(s) 235, 738, 930, 944, 1147
BmrFI CCNGG 2 cut(s) 405, 577
BmsI GCATC 1 cut(s) 536
BpuEI CTTGAG 2 cut(s) 944, 1050
BsaBI GATNNNNATC 1 cut(s) 694
BsaHI GRCGYC 1 cut(s) 797
BsaJI CCNNGG 2 cut(s) 252, 1101
BsaWI WCCGGW 5 cut(s) 378, 945, 982, 1139, 1155
BsaXI ACNNNNNCTCC 4 cut(s) 260, 263, 290, 293
Bsc4I CCNNNNNNNGG 5 cut(s) 214, 253, 861, 982, 1155
Bse118I RCCGGY 3 cut(s) 739, 895, 1139
Bse1I ACTGG 3 cut(s) 115, 439, 810
Bse8I GATNNNNATC 1 cut(s) 694
BseAI TCCGGA 1 cut(s) 982
BseBI CCWGG 2 cut(s) 405, 577
BseDI CCNNGG 2 cut(s) 252, 1101
BseGI GGATG 4 cut(s) 585, 867, 1045, 1164
BseJI GATNNNNATC 1 cut(s) 694
BseLI CCNNNNNNNGG 5 cut(s) 214, 253, 861, 982, 1155
BseNI ACTGG 3 cut(s) 115, 439, 810
BseRI GAGGAG 1 cut(s) 268
BseX3I CGGCCG 1 cut(s) 752
BsgI GTGCAG 1 cut(s) 96
Bsh1236I CGCG 2 cut(s) 354, 957
Bsh1285I CGRYCG 2 cut(s) 755, 893
BshFI GGCC 2 cut(s) 754, 1100
BshTI ACCGGT 1 cut(s) 1139
BsiEI CGRYCG 2 cut(s) 755, 893
BsiHKAI GWGCWC 3 cut(s) 606, 938, 1097
BsiHKCI CYCGRG 1 cut(s) 937
BsiSI CCGG 9 cut(s) 379, 740, 751, 794, 896, 946, 983, 1140, 1156
BslFI GGGAC 1 cut(s) 1021
BslI CCNNNNNNNGG 5 cut(s) 214, 253, 861, 982, 1155
BsmAI GTCTC 3 cut(s) 319, 791, 1100
BsmBI CGTCTC 3 cut(s) 319, 791, 1100
BsmFI GGGAC 1 cut(s) 1021
BsnI GGCC 2 cut(s) 754, 1100
BsoBI CYCGRG 1 cut(s) 937
Bsp1286I GDGCHC 4 cut(s) 236, 606, 938, 1097
Bsp13I TCCGGA 1 cut(s) 982
Bsp143I GATC 8 cut(s) 42, 87, 437, 484, 589, 689, 864, 1161
Bsp19I CCATGG 1 cut(s) 252
Bsp68I TCGCGA 1 cut(s) 957
BspANI GGCC 2 cut(s) 754, 1100
BspEI TCCGGA 1 cut(s) 982
BspFNI CGCG 2 cut(s) 354, 957
BspLI GGNNCC 5 cut(s) 235, 738, 930, 944, 1147
BspMAI CTGCAG 1 cut(s) 463
BspPI GGATC 4 cut(s) 445, 492, 872, 1156
BspTI CTTAAG 1 cut(s) 836
BsrFI RCCGGY 3 cut(s) 739, 895, 1139
BsrI ACTGG 3 cut(s) 115, 439, 810
BssAI RCCGGY 3 cut(s) 739, 895, 1139
BssECI CCNNGG 2 cut(s) 252, 1101
BssMI GATC 8 cut(s) 42, 87, 437, 484, 589, 689, 864, 1161
BssNI GRCGYC 1 cut(s) 797
BssT1I CCWWGG 1 cut(s) 252
Bst2UI CCWGG 2 cut(s) 405, 577
Bst4CI ACNGT 6 cut(s) 30, 225, 290, 632, 1060, 1078
Bst6I CTCTTC 1 cut(s) 913
BstACI GRCGYC 1 cut(s) 797
BstAFI CTTAAG 1 cut(s) 836
BstC8I GCNNGC 3 cut(s) 352, 444, 897
BstDEI CTNAG 1 cut(s) 531
BstDSI CCRYGG 2 cut(s) 252, 1101
BstF5I GGATG 4 cut(s) 585, 867, 1045, 1164
BstFNI CGCG 2 cut(s) 354, 957
BstHHI GCGC 4 cut(s) 184, 220, 259, 350
BstKTI GATC 8 cut(s) 45, 90, 440, 487, 592, 692, 867, 1164
BstMAI GTCTC 3 cut(s) 319, 791, 1100
BstMBI GATC 8 cut(s) 42, 87, 437, 484, 589, 689, 864, 1161
BstMCI CGRYCG 2 cut(s) 755, 893
BstMWI GCNNNNNNNGC 1 cut(s) 901
BstNI CCWGG 2 cut(s) 405, 577
BstSCI CCNGG 2 cut(s) 403, 575
BstSFI CTRYAG 1 cut(s) 459
BstUI CGCG 2 cut(s) 354, 957
BstX2I RGATCY 1 cut(s) 484
BstXI CCANNNNNNTGG 1 cut(s) 169
BstYI RGATCY 1 cut(s) 484
BstZI CGGCCG 1 cut(s) 752
BsuRI GGCC 2 cut(s) 754, 1100
BtgI CCRYGG 2 cut(s) 252, 1101
BtsCI GGATG 4 cut(s) 585, 867, 1045, 1164
BtsI GCAGTG 2 cut(s) 266, 397
BtsIMutI CAGTG 3 cut(s) 26, 266, 397
BtuMI TCGCGA 1 cut(s) 957
Cac8I GCNNGC 3 cut(s) 352, 444, 897
CaiI CAGNNNCTG 1 cut(s) 467
CfoI GCGC 4 cut(s) 184, 220, 259, 350
Cfr10I RCCGGY 3 cut(s) 739, 895, 1139
Cfr13I GGNCC 2 cut(s) 172, 1137
CpoI CGGWCCG 1 cut(s) 1137
CseI GACGC 2 cut(s) 343, 786
Csp6I GTAC 3 cut(s) 118, 559, 1056
CspAI ACCGGT 1 cut(s) 1139
CspI CGGWCCG 1 cut(s) 1137
CviAII CATG 2 cut(s) 253, 661
CviQI GTAC 3 cut(s) 118, 559, 1056
DdeI CTNAG 1 cut(s) 531
DpnI GATC 8 cut(s) 44, 89, 439, 486, 591, 691, 866, 1163
DpnII GATC 8 cut(s) 42, 87, 437, 484, 589, 689, 864, 1161
DrdI GACNNNNNNGTC 1 cut(s) 28
DseDI GACNNNNNNGTC 1 cut(s) 28
EaeI YGGCCR 2 cut(s) 752, 1098
EagI CGGCCG 1 cut(s) 752
Eam1104I CTCTTC 1 cut(s) 913
EarI CTCTTC 1 cut(s) 913
EciI GGCGGA 2 cut(s) 271, 1006
Ecl136II GAGCTC 2 cut(s) 936, 1095
EclXI CGGCCG 1 cut(s) 752
Eco130I CCWWGG 1 cut(s) 252
Eco24I GRGCYC 3 cut(s) 236, 938, 1097
Eco47I GGWCC 2 cut(s) 172, 1137
Eco52I CGGCCG 1 cut(s) 752
Eco53kI GAGCTC 2 cut(s) 936, 1095
Eco57I CTGAAG 1 cut(s) 489
Eco88I CYCGRG 1 cut(s) 937
EcoICRI GAGCTC 2 cut(s) 936, 1095
EcoRII CCWGG 2 cut(s) 403, 575
EcoT14I CCWWGG 1 cut(s) 252
EcoT38I GRGCYC 3 cut(s) 236, 938, 1097
ErhI CCWWGG 1 cut(s) 252
Esp3I CGTCTC 3 cut(s) 319, 791, 1100
FaeI CATG 2 cut(s) 256, 664
FalI AAGNNNNNCTT 2 cut(s) 1025, 1057
FaqI GGGAC 1 cut(s) 1021
FatI CATG 2 cut(s) 252, 660
FbaI TGATCA 1 cut(s) 589
FblI GTMKAC 1 cut(s) 619
FokI GGATG 3 cut(s) 592, 854, 1032
FriOI GRGCYC 3 cut(s) 236, 938, 1097
FspBI CTAG 1 cut(s) 687
GlaI GCGC 4 cut(s) 183, 219, 258, 349
HaeIII GGCC 2 cut(s) 754, 1100
HapII CCGG 9 cut(s) 379, 740, 751, 794, 896, 946, 983, 1140, 1156
HgaI GACGC 2 cut(s) 343, 786
HhaI GCGC 4 cut(s) 184, 220, 259, 350
Hin1I GRCGYC 1 cut(s) 797
Hin1II CATG 2 cut(s) 256, 664
Hin6I GCGC 4 cut(s) 182, 218, 257, 348
HinP1I GCGC 4 cut(s) 182, 218, 257, 348
HincII GTYRAC 1 cut(s) 807
HindII GTYRAC 1 cut(s) 807
HinfI GANTC 5 cut(s) 417, 427, 986, 1004, 1132
HpaI GTTAAC 1 cut(s) 807
HpaII CCGG 9 cut(s) 379, 740, 751, 794, 896, 946, 983, 1140, 1156
HphI GGTGA 1 cut(s) 981
Hpy166II GTNNAC 4 cut(s) 247, 620, 807, 815
Hpy188I TCNGA 5 cut(s) 238, 589, 920, 933, 1137
Hpy188III TCNNGA 4 cut(s) 570, 687, 956, 983
Hpy8I GTNNAC 4 cut(s) 247, 620, 807, 815
Hpy99I CGWCG 3 cut(s) 359, 802, 1114
HpyAV CCTTC 2 cut(s) 410, 1051
HpyCH4III ACNGT 6 cut(s) 30, 225, 290, 632, 1060, 1078
HpyCH4V TGCA 9 cut(s) 77, 111, 212, 461, 524, 635, 660, 721, 1153
HpyF10VI GCNNNNNNNGC 1 cut(s) 901
HpyF3I CTNAG 1 cut(s) 531
Hsp92I GRCGYC 1 cut(s) 797
Hsp92II CATG 2 cut(s) 256, 664
HspAI GCGC 4 cut(s) 182, 218, 257, 348
Kpn2I TCCGGA 1 cut(s) 982
KroI GCCGGC 1 cut(s) 895
KroNI GCCGGC 1 cut(s) 897
Ksp22I TGATCA 1 cut(s) 589
KspAI GTTAAC 1 cut(s) 807
Kzo9I GATC 8 cut(s) 42, 87, 437, 484, 589, 689, 864, 1161
LmnI GCTCC 4 cut(s) 239, 736, 796, 948
LweI GCATC 1 cut(s) 536
MaeI CTAG 1 cut(s) 687
MaeIII GTNAC 2 cut(s) 701, 1060
MalI GATC 8 cut(s) 44, 89, 439, 486, 591, 691, 866, 1163
MboI GATC 8 cut(s) 42, 87, 437, 484, 589, 689, 864, 1161
MboII GAAGA 5 cut(s) 25, 479, 482, 853, 900
MflI RGATCY 1 cut(s) 484
MhlI GDGCHC 4 cut(s) 236, 606, 938, 1097
MluCI AATT 5 cut(s) 36, 643, 655, 707, 1084
MluI ACGCGT 1 cut(s) 352
MlyI GAGTC 1 cut(s) 411
MmeI TCCRAC 2 cut(s) 162, 261
MroI TCCGGA 1 cut(s) 982
MroNI GCCGGC 1 cut(s) 895
MroXI GAANNNNTTC 3 cut(s) 474, 668, 1078
MseI TTAA 5 cut(s) 510, 642, 744, 806, 837
MslI CAYNNNNRTG 1 cut(s) 597
MspA1I CMGCKG 1 cut(s) 464
MspCI CTTAAG 1 cut(s) 836
MspI CCGG 9 cut(s) 379, 740, 751, 794, 896, 946, 983, 1140, 1156
MspR9I CCNGG 2 cut(s) 405, 577
MvaI CCWGG 2 cut(s) 405, 577
MvnI CGCG 2 cut(s) 354, 957
MwoI GCNNNNNNNGC 1 cut(s) 901
NaeI GCCGGC 1 cut(s) 897
NcoI CCATGG 1 cut(s) 252
NdeII GATC 8 cut(s) 42, 87, 437, 484, 589, 689, 864, 1161
NgoMIV GCCGGC 1 cut(s) 895
NlaIII CATG 2 cut(s) 256, 664
NlaIV GGNNCC 5 cut(s) 235, 738, 930, 944, 1147
NmeAIII GCCGAG 1 cut(s) 1076
NruI TCGCGA 1 cut(s) 957
PaeR7I CTCGAG 1 cut(s) 937
PdiI GCCGGC 1 cut(s) 897
PdmI GAANNNNTTC 3 cut(s) 474, 668, 1078
PfeI GAWTC 4 cut(s) 427, 986, 1004, 1132
PflFI GACNNNGTC 2 cut(s) 242, 1033
PflMI CCANNNNNTGG 1 cut(s) 253
PinAI ACCGGT 1 cut(s) 1139
PleI GAGTC 1 cut(s) 411
PpsI GAGTC 1 cut(s) 411
PshBI ATTAAT 1 cut(s) 510
Psp124BI GAGCTC 2 cut(s) 938, 1097
Psp6I CCWGG 2 cut(s) 403, 575
PspGI CCWGG 2 cut(s) 403, 575
PspN4I GGNNCC 5 cut(s) 235, 738, 930, 944, 1147
PspPI GGNCC 2 cut(s) 172, 1137
PspXI VCTCGAGB 1 cut(s) 937
PstI CTGCAG 1 cut(s) 463
PstNI CAGNNNCTG 1 cut(s) 467
PsuI RGATCY 1 cut(s) 484
PsyI GACNNNGTC 2 cut(s) 242, 1033
PvuII CAGCTG 1 cut(s) 464
RruI TCGCGA 1 cut(s) 957
RsaI GTAC 3 cut(s) 119, 560, 1057
RsaNI GTAC 3 cut(s) 118, 559, 1056
RseI CAYNNNNRTG 1 cut(s) 597
Rsr2I CGGWCCG 1 cut(s) 1137
RsrII CGGWCCG 1 cut(s) 1137
SacI GAGCTC 2 cut(s) 938, 1097
SaqAI TTAA 5 cut(s) 510, 642, 744, 806, 837
Sau3AI GATC 8 cut(s) 42, 87, 437, 484, 589, 689, 864, 1161
Sau96I GGNCC 2 cut(s) 172, 1137
SchI GAGTC 1 cut(s) 411
ScrFI CCNGG 2 cut(s) 405, 577
SduI GDGCHC 4 cut(s) 236, 606, 938, 1097
SfaNI GCATC 1 cut(s) 536
SfcI CTRYAG 1 cut(s) 459
Sfr274I CTCGAG 1 cut(s) 937
SinI GGWCC 2 cut(s) 172, 1137
SlaI CTCGAG 1 cut(s) 937
SmiMI CAYNNNNRTG 1 cut(s) 597
SmlI CTYRAG 4 cut(s) 836, 923, 937, 1065
SmoI CTYRAG 4 cut(s) 836, 923, 937, 1065
Sse9I AATT 5 cut(s) 36, 643, 655, 707, 1084
SspMI CTAG 1 cut(s) 687
SstI GAGCTC 2 cut(s) 938, 1097
StyD4I CCNGG 2 cut(s) 403, 575
StyI CCWWGG 1 cut(s) 252
TaaI ACNGT 6 cut(s) 30, 225, 290, 632, 1060, 1078
TaqI TCGA 5 cut(s) 41, 800, 938, 1072, 1130
TasI AATT 5 cut(s) 36, 643, 655, 707, 1084
TatI WGTACW 1 cut(s) 117
TauI GCSGC 4 cut(s) 308, 311, 757, 832
TfiI GAWTC 4 cut(s) 427, 986, 1004, 1132
Tru1I TTAA 5 cut(s) 510, 642, 744, 806, 837
Tru9I TTAA 5 cut(s) 510, 642, 744, 806, 837
TscAI CASTG 3 cut(s) 33, 266, 397
TspDTI ATGAA 3 cut(s) 20, 661, 677
TspGWI ACGGA 3 cut(s) 773, 1038, 1118
TspRI CASTG 3 cut(s) 33, 266, 397
Tth111I GACNNNGTC 2 cut(s) 242, 1033
Van91I CCANNNNNTGG 1 cut(s) 253
Vha464I CTTAAG 1 cut(s) 836
VpaK11BI GGWCC 2 cut(s) 172, 1137
VspI ATTAAT 1 cut(s) 510
XapI RAATTY 1 cut(s) 707
XbaI TCTAGA 1 cut(s) 686
XhoI CTCGAG 1 cut(s) 937
XmiI GTMKAC 1 cut(s) 619
XmnI GAANNNNTTC 3 cut(s) 474, 668, 1078
XspI CTAG 1 cut(s) 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.